DR. CARLOS S.

LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

CMO no. 13 series of 2017

COURSE CODE COURSE TITLE

MLS 109 Clinical Chemistry 1 (Routine Clinical Chemistry)

Department: Medical Technology / MLS Revision Status:

Prepared by: Verified by:

DR. CARLOS S. DE LEON DR. GABRIEL J. CRUZ

Reviewed by: Approved by:

PROF. MAYLAINE M. DE LEON DR. CARLOS S. DE LEON

DCLC VISION 2025 DCLC aims to be one of the country’s leading colleges in providing quality education
DCLC MISSION To produce competent graduates who can contribute to nation building.
Service for the welfare and empowerment of others;
Excellence, comparable to highest standards, while having strong moral principles;
CORE VALUES Resilience in all challenges;
Virtue as a guiding principle, admired by others as God loving individuals;
Integrity, honesty and strong belief on one’s
Commitment in responding to the community’s needs; and
Empathy in valuing social fairness.
INSTITUTIONAL Graduates of Dr. Carlos S. Lanting College are globally comparative, competitive, and
OUTCOMES competent in their field of profession, with high level of humanistic values, critical thinking,
conceptual and technical skills in accordance with the standards of academic program and
industry requirements relevant to nation building.

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 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

PROGRAM The graduates have the ability to:

OUTCOMES a. Demonstrate technical competence in performance of clinical laboratory test in aid
of diagnosis, treatment, and management of diseases vis-à-vis biosafety and waste
management;
b. Demonstrate analytical and critical thinking skills in the workplace;
c. Engage in the collection, analysis, and projection of health information for
improving the health care management system;
d. Demonstrate inter-personal skills, leadership qualities, and ethical practice of the
profession;
e. Apply research skills in relevant areas of Medical Technology/Medical Laboratory
Science practice;
f. Participate in community-oriented activities;
g. Engage in life-long learning activities; and
h. Demonstrate effective teaching and communication skills.
COURSE The course deals with the quantitative measurement of biochemical substances found in
DESCRIPTION body fluids essentially blood. This involves the knowledge and understanding of the basic
concepts and principles of their metabolism, laboratory analyses, and pathophysiology.
Quality assurance and safety are given due emphasis.
CREDIT UNIT/S 5 units (Lecture: 3, Laboratory: 2)
CREDIT HOUR/S Lecture: 3 hours (54 hours per sem.)
Laboratory: 6 hours ( 108 hours per sem.)
PREREQUISITE Biochemistry, Human Anatomy and Physiology
COURSE CILO 1 Differentiate the various biochemical substances, their metabolism, actions,
INTENDED reference ranges and variables that may affect their analyses.
LEARNING CILO 2 Correlate test results with pathologic conditions.
OUTCOMES CILO 3 Perform basic laboratory calculations.
CILO 4 Practice quality assurance and laboratory safety.
CILO 5 Perform correctly laboratory assays used to measure concentration of specific
analytes.
CILO 6 Solve common problem encountered in clinical laboratory.
CILO 7 Manifest professionalism.

COURSE This course will be delivered entirely online through the course management system
STRUCTURE CANVAS and support apps like EDMODO and ZIP Grade. You will use your student account
to login to the course from the CANVAS. EDMODO AND ZIP GRADE Login Page. If you
have not activated your student account, please visit the Manage Your Account on our MLS
FB page to do so.
In CANVAS, you will access online lessons, course materials, and resources. At designated
times throughout the semester, we will participate in a blend of self-paced and group-paced
activities using and/or alternative Internet-based technologies. Activities will
consist of chat, blogs, discussion forums, email, journaling, blogging, wikis, and web posting.

COURSE POLICIES Netiquette is a set of rules for behaving properly online. Your instructor and fellow students
wish to foster a safe online learning environment. All opinions and experiences, no matter
how different or controversial they may be perceived, must be respected in the tolerant spirit

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 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

of academic discourse. You are encouraged to comment, question, or critique an idea but
you are not to attack an individual. Working as a community of learners, we can build a polite
and respectful course community.
COURSE As a student in this course you are expected to maintain high degrees of professionalism,
INTEGRITY commitment to active learning and participation in this class and also integrity in your
behavior in and out of the on line classroom.
COURSE All assignments for this course will be submitted electronically through CANVAS unless
ASSIGNMENTS otherwise instructed. Assignments must be submitted by the given deadline or special
permission must be requested from instructor before the due date. Extensions will not be
given beyond the next assignment except under extreme circumstances.
LECTURE
TIME FRAME COURSE OUTLINE
AUGUST 24,2020 – SEPTEMBER 19,2020
Day 1 Orientation
A. Introduction to the course contents
B. Course Requirements and Activities
C. Grading system and Policies on Absences, tardiness and examinations
Day 2 I.Introduction
1. Scope of Clin. Chem.
1.1 Overview
1.2 Importance
1.3 Role of Med.Tech.
1.4 Definition of Terms
Day 3 II. Laboratory Mathematics
1.Laboratory mathematics
1.1 Unit conversion
1.2 Per cent solution
1.3 Normality
1.4 Molarity
1.5 Dilutions
1.6 pH and pOH
Days 4-5 III. Specimen Collection and Processing.
1.Specimen
1.1 Types
1.1.1 Blood
1.1.2 CSF
1.1.3 Urine
1.1.4 Others
1.2 Collection and Labeling
1.3 Handling, transport processing, storage and preservation.

2.Specimen variables
2.1 Pre-analytical
2.1.1. Patient identification and preparation.

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 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

2.1.2 Anticoagulants and preservatives.

2.2. Analytical
2.3 Post analytical
Days 6 – 7 IV.Quality Management
1.Introduction
1.1. Importance
2. Definition of terms
3. statistics
3.1.Descriptive
3.1.1. Mean
3.1.2. Median
3.1.3. Mode
3.1.4. SD
3.1.5. CV
3.1.6. Variance
3.1.7. SEM
3.2.Inferential
3.2.1. T- tests
3.2.2. F – tests
4. Quality Control Charts
4.1. Levy-Jennings
4.2. Westgard Rules
4.3. Six-sigma
4.4. Others
5. Intra-Laboratory QC monitoring
6. Proficiency testing
7. Method collections
8. Issues and concerns
Day 8 FIRST PERIOD EXAMINATION (PRELIM)
SEPTEMBER 21,2020 – OCTOBER 16,2020
Days 1 – 2 V. Instrumentation
1.Review of instrumentation:
1.1Photometry
1.2Spectrophotometry
1.2.1 AAS
1.2.2 FES
1.2.3 Reflectance spectrophotometry
1.3Nephelometry
1.4Turbidimetry
1.5Flourometry
1.6Electrophoresis
1.7Chromatography
1.8Ultracentrifugation
1.9Chemiluminiscence
1.10Electrochemistry

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 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

1.11Immunochemistry
1.12Dry chemistry
2.Automation
3.Point of care testing
4.Current trends
Days 3 – 4 VI. Carbohydrates
1. Biochemistry
1.1. Classification
1.2. Metabolism
2. Specimen consideration
3. Glucose measurement
3.1 methods
3.2 reference range
4. clinical significance
4.1. diabetes mellitus
4.2. hyperglycemia
4.3. hypoglycaemia
4.4. other related diseases
5. tests of diabetis mellitus.
Days 5 – 6 VII. Proteins
A. Amino Acids
a. Structure
b. Classification
c. Functions
d. Metabolism
B. Proteins
e. Structure
f. Classification
g. Functions
h. Metabolism
C. Specimen Considerations
D. Methods of Analysis
i. Total Nitrogen
j. Total Protein
k. Fractionation and Quantitation of Specific Proteins
l. Electrophoresis
m. Immuno chemical Methods
n. Reference Range
E. Proteins in other Body Fluids
F. Clinical Significance
Days 7 VIII. Lipids and Lipoproteins
Lecture:
A. Lipids
a. Structure
b. Classification
c. Functions

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 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

d. Metabolism
B. Lipoproteins
a. Structure
b. Classification
c. Functions
d. Metabolism
C. Specimen Considerations
D. Methods of Analysis
a. Laboratory Methods
b. Reference Range
E.Clinical Significance
IX. Non-Protein Nitrogens
Lecture:
A. Biochemistry
a. Types
i. Urea
ii. Creatinine
iii. Uric Acid
iv. Ammonia
v. Others
b. Functions
c. Metabolism
B. Specimen Considerations
C. Methods of Analysis
a. Laboratory Methods
b. Reference Range
D. Clinical Significance
E.Kidney Function Test
Day 8 SECOND PERIOD EXAMINATION (MIDTERM)

LABORATORY
AUGUST 24,2020 – SEPTEMBER 19,2020
TIME FRAME COURSE OUTLINE
Day 1 Orientation and SOP’s
Day 2 1.Clinical Laboratory Apparatus and Supplies.
2. Volume Measurement.
Day 3 1.Preparation of Solutions and dilutions.
Days 4-5 1. Blood Collection
2. Skin Puncture
3. Venipuncture
4. Syringe method
5. Evacuated system
6. Arterial puncture
7. Preparation of serum, plasma, & whole blood
8. Laboratory safety

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 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

9. Laboratory waste mgt

Days 6 1.Preparation and interpretation of QC chart

2.Application of Westgard rules
1.Operation and maintenance of spectrophotometer and other laboratory instruments.
2.Preventive maintenance of laboratory instruments.
Day 7 1. Enzymatic methods for glucose.
2. HbA1C
3. Tolerance test
4. Laboratory safety
5. Laboratory waste management
Day 8 FIRST PERIOD EXAMINATION (PRELIM)
SEPTEMBER 21,2020 – OCTOBER 16,2020
Days 1 – 2 1. Methods of Protein Measurement
a. Total Protein
b. Albumin
c. Globulin & A/G ratio
Days 3 – 4 1. Total Cholesterol detn
2. Triglycerides detn
3. LDL/VLDL/
4. HDL detn
Day 5 – 6 1. Blood Urea detn.
2. Creatinine detn.
3. Blood Uric Acid detn.
Day 7 Virtual Practical Exam

Day 8 SECOND PERIOD EXAMINATION (MIDTERM

COURSE Attendance – 80% of the required class hours

REQUIREMENTS Class participation – discussions, examination etc.
APPROACH/ Lecture / discussion
METHODOLOGY Visual aids
Oral recitation
EVALUATION Recitation
Research work / Seat work
Examination
GRADING SYSTEM LECTURE 60 % LABORATORY 40 %
Recitation 10 % Attendance 10 %
Short Quiz 10 % Short Quiz 10 %
Long Quiz 20 % Class Standing – Experiment -10 %
Class Standing – Reporting 10 % Class Standing – Laboratory Manual -10 %
Class Standing – Research / Project -10 % Practical Exam 30 %
Major Examination- 40 % Theoretical Exam 30 %
Total = 100 % Total = 100 %

Note: Final Grade = First Period + Second Period / 2

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 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

REFERENCE 1. Anderson, Shauna and Susan Cockyane. Clinical Chemistry: Concepts and Applications.
US: Waveland Press Inc., 2007.
2. Arneson, W. and J. Brickell. Clinical Chemistry: A Laboratory Perspective. USA: F.A.
Davis Co., 2007.
3. Ashwood E., D. Bruns and C. Burtis. Tietz’s Fundamentals of Clinical Chemistry 6th ed.
Philadelphia: W.B. Saunders Co., 2007.
4. Ashwood E., D. Bruns and C. Burtis. Tietz’s Textbook of Clinical Chemistry and Molecular
Diagnostics 4th ed. Philadelphia: W.B. Saunders Co., 2007.
5.Bishop, Michael L. et.al. Clinical Chemistry: Principles, Procedures, Correlation’s, 5th ed.
Philadelphia: Lippincott Williams, Philadelphia, 2005.
6.Crook, Martin. Clinical Chemistry and Metabolic Medicine 7th ed. USA: Hodder Arnold
Publication, 2006.
7.Furr, Keith. CRC Handbook of Laboratory Safety 5th ed. USA: CRC, 2000.
8.Garza, Diana and Kathleen Becan-McBride. Phlebotomy Handbook: Blood Collection
Essentials 7th ed. USA: Prentice Hall, 2004.
9.Helms, Joel R. Mathematics for Medical and Clinical Laboratory Professionals. USA:
Delmar Learning, 2008.
10. Hibbert, Brynn. Quality Assurance in the Analytical Chemistry Laboratory. USA: Oxford
University Press, 2007.
11. Hoeltke, Lynn. Phlebotomy: Principles and Procedures. USA: Delmar Learning, 2006.
12. Kaplan, A., A. Pesce and S. Kazmierczak. Clinical Chemistry: Theory, Analysis,
Correlation 4th ed. Edinburgh: Mosby, 2002.
13. Lewandrowski, Kent. Clinical Chemistry: Laboratory Management and Clinical
Correlations. USA: Lippincott Williams and Wilkins, 2002.
14.Marshall, William and Stephen Bangert. Clinical Chemistry 5th ed. Edinburgh: Mosby,
2004.
15.McClatchey, Kenneth. Clinical Laboratory Medicine 2nd ed. USA: Lippincott Williams and
Wilkins, 2002.
16.Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and
Management by Laboratory Methods 21st ed. Philadelphia: Elsevier Inc., 2007.
17.Prichard, Elizabeth and Victoria Barwick. Quality Assurance in Analytical Chemistry.
USA: Wiley-Interscience, 2007.
18.Sacher, Ronald and Richard McPherson. Widmann’s Clinical Interpretation of Laboratory
Tests 11th ed. Thailand: F.A. Davis, 2000.
19.Scott M., A. Gronowski and C. Eby. Tietz’s Applied Laboratory Medicine 2nd ed. USA:
Wiley-Liss, 2007.
20.Wu, Allan. Tietz’s Clinical Guide to Laboratory Tests 4th ed. Philadelphia: W.B.
Saunders, 2006.

Electronic References:

1. http://webpages.chhs.niu.edu/williams/AHP318/ClinicalChem.htm
2. http://www.dgrhoads.com/links.shtml
3. http://www.kmcsystems.com/invitro_clinical.asp#1
4. http://www.ualberta.ca~intd410/departments/spoc.html
5. http://www2.apsu.edu/~thompsonj/clin-chem-page1.htm

Page 8 of 9
 DR. CARLOS S. LANTING COLLEGE
Medical Laboratory Science Department
16 Tandang Sora Ave., Sangandaan, Novaliches, Quezon City
Tel. No. (02) 938-7782 / (02) 938-7789 Telefax No. (02) 939-7229
Email: [email protected] Website: www.lantingcollege.edu.ph

CLASS ROOM CLASS BEHAVIOR that disrupts the learning process will not be tolerated. This includes:
POLICY talking to others during lecture, demonstrations, quizzes or exams. Sleeping or laying the
head down during class, using cell phones for any reason, noises from electronic devices,
wearing headphones, swearing and rude or disrespectful behaviour. Cell phone usage of
any kind will not be allowed in class. Please turn off or put on silent mode upon entering the
class. Cell phones shall not be used for computations. Courtesy, kindness and respect are
expected at all times from students and faculty.
ACADEMIC HONESTY: Cheating of any kind is a serious breach of personal morality and
an offense against fellow students, instructors/professors, program, and school. Any student
found to be academically dishonest will receive a Failing Grade and dismissed from the
program and school. Please refer to Student Handbook.
* The course schedule is subject to change with prior notice from the instructor.
* The major examinations schedule is subject to change with prior notice from the instructor.

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