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    What Is Harada-Mori Culture?

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    VAN AERON PADULLO
    This document provides descriptions and importance of several diagnostic techniques for parasites and infectious diseases: 1) Harada-Mori culture and Baermann culture are used to culture nematode larvae like Strongyloides from stool samples. 2) Knott's concentration technique concentrates microfilariae in blood samples for identification like the heartworm Dirofilaria immitis. 3) Sheather's floatation and brine floatation techniques use solutions to separate parasite eggs and larvae from debris in stool samples. 4) McMaster and mini-FLOTAC techniques quantify parasite eggs per gram of stool to estimate worm burden and identify parasites like Ascaris lumbricoides.

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    What Is Harada-Mori Culture?

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    VAN AERON PADULLO
    272 views6 pages
    This document provides descriptions and importance of several diagnostic techniques for parasites and infectious diseases: 1) Harada-Mori culture and Baermann culture are used to culture nematode larvae like Strongyloides from stool samples. 2) Knott's concentration technique concentrates microfilariae in blood samples for identification like the heartworm Dirofilaria immitis. 3) Sheather's floatation and brine floatation techniques use solutions to separate parasite eggs and larvae from debris in stool samples. 4) McMaster and mini-FLOTAC techniques quantify parasite eggs per gram of stool to estimate worm burden and identify parasites like Ascaris lumbricoides.

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    Different Diagnosis Technique; Parasitology

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    30_PADULLO_OLA1

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    This document provides descriptions and importance of several diagnostic techniques for parasites and infectious diseases: 1) Harada-Mori culture and Baermann culture are used to culture nematode larvae like Strongyloides from stool samples. 2) Knott's concentration technique concentrates microfilariae in blood samples for identification like the heartworm Dirofilaria immitis. 3) Sheather's floatation and brine floatation techniques use solutions to separate parasite eggs and larvae from debris in stool samples. 4) McMaster and mini-FLOTAC techniques quantify parasite eggs per gram of stool to estimate worm burden and identify parasites like Ascaris lumbricoides.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    272 views6 pages

    What Is Harada-Mori Culture?

    Uploaded by

    VAN AERON PADULLO
    This document provides descriptions and importance of several diagnostic techniques for parasites and infectious diseases: 1) Harada-Mori culture and Baermann culture are used to culture nematode larvae like Strongyloides from stool samples. 2) Knott's concentration technique concentrates microfilariae in blood samples for identification like the heartworm Dirofilaria immitis. 3) Sheather's floatation and brine floatation techniques use solutions to separate parasite eggs and larvae from debris in stool samples. 4) McMaster and mini-FLOTAC techniques quantify parasite eggs per gram of stool to estimate worm burden and identify parasites like Ascaris lumbricoides.

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    of 6

    30_PADULLO, Van Aeron B.

    2G MT

    HARADA-MORI CULTURE

    What is Harada-Mori Culture?


    Harada-Mori Culture technique is a done by incubating stool sample/s on a filter
    paper strip in a test tube that is containing water for the purpose of culturing and
    recovering nematode larvae (Strongyloides). The water in the test tube should be covering
    one-third of the length of the paper strip.
    According to the research “The Laboratory Diagnosis and Follow Up of
    Strongyloidiasis: A Systematic Review”, Harada-Mori is a filter-paper technique that
    utilizes the water tropism of Strongyloides larvae to concentrate them
    Importance:
    To culture nematode larvae specifically Strongyloides larvae.

    BAERMANN CULTURE

    What is Baermann Culture


    Baermann culture is a culture technique based on the active migration or
    movements of larvae. This method was firstly described in 1917 as a cheap and simple
    technique based on the ability of S. stercoralis to enter a free-living cycle development.
    The stool sample is placed on a coarse fabric overlying a mesh screen in a funnel that is
    filled with warm water and connected to a clamped tube. After the incubation period,
    larvae are expected to crawl out of the fecal suspenson and migrate into the water from
    where they can be collected by centrifugation (Requena-Mendez, et al., 2013)
    Importance:
    Used to separate larvae from the stool sample. For the Diagnosis of lungworm
    infection and identification of third stage larvae (L3) from stool sample.
    KNOTT’S CONCENTRATION TECHNIQUE

    What is Knott’s Concentration Technique?


    A method used for the detection of microfilariae in the blood. This method is more
    sensitive than a direct smear with fresh blood as it concentrates the microfilariae.
    Importance:
    Identification of microfilariae, specifically the heartworm Dirofilaria immitis.

    SHEATHER’S FLOTATION TECHNIQUE

    What is Sheather’s Floatation Technique (SFT)?


    Uses a sugar solution to detect parasite’s eggs and larvae. It uses a solution that
    have a higher specific gravity than the organism to be floated so that the organisms rise
    to the top and the debris sink to the bottom.
    Importance:
    The importance of SFT is it separate debris to parasites and make it easier to
    detect parasites.
    BRINE FLOTATION TECHNIQUE

    What is Brine Floatation Technique?


    Brine Floatation Technique is an obsolete technique for concentrating parasitic ova
    from feces.
    Importance:
    Determination of parasitic ova from feces.

    McMASTER CONCENTRATION TECHNIQUE

    What is McMaster Concentration Technique?


    This technique uses a counting chamber which enables a known volume of fecal
    suspension to be examined microscopically. The quantities are chosen so that the fecal
    egg-count can be easily derived by multiplying the number of eggs under the marked
    areas by a simple conversion factor.
    Importance:
    Provides an estimate of worm burden by determining the number of nematode
    eggs per gram of feces. The advantage of this technique is that it is quick since the eggs
    are floated free of debris before counting.
    MINI-FLOTAC TECHNIQUE

    What is Mini-FLOTAC Technique?


    A new diagnostic method based on flotation of the eggs. Composed of two
    chambers that are placed in the base surmounted by a reading disc.
    Importance:
    A promising diagnosis for helminths, it is more sensitive than Kato-Katz ands
    McMaster for H. nana and as sensitive as Kato-Katz and more sensitive that McMaster
    for Ascaris lumbricoides identification.

    RAPID DIAGNOSTIC TEST FOR MALARIA

    What is Rapid Diagnostic Test for Malaria?


    Malaria rapid diagnostic tests (RDTs) assists in the diagnosis of malaria by
    providing evidence of the presence of malaria parasites in human blood. RDTs are an
    alternative to diagnosis-based on clinical grounds or microscopy, particularly where good
    quality microscopy services cannot be readily provided (WHO, 2015).
    Importance:
    Give quick tests for places where tests are in need specially if the place does not
    offer microscopy services.
    CIRCUMOVAL PRECIPITIN TEST (COPT)

    What is Circumoval Precipitin Test?


    A serological test used for diagnosis of Schistosomiasis japonica. Soluble egg
    antigens of Schistosoma japonicum block the formation of the circumoval precipitin by
    serum from infected humans
    Importance:
    Detection of Schistosoma japonicum eggs and for the diagnosis of Schistosomiasis
    japonica.

    SABIN-FELDMAN DYE TEST

    What is Sabin-Feldman Dye Test?


    A serological test to diagnose for toxoplasmosis. This test is based on the presence
    of certain antibodies that prevent methylene blue dye to enter the cytoplasm of
    Toxoplasma organism.
    Importance:
    Diagnosis of Toxoplasmosis.
    References:

    Requena-Méndez, A., Chiodini, P., Bisoffi, Z., Buonfrate, D., Gotuzzo, E., & Muñoz, J. (2013). The laboratory
    diagnosis and follow up of strongyloidiasis: a systematic review. PLoS neglected tropical diseases, 7(1), e2002.
    https://doi.org/10.1371/journal.pntd.0002002.

    Barda, B., Cajal, P., Villagran, E. et al. Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from
    north Argentina. Parasites Vectors 7, 271 (2014). https://doi.org/10.1186/1756-3305-7-271.

    Cringoli, G., Maurelli, M. P., Levecke, B., Bosco, A., Vercruysse, J., Utzinger, J., & Rinaldi, L. (2017). The Mini-FLOTAC
    technique for the diagnosis of helminth and protozoan infections in humans and animals. nature protocols,
    12(9), 1723.

    Modified knott’s technique and direct blood smear. (n.d.). Retrieved from https://sonopath.com/articles/modified-
    knott-s-technique-and-direct-blood-smear

    Sheather’s Sugar Flotation Solution. (n.d.). Retrieved from https://www.jorvet.com/wp-


    content/uploads/2012/01/sheathers_sugar_flotation_solution.pdf

    Mbong Ngwese, M., Prince Manouana, G., Nguema Moure, P. A., Ramharter, M., Esen, M., & Adégnika, A. A. (2020).
    Diagnostic Techniques of Soil-Transmitted Helminths: Impact on Control Measures. Tropical Medicine and
    Infectious Disease, 5(2), 93.

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