St Paul University Philippines
Tuguegarao City 3500
Tel: 078-846-4444; 078-396-1987 to 1994
Fax: 078-846-4305
www.spup.edu.ph
SCHOOL OF NURSING AND ALLIED HEALTH SCIENCES
College of Medical Technology
MTE113: BIOCHEMISTRY FOR MLS
Laboratory Activity #4
Study Questions for Lipids
Name: __________________________ Date Submitted: ______________
Yr., Section & Set: ____________ Student Number: ______________
Briefly answer the following:
I. TESTS FOR LIPIDS:
A. Spotting Effect
1. What is the purpose of this test?
2. What property of lipids is detected by this test?
3. Are the results of this test conclusive? Explain your answer.
Laboratory Activity #04│Page 1 of 9
B. Solubility Test
1. With the aid of the structural formula, explain the demonstrated
solubility/insolubility of vegetable oil and lecithin to:
1.1 Water
1.2 Methylene Chloride
1.3 Ether
1.4 Toluene
C. Test for Unsaturation (Bromine Water Test)
1. Show the sample equation involved in the reaction between bromine
water and your sample/s which gave a positive result:
Laboratory Activity #04│Page 2 of 9
2. Provide a possible explanation for the difference in results
between the vegetable oil and the lecithin.
D. Acrolein Test
1. Show the sample equation involved in the reaction between:
1.1 Glycerol and potassium bisulfate
1.2 Cooking oil and potassium bisulfate
1.3 Lecithin and potassium bisulfate
Laboratory Activity #04│Page 3 of 9
1.4 Toluene
II. DETECTION OF BRAIN LIPIDS
A. Molisch Test
1. Show the sample equation involved in the reaction between Molisch
reagent and your sample/s which gave a positive result:
2. What is the compound responsible for your observation?
3. What group of lipids was identified by this test?
Laboratory Activity #04│Page 4 of 9
B. Ninhydrin Test
1. Show the sample equation involved in the reaction between Ninhydrin
reagent and your sample/s which gave a positive result:
2. What is the compound responsible for your observation?
3. What group of lipids was identified by this test?
C. Soda Lime Test
1. Show the sample equation involved in the reaction between Soda
Lime reagent and your sample/s which gave a positive result:
Laboratory Activity #04│Page 5 of 9
2. What is the compound responsible for your observation?
3. What group of lipids was identified by this test?
D. Ammonium Molybdate
1. Show the sample equation involved in the reaction between Ammonium
Molybdate reagent and your sample/s which gave a positive result:
2. What is the compound responsible for your observation?
3. What group of lipids was identified by this test?
Laboratory Activity #04│Page 6 of 9
E. Leibermann-Burchard Test
1. Show the sample equation involved in the reaction between the
reagent and your sample/s which gave a positive result:
2. What is the compound responsible for your observation?
3. What group of lipids was identified by this test?
4. Why must one take the precaution of using only very dry containers
and pieces of equipment?
Laboratory Activity #04│Page 7 of 9
III. Complete the following table:
A. Test for Lipids, their Principle and Purpose:
TEST PRINCIPLE PURPOSE
1. BROMINE
WATER
2. ACROLEIN
3. MOLISCH
4. NINHYDRIN
5. SODA LIME
6. AMMONIUM
MOLYBDATE
7. LEIBERMANN-
BURCHARD
B. Test for Lipids, their Reagent Composition and Function:
FUNCTION OF EACH
TEST REAGENTS USED
COMPONENT
1. MOLISCH
Laboratory Activity #04│Page 8 of 9
FUNCTION OF EACH
TEST REAGENTS USED
COMPONENT
2. NINHYDRIN
3. SODA LIME
4. AMMONIUM
MOLYBDATE
5. LEIBERMAN
BURCHARD
-------------------------------------------------------------------
Nothing Follows!!!
Deadline of submission: _____________________ REFERENCES:
__________________________________
Prepared by:
__________________________________
__________________________________
NESTDIVIE U. PASCUAL, RMT __________________________________
Instructor __________________________________
__________________________________
__________________________________
__________________________________
KAREN TANGUILAN, RMT __________________________________
Instructor
__________________________________
__________________________________
__________________________________
_
Laboratory Activity #04│Page 9 of 9
