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Problem #1: Pseudomonas 5401 Is To Be Used For Production of Single-Cell Protein For Animal Feed. The

1) Pseudomonas 5401 is being used to produce single cell protein from fuel oil. The question asks to calculate the minimum concentration of (NH4)2SO4 needed in the medium if it is the sole nitrogen source and a final cell concentration of 25 g/L is required. 2) The production of single cell protein from hexadecane is described by a chemical reaction. The question asks to determine the stoichiometric coefficients for the reaction if the respiratory quotient is 0.43. 3) The question asks to calculate the oxygen requirement in mass terms for the culture of Klebsiella aerogenes grown on glycerol using ammonia as the nitrogen source, given the biomass yield

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Hritik Lal
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582 views5 pages

Problem #1: Pseudomonas 5401 Is To Be Used For Production of Single-Cell Protein For Animal Feed. The

1) Pseudomonas 5401 is being used to produce single cell protein from fuel oil. The question asks to calculate the minimum concentration of (NH4)2SO4 needed in the medium if it is the sole nitrogen source and a final cell concentration of 25 g/L is required. 2) The production of single cell protein from hexadecane is described by a chemical reaction. The question asks to determine the stoichiometric coefficients for the reaction if the respiratory quotient is 0.43. 3) The question asks to calculate the oxygen requirement in mass terms for the culture of Klebsiella aerogenes grown on glycerol using ammonia as the nitrogen source, given the biomass yield

Uploaded by

Hritik Lal
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Problem #1

Medium formulation
Pseudomonas 5401 is to be used for production of single-cell protein for animal feed. The
substrate is fuel oil. The composition of Pseudomonas 5401 is CH 1.830 0.55 N 0.25
If the final cell concentration is 25 g 1-1, what minimum concentration of (NH4)2SO4 must be
provided in the medium if (NH4)2SO4 is the sole nitrogen source?

VIVEK R BITS Pilani, K K Birla Goa Campus


Problem #2

Production of single-cell protein from hexadecane is described by the following reaction


equation:

C16H34 +a O2 +b NH3  c CH1.66O0.27N0.20 +d CO2 + e H2O

where CH1.66O0.27N0.20 represents the biomass. If RQ = 0.43, determine the stoichiometric


coefficients.

VIVEK R BITS Pilani, K K Birla Goa Campus


Problem #3

Oxygen requirement for growth on glycerol


Klebsiella aerogenes is produced from glycerol in aerobic culture with ammonia as
nitrogen source. The biomass contains 8% ash, and 0.40 g biomass is produced for each g
of glycerol consumed, and no major metabolic products are formed. What is the oxygen
requirement for this culture in mass terms?

VIVEK R BITS Pilani, K K Birla Goa Campus


Problem #4

Cell culture using whey


Waste dairy whey is used to grow Kluyveromyces fragilis yeast in a continuous culture system operated
at 30 °C and 1 atm pressure. Medium containing 4% w/w lactose (C12H22O11) and 0.15% w/w NH3
flows into a specially designed aerated bioreactor at a rate of 200 kg h-1. The reactor is
compartmentalised to facilitate gravity settling of the yeast; a suspension containing concentrated cells is
drawn off continuously from the bottom of the reactor at a rate of 40 kg h-1, while an aqueous waste
stream containing 0.5 kg cells per 100 kg leaves from the top. All of the lactose provided is utilized by
the culture. The biomass yield from lactose is known from preliminary experiments to be 0.25 g g-1. The
composition of the K. fragilis biomass is determined by elemental analysis to be
CH1.63O0.54N0.16 + 7.5% ash.
(a) What is the RQ for this culture?
(b) It is proposed to supply the reactor with air at a maximum flow rate of 305 litres min-1.
Will this provide sufficient oxygen? If not, what minimum level of oxygen enrichment
of the air is needed if the total gas flow rate is 305 litres min-1 ?
(c) What is the concentration of residual NH3 in the aqueous waste stream?
(d) What is the concentration of cells in the cell concentrate?
VIVEK R BITS Pilani, K K Birla Goa Campus
Top stream (200 -40) = 160
4 % w/w lactose = 8 kg/h kg/h
0.15 % w/w NH3 = 0.3 kg/h Cells = 0.5 kg/100 kg stream
Cell flow rate = 0.8 kg/h
Input media = 200 kg/h Kluyveromyces fragilis

Bottom stream flow rate= 40 kg/h


Air flowrate = 305 L/min Concentrated cells
VIVEK R BITS Pilani, K K Birla Goa Campus

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