CY009

English
CYANSmart
User Manual

For a clear and precise diagnose

ISO 13485-2016
www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
Layout 20181024
INDEX
1  INTRODUCTION .................................................................................................................................. 4 
1.1  PRINCIPLE OF MEASUREMENT ...........................................................................................................................4 
1.2  INTENDED USE ...................................................................................................................................................5 
1.3  SYSTEM DESCRIPTION........................................................................................................................................5 
1.3.1 Thermal carbon printer 6 
1.3.2 Touch screen 6 
1.3.3 Optical assembly 7 
1.3.4 Incubator block 8 
1.3.5 Hydraulic and aspiration system 8 
1.3.6 Rear panel 8 
1.4  TECHNICAL & OPERATIVE SPECIFICATIONS .......................................................................................................9 
1.5  LIMITATIONS OF USE....................................................................................................................................... 10 
1.6  REGULATORY COMPLIANCES ......................................................................................................................... 10 
2  INSTALLATION ................................................................................................................................. 11 
2.1  TRANSPORT AND STORAGE ............................................................................................................................ 11 
2.2  UNPACKING .................................................................................................................................................... 11 
2.3  INSTALLATION................................................................................................................................................. 13 
2.3.1 Locations specifications 13 
2.3.2 Power supply connection 13 
2.3.3 Install the peristaltic pump tube 14 
2.3.4 Install the flowcell, aspiration tube and aspiration button 15 
2.3.5 Install the waste tube and waste bottle 17 
2.3.6 Switch on the analyzer 17 
2.3.7 Install or change the print paper 17 
2.3.8 Verify the instrument 18 
2.3.9 Adaptation of the system to the end customer 18 
3  SOFTWARE DESCRIPTION ............................................................................................................. 21 
3.1  SYSTEM START – “SELF-TEST” ....................................................................................................................... 21 
3.2  MAIN MENU .................................................................................................................................................... 21 
3.3  PERFORM TEST MENU ..................................................................................................................................... 22 
3.3.1 AD auto zero (AD Value) 24 
3.3.2 Test Blank 24 
3.3.3 Test standard/calibrator 25 
3.3.4 Test quality control N/P 26 
3.3.5 Sample measurement 27 
3.3.6 End of test 28 
3.3.7 Specific method instructions and formulas 28 
3.4  METHODS ....................................................................................................................................................... 33 
3.4.1 Program Add 33 
3.4.2 Program Modify 36 
3.4.3 Program Delete 37 
3.4.4 Program List 37 
3.5  ARCHIVES ........................................................................................................................................................ 38 
3.5.1 Result List 38 
3.5.2 Delete all 40 
3.5.3 Data Synchronization 40 
3.5.4 Print report 40 
3.5.5 Control manage 40 
3.6  TUBE WASH ..................................................................................................................................................... 42 
3.7  PRINTER FEED ................................................................................................................................................. 42 

CYANSmart user manual, Version 20160902_(5.1) Page 1

 3.8  PUMP CALIBRATION........................................................................................................................................ 43 
3.9  AD ZERO ........................................................................................................................................................ 44 
3.10  FILTER TEST..................................................................................................................................................... 46 
3.11  SYSTEM SETUP ................................................................................................................................................ 46 
3.11.1  Temp Display 47 
3.11.2  Printer Setup 47 
3.11.3  Standby Setup 49 
3.11.4  Date Format 50 
3.11.5  Date and Time 50 
3.11.6  System Info 50 
3.11.7  Hospital Setup 50 
3.11.8  Language Setup 50 
3.11.9  Screen brightness 50 
3.12  RESERVED SETTING......................................................................................................................................... 50 
3.12.1  Temp calibration 50 
3.12.2  Filter and cuvette 50 
3.12.3  Aspirate format 50 
3.12.4  Heading setup 51 
4  DAILY ROUTINE ............................................................................................................................... 51 
4.1  PREPARATIVE WORK ....................................................................................................................................... 51 
4.1.1 Preparation of the analyzer 51 
4.1.2 Perform an AD zero 51 
4.1.3 Preparation of the reagent 51 
4.1.4 Preparation of the samples 51 
4.2  START SAMPLE ANALYSIS ............................................................................................................................... 51 
4.2.1 AD Value 51 
4.2.2 Blank measurement 52 
4.2.3 Standard/calibrator measurement 52 
4.2.4 Quality control measurement 52 
4.2.5 Sample measurement 53 
4.3  END OF TEST ................................................................................................................................................... 53 
4.4  EVALUATE THE RESULTS ................................................................................................................................. 53 
4.5  SHUT DOWN - END OF THE DAY .................................................................................................................... 53 
4.6  WEEKLY........................................................................................................................................................... 53 
5  MAINTENANCE ................................................................................................................................ 55 
5.1  PUNCTUAL MAINTENANCE ............................................................................................................................ 55 
5.2  ROUTINE MAINTENANCE ................................................................................................................................ 55 
5.2.1 Washing 55 
5.2.2 Cleaning 56 
5.2.3 Verification 56 
5.3  REPLACEMENT OF SPECIFIC PARTS ................................................................................................................. 57 
5.3.1 External cleaning 57 
5.3.2 Internal washing 57 
5.3.3 Replacement schedule 57 
5.3.4 Replacement of peristaltic pump tube 57 
6  CONNECTION SOFTWARE ............................................................................................................. 59 
6.1  MINIMUM REQUIREMENTS FOR THE PC ........................................................................................................ 59 
6.2  SOFTWARE INSTALLATION.............................................................................................................................. 59 
6.2.1 Adjustment of the settings on your computer 59 
6.2.2 Installation of the CYANSmart software on your computer 61 
6.3  LAUNCH THE SOFTWARE ................................................................................................................................ 62 
6.4  DESCRIPTION OF THE SOFTWARE ................................................................................................................... 63 
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 6.4.1 Home screen 63 
6.4.2 Upload results from the CYANSmart to the software 63 
6.4.3 Manage patient results 64 
6.4.4 Print patient reports 66 
6.4.5 Methods 67 
6.4.6 Methods update 69 
7  TROUBLESHOOTING ....................................................................................................................... 71 
7.1  OPERATIONAL................................................................................................................................................. 71 
7.2  TECHNICAL...................................................................................................................................................... 73 
8  SAFETY INFORMATION .................................................................................................................. 75 
MAINTENANCE FORM
MAINTENANCE INSTRUCTIONS
REPAIR FORM
INSTALLATION FORM
CONSUMABLES

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1 INTRODUCTION
The CYANSmart is an easy to operate semi-automatic biochemical analyzer. This new in vitro diagnostic
instrument is designed to have powerful features in a compact, independent unit.

Cost savings
 Minimum reaction volume of 500 μl results in a decreased cost per test
 Limited consumables required
 Extra consumables included at delivery (two fuses, five paper rolls a spare lamp and pump tube)
 Integrated incubator, printer, pump, flowcell
 Long life lamp, even further extended by power saving / standby mode
 Robustness of the analyzer with minimal daily maintenance
 Analyzer guides the user and thereby reduces mistakes

Flexibility
 On board printer for automatic or on-request printing
 Seven wavelengths (340, 405, 492, 510, 546, 578 and 620 nm filters) cover all clinical diagnostic
applications
 Five calculation methods (absorbance, end point, two-point, kinetic, bichromatic) and three
calibration methods (factor, calibrator and multi-point calibration) provide plenty processing
options with one system
 Three blank options (sample blank, reagent blank and water) provide background correction of the
reagent and sample coloration

Convenience
 All Cypress Diagnostics biochemistry reagents are pre-programmed at delivery
 180 open channels are programmable to allow for unrestricted testing
 Memory for up to 6000 sample results
 Large font size, pleases the eye and reduced the effort
 LCD touch screen with virtual alphanumeric keyboard
 Multilingual capability
 Minimal daily maintenance

1.1 PRINCIPLE OF MEASUREMENT

The CYANSmart is a semi-automated biochemical analyzer. Samples are prepared manually outside the
instrument and after an incubation period (in- or outside the instrument) the samples are measured in the
flowcell and the analyzer calculates the result.
A halogen lamp emits light which passes through a filter selecting the required wavelength. The light then
passes through the sample in the flowcell. The sample will transmit or reflect fractions of the light. The
detector will measure how much light was reflected from/transmitted through the sample. This
measurement is then amplified and converted to a digital number for further calculations. These
calculations can be performed because there is a relationship between how much light is
reflected/transmitted and the concentration of the substances in a sample.

Lamp Filter Flowcell Photodiode detector

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1.2 INTENDED USE
(Semi-) automatic biochemistry analyzers are medical laboratory instruments, widely applied in hospitals
and research institutes. They are designed for the quantitative determination of different biochemical
substances and other characteristics in a number of biological samples. These measured properties of blood
and other fluids may be useful in the diagnosis of disease.
The type of in vitro diagnostic test that can be performed on this analyzer include enzyme levels (such as
many of the liver function tests), ion levels (e.g. sodium and potassium), and other tell-tale chemicals (such
as glucose, serum albumin, or creatinine). Enzymes may be measured by the rate they change one colored
substance to another; in these tests, the results for enzymes are given as an activity, not as a concentration
of the enzyme. Other tests use colorimetric changes to determine the concentration of the chemical in
question. Turbidity may also be measured.
The automation of laboratory testing does not remove the need for human expertise (results must still be
evaluated by medical technologists and other qualified clinical laboratory professionals), but it does
minimize the potential human errors, resulting in higher precision and accuracy. Furthermore, it reduces
sample and reagent volumes and increases the speed of the testing compared to measurement with a
spectrophotometer.
NOTE:
The CYANSmart is a system that allows configuration with different reagents selected by the customer in
order to fit his needs. Upon delivery, the instrument is configured for the validated biochemistry kits of
Cypress Diagnostics. In order to assure the analytical performances of the system “instrument + reagents”,
we strongly recommend only to use reagents, controls and calibrators validated on the CYANSmart.

1.3 SYSTEM DESCRIPTION

The CYANSmart is a microcomputer-based independent unit, controlling several systems: built-in thermal
printer, touch screen, optical detection system with flowcell, incubator and aspiration system.

1. Thermal printer
2. Touch screen
① 3. Optical system
4. Incubator block
5. Peristaltic pump
8 6. Aspiration “Push” button
④
③ 7. Aspiration inlet
⑤ 8. Rear panel

After a certain time of inactivity (programmable in the system setup), the instrument will go into a power-
saving “Standby” mode:
 The touch screen is black.
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  The lamp is supplied with low voltage power (3.0 V).
 All other parts are kept ready for urgency samples: incubator and flowcell are maintained at 37°C.
Upon touching the screen:
 The touch screen is lit up.
 The lamp is supplied with regular voltage power (6.0 V).

1.3.1 Thermal carbon printer

The CYANSmart is equipped with a built-in thermal printer. The text or graph is
printed on coated thermo-chromic paper. This paper is selectively heated when
passing over the thermal printing head, and thereby the coating on these places
turns black, producing an image. One of the big advantages of a thermal printer
over a normal printer is that it does not require expensive consumables like a
toner.

1.3.2 Touch screen

The CYANSmart is equipped with a user friendly, touch-sensitive crystal display. A finger or smaller blunt
object (stylus pen) should be used to operate the touch screen. If the operator gently touches the LCD, the
analyzer will recognize the position where the screen was pressed; the software will activate the
corresponding function/menu/button.
Upon touching a specific function/key/menu, this button will become darker blue (indicating its activation)
and the CYANSmart will make a beeping sound.

When data needs to be entered, a keyboard will appear. The keyboard will be adapted to the data that can
be entered in that specific area:
1. A complete alphanumerical keyboard. Press the CAP button until it becomes orange to enter capital
letters (right panel). To confirm an insertion and return to the previous menu, press “OK”

2. The second mode is shown when only numbers are required:

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! ATTENTION POINT!
The screen can only react to one touch point at the same time. If something else is touching or pressing the
screen (on an empty position), it will not respond to your touch!

1.3.3 Optical assembly

① Lamp
② Filter wheel with filters
④ ③ Motor for filter wheel
⑦ ④ Flowcell
①
② ⑤ Temperature sensor
⑥ Peltier element
③ ⑦ Photodetector board
⑤
⑥

The optical system is the functional key compound of the instrument. It contains the light source, filters for
wavelength selection, flowcell with heating mechanism and a PC board that contains the photodiode
detector. This pre-amplifier board also converts the light detection from an analog to a digital signal for
further processing on the main board.
The 6V, 10W halogen lamp is installed on a lamp holder. The normal life span of the lamp is 2000 hours (= 1
year). The CYANSmart has a power saving standby mode. After a specified time of inactivity, the instrument
will enter this standby mode and this will diminish the voltage to the lamp from 6V to 3V, thereby increasing
the life time of the lamp.
The filter wheel has 10 positions: one dark filter, 7 interferential filters (340 nm, 405 nm, 492 nm, 510 nm,
546 nm, 576 nm and 620 nm) and 2 free positions. The correct filter is aligned in the light pad through a
cooperation between the filter motor and the filter home sensor. The wavelength accuracy is ± 2 nm.
The light then passes the flowcell, which has a light path of 10 mm. The flowcell is heated with a Peltier
element to 25°C, 30°C or 37°C and the heating is regulated by temperature sensor.
Ultimately, the light is detected by a silicon photodiode on the pre-amplifier board. This photodetector has
a range of 300-900 nm.
Absorbance measuring range:
0.0~3.500 OD
Absorbance linearity:
a) absorbance is 0.200 ~ ≤0.500, the bias is within ±5%
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 b) absorbance is >0.500 ~ ≤1.000, the bias is within ±4%
c) absorbance is >1.000 ~ ≤1.800, the bias is within ±2%
d) absorbance is >1.800, the bias is within ±5%
Stability:
At 340nm, in 20 min, the absorbance drift of distilled water is ≤0.005.
Repeatability:
The variable coefficient of repeatability measurement should be CV ≤1.0%
Carry-over:
If the total reaction fluid is 1.0 ml, the carry-over of neighboring samples is ≤1.0%

1.3.4 Incubator block

The incubator has a fixed set temperature of 37±1°C. There are 20 spaces for reaction tubes up to 14 mm
diameter. After start-up of the instrument, the instrument requires 15 - 30 minutes to reach the
programmed temperature, the temperature will remain constant until the instrument is turned off.

1.3.5 Hydraulic and aspiration system

The peristaltic pump is situated on the left side of the instrument. It can be activated by:
- The ‘Tube wash’ button on the touch screen.
- The aspiration switch by pushing the aspiration button.
Upon activation of the peristaltic pump, a quantitative volume is aspirated in the hydraulic system. This is a
continuous line consisting of various tubes, connectors, the flowcell, and ending in the waste bottle.

Inlet fastening
screw

Waste bottle Rear panel Peristaltic pump Flowcell Aspiration Aspiration

switch button
Tubes
CY004/9-S26 Plastic aspiration tube
CY004/9-S27 Metal aspiration tube
CY004/9-S41 Tube from flowcell to pump
CY009-S25 Pump tube
CY004/9-S40 Tube from pump to waste connector
CY004/9-S28 Waste tube
Connectors
CY009-S30 Connector of pump tube (2 pcs)

CY009-S29 Connector of waste tube

1.3.6 Rear panel

Below you can find a picture of the rear panel of the CYANSmart.

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 ②

③ ④
⑦

⑤ ⑥
① Fan for internal cooling (80 x 80)
② Back label with serial number
③Power plug connector with fuses 2 x T2AL250V
④ Power switch
⑤ RS232 connection
⑥ USB port connection
⑦ Connector of waste tube
Symbol Explanation
Attention:
Read the instructions in the user manual before use
Biological hazard:
Carefully manipulate all the consumables and the waste produced during the analysis
routines. Use appropriate protective garment. Disposal of waste must be done in
compliance with applicable regulation. It is recommended to periodically check the level
in the waste container, in order to avoid overflow.

1.4 TECHNICAL & OPERATIVE SPECIFICATIONS

Optical system
Flowcell 32 μl, 10 mm light path
Min. reaction volume 500 μl per test
Light source Halogen lamp 6V / 10W
Photo detector Silicon based (range 300 – 900 nm)
Measurement range 0,000 – 3,500 Abs
Wavelength 340 – 620 nm
Wavelength selection Automatic via 7 interference filters: 340nm, 405nm, 492nm, 510 nm,
546nm, 578nm, 620nm
Flowcell
 25°C, 30°C, 37°C, ambient temperature (R.T.)
 Peltier element
 Thermostatic control: PID controlled, ± 0.5°C
Incubator
 20 places with 14 mm diameter
 37°C
 Resistance wire
 Thermostatic control: PID controlled, ± 1°C
Display
 Back-illuminated LCD
 Touch screen calibration possible
 800 x 480 pixels (screen size: 7.0 Inch, 155.3 x 88 mm)
Printer
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  Automatic or on-demand printing
 Built-in thermal printer
 24 characters per line
 Prints graphs
Software
Data storage 180 test methods & 6000 sample test results
Methods Cypress methods pre-programmed at delivery
Languages English, French, Spanish
Quality control feature 2 controls programmable
Separate QC result menu with (printable) graphs
Calculation methods Absorbance, end point, two-point, kinetic, bichromatic
Calibration methods Factor, calibrator and multi-point calibration
Blank options Sample blank, reagent blank and distilled water
Incubation time 0 – 999 seconds
Reading time 3 – 999 seconds
Power supply
 AC 230 V ± 10 %, 50 Hz ± 2 % or AC 110 V ± 10 %, 60 Hz ± 2 %
 Automatic voltage and hertz switch
 Grounding required
 2 fuses T2AL250V
 Power: 200VA, reduced to 140 VA in standby mode
 Power save / Standby mode
Weight and dimensions
 Instrument: 9 kg, 435 x 415 x 200 mm (LxWxH)
 Packed in box: 11.5 kg, 520 x 430 x 260 mm (LxWxH)
Environmental requirements
 Ambient temperature: 15°C – 30°C
 Storage temperature: 5-50°C
 Relative humidity: 30 % - 70%
 Air pressure: 860 hPa – 1060 hPa

1.5 LIMITATIONS OF USE

After turning on the instrument, pay attention not to spill liquids on the surface around the instrument.
Keep the instrument away from children.
1.6 REGULATORY COMPLIANCES
The CYANSmart complies with European Directive 97/79/CE for In Vitro diagnostic.

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2 INSTALLATION

2.1 TRANSPORT AND STORAGE

The packing has been expressly studied and designed to insure maximum protection of the contents during
shipping and handling. It is therefore extremely important that the box is carefully examined upon delivery
in order to assure the integrity. All visible external damage (for example: holes, dents, rips or tears, water
marks, etc.) must be noted on the delivery note. Please make photos of the damage. This will simplify
matters in the event of any future claims for damages.
NOTE:
Once the carrier has taken possession of the system for transportation from the factory, the carrier takes
total responsibility until delivery. All claims for damage due to transportation must be filed against the
carrier as soon as these damages are noticed.
The CYANSmart carton box should be stored in an environment with a temperature range of 5 – 50 °C,
relative humidity not more than 80 %, well ventilated and indoors. Storage should be away from toxic,
harmful and corrosive substances. Make sure the box is in the upright position (arrows on box).

2.2 UNPACKING
Open the box and carefully take out the analyzer and accessories.
1. Makes sure the SN (on the back label) is in accordance with the delivery note/packing list.

2. Check that all the items of the following list are undamaged and included in the carton box.
Code Description Quantity
CY009 CYANSmart Analyzer 1
- User manual including application sheets 1
- CD with PC software (from P/D 2016/09 on) 1
CY004/9-S28 Waste tube 1
CY004/9-S44 Waste bottle 1
CY004/9-S22 Aspiration button 1
CY004/9-S49 Power cord 1
CY004/9-S48 Fuse T2AL250V 2
CY009-S11 Lamp with frame, cable and connector 1
CY009-S25 Pump tube 1
CY001-S01 Thermal print paper 5
CY004/9-S43 Dust cover 1
CY009-S56 USB cable (from P/D 2016/04 on) 1

If the instrument is damaged or items are missing, contact your local distributor/importer immediately for
assistance. Make sure to document with photos.

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! WARNING! Do NOT discard the carton box or protection material! If you need to ship the instrument
(for example for service), be sure to use the original packing materials. Other forms of commercially available
packing are not recommended and can void the warranty.

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2.3 INSTALLATION
The CYANSmart is an easy to operate instrument with integrated user-friendly software. No additional
computers or instruments are needed. In order to fully guarantee the performance of the CYANSmart, it is
extremely important that it is correctly installed.
If the installation, usage and maintenance directions, given in this manual, are not followed correctly and/or
safety indications are not respected, Cypress Diagnostics cannot guarantee correct functioning of the
instrument. Misuse can compromise the safety of the operator and his surroundings and will void the
warranty. Consumables are not included in the warranty.
Procedure
1. Verify the locations requirements
2. Connect power cord to a suited power supply
3. Install the peristaltic pump
4. Install the waste tube and the waste bottle
5. Install the flowcell, aspiration button and aspiration tube
6. Switch on the instrument
7. Install printing paper
8. Verify the instrument
9. Adaptation of the system to the end customer

2.3.1 Locations specifications

Please take note of the following conditions required for the location of the installation:
 Clean, dust-free environment.
 Not in direct sunlight, this could affect the operating temperature and the quantity of light
measured by the instrument.
 Avoid draughts, and do not place under/up/ besides air conditioning or heat sources.
 Away from other appliances that generate electrical noise or magnetic fields (e.g. radiological
instruments, X-ray, radio…).
 Placed on a stable, horizontal surface, free from vibrations.
 Leave 10 cm around and at the back of the instrument for air circulation and cooling.
 Room temperature between 15 – 30 °C.
 Humidity between 30 – 70 %.
 Atmospheric pressure between 860 hPa – 1060 hPa.

2.3.2 Power supply connection

Procedure:
1. Connect the power cord to a suited and grounded AC wall outlet, preferably one that is not shared
with other electric appliances and with low fluctuation of line voltage.
2. If the AC wall outlet is not properly grounded, please connect a ground or earth wire to the bottom
plate of the analyzer.
VOLTAGE AC 220V ± 10%, 50Hz ± 2%
or AC 110V ± 10%, 60Hz ± 2%
FUSES 2x T2AL250V
The instrument can work at 110 or 220 V. The correct voltage setting is automatically detected and selected
by the apparatus. Below the power connection two fuses (T2AL250V) are present. They prevent damage to
the instrument due to overcurrent.
 After turning off the analyzer, you need to wait at least 60 seconds before turning it on again!
Otherwise, the fuses will blow because of the residual current.
CYANSmart user manual, Version 20160902_(5.1) Page 13
  ! Fire hazard. For continued protection replace only with the same type and rating of the fuses!
Always disconnect power cord before replacing the fuses!
 In order to ensure proper instrument functioning, the manufacturer strongly advises to use a
stable tension supply outlet (±10 %). If this cannot be guaranteed, use of the following
supplementary devices is necessary:
1) Electronic Stabilizer
This is used to stabilize the electric voltage in the laboratory. Any stabilizer, currently
available on the market, with a power potential of at least 0.5 KW can be used.
2) No Break module UPS - (Uninterrupted Power Supply). This module provides two
important functions:
 Stabilizes the main-line power.
 Supplies current to the instrument in case of a main-line power failure.
NOTE:
In case of an electric shock, the parameters saved on the motherboard can be modified or deleted. Please
ask the technician of your distributor to check and if necessary readjust the programming methods, the
pump calibration, the temperature calibration and the settings of filters and flow cell.

2.3.3 Install the peristaltic pump tube

Upon receiving your CYANSmart, the pump tube will be loose. To make the CYANSmart operational; the
pump tube needs to be connected/fastened.
1. Lift the handle to open the door on the side to the peristaltic pump.
2. Place the tube (1) around the peristaltic pump rotor (2).
3. Make sure to insert the tubes in the correct direction. The tube coming from the flowcell (4) towards the
front and the tube going to the waste connector (5) towards the back.
4. Then close the hinged lock (3). Make sure it is completely clicked on.
5. To verify the correct fastening of the peristaltic pump tube, the user can manually turn the wheel of the
peristaltic motor (correct direction!). If the wheel can be turned smoothly, the tubing is placed correctly,
if not, check the attention points.
! This manual turning can/should only be performed when the instrument is OFF! When the instrument
is ON, the motor movements are blocked and manually turning the rotor can damage the mechanism!
③

①
②

⑤ ④

OUT IN
To waste connector From flowcell
! ATTENTION POINTS!
1. Make sure the pump tube is placed in the middle of the white rollers of the rotor.
2. Verify that the tube is completely inserted into the holders.
3. Make sure the pump tube connectors are completely inserted into the pump tube.
4. Make sure the tube (4) and (5), are inserted in the wholes and not squashed or bent by the case.

CYANSmart user manual, Version 20160902_(5.1) Page 14

 √
X √
X

2.3.4 Install the flowcell, aspiration tube and aspiration button

1. Open the optical cover.
2. If the flowcell (1) is wrapped (protection during transport), unpack it, insert it in the reading well and
secure its position. Check the attention points.
3. Insert the metal part (2) of the aspiration inlet in the designated support and secure the position by
tightening the screw (3).
4. Under the aspiration tube, a switch/lever (4) is present. Place the aspiration button (5) over this lever.
5. Close the optical cover.

⑦
③
① ⑥
②
②
③ ④ ⑤

! ATTENTION POINTS!
1. Insert the flowcell in the correct direction! The light has to be able to pass through the windows of the
flowcell, thus the windows need to be facing to the front and to the back.
2. The inlet pipe (6) is thin, connected to the aspiration tube and should be facing forward. The outlet
tube (7) is thick, connected to the tube going to the peristaltic pump and should be facing to the back.
3. Insert the flowcell completely to the bottom. Make sure nothing is blocking the insertion!
4. Secure its position with the designated golden plate.

CYANSmart user manual, Version 20160902_(5.1) Page 15

 √ X
⑦ ⑥
⑥ ⑦ ⑥
⑦
Window

CYANSmart user manual, Version 20160902_(5.1) Page 16

2.3.5 Install the waste tube and waste bottle
1. The waste tube is delivered with the connector attached.
2. Screw this connector on the waste connector on the back panel.
3. Insert the other side of the waste tube in the waste bottle.
! Place the tube deep enough to avoid it slipping out during measurement, but avoid inserting it too
deep so it does not reach the waste liquid level. This latter could cause poor drainage and increases the
risk of tube contamination!

End of tube

Waste level

2.3.6 Switch on the analyzer

At start-up, the instrument will perform a system self-test. After a start-up system test has been performed
successfully, the Cypress Diagnostics entering screen will appears. Touching this screen anywhere, will let
the user enter the main menu of the CYANSmart user software.

2.3.7 Install or change the print paper

1. Switch ON the instrument.
2. Open printer cover of the instrument and remove any left-over of the old print paper roll.
3. Take a new paper roll and place it into the paper slot. Make sure to place the paper in the right direction
(check attention point) and that the end is straight.
4. Roll off the print-paper until the beginning of the paper touches the printing bar.
5. When the instrument is ON and the paper touches the printing bar, the instrument will feed the paper
automatically. If this is not sufficient, press the “Printer feed” button on the touch screen, while keeping
the paper in contact with the printing bar.
6. Feed the paper until you can put it through the opening in the printer cover and close the printer cover.

CYANSmart user manual, Version 20160902_(5.1) Page 17

 Printer
Feed

! ATTENTION POINT!
Make sure to place the paper in the right direction. Only the coated side can be used to print. If the paper
is installed in the wrong direction (see below) the paper will move through the printer, but nothing will be
printed. To verify the printable side of the paper, the operator can scratch the paper. Upon scratching the
paper, only one side will become slightly greyish or shiny (see picture below), that is the side that can be
used for printing and thus should contact the printing bar.

√ X

Warnings
 Do not try to print without printing paper installed, this will cause a system crash!
 If the printer bar is (accidentally) removed from the printer head. Make sure to turn OFF the
instrument before repositioning it! Otherwise the cogwheels can get damaged!

2.3.8 Verify the instrument

1. During start-up, the instrument will automatically perform a ‘Self-test’. Verify that this was ok (3.1).
2. Perform a washing and cleaning.
During packing and transport, dirt can get in the tubes and on the instrument. Before starting the
measurements, it is thus recommended to perform a washing of the tubes (5.2.1) and to clean the
outside of the instrument (5.2.2).
3. Verify the aspiration and hydraulic system by performing a pump calibration (3.8).
4. Verify the optical system by performing an AD auto zero (3.9).
NOTE:
During transport of the CYANSmart analyzer, it is possible that the height of the lamp is slightly shifted.
This can result in out of range values for the gain during the AD auto zero. If after verification of all other
causes (see 3.9: clean, rinsed, no air bubble, instrument ON for at least 30 min), the problem of the higher
gain persist, please contact the technician of your distributor to adjust the height of the lamp.

2.3.9 Adaptation of the system to the end customer

1. System setup
 Adjust the print setup and standby time to the customer’s needs.
 Adjust the date format, and the date and time.
 Insert the name of the hospital or laboratory where the instrument is installed.
CYANSmart user manual, Version 20160902_(5.1) Page 18
 Adjust the language if necessary.
2. Customize the methods
If you wish to obtain results in a different unit than the one already preprogrammed, please follow this
procedure:
 Turn on the instrument and enter the main menu.
 Go to the “Methods” folder and select “Program Modify”.
 Select the program you want to modify.
 On page 1, select the desired Program units from the list.
 On page 2, adjust the linearity limits (min and max) and the reference range (normal low and normal
high value).
 On page 3, adjust the concentration of the standard/calibrator and the control P and control N ranges
(min and max).
Do NOT change any other parameter! The other parameters are independent of the unit!
It is essential that you correctly recalculate the parameters that are expressed in units!

Examples:
A. Conversion mg/dl to mmol/L
The conversion factor needed can be found on the insert. This value is different for every method!

Glucose liquid: the method is originally programmed in mg/dl, the desired unit is mmol/l. The conversion
factor (mentioned in the insert): mg/dl x 0.0555 = mmol/l.
Parameter to change Original Calculation New
(x conversion factor)
Program unit mg/dl - mmol/L
Linearity low 1.18 1.18 x 0.0555 0.0655
Linearity High 600.0 600 x 0.0555 33.3
Normal low 60.0 60.0 x 0.0555 3.33
Normal High 110.0 110 x 0.0555 6.105
Conc of STD 100.0 100 x 0.0555 5.55
Of cal Lot dependent - see insert 10.8
e.g. lot 2478
Control N min Lot dependent - see insert 4.46
e.g. lot 3577
Control N max Lot dependent - see insert 6.04
e.g. lot 3577
Control P min Lot dependent - see insert 11.8
e.g. lot 5567
Control P max Lot dependent - see insert 16.0
e.g. lot 5567
NOTE:
On the inserts of the calibrators and controls, different values are already mentioned (for different units).
Insert the value corresponding to the desired unit. If your unit is not mentioned on the insert, you need to
recalculate the value yourself (original x conversion factor).

B. Conversion mg/dl to g/L

1) First you need to calculate/ find the conversion factor
1 mg = 0.001 g and 1 dL = 0.1 L  1 mg/dL = 0.001/0.1 g/L = 0.01 g/L
Conversion factor: mg/dL * 0.01 = g/l
2) Recalculate the unit dependent parameters by multiplying them with this conversion factor.

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 For example 50 mg/dL * 0.01 = 0.5 g/L
NOTE:
For enzymatic reactions like GOT or GPT, concentrations will always be given in « U/l ». Therefore it is not
advised to change the units.

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3 SOFTWARE DESCRIPTION
! WARNING! Please read the user manual before operating the CYANSmart and make sure the
installation is performed correctly.
BIOHAZARD: Wear personal protective equipment. Use universal precautions. See 7. Safety
information for recommended precautions when working with biohazard materials.

3.1 SYSTEM START – “SELF-TEST”

Switch on the instrument from the main power switch located on the rear side of the instrument. During
the system start-up, the system will execute some self-tests. It will verify the correct functioning of the
crucial components present in the analyzer.

3.2 MAIN MENU

If the instrument functions correctly, the Cypress Diagnostics entering screen automatically appears.
Clicking this screen anywhere, will let the user enter the main menu of the CYANSmart user software.

The different submenu’s have the following functions:

Perform test To measure samples for a certain method.
Methods To setup the parameters for each test method.
Archives Sample and quality control result database, to (re)view, print and delete; and
view QC statistical information.
Tube wash To rinse/wash the hydraulic system (tubes + flowcell) of the instrument.
Printer feed To move the print-paper forward.
Pump calibrate To calibrate the aspiration steps for the pump.
AD zero To zero the instrument, and verify the functioning of the optical system.
Filter test To perform an OD measurement or verify the filters separately.
System setup Temperature display, print setup, standby setup, date format, date & time,
system information, hospital setup, language setup, and screen brightness.
Reserved settings Contains the password protected settings: Temperature calibration, filter and
cuvette setup, aspiration format, heading setup.
CYANSmart user manual, Version 20160902_(5.1) Page 21
3.3 PERFORM TEST MENU
For the best results, we advise to use Cypress Diagnostics reagents. The CYANSmart is delivered with all
Cypress Diagnostics biochemistry test methods pre-programmed. These methods have been extensively
verified and optimized. In the application sheets you can find detailed information on the preparation of
the samples and execution of the tests for all Cypress Diagnostics kits. Using these pre-programmed
methods and following the preparation instruction correctly, Cypress Diagnostics guarantees the accuracy
and precision as stated in the kit’s insert. You can always include other methods of your choice.
NOTE:
For best use of our kits on a Cypress Diagnostics analyzer we kindly advise to follow the application sheets
of the respective analyzer (NOT the kit insert). Upon registering to our website (www.diagnostics.be) you
can find the latest application sheets under the analyzers.
After selecting the “Perform test” folder, a list with the programmed methods will appear. Scroll through
the different methods by using the next page and previous page buttons. Select the desired method by
tapping it.

NOTE:
If the programmed temperature is not reached yet, the following screen will appear when opening the
method. After start-up, the instrument needs 15 - 30 minutes to obtain the correct working temperature.
This time is also essential to stabilize the lamp. It is thus strongly recommended to NOT proceed if the
temperature has not yet reached 37°C. Proceeding can affect the measurement accuracy and precision!

Setting The required temperature for the cuvette/flowcell as programmed in the method
parameters.
Measured The temperature of the flowcell currently measured by the instrument.
Ignore To continue the test without taking the temperature into account.
Wait To wait until the required temperature is reached. When the measured
temperature is the same as the set temperature, the following screen will
automatically be shown.
Exit To exit the test and to return to the main menu.

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The following screens will guide the user through the test procedure. The software will inform the
operator what to perform: AD auto zero, blank, standard, control or sample measurement; and will indicate
the acceptable range to facilitate the decisions the user has to make.
The different menus will now be explained using the end point test glucose liquid. If differences exist for
the other method types, these are explained in 3.3.7 below.

Operating tips: how to aspirate the liquids into the flowcell.

Under the aspiration inlet there is a lever (the aspiration button). Upon pressing the aspiration button (for
no more than half a second), the peristaltic pump is activated and the correct amount of liquid volume
(according to the method settings 500 - 1000μl) is aspirated in the flowcell, where it will be measured.
In order to reduce interference, carryover and the risk of contamination, an air gap will be automatically
aspirated between the samples.

Instruction for a correct aspiration:

1. Prepare disposable test tubes with correct amount of reagent and
sample volume according to the method.
2. Position the aspiration tip inside the test tube, be sure that the
Test tube
aspiration tip is in the lower corner of the test tube (see figure).
3. Press the aspiration button, the sample will be aspired automatically.
4. After aspiration is completed (no more pump motor sound), remove Aspiration tip
the aspiration tip from the liquid, so air can aspirated (=air gap).

To ensure a correct measurement, the flowcell should be filled with liquid and free of air bubbles. This filling
can be easily checked by opening the optical cover. Check the tubes going to and from the flowcell.
- The aspiration tube has to be completely filled with liquid and free of air bubbles from the fastening
screw to the flowcell.
- The tube from the flowcell to the pump should be filled with liquid and free of air bubbles for at
least 4 cm from the flowcell
If these tubes are filled with liquid until the limits indicated below and are free of air bubbles, the flowcell is
correctly filled. Otherwise check 3.8.
! ATTENTION!
- In the normal aspiration mode, the tubes have to be checked before the aspiration of the air gap!
- Close the optical cover for further measurements. Background light can interfere with the
measurements!

Tube from flowcell to pump, air bubbles only visible after

the 4 cm limit.

Aspiration tube completely filled with liquid,

no air bubbles

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3.3.1 AD auto zero (AD Value)
The first step in the test procedure is always the zero/reference measurement (AD auto zero) for the filter
used in the method. This measurement is always performed with distilled water.
NOTE:
To have a correct reference measurement, it is essential that the hydraulic system (tubes and flowcell) is
clean. Therefore it is recommended to rinse the CYANSmart with distilled water by pressing the “Tube Wash”
button for 10 times (=15 mL) BEFORE you perform a test.

1. Press “Continue” to start the AD Auto zero measurement.

2. Position distilled water under the inlet pipe and press the aspiration button.
3. The distilled water will be aspirated in the flowcell and the instrument will perform the zero
measurement.
4. When the measurement is finished, the AD measurement will be displayed (and printed).
5. This AD value should be between 50000 (AD Low) and 60000 (AD High).
a. If the AD Value is within range, press “Continue” to proceed to the next screen.
b. If NOT (The error “L” or “H” will be displayed behind the value), press “Rerun” to repeat the
measurement. Do NOT continue measuring if the AD value is outside the acceptable range!
! ATTENTION! If after 2 reruns the value is still outside the acceptable range. Check:
 Rinsing with 15mL distilled water (see 5.2.1).
 Cleaning with detergent and/or 5% hypochlorite (see 5.2.1).
 Pump calibration and verify that the flowcell is completely filled with distilled water and free of air
bubbles (see 3.8).
 AD auto zero to verify the performance of the complete optical system (see 3.9).
3.3.2 Test Blank
The blank measurement is essential in the calculation of end point tests, but will, in addition, also provide
an indication of the reagent deterioration or the cleanness of the instrument. Check the application sheets
if reagent or distilled water needs to be measured and for the number of blank measurements that are
recommended to be performed.

1. The first screen will show the OD value obtained during the last blank measurement of that method.
2. Press “YES” to perform new blank measurement. It is strongly recommended to measure a new blank
every time you perform a test.
If “NO” is selected, the instrument will use (and print) the last blank value to calculate the result of the
measurements and proceed to the standard measurement.
3. Position the test tube containing the blank (reagent or distilled water) under the inlet pipe and press
the aspiration button.
4. The blank will be aspirated in the flowcell and the instrument will perform the measurement.
CYANSmart user manual, Version 20160902_(5.1) Page 24
5. When the measurement is finished, the OD measurement (= absorbance of the blank) will be displayed.
NOTE: For some methods, it is recommended to perform multiple blank measurements. Then the
request to aspirate will re-appear after aspiration of the blank until the programmed number of blanks
have been aspirated. Then all (up to 3) measurement results will be displayed (and printed).
6. This OD value should be between the range mentioned for “Blank Low” and “Blank High”.
a. If the OD Value is within range, press “Continue” to proceed to the next screen.
b. If NOT (The error “L” or “H” will be displayed behind the value), press “Rerun” to repeat the
measurement. Do NOT continue measuring if the OD value is outside the acceptable range!
! ATTENTION! If after 2 reruns the value is still outside the acceptable range:
- Water as blank
 Clean and then rinse instrument abundantly with distilled water and restart the test.
- Reagent as blank
 Check (working) reagent preparation.
 Reagent is deteriorated, check storage conditions (expiration date and opening date), open new kit
or vial and restart the test.

3.3.3 Test standard/calibrator

The standard or calibrator measurement is essential for the determination of the factor and thus in the
calculation of all sample tests results. Check the application sheets if a standard, calibrator or factor should
be used. If STD = 0 is programed, the software will not ask to test a STD (see 3.4) and immediately proceed
to the quality control (QC) measurements.

1. The first screen will show the factor obtained during the last calibration of that method.
2. Press “YES” to perform a new standard measurement. A new calibration should be performed:
a. First time you perform the test method.
b. When using a new lot number of reagent or new working solution.
c. When the QC measurement is out of range.
Press “NO” to keep (and print) this factor and immediately proceed to the QC measurements.
3. Position the test tube containing the standard preparation under the inlet pipe and press the aspiration
button.
4. The standard preparation will be aspirated in the flowcell and the instrument will perform the
measurement.
5. When the measurement is finished, the OD value measured will be displayed (and printed).
6. Taking into account the programmed concentration of the standard, the instrument will calculate,
display (and print) the new calibration factor.
a. Press “Continue” to proceed to the next screen.
b. Press “Rerun” to repeat the measurement.
NOTES:
1. Some standard and (almost) all calibrators have a lot dependent concentration! Make sure the correct
concentration is programmed in the methods! Check or modify in “Methods” “Program modify” 
Select method STD Conc (Units!) (See 3.4.2).
2. Some methods do not have a standard included in the kit. For these methods, a factor is pre-
programmed. You can use this factor if the quality control (QC) measurements are within range.
However, for the most accurate results, it is recommended to use a calibrator (see application sheets) to

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 calculate the factor. This requires adjusting the settings of the method; program NUM of STD: 1 and
CONC: as mentioned on the insert/vial.
3. If after recalibration with the standard included in the kit, the QC measurements remain out of range, it
is recommended to use a calibrator to perform a more accurate calibration (see application sheets).
4. In case of a multi-standard, aspirate the standards from the lowest to the highest concentration. This
should also be programmed this way in the “Methods” menu. The result screen will show the graph
obtained from these measurements instead of a single factor.

3.3.4 Test quality control N/P

Performing quality control measurements is the only way to ensure that the results you obtain are accurate.
If the QC measurements are within range, you can be sure the correct procedure is used (pipetting, storage,
contamination ...), that the analyzer is clean and correctly functioning, that the reagent is not deteriorated
and that the calibration is performed correctly. This will result in more accurate results, thereby less false
negative and positive results and thus exclusion of false diagnosis. This will also eliminate the need to
duplicate a test because you are not sure of the result. All advantages for the patient, laboratory and doctor.
It is strongly recommended to perform a Quality Control (QC) by measuring both normal control and
pathological control serum. Check the application sheets which control sera should be used.

1. Press “YES” to perform a new normal control measurement. Ideally, both controls are measured every
time you perform a test. QC measurements should at least be performed:
a. First time you perform the test method.
b. When using a new lot number of reagent or new working solution.
c. Once a week.
If “NO” is selected, the instrument will proceed to the control P / sample measurement.
2. Position the test tube containing the QC preparation under the inlet pipe and press the aspiration
button.
3. It will be aspirated in the flowcell and the instrument will perform the measurement.
4. When the measurement is finished, the OD measurement and calculated concentration will be
displayed (and printed).
5. Check if the concentration is within target control range.
a. If the concentration is within range, press “Continue” to proceed to the next screen.
b. If NOT (The error “L” or “H” will be displayed behind the value), press “Rerun” to repeat the
measurement. Do NOT continue measuring if the concentration is outside the acceptable
range!
6. Repeat these steps for the pathological control.

! ATTENTION! If after the rerun the value is still outside the acceptable range. Check:
 The preparation instructions (application sheets): stability, volumes, and times should be strictly
followed.
 Rinsing with 15 mL (10x) distilled water (see 5.2.1).
 Cleaning with detergent and/or hypochlorite (see 5.2.1).
 Pump calibration and verify that the flowcell is completely filled with distilled water and free of air
bubbles (see 3.8).
 AD auto zero to verify the performance of the complete optical system (see 3.9).
 Restart the measurement and include a new calibration!
CYANSmart user manual, Version 20160902_(5.1) Page 26
NOTES:
1. Quality controls have a lot dependent concentration! Make sure the correct range is programmed in
the methods! Check or modify in “Methods” “Program modify”  Select method  Control min/max
(Units!) (See 3.4.2).
2. If after recalibration with the standard included in the kit, the QC measurements remain out off range,
it is recommended to use a calibrator to perform a more accurate calibration (see application sheets).

3.3.5 Sample measurement

If the AD value, blank and QC are OK, you can proceed with the sample measurements.
The first screen will show the unique (unchangeable) sequence number of this sample measurement.

Nr Unique (unchangeable) sequence number of this sample measurement.

Ref Reference name/number for the sample. By clicking this box, an alphanumeric
keyboard will appear. The reference can contain up to 16 digits. Click “OK” to exit
the keyboard view.
NOTE:
If during the measurements, there is not sufficient time to insert the reference, this
can added after finishing the tests by editing the sample results in the archive menu
(see 3.5.1). In this case, make sure to write down the sequence number to keep track
of the samples!
Feed To move the print paper forward without printing.
Wash To rinse the hydraulic system (tubes + flowcell) before aspirating the sample.
 In case of high risk of cross-over, the flowcell can be pre-rinsed by aspirating
distilled water, followed by air (to avoid dilution) between the samples.
 In case of a strongly colored reagent, it might be necessary to pre-rinse the
flowcell with (working) reagent before aspirating the first measurement.
Otherwise the first measurement could be influenced by the distilled water
used to zero the instrument.
Continue To proceed to the next screen.
Perform Test To return to the main menu. The instrument will ask for a confirmation. Press “YES”
to proceed or “NO” to return to the sample measurement.

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1. Position the test tube containing the 1e sample preparation under the inlet pipe and press the aspiration
button.
2. It will be aspirated in the flowcell and the instrument will perform the measurement.
3. When the measurement is finished, the OD measurement and calculated concentration will be
displayed (and printed).
4. Press “Continue” to proceed with the next sample.
5. Proceed like this until all the samples are analyzed.

3.3.6 End of test

1. Press “Perform Test” in the upper left corner to exit the test method.
2. The instrument will ask for a confirmation. Press “YES” to proceed or “NO” to return to the test
performance.
3. Rinse with distilled water, by pressing the “Tube WASH” button (10x). In total 15 ml of water will be
aspirated.

NOTE:
When the memory is full, a warning message will be displayed “Memory full! Original data will be
overwritten!”. It is recommended to print all the needed results and to delete them to make space in the
memory.

3.3.7 Specific method instructions and formulas

In the application sheets you can find detailed information on the preparation of the samples and execution
of the tests for all Cypress Diagnostics kits.
3.3.7.1 End point methods
After a certain incubation time, this type of reaction reaches an absorbance that remains stable for a specific
period. Within this stable period (at time t1), the measurement of the sample is performed. Before addition
of a sample (at time t0), the reagent already has a certain absorbance. To correct for this absorbance, the
reagent is measured as blank and the difference in absorbance of the sample to the blank is calculated.
CYANSmart user manual, Version 20160902_(5.1) Page 28
Calculation formula:
Conc. sample = F x (Abs sample-Abs blank) with factor F = Conc. standard / (Abs standard - Abs blank)
Implications for performing the test
1. Incubation time is performed outside the instrument.
2. You can prepare several samples simultaneously. Mix and incubate for the specified time at the
specified temperature. After the incubation time, aspirate and measure all the samples within the
specified stable period after preparation.
3. For some end point tests, the incubation time needs to be exact. A special time scheme can be used to
prepare several samples ‘simultaneously’, as long as you respect the times mentioned!
E.g. Bilirubin direct and total, LDL cholesterol, HbA1c, Haemoglobin, Phosphorus…
4. Correct measurement of the (reagent) blank is very important!
NOTE:
For an end point test where the absorbance of the sample itself can affect the analytical result, it is necessary
to measure and correct for the sample blank. This correction can be done by choosing one of the following
methodologies according to the specific need:
 Sample/serum blank: absolute correction
 Bichromatic: partial correction
3.3.7.2 Serum blank/Sample blank
Calculation formula:
Conc. sample = F x (Abs sample-Abs sample/serum blank) with factor F = Conc. standard / (Abs standard - Abs standard blank)
Implications for performing the test
1. For every measurement, you need to prepare 2 test tubes: one for measuring the sample/serum blank
(background absorbance) and one for measuring the real sample absorbance. Both are measured on
the same wavelength.
2. The above is valid for samples, but also for the standard/calibrator and control preparations.
3. The Blank method programmed is Serum. ! Pay attention! This determines the calculation of the
results, NOT the blank aspiration in the perform test menu!
1. Aspirate distilled water to adjust the instrument to zero (AD value).
2. In the blank menu aspirate (working) reagent (see application sheets).
3. In the standard/sample menu:
a. First: “Aspirate Serum” = Standard/Sample Blank  OD1
b. Secondly: “Aspirate Standard/Sample” = complete Standard/Sample  OD2

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3.3.7.3 Bichromatic
This type of methodology is applicable to end point reactions that require a correction due to biological
liquid’s properties of the sample (turbidity, icteric and hemolysis). The correction on the matrix is not
absolute, but relative. The bichromatic calculation requires measurement at two wavelengths: the main
filter and sub filter.
Calculation formula:
Conc. sample = F x (Abs main nm sample-Abs sub nm sample) with factor F = Conc. standard / (Abs main nm standard - Abs sub nm standard)
Implications for performing the test
For every measurement (AD value, blank, standard, QC and samples), the instrument will perform
measurements with both the main and sub filter and display the results (OD1 = main and OD2 = sub).

3.3.7.4 Two points (fixed time) methods

After a certain incubation time, this type of reaction has a linear progress. For each measurement/aspiration,
the instrument will carry out two readings within this linear time period, respectively at the times t1 and t2.
The analytical result can be calculated using Δ Abs (delta) = difference in absorbance at the times t2 and t1.
Calculation formula:
Conc. sample = F x (Abs sample t2-Abs sample t1) with factor F = Conc. standard / (Abs standard t1- Abs standard t2)

Implications for performing the test

1. Incubation time is IN the instrument.
2. You need to prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart,
immediately after addition of the (working) reagent to the sample/standard/control.
3. It is thus essential that the CYANSmart is ready for the aspiration. This is when the CYANSmart displays:
“Press PUSH to aspirate sample/standard/control”.
4. Wait with the preparation of the next sample until the CYANSmart is finished with measuring the
previous one.
5. The above is valid for samples, but also for the standard/calibrator and QC preparations.
6. The correct temperature is very important since temperature influences the rate of a reaction.

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3.3.7.5 Kinetic methods
This methodology type is used to determine enzyme activity. For each measurement/aspiration, the system
carries out a number of readings. From all the reading points taken during the reading time, the slope of a
theoretical straight line is determined, using the criteria of minimum squares. The slope is used to calculate
the analytical result. Furthermore, for every measurement, the instrument will display the linear correlation
coefficient (COR). This could be used as a control parameter for kinetic reactions. The closer to one, the more
perfect the fit resembles a straight line. The COR should be ≥ 0.95.

Calculation formula:
Conc. sample = F x ΔAbssample /min or (ΔAbssample x 60) / Δt
With F = fixed factor or F = (Conc.calibrator x Δt) / (ΔAbscalibrator x 60)
ΔAbssample = the sum of the absorbance of each inter zone of the dynamic test time = (AS2- AS1) + (AS3-
AS2) + … + (ASn-1- ASn-2) + (ASn- ASn-1)
Δt =the dynamic test time = (t2- t1) + (t3- t2) + … + (tn-1- tn-2) + (tn- tn-1)
Implications for performing the test
1. Incubation time is IN the instrument.
2. You need to prepare, mix and measure one sample at a time. Aspirate the mixture in the CYANSmart,
immediately after addition of the (working) reagent to the sample/calibrator/control.
3. It is thus essential that the CYANSmart is ready for the aspiration. This is when the CYANSmart displays:
“Press PUSH to aspirate sample/standard/control”.
4. Wait with the preparation of the next sample until the CYANSmart is finished with measuring the
previous one.
5. The above is valid for samples, but also for the standard/calibrator and QC preparations.
6. The correct temperature is very important since temperature influences the rate of a reaction and the
activity (factor).
Press the aspiration button to aspirate the sample. The CYANSmart will display the measurement result and
reaction curve expressed as absorbency in real time during the testing. At the end of the measurement the
absorbance rate, correlation, and concentration will be displayed and the results will be printed.

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CYANSmart user manual, Version 20160902_(5.1) Page 32
3.4 METHODS
After selecting methods folder, you can add, modify or delete a program or print the method list.

3.4.1 Program Add

The instrument is delivered with all Cypress Diagnostics biochemistry methods already pre-programmed
and ready to use. These optimized parameters can always be reviewed in the application sheets (end of user
manual or website for the latest update). You can always include other methods of your choice. The
instrument can store 180 methods. Once the store capacity is full, a warning will be shown: ”Program Result
Full! Please Delete!”
Pressing the program add button, will open the first page of the method setup folder. Fill in all the fields. In
the areas that have an arrow, tapping the arrow will give a list of the possibilities that can be entered in
that area. In the following print screens, the parameters for glucose liquid are filled in as an example.

Program Name Use the keyboard that will appear if you tap this area, to insert the desired name.
Program Method Press the arrow and select the required test method in the pull-down menu: End
point, Two point, Kinetic or Bichromatic.
Main Filter Use the up or down arrow to select the required filter: 340, 405, 492, 510, 546, 578
or 620 nm.
Sub Filter This option is only available when the bichromatic method is selected. Otherwise
“None” is indicated. Use the up or down arrow to select the required reference filter:
340, 405, 492, 510, 546, 578 or 620 nm.
Program Unit Press the arrow and select the required program unit in the pull-down menu: g/dL,
g/L, mg/L, mg/dL, U/L, μmol/L, U/mL, mmol/L, μg/mL, ƞg/mL, μg/dL, ng/dL, ƞmol/L,
mEq/L, IU/L, mIU/L, μIU/mL, or μg%, %.

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 After the unit is programmed, the selected unit will automatically appear in all the
fields of the parameters with unit dependent values.
Aspiration volume Insert the required volume using the numeric keyboard that will appear. The limits
are 500 – 1000 μl.
Delay Time This is the incubation time inside the flowcell. Insert the required time using the
numeric keyboard that will appear. The limits are 0 -999 seconds.
NOTE: A minimum of 1 second is recommended to have a stabilization in the
flowcell after aspiration.
Test time This is the measuring time. Insert the required time using the numeric keyboard
that will appear. The limits are 3 -999 seconds.
NOTE: If the selected test time is less than 3 seconds, the sample is not measured!
Dilution Factor If manually a dilution of the samples is made, the dilution ratio can be inserted here.
This factor will then be taken into account in the calculation of the result. The
default is 1 (no dilution).
Press “Methods” any time to return to Methods folder or after finalization press “Next Page” to continue to
the second page.

Linearity Min The lower limit for linearity, expressed in concentration. If the patient result is below
this range, the result will be flagged (“*”).The measurement should be repeated
with new or more concentrated sample. The Insert the value using the numeric
keyboard that will appear.
Attention: Insert the value corresponding to the units of this method
programming!
Linearity Max The upper limit for linearity, expressed in concentration. If the patient result is
above this range, the result will be flagged (“*”). The measurement should be
repeated with diluted sample. Insert the value using the numeric keyboard that will
appear.
Attention: Insert the value corresponding to the units of this method
programming!
Blank Press the arrow and select the required blank method: water (bi-distilled), reagent
or serum (= sample blank). The serum or sample blank measurement can only be
performed for an end point method.
Num of Blank Insert the required number of replicates using the numeric keyboard that will
appear. The limits are 1-3.
Blank Low The lowest acceptable absorbance value for the blank measurement. Blank
measurements below this value will automatically be flagged (“L”). Insert the value
using the numeric keyboard that will appear.

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 If reagent is measured as blank, a value below this limit could give an indication of
reagent deterioration (especially for downward reactions).
Blank High The highest acceptable absorbance value for the blank measurement. Blank
measurements above this value will automatically be flagged (“H”). Insert the value
using the numeric keyboard that will appear.
If water is measured as blank, a value above this limit could give an indication of
contamination of the water or of the hydraulic system of the instrument.
If reagent is measured as blank, a value above this limit could give an indication of
reagent deterioration (especially for upward reactions).
Normal Low The minimum value of the normal reference range, expressed in concentration. If
the patient result is below this range, the result will be flagged (“L”). Insert the value
using the numeric keyboard that will appear.
Attention: Insert the value corresponding to the units of this method
programming! The values on the insert or pre-programmed in the instrument are
for orientation purpose. Each laboratory should establish its own reference range.
Normal High The maximum value of the normal reference range, expressed in concentration. If
the patient result is above this range, the result will be flagged (“H”). Insert the value
using the numeric keyboard that will appear.
Attention: Insert the value corresponding to the units of this method
programming! The values on the insert or pre-programmed in the instrument are
for orientation purpose. Each laboratory should establish its own reference range.
Press “Back” at any time to return to the previous page or after finalization press “Next Page” to continue to
the third page.

Insert the numbers of standards using the numeric keyboard that will appear. In
Num of STD case of a calibration by means of a factor, insert 0 and proceed with inserting the
factor. Insert a value ranging from 1 – 6, in case of a single or multi-point calibration.
STD The number displayed (from 0 - 6) is the “name” (reference) of the standard, for
which the concentration needs to be inserted. In case of a multi-point calibration,
use the arrow keys present in the STD area, to switch between the different STD’s
and insert their respective concentration.
CONC The concentration of the standard corresponding to the number displayed in STD.
Insert the factor using the numeric keyboard that will appear.
In case of a multi-calibration, the standard concentrations have to be entered from
the lowest to the highest value.
Attention: Insert the value corresponding the units of this method programming!
2 For some standards and in case a calibrator is used, the value is lot dependent,
thus needs to be adjusted for a new lot number.
CYANSmart user manual, Version 20160902_(5.1) Page 35
 Factor Value essential for the calculation of the concentration from the absorbance
measurement. In case of standard calibration, the factor will be automatically filled
in by the instrument after the calibration has been performed. In case of factor
calibration, insert the factor using the numeric keyboard that will appear.
Control N min The minimum value of the range of the normal control, expressed in concentration.
If the control measurement is below this range, the result will be flagged (“L”). Insert
the value using the numeric keyboard that will appear.
Attention: 3The value is lot dependent, thus needs to be adjusted for a new lot
number. Insert the value corresponding to the units of this method programming!
Control N max The maximum value of the range of the normal control, expressed in concentration.
If the control measurement is above this range, the result will be flagged (“H”).
Insert the value using the numeric keyboard that will appear.
Attention: 3The value is lot dependent, thus needs to be adjusted for a new lot
number. Insert the value corresponding to the units of this method programming!
Control P min The minimum value of the range of the pathological control, expressed in
concentration. If the control measurement is below this range, the result will be
flagged (“L”). Insert the value using the numeric keyboard that will appear.
Attention: 3The value is lot dependent, thus needs to be adjusted for a new lot
number. Insert the value corresponding to the units of this method programming!
Control P max The maximum value of the range of the pathological control, expressed in
concentration. If the control measurement is above this range, the result will be
flagged (“H”). Insert the value using the numeric keyboard that will appear.
Attention: 3The value is lot dependent, thus needs to be adjusted for a new lot
number. Insert the value corresponding to the units of this method programming!
Cuvette Temp Press the arrow and select the required flowcell temperature: room temperature
(R.T.), 25 °C, 30 °C or 37 °C. For all Cypress Diagnostics methods, 37 °C must be
selected.
Press “Back” at any time to return to the previous page or after finalization press “Done” to continue to the
final screen. Press “Yes” to save the test with the inserted parameters, the test will be added to the program
list. Press “No” to delete the test or “Cancel” to return to the previous screen.
If one of the parameters is not entered correctly or not entered at all; e.g. no program name, an error
screen will appear and will then automatically return to the method parameters.

3.4.2 Program Modify

This option can be used to check and/or to modify programmed test methods, change lot dependent
values, adjust program units…

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Scroll through the different methods by using the next page and previous page buttons. Select the desired
method by tapping it. You will see the same screens as for adding a method and the same parameters can
be viewed or adjusted.

3.4.3 Program Delete

Scroll through the different methods by using the next page and previous page buttons. Select the desired
method by tapping it. The software will ask “Sure to delete?” Select “Yes” or “No”.

3.4.4 Program List

Print List The CYANSmart will print out an overview of all programmed methods. No detailed
information of the methods is visible or printed.
Item print Scroll through the different methods by using the next page and previous page
buttons. Select the desired method by tapping it. The CYANSmart will print out the
different parameters of the selected method. Below you can find an example of an
“item print” printout.

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 CYANSmart Normal Low: 60.0000
CYAN Hospital Normal High: 110.0000
023 Linearity range:
Program name: GLUCL 1.1800 – 600.0000 mg/dL
Program Method: End Point Dilution Factor: 1.0000
Program Unit: mg/dL NUM of STD: 1
Main filter: 510 nm CONC of STD: 100.0000 mg/dL
Sub filter: None nm Factor: 0.0000
Delay time: 000 sec Target control P range:
Test time: 003 sec 0.0000 – 0.0000 mg/dL
Blank: Reagent Target control N range:
NUM of Blank: 1 0.0000 – 0.0000 mg/dL
Blank low: 0.0000 Cuvette Temp 37.0 deg
Blank high: 0.3200

3.5 ARCHIVES
After selecting the archives folder, the user can review the sample results, make a patient print report or
delete sample results. There is also a menu with all quality control results and it is also possible to back-up
the patient data through the USB port.

3.5.1 Result List

Pressing the result list button, will give you the list of all stored sample results. The instrument can store up
to 6000 test results. All results receive a unique sequence number and are stored chronologically. Once the
memory is full, the system will overwrite the oldest results. A warning will be shown. ”Memory full! Original
data will be overwritten”. Print the results you require and delete results to clear the memory. Click
“Archives” to return to the archive menu.

CYANSmart user manual, Version 20160902_(5.1) Page 38

You can scroll through the different results by using the “Previous page” and “Next page” buttons and select
a test result to see more details.
Pressing the “Search” button will allow to give in search criteria to restrict the number of results or find a
specific test result. Fill in the sequence number, reference (name/number) or a date and click the respective
“Search" button. A list of result that meet the criteria will be shown. The message “No Data” will be shown if
no result that meets the entered criteria can be found.

By clicking a test result in the result list or search list, the details of that test become visible.

Edit Clicking edit, you can adjust the reference of the sample. If for example you did
not have time to fill in this information during the perform test. Click back to
store the new reference name in the memory.
Print To print out the information for that test result.
Delete To delete the test result from the memory. Afterwards, the list will display N/A
for that sequence number.
Data synchronization For more information check 6.4.
Result List To return to the list view.

CYANSmart
CYAN Hospital
18-09-2014 10:28:28
Ref: Cypress
Nr: 620
Program Name: GLUCL
OD.: 0.7710
CONC: 154.200 mg/dL H

CYANSmart user manual, Version 20160902_(5.1) Page 39

 Normal Low: 60.00000
Normal High: 110.0000

3.5.2 Delete all

In this menu, it is possible to delete all the patient data stored in the memory. To avoid accidental deletion
of results, the CYANSmart will asks for a confirmation when this button is selected.

3.5.3 Data Synchronization

For more information, please check chapter 6.4.

3.5.4 Print report

This function enables to print a patient report, this includes all test results of a certain reference
(name/number) performed on a certain date.
Upon entering this menu, the reference and date need to be filled in. The system will search in the memory
and then print all the test results of that reference on that date. The message “No Data” will be shown if no
result meets the entered criteria.
CYANSmart
CYAN Hospital
26-09-2014 14:22:21
Ref: Cyan
Item Result Range
CHOLL 210.00 120.00~200.00
CRE 0.80 0.60~1.40
GLUCL 154.20 60.00~110.00
GOTL 35.00 0.00~38.00
TP 6.20 6.60~8.30

3.5.5 Control manage

In this menu you can review the statistics of the results from the quality control measurements. Only results
that are measured as controls will be stored in this menu.

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3.5.5.1 Control Statistics
Select the method and the type of control for which you want to review the statistics: Normal control
(“Control N”) or Pathological control (“Control P”). To review the individual results used as data input for the
statistics, select the menu “Control result”.

The statistics include: the program name, normal range for the control (as programmed in the method),
number of measurements performed, the average (AVG) of the measurements, the standard deviation (SD)
and the coefficient of variation (CV%).

Curve To view the curve. Print To print out the curve.

Print To print out the statistics Exit To return to the statistics.
(numbers).
Statistic List To return to the method
selection.
NOTES:
1. This control menu does not check the accuracy of the result. Check the accuracy manually by taking into
account the target range programmed.
2. Only the results of the controls, measured as control in the perform test menu will be included in this list.

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3.5.5.2 Control result
In this menu you can review the results of the quality control measurements for each test method. Only
results that are measured as controls will be stored in this menu.
Select the method and the type of control for which you want to review the results: Normal control (“Control
N”) or Pathological control (“Control P”). A list of all stored results for that type of control of the selected
method will be shown. You can scroll through the results with the “Previous page” and “Next page” buttons,
return to the method selection by pressing the “Result List button” or view the details of a measurement by
clicking on it.

Click “Print” to print out the control result details.

Press “Delete” to remove this result from the memory and thus the control statistics.
Click “Result List” to return to the list view of the stored results for that control and that method.
3.5.5.3 Delete control result
This menu is used to delete all control results for a certain method. After selecting this menu, an overview
of the programmed methods is shown. Select the method for which you want to delete the control results.
A confirmation screen will request to confirm the deletion. The results for the normal as well as the
pathological control will be deleted. It is suggested to perform this when opening a new lot of the kit or
using a new control lot.
3.5.5.4 Delete all results
This menu is used to delete all the control values for all the methods. A confirmation screen will request to
confirm the deletion.

3.6 TUBE WASH

This button is used to activate the peristaltic pump to rinse/wash/disinfect the hydraulic system
(tubes + flowcell). Insert the aspiration inlet in the liquid you want to aspirate and press the
“Tube wash” button. The instrument will aspirate 1.5 mL of liquid. By pressing the button 10
consecutive times (=15 mL), the whole hydraulic system is rinsed. This function is used to:
Tube  Aspirate distilled water to rinse the system.
Wash  Aspirate detergent or hypochlorite solution to wash/disinfect the system.
 Aspirate air to dry the system.
This function is not only present in the main menu. In the perform test menu, there is also a button
to enable rinsing with reagent, distilled water or air in between the samples.
Furthermore, pushing the aspiration button (when this is NOT requested by the software, thus in absence
of the notification “Press Push to aspirate…”) has the same function.

3.7 PRINTER FEED

This button is used to move the print paper forward without printing and to load a new roll of
print paper (2.3.7).

Printeruser manual, Version 20160902_(5.1)

CYANSmart Page 42

Feed
 This function is not only present in the main menu. In the perform test menu, there is also a
button to enable advancing the paper and review the (printed) results in between the
samples.

3.8 PUMP CALIBRATION

The peristaltic pump tube is placed on the pump rotor that is turned by the pump stepper motor. This menu
enables the user to calibrate the peristaltic pump, this is to determine how many motor steps are necessary
to aspirate a certain volume. To ensure precise and accurate sample aspiration, it is strongly recommended
to calibrate the pump once a week.

Before performing a pump calibration it is essential to check that:

a) There is no leakage or blockage in the hydraulic system (flowcell + tubes).
b) The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water (5.2)
c) The pump tube is installed and connected correctly (2.3.3)
1. Select the “Pump Calibrate” menu. The number of steps registered during the last pump calibration is
shown.

2. Pressing the volume box, a numeric keyboard will appear, enter 3000 μl as the volume to aspirate and
press OK to exit the keyboard view.
3. Pipette exactly 3000 μl of distilled water into a test tube.
4. Position the aspiration tip inside test tube, be sure that aspiration tip is in the lower corner of the test
tube (see figure), so all liquid can be aspirated.
5. Press the aspiration button to start the aspiration.
6. Immediately and exactly when the tube is empty, press the PUSH key again to stop the aspiration.
7. The number of steps performed by the motor is displayed. The value should be between 18.000 and
28.000.
It is strongly recommended to perform this procedure at least 3 times. All obtained values should be
within this range and the difference between the obtained values should be smaller than 1000 steps.
8. By pressing the “Main Menu” button the software will exit this submenu. The software will ask “Sure to
save?” Click “Yes” to save and use this calibration as reference to intake any quantity of sample. Click
“No” to discard this calibration.
If the obtained values are outside the range or the difference between the replicates is more than 1000
steps:
a) Verify the pipette used and its precision/accuracy  calibration
NOTE: For a higher precision we advise to use CYANPipettes. Ask your distributor for more information
about the CYAN product range.

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b) Verify that after aspiration of distilled water, there is no big drop formation
at the tip of the aspiration tube. Drop formation indicates the presence of
leakage or blockage in the hydraulic system (tubes or flowcell):
 Leak in one of the tubes or the flowcell:
 Replacement is necessary, contact your service engineer.
 Blockage in one of the tubes can be caused by:
 Squashing of the tube, for example by the case, replace the tube if
damaged, contact your service engineer.
 Sticking together of the pump tube. To prevent this, it is
recommended to unhook the pump tube if the instrument is not used for more than one week.
 Accumulation of dirt in the tubes  perform a cleaning with hypochlorite (5.2.1), replace if
cleaning is not sufficient, contact your service engineer.
 Blockage in the flowcell:
 Accumulation of dirt in the flowcell  perform a cleaning with hypochlorite 5.2.1), replace if
cleaning is not sufficient, contact your service engineer.
c) Verify there is no lubricant on the pump tube or the rotor rollers. Too much lubricant can make the
rollers slip and increase the number of step  clean and remove the lubricant.
d) Verify that the rotor is turning smoothly, contact your service engineer otherwise.
e) If after these verifications, the values remain outside the ranges, please contact a service engineer. Do
NOT continue measuring with the instrument if the AD zero is out of range!

3.9 AD ZERO
The AD zero menu permits to verify the performance of the whole optical system (lamp, filters, flowcell and
pre-amplifier board) and automatically adjust the measuring for aging of the lamp.
Furthermore the values of gain and offset are used in the calculation of the test results. Consequently, to
ensure precise and accurate measurements, it is strongly recommended to perform an AD zero:
 Every day/morning.
 If the AD auto zero is outside the range in the perform test menu.
 At instrument installation.
 After installation of a new lamp.
Before performing an AD Zero it is essential to check that:
a) The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
b) The flowcell is filled with distilled water and without any air bubbles.
c) The instrument is ON for 30 min, because the lamp needs this time to stabilize.
d) The optical cover is closed.

1. Select the “AD Zero” menu. The result of the last performed AD zero is shown. It is recommended to
make a print-out (by pressing the “Print” button) to be able to compare with the new reading.
2. Position a tube with distilled water under the aspiration inlet tube and press the aspiration button to
aspirate the distilled water.

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3. Pressing the “Read” button, the instrument will perform a new reading and display the results.

Filter The filter wavelength expressed in nm. Every filter will be tested separately.
Gain The signal-amplification coefficient. This gain is automatically adjusted by the
instrument to obtain AD values between 50.000 and 60.000 up to a maximal
gain of 29 is reached.
AD The amplified and converted (from analog to digital) signal.
Offset To compensate for negative counts, the light beam is sent through a black filter
to determine the offset-value.
State Indicates “OK” if the values for that filter are within the acceptable ranges, or
“Error if one of the values is outside the acceptable range.
Optimal ranges Gain: between 8 and 16
AD: between 50.000 and 60.000
Offset: between -150 and +150
Read To start a new reading.
Print To print out the displayed results.
Main Menu To return to the main menu. This measurement that is last performed will be
saved and the gain and offset from this measurement will be used in the
calculations when performing tests.
Since the AD Zero should be performed every day, the variation between the new measurement and the
previous (displayed when opening the menu) should be small. If you compare the results (compare always
the same filter!),
 The difference in gain should be ≤ 2
 The difference in offset should be ≤ ±50).
If the difference is bigger or the obtained values are outside the range, check if:
- The instrument is ON for at least 30 min, because the lamp needs this time to stabilize.
- The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
 Perform washing and rinsing (5.2).
- The windows of the flowcell are clean (no dirt/fingerprints on the outside).
 Clean with wipes for glasses.
- The flowcell is filled with distilled water and without any air bubbles.
 Verify the pump calibration and the absent of leakages.
- If the gain of all/most filters is 15, 16 are more.
 The lamp should be replaced: contact your service engineer.
If after these verifications, the values remain outside the ranges, please contact a service engineer. Do NOT
continue measuring with the instrument if the AD zero is out of range!

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3.10 FILTER TEST
This menu can be used to perform an OD test or absorbance measurement. It is also used by the service
engineer during lamp replacement and optimization and for checking the individual filters and the optical
sensor.
Before performing a filter test it is essential to check that:
a) The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
b) The flowcell is filled with distilled water and without any air bubbles.
c) The instrument is ON for at least 30 min, this time is necessary for the lamp to stabilize and for
the flowcell and incubator to obtain the correct temperature.
d) The AD Zero is performed and within the acceptable ranges.

1. Press the “Filter Test” button to open the menu.

2. Select the required filter (340, 405, 492, 510, 546, 578 or 620 nm) using the blue arrows.
3. The gain (from the last performed AD Zero) will automatically be displayed.
Do NOT change this gain!
4. Position a tube with distilled water under the aspiration tube and press the aspiration button to
aspirate.
5. Wait for the water blank (AD0) value to ± stabilize.
To improve the speed of the stabilization, preheat the distilled water using the incubator.
6. When the AD0 value is ± stable, press the “AD Test” button. The value should be between 50.000 and
60.000. Otherwise, rinse with distilled water and repeat the measurement.
7. Press the “OD Test” button to continue to the sample measurement.
8. In the new screen, the water blank value from the moment you pressed the “AD Test” button, will be
displayed.
9. Position a tube with the 1e sample under the aspiration tube and press the aspiration button to
aspirate.
10. The instrument will measure the sample AD value and calculate the sample OD.

Print To print the result.

Next sample To continue with the next measurement. Position a tube with the 2e sample
under the aspiration tube and press the aspiration button to aspirate.
Return To exit the OD Test menu and return to the Filter Test screen.
Main menu To return to the main menu.

3.11 SYSTEM SETUP

The menu « System Setup » is used to adjust and display various function and parameters of the instrument:
temperature display, print setup, standby setup, date format, date & time, system information, hospital
setup, language setup, and screen brightness. Upon exiting this menu, by pressing the “Main Menu” button,
CYANSmart user manual, Version 20160902_(5.1) Page 46
the instrument will ask if you want to save the changes made. Click “Yes” to save the changed settings, “No”
to exit the menu without saving or “Cancel” to return to the System Setup menu.

NOTE:
In case of an electric shock, the parameters saved on the mainboard can be modified or deleted. Please ask
the technician of your distributor to check and if necessary readjust the settings.

3.11.1 Temp Display

This menu enables to verify if the temperature of the flowcell (cuvette) and incubator measured by the
instrument corresponds to the set temperature. The temperature is expressed in degrees Celsius. Press the
“System Setting” button to exit the menu.
a) For the flowcell/cuvette, it is possible to set different temperatures. Press the blue arrow to switch
between room temperature (R.T.), 25°C, 30°C, and 37°C. Allow 15 - 30 minutes for the instrument to
obtain the requested temperature. The measured temperature should be the set temperature ± 1°C.
b) The incubator has a fixed set temperature of 37°C. The measured temperature should be 37.0±1°C.
If the measured temperatures to not correspond to these specifications, contact a service engineer.

NOTES:
1. After start-up of the instrument, the instrument requires 15 - 30 minutes to reach the programmed
temperature.
2. If upon entering the perform test menu, the programmed temperature for the flowcell/cuvette is not
yet reached, the instrument will display a warning to inform the user of this discrepancy.

3.11.2 Printer Setup

This menu enables the user to switch on or off the automatic printing and to determine the print output.
These print options only affect the printing of results in the “Perform Test” menu. Press the “System Setting”
button to exit the menu.

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3.11.2.1 Result print
Use the blue arrow to view the available options: enable or disable. When this option is enabled, the
instrument will print all results automatically upon finishing a measurement. All “on demand” printing in
the menus “Methods”, “Archive”, “AD Zero” or “Filter Test” are not influenced by the choice in this option.
3.11.2.2 Curve print
Use the blue arrow to view the available options: enable or disable. When this option is enabled, the curves
obtained during the performance of a kinetic test will be printed.
NOTE:
Curves are only printed when the print mode “Normal” is selected. When the print mode is “Concise”, the
curves are never printed.

3.11.2.3 Print mode

Use the blue arrow to view the available options: normal or express. The express printing mode is a more
economic printing and uses less paper than the normal mode. See the examples below.

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Express mode

CYANSmart Control N
CYAN Hospital Target Control N Range:
30-09-2014 08:32:11 80.3000 – 109.0000 mg/dL
Program name: GLUCL OD.: 0.4881
Program Method: End Point Control: 92.5848 mg/dL
Main filter: 510 nm
Range: Ref.: Cyan1
60.0000 – 110.0000 mg/dL Nr.: 625
Linearity Range: OD.: 0.3658
1.1800 – 600.0000 mg/dL 68.3694 mg/dL
AD: 55504
Blank: 0.0205 Ref.: Cyan2
CONC: 100.0000 mg/dL Nr.: 626
OD.: 0.5250 OD.: 1.0564
Factor: 198.0000 205.1082 mg/dL H

Normal mode

CYANSmart CYANSmart
CYAN Hospital CYAN Hospital
30-09-2014 08:32:11 30-09-2014 08:33:51
Program name: GLUCL Program name: GLUCL
Program Method: End Point Control N
Main filter: 510 nm Target Control N Range:
Range: 80.3000 – 109.0000 mg/dL
60.0000 – 110.0000 mg/dL OD.: 0.4881
Control: 92.5848 mg/dL
Linearity Range:
1.1800 – 600.0000 mg/dL
CYANSmart
AD Auto Zero
CYAN Hospital
AD: 55504
30-09-2014 08:34:43
AD Low: 50000
Ref.: Cyan1
AD High: 60000
Nr.: 625
CYANSmart Program Name: GLUCL
CYAN Hospital OD.: 0.3658
30-09-2014 08:32:43 CONC: 68.3694 mg/dL
Blank Normal Low: 60.0000
Program name: GLUCL Normal High: 110.0000
Blank: 0.0205
Blank Low: 0.0000 CYANSmart
Blank High: 0.3200 CYAN Hospital
30-09-2014 08:35:23
CYANSmart Ref.: Cyan2
CYAN Hospital Nr.: 626
30-09-2014 08:33:11 Program Name: GLUCL
STD OD.: 1.0564
Program Name: GLUCL CONC.: 205.1082 mg/dL H
CONC.: 100.0000 mg/dL Normal Low: 60.0000
OD.: 0.5250 Normal High: 110.0000
Factor: 198.0000

3.11.3 Standby Setup

In this menu you can select the preferred time (in min) after which the instrument will go into a standby
mode. Press the “System Setting” button to exit the menu.
In this standby mode, less voltage is supplied to the lamp, thereby elongating its life time. The backlight of
the screen will also go out. Touch the screen anywhere to exit the standby mode.

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3.11.4 Date Format
This menu enables you to select the desired date format: YY-MM-DD, DD-MM-YY or MM-DD-YY. Use the
blue arrow in the blue bar to open the different options. Clicking the preferred one. Press the “System
Setting” button to exit the menu.

3.11.5 Date and Time

This menu enables you to input the correct time and date according to the format selected under the menu
Date Format. Setting the correct date & time is essential if you wish to re-find results in the archives because
the data is stored chronologically. Press the “System Setting” button to exit the menu.

3.11.6 System Info

The software version and hardware version of the CYANSmart can be found here. This is useful information
for the service engineer or support technicians and should be included in the repair report. Press the
“System Setting” button to exit the menu.

3.11.7 Hospital Setup

This menu enables the user to add a personalized heading (name of the hospital or laboratory) on the print
reports. Press the “System Setting” button to exit the menu.

3.11.8 Language Setup

The current version of the CYANSmart software is available in English, French and Spanish. Press the “System
Setting” button to exit the menu.

3.11.9 Screen brightness

The brightness of the touch screen is adjustable. In this menu the user can adjust the screen brightness by
means of a range going from 0 – 64, with zero the darkest state and 64 the brightest state. With aging of the
touch screen, it can necessary to increase the brightness. Press the “System Setting” button to exit the menu.

3.12 RESERVED SETTING

This folder is password protected. It contains parameters only intended to be reviewed or changed in the
factory or by a trained service engineer.

3.12.1 Temp calibration

This menu is used by the service engineer to re-calibrate the temperature settings of the flowcell and
incubator.

3.12.2 Filter and cuvette

This is a factory setting. Do not change these settings.

3.12.3 Aspirate format

Use the blue arrow to view the available options: “normal” or “express”. In the normal aspiration format, the
programmed volume of sample is aspirated in the flowcell and measured. When the measurement is
finished, an air gap of 500 μl is aspirated to prevent cross-contamination with/of the next sample. Then the
instrument will indicate it is ready to aspirate the next sample. In the express mode, the programmed
volume of sample is aspirated and this is almost immediately followed by aspiration of an air gap, and then
the measurement is performed. After measurement, the instrument is ready for aspiration of the next
sample. The volume of the air gap in the express mode can be set (20 μl – 800 μl). The recommended range
is 50-150 μl.
! Attention !: if the air gap is too big, air bubbles will flow into the flowcell during measurement, given false
results and if the air gap is too small, there is increased risk of cross-contamination.
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3.12.4 Heading setup
This is a factory setting. It contains the name of the instrument. Do not change this setting.

4 DAILY ROUTINE
NOTE:
For best use of our kits on a Cypress Diagnostics analyzer we kindly advise to follow the application sheets
of the respective analyzer (NOT the kit insert). Upon registering to our website (www.diagnostics.be) you
can find the latest application sheets under the analyzers.

4.1 PREPARATIVE WORK

4.1.1 Preparation of the analyzer
1. Switch ON the CYANSmart. After a short system initialization, the Cypress Diagnostics logo is shown.
2. Tap the logo to enter the main menu.
3. Wait around 30 minutes to allow the instrument to reach the optimal working temperature and for
the lamp to stabilize before proceeding to the test measurements. In the meantime, you can perform
the preparative work.
4. Check the liquid level of the waste container (empty if necessary).
5. Pre-rinse the CYANSmart with 15 mL of distilled water by pressing the “Tube Wash” button for 10 times.

4.1.2 Perform an AD zero

! ATTENTION! When performing AD Auto Zero, make sure that:
 The flowcell is washed and sufficiently rinsed with distilled water.
 The flowcell is completely filled with distilled water and free of air bubbles (~pump calibration).
 The obtained values are within the ranges.

4.1.3 Preparation of the reagent

For certain methods it is necessary to prepare a working solution. Check the application sheets at the end
of the user manual for specific requirements for each reagent.

4.1.4 Preparation of the samples

1. Prepare the blank as described in the application sheets.
2. Prepare the standard/calibrator and sample(s) as described in the application sheets.
3. Prepare the quality controls in the same way as the samples:
a. Take the same amount of control serum as indicated for the sample.
b. Respect the incubation times and use the same measuring procedure.

4.2 START SAMPLE ANALYSIS

Go to the “Perform Test” menu and select the test of your choice from the program list. Follow the work
flow as displayed on the screen and in the daily routine schematics:
If the programmed temperature is not yet reached, “Temp doesn’t match!” will appear when opening the
method. Click ‘Wait’. Do NOT proceed if the temperature has not reached 37 °C!

4.2.1 AD Value
1. Press “Continue” to start the AD Auto zero measurement.
2. Position distilled water under the inlet pipe and press the aspiration button.
3. The AD value should be within range AD Low (50000) and AD High (60000).
a. If the AD Value is within range, press “Continue” to proceed to the next screen.
b. If NOT (The error “L” or “H” will be displayed behind the value), press “Rerun” to repeat the
measurement. Do NOT continue measuring if the AD value is outside the acceptable range!

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 ! ATTENTION! If the values remain out of range, wash and clean abundantly with distilled water. If
after several washing and cleaning procedure the values remain out of range, perform an AD auto
zero.

4.2.2 Blank measurement

1. Check the application sheets if distilled water or reagent needs to be measured.
2. Select “YES”. It is strongly recommended to measure a new blank every time you perform a test.
3. Position the test tube containing the blank under the inlet pipe and press the aspiration button.
4. The OD value should be between the range mentioned for Blank Low and Blank High.
a. If the OD Value is within range, press “Continue” to proceed to the next screen.
b. If NOT (The error “L” or “H” will be displayed behind the value), press “Rerun” to repeat the
measurement. Do NOT continue measuring if the OD value is outside the acceptable range!
! ATTENTION! If after the rerun the value is still outside the acceptable range and blank is:
a. Water  Rinse instrument abundantly with distilled water and restart the test.
b. Reagent 1) Check the preparation of the working solution and the reagent.
2) Reagent is deteriorated, check storage conditions, open new kit and restart test.

4.2.3 Standard/calibrator measurement

1. Check the application sheets if a standard, calibrator or factor should be used. If STD = 0 is programed,
the software will not ask to test a STD.
2. Select “YES”
a. First time you perform the test method.
b. When using a new lot number of reagent or new working solution.
c. When QC measurement is out of range (see following point).
3. Position the test tube with the standard preparation under the inlet pipe and press the aspiration
button.
4. The factor will be automatically calculated and stored in the method programming.
NOTES:
1. Make sure the correct concentration is programmed in the methods (lot dependent concentration!)
Check or modify in “Methods” “Program modify”  Select method STD Conc (Units!).
2. Some methods do not have a standard included in the kit. For these methods, a factor is pre-
programmed. You can use this factor if the QC measurements are within range. However, for the most
accurate results, it is recommended to use a calibrator (see application sheets) to calculate the factor.
This requires adjusting the settings of the method; program NUM of STD: 1 and CONC: as mentioned on
the insert or the vial.

4.2.4 Quality control measurement

1. Check the application sheets which control sera should be used. For optimal guarantee of results, always
measure both normal control and pathological control serum.
2. Select “YES”
a. First time you perform the test method.
b. When using a new lot number of reagent or new working solution.
c. Recommended every time you perform that method or at least once a week
3. Position the test tube with the QC preparation under the inlet pipe and press the aspiration button.
4. Check if the concentration is within target control range.
a. If the concentration is within range, press “Continue” to proceed to the next screen.
b. If NOT (The error “L” or “H” will be displayed behind the value), press “Rerun” to repeat the
measurement. Do NOT continue measuring if the concentration is outside the acceptable
range!
NOTES:

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1. Quality controls have a lot dependent concentration! Make sure the correct range is programmed in
the methods! Check or modify in “Methods” “Program modify”  Select method  Control (Units!).
2. If even after recalibration with the STD included in the kit, the QC measurements are still outside the
range, it is recommended to use a calibrator to perform a more accurate calibration.

4.2.5 Sample measurement

1. If the AD value, blank and QC are OK, you can proceed with the sample measurements.
2. Insert the reference name/number of the sample and press “Continue”.
3. Position the test tube with the 1e sample preparation under the inlet pipe and press the aspiration
button.
4. Press “Continue” to proceed with the next sample and proceed until all the samples are analyzed.

4.3 END OF TEST

1. Press “Preform Test” to exit the test method and confirm.
2. Rinse with 15mL distilled water, by pressing the “Tube Wash” button at least 10 times.

4.4 EVALUATE THE RESULTS

1. The results (OD and concentration value) can be printed with the built-in thermal printer.
2. In the archive menu, review the sample results can be reviewed, edited (addition of reference), printed
or deleted and a patient print report can be made.
3. The control results are stored in a separate control menu and statistics can be viewed.

4.5 SHUT DOWN - END OF THE DAY

1. Wash the instrument with a detergent (CYAN Washing Solution CY001-WS or 5% Tween 20).
a. Position the detergent solution under the inlet pipe.
b. Press the “Tube Wash” button at least 10 times to aspirate 15mL of detergent.
c. Repeat this operation using air at least once to make an air gap between the detergent and the
distilled water.
d. Repeat this operation using 15mL distilled water.
e. Repeat this operation using air at least 10 times (=15 mL) to dry the hydraulic system.
2. Switch OFF the CYANSmart.
3. Clean the external surface of the instrument, with a cloth and some detergent.

4.6 WEEKLY
1. Perform a pump calibration and make sure to:
 Pipet exactly 3000 μl of distilled water.
 Press the aspiration button immediately after all the liquid has been aspirated.
 Perform in triplicate (3 repetitions).
 Obtain values between 18.000 and 28.000 and the difference between repetitions <1000.
2. Wash the instrument with a detergent (see end of day).
3. Disinfect the hydraulic system (tubes and flowcell) with 5% Hypochlorite (bleach).
a. Position the hypochlorite solution under the inlet pipe
b. Press the “Tube Wash” button at least 10 times to aspirate 15mL of hypochlorite solution.
c. Incubate the hypochlorite in the flowcell for about 10 minutes (no longer!).
d. Aspirate air (1x) and then rinse the instrument with 15mL distilled water and air (10x).

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 DAILY ROUTINE SCHEMATICS
PREPARATION OF THE ANALYZER
1. WAIT 30 MIN (lamp + temp)
2. CHECK WASTE LEVEL
3. PRERINSE WITH DISTILLED WATER
4. PERFORM AD AUTO ZERO

PREPARE (WORKING) REAGENT

PREPARE BLANK / STD/ QC / SAMPLES

PERFORM TEST –SELECT METHOD

AD VALUE

1e CLEANING NO
e
OK?
2 AD AUTO ZERO
3e PUMP CALIBRATION YES NEW KIT & CHECK
BLANK MEASUREMENT STORAGE CONDITION

NO REAGENT DETERIORATION

OK?
YES
INCLUDE
(STD/CAL MEASUREMENT)

CONTROL MEASUREMENTS

NO
CHECK PREPARATION

OK? YES
SAMPLE MEASUREMENTS

END OF DAY END OF WEEK

CLEAN WITH DETERGENT

CLEAN WITH DETERGENT
CLEAN WITH 5% HYPOCHLORITE
RINSE WITH DISTILLED WATER
RINSE WITH DISTILLED WATER
RINSE
CYANSmart user manual, WITH
Version AIR
20160902_(5.1) Page 54
RINSE WITH AIR
5 MAINTENANCE
This chapter contains all routine operations concerning instrument maintenance. The procedures, listed
and described below, should be carefully followed in order to guarantee the manufacturers quality
specifications and the perfect working conditions of the instrument over time.
The CYANSmart requires three levels of maintenance:
 Punctual maintenance: at special moments such as at installation.
 Routine maintenance: to maintain the level of precision day in day out.
 Special maintenance: replacement of specific parts.
Please consult the program maintenance form for an overview of the different maintenance actions. In case
of repair, please register all undertaken actions in the repair form in order to keep track of the interventions.

5.1 PUNCTUAL M AINTENANCE

The punctual maintenance should be effectuated at installation, before or after a long period of duty.
The following points of attention should be considered:
 Check the installation instructions.
 Check all tubes visually for leaking or squashing. Pay special attention to the pump tube. Replace if
necessary.
 Removing dust from the instrument.
 Washing the flowcell with a detergent (CYAN Wash Solution CY001-WS or 5% Tween 20) and rinse
the flowcell with distilled water.
 Disinfect the flowcell with 5% Hypochlorite solution and rinse the flowcell with distilled water.
 Check the pump calibration.
 Check the AD auto zero and replace the lamp/filters if necessary
 In case the instrument will not be used for a long period:
o Remove all liquid inside the instrument by aspirating air.
o Disconnect the pump tube.

5.2 ROUTINE MAINTENANCE

Frequency What Cleaning solution
Between methods Rinsing Distilled water
End of the day Detergent + rinse with distilled water + rinse with air
Washing Detergent + sodium hypochlorite (diluted 5 %) + rinse
Every week
with distilled water + rinse with air
End of the day (outside) CYDIS surface/detergent + dust cover
Cleaning
Every 6 months (inside) Pressurized air (by service engineer)!
Every morning AD auto zero
Verification
Every week Pump calibration

5.2.1 Washing
1. Between tests
It is not necessary to rinse the instrument between different samples for the same method. However:
 In case of high risk of cross-over, you could pre-rinse the flowcell by aspirating distilled water, followed
by air (to avoid dilution) between the samples.
 In case of a strongly colored reagent, it might be necessary to pre-rinse the flowcell with (working)
reagent before aspirating the first measurement. Otherwise the first measurement could be influenced
by the distilled water used to zero the instrument.

Between methods, always rinse the flowcell with distilled water, to avoid contamination problems.

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2. Daily washing
At the end of each working day, wash the instrument with a neutral detergent (CYAN Washing Solution
CY001-WS or 5% Tween 20):
a. Position the detergent solution under the inlet pipe.
b. Press the “Tube Wash” button at least 10 times to aspirate 15mL of distilled water.
c. Repeat this operation using air at least once to make an air gap between the detergent and the
distilled water.
d. Repeat this operation using 15mL of distilled water to rinse the system.
e. Repeat this operation using air at least 10 times (=15 mL) to dry the hydraulic system and avoid
microbiological contamination.

3. Weekly washing
After a week of work, wash first with 15 mL detergent (end of day procedure); and then disinfect the
hydraulic system (tubes and flowcell) with 5% Hypochlorite (bleach).
a. Position the hypochlorite solution under the inlet pipe
b. Press the “Tube Wash” button at least 10 times to aspirate 15mL of hypochlorite solution.
c. Incubate the hypochlorite in the flow cell for about 10 minutes (no longer!).
d. Aspirate air (1x) and then rinse the instrument with 15mL distilled water and air (10x).
NOTES:
1. In case of air bubbles (visible during AD auto zero or perform test) rinse with diluted sodium
hypochlorite (5 to 10%), then rinse with plenty of distilled water, followed by a few washing cycles with
air.
2. This diluted sodium hypochlorite is normally market available. Please use only good trademarks. Cheap
ones contain contaminating solutions.
3. Always dilute the sodium hypochlorite for 5 to 10%, never use it undiluted! This will damage the
instrument.
4. Do not use corrosive detergents to wash the instrument.

5.2.2 Cleaning
1. Daily cleaning
Please notice that the instrument, especially the optical system and the electrical circuit, are sensitive to
dust. Avoid using the instrument in dusty spaces. Cover the instrument when not in use and keep the printer
and flowcell cover closed during use.
At the end of each working session, dampen a tissue with a non-abrasive detergent like CYDIS Surface (Cod.
CYDIS-S) and wipe the outside of the instrument.

2. Every 6 months
It is recommended to clean the dust from inside the instrument at least every 6 months. To do so, remove
the cover and blow the dust off the instrument using pressurized air. Make sure the air is completely dry!
Open the filter cover and blow the dust off.
NOTES:
1. The instrument cover should only be removed when the CYANSmart is unplugged.
2. We strongly advise to leave this procedure to the technical assistance of the service engineer of your
distributor.

5.2.3 Verification
1. Daily
Perform an AD auto zero.

2. Weekly
Perform a pump calibration.

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5.3 REPLACEMENT OF SPECIFIC PARTS
Before replacing any instrument part, repairing any defective items or performing any instrument
maintenance procedure(s), the operator or maintenance technician must carry out the below-described
decontamination procedure of the instrument part(s) involved in the operation(s).

5.3.1 External cleaning

Use CYDIS Surface (Cod. CYDIS-S) or a 6% ESOFENOL solution (60 cc in one liter of distilled water). These are
antibacterial and antiviral substances.
 Spray the solution all over the instrument.
 Allow the solution to stand for approximately 30 minutes.
 Wipe the solution off the instrument using a sponge dampened with distilled water.

5.3.2 Internal washing

Wash first with 15 mL detergent (end of day procedure); and then disinfect the hydraulic system (tubes and
flowcell) with 5% Hypochlorite (bleach). Make sure to incubate for 10 minutes and then rinse sufficiently
with distilled water. (5.2.1).

5.3.3 Replacement schedule

Some spare parts should be replaced after a fixed period of time to ensure the good performance of the
analyzer. Please check the table below:
Spare part Replacement Who?
Peristaltic pump tube Every six months User / service engineer
All tubes of hydraulic Every year or in case of leaking/obstruction or Service engineer
circuit strong coloration
Lamp Every year Service engineer
Amplifier board Every 2/3 years (together with lamp replacement) Service engineer
Peristaltic pump Every 2/3 years (together with pump tube Service engineer
rubbers replacement)
Connectors Every 2/3 years (together with tube replacement) Service engineer
Flowcell In case of leaking/obstruction or strong Service engineer
coloration

5.3.4 Replacement of peristaltic pump tube

1. Aspirate air to make sure all tubes are dry and will not leak upon disconnection.
2. Turn OFF the CYANSmart analyzer.
3. Lift the handle to open the door on the side to the peristaltic pump.
4. To change the tube, remove it from peristaltic pump rotor (2) and disconnect the pump tube from its
2 connectors by gently pulling them out. Insert the connectors in the new tube and place the new tube
(1) around the peristaltic pump rotor (2).
5. Make sure to insert the tubes in the correct direction. The tube coming from the flowcell (4) towards
the front and the tube going to the waste connector (5) towards the back.
6. Then close the hinged lock (3). Make sure it is completely clicked on.
7. To verify the fastening of the pump tube, manually turn the wheel of the peristaltic motor (correct
direction, see arrow). If the wheel can be turned smoothly, the tube is placed correctly. This manual
turning can only be performed when the instrument is turned OFF! When the instrument is turned ON,
the motor movements are blocked and manually turning the rotor can damage the mechanism!

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 ③

①
②

⑤ ④
③
OUT IN
To waste connector From flowcell
! ATTENTION POINTS!
1. Make sure the new pump tube is placed in the middle of the white rollers of the rotor.
2. Verify that the new tube is completely inserted into the holders.
3. Make sure the pump tube connectors are completely inserted into the new pump tube.
4. Make sure the tube (4) and (5), are inserted in the wholes and not squashed by the case.

√
X √
X

NOTE:
After replacing any part of the hydraulic circuit, you must recalibrate the peristaltic pump aspiration
volume! See 3.8.

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6 CONNECTION SOFTWARE
The connection software for the CYANSmart makes it very easy to view and process patient data on an
external computer and create patient reports to print on an external printer. Furthermore, you can also
easily check and update method programming. The software is user friendly and requires no training.
Although we advise you to go through this manual to completely understand the functionalities.

6.1 MINIMUM REQUIREMENTS FOR THE PC

 Memory 259 MB of free RAM memory
 Hard Disk Space 5 GB available
 Video Memory 4 Mb available
 Operating System Windows XP, Windows Vista, Windows 7
 Available USB A Port
 Languages available English, French

6.2 SOFTWARE INSTALLATION

If the CD with PC software was not yet included with the instrument, you can download it from our website
(you need to be registered first).

6.2.1 Adjustment of the settings on your computer

For the software to be able to correctly read and process values, the decimal symbol of your computer needs
to be set to a point. The procedure to check and adjust this setting depends on the windows version of your
computer.
6.2.1.1 Check your windows system

1. Click the Windows start button and choose Control Panel . In the window that

opens, choose classic view and click System

Or click right on the computer button on your desktop and select Properties

2. Your PC’s Windows System is visible in the window that opens.

6.2.1.2 Windows XP

1. Click the Windows start button and choose Control Panel

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2. In the window that opens, double click Regional and language settings
3. In the window that opens, select the Regional Options tab and click on Customize.
4. Adapt the decimal symbol from “,” (=comma) to “.” (=point), click apply and then ok.

6.2.1.3 Windows Vista & Windows 7

1. Click the Windows start button and choose Control Panel

2. In the window that opens, choose classic view/view by large icons and click Region and Language

.
If your view is set to “by Category” (upper right corner), go to “Clock, Language, and Region” and then
click “Change the date, time, or number format”.
3. In the window that opens, select the Formats tab and click on Additional settings.
4. Adapt the decimal symbol from “,” (=comma) to “.” (=point), click apply and ok.

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6.2.2 Installation of the CYANSmart software on your computer
1. Download and/or open the folder “CYANSmart_ENG” then “PC software”.
2. Double click on setup.exe in the setup folder.

3. If a security warning appears, click on <Run> to proceed.

4. Proceed with the installation by clicking <Next>.

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5. Select the folder to install the software on you C-drive: C:\Program Files (x86)\CYANSMART (by default)
and click on the <Next> button.
6. The software is at that time installed on the PC. You can cancel the installation at any time.

7. Click <Close> to finish the installation.

8. Please restart your computer to finalize the installation.

6.3 LAUNCH THE SOFTWARE

1. Start up the PC.
2. Make a connection between the computer and the CYANSmart by using a USB A-USB A cable.

3. Switch ON the CYANSmart.

4. Open the CYANSmart software by double clicking the icon or Start All Programs 
CYANSmart  CYANSmart.exe.

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5. To facilitate the opening of the software for the future, go to Start All Programs  CYANSmart
CYANSmart.exe and click right on CYANSmart.exe. Choose one of the following
i. Click Send to Desktop (create shortcut)
ii. Pin to Taskbar
iii. Pin to Start Menu

6.4 DESCRIPTION OF THE SOFTWARE

6.4.1 Home screen

When the software is successfully launched, the home screen will be displayed on your desktop.
In this home screen there are 3 different menus
(1) CYANSmart: To process patient data and modify or add methods.
(2) Quit: To exit the software.
(3) Status: Status of the connection between the CYANSmart and the computer.
o ‘CYANSmart is connected’ indicates a proper connection between the PC and the CYANSmart
o ‘CYANSmart is not connected’ indicates that the software doesn’t find the CYANSmart. Check
paragraph: ‘6.3. Launch the software’

6.4.2 Upload results from the CYANSmart to the software

When the blue status text ‘CYANSmart is connected’ is visible, it is possible to transfer data from the
CYANSmart to the software.
6.4.2.1 To transfer a single results stored in the CYANSmart to the software:
1. On the CYANSmart analyzer, select <Archives> and then click <Result list>.
2. Select the result you want to transfer.
3. Click <Data Synchronization> at the top right to transfer the result.

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6.4.2.2 To transfer all results stored in the CYANSmart to the software:
1. On the CYANSmart analyzer select <Archives> and then click <Data synchronization>.
2. Confirm you want to perform a <Data Synchronization>. All patient data on the CYANSmart are now
transferred to the software. Depending on the amount of data this can take just a few seconds or it can
take up to 3 minutes.

6.4.3 Manage patient results

In the PC software, click on <CYANSmart>  <Result> to open the screen where you can edit and view
patient data. The biochemical result view window is sub-divided into the following areas:
1. Selection criteria
2. Selected data
3. Patient information
4. Processing the data

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 1 3

Selection criteria This area can be used to visualize particular data/records. Data search can be done
using different criteria:
o Date of analysis (day of today as default)
o Age of the patient
o Sex of the patient
o Patient ID
o Name of the patient
o Reference of the patient (as inserted in the instrument)
After inserting all the information for the search of required data, click “Search” to
visualize all the results that corresponds to your search in the selected data area.
Note: There is an “AND” relation between the different fields. If more than 1 field
is filled in, the search can be narrowed down.
Selected data This area shows the data corresponding to your search. Upon selection of a result
in this area, the “Delete” and “Print” buttons and the “Patient information area”
become activated.
Patient information To edit or complete the patient information after selecting a record in the selected
data area. The ID field is read-only, all others can be modified. After changing
anything to a record: click ”Save”’ to confirm the change.
Print report title To adjust the heading of the patient report, write the correct title in the left bar.
Print (Ref) To print all results associated with a certain REF. See 6.4.4. for more details.
Delete To delete the result selected in the selected data area
Print To print the result selected in the selected data area on the printer set as default
for that computer.
Export to excel To export all the result to an excel file.

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6.4.4 Print patient reports
To create and print a patient report which includes all results associated with a certain reference.
Procedure:
1. Select the checkbox for “REF” and fill in the reference (number or name) of the patient for which you
want to create the patient report.
2. Click “Search”.
3. All results associated with the selected reference will be shown in chronological order and the button
“Print (Ref)” becomes activated.
4. Click “Print (Ref)”.

5. A window will pop-up were you can fill in the recommended patient information (if this was not yet
completed): Name, sex and age.

CYANSmart user manual, Version 20160902_(5.1) Page 66

6. Click “Print” to proceed with the printing of the patient report. All the tests performed for that patient
(with the same REF) will be mentioned on the list. At the bottom there is room for a ‘Signature of
authorization’. The patient report is automatically printed on the printer set as default for that computer.

6.4.5 Methods
This folder is protected by password. It contains settings intended solely to be examined or changed at the
factory or by a qualified technician, for example when a method programming update is available.
For accessing the method programming go to CYANSmart  Method setup. In this window you can easily
modify or update the methods settings on the CYANSmart itself.
ATTENTION: This menu should only be accessed by trained personnel. Changing methods can
influence the outcome of your results. Only change parameters of the methods when Cypress
Diagnostics instruct you to do so or launches an update of the application sheets.
Password: 999 and confirm by clicking ‘OK’.

CYANSmart user manual, Version 20160902_(5.1) Page 67

The method settings view is sub-divided into the following areas:
1. Overview of the programmed methods
2. Methods settings
3. Operating buttons
1 2

Method overview An overview and list of all programmed methods. Click the method to see the
details of the programmed parameters in the method settings area. To change
the order and thus the ID number, use the green buttons at the bottom.

or to move it one position up or down. to move it to the

first position or to move it to the last position.

Method settings The specific settings of a selected method. For detailed information on the
different parameters, please check the user manual Chapter 3.
The instrument is delivered with all Cypress Diagnostics biochemistry
methods already pre-programmed and ready to use. These optimized
parameters can always be reviewed in the application sheets (end of user
manual or website for the latest update). Changing methods can influence the
outcome of your results. Only change parameters of the methods when
Cypress Diagnostics instruct you to do so or launches an update of the
application sheets.
You can always include other methods of your choice. The instrument can
store 180 methods.
New To insert a new method. The new method will receive the first available ID
number. You can change it manually. Insert the method name (from 1 to 15
characters max). Then insert the parameters in the different areas (originally
default settings are shown). Finally, push Add.
Add To save a newly inserted method and add it to the list of programmed
methods.
Save To save changes made in the method settings for the method selected in the
method overview area.
CYANSmart user manual, Version 20160902_(5.1) Page 68
 Delete To delete the method selected in the method overview area.
Download method To retrieves the parameters from the CYANSmart analyzer for the selected
method
Upload method To send the parameters for the selected method towards the CYANSmart
analyzer.
Pay attention! This option will overwrite the existing method ID. We advise to
double check the methods after uploading them.
Download all methods To retrieves the parameters from the CYANSmart analyzer for all methods.
Upload all methods To send the parameters for all methods towards the CYANSmart analyzer.
Pay attention! This option will overwrite all existing methods. We advise to
double check the methods after uploading them.
Export To save all the methods with their settings as an Excel file on your PC.
Import To import methods from an Excel file in the CYANSmart connection software.

6.4.6 Methods update

Cypress Diagnostics guarantees that our reagents perform as described in the insert until expiration date
(as mentioned on the kit) if used and stored correctly.
When modification to the reagents are made to further improve the properties of the kit, some settings in
the method programming might need to be adjusted and the application sheets are updated. You can
always find the latest version of the application sheets on our website (http://www.diagnostics.be/). These
will also include the programming of all new kits. From our website you can also download the Excel file
with all method information to import in the software and upload to the CYANSmart analyzer (you need to
be registered). This file is provides as a zip file. Please extract it to obtain the Excel file.
Procedure:
1. Start up the PC, then switch ON (I) the CYANSmart, then open the CYANSmart software
2. Go to CYANSmart  Method setup.
3. Click <Import>.
4. A warning appears indicating that the original settings in the software will be replaced, click <OK>.

5. Select the correct Excel file with the latest settings supplied by Cypress Diagnostics or downloaded from
our website and click <Open>.

CYANSmart user manual, Version 20160902_(5.1) Page 69

6. When the import towards the software is successfully executed the following pop-up appears. Press
<OK>. Now all the method settings are successfully transferred from the Excel file to the software.

7. Click <Upload all methods> to transfer the method settings from the software to the CYANSmart
analyzer. A status bar will appear:

8. When the upload to the CYANSmart is successfully completed the following pop-up appears. Press
<OK>.

Verify that the update was successful by going to Methods  Program List on the CYANSmart and verify
the method programming with the latest application sheets.

CYANSmart user manual, Version 20160902_(5.1) Page 70

7 TROUBLESHOOTING

7.1 OPERATIONAL
Trouble Diagnosing and solution:
AD Zero out of range Check if:
for all filters - The instrument is ON for at least 30 min.
- The lamp is ON.
 If the lamp is off: contact a service engineer.
- The flowcell is in the correct direction (windows to front and back).
- The flowcell is completely inserted to the bottom of the flowcell holder.
- The optical cover is closed during measurement.
- The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
 Perform washing and rinsing (5.2).
- The windows of the flowcell are clean (no dirt/fingerprints on the outside).
 Clean with wipes for glasses.
- The flowcell is filled with distilled water and without any air bubbles.
 Verify the pump calibration and the absence of leakages, cracks in tubes & flowcell.
- The gain of all/most filters is 15, 16, are more.
 The lamp is aged and should be replaced: contact a service engineer.
If after these verifications, the problems remain, please contact a service engineer.
Check (by service engineer):
- Lamp and replace if necessary.
- Sensor of filter wheel.
AD Zero out of range Check if:
for one or few filters - The instrument is ON for at least 30 min.
- The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
 Perform washing and rinsing (5.2).
- The windows of the flowcell are clean (no dirt/fingerprints on the outside).
 Clean with wipes for glasses.
- Verify the 2 points above even more thorough if (only) the 340 nm filter is out of range,
because this filter is the most sensitive to dirt are insufficient rinsing.
- The flowcell is filled with distilled water and without any air bubbles.
 Verify the pump calibration and the absence of leakages, cracks in tubes & flowcell.
If after these verifications, the problems remain, please contact a service engineer.
Check (by service engineer):
- Sensor of filter wheel
- Filter
OD values are near Check if:
zero - If the lamp is ON for at least 30 min.
 If the lamp is off: contact a service engineer.
- The flowcell is in the correct direction (windows to front and back).
- The flowcell is completely inserted to the bottom of the flowcell holder.
- The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
 Perform washing and rinsing (5.2).
- The windows of the flowcell are clean (no dirt/fingerprints on the outside).
 Clean with wipes for glasses.
- The flowcell is filled with distilled water and without any air bubbles.
 Verify the pump calibration and the absence of leakages, cracks in tubes & flowcell.
- The reagents are deteriorated (reagent blank values out of range).
- Preparation of the (working reagent) and the samples.
- The method has been programmed correctly.
If after these verifications, the problems remain, please contact a service engineer.

CYANSmart user manual, Version 20160902_(5.1) Page 71

 The concentrations Check if
are zero - The OD values are (near) zero.
 See point above.
- Restart the measurement and include a (new) calibration!
 The first time you perform a method, it is essential that you perform a calibration,
so the instrument can determine the factor for the calculation of the concentration.
 Check the application sheets if a standard, calibrator or factor should be used.
Some standard and (almost) all calibrators have a lot dependent concentration! Make sure
the correct concentration is programmed in the methods!
Blank is out of range Check if:
- The instrument is ON for at least 30 min.
- The method programming is consistent with the latest application sheets: distilled water
or reagent, the number of blank measurements and the blank limits.
- The flowcell is filled with distilled water and without any air bubbles.
 Verify the pump calibration and the absence of leakages, cracks in tubes & flowcell.
- Blank is distilled water.
 Perform washing and rinsing (5.2), then restart the test.
- Blank is reagent.
 Verify the preparation of the (working) reagent
 Reagent is deteriorated, check storage conditions (expiration date and opening
date), open new kit or vial and restart the test.
If after these verifications, the problems remain, please contact a service engineer.
QC is out of range or Check if:
bad accuracy of - The instrument is ON for at least 30 min.
results - Reagent is deteriorated, check storage conditions (expiration date and opening date)
 Open new kit or vial.
- The preparation instructions of the (working reagent), blank, (standard) and the controls
have been followed strictly: stability, volumes, times (application sheets).
- The method programming is consistent with latest application sheets.
Quality controls have a lot dependent concentration! Make sure the correct range is
programmed in the methods!
- The flowcell is filled with distilled water and without any air bubbles.
 Verify the pump calibration and the absence of leakages, cracks in tubes & flowcell.
- Restart the measurement and include a new calibration!
o Check the application sheets if a standard, calibrator or factor should be used.
o Some standard and (almost) all calibrators have a lot dependent concentration!
Make sure the correct concentration is programmed in the methods!
o Some methods do not have a standard included in the kit. For these methods, a factor
is pre-programmed. You can use this factor if the QC measurements are within range.
However, for the most accurate results, it is recommended to use a calibrator (see
application sheets) to calculate the factor. This requires adjusting the settings of the
method; program NUM of STD: 1 and CONC: as mentioned on the insert/vial.
o If even after recalibration with the standard included in the kit, the QC measurements
are still outside the range, it is recommended to use a calibrator to perform a more
accurate calibration (see application sheets).  Adjust method programming!
If after these verifications, the problems remain, please contact a service engineer.

CYANSmart user manual, Version 20160902_(5.1) Page 72

 Bad repeatability of Check if:
results - No power problem (see below).
- If the instrument is ON for at least 30 min.
- The optical cover is closed during measurement.
- The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water.
 Perform washing and rinsing (5.2).
- The flowcell is filled with distilled water and without any air bubbles.
 Verify the pump calibration and the absence of leakages.
- The reagents are not deteriorated.
- The pipette for the manual handling has been correctly calibrated and the tips are
compatible.
If after these verifications, the problems remain, please contact a service engineer.

7.2 TECHNICAL
Trouble Diagnosing and solution:
Power problem Check if:
- The original power cord supplied with the instrument is used.
- The connection of the power is correct and firm.
- The fuses are not burned.
 Replace with the correct fuses: 2x T2AL250V.
- Placed on a stable, horizontal surface, free from vibrations.
- Placed away from other appliances that generate electrical noise or magnetic fields (e.g.
radiological instruments, X-ray, radio…).
- Connected to a suited and grounded AC wall outlet, preferably one that is not shared
with other electric appliances and with low fluctuation of line voltage:
AC 220V ± 10%, 50Hz ± 2% or AC 110V ± 10%, 60Hz ± 2%.
- If the AC wall outlet is not properly grounded, please connect a ground or earth wire to
the bottom plate of the analyzer.
- If the fluctuation is >10% use of the following supplementary devices is strongly
recommended:
 Electronic Stabilizer.
 No Break module UPS – (Uninterrupted Power Supply).
After turning off the analyzer, you need to wait at least 60 seconds before
turning it on again! Otherwise, the fuses will blow because of the residual
current.
If after these verifications, the problems remain, please contact a service engineer.
NOTE :
In case of an electric shock, the parameters saved on the mainboard can be modified or
deleted. Please ask the technician of your distributor to check and if necessary readjust the
programming methods, the pump calibration, the temperature calibration and the other
settings.
Temperature Check if:
problem - Not in direct sunlight, this could affect the operating temperature.
- Not in a draught or under/up/ beside air conditioning or heat sources.
- Leave 10 cm around and at the back of the instrument for air circulation and cooling.
- Room temperature between 15 – 30 °C.
- Instrument is on for at least 15 - 30 minutes.
- In the temp display (system settings):
- The measured temperature for the flowcell is the set temperature ±1°C.
- The measured temperature for the incubator is the set temperature 37.0 ±1°C.
If after these verifications, the measured temperatures do not correspond to these
specifications, contact a service engineer.

CYANSmart user manual, Version 20160902_(5.1) Page 73

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 Aspiration problem Check if:
- The hydraulic system (flowcell + tubes) is clean and rinsed with distilled water (5.2).
- Verify that after aspiration of distilled water, there is no big drop formation at the tip of
the aspiration tube. Drop formation indicates the presence of leakage or blockage in the
hydraulic system (tubes or flowcell):
- Leak in one of the tubes or the flowcell:
 Replacement is necessary, contact a service engineer.
- Blockage in one of the tubes can be caused by:
 Squashing of the tube, for example by the case, replace the tube if damaged,
contact a service engineer.
 Sticking together of the pump tube. To prevent this, it is recommended to unhook
the pump tube if the instrument is not used for more than 1 week.
 Accumulation of dirt in the tubes  perform a cleaning with hypochlorite (5.2),
replace if cleaning is not sufficient.
- Blockage in the flowcell:
 Accumulation of dirt in the flowcell  perform a cleaning with hypochlorite (5.2),
replace if cleaning is not sufficient.
- The pump tube is installed and connected correctly (2.3.3).
- The peristaltic pump is correctly calibrated.
- The peristaltic pump motor is turning smoothly.
If after these verifications, the problems remain, please contact a service engineer.
Printing problem Check (user):
- There is thermal paper inserted.
- The paper is inserted in the correct direction (prints only on one side).
- The result print is enabled in the print setup.
If after these verifications, the problems remain, please contact a service engineer.
Screen is not Check (user):
readable - Touch the screen to exit the standby mode.
- Adjust the screen brightness in the system setup (3.12.9).
If after these verifications, the problems remain, please contact a service engineer.
System test failed If one of the tests fails, the System Test presents an error. Besides this general error, the
instrument will indicate what part of the instrument causes the error. E.g. AD convert,
Printer, Motor or sensor of filter wheel...
Please contact a service engineer.

CYANSmart user manual, Version 20160902_(5.1) Page 74

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
8 SAFETY INFORMATION

This information summarizes the established guidelines for handling laboratory biohazard. Use this
summary for general information only. It is not intended to replace or supplement your laboratory’s or
hospital’s biohazard control procedures.
By definition, a biohazard condition is a situation involving infectious agents biological in nature, such as
the hepatitis B virus, the human immunodeficiency virus, and the tuberculosis bacterium. These infectious
agents may be present in human blood, blood products and other body fluids.

The following are the major sources of contamination when handling potentially infectious agents:
 Needles.
 Hand-to-mouth contact.
 Hand-to-eye contact.
 Direct contact with superficial cuts, open wounds and other skin conditions that may permit adsorption
into subcutaneous skin layers.
 Splashes or aerosol contact with skin and eyes.

To prevent accidental contamination in a clinical laboratory, strictly adhere to the following procedures:
 Wear gloves while servicing parts of the system that have been in contact with body fluids such as
serum, plasma, urine, or whole blood.
 Wash your hands before going from a contaminated area to a non-contaminated area, or when you
remove or change gloves.
 Perform procedures carefully to minimize aerosol formation.
 Wear facial protection when splatter or aerosol formation are possible.
 Wear personal protective equipment such as safety glasses, gloves, lab coats or aprons when working
with possible biohazard contaminants.
 Keep your hands away from your face.
 Cover all superficial cuts and wounds before starting any work.
 Dispose contaminated materials according to your laboratory’s biohazard control procedures.
 Keep your work area disinfected.
 Disinfect tools and other items that have been near any part of the system sample path or waste area
with 10% v/v bleach.
 Do not eat, drink, smoke, or apply cosmetics or contact lenses while in the laboratory.
 Do not mouth pipet any liquid, including water.
 Do not place tools or any other items in your mouth.
 Do not use the biohazard sink for personal cleaning such as rinsing coffee cups or washing hands.
 Do not recap, purposely bend, cut, break, remove from disposable syringes, or otherwise manipulate
needles by hand.

CYANSmart user manual, Version 20160902_(5.1) Page 75

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
INSTALLATION FORM
Please FILL IN the INSTALLATION FORM and send a COPY BACK TO CYPRESS DIAGNOSTICS
Make sure to register also the instrument on the website: www.diagnostics.be

Distributor (Company name)

(Country, city)
(E-mail)
Coordinates
(Website)
(Tel.)
(Name)
Technician in charge (E-mail)
(Tel.)

Laboratory (Laboratory name)

(Country, city)
(E-mail)
Coordinates
(Website)
(Tel.)
(Name)
Lab technician (E-mail)
(Tel.)

Delivery date (date)

Installation date (date)
Instrument CYANSmart End warranty (date)
Serial number
Software version

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
Checklist for installation (for detailed explanation see manual)

□ Check content according to packing list

□ Check location requirements

□ Check electronical current requirements. If necessary, use:
□ Electronic stabilizer
□ UPS
Installation of all accessories on the CYANSmart analyser

□ Connect the power cord to a suited power supply

□ Install the peristaltic pump

□ Install the flowcell, aspiration button and aspiration tube

□ Install the waste tube and the waste bottle

□ Switch on the instrument

□ Install printing paper

Checking the CYANSmart analyser

□ Check system self-test at start-up

□ Perform washing and cleaning

□ Check the aspiration and hydraulic circuit by performing a pump calibration

□ Check the optical system by performing an AD autozero

Adaptation of the system to the customer

□ System setup: print setup, standby time, date and time, hospital name, language

□ Customize the methods, adjust the units if necessary

□ User manual and application sheets in laboratory

□ Give extensive information on daily use and maintenance to the user

Comments

Laboratory technician
Name
Signature
Maintenance engineer
Name
Signature
Date

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
MAINTENANCE FORM
Distributor (Company name)
(Country, city)
(E-mail)
Coordinates
(Website)
(Tel.)
(Name)
Technician in charge (E-mail)
(Tel.)

Laboratory (Laboratory name)

(Country, city)
(E-mail)
Coordinates
(Website)
(Tel.)
(Name)
Lab technician (E-mail)
(Tel.)

Delivery date (date)

Installation date (date)
Instrument CYANSmart End warranty (date)
Serial number
Software version
Last visit: (date)
Next visit: (date)

Laboratory technician
Name
Signature
Maintenance engineer
Name
Signature
Date

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
MAINTENANCE INSTRUCTIONS
To be performed by Period Activity Date Date Date Date Date
AD autozero
Rinse between methods
Wash the flowcell with detergent
Daily
Laboratory Clean outside of the instrument
Technician
Use dust cover to protect the instrument after
shutdown
Disinfect the flowcell with hypochlorite
Weekly
Pump calibration
Remove dust inside the instrument
6 months
Replace the peristaltic pump tube (CY009-S25)
1 year/ 2000
Replace the lamp (CY009-S11)
hours
Replace:
 Plastic aspiration tube (CY004/9-S26)
 Waste tube (CY004/9-S28)
1 year
 Tube from pump to waste connection
Maintenance (CY004/9-S40)
Engineer  Tube from flowcell to pump (CY004/9-S41)

Replace:
 Pre-amplifier board with cable (CY009-S33)
together with lamp
2/3 years  Rubbers of peristaltic pump (CY009-S54)
together with the pump tube
 Connector of waste tube (CY009-S29)
 Connector of pump tube (CY009-S30)

Problems Fill in repair form*

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium • www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 Version: December 2016
Valid till: next update
Page: 1

CYANSmart CONSUMABLES
Item Code Item name Photo Comments Consumable Suggested stock:
for one year* 10 sold instruments

Accessories & Consumables

CY001-S01 Thermal print paper (57mm width) Y user dependent user dependent

CY001-WS CYAN Washing solution Y 4 5

CY004/9-S43 Dust cover N 2

Hydraulic & aspiration assembly

CY009-S54 Rubber of peristaltitic pump (2 pcs) Replace every 2-3 years (Y) 6

Replace every 6 months

CY009-S25 Pump tube Y 2 10
See hydraulic diagram

Replace every year

CY004/9-S26 Plastic aspiration tube Y 1 5
See hydraulic diagram

CY004/9-S27 Metal aspiration tube See hydraulic diagram N 3

Replace every year

CY004/9-S28 Waste tube Y 1 5
See hydraulic diagram

Replace every 2-3 years

CY009-S29 Connector of waste tube (Y) 2
See hydraulic diagram

Replace every 2-3 years

CY009-S30 Connector of pump tube (2 pcs) (Y) 4
See hydraulic diagram

Replace every year

CY004/9-S40 Tube from pump to waste connector Y 1 5
See hydraulic diagram

Replace every year

CY004/9-S41 Tube from flow cell to pump Y 1 5
See hydraulic diagram

CY004/9-S44 Waste bottle (Y) 3

Optic Assembly

CY004/9-S09 Flowcell (Y) 2

Replace every 2000 hours/year

CY009-S11 Lamp (with frame, cable and connector) Y 1 5
Connection directly on mainboard (6V 10W)

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 Version: December 2016
Valid till: next update
Page: 2

Item Code Item name Photo Comments Consumable Suggested stock:

for one year* 10 sold instruments

CY009-S33 Pre-amplifier board with cable Replace every 2-3 years (Y) 5

Power Assembly

CF-T2.0AL250V Fuse T2AL250V (10 pcs) 2 per instrument (Y) 1

Hydraulic diagram

Cypress Diagnostics: Nijverheidsstraat 8 • 2235 Hulshout • Belgium

www.diagnostics.be • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
 CY009

English
To all Healthcare Professionals,

Thank you for your interest. We appreciate it!

CYANSmart
Cypress Diagnostics provides the tools and solutions that
let clinical laboratories worldwide deliver clear and precise
diagnostic results.
We manufacture the equipment needed for handling the

Semi-Automatic Biochemistry
samples, analyzers to perform the tests, reagents to carry out
the tests and - increasingly often - the software platforms to
report the results.

Analyzer Cypress Diagnostics supplies clinical laboratories.

We are a quality-oriented company with ISO certification

and CE-approved products. Our production facility is located
in Hulshout, Belgium. Our products are preferred and
appreciated by users in more than 100 countries worldwide.
Cypress Diagnostics is a family-owned business, founded in
1995 in Leuven, Belgium.

We work exclusively with selected and trained distributors.

We sell high-quality instruments and customer service is an
integral part of our quality policy.
All our products come with an aftersales and performance
guarantee.

Please feel free to contact our local distributor for more

information.
Our latest inserts and catalogs are downloadable on
www.diagnostics.be .

Test the difference!

Kind regards,

The Cypress Diagnostics Team

ISO 13485-2016
www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]
CYANSmart Technical Specifications

Optical system Power supply

The CYANSmart is an an easy-to-operate semi-automatic biochemistry analyzer. This new in vitro diagnostic • Flow cell: 32 µl, 10 mm light path • AC 110/220 V - 50/60 Hz
instrument is designed to have powerful features in a compact, independent unit. • Min. reaction volume: 500 µl per test • Automatic voltage and frequency switch
• Halogen lamp 6V/10W • Grounding required
• Photo detector: Silicon based (range 300 – 900 nm) • Power: 200 VA, reduced to 140 VA in standby mode
Cost savings: Convenience:
• Measurement range: 0,000 – 3,500 Abs • Power save / Standby mode
• Minimum reaction volume of 500 µl means decreased • Easy tracking of all the results from each patient by a
• Wavelength: 340 – 620 nm
costs per test unique identifier
• Wavelength selection: Automatic via 7 interference Software
• Limited consumables required • All Cypress Diagnostics biochemistry reagents are
filters: 340, 405, 492, 510, 546, 578 & 620 nm • Unique 16 digit patient ID
• Extra items included on delivery (two fuses, five paper preprogrammed on delivery
• Memory: 180 test methods and 6000 sample test results
rolls, a spare lamp, and pump tube) • 180 open channels are programmable to allow for
Flow cell • Cypress Diagnostics methods preprogrammed on
• Integrated incubator, printer, pump, and flow cell other user-defined tests
• 25 °C, 30 °C, 37 °C, ambient temperature delivery
• Long life lamp, further extended by power saving / • Memory for up to 6000 sample results
• Peltier element • Languages: English, French, Spanish
standby mode • Large font size, easily legible and read with little effort
• Thermostatic control: PID controlled, ±0,5 °C • QC: 2 controls programmable plus a separate QC results
• Robustness of the analyzer with minimal daily • LCD touch screen with virtual alphanumeric keyboard
• 10 mm menu with (printable) graphs
maintenance • Multilingual capability
• Calculation methods: absorbance, endpoint, two-point,
• Analyzer guides the user and so reduces mistakes • Minimal daily maintenance
Incubator kinetic, bichromatic
• 20 places with 14 mm diameter • Calibration methods: factor, calibrator and multi-point
Flexibility:
• 37 °C calibration
• On-board printer for printing automatically or on Desktop Software:
• Thermostatic control: PID controlled, ± 1 °C • Blank options: sample blank, reagent blank and water
request printing • Clear reporting with patient overview with flags and
• Incubation time: 0 – 999 seconds
• Seven wavelengths (340, 405, 492, 510, 546, 578, and reference ranges
Display • Reading time: 3 – 999 seconds
620 nm filters) cover all clinical diagnostic applications • Easier reporting and result sharing (email & network
• Back-illuminated LCD
• Five calculation methods (absorbance, endpoint, printing)
• Touchscreen calibration possible Weight and dimensions
two-point, kinetic, bichromatic) and three calibration • Elimination of transcription errors (no more manual
• 800 x 480 pixels (screen size: 7 inch) • Instrument: 9 kg, 20 x 43,5 x 41,5 cm (H x W x L)
methods (factor, calibrator, and multi-point data entry)
calibration) provide plenty processing options with • Data back-up
Printer Environmental requirements
one system • Convenient upgrade of the methods by quick upload
• Automatic or on-demand printing • Ambient temperature: 15 °C – 30 °C
• Three blank options (sample blank, reagent blank, and • Built-in thermal printer • Relative humidity: 30 % - 70 %
water) provide background correction of the reagent • 24 characters per line
and sample coloration • Prints graphs Order code:
• CY009: CYANSmart

Contact: e-mail: [email protected] Your Distributor

tel: + 32 (0)15 67 67 68
Nijverheidsstraat 8
2235 Hulshout
Belgium
URL: www.diagnostics.be CYANSmart EN 2019-04

ISO 13485-2016 ISO 13485-2016

www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected] www.diagnostics.be • Belgium • Tel: ++ 32 15 67 67 68 • e-mail: [email protected]