Cambridge International General Certificate of Secondary Education
Cambridge International General Certificate of Secondary Education
Cambridge International General Certificate of Secondary Education
BIOLOGY 0610/53
Paper 5 Practical Test October/November 2019
1 hour 15 minutes
Candidates answer on the Question Paper.
Additional Materials: As listed in the Confidential Instructions.
Write your centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use an HB pencil for any diagrams or graphs.
Do not use staples, paper clips, glue or correction fluid.
DO NOT WRITE IN ANY BARCODES.
At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.
Total
This syllabus is regulated for use in England, Wales and Northern Ireland as a Cambridge International Level 1/Level 2 Certificate.
DC (RW/CB) 173490/3
© UCLES 2019 [Turn over
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You are going to investigate the effect of light on photosynthesis in a chloroplast suspension.
The blue dye DCPIP can be used to investigate photosynthesis. During photosynthesis the dark
blue colour of the DCPIP changes and eventually disappears so that it becomes colourless.
Read all the instructions but DO NOT CARRY THEM OUT until you have drawn a table for
your results in the space provided in 1(a)(i).
You should wear the gloves and eye protection provided during the practical work in question 1.
Step 1 You are provided with eight green leaves. Tear the leaves into small pieces and place
them into the mortar (bowl).
Step 2 Pour all of the ice‑cold solution from the beaker labelled isolation medium into the
mortar (bowl).
Step 3 Use the pestle (or spoon) to grind the leaves in the isolation medium for three minutes.
Step 4 Place three layers of muslin into the funnel. Put the funnel into the large test‑tube
labelled C.
large test-tube
Fig. 1.1
Step 5 Carefully hold the edges of the muslin and pour the ground leaves and isolation medium
mixture from the mortar (bowl) into the muslin‑lined funnel. When most of the liquid has
filtered into the large test‑tube, as shown in Fig. 1.1, place the muslin and funnel in the
waste container.
Step 6 Raise your hand when you are ready for ice‑cold water to be added to the water‑bath.
Step 7 Wrap aluminium foil around one empty test‑tube to exclude light. Leave the opening
at the top of the test‑tube uncovered. Do not wrap the second test‑tube. Place both
test‑tubes into the test‑tube rack.
Step 8 Use the plastic pipette to add 2 cm3 of filtrate C to each of the empty test‑tubes.
Step 9 Use a 5 cm3 syringe to add 5 cm3 of DCPIP solution to each of the two test‑tubes. Place
a stopper in both test‑tubes. Stand both test‑tubes in the water‑bath and position the
water‑bath near a bright light source or lamp.
Step 11 After five minutes observe the colour in the uncovered test‑tube and record it in your
table in 1(a)(i).
Step 12 Remove the aluminium foil from the other test‑tube. Immediately observe the colour of
the liquid in the test‑tube. Record this in your table in 1(a)(i).
[3]
(ii) Identify the variable that was changed (independent variable) and the variable that was
measured (dependent variable) in this investigation.
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[2]
(iii) State two variables that have been kept constant in this investigation.
1 ........................................................................................................................................
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2 ........................................................................................................................................
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[2]
© UCLES 2019 0610/53/O/N/19 [Turn over
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error 1 ................................................................................................................................
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error 2 ................................................................................................................................
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[2]
(iii) Suggest an improvement for one of the errors you have identified in 1(b)(ii).
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(c) A student wanted to investigate the effect of temperature on the rate of photosynthesis in a
chloroplast suspension.
Describe how the student could carry out this investigation using DCPIP.
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[Total: 18]
stoma
guard cell
epidermal cell
N
magnification ×400
Fig. 2.1
(a) (i) Make a large drawing of Fig. 2.1. Do not label your drawing.
[4]
Calculate the actual width of the guard cells and the stoma indicated by line MN in
Fig. 2.1. Use the equation and information in Fig. 2.1.
..........................................................mm
[3]
Strips of epidermis from leaves were placed in different concentrations of sucrose solution for
one hour.
The student measured the width of 10 stomatal openings from the epidermis in each sucrose
solution.
Table 2.1
0 5 6 7 5 5 6 7 5 7 6 5.9
5 3 4 3 3 4 3 2 3 3 2
20 1 2 1 2 1 1 5 1 2 1 1.7
(i) Circle one measurement in Table 2.1 that could be anomalous. [1]
(ii) Calculate the average width of stomatal opening in the 5% sucrose solution.
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[2]
Scientists investigated the number of stomata open, in pineapple plants, at different times of
day.
Table 2.2
average number of
time of day
stomata open per mm2
00:00 77
04:00 61
08:00 22
12:00 4
16:00 10
20:00 51
(i) Calculate the percentage change in the average number of open stomata per mm2
between 16:00 hours and 20:00 hours.
.............................................................%
[2]
(ii) Plot a bar chart on the grid to show the data in Table 2.2.
[3]
(iii) Describe, using your graph, how the average number of open stomata per mm2 changes
throughout the day.
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(d) When fruit ripens the starch contained within it is converted into reducing sugars.
(i) State the name of the substance that is used to test for the presence of starch.
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(ii) Describe how you could safely show a reducing sugar was present in a sample of
pineapple fruit juice.
procedure ..........................................................................................................................
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safety .................................................................................................................................
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[Total: 22]
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