™

BACTEC  9050
System User’s
Manual


2004/09 Becton, Dickinson and Company
Document Number MA–0103 7 Loveton Circle
Revision: E Sparks, Maryland 21152


445845 1–800–638–8656


BENEX Limited
Bay K 1a/d
BACTEC™ 9050 System User’s Manual

Change History
REVISION DATE PAGES RE A S O N

New 4/96 All New product release

i – ii, v – vi, 7 – 10, 13 – 14, Minor additions to

A 8/96
53 – 58, 77 – 78, 87 – 88 clarify service issues

Software version 1.10;

B 12 / 9 6 All cabinet interlock &
EROs

Software version 1.21;

C 7//97 i, ii, 11 – 12, 51 – 52
add heat spec.

i, ii, 11 – 20, 29 – 30, Add required info. for

D 6/98
51 – 52 Myco/F Lytic medium

E 2004/09 All General updates

This product is covered by one or more of

o f the following patents:
US 5,266,486
US 5,518,923

BACTEC is a registered trademark of Becton, Dickinson and Company.

© Copyright Becton, Dickinson and Company, 1996 – 2004. All rights

reserved. No part of this publication may be reproduced, transmitted,
transcribed, stored in retrieval systems, or translated into any language
 u Preface

Contents

1 - Introduction
1.1 BACTEC
BACTE C 9050
9050 Overvie
Overview w .....
........
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
... 1
1.2 Instrumen
Instrumentt Overview
Overview .... ........
........
.........
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.....33
1.3 Softwa
Software
re Overvi
Overview ew ......
.........
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
... 5
1.3.1
1.3.1 Built-in-T
Built-in-Test
est (Patent
(Patent Pending)
Pending) .... .........
.........
.........
.........
........
........
........
.........
........
... 6
1.4 Manual Structur
Structure.....
e.........
.........
.........
.........
.........
........
.........
.........
.........
.........
........
........
........
.........
.........
......
.. 7
1.5 Use of this Manual
Manual ......
.........
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
...88
1.6 Conventions
Conventions ....
.........
.........
.........
.........
.........
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
........
........
.... 9
1.6.1
1.6.1 General....
General........
........
........
.........
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.........
......
.. 9
1.6.2
1.6.2 Symbols
Symbols Used on the the Equipment..
Equipment....... .........
........
........
........
.........
.........
.......
... 10
1.6.3
1.6.3 Notes,
Notes, Cautions,
Cautions, and Warnings Warnings .... ........
........
........
........
........
........
.........
.........
.... 10
1.7 Summar
Summaryy of Warnings
Warnings and Cautio Cautions ns ......
.........
......
......
......
......
......
......
......
......
......
......
..... 11
2 - Installation and Setup
2.1 Genera
Generall Requir
Requireme
ementsnts ......
.........
......
......
.....
.....
......
......
......
......
......
......
......
......
......
......
......
......
......
......
... 15
2.2 Instrum
Instrument
ent Specifi
Specificatcation
ionss .....
........
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
......
..... 16
2.3 Installati
Installation
on .........
..............
.........
........
........
........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.... 18
2.4 Instrumen
Instrumentt Setup.....
Setup..................
.........
.........
.........
........
........
........
........
.........
.........
.........
.........
........
........
...... 18
2.4.1
2.4.1 General....
General.........
.........
.........
.........
.........
.........
........
........
........
........
.........
.........
.........
.........
.........
.........
.... 18
2.4.2
2.4.2 Setup
Setup Parameters
Parameters .........
.............
.........
.........
.........
.........
........
........
........
.........
.........
.........
....... 19
2.5 System
System Start-up
Start-up ....
........
.........
.........
.........
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
....... 23
3 - Controls and Indicators
3.1 General......
General...........
.........
........
........
........
.........
.........
........
.........
.........
........
........
........
.........
.........
.........
.........
.........
....... 25
3.2 On/Off Switch
Switch............
.........
.........
.........
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.... 27
3.3 Keypad
Keypad and LCDLCD Displa
Displayy ......
.........
......
.....
.....
......
......
......
......
......
......
......
......
......
......
......
......
......
......
... 27
3.3.1
3.3.1 SILENCE
SILENCE ALARM Key .... ........
........
.........
.........
.........
.........
........
........
........
.........
.........
.......
... 28
3.3.2
3.3.2 HOME ROTOR Key .... ........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.... 28
3.3.3
3.3.3 Alarm Indicator
Indicator .........
..............
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
.......
... 29
3.3.4
3.3.4 Brightness
Brightness Dial .... .........
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.... 29
3.3.5
3.3.5 Soft Keys
Keys ....
.........
.........
.........
.........
........
.........
.........
.........
.........
.........
.........
........
........
........
.........
....... 29
3.3.6
3.3.6 New Positi
Positive ve Indicator.....
Indicator......... .........
.........
........
........
........
.........
.........
.........
.........
.......
... 29
3.3.7
3.3.7 DOWN/UP
DOWN/UP ARROW Keys....... Keys............
.........
.........
.........
.........
.........
.........
.........
.........
....... 30
3.3.8
3.3.8 Display
Display Area .... ........
.........
.........
........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.........
.... 30
BACTEC™ 9050 System User’s Manual

Contents

3.6 Barcode Scanner....................................................................... 31

3.7 Audible Tones and Alarms ........................................................ 32
4 - Operation
4.1 General..................................................................................... 33
4.2 Preparing Specimens ............................................................... 34
4.2.1 Media Quality Control ................................................... 34
4.3 Daily Checks ............................................................................. 35
4.4 Entering New Vials ................................................................... 35
4.5 Positive and Negative Specimens.............................................. 39
4.5.1 General.......................................................................... 39
4.5.2 Notification of Positive and Negative Vials .................... 39
4.5.3 Removing Positive Vials................................................. 40
4.5.4 Removing Negative Vials ............................................... 42
4.6 Printing System Status Reports................................................. 43
4.7 Resolving Station Errors............................................................ 45
4.8 Identifying Anonymous Vials .................................................... 47
4.9 Power Failures and Manual Operation...................................... 49
5 - Reference
5.1 General..................................................................................... 51
5.2 Software Menu Tree ................................................................. 52
5.3 Display Types............................................................................ 52
5.3.1 Main Status Screen........................................................ 53
5.3.2 Main Activity Screen...................................................... 54
5.4 Icon Charts ............................................................................... 55
6 - Maintenance
6.1 General..................................................................................... 59
6.1.1 Instrument Symbols ...................................................... 60
6.2 Routine Maintenance ............................................................... 60
6.2.1 Air Filter Replacement ................................................... 60
6.2.2 Temperature Verification (QC)....................................... 61
6.2.3 Barcode Scanner Window ............................................. 62
6.2.4 Decontamination........................................................... 63
 u Preface

Contents

6.3.1.3 Rotor .............................................................. 68

6.3.1.4 RTD................................................................. 71
6.3.2 Front Panel Components............................................... 74
6.3.2.1 Front Panel Removal....................................... 74
6.3.2.2 Barcode Scanner Replacement........................ 76
6.3.2.3 Floppy Disk Drive Replacement....................... 77
6.3.2.4 Keypad/LCD Display Replacement ................... 79
6.3.3 Rear Cabinet Components.............................................81
6.3.3.1 Rear Cabinet Shell........................................... 81
6.3.3.2 Electrical Guard .............................................. 83
6.3.3.3 AC Power Distribution Board.......................... 84
6.3.3.4 Blower ............................................................ 85
6.3.3.5 Computer Board.............................................86
6.3.3.6 DC Power Distribution Board.......................... 87
6.3.3.7 Detector Board ............................................... 89
6.3.3.8 Fan ................................................................. 91
6.3.3.9 Heater ............................................................ 93
6.3.3.10 I/O Board ........................................................ 94
6.3.3.11 Main Transformer........................................... 96
6.3.3.12 On/Off Switch, Line Filter, and Interlock Switch 99
6.3.3.13 Power Supply (5V/+15V/–15V) Board........... 100
6.3.3.14 Power Supply (40V) Board............................ 101
7 - Troubleshooting
7.1 General................................................................................... 103
7.1.1 Instrument Service....................................................... 103
7.1.2 Instrument Symbols .................................................... 103
7.2 Error/Alert Messages............................................................... 104
 Appendix A - Limited Warranty ...................................................... 115
 Appendix B - Replacement Parts .................................................... 117
 Appendix C - Software Update Log ............................................... 119
 Appendix D - International Contacts ............................................. 121
 Appendix E - Supplemental Forms ................................................. 123
BACTEC™ 9050 System User’s Manual

Contents
1.1

Introduction

BACTEC 9050 Overview

The BACTEC 9050 instrument is designed for the rapid detection of bacte-
ria and fungi in clinical cultures of blood. Samples are drawn from patients
and injected directly into BACTEC culture vials. Vials are then entered into
the instrument as soon as possible to insure performance efficacy.
When microorganisms are present, they metabolize nutrients in the
culture medium, releasing carbon dioxide into the medium. A dye in
the sensor reacts with CO 2. This modulates the amount of light that is
absorbed by a fluorescent material in the sensor. The instrument’s
photo detectors measure the level of fluorescence, which corresponds
to the amount of CO  released by organisms. Then the measurement is
2

interpreted by the system according to preprogrammed positivity

parameters. (See Figure 1-1.)
At system start-up, the BACTEC 9050 instrument performs self-diag-
nostics and loads its operating instructions. Then the instrument begins
automated testing. A row of Light Emitting Diodes (LEDs) behind the
vials illuminate, activating the vials’ fluorescent sensors. The instru-
ment’s photo detectors then take the readings. A test cycle is com-
pleted every ten minutes. Positive cultures are immediately flagged by
BACTEC™ 9050 System User’s Manual

When positive vials are identified, the lab technologist pulls them from
the instrument for confirmation of results, and for isolation and identi-
fication of the organism.

Organism metabolic activity releases CO2...

Which reacts with dye in vial sensor.
LED, modulated by dye, activates fluorescent
material in sensor.
Photo detector reads fluorescence.
Raw data from detector is sent to computer…
Where positivity analysis is performed.
Positive vial indicator lights, audible alarm
sounds, positive stations are displayed.

 C 
 O
2 

Test
Results

LED
Photo
Detector

Computer

Raw Data
Positivity
Analysis

Figure 1-1. BACTEC Fluorescent Test Technology

 
Introduction

An instrument is capable of monitoring a total of 50 BACTEC culture

vials. The practical capacity is 5 culture sets per day with a 5-day test
protocol. The vials are arranged in 3 concentric rings designated A, B,
and C. The vials are continuously incubated at 35° C, and are agitated
for maximum recovery of organisms.
Major features of the BACTEC 9050 instrument include:
•Automated, continuous, unattended testing of cultures through
non-invasive fluorescent technology
•Minimum user interaction and handling
•Immediate notification of positives through an indicator lamp,
indication on the LCD display, and an audible alarm
•Simple user interface, with picture icons to guide you through
setup and routine operations
•Incubation and agitation for all cultures
•Proven BACTEC culture media

1.2 Instrument Overview

The BACTEC 9050 instrument (hardware) components are described in
the following paragraphs and are shown in Figure 1-2.  Controls and
indicators for these modules are discussed in Section 3 – Controls and
Indicators.
The major subsystems of the instrument include the following:
Rotor
The rotor contains 50 wells called “vial stations,” into which vials are
placed for incubation and testing. The rotor contains three rings of vial
stations designated A, B, and C. Each station is numbered uniquely
(1 - 50), but the system provides the ring letter designation (A, B, or C)
to help you quickly identify where to place or remove vials. One row of
stations (one station in each ring) contains the test LEDs that activate
vial sensors and the photo detectors which take the actual readings.
The rotor is mounted at a 20° angle and rotates to provide agitation to
culture vials. Agitation of cultures can improve both time to detection
and recovery of organisms. The rotor’s drive motor stops agitation
when the cabinet door is opened.
 
Introduction

Computer
The system computer stores all the system software, including the
software which controls instrument operations and the user interface,
which enables you to perform instrument operations, view vial
statuses, print reports, etc.
Floppy Disk Drive
A floppy disk drive is located on the front of the instrument to enable
the installation of software updates and to copy important data files to
floppy disk.
External Ports
Ports on the rear of the instrument allow the user to connect an
optional Remote Alarm unit and an optional printer. Two other ports
are for Field Service diagnostic use.
Instrument Calibration
Components of the BACTEC 9050 instrument are selected and designed
to maintain electrical and optical integrity throughout the product’s
life. All instruments are calibrated at the factory prior to shipment, and
should not require recalibration during the usable life of the
instrument, unless certain components are replaced. Calibration helps
to insure that any fluorescent series medium vial in any station will
have initial and final fluorescent values within a specified range.

1.3 Software Overview

The system software presents a simplified user interface on the LCD
Display, with picture icons to represent all the functions, operations,
setup parameters, and status conditions (see Section 5 – Reference for
charts of all icons). Routine system operations are performed by
pressing the teal soft key that corresponds to the definition shown on
the screen.
There are three basic types of displays:
Main Status Screen  – When the instrument door is closed, this
screen appears. It shows the number of vials that are positive,
negative, ongoing, available, and stations that are in error or
anonymous. Also shown are the current date and time and the
BACTEC™ 9050 System User’s Manual

Configuration Screens – Accessible from the Main Status Screen,

the Configuration Screens allow you to set the protocol length, the
time and time format, the date and date format, the audible alarm
volume, the instrument identification number, the DVE (Delayed
Vial Entry) threshold (feature not available for use in the USA), to
select the desired language for reports, to write data to a floppy
disk, and to update system software.
 Activity Screens – When the instrument door is opened, software
key definitions appear that enable you to enter new vials, remove
positive vials, remove negative vials, identify anonymous vials, and
resolve station error conditions.

Figure 1-3. Main Status Screen

1.3.1 Built-in-Test
The system software is designed to continuously monitor the electrical
and optical performance of all stations simultaneously. This
functionality, called BIT (for Built-in-Test), automatically monitors each
station every ten minutes for basic operational characteristics. These
tests continually verify that signal output for each station is within
design limits; this includes both empty stations and stations with
ongoing vials.
Two different signal levels are used to verify operation of the station
over the established signal range. Tests are performed on dark readings
(the output from the station when its excitation LEDs are off) and on
fluorescence unit readings (the output from the station when a vial is
present and the excitation LEDs are on).
Dark readings are evaluated to be below a maximum established range.
When they exceed that range, the software declares the station in error.
High dark readings may indicate a light leak in the cabinet or an
electrical failure within the instrument.
 
Introduction

reading occur, the software declares that station in error. This may
occur due to an electrical or optical component failure.
Additionally, the fluorescence readings are evaluated for consistency
while vials are in stations. If consecutive fluorescence unit readings vary
by more than a predetermined amount, the station is declared in error.
This feature determines the stability and the acceptability of a station
for use during protocol.
These protection features verify that calibration has been maintained
for all stations within the system, and insure that the user is alerted of
electronic or optical changes or failures which may be significant
enough to affect results.
The function of BIT can be demonstrated by entering a vial into the
instrument, and then removing that vial from the station without
scanning it out. The resulting error is the response of the BIT function.
The user may resolve the error by using the resolve errors soft key.

1.4 Manual Structure

This user’s manual contains the following sections:
Section 1 – Introduction  – provides an overview of the
BACTEC 9050 instrument and its uses in the microbiology
laboratory, its major hardware and software components. An
overview of this manual’s structure and conventions is also
included.
Section 2 – Installation and Setup  – gives specifications for
installing the BACTEC 9050 instrument and instrument setup.
Section 3 – Controls and Indicators – explains the use and
meaning of all controls and indicators of the system.
Section 4 – Operation  – provides instructions for routine daily
activities.
Section 5 – Reference – provides reference material on the user
interface.
Section 6 – Maintenance  – explains all system maintenance,
including parts replacements. Depending on the serial number
of your instrument, some parts replacements should be
BACTEC™ 9050 System User’s Manual

Test Protocol Duration (1/9)

There are three test protocol duration settings: General
media, Mycosis/IC–F* medium, and Myco/F Lytic medium.
When this display is first accessed, the test protocol for gen-
g en-
eral media (all media types other than Mycosis/IC–F* and
Myco/F Lytic) is highlighted. The default setting is 5 days. To
To increase or
decrease the number of days for general media, use the UP ARROW or
DOWN ARROW key. You can choose from 4 to 7 days.
* Not available for use in USA 
To adjust the test protocol duration for Mycosis/IC–F medium (not avail-
able for use in the USA) press the “move to other field” soft key to
highlight that field. Use the UP ARROW or DOWN ARROW key to
increase or decrease the displayed value. You can choose from 5 to 42
days. The default value is 14 days.
To adjust the test protocol duration for Myco/F Lytic medium, press the
“move to other field” soft key to highlight that field. Use the UP
ARROW or DOWN ARROW key to increase or decrease the displayed
value. You can choose from 5 to 42 days.The initial default value is 42
days. The recommended testing protocol for the following organisms
are: 7 days for yeast, 30 days for fungi, and 42 days for mycobacteria.
Press the “configuration” soft key to advance to the next setup display,
or press the “exit” soft key to exit configuration mode.
Time and Time Format (2/9)
When this display is first accessed, the minutes
value is highlighted. To adjust the minutes, use
the UP ARROW or DOWN ARROW key to increase
or decrease the displayed value.
To adjust the hours value, press the “move to other field” soft key to
highlight the hours field. Use the UP ARROW or DOWN ARROW key to
increase or decrease the displayed value.
To adjust the time format, press the “format” soft key (shown above).
The default time format (colon separated) is shown. Continue to press
the “format” soft key to rotate among the format choices until the
desired selection is shown. You can choose among period (.) or
comma ( , ) or colon
colon ( : ) separat
separators.
ors.
Press the “configuration” soft key to advance to the next setup display,
or press the “exit” soft key to exit configuration mode.
 
Installation and Setup

Date and Date Format (3/9)

When this display is first accessed, the year
value (at default, the right field) is high-
lighted. To adjust the year, use the UP ARROW
or DOWN ARROW key to increase or decrease
the displayed value.
To adjust the day value (at
( at default, the middle field), press
p ress the “move to
other field” soft key to highlight the day field. Use the UP ARROW or
DOWN ARROW key to increase or decrease the displayed value.
To adjust the month
mo nth value (at default, the left field), press the “move
“ move to
other field” soft key to highlight the month field. Use the UP ARROW or
DOWN ARROW key to increase or decrease the displayed value.
To adjust the date format, press the “format” soft key (shown above).
The default date format (MM/DD/YY) is shown. Continue to press the
“format” soft key to rotate among the format choices until the desired
selection is shown. You
You can choose from the following:
fo llowing:

Slash MM/DD/YY or DD/MM/YY or  YY/MM/DD or

separators ( / ) MM/DD/YYYY  DD/MM/YYYY   YYYY/MM/DD

Hyphen MM-DD-YY or DD-MM-YY or  YY-MM-DD or

separators ( - ) MM-DD-YYYY  DD-MM-YYYY   YYYY-MM-DD

Period MM.DD.YY or DD.MM.YY or  YY.MM.DD or

 YY.MM.DD
separators ( . ) MM.DD.YYYY  DD.MM.YYYY   YYYY.MM.DD
 YYYY.MM.DD

Press the “configuration” soft key to advance to the next setup display,
or press the “exit” soft key to exit configuration mode.
 Audible Alarm Volume (4/9)
Select the volume of the instrument’s audible alarm. The
default setting is 5, which is at the center of the volume
range. To increase or decrease the volume, use the UP
ARROW or DOWN ARROW key (a sample volume tone
sounds each time you adjust
a djust the setting). You
You can choose from 0 (audi-
ble alarm off) to 10 (loudest).
Press the “configuration” soft key to advance to the next setup display,
or press the “exit” soft key to exit configuration mode.
BACTEC™ 9050 System User’s Manual

Instrument Number (5/9)

Select the instrument identification number.
number. The default
d efault set-
ting is 1. To increase or decrease the instrument number, use
the UP ARROW or DOWN ARROW key. You can choose a
number from 1 to 99. If there is only one instrument at your
location, you should leave this value set at 1.
Press the “configuration” soft key to advance to the next setup display,
or press the “exit” soft key to exit configuration mode.
DVE Media Thresholds (6/9)
This feature is not available for use in the USA. For informa-
tion on use
use internationally,
internationally, refer to the BACTEC 9050
Delayed Vial Entry Instructions, MA–0113.

Language (7/9)
Select the language in which you want the System Status
Report to print. The default setting is English. To scroll
through the available selections, use the UP ARROW or
DOWN ARROW key. YouYou can choose from the following lan-
guage selections:
English
Spanish
French
German
Italian
Chinese
Japanese
Polish
Press the “configuration” soft key to return to the first setup display, or
press the “exit” soft key to exit configuration mode.
Save Data to Disk (8/9)
Under certain circumstances, Becton Dickinson will advise
you to save system data to a floppy disk. These circum-
stances include some error conditions and system malfunc-
tions. To
To use the function, insert a blank, formatted, write-
enabled floppy disk in the disk drive. Then press the “perform action”
 
Installation and Setup

Update Software (9/9)

From time to time, updated versions of the system soft-
ware may be provided to you. New software should be
installed as soon as it is received, and logged on the form
in Appendix 10 of this User’s Manual. Updated program
software is furnished on 3-1/2 inch floppy disk(s), labeled
“BACTEC 9050 System Software, Version y.yyz” (where “y.yy” is the
software version number, and “z” is the revision). The associated cata-
log number is also printed on the label.
To install a software update, insert the floppy disk containing the new
software into the disk drive, then press the “perform action” soft key to
initiate the update. The system reboots and then immediately begins to
update the system software (provided the disk is formatted, is not
write-protected*, and contains either the same or a later version of sys-
tem software). The names of any files being updated appear on the
LCD Display, as well as a progress indication. When the update is com-
plete, the user interface loads and you may proceed with normal sys-
tem operation.
* Note that write-protection does not have to be disabled with
version 1.1 or later of system software

2.5 System Start-up

Whenever power is applied to the instrument, the system is initialized,
performs self-diagnostics, and reports any problems to the error file. If
any files are missing or corrupted which would prevent proper opera-
tion of the system, the start-up process is aborted. If not, the computer
loads the operating instructions and begins culture testing. <
BACTEC™ 9050 System User’s Manual
 
Controls and Indicators

3.2 On/Off Switch

The system power (On/Off) rocker switch is on the rear of the instru-
ment at the bottom right (see Figure 3-2).
3-2). When in the “O” (Off) posi-
tion, power is removed from the instrument. When in the “|” (On)
position, power is applied to the instrument. Power must be turned on
for the incubator, agitator, and culture testing modules to work. For
normal operation, the power should remain On at all times (except dur-
ing some maintenance procedures).

On/Off Switch

Figure
Figure 3-2.
3-2. On/O
On/Off
ff Switch
Switch

3. 3 Keypad and LCD Display

The keypad and LCD display are located on the front of the
BACTEC 9050 instrument, at the bottom
bottom center.
center. The keypad is used to
enter information and issue commands to the instrument. The LCD dis-
play presents setup and status information. See Figure 3-3.
3-3.
The controls and indicators of the keypad and LCD display are pre-
sented in clockwise order from the top right of the module.
BACTEC™ 9050 System User’s Manual

UP ARROW Soft Key Display Area SILENCE

(Increase  Assignment
 Assign mentss  ALARM
Key) Key

DOWN
 ARROW
(Decrease Key) HOME
ROTOR
Key
Soft Keys

New Positives Indicator  Alarm Ind icator

Brightness Dial

Figure 3-3.
3-3. Keyp
Keypad
ad and LCD Display
Display

3. 3. 1 SILENCE AL
ALARM Ke
Key
The SILENCE ALARM key is located at the top right of the Keypad/LCD
Display module. When pressed, it turns off the audible alarm for the
current “alert” alarm. It does not silence “door open” type audible
alarms. (See Section 3.7 –
3.7 – Audible Tones and Alarms,
Alarms, for an explanation
of alarm types and tones.)

3.3.2 HOME ROTOR Key

The HOME ROTOR key is located on the right side of the Keypad/LCD
Display module, below the SILENCE ALARM key. When pressed, it
causes the rotor in the instrument to return to the “home” position,
with the temperature standard bottles in the 12:00 position. The HOME
ROTOR key is active only when the instrument door is closed and the
rotor is in motion.
It is not necessary to home the rotor prior to opening the door. How-
ever, you may find it easier to locate vial stations when the rotor is
homed, since the rotor’s position then corresponds to the representa-
tion in the display.
 
Controls and Indicators

3. 3. 3 Alarm Indicator
The Alarm Indicator is located on the right side of the Keypad/LCD Dis-
play, at the bottom. This light flashes yellow whenever the system
encounters an error condition that requires operator attention. The
indicator continues to flash until the condition is corrected.
When an alarm occurs, an icon (resembling the one above the Alarm
Indicator) appears in the soft key assignments area of the Main Status
Screen. Press the soft key corresponding to the System Alert icon. The
first error code is shown
sho wn on the display. Correct any error conditions as
soon as possible by following the directions on the door placard and in
Section 7 – Troubleshooting.
If there is an audible alarm sounding, you can silence it by pressing the
SILENCE ALARM Key.

3.3.4 Brightness Dial

The Brightness Dial is located
lo cated below the Keypad/LCD Display,
Display, at the cen-
ter. It is a knurled rotary dial. Rotate the dial clockwise to increase the
overall brightness of the LCD Display screen. Rotate counterclockwise
to reduce the brightness. It is not uncommon to have to change the
brightness setting if you change your viewing angle.

3.3.5 Soft Keys

The six software (soft) keys are located near the bottom
bot tom of the Keypad/ 
LCD Display, at the center. These keys are teal colored. None of the keys
has a fixed function – the functions of the keys vary depending on the
current active display. Each display shows icons representing the cur-
rent soft key assignments at the bottom of the screen. To perform the
function represented by the icon, press
pre ss the corresponding soft
sof t key.
key.
A complete icon legend is provided in Section 5 – Reference.
Reference.

3. 3. 6 New Posi
siti
tiv
ve Indicator
The New Positive Indicator is located on the left
l eft side of the Keypad/LCD
Display, at the bottom. This light flashes red whenever a new positive
culture is detected. The indicator continues flashing until all positive
vials are removed from the instrument. Refer to Section 4.5 for
4.5 for infor-
mation on removing positive vials.
BACTEC™ 9050 System User’s Manual

3.3.7 DOWN/UP ARROW Keys

The DOWN and UP ARROW keys are located on the left side of the Key-
pad/LCD Display. The DOWN ARROW key, represented by a down
arrowhead, is used to decrease a displayed value, or to scroll down-
ward in a list. The UP ARROW key, represented by an up arrowhead, is
used to increase a displayed value, or to scroll upward in a list.

3.3.8 Display Area

The Display area is located at the center of the Keypad/LCD Display. It is
used to present information to you, and to show the soft key defini-
tions that allow you to perform routine operations.
The LCD Display is programmed to automatically dim after 5 minutes of
inactivity. To return the brightness to normal, press one of the unas-
signed keys (such as the rightmost soft key).
More information on displays is presented in Section 5 – Reference.

3.4 Door Interlock Switch

When the door of the instrument is opened, a door switch senses the
condition (see Figure 3-1). This switch tells the system to immediately
stop the rotor drive motor, start the door open timer, and discard the
results of any tests in progress.

WARNINGS
THE DOOR SENSOR HELPS PROTECT YOU
FROM POSSIBLE INJURY FROM THE
MOVEMENT OF THE ROTOR. DO NOT
TAMPER WITH THE DOOR SENSOR IN ANY
WAY, OR ATTEMPT TO DEFEAT ITS
FUNCTION.
NEVER ATTEMPT TO INSERT OR REMOVE
VIALS WHEN THE ROTOR IS IN MOTION.
 
Controls and Indicators

3.5 Floppy Disk Drive

The floppy disk drive is located on the front of the instrument, at bottom
left. Its primary purposes are to enable you to save data to floppy disk,
and to perform software updates when they are released. See Figure 3-4.

3.5.1 Floppy Disk Indicator

The floppy disk drive indicator light is on the left side of the drive below
the insertion slot. When off it indicates that no activity is occurring in
the drive. When on or flashing, it indicates that the disk drive is access-
ing a floppy disk. Do not attempt to eject a floppy disk while this
indicator is lit.

3.5.2 Floppy Disk Eject Button

The floppy disk drive eject button is located to the lower right of the
insertion slot. When a floppy disk is inserted fully into the slot, this but-
ton extends itself. To remove a disk, fully depress the eject button. Do
not attempt to eject a floppy disk while the floppy disk indicator is
lit.

Floppy Disk Indicator

Floppy Disk Eject Button

Figure 3-4. Floppy Disk Drive Controls and Indicators

3.6 Barcode Scanner

The barcode scanner is located to the right of the Keypad/LCD Display
BACTEC™ 9050 System User’s Manual

To scan a vial barcode, place the vial in the alignment block in front of the
scanner. If necessary, rotate the vial slightly until the acknowledgment
beep sounds (indicating that the barcode was scanned successfully).

3.7 Audible Tones and Alarms

Eight different types of sounds are generated by the BACTEC 9050
instrument as you perform operations. Each of the sounds is unique.
These tones are designed to keep you informed about various opera-
tional states of the instrument.

Type Example Sound

Informational

Acknowledge Scanning a barcode Single short high beep

In Configuration mode,
Sample Three tones progressing
the audible alarm
audible alarm from high to low (“Figaro”)
volume was adjusted

Activity
All positives are removed Three short fast high beeps
complete

Door closed Door was closed Two short fast high beeps

Rotor homed after Five tones progressing

Rotor home HOME ROTOR key was from low to high
pressed (arpeggio)

 Alarm

Short high beep then short

Unexpected barcode
Activity error low beep - sequence
scanned
repeated four times

Single medium beep - one

Alert New positive vial second on, three seconds
off, repeating
 
Operation

4.5 Positive and Negative

Specimens
4.5.1 General
Many positive cultures will be detected in the first 24 hours after inocu-
lation, but ongoing negative vials must still be kept for several days to
assure maximum recovery. With the BACTEC 9050 instrument, vials are
typically held for 5 – 7 days before they are discarded as negative. Each
laboratory should set the protocol length based on its own policies and
conditions. Your protocol length can be from 4 – 7 days.
A subculture and a Gram stain should be performed from each positive
vial. In most cases, organisms will be seen and a preliminary report can
be made to the physician. Preliminary antimicrobial susceptibility and
identification procedures may also be set up from fluid in the culture
vials. The results from these preliminary tests should be confirmed by
using standardized laboratory procedures.
The system allows re-entry of instrument-positive vials for up to 3 hours
after their removal (subject to the conditions described in Section
4.5.3). The re-entry feature resets positivity routines, retains previous
test readings, and continues to test the vial as an ongoing culture.
For a maximum yield of isolates, negative cultures may be stained and/ 
or subcultured during the test period or immediately prior to their dis-
posal.

4.5.2 Notification of Positive and Negative

Vials
The system notifies you of new positive cultures in several ways:
• New Positive indicator lamp on the front of the instrument
flashes.
• On the Main Status display, the station is displayed as a filled
circle with a plus sign (+) in it, and the positives total in the
Summary reflects the number of positives in the instrument.
• When the door is opened, the “remove positives” soft key
appears in the soft key assignment area of the LCD Display.
til sile ed, th udible al unds (if enabled)
BACTEC™ 9050 System User’s Manual

Negative cultures may exist as ongoing negatives (in protocol) and out-
of-protocol negatives. You are notified of these conditions as follows:
• Ongoing Negatives – On the Main Status display, the station is
displayed as a filled circle.
• Out-of-Protocol Negatives – On the Main Status display, the
station is displayed as a filled circle with a minus sign (–) in it.
Also, the negatives count in the Summary reflects the number
of final negatives in the instrument. No audible alarm sounds
for notification. When the door is opened, the “remove
negatives” soft key appears in the soft key assignment area of
the LCD Display.

4.5.3 Removing Positive Vials

Press the SILENCE ALARM key to silence the audible alarm. Open the
instrument door. Follow the procedure shown in Figure 4-2.

Press “remove positives” soft key

If vial was originally entered manually

If vial was originally entered by scanning (by selecting media type), the following
barcode, the following display appears: display appears:

Remove vial from specified station and Remove vial from specified station and
scan vial barcode, or leave vial in and press “force station available” soft key,
press DOWN ARROW to skip to next or leave vial in and press DOWN ARROW
positive station. to skip to next pos itive station.

or or

Repeat until all positives are removed. Repeat until all positives are removed.
Then press “exit” soft ke y. Then press “exit” soft ke y.
 
Operation

NOTE

The New Positive indicator remains lit until all new positive
vials are removed through the Remove Positives operation.

Instrument positive vials can be returned to the instrument for

additional testing subject to the conditions described below.

Returning Positive Vials to the Instrument for Further Testing

The system allows you to return a pulled positive vial – if the vial was
originally scanned into the instrument – for up to 3 hours after removal
(or until the vial goes out of protocol, whichever is earlier). The re-entry
feature resets positivity routines, retains previous test readings, and
continues to test the vial as an ongoing culture. If the vial is not
returned within the 3-hour re-entry window, previous test results are
discarded. If the vial was manually entered (by selecting media type),
the system treats it as a new vial if it is removed and subsequently re-
entered.
The following precautions should be observed when using the vial re-
entry feature. Instrument-positive vials should be subcultured and
Gram stained upon removal from the instrument. Even though a 3-
hour re-entry window exists for the return of a vial to an ongoing sta-
tus, the vial should be returned to the instrument as soon as possible.
Vials should remain at room temperature while they are out of the
instrument.
To return a pulled positive vial to the instrument for further testing,
open the door. Press the “vial entry” soft key. Next scan the vial’s bar-
code label. Place the vial in the indicated station (this may differ from
the original station). Previous test results are retained only in the fol-
lowing circumstances:
• You originally scanned the vial barcode when entering it in the
instrument.
• You return the vial within the required time frame.
• You scan the vial label to re-enter the vial.
• You return the vial to the same instrument from which it was
removed.
BACTEC™ 9050 System User’s Manual

 Rotor Status Icons

Icon Meaning Icon Meaning

Rotor is in intermediate
Temperature standards
position

Rotor is at home position Rotor is rotating

 Alert/Error Notification Icons

Icon Meaning Icon Meaning

Activity error or
System alert
instrument full

Problem with disk

Power was removed at
inserted or no disk is
this time
inserted

Power was restored at

this time

 Activity Icons

Icon Meaning Icon Meaning

Set individual vial

Vial entry
protocol

Remove positives Remove negatives

Resolve station errors Identify anonymous

 
Reference

 Media Type Icons (for manual vial entry)

Icon Meaning

BACTEC Plus Aerobic/F medium (25 ml)

BACTEC Plus Anaerobic/F medium (25 ml)

BACTEC Plus Aerobic/F medium (40 ml)

BACTEC Plus Anaerobic/F medium (40 ml)

PEDS Plus/F medium

Lytic/10 Anaerobic/F medium

Mycosis IC/F medium (not available for use in
USA)
Myco/F Lytic medium

BACTEC Standard/10 Aerobic/F medium

BACTEC Standard Anaerobic/F medium

Miscellaneous Actions Icons

Icon Meaning Icon Meaning

Confirm your selection/  Cancel your selection/ 

operation operation

Scroll down or decrease

Scroll up or increase value
value

Perform the desired

Move to other field (left)
action

Move to other field Value can be adjusted up/ 

(right) down
BACTEC™ 9050 System User’s Manual

Configuration Icons

Icon Meaning Icon Meaning

Set Protocol
Set time or date format
duration

Set date Set time

Set speaker volume Set instrument number

Set report language English language

Spanish language Chinese language

Italian language German language

Japanese language French language

Polish language Set DVE threshold

Aerobic threshold Anaerobic threshold

Copy data to disk Update software

Writing data to disk <

6.1 General

Maintenance

The BACTEC 9050 instrument requires minimal maintenance from the

user to provide reliable performance. Daily verifications include: check-
ing the incubator temperature and printer paper supply (if configured).
Routine preventive maintenance consists only of a monthly changing or
cleaning of the cabinet air filters. All other procedures are on an “as
needed” basis. Any maintenance or repair not described in this section
should be performed by BD personnel only.

WARNING
 ALL MAINTENANCE AND REPAIR OTHER THAN
THE PROCEDURES DESCRIBED IN SECTION 6 –
MAINTENANCE, MUST BE PERFORMED BY
QUALIFIED SERVICE PERSONNEL. FOR ALL
INSTRUMENTS WITH SERIAL NUMBERS FROM
1000 – 1695, PARTS REPLACEMENTS SHOULD BE
PERFORMED BY SERVICE PERSONNEL ONLY.
BACTEC™ 9050 System User’s Manual

(Rear of Instrument
with Cover Removed)

Driver Motor
(Second Person
Pushes Down)

Figure 6-6.
6-6. Rotor Removal
Removal – Pushing
Pushing Down Driver Motor
Motor
 
Maintenance

6.3.1.4 RTD
The RTD (resistance temperature device, or temperature sensor) is
located behind the rotor on the right side (facing
( facing the front). To
To remove
the RTD, follow the steps below and refer to Figures 6-7, 6-8,
6-8, and 6-9.
Required Materials
Small Phillips Screwdriver
To Remove the RTD
1 Remove the rotor (Section 6.3.1.3)
6.3.1.3) and rear cabinet shell
(Section 6.3.3.1).
6.3.3.1).
2 Unscrew the small phillips screw on the black mounting clip.
3 Push the rubber grommet in the through-hole through to the
front of the instrument.
4 Unplug the maroon 3-wire connector from the I/O Board
(located near the front end of the I/O Board).
5 Feed the RTD cable through the through-hole to the front of
the instrument and remove the RTD.
6 Replace the RTD by reversing the above steps. Be sure to
replace the rubber grommet in the through-hole.
BACTEC™ 9050 System User’s Manual

Phillips Screw

Grommet

Figure 6-7. RTD Remov

Removal
al (A)
 
Maintenance

I/O Board (toward rear )

RTD Cable

Figure 6-8. RTD Removal (B)

(Push foam
out of way)

Feed cable through

through-hole
BACTEC™ 9050 System User’s Manual

6.3.2 Front Panel Components

6.3.2.1 Front Panel Removal
The front panel, which is located at the bottom of the instrument, must
be removed to replace the Barcode Scanner, Floppy Disk Drive, or Key-
pad/LCD Display. Refer to Figures 6-10 and 6-11.
Required Materials
Medium Phillips Screwdriver
Small Flathead Screwdriver
To Remove the Front Panel
1 Unscrew the two phillips screws under the Keypad/LCD
Display.
2 Disconnect the wide flat ribbon cable by pulling outward on
the tabs at the connector’s end.
3 Disconnect the d-shaped connector (use the small flathead
screwdriver to loosen the screw locks).
4 Disconnect the barcode scanner and remove the panel
assembly.
5 Replace the front panel by reversing the above steps. Be sure
to reattach all cables when replacing the front panel.

(Front of Instrument)

Phillips Screws
 
Maintenance

(Front of
Instrument)

Unplug 2 Cables

Figure 6-11. Front Panel Removal (B)

BACTEC™ 9050 System User’s Manual

6.3.2.2 Barcode Scanner Replacement

To replace the barcode scanner, follow the steps below. Refer to
Figure 6-12.
Required Materials
Phillips Screwdriver (some instruments require Flathead Screwdriver
instead)
To Replace the Barcode Scanner
1 Remove the front panel (Section 6.3.2.1).
2 Remove the four phillips screws. (Some instruments have
captive fasteners that are designed to be loosened by hand or
with a flathead screwdriver.)
3 Remove the whole scanner/bracket assembly.
4 Replace the assembly by reversing the above steps.

(Front of Instrument
[Front Panel Removed])

Remove 4 Screws

Figure 6-12. Barcode Scanner Removal

BACTEC™ 9050 System User’s Manual

6.3.3.13 Power Supply (5V/+15V/–15V) Board

The 5V/±15V Power Supply is located at the top of the right electronics
compartment, toward the front of the instrument. (The 40V Power
Supply is right next to this board, toward the rear of the instrument.)
To replace the 5V/±15V Power Supply, follow the steps below and refer
to Figure 6-31.
Required Materials
5/16” Hex Nut Driver
To Replace the Power Supply Board
1 Remove the rear cabinet shell as described in Section 6.3.3.1.
2 Remove the Electrical Guard as described in Section 6.3.3.2.
3 Unplug the white connector on the top of the board.
4 Unplug the connector on the bottom of the board.
5 There is one nut on each of the board’s four corners. Remove
these nuts and remove the board.
6 Replace the board by reversing the above steps.

40 V Power 5V/+15V/-15V
Unplug Supply Board Power Supply Board
Connector

Unplug
Connector

Remove 4 Remove
Nuts 4 Nuts

Unplug Unplug
Connector
 
Maintenance

6.3.3.14 Power Supply (40V) Board

The 40V Power Supply is located at the top of the right electronics
compartment, toward the rear of the instrument. (The 5V/±15V Power
Supply is right next to this board, toward the front of the instrument.)
To replace the 40V Power Supply, follow the steps below and refer to
Figure 6-32.
Required Materials
5/16” Hex Nut Driver
To Replace the Power Supply Board
1 Remove the rear cabinet shell as described in Section 6.3.3.1.
2 Remove the Electrical Guard as described in Section 6.3.3.2.
3 Unplug the white connector on the top of the board.
4 Unplug the connector on the bottom of the board.
5 There is one nut on each of the board’s four corners. Remove
these nuts and remove the board.
6 Replace the board by reversing the above steps.

40 V Power 5V/+15V/-15V
Unplug Supply Board Power Supply Board
Connector

Unplug
Connector

Remove 4
Remove
Nuts
4 Nuts

Unplug Unplug
Connector
BACTEC™ 9050 System User’s Manual
7.1
7.1.1
General

Troubleshooting

Instrument Service
If your BACTEC 9050 instrument malfunctions or operates unusually in
any way, you may initially attempt to solve the problem by following the
procedures in this section. All other servicing attempts will terminate the
responsibility of the manufacturer under the terms of the warranty.
If you cannot repair a system malfunction, contact your local BD
representative (contact numbers are listed in Appendix D).
This section discusses error messages, which appear when the system has
encountered a known problem. These messages are listed in numerical
order, along with possible causes of the message and corrective actions.

7.1.2 Instrument Symbols

   
From Left – Symbol for electrical hazard, Symbol for 
BACTEC™ 9050 System User’s Manual

7.2 Error/Alert Messages

CAUTION
When the system notifies you of alerts and
errors, you should immediately respond to
the condition.

When the system encounters an alert or error condition, the error code
is either displayed on the screen or written into the System Alert list.
The error code is an abbreviation for the conditions described in the
listing below.
Codes in the E30 series (E30, E31, etc.) are not written into the System
Alert list, but are displayed on the screen when they occur. (They also
cause the Activity Error tone to sound [sequence of short high beep
and short low beep repeated four times].) These are activity (or
“workflow”) types of errors. In most cases, this means that some action
you have performed was not what the system expected, but you can
usually perform the correct action, as recommended below, without
exiting the current operation. These activity errors are flagged by the
Activity Error icon:

System alerts, which comprise all error codes except those in the E30
series, are reported in the System Alert list. These errors cause the Alert
tone (medium beep on for one second, off for 3 seconds, repeating) to
sound (if it is enabled). Also the System Alert icon appears on the Main
Status Screen. The errors must be reviewed to clear the system alert
condition. The System Alert list can be viewed from the Main Status
Screen by pressing the “system alert” soft key:

The error messages are listed in numerical order. Error sub-codes are
 
Troubleshooting

CAUTION
If any error sub-codes other than those listed
here appear, note the sub-code and contact
BD for assistance.
If the recommended corrective actions do not
solve the problem, contact BD.

E01 Temperature Alarm

POSSIBLE CAUSE(S)
Incubator temperature is too high (00000001) or too low (00000002).
• Door was kept open too long.
• Room temperature is not within recommended range.
• Air filters are dirty, restricting fresh air intake.
• RTD, heater, fan, or blower is defective.
CORRECTIVE ACTION(S)
 Check current incubator temperature on LCD Display to see if
temperature is still too high or too low.
 Minimize number and duration of door openings.
 Make sure room temperature is within range specified in
Section 2 – Installation and Setup.
 Clean or replace air filters.
 Check thermometer vial to see if manual reading agrees with
displayed reading.
 Reset manual thermostat (see Figure 6-26 for location of Reset
button).
 00000001 – At temperatures in excess of 38° C, viability of
many organisms may have been lost. Recollection of speci-
mens should be considered.
BACTEC™ 9050 System User’s Manual

E02 Rotor RPM out of spec

POSSIBLE CAUSE(S)
Rotor speed is too fast, too slow (00000002), or stopped (00000004).
• Something is impeding or jamming movement of rotor.
• A vial is not seated in the station.
CORRECTIVE ACTION(S)
 Check for and remove anything that may be impeding or
 jamming the movement of the rotor.
 Make sure all vials are seated in stations.
 All vials should be subcultured.

E05 Temperature standardization error

The temperature standards are out of tolerance.

POSSIBLE CAUSE(S)
• Temperature standards are not seated.
• Cover over temperature standards is not in place.
• Debris, spilled media, or blood inside instrument is affecting
temperature standard readings
CORRECTIVE ACTION(S)
 Check/reseat temperature standards.
 Check/adjust cover over temperature standards.
 Check that there is no debris, spilled media, or blood inside
instrument. Clean if necessary.

E06 Rotor configuration error

POSSIBLE CAUSE(S)
• Rotor is not mounted correctly.
Hold-d t (hubc )i ot ti ht
BACTEC™ 9050 System User’s Manual

Item Catalog Number

Floppy disks 445847

Floppy Disk Drive 445813

Heater 445808

I/O Board 445818

Knob 445803

Main Transformer 445824

Manual, BACTEC 9050 System User’s (ea.) 445845

On/Off Switch/Circuit Breaker 445822

Placard, Instrument (ea.) 445846

Power Control/AC Distribution Board 445823

Power Supply +5V, +/– 15V 445825

Power Supply +40V 445826

Rotor 445802

RTD Assembly 445809

Temperature Standard 445652

Thermometer, Temperature QC (ea.) 445844 <

 Date
Received
h
Software Update Log

Software
Version
Date
Installed
Installed
By
Notes
BACTEC™ 9050 System User’s Manual
7 Loveton Circle
i
International Contacts

BD

Sparks, Maryland 21152 USA 

Voice: (410) 316-4000 •Fax: (410) 527-0244
Toll-Free: Technical Services: 1-800-638-8663
Field Service: 1-800-544-7434
Customer Service: 1-800-638-8663
www.bd.com/microbiology
2771 Bristol Circle
Oakville, Ontario
Canada L6H 6R5
Voice: (905) 855-5550, 800-268-5430
Monte Pelvoux 111 • 9th Floor
Col. Lomas de Chapultepec
11000 Mexico D.F.
Voice: 52 5 237 1200 • Fax: 52 5 237 1287
11 Rue Aristide Bergès BP4
38800 Le Pont de Claix France
Voice: 33 476 68 36 36 • Fax: 33 476 68 34 95
 Index 

E33, 112, 113

E34, 113
E50, 113

F
floppy disk drive
eject button, 31
indicator, 31
overview, 5
replacement, 77

H
HOME ROTOR key, 28
how to
collect specimens, 34
enter new vials, 35
identify anonymous vials, 47
perform media Quality Control, 34
prepare specimens, 34
print a System Status Report, 44
re-enter positive vials for further testing, 41
remove negative vials , 42
remove positive vials, 40
resolve station errors, 45
scan a vial barcode, 32, 37

I
icons, 53–58
installation
requirements, 15, 18
software, 23
instrument
alarms and errors, 45
barcode scanner, 4
calibration, 5, 7
computer, 5
configuration, 19
 See also instrument: setup
daily checks , 35
decontamination, 63
dimensions, 16
BACTEC™ 9050 System User’s Manual

floppy disk drive, 5

general requirements, 15
keypad, 4
layout, 26
LCD display, 4
lifting, 18
overview, 1–5
power failures, 49
rings, 3
rotor, 3
routine maintenance, 60
service, 103
setup, 19–22
specifications, 16
stations, 3
symbols, 10
temperature, 3, 35, 60
warnings and cautions, 10

K 
keypad
key designations, 9
key functions, 28
overview, 4
replacement, 79
soft keys, 29

L
LCD display
automatic dimming, 30
brightness dial, 29
information presented, 53
overview, 4
replacement, 79
types of displays, 53–55

M
maintenance
air filter replacement, 60
barcode scanner window, 62
decontamination, 63
 Index 

related documentation, 8
text box types , 10
warnings and cautions, 10

N
negative vials
general, 39
notification, 39
removing, 42
new positive indicator, 29, 41

O
On/Off switch, 27

P
parts replacements
AC power distribution board, 84
barcode scanner, 76
blower, 85
computer board, 86
damper cylinder, 65
DC power distribution board, 87
detector board, 89
door sensor switch, 67
electrical guard, 83
fan, 91
floppy disk drive, 77
front panel, 74
heater, 93
I/O board, 94
keypad/LCD display, 79
main transformer, 96
On/Off switch, 99
part catalog numbers, 117
power supply (40V) board, 101
power supply (5V/ +15V/–15V ) board, 100
rear cabinet shell, 81
rotor, 68
RTD, 71–73
ports, 5
positive vials
BACTEC™ 9050 System User’s Manual

power switch, 27

R
rings, 3
rotor
and door sensor switch, 30
icon, 53
overview, 3
removal, 68–70
vial loading to balance, 37

S
screen
 See LCD display
setup
audible alarm volume, 21
date, 21
date format, 21
DVE media thresholds, 22
instrument number, 22
language, 22
test protocol duration, 20
time, 20
time format, 20
SILENCE A LARM key, 28
soft keys, 29
software
display types, 53–55
icons, 53–58
menu tree, 52
overview, 7
save data to disk , 22
setup, 19–22
types of displays, 55
update log, 119
updates, 23
specimen
collection, 34
preparation, 34
UNIVERSAL PRECAUTIONS , 34
stations, 3
 Index 

format, 20
setting, 20
tones, 32

V
vials. See culture vials

W
warnings, 11
warranty, 115