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One-pot synthesis of amides from carboxylic acids

activated using thionyl chloride†
Cite this: RSC Adv., 2016, 6, 34468
A. Leggio,*a E. L. Belsito,a G. De Luca,b M. L. Di Gioia,a V. Leotta,a E. Romio,a
C. Sicilianoa and A. Liguori*a

A one-pot synthesis of secondary and tertiary amides from carboxylic acids and amines by using SOCl2 has
been developed. Also when sterically hindered amines were used as the starting materials, excellent yields
Received 19th November 2015
Accepted 29th March 2016
of the corresponding amides were obtained. The amidation of N-protected a-amino acids with secondary
amines proceeds effectively with good yields. The process works well also in the presence of acid sensitive
DOI: 10.1039/c5ra24527c
groups and occurs with almost complete retention of stereochemical integrity of chiral substrates. This
www.rsc.org/advances protocol could be extended to industrial large-scale production processes.

tetramethyl-a-chloroenamine14 which gives rise to neutral side-

Introduction products and bis(trichloromethyl)carbonate (BTC).15
Amides represent one of the most important and valuable Thionyl chloride (SOCl2) is the most popular reagent to
organic functional groups in naturally occurring molecules, activate carboxylic function because it is volatile and the excess
pharmaceuticals, agrochemicals, and polymers.1 can be removed easily by distillation, nally it is non-expensive.
The synthesis of amide is of huge importance in organic, Usually, for the preparation of acid chlorides, thionyl chlo-
coordination, and medicinal chemistry.2 ride is used, neat or dissolved in a solvent, in the presence of the
Improved and innovative methods for the synthesis of corresponding acid. The reaction requires heating and can be
amides are in great demand both by chemical and pharma- accelerated by adding pyridine.16 Improvements of the classical
ceutical industry.3–5 SOCl2–pyridine method was achieved by forming the acid
Carboxylic amides are typically obtained from amines and chlorides from dicyclohexylammonium salts of carboxylic acids
activated carboxylic acid derivatives through a nucleophilic acyl and thionyl chloride.17
substitution reaction. Acid derivatives most frequently used in Secondary and tertiary amides were also obtained in a one pot
the amides synthesis are acyl chlorides. reaction by treating carboxylic acids with thionyl chloride and
The amidation of carboxylic acids via acyl chlorides is usually stoichiometric amounts of amines in the absence of base and in
a two-step process,6 involving rst the conversion of the acid the presence of N,N-disubstituted amides ((N,N-dimethylaceta-
into the acyl chloride followed by the coupling itself with the mide (DMAC), N,N-dimethylformamide (DMF), N-methyl-
amine. pyrrolidone (NMP)).18–24 The reaction occurs by generating the
Chlorination of carboxylic acids is carried out using several Vilsmeier complex from N,N-disubstituted amides (DMAC, DMF,
chlorinating reagents, such as pivaloyl chloride, phthaloyl NMP) and thionyl chloride that acts as reagent for chlorination
dichloride, thionyl chloride and oxalyl chloride.7–10 of carboxylic acids. The formation of amides is completed in
Other chlorinating reagents11 that can be employed in amide more than 1 hour and in some cases, to achieve 100% conver-
formation are 2,4,6-trichloro-1,3,5-triazine (CC, cyanuric chlo- sion, it is necessary to heat to 50
C.18 In other cases instead, the
ride),12 2-chloro-4,6-dimethoxy-1,3,5-triazine (CDMT)13 and reaction takes places in longer times and with low yields.20,21
In Table 1 is reported a comparison of different one pot
thionyl chloride methods for amide synthesis.
a
Dipartimento di Farmacia e Scienze della Salute e della Nutrizione, Università della As an alternative, the carboxyl function activation can be
Calabria Edicio Polifunzionale, I-87036 Arcavacata di Rende, CS, Italy. E-mail: performed by using coupling agents26 that are also widely used
[email protected]; [email protected]; Fax: +39 0984 493265; Tel: for the formation of peptide bond.27–29 They allow to overcome
+39 0984 493205; +39 0984 493199
b
side reactions such as ketene formation, acyl transfer and to
Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P.
reduce racemization in peptide coupling.
Bucci, I-87036 Arcavacata di Rende, Italy
† Electronic supplementary information (ESI) available: 1H and 13C NMR spectra
Here we report the use of thionyl chloride as activating agent
for compounds 1–20. 13C NMR spectra for elucidation of reaction mechanism, of carboxylic function for the one-pot synthesis of amides.
HPLC analyses of compounds 16, 17 and of a mixture of 16 and 17. Chiral The thionyl chloride (SOCl2), used as a chlorinating agent in
GC/MS analyses of amides 18, 19 and a mixture of 18 and 19. See DOI: the formation of acyl chlorides, could also function as coupling
10.1039/c5ra24527c

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Table 1 Comparison between different one-pot thionyl chloride injection port was heated to 250
C. The oven temperature
methods for amide synthesis program was initially set at 50
C ramped to 200
C at 0.5
C
min1 with a hold of 20 min.
ESI-QTOF mass spectra were recorded on a Quadrupole Time
of Flight (QTOF) mass spectrometer tted with an electrospray
ionization source (ESI) operating in positive ion mode.
Analytical RP-HPLC analyses were carried out using a C18
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RP analytical column (Tecnocroma Tracer Excel 120, ODSA,

Entry Reaction conditions SOCl2 (equiv.) Yield (%) Time Ref.
15  0.4 cm, 5 mm) with an elution gradient from 20% to 40%
1 DMACa, 20 to 50
C 1.08 80–90 >1 h 18 of B over 10 minutes, from 40% to 50% of B in 20 minutes,
2 DMACa, 15 to 20
C n.r. 92 n.r.c 19 followed by 50–60% of B in 10 minutes, A being H2O
3 DMACa, 12 to 20
C 1.27 45 9h 20 and B CH3CN and detection at 254 nm. Flow rate was 1
4 DMACa, 20 to 20
C 1.04 51 3.5 h 21
mL min1.
5 NMPa, 0 to 25
C 1.04 85 1.5 h 22
6 DMFb, Et3N, 45
C 1.05 69 3h 23
7 Toluenea, 40–70
C 1.20 n.r. 2–24 h 25
General procedure for one pot synthesis of amides 1–19
a
Reaction solvent. b 0.05 equiv. c n.r. ¼ not reported.
1 mmol of carboxylic acid is added to 1 mmol of amine and 3
mmol of triethylamine (Et3N) in dichloromethane, then 1 mmol
of SOCl2 is added at room temperature. The mixture is stirred
agent by activating in situ the carboxylic function of the acid.
for 5–20 minutes at room temperature. The recovery of the
Then the amine present in the reaction medium could react
reaction product is performed by evaporating the solvent under
with the reactive intermediate by generating the amide, and as
reduced pressure. The resulting residue is taken up in
a result, the reaction would proceed under one-pot conditions.
dichloromethane and washed rst with 1 N HCl* and then with
1 N NaOH. The organic phase was dried (Na2SO4), and evapo-
Experimental rated to dryness to afford the corresponding carboxylic amide.
*For the synthesis of N,N-diethylamides of N-Boc-protected
Materials and methods amino acids (15, 18 and 19) the acidic work up was performed
Reagents were commercially available with analytical grade and by using 5% aqueous NaHSO4.
used as purchased without further purication. All a-amino N,N-Diethylbenzamide (1). Viscous red oil, 86%, Rf ¼ 0.77,
acids were of the L-series. Solvents were puried according to TLC (eluent: diethyl ether/P.E. 70 : 30 v/v): 1H NMR (300 MHz,
well-known laboratory methods and freshly distilled prior to CDCl3) d: 7.43–7.34 (m, 5H, ArH), 3.63–3.43 (m, 2H, NCH2),
use. All reactions were carried out using ame-dried glassware 3.38–3.18 (m, 2H, NCH2), 1.32–1.18 (m, 3H, CH2CH3) 1.17–1.02
and under an inert atmosphere (dry N2). Reaction mixtures were (m, 3H, CH2CH3); 13C NMR (75 MHz, CDCl3) d: 171.3, 137.2,
monitored by thin layer chromatography (TLC) using silica gel 129.1, 128.4, 126.2, 43.3, 39.2, 14.2, 12.9. MS (EI, 70 eV) m/z (%
60-F254 pre-coated glass plates. Spots on the TLC plates were rel.): 177 [M+c] (20), 176 (49), 162 (8), 148 (16), 105 (100), 77 (48),
visualized with a UV lamp (254 nm) and by spraying with 0.2% 51 (21). Anal. calcd for C11H15NO: C, 74.54; H, 8.53; N, 7.90.
ninhydrin in ethanol and charring aer elution. 1H and 13C Found: C, 74.65; H, 8.51; N, 7.88.
NMR spectra were recorded at 300 MHz and 75 MHz, respec- N,N-Diethylpyrazine-2-carboxamide (2). Viscous colorless
tively. Spectroscopic analysis was performed at 293 K on diluted oil, 88%, TLC (eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.39;
1
solutions of each compound by using CDCl3 as the solvent. H NMR (300 MHz, CDCl3) d: 8.83–8.78 (m, 1H, ArH), 8.56–8.50
Chemical shis (d) are reported in ppm and referenced to CDCl3 (m, 1H, ArH), 8.49–8.43 (m, 1H, ArH), 3.49 (q, J ¼ 6.5 Hz, 2H,
(singlet at 7.25 ppm for 1H and 77.0 ppm, central line of the NCH2), 3.31 (q, J ¼ 6.5 Hz, 2H, NCH2), 1.19 (t, J ¼ 6.5 Hz, 3H,
triplet, for 13C spectra). Coupling constants (J) are reported in CH2CH3), 1.08 (t, J ¼ 6.5 Hz, 3H, CH2CH3); 13C NMR (75 MHz,
hertz (Hz). CDCl3) d: 166.1, 150.2, 144.9, 144.7, 142.5, 43.2, 40.4, 14.2, 12.6.
The 1H, 13C spectra of compounds 18, 19 and 20 were GC/MS: m/z 179 [M+c] (5), 107 (30), 79 (35), 72 (100). Anal. calcd
recorded at 298 k on a Bruker Avance 500 MHz instrument (1H: for C9H13N3O: C, 60.32; H, 7.31; N, 23.45. Found: C, 60.40; H,
500.13 MHz, 13C: 125.77 MHz). 7.29; N, 23.51.
GC-MS analyses were performed using a 30 m  0.25 mm, N,N-Diethyl-3-phenylbenzamide (3). Viscous colorless oil,
PhMesiloxane capillary column. The mass detector was oper- 91%, TLC (eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.75; 1H
ated in the electron impact ionization mode (EIMS) with an NMR (300 MHz, CDCl3) d: 7.50–7.23 (m, 9H, ArH), 3.85–3.70 (m,
electron energy of 70 eV. The injection port was heated to 250 1H, NCH2), 3.10–2.89 (m, 2H, NCH2), 2.71–2.52 (m, 1H, NCH2),


C. The oven temperature program was initially set at 100
C 0.89 (t, J ¼ 6.8 Hz, 3H, CH2CH3), 0.74 (t, J ¼ 6.8 Hz, 3H,
with a hold of 2 min and ramped to 280
C at 14
C min1 with CH2CH3); 13C NMR (75 MHz, CDCl3) d: 170.7, 139.7, 138.4,
a hold of 10 min. 136.1, 128.9, 128.6, 128.5, 128.3, 127.5, 126.8, 42.3, 38.4, 13.3,
Chiral GC-MS analyses of enantiomeric compounds 18 and 11.9; MS (EI, 70 eV) m/z (% rel.): 253 [M+c] (37), 252 (75), 181
19 were performed by using a 25 m  0.25 mm, diethyl tert- (100), 152 (51), 77 (38). Anal. calcd for C17H19NO: C, 80.60; H,
butyldimethylisilyl-b-cyclodextrine chiral capillary column. The 7.56; N, 5.53. Found: C, 80.72; H, 7.58; N, 5.54.

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N,N-Diethylcinnamamide (4). Viscous colorless oil, 86%, rel.): 239 [M+c] (55), 238 (40), 210 (10), 148 (5), 134 (6), 105 (100),
TLC (eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.55; 1H NMR 91 (20), 77 (35). Anal. calcd for C16H17NO: C, 80.30; H, 7.16; N,
(300 MHz, CDCl3) d: 7.73 (d, J ¼ 15.3 Hz, 1H, CH vinyl), 7.61– 5.85. Found: C, 80.41; H, 7.17; N, 5.86.
7.50 (m, 2H, ArH), 7.40–7.34 (m. 3H, ArH), 6.84 (d, J ¼ 15.3 Hz, N-Propylbenzamide (10). Viscous colorless oil, 92%, TLC
1H, CH vinyl), 3.60–3.40 (m, 4H, NCH2), 1.33–1.15 (m, 6H, (eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.38, 1H NMR (300
CH2CH3) ppm; 13C NMR (75 MHz, CDCl3) d: 165.7, 142.3, 135.5, MHz, CDCl3) d: 7.95–7.70 (m, 2H, ArH), 7.51–7.28 (m, 3H, ArH),
129.4, 128.7, 127.7, 117.7, 42.3, 41.1, 15.1, 13.2 ppm; MS (EI, 70 3.36 (t, J ¼ 7.2 Hz, 2H, NHCH2), 1.72–1.54 (m, 2H, NHCH2CH2),
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eV) m/z (% rel.): 203 [M+c] (31), 188 (20), 131 (100), 103 (40), 77 0.90 (t, J ¼ 7.2, Hz, 3H, CH3CH2); 13C NMR (75 MHz, CDCl3) d:
(23). Anal. calcd for C13H17NO: C, 76.81; H, 8.43; N, 6.89. Found: 168.9, 131.9, 129.9, 128.5, 127.4, 42.3, 22.5, 11.3; MS (EI, 70 eV)
C, 76.76; H, 8.41; N, 6.90. m/z (% rel.): 163 [M+c] (40), 148 (5), 134 (10), 105 (100), 77 (40).
N,N-Diethylpalmitamide (5). Viscous colorless oil, 90%, TLC Anal. calcd for C10H13NO: C, 73.59; H, 8.03; N, 8.58. Found: C,
(eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.68; 1H NMR (300 73.69; H, 8.04; N, 8.59.
MHz, CDCl3) d: 3.42–3.20 (m, 4H, NCH2), 2.35 (t, J ¼ 7.2 Hz, 2H, N-Phenylbenzamide (11). Viscous colorless oil, 89%, TLC
CH2CO), 1.67–1.50 (m, 2H, CH2CH2CO), 1.34–1.10 (m, 30H, (eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.58, 1H NMR (300
CH3(CH2)12CH2CH2CO and (CH3CH2)2N), 0.81 (t, J ¼ 6.6 Hz, 3H, MHz, CDCl3) d: 8.35–7.05 (m, 11H, ArH and NH), 13C NMR (75
(CH2)14CH3); 13C NMR (75 MHz, CDCl3) d: 173.8, 34.2, 32.5, 31.8, MHz, CDCl3) d: 165.9, 137.9, 133.2, 130.1, 129.0, 128.7, 127.1,
29.6, 29.4, 29.3, 29.2, 25.7, 24.8, 22.6, 14.0; MS (EI, 70 eV) m/z (% 124.3, 120.3 MS (EI, 70 eV) m/z (% rel.): 197 [M+c] (60), 105 (100),
rel.): 311 [M+c] (12), 128 (25), 115 (100), 100 (28), 72 (16). Anal. 77 (50). Anal. calcd for C13H11NO: C, 79.16; H, 5.62; N, 7.10.
calcd for C20H41NO: C, 77.10; H, 13.26; N, 4.50. Found: C, 77.18; Found: C, 79.27; H, 5.63; N, 7.11.
H, 13.29; N, 4.48. N,N-Diethyl-(S)-2-(4-nitrophenylsulfonamido)-3-phenylpro-
N,N-Diethylmyristamide (6). Viscous colorless oil, 88%, TLC panamide (12). Pale yellow oil, 81%, TLC (eluent: chloroform/
(eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.67, 1H NMR (300 methanol 90 : 10 v/v): Rf ¼ 0.62; 1H NMR (300 MHz, CDCl3) d:
MHz, CDCl3) d: 3.38 (q, J ¼ 7.2 Hz, 2H, NCH2), 3.31 (q, J ¼ 7.2 8.22 (d, J ¼ 9.0 Hz, 2H), 7.88 (d, J ¼ 9.0 Hz, 2H, ArH), 7.25–7.15
Hz, 2H, NCH2), 2.29 (t, J ¼ 7.2 Hz, 2H, CH2CO), 1.70–1.59 (m, (m, 3H, ArH), 7.14–7.04 (m, 2H, ArH), 6.24 (d, J ¼ 8.1 Hz, 1H,
4H, (CH2)2CH2CO), 1.38–1.23 (m, 18H, CH3(CH2)9(CH2)2- NH), 4.48–4.32 (m, 1H, CHCO), 3.39–3.23 (m, 1H, CH2CH3),
CH2CO), 1.18 (t, J ¼ 7.2 Hz, 3H, CH2CH3), 1.12 (t, J ¼ 7.2 Hz, 3H, 3.09–2.83 (m, 5H, CH2Ph and NCH2), 0.99–0.80 (m, 6H,
NCH2CH3), 0.89 (t, J ¼ 6.6 Hz, 3H, CH3(CH2)12CO); 13C NMR (75 CH2CH3); 13C NMR (75 MHz, CDCl3) d: 169.3, 149.8, 146.3,
MHz, CDCl3) d: 172.2, 41.9, 39.9, 33.1, 31.9, 29.6, 29.5, 29.4, 29.3, 135.4, 129.5, 128.6, 128.2, 127.3, 123.9, 54.5, 41.5, 40.7, 13.9,
25.5, 22.6, 14.4, 14.1, 13.1; MS (EI, 70 eV) m/z (% rel.): 283 [M+c] 12.5; MS (EI, 70 eV) m/z (% rel.): 405 [M+c] (2), 314 (51), 305
(12), 128 (25), 115 (100), 100 (28), 72 (16). Anal. calcd for (100), 203 (44), 100 (55), 72 (61). Anal. calcd for C19H23N3O5S:
C18H37NO: C, 76.26; H, 13.16; N, 4.94. Found: C, 76.37; H, 13.27; C, 56.28; H, 5.72; N, 10.36; S, 7.91. Found: C, 56.43; H, 5.74; N,
N, 4.95. 10.33; S, 7.88.
N-Ethyl-N-isopropylbenzamide (7). Viscous colorless oil, N,N-Diethyl-(S)-2-(4-nitrophenylsulfonamido)-propanamide
86%, TLC (eluent: diethyl ether/P.E. 70/30 v/v): Rf ¼ 0.71; 1H (13). Pale yellow oil, 83%, TLC (eluent: chloroform/methanol
NMR (300 MHz, CDCl3) d: 7.50–7.20 (m, 5H, ArH), 4.10–3.80 (m, 90 : 10 v/v): Rf ¼ 0.72; 1H NMR (300 MHz, CDCl3) d: 8.32 (d, J
1H, NCH), 3.52–3.25 (m, 2H, NCH2), 1.39–0.95 (m, 9H, ¼ 8.1 Hz, 2H, ArH), 8.04 (d, J ¼ 8.1 Hz, 2H, ArH), 6.24 (d, J ¼ 8.7
CH(CH3)2 and CH2CH3); 13C NMR (75 MHz, CDCl3) d: 171.1, Hz, 1H, NH), 4.32–4.18 (m, 1H, CHCO), 3.30–3.05 (m, 4H,
129.9, 128.9, 128.4, 125.9, 50.2, 35.2, 21.1, 14.8; m/z (% rel.): NCH2), 1.33 (d, J ¼ 6.9 Hz, 3H, CHCH3), 1.09 (t, J ¼ Hz, 3H,
[M+c] 191 (22), 190 (25), 176 (5), 162 (10), 148 (5), 105 (100), 77 CH2CH3), 0.92 (t, J ¼ Hz, 3H, CH2CH3); 13C NMR (75 MHz,
(35). Anal. calcd for C12H17NO: C, 75.35; H, 8.96; N, 7.32. Found: CDCl3) d: 170.1, 149.9, 146.3, 128.4, 124.1, 49.2, 41.6, 40.6, 20.5,
C, 75.48; H, 8.94; N, 7.31. 14.3, 12.6; MS (EI, 70 eV) m/z (% rel.): 229 (69), 186 (15), 122
N-Benzoylpiperidine (8). Viscous colorless oil, 88%, TLC (10), 100 (95), 72 (100). Anal. calcd for C13H19N3O5S: C, 47.41;
(eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.50; 1H NMR (300 H, 5.81; N, 12.76; S, 9.74. Found: C, 47.55; H, 5.82; N, 12.80; S,
MHz, CDCl3) d: 7.29–7.15 (m, 5H, ArH), 3.65–3.45 (m, 2H, 9.76.
NCH2), 3.28–3.10 (m, 2H, NCH2), 1.58–1.27 (m, 6H, 4-Acetamidophenylacetate (14). Viscous colorless oil, 48%,
NCH2(CH2)3); 13C NMR (75 MHz, CDCl3) d: 170.1, 136.3, 129.2, TLC (eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.88; 1H NMR
128.2, 126.6, 48.5, 42.9, 26.3, 25.5, 24.4; MS (EI, 70 eV) m/z (% (300 MHz, CDCl3) d: 7.91 (s, 1H, NH), 7.55 (d, J ¼ 9.0 Hz, 2H,
rel.): 189 [M+c] (35), 188 (100), 105 (75), 77 (41). Anal. calcd for ArH), 7.01 (d, J ¼ 9.0 Hz, 2H, ArH), 2.29 (s, 3H, CH3CO), 2.17 (s,
C12H15NO: C, 76.16; H, 7.99; N, 7.40. Found: C, 76.29; H, 8.01; N, 3H, CH3CO), 13C NMR (75 MHz, CDCl3) d 169.8, 168.6, 147.0,
7.41. 135.9, 121.8, 120.8, 24.4, 21.1; MS (EI, 70 eV) m/z (% rel.): 193
N-Benzyl-N-ethylbenzamide (9). Viscous colorless oil, 85%, [M+c] (10), 151 (55), 109 (100), 80 (10). Anal. calcd for C10H11NO3:
TLC (eluent: diethyl ether/P.E. 70 : 30 v/v): Rf ¼ 0.59; 1H NMR C, 62.17; H, 5.74; N, 7.25. Found: C, 62.26; H, 5.75; N, 7.26.
(300 MHz, CDCl3) d: 7.60–6.90 (m, 10H, ArH), 4.90–4.42 (m, 2H, N,N-Diethyl-(S)-2-(tert-butoxycarbonyl)-amino-3-phenylpro-
NCH2Ph), 3.78–3.05 (m, 2H, NCH2), 1.38–1.02 (m, 3H, CH2CH3); panamide (15). Pale yellow oil, 78%, TLC (eluent: diethyl ether/
13
C NMR (75 MHz, CDCl3) d:170.8, 133.4, 130.1, 129.8, 128.9, P.E. 70 : 30 v/v): Rf ¼ 0.81; 1H NMR (300 MHz, CDCl3) d: 7.26–
128.5, 128.3, 127.6, 126.6, 53.9, 42.9, 13.9; MS (EI, 70 eV) m/z (% 7.10 (m, 5H, ArH), 5.39 (d, J ¼ 9 Hz, 1H, NH), 4.74–4.64 (m, 1H,
CHCO), 3.53–3.41 (m, 1H, CH2CH3); 3.11–2.80 (m, 5H CH2Ph,

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CH2CH3), 1.37 (s, 9H, tBu), 0.99 (t, J ¼ 7.2 Hz, 3H, CH3CH2N), eV) m/z (% rel.): 270 [M+c] (1), 197 (19), 170 (40), 114 (94), 100
0.92 (t, J ¼ 7.2 Hz, 3H, CH3CH2N), 13C NMR (75 MHz, CDCl3) (30), 70 (100), 57 (45). Anal. calcd for C14H26N2O3: C, 62.19; H,
d 170.8, 154.9, 136.5, 129.5, 128.3, 126.7, 79.5, 51.3, 41.5, 40.4, 9.69; N, 10.36. Found: C, 62.23; H, 9.63; N, 10.31.
28.2, 14.0; MS (EI, 70 eV) m/z (% rel.): 320 [M+c] (2), 229 (6), 220
(20), 164 (45), 129 (40), 120 (100), 100 (35), 91 (20), 72 (40), 57
Synthesis of dipeptide methyl 3-methyl-2-(S)-(2-(R)-(4-
(70). Anal. calcd for C18H28N2O3: C, 67.47; H, 8.81; N, 8.74.
nitrophenylsulfonamido)-propanamido)butanoate (20)
Found: C, 67.51; H, 8.87; N, 8.75.
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N-((R)-1-Phenylethyl)-2-(S)-(4-nitrophenylsulfonamido)-pro- 1 mmol of 2-(S)-(4-nitrophenylsulfonamido)propanoic acid is

panamide (16). Pale yellow oil, 75%, TLC (eluent: diethyl ether/ added to 1 mmol of (S)-methyl 2-amino-3-methylbutanoate
P.E. 70 : 30 v/v): Rf ¼ 0.88; 1H NMR (300 MHz, CDCl3) d: 8.17 (d, hydrochloride and 5 mmol of triethylamine (Et3N) in dichloro-
J ¼ 8.7 Hz, 2H, ArH), 7.93 (d, J ¼ 8.7 Hz, 2H, ArH), 7.34–7.23 (m, methane, then 1 mmol of SOCl2 is added at room temperature.
5H, ArH), 5.94 (d, J ¼ 8.2, 1H, NH), 5.55 (d, J ¼ 8.1, 1H, NH), The mixture is stirred for 40 minutes at room temperature. The
4.97–4.86 (m, 1H, CHPh), 3.90–3.79 (m, 1H, CHCO), 1.44 (d, J ¼ recovery of the reaction product is performed by evaporating the
6.9 Hz, 3H, CH3CHPh), 1.39 (d, J ¼ 6.9 Hz, 3H, CH3CHCO); 13C solvent under reduced pressure. The resulting residue is taken
NMR (75 MHz, CDCl3) d 168.1, 152.1, 142.1, 137.9, 128.8, 128.2, up in dichloromethane and washed rst with 1 N HCl and then
127.8, 125.9, 124.3, 55.1, 49.7, 21.8, 20.1; anal. calcd for with 1 N NaOH. The organic phase was dried (Na2SO4), and
C17H19N3O5S: C, 54.10; H, 5.07; N, 11.13; S, 8.50. Found: C, evaporated to dryness to afford the corresponding dipeptide 20.
54.18; H, 5.15; N, 11.28; S, 8.61. ESI-QTOF-MS: 378.1063 (M + White solid, 65%; mp 144–148
C; TLC (eluent: chloroform/
H)+, 400.0944 (M + Na)+. methanol 90 : 10 v/v): Rf ¼ 0.53; 1H NMR (500 MHz, CDCl3) d:
N-((S)-1-Phenylethyl)-2-(S)-(4-nitrophenylsulfonamido)-pro- d 8.27 (d, J ¼ 8.8 Hz, 2H, ArH), 8.00 (d, J ¼ 8.8 Hz, 2H, ArH), 6.47
panamide (17). Pale yellow oil, 68%, TLC (eluent: diethyl ether/ (d, J ¼ 8.7 Hz, 1H, NHSO2ArH), 5.93 (d, J ¼ 8.5 Hz,
P.E. 70 : 30 v/v): Rf ¼ 0.88; 1H NMR (300 MHz, CDCl3) d: 8.35 (d, 1H,NHCHCOOCH3), 4.31 (dd, J ¼ 8.5 Hz, J ¼ 4.8 Hz, 1H,
J ¼ 9 Hz, 2H, ArH), 8.05 (d, J ¼ 9 Hz, 2H, ArH), 7.38–7.21 (m, CHCOOCH3), 4.02–3.91 (m, 1H, NHCH(CH3)CO, 3.69 (s, 3H,
5H, ArH), 5.97 (d, J ¼ 7.2 Hz 1H, NH), 5.60 (sbroad, 1H, NH), OCH3), 2.12–2.04 (m, 1H, CH(CH3)2), 1.33 (d, J ¼ 7.1 Hz, 3H,
4.98–4.88 (m, 1H, CHPh), 3.91–3.82 (m, 1H, CHCO), 1.40 (d, J ¼ CH3CH), 0.84 (d, J ¼ 6.9 Hz, 3H, CH(CH3)2), 0.80 (t, J ¼ 6.9 Hz,
6.9 Hz, 3H, CH3CHPh), 1.35 (d, J ¼ 6.9 Hz, 3H, CH3CHCO); 13C 3H, CH(CH3)2). 13C NMR (126 MHz, CDCl3) d 172.31, 171.13,
NMR (75 MHz, CDCl3) d 168.1, 151.8, 141.2, 136.9, 128.8, 128.4, 150.35, 145.75, 128.68, 124.60, 57.22, 52.88, 52.61, 31.47, 20.40,
127.8, 125.9, 124.3, 52.5, 49.1, 22.8, 21.1; anal. calcd for 19.07, 17.70; MS (EI, 70 eV) m/z (% rel.): 328 [M+c  COOMe]
C17H19N3O5S: C, 54.10; H, 5.07; N, 11.13; S, 8.50. Found: C, (28), 274 (8), 229 (100), 186 (27), 130 (27), 122 (21), 72 (14). Anal.
54.15; H, 5.09; N, 11.23; S, 8.59. ESI-QTOF-MS: 378.1092 (M + calcd for C15H21N3O7S: C, 46.50; H, 5.46; N, 10.85; S, 8.28.
H)+, 400.0939 (M + Na)+. Found: C, 46.38; H, 5.48; N, 10.90; S, 8.26.
1-(tert-Butoxycarbonyl)-2-(S)-(N,N-diethylaminocarbonyl)-
pyrrolidine (18). Pale yellow oil, 75%, TLC (eluent: diethyl
ether/P.E. 70 : 30 v/v): Rf ¼ 0.78; 1H NMR (500 MHz, CDCl3) Results and discussion
two conformers (60*:40) d: 4.55 (dd, J ¼ 7.8 Hz, J ¼ 2.9 Hz, 1H, In order to verify the one-pot formation of the amide by using
CHCO), 4.41* (dd, J ¼ 8.4, J ¼ 3.8 Hz, 1H, CHCO), 3.63–3.15 thionyl chloride as activating agent, we designed an experiment
(m, 6H, CH2CH2NCO, CH2CH3), 2.21–1.91 (m, 2H, CH2CH2- in which benzoic acid, the carboxylic acid chosen as model
CHCO), 1.87–1.71 (m, 2H, CH2CH2CHCO), 1.41 and 1.47* (2 system, is treated, in the presence of triethylamine, with thionyl
s, 9H, tBu), 1.24–1.15 (m, 3H, CH3CH2N), 1.13–1.03 (m, 3H, chloride and the amine (Scheme 1).
CH3CH2N); 13C NMR (126 MHz, CDCl3) d 172.06, 154.62, 1 mmol of benzoic acid was added to 1 mmol of diethyl-
154.07*, 79.63*, 79.33, 56.57*, 56.32, 47.08, 46.94*, 41.84, amine (Et2NH) and 3 mmol of triethylamine (Et3N) in
41.05*, 40.84, 31.35*, 30.37, 28.62, 28.57*, 24.29, 23.53*, dichloromethane, then 1 mmol of SOCl2 was added.
14.85, 13.26*, 13.04; MS (EI, 70 eV) m/z (% rel.): 270 [M+c] (1), Aer 5 minutes stirring at room temperature, TLC analysis of
197 (18), 170 (37), 114 (88), 100 (29), 70 (100), 57 (43). Anal. the reaction mixture showed the complete conversion of ben-
calcd for C14H26N2O3: C, 62.19; H, 9.69; N, 10.36. Found: C, zoic acid.
62.25; H, 9.65; N, 10.30. The recovery of the reaction product was performed by
1-(tert-Butoxycarbonyl)-2-(R)-(N,N-diethylaminocarbonyl)- evaporating the solvent under reduced pressure to remove
pyrrolidine (19). Pale yellow oil, 73%, TLC (eluent: diethyl traces of unreacted thionyl chloride. The resulting residue, aer
ether/P.E. 70 : 30 v/v): Rf ¼ 0.78; 1H NMR (500 MHz, CDCl3)
two conformers (59*:41) d: 4.53 (dd, J ¼ 7.8 Hz, J ¼ 3.0 Hz, 1H,
CHCO), 4.38* (dd, J ¼ 8.4 Hz, J ¼ 3.8 Hz, 1H, CHCO), 3.60–
3.11 (m, 6H, CH2CH2NCO, CH2CH3), 2.19–1.90 (m, 2H, CH2-
CH2CHCO), 1.86–1.68 (m, 2H, CH2CH2CHCO), 1.38 and 1.34*
(2s, 9H, tBu), 1.22–1.13 (m, 3H, CH3CH2N), 1.10–1.01 (m, 3H,
CH3CH2N); 13C NMR (126 MHz, CDCl3) d 172.10, 154.60,
154.03*, 79.53*, 79.28, 56.62*, 56.35, 46.95, 41.78, 40.96, Scheme 1 One pot synthesis of amides from carboxylic acids using
31.34*, 30.31, 28.58, 24.27, 23.44*, 14.76, 13.14; MS (EI, 70 SOCl2 and Et3N.

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Table 2 Results of one pot synthesis of amides under the reaction Table 2 (Contd. )
conditions reported in Scheme 1
Product Reaction time (min) Yielda (%)
Product Reaction time (min) Yielda (%)

20 40 65
1 5 86
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a
Yields based on starting amount of carboxylic acid.
2 5 88

3 5 91
work up, provided the corresponding N,N-diethylbenzamide (1)
in 86% overall yield (Table 2).
The molecular structure of 1 was assigned by 1H and 13C
4 5 86 NMR spectroscopy and GC/MS analysis.
An additional experiment was performed to investigate the
reaction progress in absence of the tertiary amine Et3N. To this
5 5 90 aim 1 mmol of SOCl2 was added to 1 mmol of benzoic acid and 1
mmol of diethylamine (Et2NH) in dichloromethane at room
temperature. Aer 20 minutes the reaction was not yet complete
6 5 88 and a mixture of benzoic acid (65% yield) and N,N-dieth-
ylbenzamide (31% yield) was recovered.
The use of stoichiometric amounts of diethylamine, in
7 5 86 absence of Et3N, leads to a lower conversion to amides since the
diethylamine works also as a base to neutralize the hydrochloric
acid that is generated during the reaction. This result demon-
8 5 88
strated that the presence of the tertiary amine is essential to
obtain the amide in high yields.
We also experienced that the outcome of the reaction is
9 5 85
strongly dependent on the order of reagent addition. In fact, if
benzoic acid is preliminarily added to thionyl chloride and
10 5 92 Et2NH and Et3N are added subsequently, the reaction yield is
lowered and aer 5 minutes, the reaction is not yet complete.
The obtained reaction product contained also the benzoyl
11 5 89
chloride with a percentage of 35% as detected by GC/MS
analysis.
12 20 81 Furthermore, it was veried that SOCl2 does not react with
the amine. In fact, the N-benzyl-N-ethylamine was recovered
unchanged aer it was treated with SOCl2 at room temperature
13 20 83 for 1 hour.
With the aim of investigating the reaction mechanism, we
14 15 48 designed 13C NMR experiments by performing and monitoring
the reactions directly into the NMR tube using CDCl3 as solvent
and observing the chemical shis of the carbonyl groups of the
15 15 78
products present in the reaction mixture.
The purpose of these experiments was to identify the reactive
16 20 75 intermediate generated during the one pot amide formation
reaction. Therefore, we recorded preliminarily the 13C NMR
spectra of benzoic acid and benzoyl chloride that exhibit
17 20 68
carbonyl resonance signals respectively at 172.51 ppm and
168.38 ppm.
18 16 75 Subsequently in a NMR tube, benzoic acid (1 mmol) was
treated with thionyl chloride (1 mmol) at room temperature in
CDCl3 as solvent. The progress of the reaction was followed by
19 15 73 recording the 13C NMR spectrum of the reaction mixture. Aer
more than 2 hours 13C NMR spectrum showed the presence of
the signal at (172.44 ppm) corresponding to the carbonyl group
of benzoic acid and a signal of very low intensity, that can be

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just appreciated, relating to acyl chloride carbonyl group are similar to those of the reaction with diethylamine (Table 2,
(168.52 ppm) This demonstrated that benzoyl chloride is 7–9).
formed hardly under these reaction conditions. Then, with the purpose to investigate the steric hindrance
In an additional experiment, the same reaction was per- present on the carboxylic acid, the developed procedure was
formed at room temperature by adding thionyl chloride (1 applied to two N-protected a-amino acids, N-nosyl-L-phenylala-
mmol) to a mixture of benzoic acid (1 mmol) and triethylamine nine and N-nosyl-L-alanine.
(3 mmol) dissolved in CDCl3. In this case the reaction proceeds N-Nosyl-L-phenylalanine and N-nosyl-L-alanine were con-
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rapidly and in the 13C NMR spectrum it was observed imme- verted into the corresponding N,N-diethylamides (Table 2, 12,
diately the disappearance of the signal relating to the resonance 13) in 20 minutes and with yields slightly lower.
of benzoic acid carbonyl group and the appearance at 168.13 Based on these results it can be argued that steric hindrance
ppm of the signal generated by benzoyl chloride carbonyl group. offered by the groups on the nitrogen atom of the amine does
Finally, we reproduced, the one pot formation of N,N-dieth- not affect the reaction progress while the reaction times are
ylbenzamide (1) in a NMR tube, under the described conditions longer and the reaction yields are a little lower when sterically
for the synthesis of amides 1–17, and immediately recorded the hindered carboxylic acids are used.
13
C NMR spectrum. In the obtained 13C NMR spectrum at once The adopted procedure for obtaining N,N-diethylamides was
the presence of two resonances attributable to the carbonyl also applied successfully to N-Boc-L-phenylalanine, a substrate
groups of N,N-diethylbenzamide at 171.36 ppm and of benzoyl bearing the acid labile group tert-butoxycarbonyl (Boc). The
chloride at 168.32 ppm was observed. Aer about 10 minutes, corresponding N,N-diethylamide (15) was obtained in 78% yield
the re-recorded 13C NMR spectrum of the reaction mixture keeping unchanged the acid-sensitive Boc group (Table 2). On
revealed only the presence of the resonance corresponding to the contrary the application of the two-step amide synthesis,
the amidic carbonyl group. through the formation of acyl chloride, to N-Boc-L-phenylala-
These results could be justied by the action of tertiary nine does not work well due to the instability of the corre-
amine that causes the formation of the carboxylate, which sponding acyl chloride that, when isolated in the absence of the
reacts quickly at room temperature with thionyl chloride to nucleophile, decomposes readily.
form the acid chloride that immediately reacts with the N,N- The described protocol was also employed to obtain
diethylamine to give the corresponding amide. secondary amides by using primary amines as nucleophilic
This experimental evidence suggests that benzoyl chloride is reagents. To this aim, N-propylamine and aniline were selected
the reactive intermediate of the adopted one pot procedure as nucleophiles. The reactions afforded the corresponding
(Scheme 2). amides in short times and high yields (Table 2, 10, 11) by
The reaction was subsequently extended to other aromatic demonstrating that the adopted one-pot procedure is also
substrates, in all experimented cases the reaction is complete applicable successfully for obtaining secondary amides.
within 5 minutes and has provided excellent yields (86–91%) We also investigated the stereochemical aspects of the
(Table 2, 2–4). reaction by extending the developed procedure to the formation
The methodology was then applied to two long chain of a couple of diastereoisomeric amides. To this aim N-((R)-1-
aliphatic carboxylic acids, palmitic and myristic acid. Also with phenylethyl)-2-(S)-(4-nitrophenylsulfonamido)-propanamide
these systems no changes were observed during the reaction, in (16) and N-((S)-1-phenylethyl)-2-(S)-(4-nitrophenylsulfonamido)
fact the amide formation occurs even in short times and high propanamide (17) were synthesized by treating N-(4-nitro-
yields (88–90%) (Table 2, 5, 6). benzenesulfonyl)-L-alanine with (R)-1-phenylethylamine and (S)-
In order to evaluate the possible effects due to the steric 1-phenylethylamine respectively according the adopted condi-
hindrance of the amine, N-ethyl-N-isopropylamine, piperidine tions. The corresponding amides 16 and 17 were obtained in
and N-benzyl-N-ethylamine were treated with benzoic acid 68–75% yields aer 20 minutes (Table 2). The 1H NMR and 13C
under the described reaction conditions. NMR spectroscopic data of crude amides 16 and 17 did not
Signicant effects due to steric hindrance of the amines were show any signals from possible epimers resulting from an
not detected. Both the reaction kinetics that the reaction yields inversion of the conguration at the a-carbon atom of the
alanine.
The absence of epimerization was also monitored by HPLC
analysis of the crude amides 16 and 17 and of a suitably
prepared mixture of the two diastereoisomers 16 and 17
(approx. 30% : 70%). The two diastereoisomers were readily
resolved by HPLC: the presence of two chromatographic peaks
with retention times of 15.558 and 16.867 minutes was observed
in the HPLC analysis of the mixture (Fig. 1B). Chromatograms
of the single amide 16 (Fig. 1A) and 17 (Fig. 1C) showed the
presence of a unique peak with retention times of 16.908 and
15.433 minutes respectively.
HPLC analysis in combination with NMR data of the two
Scheme 2 Proposed mechanism of the one pot amide synthesis. diastereomeric amides 16 and 17, demonstrated the absence of

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temperature. The reaction, monitored by TLC (Et2O/petroleum

ether, 7 : 3, v/v), was complete aer 15 minutes and provided
the N,N-diethylbenzamide (1) in 78% yield. This experiment
showed that our protocol can be also applied successfully to
synthesis of amides on a multi-gram scale.
Finally, we evaluated the chemoselectivity of the reaction by
using a nucleophile containing both an amino and a phenolic
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function. According to the developed procedure, p-amino

phenol was treated with an equimolar amount of acetic acid and
thionyl chloride. Aer 15 minutes, TLC analysis (Et2O/
petroleum ether, 7 : 3, v/v) showed the complete conversion of
the starting material. The reaction workup afforded p-acet-
amidophenyl acetate in 48% yield (Table 2, 14). Therefore, the
acylation reaction takes place both on the amino and phenolic
function without any chemoselectivity.

Conclusions
Fig. 1 HPLC analyses of crude amides 16 and 17: (A) N-((R)-1-phe- Thionyl chloride works as coupling reagent and allows obtain-
nylethyl)-2-(S)-(4-nitrophenylsulfonamido)-propanamide (16); (B) ing, at room temperature and in short times, secondary and
a mixture of 16 and 17 (approx. 30% : 70%); (C) N-((S)-1-phenylethyl)-
tertiary amides in high yields without need of further purica-
2-(S)-(4-nitrophenylsulfonamido)-propanamide (17).
tion. Furthermore, the adopted conditions did not cause any
signicant loss of the optical integrity at the chiral centres of the
precursors as conrmed by HPLC and NMR analysis of a couple
epimerization products excluding any detectable epimerization
of synthesized diastereoisomeric amides and by the high
throughout the synthetic process.
enantiomeric excess estimated for N,N-diethylamides of N-Boc-
Furthermore, in order to evaluate the enantiomeric purity of
D-proline.
N,N-diethylamides of N-Boc protected a-amino acids, two N,N-
The reaction progress is affected by steric hindrance only
diethylamides of N-Boc-L-proline (18) and its enantiomer N-Boc-
when bulky groups are present on the carboxylic acid molecule.
D-proline (19) were synthesized using the developed protocol
The synthesis of amides is performed in an efficient one-pot
(Table 2) and characterized by chiral GC/MS analysis.
procedure under mild reaction conditions; the order of
The separation of L-amide 18 and D-amide 19 was achieved by
reagent addition is predetermined for obtaining the amides in
chiral GC/MS analysis of a mixture of the two enantiomers 18
high yields.
and 19 (approx. 70% : 30%): the corresponding chromatogram
The reaction mechanism was investigated by performing 13C
showed the presence of two peaks with retention times of
NMR experiments, which showed, as the reactive intermediate
114.08 and 114.73 minutes related to 18 and 19 respectively.
is the acyl chloride. This is formed easily from the carboxylate
The chiral GC/MS analysis of the single amide 18 showed
generated in situ from the corresponding carboxylic acid in the
a unique peak at 113.97 min. While that of the single amide 19
presence of the tertiary amine.
showed two peaks at 113.88 and 114.75 minutes which corre-
Our approach was successfully extended to a-amino acids
sponded to identical mass spectra. The estimated enantiomeric
bearing acid-sensitive groups and to the synthesis of a dipeptide
excess of D-amide 19 was 99.2%. The resulting ee was really
system; these results prove the applicative validity of the
satisfactory.
developed procedure also in the peptide synthesis.
For completeness, since the amidation is mainly used in
Finally, the reaction can be also applied for large-scale
peptide synthesis, the developed protocol was also experi-
synthesis of amides demonstrating its applicability for amide
mented for the synthesis of a small peptide. For this purpose
syntheses of preparative and semi-industrial interest.
the dipeptide system methyl 3-methyl-2-(S)-(2-(R)-(4-
nitrophenylsulfonamido)-propanamido)butanoate (20) was
prepared by treating N-4-nitrophenylsulfonyl-D-alanine (1 Acknowledgements
mmol) with L-valine methyl ester hydrochloride (1 mmol) under
This work was supported by grants from MIUR (Ministero Ital-
the adopted reaction conditions using 5 equivalents of Et3N.
iano dell'Università e della Ricerca, ex-60% funds).
The methodology was successful in giving, aer 40 minutes, the
desired dipeptide derivative 20, which was isolated in good
yields (65%, Table 2). References
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