ISO 374-5:2016(en)

Protective gloves against dangerous chemicals and micro-organisms — Part 5:

Foreword

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ISO 374-5 was prepared by the European Committee for Standardization (CEN) in collaboration with ISO
Technical Committee ISO/TC 94, Personal safety — Protective clothing and equipment, Subcommittee
SC 13 Protective clothing in accordance with the Agreement on technical cooperation between ISO and
CEN (Vienna Agreement).
ISO 374 consists of the following parts, under the general title Protective gloves against dangerous
chemicals and micro-organisms:

 — Part 1: Terminology and performance requirements for chemical risks

1   Scope

This part of ISO 374 specifies the requirements and test methods for protective gloves intended to protect
the user against micro-organisms.

NOTE If other protection features is to be needed, e.g. chemical risks, mechanical risks, thermal risks,
electrostatic dissipation etc., the appropriate specific performance standard is to be used in addition.
Further information on protective gloves standards can be found in the EN 420.

2   Normative references

The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
ISO 374-5:2016(en)
Protective gloves against dangerous chemicals and micro-organisms — Part 5:
Terminology and performance requirements for micro-organisms risks
 EN 374-2:2014, Protective gloves against dangerous chemicals and micro-organisms — Part 2:
Determination of resistance to penetration
 EN 420:2009, Protective gloves  — General requirements and test methods
 ISO 16604:2004, Clothing for protection against contact with blood and body fluids  —
Determination of resistance of protective clothing materials to penetration by blood-borne
pathogens  — Test method using Phi-X 174 bacteriophage

3   Terms and definitions

For the purposes of this document, the following terms and definitions apply.
3.1
protective gloves against micro-organisms
protective gloves which form a protective barrier to microbiological agents
Note 1 to entry: Microbiological agents are bacteria or virus or fungi.
3.2
bacteria
very large group of micro-organisms comprising one of the three domains of living organisms, they are
prokaryotic, unicellular, and either free-living in soil or water or parasites of plants or animals
3.3
virus
any of various simple sub-microscopic parasites of plants, animals, and bacteria that often cause disease
and that consist essentially of a core of RNA or DNA surrounded by a protein coat
Note 1 to entry: Unable to replicate without a host cell, viruses are typically not considered living
organisms.
3.4
fungi
any of numerous eukaryotic organisms of the kingdom Fungi, which lack chlorophyll and vascular tissue
and range in form from a single cell to a body mass of branched filamentous hyphae that often produce
specialized fruiting bodies
Note 1 to entry: The kingdom includes the yeasts, moulds and smuts.

4   Sampling

4.1   Sampling for viral penetration testing

The test specimen shall be taken from the palm area. If the glove is longer than or equal to 400  mm and if
the cuff is claimed to protect against micro-organism risks, additional test specimens shall be taken where
the centre is 80 mm from the end of the cuff (see Figure 1). For further instructions, see ISO 16604:2004,
Clause 7.
In the case of seams in the hand area, this area shall be tested.
Figure 1 — Additional sample location for gloves longer than 400 mm
Dimensions in millimetres
ISO 374-5:2016(en)
Protective gloves against dangerous chemicals and micro-organisms — Part 5:
Terminology and performance requirements for micro-organisms risks

Key

1 sample

4.2   Sampling for bacteria/fungi penetration testing

The sampling for bacteria/fungi penetration shall be according to EN 374-2:2014, Clause 5.

5   Performance requirement

5.1   General requirements

Protective gloves against micro-organism risks shall comply with the requirements given in EN 420:2009,
Clause 4, Clause 5 and Clause 7.

5.2   Penetration

Protective gloves against virus, bacteria and fungi shall not leak when tested according to EN 374-2:2014,
7.2 and 7.3.

5.3   Protection against viruses

Protective gloves against virus shall be tested according to ISO 16604 Procedure B and shall exhibit no
detectable transfer (<1 PFU/ml) of the Phi-X174 bacteriophage in the assay titre.
ISO 374-5:2016(en)
Protective gloves against dangerous chemicals and micro-organisms — Part 5:
Terminology and performance requirements for micro-organisms risks
5.4   Requirements for different protection types of gloves

The requirements are mentioned in the Table 1.

5.1 5.2 5.3

6   Marking

6.1   General

Marking of protective gloves against micro-organisms shall be in accordance with the marking
requirement for protective gloves in EN 420.

6.2   Marking of gloves protecting against bacteria and fungi

For gloves protecting against bacteria and fungi complying with the requirements stated in 5.4, the
pictogram in Figure 2 shall be used with reference to this part of ISO 374.
Figure 2 — Marking of Glove protecting against bacteria and fungi

6.3   Marking of gloves protecting against viruses, bacteria and fungi

For gloves protecting against viruses, bacteria and fungi complying with the requirements stated in 5.4,
the pictogram in Figure 3 shall be used with reference to ISO 374.
Figure 3 — Marking of gloves protecting against viruses, bacteria and fungi
ISO 374-5:2016(en)
Protective gloves against dangerous chemicals and micro-organisms — Part 5:
Terminology and performance requirements for micro-organisms risks

7   Information supplied by the manufacturer

The information supplied by the manufacturer shall be in accordance with the requirements for
information as defined in EN 420. For protective gloves that are marked offering protection against micro-
organisms and complying with the requirements in 5.4, this shall be stated in the user instructions.
The following warning shall be added that this information does not reflect the actual performance in the
workplace: ”The penetration resistance has been assessed under laboratory conditions and relates only
to the tested specimen.”
If not tested against viruses, the following warning shall be added: “Not tested against viruses”.