150 Series

User’s Manual

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 150 Series Table of Contents

Before Use............................................................................................. 3
Introduction.............................................................................................................3
Safety Precautions...................................................................................................4
Parts....................................................................................................... 5
M150........................................................................................................................5
Definition of Parts...................................................................................................6
Getting Started..................................................................................... 7
Assembly..................................................................................................................7
Operation.............................................................................................. 8
Setting Up................................................................................................................8
Focusing...................................................................................................................8
Adjusting The Diaphragm.......................................................................................8
Attaching a Camera / Changing Eyepieces.............................................................8
Setting the Stage’s Stop-Limit..................................................................................9
Maintenance/Precautions.....................................................................................10
Specifications...................................................................................... 11
149 Series Specifications........................................................................................11
Optional Accessories..............................................................................................12
Objectives...............................................................................................................13
Eyepieces................................................................................................................13
Technical Parameters........................................................................ 14
Electrical System....................................................................................................14
Technical Terms & Concepts.................................................................................15
Common Issues......................................................................................................16
Common Issues (Continued)................................................................................17

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 150 Series Before Use

Introduction

Congratulations on the purchase of your new AmScope microscope!

This manual is designed for the M150 series microscopse.

Please be sure to take a few minutes to familiarize yourself with the features and functions of your
new AmScope microscope.

If you’d like more information on microscopes, parts, or accessories, please visit our website at:

www.iScopeCorp.com

We highly recommend you study this manual thoroughly before operating the microscope, and
that yo ukeep it on ahnd for future reference.

If you have additional questions or need assistance, please send us an email at:

[email protected]

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 150 Series Before Use

Safety Precautions

1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transporation. Do not shake the package.

2. Do not place the microscope in direct sunlight or in high heat. Keap it indoors in a dry
and clean place with temperateures between 32-100 degrees F (0-40 degrees C), and in
maximum relative humidity of 85%.

3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your fingerprints do not obstruct your view.

4. Before turning the power on, make sure that the power supply voltage is consident with
the voltage of your microscope.

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 150 Series Parts
M150

Eyepiece

Ocular Tube

Rotating Monocular Head

Nosepiece Turret

Objectives

Limit Stop

Stage Clips
Stage

Disc Diaphragm (Disc Iris) Fine Focusing

Knob

Coarse Focusing
Illuminator Lens Knob

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 150 Series Parts
Definition of Parts
Base Illuminator Lens
Directs the light source towards the slide

Focusing Knob
Used to bring the slide into the sight and focus

Disc Iris Diaphragm

Controls the amount of light that hits the slide from the base illuminator

Dimmer
Controls the amount of light that escapes from the base lens

Limit Stop Knob

Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective

Nosepiece
Houses the objective lenses

Rotating Monocular Head

Houses the eyepiece and optics of the microscope

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 150 Series Getting Started

Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which
side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the
optical items. Check carefully to ensure that all parts and accessories are intact.

2. Check the packing list to ensure that you’re received all items:
- One Monocular Microscope Body & Head
- Three Objectives (4x, 10x, 40x)
- 10x Widefield Eyepieces
- 16x Widefield Eyepieces (for -A models only)
- 20x Widefield Eyepieces (for -B models only)
- 25x Widefield Eyepieces (for -C models only)
- One Dust Cover

Note: LED models do not have a spare bulb, as the LED bulbs do not need replacing.

3. Remove the microscope body from the box and remove the plastic protective covering. The body of the micro-
scope is composed of the base, the stage, the arm, and the nosepiece.

4. Screw the objectives into the microscope nosepiece from the lowest magnification to the highest, again avoid-
ing touching the lenses.

5. Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob on
the side of the base.

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 150 Series Operation

Setting Up
1. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly
in place with the metal slide holders (clips) of the mechanical stage.

2. Center the specimen over the stage opening, lining it up with the light and the objective lens.

3. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of
light is achieved.

Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to lo-
cate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each
time.

2. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use
the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.

Note: You may loosen the limit-stop knob (located on the read of the stage) in order to give yourself the full range
of motion for fine tuning the focus.

3. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results.

Note: Please be careful when moving the mechanical stage if you need to recenter the sample, or if moving the
stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when
it is off you will be able to damage the microscope.

Adjusting The Diaphragm

1. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the
aperture of the iris diaphragm by rotating the disc underneath the stage to the desired aperture.

Attaching a Camera / Changing Eyepieces

1. To remove the 10x eyepiece included on the microscope, you will need to remove the small metal screw on the
ocular tube just under where the eyepiece connects to the tube. To do so, you will need a 1mm flathead precision
screwdriver (much like what you would use to adjust eye glasses).

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 150 Series Operation

Setting the Stage’s Stop-Limit

1. To adjust the stop limit on the stage, unlock it by unscrewing the lower nut. The stop limit is located at the rear
of the stage.

2. Once tension is released on the nut by rotating it counter clockwise, you may adjust the upper thumb screw to
move the limit up or down as desired.

3. Lock the limit stop nut back into place after the desired stage height is achieved.

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 150 Series Operation

Maintenance/Precautions
- All glass surfaces must be kept clean. Fine dust on the optical surface should be blown off using a can of com-
pressed air or gently wiped off with a soft lens paper tissue/nonabraisive lint free cloth.

- Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens
cleaner (we recommend Sparkle brand optical cleaner).

- Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted
surfaces.

- Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of
our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so.

- After use, cover the microscope with the provided dust cover.

- Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damage.

- To change batteries with this unit, use the included allen wrench (hexagonal shaped L-tool) to undo the door hex
screw on the base. Use 3 AAA batteries with the unit.

- The unit can be used as a recharger for AAA batteries specified as rechargeable only. Please do not use standard
batteries in the unit with the charger plugged in to avoid damage.

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 150 Series Specifications

150 Series Specifications

Parts Specifications M149 M149A M149B M149C
WF Eyepiece WF10X/18mm
WF10X/18mm w/ Pointer x x x x
WF10X/18mm w/ Reticle
WF16X/18mm x
WF20X/18mm x
WF25X18mm x
Plan Eyepiece P5X
P10X
P16X
DIN Achromatic Objectives 4X/0.10 x x x x
10X/0.25 x x x x
40X(spring)/0.65 x x x x
60X(spring)/0.85
100X(spring, oil)/1.25
Plan Objectives 4X
10X
40X(spring)
100X(spring, oil)
45 Degree Viewing Head Binocular Sliding, 360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling
Monocular, 360 Degree Swiveling x x x x
Diaphragm Iris Diaphragm Disc x x x x

Illumination Halogen Light w/ Dimmer

LED Light w/ Dimmer x x x x
Lamp 6V/20W
6V/30W
LED x x x x
Filter Blue/Yellow/Green

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 150 Series Specifications

Optional Accessories
Parts Description Model # Purpose
Eyepiece 5X EP5X23 Obtaining 20x, 50x, 200x, and 500x magnification
powers
20x EP20X23 Obtaining 80x, 200x, 800x, and 2000x magnifica-
tion powers
25x EP25X23 For obtaining 250x and 2500x magnification pow-
ers
10x w/ Pointer EP10X23P For easier identifying of objects
10x w/ Reticle EP10X23R For measuring objects
Objective 2X A2X For obtaining 20x and 32x magnification powers
5X A5X For obtaining 50X and 80X magnication powers
20X A20x For obtaining 200x and 320x magnification powers
60X A60X For obtaining 600x and 960x magnification powers
Plan 4X PA4X For obtaining higher clarify in images
Plan 10X PA10X For obtaining higher clarify in images
Plan 40X PA40X For obtaining higher clarify in images
Plan 100X PA100X For obtaining higher clarify in images
Camera CMOS Digital MU035 (350k) To capture images, video, or view live display on a
MU130 (1.3mp) computer (PC/Mac OS X)
MU300 (3mp)
MU500 (5mp)
MU800 (8mp)
MU900 (9mp)
MU1000 (10mp)
Calibration Micrometer MR400 To calibrate the camera software for on screen
measurements
CCD TV/Video (Trinocular CCD-NP To view live display on a television (RCA)
Only)
Case Aluminum Case AC-B100 For carrying microscope around safely

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 150 Series Specifications

Objectives
Type Magnification Numerical Aperture Medium Parfocal Distance (mm) Magnification Marks
(N.A.) (Color Ring)
DIN Achromatic 4X A2X Air 45 Red
Objective (195mm) 10X A5X Air 45 Yellow
40X A20x Air 45 Light Blue
60X A60X Air 45 Deep Blue
100X A100X Cedar Oil 45 White
Plan Objective Plan 4X PA4X Air 45 Red
(195mm) Plan 10X PA10X Air 45 Yellow
Plan 40X PA40X Air 45 Light Blue
Plan 100X PA100X Cedar Oil 45 White

Eyepieces
Type Widefield Eyepiece Plan Eyepiece
Medium
Magnification 10X 15X 20X 5X 10X 16X
Field of View Φ18 Φ13 Φ11 Φ18 Φ18 Φ15

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 150 Series Technical Parameters

Electrical System
There are two options for eletrical systems for this series of microscope. The light source is an LED system.

1. 220V~240V power supply: 220V~240V ±10%, 50Hz

This electrical system is CE and GS certified

2. 100V~120V power supply: 100V~120V ±10%, 60Hz

This electrical system is UL certified.

All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent
on which unit is purchased.

This unit is also capable of using batteries and charging rechargable batteries. It uses 3 AAA batteries. When
plugged in with batteries in the unit, the batteries will be charged.

Note: Please do not use the recharging feature with standard batteries, as damage to your unit can occur.

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 150 Series Technical Parameters

Technical Terms & Concepts

Total Magnification
Total magnification of a microscope is calculated by the magnification of the objective multiplied by the
magnification of the eyepieces.
-Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification

Field of View
Linear field of view of the eyepiece divided by the magnification of the objective

Numerical Aperture (N.A)

Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the fe-
taures of the objective and condenser’s image quality and resolution. The “n” is a refractive index of the medium
(air or immersion cedar oil) between the omjective lens and the specimen. The “α” is 1/2 of the angle between the
aperture on the objective and path of light. The larger the N.A, the higher the resolution of the objective (and better
quality of the image).

Object to Primary Image Distance

The distance between the object plane and the primary image plane. The conjugate distance is fixed.

Mechanical Tube Length

The distane between the objective shoulder and the ocular shoulder

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 150 Series Troubleshooting

Common Issues
Symptom Cause Remedy
OPTICAL ISSUES
One side of the field The nosepiece if misaligned Turn the nosepiece until it clicks into place
of view is darker Stains or dust has accumulated on the objective, Clean all lenses with lens cleaner or a lint free non-
eyepieces, or base lens abraisive cloth
Obstructions are Stains, dust, or dirt has accumulated on the speci- Clean the slide or use a new specimen if sample is
observed in the field men destroyed
of view Stains, dust, or dirt have accumulated on the lens Clean the lens
Unclear Image There is no cover slip on the slide Add a cover slip. The objectives are designed for use
with a 0.17mm cover slip, so it is a requirement to
use one for proper images.
The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm
thickness slip
The aperture is not open to an appropriate diam- Adjust the aperture to have the light just larger than
eter the size of the condenser
Stain or dust has accumulated on the lens in the Clean the lens with lens cleaner or a nonabraisive
inlet of the head lint free cloth, as well as spray with compressed air
One side of the field The specimen slide is not fixed Secure the slide to the stage with clips
of view is dark or the The nosepiece is not in the right position Turn the nosepiece until it clicks into place
image moves while
focusing
The field of view is not The iris disc diaphragm is not big enough Rotate the iris disc diaphragm to allow more light
bright enough to travel
Stains, dust, or dirt has accumulated on the con- Thoroughly clean tall lenses with lens cleaner or a
denser, objective, eyepieces, or base lens lint free nonabraisive cloth

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 150 Series Troubleshooting

Common Issues (Continued)

Symptom Cause Remedy
OPTICAL ISSUES
The color of the image is The brightness adjustment knob is not in the right position Adjust the brightness knob to a higher or lower setting for color
not accurate clarity
No filter is used or filter is in use Remove color filter if natural light is desired, or insert desired
filter
MECHANICAL ISSUES
The objective touches the The cover slip is not standard sized Replace the cover slip with the appropriate 0.17mm thickness slip
cover slip
The limit-stop is set too high or not engaged Be careful to avoid contact between objective and the slide when
the limit stop is not engaged. To reengage, focus the sample, then
lock the limit stop into place to set max height at a safe but us-
able distance.
Unable to move the slide The slide is not secured correctly Adjust the slide to use the stage clips and secure the sample
smoothly
The mechanical stage is not properly secured Tighten the mechanical stage screws to better secure the stage
Focus knob does not turn The tension knob is too tight Loosen it by adjusting the tension ring inside the coarse focus
knob counterclockwise (close to the arm of the microscope on
the left of the microscope)
Stage declines by itself The tension knob is too lose Tighten it by adjusting the tension ring inside the coarse focus
knob clockwise (close to the arm of the microscope on the left
of the microscope)
The focusing knob won’t Limit-stop is engaged Disengage the limit stop on the rear of the stage of the micro-
raise the stage scope
ELECTRICAL ISSUES
The bulb/light source The bulb is close to burning out Please contact us in regards to this issue. LED lights do not burn
flickers out, so there may be another electrical issue
The microscope does not The microscope is unplugged Insert the plug into the wall socket to achieve electrical illumina-
light up tion

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