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    Scanning Probe Acceleration Microscopy: Towards Real Time Reconstruction of Tip-Sample Forces in Tapping Mode AFM

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    LIAKMAN
    This document summarizes research towards developing scanning probe acceleration microscopy (SPAM) to reconstruct tip-sample forces in tapping mode atomic force microscopy (AFM) in real time. SPAM analyzes the cantilever trajectory during tapping using a sliding window Fourier transform combined with a harmonic comb filter. This approach could allow direct measurement of tip-sample interaction forces with high signal-to-noise ratio. Preliminary experiments demonstrate SPAM can image soft materials like lipid bilayers in liquid environments.

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    Scanning Probe Acceleration Microscopy: Towards Real Time Reconstruction of Tip-Sample Forces in Tapping Mode AFM

    Uploaded by

    LIAKMAN
    63 views40 pages
    This document summarizes research towards developing scanning probe acceleration microscopy (SPAM) to reconstruct tip-sample forces in tapping mode atomic force microscopy (AFM) in real time. SPAM analyzes the cantilever trajectory during tapping using a sliding window Fourier transform combined with a harmonic comb filter. This approach could allow direct measurement of tip-sample interaction forces with high signal-to-noise ratio. Preliminary experiments demonstrate SPAM can image soft materials like lipid bilayers in liquid environments.

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    This document summarizes research towards developing scanning probe acceleration microscopy (SPAM) to reconstruct tip-sample forces in tapping mode atomic force microscopy (AFM) in real time. SPAM analyzes the cantilever trajectory during tapping using a sliding window Fourier transform combined with a harmonic comb filter. This approach could allow direct measurement of tip-sample interaction forces with high signal-to-noise ratio. Preliminary experiments demonstrate SPAM can image soft materials like lipid bilayers in liquid environments.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    63 views40 pages

    Scanning Probe Acceleration Microscopy: Towards Real Time Reconstruction of Tip-Sample Forces in Tapping Mode AFM

    Uploaded by

    LIAKMAN
    This document summarizes research towards developing scanning probe acceleration microscopy (SPAM) to reconstruct tip-sample forces in tapping mode atomic force microscopy (AFM) in real time. SPAM analyzes the cantilever trajectory during tapping using a sliding window Fourier transform combined with a harmonic comb filter. This approach could allow direct measurement of tip-sample interaction forces with high signal-to-noise ratio. Preliminary experiments demonstrate SPAM can image soft materials like lipid bilayers in liquid environments.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 40

    Scanning Probe Acceleration Microscopy:

    Towards Real Time Reconstruction of Tip-Sample Forces in Tapping Mode AFM

    Tomek Kowalewski

    Department of Chemistry

    Carnegie Mellon University

    PNAS, (2006), 103, 4813-4818


    Appl.Phys.Lett., (2005), 87, 163120/1-163120/3
    Acknowledgements:

    Justin Legleiter

    Brian Cusick

    Matthew Park

    Funding:
    NSF: CTS-0304568, DMR-9974457
    E = 109 Pa
    E = 108 Pa
    E = 107 Pa
    Plotting tapping force vs. cantilever deflection reveals that each tapping
    event resembles closely the contact mode force curve.
    The slope of these “mini force curves” depends in a familiar way on teh
    sample stiffness and dictates the width of the tapping pulse
    Towards direct reconstruction of tip-sample force from
    cantilever trajectory
    Scanning Probe Acceleration Microscopy

    z + bz& + k [ z − Do − a0 sin(2π f op t )] = Ftip − sample


    meff &&

    Cantilever
    acceleration
    1
    z=
    && [ Ftip − sample − bz& − kz + k ( Do − a0 sin(2π f op t ))]
    meff
    These terms vary as exp( i ωop t)
    Tapping
    force pulse

    PNAS, (2006), 103, 4813-4818


    S:N = 105
    S:N = 104
    S:N = 103
    S:N = 103
    Harmonic Comb Filtering

    ⎡ N
    ⎤
    yrec (t ) = ℑ ⎢ y (ω )∑ δ (ω − kωoper ) ⎥
    −1

    ⎣ k =1 ⎦

    PNAS, (2006), 103, 4813-4818


    −1 ⎡ ⎤
    N
    yrec (t ) = ℑ ⎢
    ⎣
    y (ω ) ∑
    k =1
    δ (ω − k ω oper ) ⎥
    ⎦
    Key To Modeling “Fluid Tapping” : LOW Q!

    mω o
    Q=
    b
    Ao
    Q=
    ao

    z + bz& + k [ z − D0 − a0 sin(ω0t )] = Fext


    meff &&
    where
    Fext = − HRtip / 6 z 2
    for z > aDMT

    Fext = 4 /(3πκ eff ) R tip ( a DMT − z ) 3 / 2 − HR tip / 6 a DMT


    2
    for z < aDMT
    Simulated fluid tapping cantilever position

    Appl.Phys.Lett., (2005), 87, 163120/1-163120/3


    Fluid tapping cantilever trajectories
    Simulated fluid tapping cantilever position

    Appl.Phys.Lett., (2005), 87, 163120/1-163120/3


    Recall that optical lever measures cantilever deflection –
    not position!

    y = z − D0 + a0 sin(ω t )
    Appl.Phys.Lett., (2005), 87, 163120/1-163120/3
    Fluid Tapping Force-Curve
    Experiment Simulation

    Appl.Phys.Lett., (2005), 87, 163120/1-163120/3


    SPAM under water

    PNAS, (2006), 103, 4813-4818


    PNAS, (2006), 103, 4813-4818
    1 ⎡ 1 ⎤
    &y& = ⎢ Fext − b &
    y − ky + m eff ω 2
    a 0 [sin(ω t ) + cos(ω t )]⎥
    meff ⎣ Q ⎦
    PNAS, (2006), 103, 4813-4818
    1 ⎡ 1 ⎤
    &y& = ⎢ Fext − b &
    y − ky + m eff ω 2
    a 0 [sin(ω t ) + cos(ω t )]⎥
    meff ⎣ Q ⎦
    1 ⎡ 1 ⎤
    &y& = ⎢ Fext − b &
    y − ky + m eff ω 2
    a 0 [sin(ω t ) + cos(ω t )]⎥
    meff ⎣ Q ⎦
    1 ⎡ 1 ⎤
    &y& = ⎢ Fext − b &
    y − ky + m eff ω 2
    a 0 [sin(ω t ) + cos(ω t )]⎥
    meff ⎣ Q ⎦
    The effect of noise
    Harmonic Comb Filter

    ⎡ N
    ⎤
    yrec (t ) = ℑ ⎢ y (ω )∑ δ (ω − kωoper ) ⎥
    −1

    ⎣ k =1 ⎦
    Sliding window Fourier transform

    PNAS, (2006), 103, 4813-4818


    Sliding window Fourier transform

    PNAS, (2006), 103, 4813-4818


    Sliding window Fourier transform
    Calibration curve

    PNAS, (2006), 103, 4813-4818


    Total brain lipid extract bilayers
    PNAS, (2006), 103, 4813-4818
    PNAS, (2006), 103, 4813-4818
    Conclusions:

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