RBC/PLT

1. Verify AUTO box is checked.

VP-03 Electronics Gain Adjustment Version - 202667-102_3852_5 Gain
2. Verify bead size is correct, if not-enter correct sizes from bottle
Adjustment label in the DIAMETER field [1].
3. Prepare the RBC/PLT bead mixture by combining 6 drops of
Inspect tools for damage, ensure calibration is not expired and replace if necessary. 5೦m beads with 4 drops of 3೦m beads in a tube.
4. Shake the RBC/PLT mixture with a vortex system.
5. Place the tube containing the well-mixed RBC/PLT bead mixture
At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument under the sample probe.
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary. 6. Click [RBC PLT GAIN].
7. At the end of the cycle, verify the channel recovery value is
within +/- 2 channels from the target channel, and the following:
VP-03 Electronics Gain Adjustment
RBC GAIN: 210 - 250 [2]
PLT GAIN: 210 - 250 [2]

Purpose Module CELL-DYN

To adjust the WBC and RBC/PLT gain. Emerald
Perform this procedure: Verification
1. Click [CONFIRM].
After replacing Counting Chamber or Counting Head 2. Click YES when the WARNING window appears, to save the
CPU adjustments.
Preamp
Note: To exit the screen without changing the value(s), click
[ESC].
Materials Time 30 min.
Required Standard Reference Particles (SRP):
8160601101 MICROSPHR POLYMER 5.0 DIA EMLD 3. Log in as Operator and run background.
4. Verify calibration by running controls and calibrate, if necessary.
8160601201 MICROSPHR POLYMER 3.0 DIA EMLD

8160601301 MICROSPHR POLYMER 7.0 DIA EMLD

Note: An RBC/PLT SRP mixture of 6 drops of 5µm +4 drops of 3µm spheres is used in
the Gain Adjustment process.
CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of
Note: The Standard Reference Particles (SRP) part numbers listed above are specific for use on the CELL-DYN Emerald. Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.

Note: Instructions are for software version 2.1.0 and above.

Action Steps Reference

Preparation
1. Logon. Use the field service password: AFSE.
2. From the MAIN MENU, click
[MAINTENANCE]
[ABBOTT SERVICE]
[SET GAINS].

WBC Gain
1. Verify AUTO box is checked.
Adjustment
2. Verify bead size is correct. If not, enter correct value from bottle
label in the DIAMETER field [1].
3. Shake the 7.0 microspheres with a vortex system.
4. Place the well-mixed 7 µm undiluted bead solution under the
sample probe.
5. Click [ WBC GAIN] .
6. At the end of the cycle, verify the channel recovery value is
within +/- 2 channels from the target channel [2], and the WBC
GAIN is within 210 - 250 [3].
7. Continue with Action: RBC/PLT Gain Adjustment or
proceed to Action: Verification.

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 Download to
2. Connect the male end Serial Cable (Null Modem) to
VP-04 Software Upgrade Version - 202667-102_4198_3 Instrument the Serial Port labeled 1010 RS232 of the Emerald.
3. Connect the female end of the cable to a Serial
Com of the PC.
Inspect tools for damage, ensure calibration is not expired and replace if necessary.
Launch Software
1. Power on the Emerald.
Upgrade
At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument 2. From the PC, open the EmeraldLoader-VXXX-
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary. XXX folder from the location noted in Action:
Download Software Upgrade to your Computer,
Step 1.
3. Open (Double Click) the EmeraldLoader-VXXX
VP-04 Software Upgrade
file. (Blue Icon)

The tool scans the PC Serial Com Port to

automatically find the Com Port connected to the
Purpose Module CELL-DYN Emerald.
Software upgrade instruction for the CD-Emerald analyzer. This document Emerald When the Com Port is found, the [Update] icon is
contains two (2) software download methods. Download method used depend displayed.
on the instrument೦s software revision level.
4. Click [Update]. The bar graph shows completion
Download Method 1: Personal Computer (PC), Serial (Null Modem) percentage.
Cable is used if the software version upgrade is not compatible with the 5. After successful completion of the software
upgrade, the instrument reboots.
current instrument software version level.
6. Power off the instrument after a successful reboot.
Download Method 2: Personal Computer (PC), USB Thumb Drive is 7. Close the EmeraldLoader application on the PC
used if the software version upgrade is compatible with the current and remove the Serial (Null Modem) Cable from the
instrument software version level. CD-Emerald instrument and the (PC).

Materials Required Time 60 min.

QTY-01 Download Method 2: Personal Computer (PC), USB Thumb Drive
Personal Computer (PC)
08H54-01 QTY-01 (Or Equivalent)
Serial Wire (Null Modem) Cable Action Steps Reference

or:
Format Thumb
1. Power on the instrument.
QTY-01 Drive
2. Logon to the system and use the field service
Personal Computer (PC) password: AFSE.
QTY-01 3. Click [SET UP].
USB Thumb-Drive (Do not use USB device larger than 2 GB.) 4. Click [ADVANCED].
5. Click [OTHER SETTINGS].
6. Click [STORAGE].
Download Method 1: Personal Computer (PC), Serial (Null Modem) Cable 7. Click [FORMAT EXT. STORAGE DEVICE].
8. Insert the Thumb Drive into the USB Port on the
back of the instrument.
Action Steps Reference 9. Click [YES].

Note: The AB18 Folder (with sub-folders) copies to

Software the Thumb Drive.
1. Create a folder on Drive ‘C’ label EmeraldLoader-
Upgrade
VXXX-XXX.
Download 10. Click [ESC] and remove the Thumb Drive.
(VXXX-XXX = Software Upgrade version)
2. Locate the Attachments section of the Software
Upgrade ISA/TSB.
3. Copy each file below the Software VXXX-XXX
DNLD INST file to the folder created in Step 1.

Note: The EmeraldLoader-VXXX-XXX folder and

associated files can be used in both methods of software
upgrade.

Software
1. Power off the instrument.

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 EmeraldLoader-VXXX-XXX.
Upgrade (VXXX-XXX = Software Upgrade version)
2. Locate the Attachments section of the Software
Upgrade TSB.
3. Open all four (4) files directly below the Software
VXXX-XXX DNLD INST.doc and save them to the
folder created in Step 1.

Note: The EmeraldLoader-VXXX-XXX folder and

associated files can be used in both methods of software
upgrade.

Copy Software
1. Insert the Thumb Drive into either USB Port of the
Upgrade to
PC.
Thumb Drive 2. Locate and open the EmeraldLoader-VXXX-XXX
folder on the PC.
(VXXX-XXX = Software Upgrade version)
3. Highlight and copy the AB18Release-X.X.X-
XXXX.b file.
(X.X.X-XXXX= Software Upgrade version)
4. Open the Thumb Drive (usually Drive E:).
5. Double-click [AB18] folder.
6. Double-click [UPDATE] folder.
7. Double-click [VERSION] folder.
8. Paste the AB18Release-X.X.X-XXXX.b file into the
[VERSION] folder.
9. Remove the thumb Drive from the PC.

Launch 1. With the power off, connect the Thumb Drive to the
Software USB Port on the back of the CD-Emerald
Upgrade instrument.
2. Power on the instrument.
3. Log on to the system. Use the field service
password: AFSE.
4. Click [SET UP].
5. Click [ADVANCED].
6. Click [VERSION RELEASE].
7. Click AB18RE ~ 1.
8. Click [LOAD]. After a message with information
confirming a successful upgrade has been
displayed, remove the thumb drive.
9. Click [OK]. The system will reboot.
10. LOG ON to the system and use the field service
password AFSE.
11. Click the date and time icon in the upper left corner
to verify the software version.

Download
1. Create a folder on Drive ‘C’ of the computer called
Software

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 VP-05 Touch Screen Calibration Version - 202667-102_4199_3

Inspect tools for damage, ensure calibration is not expired and replace if necessary.

At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.

VP-05 Touch Screen Calibration

Purpose Calibrate LCD display touch screen on CELL-DYN Emerald. Module CELL-DYN Emerald
Materials Required None Time 3 min.

Action Steps Reference

Calibrate
Touch Screen 1. At LOGIN SCREEN press the ENTER key two (2) times.
2. Invoke Touch Screen Calibration by simultaneously pressing the “.”
key and the " DEL” key on the numerical keyboard.
3. Touch the center of the cross shown on the screen.
4. Repeat Step 2 for two more screen locations. When complete, the
Touch Screen Calibration returns to the original menu.

Verification Navigate through the menus to verify the touch screen response.

CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of
Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.

CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of
Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.

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 VP-06 Pressure/Vacuum Leak Detection Version - 202667-102_4214_2 CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of
Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.

Inspect tools for damage, ensure calibration is not expired and replace if necessary.

At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.

VP-06 Pressure/Vacuum Leak Detection

Purpose To detect pressure or vacuum leak in the Syringe Module Assembly on CELL-DYN Module CELL-DYN
Emerald. Emerald
Materials Flat-head screwdriver Time 3 min.
Required

Action Steps Reference

Prerequisite
1. Verify the Analyzer is in Ready State. (The LED above
the aspiration area is green.)

2. Log in with the service (AFSE) password.

3. Using the flat-head screwdriver, turn the two screws

securing the right door counter-clockwise and open the
right side door.

Verification
of Pressure
1. Initiate a cycle run by pressing the start switch plate.
Stability
2. Observe the syringe pistons and interrupt the cycle
(when the pistons are positioned half-in or half-out of
the syringe body) by pressing the ON/OFF button on
the front panel.
3. From the Main Menu, select
MAINTENANCE>MECHANICAL CHECKS>CHECK
SENSORS..
4. Verify the valve in the PRESSURE field is stable and
does not decrease over time. [1]

Note
The value may fluctuate, but should not
decrease over time.

5. If the PRESSURE value decreases, troubleshoot the

leak by checking the tubing and syringe block.
6. If the PRESSURE value is stable, close the right side
door. Return to the MAINTENANCE menu and press
INITIALIZE SYSTEM.
7. Return to the Main Menu and press START UP. Log
out of the service login.

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 VP-07 Set Serial Number Version - 202667-102_4213_2

Inspect tools for damage, ensure calibration is not expired and replace if necessary.

At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.

VP-07 Set Serial Number

Purpose To enter and save the analyzer Serial Number information, e.g., after Main Board Module CELL-DYN
replacement. Emerald
Materials None Time 3 min.
Required

CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of
Action Steps Reference Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.

Preparation Log in with the service (AFSE) password.

Verfication of
Pressure
1. From the Main Menu, click
Stability
MAINTENANCE>ABBOTT SERVICE>SET
SERIAL NUMBER.

2. Locate the label with the Serial Number on the

rear panel of the analyzer. Type the Serial
Number in the fields. [1]

3. Click CONFIRM and YES to save the

information.

4. Return to the Main Menu and log out of the

service login.

Verification
1. Click the Date/Time icon in the top-left corner
of the window.
2. Verify that the ID information displays the
correct analyzer serial number.

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VP-08 CELL-DYN Emerald Pressure Sensor Calibration VersionBB

Inspect tools for damage, ensure calibration is not expired and replace if necessary.

At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.

VP-08 CELL-DYN Emerald Pressure Sensor Calibration

Purpose 7R&DOLEUDWHWKH&383UHVVXUH6HQVRURQWKH&(//'<1 Module &(//'<1(PHUDOG

(PHUDOG
Materials Time ,QVWDOODWLRQ9HULILFDWLRQRIWRRODQGFDEOH
Required PLQ
/DSWRS&RPSXWHU
&DOLEUDWLRQRIWKH3UHVVXUH6HQVRUPLQ
86%WR6HULDO&DEOHSDUW
&'(PHUDOG3UHVVXUH6HQVRU&DOLEUDWLRQ7RRO

Action Steps Reference &DOLEUDWHWKH3UHVVXUH &DOLEUDWLRQ3UHVVXUH6HQVRU7RROLFRQ

6HQVRULQWKH(PHUDOG
 'RXEOHFOLFNRQWKH
3RZHU2QWKH&' LQVWUXPHQW
&$/,%5$7253UHVVXUH
(PHUDOGLIQRWWXUQHGRQ 6HQVRU&DOLEUDWLRQ7RROLFRQ
 8VHWKH2Q2IIVZLWFKRQWKH Note
DOUHDG\ RQ\RXUODSWRSFRPSXWHU
IURQWRIWKHLQVWUXPHQW In order for the
Pressure Sensor  ³&KHFNLQJDXWRPDWRQ
Calibrator screen FRQQHFWLRQ´PHVVDJHZLOO 3UHVVXUH6HQVRU&DOLEUDWLRQVFUHHQ
to show the DSSHDU
bottom message,  ,IFRPPXQLFDWLRQEHWZHHQWKH
Windows 7 and &'(PHUDOGLQVWUXPHQWDQG
other Windows \RXUODSWRSFRPSXWHULV
users may have VXFFHVVIXOWKHPHVVDJH
to change their Automaton was found on
Appearance and port COM3 1 ZLOODSSHDUDWWKH
Personalization ERWWRPRIWKHVFUHHQ
settings to
Windows Classic.  ,QWKLVH[DPSOHSRUW&20LV
FRQILJXUHG<RXUSRUWORFDWLRQ
PD\GLIIHUEDVHGRQ\RXU
FRPSXWHUSRUWDYDLODELOLW\
 ,IWKHPHVVDJHGRHVQ
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(VWDEOLVK /,6&RQQHFWLRQ56& ODSWRSFRPSXWHUDQG
FRPPXQLFDWLRQEHWZHHQ LQVWUXPHQWDQGRUHQVXUH
 8VLQJWKH86%WR6HULDOFDEOH LQVWUXPHQWLVWXUQHGRQ
\RXUODSWRSDQGWKH&'
SDUWFRQQHFW  2QFHWKHFRPPXQLFDWLRQKDV
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WKH/,6&RQQHFWLRQ56& PHVVDJH“Automaton
LQWKHEDFNRIWKHLQVWUXPHQW XXXXXX-XXXXXX will be
DQGWKH86%HQGRIWKHFDEOH calibrated. Enter the current
WRWKH86%SRUW\RXKDYH altitude of the instrument's
FRQILJXUHGLQWKHSUHYLRXV location in meters then
VWHSV press [Next]”DSSHDUV(QWHU
WKHLQVWUXPHQW
VVLWHDOWLWXGHLQ
 PHWHUVWKHQFOLFNRQNext*
127(6;;;;;;;;;;;;LV
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 If SUCCESS: Calibration is
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Note
If the status box at the
bottom of the window is
not visible and you are
using a Windows 7
laptop computer, refer &(//'<1(PHUDOG6HUYLFHDQG6XSSRUW0DQXDO9HUVLRQ‡‹‡&(//'<1(PHUDOGDUHWUDGHPDUNVRI$EERWW/DERUDWRULHVLQYDULRXV
to above instructions in MXULVGLFWLRQV‡$EERWW3DUN,/‡$OOULJKWVUHVHUYHG
Windows 7 Display
Format.

 $IWHUWKUHHXQVXFFHVVIXO
&DOLEUDWLRQDWWHPSWVDQG
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 &ORVHWKHPressure Sensor
CalibrationZLQGRZ
 'LVFRQQHFWWKHFDEOHIURP
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 $IWHUDVXFFHVVIXO&DOLEUDWLRQ
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 *RWRMAINTENANCE,
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 6HOHFWVACUUMDQGYHULI\
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9DFXXP
VP-09 CELL-DYN Emerald Pressure Verification Procedure VersionBB

Inspect tools for damage, ensure calibration is not expired and replace if necessary.

At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument produces results,
ENSURE appropriate Quality Control is in specification and calibrate as necessary.

VP-09 CELL-DYN Emerald Pressure Verification Procedure

Purpose 7RFRQILUPD3UHVVXUH'HIDXOW(UURUFRQGLWLRQRQWKH&'(PHUDOG6\VWHP Module &(//'<1(PHUDOG

Materials Required 1RWRROVDUHUHTXLUHGIRUWKLVSURFHGXUH Time 2QHPLQXWH



Action Steps Reference

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 $ERYHHJ
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 VP-11 CELL-DYN Emerald 22 Optical Alignment VersionBB

Inspect tools for damage, ensure calibration is not expired and replace if necessary.

At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.

VP-11 CELL-DYN Emerald 22 Optical Alignment

FDSWLYDWHGKDUGZDUH7KH
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Materials Required Time +RXUV

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