Instruction Service Manual: May 2005, Printed in Japan
Instruction Service Manual: May 2005, Printed in Japan
Instruction Service Manual: May 2005, Printed in Japan
TABLE-TOP CENTRIFUGE
INSTRUCTION
and
SERVICE MANUAL
NOTE
■The products being indicated in this Instruction manual are designed for opera-
tors carrying expert knowledge and these products are to be used by these qu-
alified operators observing the indicated precautions for respective purposes.
For persons lacking necessary expert knowledge, these produces may be diffi-
cult to use properly and even danger may be consequent upon the use.
When the aforesaid persons lacking the necessary expert knowledge are using
these products, do so under appropriate supervision and guidance of the quali-
fied operators possessing the necessary expert knowledge.
■Do not distribute this manual within the U.S.A., Mexico, Canada and Australia
as the Products advertised in the manual shall not be distributed in the U.S.A.,
Mexico, Canada and Australia.
For information added or modified after June 2005, please contact your local dealer.
KUBOTA CORPORATION
29-9 HONGO 3-CHOME,BUNKYO-KU,TOKYO 113-0033,JAPAN
[40 Pages]
F30241106
Kubota Corporation ("Kubota") warrants that the instrument covered by this warranty shall be
free from defects in material and workmanship under normal use. Kubota will repair or replace,
free of all charges, the instrument which, within one (1) year after delivery or fifteen (15) months
after shipping, whichever comes earlier is proved to the satisfaction of Kubota to have been
defective at the time of delivery, provided that it does not fall under the exceptions and conditions
specified in this warranty. Such exception and conditions include, but are not limited to, failure
due to natural wear and tear, accident, negligence, alteration, repair, or operation in a manner not
prescribed in the Instruction Manual supplied with the instrument. The foregoing expresses
Kubotas sole warranty with respect to the instrument.
THIS WARRANTY IS MADE IN LIEU OF ANY AND ALL OTHER WARRANTIES AND ALL
IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR
PURPOSE ARE HEREBY DISCLAIMED AND EXCLUDED.
Kubota and its authorized dealers will not be liable for any consequential damages, loss or
expense arising from the improper use of the instrument. Kubota will not honor any other
warranty, which may be given, by its representative or dealer or otherwise which is different from
the warranty given hereunder. This warranty is not assignable and is operative only in favor of the
original customer to whom this warranty is originally delivered.
■“Serious injury" is defined as injuries such as loss of eyesight, burn ( high / low temperature ),
electric shock, fracture of bone, poisoning causing aftereffects, or any other injuries requiring a
long-term medical treatment at hospital.
■“ Non-fatal injury" is defined as burns, electric shock, or any other injuries which does not
require long-term medical treatment at hospital. "Property damage" is defined as expansion
damage related to damage to equipment or other properties.
page page
Safety Instructions
General Notes・・・・・・・・・・・・・・・・・・・・・・ Ⅰ 3-5.Setting the Timer・ ・・・ ・・・・ ・・・・・ ・・ ・3- 8
Usable Rotor ・・・・・・・・・・・・・・・・・・・・・・・ Ⅳ 3-6.Setting the acceleration ・ deceleration
Lifetime of rotors・・・・・・・・・・・・・・・・・・・ Ⅳ ・・・・・・・・・・
・・・・・
・・・・・・・・・・
・・・・・3- 9
Number of times allowed for 3-7.Saving the Memory・・・・
・・・・・・・・・・3-10
autoclaving of rotor・・・・・・・・・・・・・・・・ Ⅴ [1]Saving the Memory・ ・・
・・・・・・・・・・3-10
[2]Memory cancellation・・
・・・・・・・・・・ 3-10
3-8.Setting the Function ・ ・・・・ ・・・・・・・・3-11
Section 1. [1]Setting the Ro tatio n Radius・・・・・・・3-12
Component Name and Explanation [2]Setting the “ SLOW" deceleration
・・・ ・・・ ・・・・・・・・・・・・・・・・・・・ ・・ ・・3-13
1-1.Appearance・・・・・・・・・・
・・・・・・
・・・・・ 1-1 [3]Setting the Sound that informs
1-2.Control Panel ・・・・・・・・
・・・・・・
・・・・・ 1-2 the end of the operation・・・・・
・・・・・3-14
[4]Reverse of setting order of
the Speed an d the Timer ・・
・・・・・3-15
Section 2. [5]Setting the Remin der alarm・・・
・・・・3-16
Installation and Power Supply [6]Setting the Indicators for
the end of the operation ・・・
・・・・3-17
2-1.Unpacking・ ・・ ・・ ・・・ ・・・
・・ ・・・・・・・・・ 2-1 3-9.Calculating Centrifugal Force・・
・・・・3-18
2-2.Place of Installation ・・・・・・・・・・・・・・ 2-1 3-10.Allowable lo ad and
2-3.Movement of centrifuge ・・・・・・
・・・・・2-1 Reduced maximum speed ・
・・・・・3-18
2-4.Power Requirement・・・・
・・・・・・
・・・・・ 2-2
2-5.Grounding・
・・・・・・・・・・
・・・・・・
・・・・・ 2-3
Section 4. Service
Section 5. Troubleshooting
Section 7. Specifications
5-1.Alarm Indicators・ ・・・・・
・・・・ ・・
・・・・・ 5-1
E0・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-1 7-1.Centrifuge・
・・・・・・・・・・
・・・・・
・・・・・・7-1
C1 ・・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2 7-2.Standard Accessories ・・
・・・・・・・・・・・7-1
C2・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
C3 ・・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
C4・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
Section 8. Parts List
C5・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
5-2.Error Indicators・・・・・・・
・・・ ・・・
・・・・・ 5-3
8-1.Recommended Spare Parts・・・
・・・・・・8-1
E1・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-3
E2・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-3
E3・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-3
E4・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-4
E6・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-4
E7・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-4
5-3.Troubleshooting・ ・・・・・
・・・・ ・・
・・・・・ 5-5
Section 6. Rotor
WARNING
(1)Maximum load (5)While the centrifuge is in operation
Do not exceed the maximum load Whilethe centrifugeis in operation,
of rotor and bucket. do not stand closer than 30 cm to
The rotor or bucket used beyond avoid asecondary accident.
the allowable load level can be
damaged, thereby causing an ac-
cident. (6)Lid
Do not open the lid when the rotor
(2)Maximum speed is spinning.
Physical contact with the spinning
Keep the rotor and bucket speed rotor or bucket may cause serious
below the maximum speed. injury.
Excessive speed may cause dam-
age to the rotor, bucket and the (7)Sterilization
centrifuge. The maximum speed
depends on the rotor and bucket Do not perform dry-heat or auto-
strength. clave sterilization with tempera-
tures higher than those specified.
(3)Modification and unspecified parts
Otherwise, the rotor may deterio-
Do not modify, nor use unspeci- rate and cause an accident.
fied parts.
Unauthorized retrofit of the centri-
fuge, rotor or bucket, or use of un- (8)Rotor and drive shaft during rotation
authorized parts for them can re-
sult in accidents. Do not touch rotor and drive shaft
during the rotation.
Physical contact with the spinning
(4)Hazardous material rotor or drive shaft may cause
serious injury.
Do not put any hazardous materi-
al (explosive, chemically active,
organic, or radiation containing (9)Damaged, corroded, rusted or deformed
material, or material contaminated
by pathogenic microorganisms) as Discontinue use of the equipment
a sample of the centrifuge and do when its rotor or the buckets
not place it closer than 30 cm to found to have been damaged,
avoid a secondary accident corroded, rusted or deformed.
should the centrifuge accidentally Otherwise failure may occur.
rotate and contact the material.
CAUTION
Installation
Do not install the centrifuge in a dusty
Do not install the centrifuge on an
place.
inclined, slippery, or unstable sur-
face. Do not install the centrifuge in a place
Violent vibration may occur. with poor ventilation.
Otherwise inside temperature of the cen-
Do not install the centrifuge in a trifuge may rise, resulting in accidents.
place where the temperature is
below 10 or over 40 . Do not install the centrifuge in a place
A place with the ambient tempera- with high humidity (relative humidity 85%
ture beyond 40 can introduce or above).
undesirable build-up of heat inside Leak or accident may occur.
the centrifuge and a place under
10 can cause the centrifuge
malfunction and, as the result, ac-
cidents.
RS-240
RMP-23
RS-1004
Lifetime of rotors
WARNING
Use of rotors beyond the lifetime may lead to breakage of the rotor.
If the rotor is used continuously even after the lifetime of rotors has expired, should
the rotor get damaged, the main unit of the centrifuge suddenly may start to rotate;
this could result in an accident causing injury or death.
When 7 years have passed after the delivery, discontinue operation of the centrifuge to replace the
rotor with a new one.
Earlier replacement, however, is required if any corrosion, lowered strength, flaw or deform due to
incorrect operation is detected on the rotor.
In such case, contact your local dealer and be sure to have the rotor checked before reusing it.
The number of times allowed for autoclaving of each rotor should be deemed as follows.
When the following conditions has been met, discontinue operation of the centrifuge to
replace the rotor with a new part.
Earlier replacement, however, is required if any corrosion, lowered strength, flaw or deform
due to wrong operation is detected on the rotor. In such case, contact your local dealer and
be sure to have the rotor checked before reusing it.
Number of times
Rotor Temperature of autoclave
allowed for autoclaving
You can take advantage of using the “ Autoclave record table ”attached to
the rotor instruction manual.
Hook
Lid
Tachometer port
Refer to Page 4-7.
Chamber
Drive Shaft
Bucket intervention
prevention plate
This plate prevents the touch of
the bucket.
Do not remove this plate, when
you remove the rotor.
“OPEN”button
The lid opens when it is
pushed during the operation
stop with the power on.
Refer to Page 3-4.
S PE
ED
100 rp m
10 0 g T IM
E
mi n
ACC
sec
Front cover
D EC S TA R T
FU NCT IO N
1 2
M EMO R 3
S TO P
1-1
1-2.Control Panel
100 rp
m TIM E
100 g
m in
s ec
S TA R T
AC C
DE C F UN C T I
ON
1 2
3
MEMO
RY
S T OP
1-2
2-2.Place of installation
WARNING
A clearance of 30 cm minimum must be provided around the centrifuge.
If the centrifuge is driven into uncontrolled rotations due to a failure, secondary
damage can result from energy absorbed by the rotation.
CAUTION
Do not install the centrifuge on an inclined, slippery, or unstable surface.
Violent vibration may occur.
When installing this centrifuge, keep 3meters or more away from AM radio.
Noise generated by this centrifuge causes the sound of AM radio deteriorated.
2-3.Movement of centrifuge
WARNING
● Never move the centrifuge while the rotor is rotating or while the rotor is
attached to the centrifuge. Otherwise, the drive shaft may become bent
or the rotor and the bucket may come off, resulting in an accident or
damage to the centrifuge.
● Ensure that rotor and bucket are removed from the centrifuge and that
the power cord is disconnected from the wall socket.
● Moving the position of the centrifuge while the power is turned on may
cause electrification accident or functional failure of the centrifuge.
CAUTION
■ When moving the centrifuge, lift up the bottom of the centrifuge
body by two or more persons using equal forces to move the
centrifuge to the desired place.
■ Do not drop the centrifuge, otherwise damage or injury may occur.
Current requirement 8A 5A
Table 2-1 Rated Voltage, Current and Acceptable Voltage Range and Current Requirement
WARNING
● The power cable alone should be connected to the plug socket.
● Do not use a branched plug socket, which may cause overheating or fire.
2-2
2-5. Grounding
Inlet
NOTE
When using the centrifuge for the
first time, plug the power cable on
the centrifuge into the inlet on the
back of the centrifuge. Power plug
WARNING
Ensure that the ground wire is connected to the grounding terminal.
WARNING
Do not connect the ground cable to the following places:
1. Gas piping
Explosion or fire may occur.
2. Ground cable of the lightning conductor, or telephone cable. Electric
shock may occur in case of thunderbolt.
3. Water pipes
City water pipes may not be adequate as a ground since it may be
connected to plastic pipework.
Ground terminal
Plug Socket
Power Power
(Black) Power plug
(W hite)
Ground wire
(Green-and-yell ow stripe Ground terminal
pattern)
2-3
When using this centrifuge, observe the contents of the Section "General
Notes" being described in the front part of this document and the
precautions given in respective sections.
3-2.Operation
Operation 2. After the “STOP”lamp on the control panel lights, press the “OPEN”button.
Operation 7. Proceed to operation 9 when operating with the same setting values as before.
NOTE
Press the "SPEED/TIME" knob to check the "centrifugal force" of the set
speed or "speed" of the set centrifugal force.
START
min
When the value becomes 0 , the centrifuge
sec automatically decelerates and stops.
Operation 10.
(1)When you want to change the setting (speed, RCF, time) during the operation.
Press the “FUNCTION" key 1 or 2 times on the control panel after changing the
setting, and the flashing of the display is stopped.
The operation becomes shifted to the setting that have
FUNCTION
been changed.
In case that the operation is stopped before the end of the period set by the
timer and is resumed, the operation period after resumption is the remained
period of the operation.
If the knob is pressed before resumption of operation, the operation period
returns to the originally set period.
3-2
When the rotor has stopped, the “STOP" lamp flashes and an sound that informs the end of
the operation is issued.
● The “ Sound that informs the end of the operation ” can be selected from the 5 kinds +
Sound none. Refer to page 3-14.
● The “ Alarm Indicators ” to show the end of the operation can be selected from the 5
kinds. Refer to page 3-17.
Operation 14. After finishing use of the centrifuge, turn "OFF" the "POWER" switch to turn off
the power supply, leaving the lid in opened state.
NOTE
If the power is turned off while a memory being called up and is
then turned on again, the same memory as displayed before the
power cut will be called up and displayed.
Ex. : Turn off the power while“MEMORY 2”called up. Turn on the power,
the“ MEMORY 2”key lamp will light and the parameter stored under
“MEMORY 2”key will be displayed.
3-3
NOTE
Open
The lid is locked in following
cases and will not open even if
the〈OPEN〉button is pressed.
・ When the rotor is spinning.
“OPEN ”button ・ When there is a power failure
S
P
EED
00r pm
1
100g T
IM E
or the power is turned off.
mi n
ACC s
DEC ec START
FNCTION
U
1 3
M2
MEOY
R
ST
OP
WARNING
Do not open the lid while the rotor is spinning.
When a power failure occurs during operation of the centrifuge, the rotor
will naturally decelerate to stop its rotation. If the lid is unlocked forcibly
before the rotor stops, the person can be entangled in the rotor, resulting
in a serious injury or death.
3-4
WARNING
For prevention of electric shock, wait at least 5 minutes before opening the
front lid.
(3)When more than 5 minutes have passed after turning off the power, remove the 2
screws on the left and the right sides of the centrifuge.
(4)Insert a minus driver into the clearance between the front cover and the centrifuge, and
turn the front cover over gently to the front side.
Minus driver
Space
Screws EED
SP
1 pm
(two)
00r
100g TI ME
mi n
AC
C c
se
DEC STA
RT
FUNIO
CTN
1
O23
MEMRY
SP
O
T
Lock plate
Push
Push
“OPEN”button
NOTE
The lid can be closed even
when there is a power failure
or the power is turned off.
3-5
×100 g
SPEED
×100rpm
×100 g
NOTE
● The centrifuge can be started with the speed on the "SPEED" display,
even if the display of value is flashing.
● When you want to change the setting during the operation, press the
“ FUNCTION" key 2 times on the control panel after changing the
setting, and the flashing of the display is stopped.
The operation becomes shifted to the setting that have been changed.
● The setting order of the speed and the timer can be changed.
Refer to page 3-15.
3-6
×100 g
SPEED
×100rpm
×100 g
SPEED TIME
×100rpm min
FUNCTION
×100 g sec
NOTE
● The centrifuge can be started with the speed on the "SPEED" display,
even if the display of value is flashing.
● When you want to change the setting during the operation, press the
“ FUNCTION" key 2 times on the control panel after changing the
setting, and the flashing of the display is stopped.
The operation becomes shifted to the setting that have been changed.
● The setting order of the speed and the timer can be changed.
Refer to page 3-15.
3-7
min
FUNCTION
sec
(2)Pressing “SPEED/TIME" knob changes the settable range of centrifuging time as des-
cribed below. Set to the range as required.
Factory default,the range is set minutes.
① Setting minutes
② Setting seconds
min
Settable range 1-99 seconds
sec
Setting unit 1 second
③ HOLD min
It will continually operate regardless of
sec
the timer. When set to hold, both "min"
and "sec" lamp will be turn off.
NOTE
● In case that the operation is stopped before the end of the period set by the
timer and is resumed, the operation period after resumption is the remained
period of the operation.
If the "SPEED/TIME" knob is pressed before resumption of operation, the
operation period returns to the originally set period.
● The centrifuge can be started at the time when the setting value is flashing at
"TIME" display.
● When you want to change the setting during the operation, press the
“ FUNCTION" key on the control panel after changing the setting, and the
flashing of the display is stopped.
The operation becomes shifted to the setting that have been changed.
● The speed and the timer can be set in this sequence or vice versa.
As for the change of the setting, please refer to page 3-15.
3-8
SL OW
RAPID acceleration
acceleration It changes to the SLOW characteristic
[Ex.] from RAPID at 1000rpm.
SPEED
1000rpm
TIME
Natural dece lerati on
RAPID SLOW
deceleration deceleration
NOTE
You can set the natural deceleration starting speed N.
Refer to page 3-13.
ACC
ACC:SLOW (“ SLOW ” acceleration)
DEC
DEC:SLOW (“ SLOW ” deceleration )
3-9
NOTE
The parameters necessary for operation (speed, RCF, time, acceleration /
deceleration, etc.) can be stored as programs with MEMORY 1~3 keys.
NOTE
Even if the power is turned off, the parameters set at the time of power off
remain stored.
The last setting is displayed and can be operated.
[2]Memory cancellation
Pressing the “Function ”key or the “ACC/DEC”key, the normal setting mode
will be selected.
3-10
FUNCTION
(1)Pressing the "SPEED/TIME" knob while holding
down the "FUNCTION" key .
SPEED TIME
(2)The used number of times of the ×100rpm min
centrifuge is displayed. ×100 g sec
[ Ex.]186 times
SPEED TIME
(4)Setting the "SLOW" deceleration. ×100rpm min
Refer to Page 3-13.
×100 g sec
[Ex.]300rpm
SPEED TIME
(5)Setting the Sound that informs the
×100rpm min
end of the operation.
Refer to Page 3-14. ×100 g sec
[Ex.]1
SPEED TIME
(6)Reverse of setting order of the ×100rpm min
Speed and the Timer. ×100 g sec
Refer to Page 3-15.
[Ex.]0
SPEED TIME
×100rpm min
(7)Setting the Reminder alarm.
Refer to Page 3-16. ×100 g sec
[Ex.]ON
SPEED TIME
(8)Setting the Indicators for the end of ×100rpm min
the operation. ×100 g sec
Refer to Page 3-17.
[Ex.]1
■ After setting any one of the above, please return the display to the previous indication of
"SPEED" and "TIME".
To do above, press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
3-11
(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the left side of
the “SPEED”display.
The “SPEED”and “TIME”display indicates the current setting.
Unit : mm
Refer to Page 3-11.
[Ex.]The rotation radius is 146 mm.
SPEED TIME
×100rpm min
×100 g sec
(3)Turn the “ SPEED/TIME”knob to set to the rotation radius of the rotor bucket to be
used for operation.
The range is set from 50 to 150 mm.
SPEED TIME
×100rpm mi n
×100 g sec
FUNCTION
NOTE
The rotation radius can not be stored in the memory, when the power
supply is turned off without returning the display to the previous
indication.
3-12
TIME TIME
(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.
(2)Pressing the "SPEED/TIME" knob several times and let indicate " "on the "TIME"
display.
The "SPEED" display indicates the current setting of the natural deceleration speed.
Unit : rpm
Refer to Page 3-11.
[Ex.] Setting is the 0 rpm.
SPEED TIME
×100rpm min
×100 g sec
×100 g sec
(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting ( Natural
deceleration speed) is saved.
FUNCTION
3-13
The “ Sound that informs the end of the operation ” can be selected from the 5
kinds + Sound none.
Use this function to identify each “ Sound that informs the end of the operation ” when
you use more than one centrifuges.
Factory default : [ 1 ]
(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.
(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display. The "SPEED" display indicates the current setting of the sound that informs.
Refer to Page 3-11.
[Ex. ] The “ Sound that informs the end of the operation ” 1.
SPEED TIME
×100rpm min
×100 g sec
(3)Turn the "SPEED/TIME" knob to change the "Sound that informs the end of the opera-
tion".
Pressing the "FUNCTION" key makes the selected buzzer sound once.
The "Sound that informs the end of the operation" can be selected from 1, 2,3, 4,
5 of 5 kinds + 0 (Sound none).
SPEED TIME
×100rpm min
×100 g sec
(4) Then, press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting ( Sound
that informs the end of the operation) is saved.
FUNCTION
3-14
(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.
(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display. The "SPEED" display indicates the current setting of the number (see the figure
below). Refer to Page 3-11.
[Ex.]Numbers 0
SPEED TIME
×100rpm mi n
×100 g sec
Numbers Contents
0 「Speed / RCF」→「Timer」
SPEED TIME
×100rpm min
×100 g sec
(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting (Numbers)
is saved.
FUNCTION
3-15
(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.
(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display.The "SPEED" display indicates the current setting of the reminder on or off.
(see the figure below)
Refer to Page 3-11.
[Ex.]Reminder alarm : ON
SPEED TIME
×100rpm min
×100 g sec
SPEED TIME
×100rpm min
×100 g sec
(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting (reminder
on or off) is saved.
FUNCTION
3-16
(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.
(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display. The "SPEED" display indicates the current setting of the number (see the figure
below). Refer to Page 3-11.
[Ex.]Number : 1
SPEED TIME
×100rpm min
×100 g sec
2 [88][88]flashing
3 [--][--]flashing
4 [E][nd]flashing
SPEED TIME
×100rpm min
×100 g sec
(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting (Numbers)
is saved.
FUNCTION
3-17
Please refer to the Nomograph for Centrifugal Force Calculation at the last page of this
manual.
NOTE
As for rotation radius, please refer the page 6-4, 6-5, 6-10 and 6-12. The
maximum centrifugal force being indicated in the said pages is calculate by
substituting the maximum radius of gyration of the rotor for the radius of
gyration of each rotor (cm) and by rounding the first digit above the decimal
point of the calculation result.
By setting the rotation radius, an accurate speed and centrifugal force can be
switched. Factory default at the time of delivery is 14.6 cm.
Refer to page 3-12.
WARNING
Do not exceed the maximum speed and the allowable load of rotor and
bucket.
An excessive speed or overload may cause a damage to the rotor, bucket
and the centrifuge.
If the load exceeds the limit, reduce the maximum speed as shown in the
formula 3-2 and set the actual speed below the reduced maximum speed.
[ formula 3-2 ]
Allowable load (gram)
Reduced maximum speed(rpm )= Maximum speed(rpm )×
Actual load (gram)
(1)When the gravity of sample is 1.2 or higher or when a special tube is used,
check the allowable load of the rotor and bucket.
(2)Regarding allowable load on rotors and buckets at the maximum speed refer to the
appropriate Specification Table for rotor in use.
(3)The allowable load includes the weight of sample, cushions, adaptors, tubes, caps,
and racks. (The weight of bucket is not included in the allowable load.)
3-18
Check that all knobs, If any do not operate correctly, contact your local
displays, lamps and switches dealer for an inspection.
operate correctly. Refer to Page 1-1 and 1-2.
Check that the centrifuge is
placed horizontally. Refer to page 2-1.
4-1
4-2.Monthly Inspections
[1] Inspect the rotor
WARNING
Perform a monthly careful inspection of the rotor appearance. Check for
any deformation or damage, including the interior and bottom of the holes.
If any abnormality whatsoever is found, there may be significant damage or
corrosion of the rotor that could lead to serious damage or accidents
involving physical injury. Stop operation at once, put "DO NOT USE" labels
on the rotor and centrifuge lid, and contact your local dealer to arrange for
authorized service.
4-3.
Annual Inspection
Annual Inspection (fee required)
For the following inspection items, please call your local dealer and set up an
appointment for a periodical inspection.
4-2
Cleaning the chamber interior according to the following procedure will help
prevent rust.
1) Wipe off any dirt or residue using a cloth moistened with a small quantity of a neutral
detergent.
2) Wipe away any detergent residue using a cloth dampened with water, and then dry the
inside of the chamber.
3) To prevent rust, we recommend the use of common anti-rust agents (such as CRC-556)
only if such will not affect the samples being centrifuged.
Wipe the dirt off using a cloth containing a small quantity of neutral detergent.
[NOTE ]
Under no circumstances should any rust inside the chamber be removed by using
sandpaper or abrasive agents, since if the interior surfaces are scratched, this will
only increase the likelihood of additional rust developing.
CAUTION
Do not use detergents exceeding a pH range of 5 - 8, or chlorinated
detergents normally used for washing.
Corrosion may damage the rotor, bucket, or tube rack, resulting in damage
to the centrifuge that may lead to serious additional damage or accidents
involving physical injury.
NOTE
Do not allow any spilt samples to remain on any surfaces, otherwise rust
or corrosion may occur. Also, if sample spillage is left between the rotor
and shaft, later detachment of the rotor may become problematic.
1) If sample spillage has occurred, remove the rotor, buckets, and tube rack from the
centrifuge, and wash the affected items with a neutral detergent and warm water.
Then rinse the items with the distilled water and thoroughly dry them before use.
2) If water has accumulated inside the rotor, place the rotor with its bottom side up and allow
it to dry completely.
3) If a sample has spilled onto the drive shaft, wipe it off using a cloth moistened with a
small amount of a neutral detergent and then clean away all detergent traces using a cloth
moistened with water. Then, dry the surfaces completely before using the machine.
WARNING
Do not heat the rotor, buckets, or tube rack above 100 ℃ for sterilization
or disinfection purposes.
Also, do not use an autoclave for dry heat sterilization, since excessive
heat may deleteriously affect the strength of the rotor, buckets, or tube
rack, resulting in breakage of the rotor, buckets, or tube rack, causing
serious damage or accidents involving physical injury.
Yet, You can conduct up to 50 times of autoclave sterilizations of the
RMP-23 rotor and bucket at 121 ℃ and 100 times of autoclave sterilizations
of the RS-1004 rotor and bucket at 121 ℃ .
4-5.Greasing
CAUTION
Be careful to maintain lubrication by ensuring that a proper amount of grease is
present in all bucket grooves (section "A" in the schematic diagram below). A
lack of grease may allow violent vibration to occur, resulting in damage to the
centrifuge.
NOTE
RMP-23
S70284
RS-1004
Trunnion pin
Rotor yoke
4-5
Circuit protector
(2)After the rotor stops completely, open the lid and check the rotor, following
the procedure given in [3 - 3. Opening and Closing the lid 2. Opening the lid during
power failure] on Page 3 - 4.
(3)Close the lid , and then push the sticking out white portion until it becomes unseen.
4-6
Reflective tape
(3)After turning the power ON, set the photoelectric tachometer so that its tip is right above
the tachometer port on the lid. The photoelectric tachometer can then measure the actual
rotation rate.
Photoelectric tachometer
Tachometer port
Lid
Centrifuge
Do not apply force to the tachometer port window from inside the lid.
4-7
(1)If you have pertinent information on your handling of biohazards, fill out the
items below, and affix this information to the centrifuge.
2. Sample
3. Required measures against the biohazard.
Prior to sending back our products (including accessories) for repair or for other
reasons, be sure to remove any and all contaminants.
The elimination of contaminants from the equipment is aimed to safeguard the persons
who will inspect and repair the returned products at our facilities.
4-9
Name:
Company / Institute:
Department:
Accessories :
Date when contamination elimination was carried out.
Contaminants:
Method used:
Dealer notification:
Signature:
4-10
(2)When any of the alarms become activated during ope ration (whe n the rotor is spin-
ning), the rotor immediately dece lerates and stops.
[Ex.]
Control panel “TIME"display
TIME
min
sec
NOTE
The equipment is inoperative while any of the alarm indicators is turned on.
Cont’
d. on next page.
5-1
C5 following.
"Alarm Indicators" and "Number of times of rotor use" at the time of rotor inspection
Each "Alarm Indicator" is displayed at the time when the number of times of rotor use reaches
the figures shown below.
C1 50,000~50,050
C2 80,000~80,050
C3 100,000~109,999
C4 110,000~119,999
C5 From 120,000
5-2
5-2.Error Indicators
(1)When trouble occurs during operation, the “ TIME" display flashes to show the error. The
error indicators are mentioned in the table below.
(2)When any of the error indicators turn on during ope ration (when the rotor is spinning),
the rotor immediately dece lerates and stops.
[Ex.]“TIME"display
Control panel
TIME
min
sec
NOTE
The equipment is inoperative while any of the error indicators is turned on.
Speed error
This code appears when the After the rotor stops spinning, turn
off the power supply once and turn
equipment did not start its
on the power after about 30
E7 ope ration within 5 sec onds after
seconds.
the "START" key was pressed by
If the error recurs, contact your
such causes as electric c ircuit nearest dealer for inspection.
failures.
5-4
5-3.Troubleshooting
Is the power plug disconnected Insert the power plug into the
1. The “STOP” from the power outlet? socket.
key lamp does
not lit even if the Is power supplied to the socket or
Inspect the power supply.
“POWER ” knife switch?
switch is turned
on. Is the power supply voltage Use a power supply in the correct
correct? (Note1) voltage range.
2. The power is
turned off imme-
diately even if Is the power supply voltage Use a power supply in the correct
the “ POWER ” correct? (Note1) voltage range.
switch is turned
on.
“ ”
Is the “POWER”switch turn on? Pull up the POWER switch to
turn the power on.
5-5
If the error still remains even after the troubleshooting is made and the action has been taken, or
in case the following phenomena are occurring, stop the operation.
NOTE
When operation centrifuge is interrupted by some abnormality, failure or
for repairs, always turn off the “POWER”switch and attach “DO NOT
USE ” stickers to the rotor and the centrifuge before contacting your
nearest dealer.
5-6
CAUTION
Make sure that the rotor yoke is firmly fastened to the drive shaft
with the rotor locking nut.
Since the rotor is not fastened onto the shaft when thes e parts are loose,excessive
vibrations will occur and damage the equipment and the rotor may fly off inflicting
physical injury.
▲
and then tighten by turning the mark indication to
rotor knob clockwise. align with the drive
pin at 2 places.
Drive pin
Center axis of rotor
Groove
If the position of the pin mark of the rotor does not align with the drive pin,
▲
the groove at the bottom surface of the rotor and drive pin do not fit.
In such a case, reinstall the rotor.
6-2
▲
2.To install the rotors, match the pin mark
of the rotor with the pin of the drive shaft.
Rotor locking nut
3. Fix the rotor by tightening the rotor Rotor yoke
locking nut. Rotate the nut clockwise
using the T-type box wrench etc.
▲
mark indication to
4. After installation of the rotor, hold the align with the drive
pin at 2 places.
rotor yoke and attempt to move it up and
down to ensure that there is no play.
Screw
Drive pin Drive shaft
Center axis of rotor Drive pin
Bucket interven-
Drive shaft tion prevention plate
Groove
(NOTE)
T-type box wrench will be attached to the
Do not start the operation when rotor as standard accessary, when the
the rotor locking nut is removed. second rotor is purchased.
If the drive pin of the drive shaft is not set in the groove of the rotor bottom
surface, the bucket intervention prevention plate is not be fixed and moves
up and down.
In such a case, re-install the rotor.
6-3
Maximum R C F ( × g) *1 2,
610
Nomi- Maxi-
nal Num- Allow- mum Allow-
Capa- ber Tube Sizes able Tube rack / Radius able
city of Tubes Dia. × Length Tubes Speed R C F Bucket Adaptor Cushion R load
ml Tube Material mm Form Code No. rpm ×g Code No. Code No. Code No. cm gram
*2 *3 *5
2 48 PP 9.
5~11× C 1.
5~2ml 950 053-5
1, 040 055-48
30 10.
9 50
36~60 R micro tube Carrier
024-18
80
GL 12.
5~13.
3× 053-5 030
6 40 PL 36~80
C
R - 1,
970 Carrier 055-48
20 11.
0 100
Becton,
5 Dickinson
GL 12.5~13.
3× Hemolyse tube
24 PL 46~105 R 430 053-58
2, 50 055-0290 024-18
90 13.
6 120
Carrier
10 052-6320
10/15 4 2,
500 053-4990 055-7400 14.
0 100
GL 15~17.
2× C 052-6360 024-1400
86~110 R 052-6330 4,
000
15 4 2,
540 053-4910 14.
2
30
Conical tubes 053-4910 Use bucket
15 4 PL 17×121 C 2,
580 without 14.
4
FALCON etc. *4
cushion.
VENOJECT Ⅱ
15~17.
2×
etc. -
10/15 8 GL Hemolyse tube 2,
520 053-4900 024-14
00 14.
1
86~110 C 052-6360
R 052-6330 60
*1 The Max. RCF is the calculation value of the Max. speed for the rotation radius of 14.6cm.
The value of the RCF that is indicated to the display is showing the value that cut the lower 2 digits.
Refer to Page 3-12.
*2 PP : Polypropylene GL : Pyrex Glass PL : Plastic
*3 C : Conical R : Round type
*4 Use a bucket after removing cushion.
*5 Allowable load per bucket.
This load includes the weight of the sample, adaptor, tube rack, cushion, tube, cap, etc.
The weight of bucket is not included in the allowable load.
6-4
Nomi- Maxi-
nal Num- Allow- mum Allow-
Capa- ber Tube Sizes able Tube rack / Radius able
city of Tubes Dia. × Length Tubes Speed R C F Bucket Adaptor Cushion R load
ml Tube Material mm Form Code No. rpm ×g Code No. Code No. Code No. cm gram
*2 *3 *5
VENOJECT Ⅱ
15~17.
2× etc.
10/15 16 GL Hemolyse tube 2,
500 053-4980 024-1400 14.
0 120
86~110 C 052-6360
R 052-6330
VENOJECT Ⅱ
15~17.
2× etc.
10/15 24 GL C Hemolyse tube 2,
490 053-7340 024-2610 13.
9 500
89~105
R 052-6
*6
330
-
VENOJECT Ⅱ
50 4 GL 27~37× R etc. 4,
000 2,
540 053-4990 024-3190 14.
2 100
95~110 Hemolyse tube
052-6370
024-1480
50 4 30×117 2,
560 *8 14.
3
Conical tubes
15 PL 17×121 C 2,
610 053-5010 14.
6 80
FALCON etc.
055-6010
4
*7 -
50 30×117 2,
500 14.
0
6-5
[2]Setting Buckets
CAUTION
The same type buckets must be provided to every (4places) rotor yoke.
If not positively held in place, the rotor or centrifuge can be damaged, thereby
causing accidents.
Correct Wrong
Different types of
buckets are used.
Not all locations are provided
with a bucket.
Dummy
Correct Put the dummy in to
keep the l oad
balanced.
Wrong
CAUTION
■ Distribute the tubes symmetrically about the center of the bucket.
・Appropriate balance is lost and violent vibrations can result if tubes are incorrectly
arranged. And, unexpected accidents can result from such violent vibrations.
・The settling chamber may be tilted since the tubes can't be maintained horizontal
during rotation.
・When you don't have enough number of tubes, dummy tubes shall be used to
maintain symmetry of the both sides.
■ Keep the load balanced symmetrically within 10 gram.
6-6
[4]Cushions / Adapter
When glass tubes are used, be sure to use the cushion to avoid damage.
Insert the cushion into the tube rack so that its concave side faces
upward. Glass tube
If a glass tube is damaged, replace the cushion.
Bucket
If broken pieces of the glass tube are contained in the cushion, the
glass tube will be broken again.
Cushion
① Place a cushion in the bucket with the Cushion for 15ml Glass tube
concave surface facing upward. Code No.024-2610
(Bucket Code No.053-7340)
Code No.024-1400
② Fully insert the cushion to the bottom of the (Bucket Code No.053-4990
hole using a glass tube. 053-4910, 053-4900
(the edges of the cushion bend to prevent it 053-4980)
Bucket
6-7
Carrier Cushion
6ml Glas s tube
Code No. CodeNo.
053-5040
024-1880
053-5030
15 ml conical tube
50 ml conical tube
Bucket Bucket
Code No.053-5010 Code No.053-5010
Adaptor Adaptor
Code No.055-6010 Code No.055-6010
6-8
Bucket
Code No.053-5010
6-9
Nomi- Maxi-
nal Num- Mate- Allow- mum Allow-
Capa- ber rial Tube Sizes able Tube rack Radius able
city of of Dia. × Length Tubes Speed R C F Bucket / Adaptor Cushion R load
ml Tube Tube mm Form Code No. rpm ×g Code No. Code No. Code No. cm gram
*2 *3 *4
GL 12.5~13.
5× Becton, S13041
5 16 PL 70~110 R Dickinson 2,
500 *5 S14033 14.
0
Hemolyse tube
GL 11.8~12.
8×
5 20 PL 65~85 R RIA tube 2,
020 S1300
9 - 11.
3
GL 11.5~12.
5×
7 20 PL 95~105 VENOJECT Ⅱ 2,
400 S13004 - 13.
4
R etc.
7 20 GL 11.5~12.
5× Hemolyse tube 500 S1304
2, 1 S14029 14.
0
PL 65~105 *5
Conical tubes
-
15 4 PL 16.
5×120.
6 C 560 S1315
2, 0 S13060 14.
3
FALCON etc. *6
15 16 PL
15.2~16.
80~11
2×
0 R - 2,
500
S13041
*5 S14030 14.
0
Conical tubes
15 8 PL 16.
5×120.
6 C
FALCON etc.
2,
560 S1315
2 - 14.
3
16~17×
15 16 PL 90~105 R - 4,
000 2,
400 S1301
2 - 13.
4 350
23~24×
25 4 GL 95~100 2,
400 S1309
7 S17925 - 13.
4
R S15025 Cushion is
25/30 4 GL 24~25.
3× 500 S1304
2, 1 S14031 included with 14.
0
75~105 *5
this tube rack.
Conical tubes S13150
50 4 PL 29.
5×117 C
FALCON etc.
2,
590 *6*7 - - 14.
5
34~35×
50 4 GL 95~100 R 2,
400 S1309
7 S17950 - 13.
4
S15050
34~35× Cushion is
50 4 GL 70~105 500 S1304
2, 1
*5 S14032 included with 14.
0
this tube rack.
S16051
44.5~45.
5×
100 4 GL 95~105 R S1510
0 2,
400 S13097 - Cushion is 13.
included with
4
this bucket.
*1 The Max. RCF is the calculation value of the Max. speed for the rotation radius of 14.5cm.
The value of the RCF that is indicated to the display is showing the value that cut the lower 2 digits.
Refer to page 3-12.
*2 GL : Pyrex Glass PL : Plastic
*3 C : Conical R : Round type
*4 Allowable load per bucket.
This load includes the weight of the sample, adaptor, tube rack, cushion, tube, cap, etc.
The weight of bucket is not included in the allowable load.
*5 Sealing cap No.S17140 is included with this Bucket.
*6 Sealing cap No.S17151 is included with this Bucket.
*7 When using the adapter (S13060), 4 tubes of 15ml conical tube can be used.
6-10
[2]Setting Buckets
CAUTION
The same type buckets must be provided to every (4places) rotor yoke.
If not positively held in place, the rotor or centrifuge can be damaged, thereby
causing accidents.
Correct Wrong
Different types of
buckets are used.
Not all locations are provided
with a bucket.
[3]Tube distribution method
CAUTION
■ Distribute the tubes symmetrically about the center of the rotor.
Improper distribution will cause violent vibration resulting in damage to the
centrifuge, or the rotor may come off.
■ Keep the load balanced symmetrically within 10 gram.
Correct Dummy
Put the dummy in to
keep the l oad
balanced.
Wrong
[4]Standard accessories
(1)Grease (SIGMA Parts No.70284) ・
・・・・・・・・・・・・・1Pc.
(2)Rotor knob・・・
・・・・ ・・ ・・・・・・・・・・・・・・・
・・・ ・
・・ ・・
・1Pc.
6-11
R1
2~6
96 *2 PL Bucket is
86×128×52 4,
000 1,
790 included 10 345
with this
96 2 PL rotor.
*1 The Max. RCF is the calculation value of the Max. speed for the rotation radius of 10 cm.
The value of the RCF that is indicated to the display is showing the value that cut the
lower 2 digits. Refer to Page 3-12.
*2 The maximum speed drops when the step number increases, although a micro plate is
possible to piling 3 steps. Refer to Page 6-15.
*3 PL : Plastic
*4 Allowable load per bucket. This load includes the weight of the sample, plate,
adaptor etc.
The weight of bucket is not included in the allowable load.
Because of the size of the micro plate,the radius of rotation differs between the center and
the both ends with respect to the rotation axis ,
resulting in the difference in the centrifugal
.
force The centrifugal force of the place A (cm) Distant from the center of a bucket can be
calculated in accordance with the formula, where the rotation radius R shall be substituted
with the Rx to be obtained by the formula mentioned below.
R(
X cm)= R1
( cm)+A(
2 2
cm)
RX:Actual rotation radius in a position which is A cm away from the center of the
bucket.
6-12
CAUTION
Check the trunnion pin (4 places) is not loosened.
If the trunnion pin is loosened, the rotor or centrifuge can be damaged,
thereby causing accidents.
Trunnion pin
[3]Setting Buckets
CAUTION
The same type buckets must be provided to every (2 places) rotor yoke.
If not positively held in place, the rotor or centrifuge can be damaged,
thereby causing accidents.
Correct Wrong
6-13
[4]Distribution of samples
CAUTION
■ Keep the load (of the sample) balanced.
If an appropriate balance is not provided, unexpected accidents can
result from a damaged rotor or centrifuge.
■ Keep the load balanced symmetrically within 5 gram.
Correct Wrong
● Sample
[5]Setting plate
CAUTION
Use the same plates.
The wrong arrangement will cause imbalance and resulting in damage
to rotor, bucket or the centrifuge.
6-14
WARNING
■ When pile up the micro plate to each bucket in 3 steps, use it after
the sufficient test for the strength of the plate is made.
The more the micro plates are piled up, the easier the plate at the
lower position becomes damaged.
■ When deep well plate is used,please ask the plate maker for the
allowable RCF of the deep well plates.
■ Allowable weight of micro plate and cell culture plate put in the
bucket is 345 gram. If it exceeds 345 gram, rectify reduced
maximum speed by the following formula.
If it is not used at the rectified speed, the rotor and the bucket will
be damaged, causing the accident.
[7]Standard accessories
Grease (SIGMA Parts No.70284) ・
・・・
・・・
・・・・
・・・・
・・・・
・・・・
・・・1Pc.
6-15
7-1.Centrifuge
Max. speed 4,000 rpm
Max. RCF 2,610 × g
Max. Capacity 400ml
Invertor Microprocessor control, Speed, Centrifugal force, time,
Control system
acceleration / deceleration, 3 memory
Acceleration/Deceleration Rapid, Slow, 4 ways of switching
Lid open, Imbalance, Over speed, Function for detecting an
Alarm display occurrence of electrical abnormality in speed sensor, invertor, Lid
rock, Motor
Speed setting Digital display, 200-4,000 rpm, in 100 rpm steps
Speed indication Digital display, 0-4,300 rpm, in 100 rpm steps
Digital display, in 100 × g steps
RCF setting
10-2,600 × g(The centrifugal force differs by the rotation radius. )
Digital display, in 100 × g steps
RCF indication
0-3,000 × g(The centrifugal force differs by the rotation radius. )
From 1 second to 99 seconds (1 second step)
Timer setting range
1 minutes to 99 minutes(1 minutes step) HOLD
Setting the Sound that
informs the end of the 5 kinds + Sound none
operation
Rated voltage and Rated 100-115 V 50/60 Hz 3A
current 200-240 V 50/60 Hz 1.3 A
100-115 V 180 W 0.65 MJ/h
Total heat produced
200-240 V 170 W 0.62 MJ/h
Dimensions, Weight 35 (W) × 42(D) × 32(H) cm, 24 kg
Safety regulation IEC 61010-2-020, CLASS Ⅰ
Temperature : 10 to 40 ℃
Operation environment Atmospheric pressure : 70 to 106 kpa (700 to 1,060 mbar)
Humidity : 30 to 85%
7-2.Standard Accessories
1. Instruction manual・
・・
・・
・・
・・
・・
・・
・・
・・1 Copy 2. Power cable・
・・
・・
・・
・・
・・
・・
・・
・・
・・
・・
・・1 Pc.
7-1
8-1
C S
Calculating Centrifugal Force 3-18 sec 3-8
Centrifugal force(×g) 3-7 Sound that informs 3-14
Circuit Protector 4-6 the end of the operation
Cleaning &Sterilization 4-3, 4-4 Speed 3-6
Contaminant Elimination 4-9, 4-10 START 1-2, 3-2
Control Panel 1-1, 1-2 STOP 1-2, 3-2
D T
DECEL 1-2, 3-9, 3-13 Tachometer port 1-1, 4-7
G Timer 3-8
Greasing 4-5 U
Grounding 2-3 Using rotor Ⅳ
H Rotor
HOLD 3-8 RMP-23 6-3, 6-11, 6-12, 6-13, 6-14
O C4 5-2
Opening and Closing the lid 3-4, 3-5 C5 5-2
Operation 3-1, 3-2, 3-3 Error Indicators
P E1 5-3
Place of Installation 2-1 E2 5-3
Power Requirement 2-2 E3 5-3
E4 5-4
E6 5-4
E7 5-4