This document provides instructions for preparing 1 M and 0.1 M sodium phosphate buffer solutions at various pH levels between 6.0-7.2 and 5.8-8.0 respectively. It details making stock solutions of monobasic and dibasic sodium phosphate and then mixing specified volumes of each stock to achieve the desired pH when diluted to 1 L with water. Tables list the volumes of each stock solution needed to prepare 1 L of buffer at different pH values within the given ranges.
This document provides instructions for preparing 1 M and 0.1 M sodium phosphate buffer solutions at various pH levels between 6.0-7.2 and 5.8-8.0 respectively. It details making stock solutions of monobasic and dibasic sodium phosphate and then mixing specified volumes of each stock to achieve the desired pH when diluted to 1 L with water. Tables list the volumes of each stock solution needed to prepare 1 L of buffer at different pH values within the given ranges.
This document provides instructions for preparing 1 M and 0.1 M sodium phosphate buffer solutions at various pH levels between 6.0-7.2 and 5.8-8.0 respectively. It details making stock solutions of monobasic and dibasic sodium phosphate and then mixing specified volumes of each stock to achieve the desired pH when diluted to 1 L with water. Tables list the volumes of each stock solution needed to prepare 1 L of buffer at different pH values within the given ranges.
This document provides instructions for preparing 1 M and 0.1 M sodium phosphate buffer solutions at various pH levels between 6.0-7.2 and 5.8-8.0 respectively. It details making stock solutions of monobasic and dibasic sodium phosphate and then mixing specified volumes of each stock to achieve the desired pH when diluted to 1 L with water. Tables list the volumes of each stock solution needed to prepare 1 L of buffer at different pH values within the given ranges.
Download as DOC, PDF, TXT or read online from Scribd
Download as doc, pdf, or txt
You are on page 1/ 4
Sodium phosphate
1 M sodium buffer (pH 6.0–7.2)
Mixing 1 designated in the table below results in 1 L phosphate of 1 M sodium phosphate buffer of the desired pH. To prepare the stock solutions, dissolve 138 g of NaH2PO4•H2O (monobasic; m.w. = 138) in sufficient H 2O to make a final volume of 1 L and dissolve 142 g of Na2HPO4 (dibasic; m.w. = 142) in sufficient H2O to make a final volume of 1 L.M NaH2PO4 (monobasic) and 1 M Na2HPO4 (dibasic) stock solutions in the volumes
Volume (mL) of 1 M Volume (mL) of
NaH2PO4 1 M Na2HPO4 Final pH
0.1 M 877 123 6.0 sodium
850 150 6.1
815 185 6.2
775 225 6.3
735 265 6.4
685 315 6.5
625 375 6.6
565 435 6.7
510 490 6.8
450 550 6.9
390 610 7.0
330 670 7.1
280 720 7.2
phosphate buffer (pH 7.4) Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C. 0.1 M sodium phosphate buffer (from 1 M stocks) at 25°C To prepare 1 L of 0.1 M sodium phosphate buffer of the desired pH, the following mixtures should be diluted to 1 L (final volume) with H2O. Volume (mL) of 1
