Lab # 3
AIM: To investigate the effects of osmosis on Irish potata tissues.
MATERIALS AND APPARATUS : beakers, petridish, knife, ruler, tile, blotting paper, salt
solution, potato strips, distilled water.
METHOD: See page ….. for drawing
1. Four uniformed strips of potato were bored, then each was
measured to a length of 3cm and cut. The measurement was
recorded in a table.
2. Each stip was placed into a labled petridish.
In petridish A , a cylindrical potato strip was placed in water.
In petridish B, a cylindrical potato strip was place in 5% salt
solution.
In petridish C, a cylindrical potato stip was place in 50% salt
solution.
In petridish D, a cylindrical potato stip was left uncovered,
exposed to air.
3. The cylindrical potato strips were left standing for two (2)
hours. They were then removed from the dishes and dried on
blotting paper.
4. Each strip was remeasured and the length recorded.
5. The texture of each strip was observed and description
recorded.
RESULTS: Title
Tissue Initial Length Difference Percentage Texture Texture of Weigh Weight
length after 2 in length change of potato after t after
(cm) hrs. (cm) potato before
before
(A) Water
(B) 5% salt
solution
(C) 50%
salt
soultion
(D) Air
DISCUSSION
(i) Define what is osmosis?
(ii) What is turgidity and faccidity of cells?
(iii) Explain why the potato strip in Petridish ‘A’ is the way it is after 2hours.
(iv) Explain why the potato strip in Petridish ‘B’ is the way it is after 2 hours.
(v) Explain why the potato strip in Petridish ‘C’ is the way it is after 2 hours.
(vi) Explain why the potato strip in Petridish ‘D’ is the way it is after 2 hours.
REFLECTION
CONCLUSION:
(I) A revisit of your Aim and your results should be noted in making a conclusion.
SUGGESTED
SKILL CRITERIA FOR ASSESSMENT MARK
MARK
Observation
Recording
(A) Acceptable language and expression
Reporting
(ORR) Correct tense and voice
1
Few and no grammatical errors
Osmosis
Potato
Lab.#3
(B) Observation and Recording
Adequate details of
1
Tables (Title)
Diagrams, all appropriately neat. 1
Observation of significant relevant changes 2
recorded, of the length, weight and texture.
Extent or degree of change recorded
1
Original and final condition compared of the length,
2
weight and texture.
Condition of control included
1
(C) Reflections
Influence of laboratory finding to real life 1
Applications in specific areas
10
SUGGESTED
SKILL CRITERIA FOR ASSESSMENT MARK
MARK
Manipulation (A) Use of basic laboratory equipment.
and
1. Careful use of Cork borer, ensuring that strips are
Measurement
M+M long enough and uniform. 1
Osmosis
Potato Lab.#3 2. Carefully measuring and cutting each cylinder
2
strip at equal lengths of 3cm
3. Labelling of petri dishes 1
4. Carefully pouring out salt solutions avoiding 2
spillage.
5. Carefully drying and handling of potato strips 1
6. Careful use of the scale, zeroing the scale with
2
petri dish; then adding potato and taking reading.
7. Proper timing of submerged cylinder strips. All strips
1
are placed in the labelled petri dishes at the same
time.
10