EN 14103 Determination of Total FAME and Linolenic Acid Methyl Ester in FAME With AC Biodiesel All in One Solution

APPLICATION NOTE
EN 14103 Determination of total FAME and

linolenic acid methyl ester in FAME with AC
Biodiesel All in One Solution
• Complies to EN 14103:2011 SOLUTION

• Included QC materials sample The AC Biodiesel All in One fully complies with EN
14103:2011. It combines all major Biodiesel
• Dedicated Software for Biodiesel
methods in one complete solution.
specific reporting The sample is introduced into a Split/Splitless inlet,
the capillary column separates the individual
Keywords: components that are subsequently detected by the
FID. Instrumental conditions are inserted in Fig 3.
FAME, EN 14103, All-in-one Biodiesel,
Calculation of the percentage of FAME is achieved
linolenic acid, FID-detection.
with internal standard calibration. The method is
suitable for FAME’s which contain methyl esters
between C6:0 and C24:1. A pre-diluted sample,
supplied with the solution contains all important
INTRODUCTION FAME components, as listed in Table 1 below.
Biodiesel is the “green” equivalent for petroleum Name Double

diesel or petrodiesel. Biodiesel is a renewable fuel bonds
derived from algae, vegetable oils, animal fats or Caproic or Hexanoic acid C6:0
cooking oils. The most important environmental Caprylic or Octanoic acid C8:0
benefit of biodiesel is that it is biological degradable, Capric or Decanoic acid C10:0
less poisonous and does not contain aromatics and Lauric or Dodecanoic acid C12:0
very low sulfur. Therefore, burning biofuels release Myristic or Tetradecanoic acid C14:0
less sulfur oxides and carbon monoxide. Myristoleic Tetradecenoic acid C14:1
Palmitic or Hexadecanoic acid C16:0
Quality requirements of biodiesel for use as pure Palmitoleic or Hexadecenoic acid C16:1
biofuel or blending stock for diesel fuel are defined Margaric or Heptadecanoic acid C17:0
in ASTM D6751 and EN 14214 specification. The Stearic or Octadecanoic acid C18:0
standards ensure that among others the following Oleic acid or Octadecenoic acid C18:1
important factors in the fuel production process are Linoleic or Octadecadienoic acid C18:2
satisfied: complete reaction, removal of glycerin, Linolenic Octadecatrienoic acid C18:3
absence of Poly Unsaturated Fatty Acids, removal Nonadecanoic acid C19:0
of alcohol and absence of free fatty acids. Method Arachidic or Eicosanoic acid C20:0
EN 14103 is prescribed for the determination of Eicosenic acid C20:1
total FAME’s (Fatty Acid Methyl Esters) and Arachidonic or Eicosatetraenoic acid C20:4
Linolenic Acid Methyl Ester (C18:3, figure 1) Eicosapentaenoic acid C20:5
content. Behenic or Docosanoic acid C22:0
Erucic or Docosenoic acid C22:1
. Clupanodonic or Docosapentaenoic acid C22:5
Docosahexaenoic acid C22:6
Lignoceric or Tetracosanoic acid C24:0
Nervonic or methyl c-15-tetracosenoic C24:1
acid
Figure 1: Linolenic acid (C18:3). Table 1: Peak Identification common fatty acids
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APPLICATION NOTE
Figure 2: Reference sample C6:0 – C24:1 Parameter Setting

Inlet S/SL
Injection Volume 1 µl
Split Ratio 1:100
Injection Temp 250 °C
Oven initial Temp. 60 °C
Oven initial Time 2 min
Ramp 1 10 °C / min
Figure 2: Reference sample C6:0 – C24:1 Final Temperature 1 200 °C
Ramp 2 5 °C / min
Final Temperature 2 240 °C
Final Time 7 min
Flow mode Constant flow
Flow 2 ml/min
Column dimensions 30 m x 0.25 mm x
0.25µm
Column type Polyethylene Glycol
Wax
Detector FID
Detector Temp 250 °C
H2 flow 35 ml/min
Air flow 350 ml/min
Make-up flow (N2) 20 ml/min
Figure 3: Rapeseed FAME sample
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APPLICATION NOTE
Parameter Method Measured

Repeatability Repeatability
(r) (r)
Ester content (%) 1,01 < 0.2
Linolenic acid (%) 0.0283 + < 0.1
0.0175X
Iodine value 0.5447 + < 0.2
(gr I / 100 gr 0.003X
sample
Table 2: Analysis specifications
Figure 4: Rapeseed Reference Sample report
IODINE VALUE CONCLUSION
According to Biodiesel Specification EN 14214, the The performance of the AC Biodiesel All in One
Iodine value can be calculated from the methyl ester is demonstrated for EN 1403:2011. All
distribution pattern obtained from EN 14103:2011 requirements as stated in the method are met.
results. For each FAME compound a factor to
calculate the iodine value is given according method The AC Biodiesel All in One is also an excellent
prEN 16300. Iodine value, displayed in g iodine / choice to analyze FAME feedstock according to
100 mg, is automatically calculated and reported. ASTM D6584, EN 14105, EN 14110, prEN 16300
and EN 15779. Its innovative dual programmable
oven design ensures optimal availability and
SPECIFICATION CHECK flexibility towards various methods as no column
changes are required when switching
Besides Iodine value, Total ester content and applications and the system is ‘always ready’,
Linolenic Acid Methyl ester content are reported regardless the method that’s needed for the
conform EN 14103:2011 and checked against sample .
specifications in EN 14214. All results obtained are
within the limits specified for this particular sample. The included reference materials & chemicals
and the dedicated reporting makes the AC
Biodiesel All in One very user friendly and easy
to use for fastest adoption in any biodiesel lab.
AC Analytical Controls® has been the recognized leader in chromatography analyzers for gas, naphtha and gasoline streams in crude
oil refining since 1981. AC also provides technology for residuals analysis for the hydrocarbon processing industry. Applications cover
the entire spectrum of petroleum, petrochemical and refinery, gas and natural gas analysis;
ACs Turn-Key Application solutions include the AC Reformulyzer ® , DHA, SimDis, NGA,
Hi-Speed RGA and Customized instruments.
00.00.185 2012/1 - © Copyright 2012 PAC L.P. All rights reserved www.paclp.com

EN 14103 Determination of Total FAME and Linolenic Acid Methyl Ester in FAME With AC Biodiesel All in One Solution

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APPLICATION NOTE

EN 14103 Determination of total FAME and

• Complies to EN 14103:2011 SOLUTION

Biodiesel is the “green” equivalent for petroleum Name Double

Figure 2: Reference sample C6:0 – C24:1 Parameter Setting

Figure 3: Rapeseed FAME sample

Parameter Method Measured

Table 2: Analysis specifications

Figure 4: Rapeseed Reference Sample report

IODINE VALUE CONCLUSION

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