Scribd Logo
Upload

Welcome to Scribd!

  • 20 views

    Lab 1: Microscopic: Microscopes Are Divided Into Two Categories

    Uploaded by

    Zainab Hasan
    The document describes the basic components and use of compound microscopes. It explains that Antony van Leeuwenhoek discovered microscopes in 1674 and used them to observe bacteria. Microscopes are divided into light and electron microscopes, with light microscopes further divided into bright-field and fluorescent types. The basic parts of a microscope like the objective lenses, stage, and condenser are identified. Steps for focusing with coarse and fine adjustments and using slides are provided. Making wet mounts and staining slides are also summarized.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Lab 1: Microscopic: Microscopes Are Divided Into Two Categories

    Uploaded by

    Zainab Hasan
    20 views4 pages
    The document describes the basic components and use of compound microscopes. It explains that Antony van Leeuwenhoek discovered microscopes in 1674 and used them to observe bacteria. Microscopes are divided into light and electron microscopes, with light microscopes further divided into bright-field and fluorescent types. The basic parts of a microscope like the objective lenses, stage, and condenser are identified. Steps for focusing with coarse and fine adjustments and using slides are provided. Making wet mounts and staining slides are also summarized.

    Original Title

    Lab 1

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document describes the basic components and use of compound microscopes. It explains that Antony van Leeuwenhoek discovered microscopes in 1674 and used them to observe bacteria. Microscopes are divided into light and electron microscopes, with light microscopes further divided into bright-field and fluorescent types. The basic parts of a microscope like the objective lenses, stage, and condenser are identified. Steps for focusing with coarse and fine adjustments and using slides are provided. Making wet mounts and staining slides are also summarized.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    20 views4 pages

    Lab 1: Microscopic: Microscopes Are Divided Into Two Categories

    Uploaded by

    Zainab Hasan
    The document describes the basic components and use of compound microscopes. It explains that Antony van Leeuwenhoek discovered microscopes in 1674 and used them to observe bacteria. Microscopes are divided into light and electron microscopes, with light microscopes further divided into bright-field and fluorescent types. The basic parts of a microscope like the objective lenses, stage, and condenser are identified. Steps for focusing with coarse and fine adjustments and using slides are provided. Making wet mounts and staining slides are also summarized.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    ‫قسم التحليالت المرضية‬ Practical Human Biology ‫إحياء بشري عملي‬

    Lab 1 : Microscopic
    Microscope discovered by Antony Van Leaven hock in 1674 (Holland )
    Which observed bacteria by his lens and named animalcules.

    (( He was an amateur grinder lens in Holland ))


    Microscope is an instrument used to study the cells and microorganisms .

    Microscopes are divided into two categories :


    1- Light Microscope
    2- Electron Microscope

    The light microscopes are subdivided into:


    1- Bright – field microscopes
    2- Fluorescent microscopes

    The Electron microscopes are subdivided into:


    1- scanning electron Microscope
    2- Transmission electron microscopes

    Head
    1-Ocular (eye pieces ) : Monocular 10x , Binocular 10x
    2- Revolving disc (nose pieces )
    3- Objective Lenses (4x, 10x, 40x and 100x)

    Arm
    1- Stage (Mechanical stage )
    2- Adjustment knob : Coarse focus adjustment , Fine adjustment
    3- Mechanical stage clip
    4-Condenser
    5-Diaphragm

    1rst Class 1 Prof.Dr. Ali H.Demin AL.Khafaji 2020


    ‫قسم التحليالت المرضية‬ Practical Human Biology ‫إحياء بشري عملي‬

    Base (foot)
    Illuminator Condenser

    How Use The Compound Microscope


    Use a compound Microscope :

    1-Always carry the microscope by holding the arm of the microscope


    with one hand and supporting the base with the other hand

    2- Place the microscope on a flat surface , the arm should be positioned


    toward you

    3- Look through the eye age piece , Adjust the diaphragm so that the light
    comes through the opening in the stage.

    4-Place a slide on the stage so that the specimen is in the field of view
    .Hold it firmly in place by using the stage clips .

    5- Always focus first with the coarse adjustment and the low- power
    objective lens .Once the object is in focus on low power , the high power
    objective can be used .Use only the fine adjustment to focus the high
    power .

    1rst Class 2 Prof.Dr. Ali H.Demin AL.Khafaji 2020


    ‫قسم التحليالت المرضية‬ Practical Human Biology ‫إحياء بشري عملي‬

    Make A wet Mount :


    Wet mount are named as such because the object to be viewed is prepared
    or mounted in water , follow these steps to make a wet mount :

    1- Obtain a clean microscope slide and a coverslip, Add drop or two of


    water to the center of the microscope slide.

    2- Place the specimen in the drop of water.

    3-Pick up the coverslip by its edges. Do not the surface of the coverslip.

    Stand the coverslip on its edge next the drop of water.

    4- Slowly lower the coverslip over the drop of water and the specimen .

    5- Make sure that object is totally covered with water if is not remove the
    coverslip, add a little more water and replace the coverslip.

    Stain a slide :
    Staining a slide can make it easier to view a specimen. Stains enhance
    contrast ant and can call out certain features . for example using iodine as
    a stain will cause carbohydrates in the specimen to become bluish –black
    in color .the following indicate one way to stain a microscope slide .

    1- Prepare a wet mount , as indicated in the steps on the previous page .

    2- Obtain the stain from your teacher .Using a dropper , place a drop of
    the stain at one end of the coverslip.

    3- Place a paper towel at the end of the coverslip opposite the stain .The
    towel will draw the stain under the coverslip staining the specimen .

    1rst Class 3 Prof.Dr. Ali H.Demin AL.Khafaji 2020


    ‫قسم التحليالت المرضية‬ Practical Human Biology ‫إحياء بشري عملي‬

    1rst Class 4 Prof.Dr. Ali H.Demin AL.Khafaji 2020

    You might also like