Instruction Service Manual: May 2005, Printed in Japan

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May 2005, Printed in Japan

TABLE-TOP CENTRIFUGE

INSTRUCTION
and
SERVICE MANUAL

To ensure proper operation of the centrifuge, be sure to read this manual


carefully before operating it.
Also, keep this manual handy so that you can refer to it at any time.

NOTE
■The products being indicated in this Instruction manual are designed for opera-
 tors carrying expert knowledge and these products are to be used by these qu-
 alified operators observing the indicated precautions for respective purposes.
 For persons lacking necessary expert knowledge, these produces may be diffi-
 cult to use properly and even danger may be consequent upon the use.
 When the aforesaid persons lacking the necessary expert knowledge are using
 these products, do so under appropriate supervision and guidance of the quali-
 fied operators possessing the necessary expert knowledge.
■Do not distribute this manual within the U.S.A., Mexico, Canada and Australia
as the Products advertised in the manual shall not be distributed in the U.S.A.,
Mexico, Canada and Australia.

For information added or modified after June 2005, please contact your local dealer.

KUBOTA CORPORATION
29-9 HONGO 3-CHOME,BUNKYO-KU,TOKYO 113-0033,JAPAN

[40 Pages]
F30241106

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WARRANTY

Kubota Corporation ("Kubota") warrants that the instrument covered by this warranty shall be
free from defects in material and workmanship under normal use. Kubota will repair or replace,
free of all charges, the instrument which, within one (1) year after delivery or fifteen (15) months
after shipping, whichever comes earlier is proved to the satisfaction of Kubota to have been
defective at the time of delivery, provided that it does not fall under the exceptions and conditions
specified in this warranty. Such exception and conditions include, but are not limited to, failure
due to natural wear and tear, accident, negligence, alteration, repair, or operation in a manner not
prescribed in the Instruction Manual supplied with the instrument. The foregoing expresses
Kubotas sole warranty with respect to the instrument.
THIS WARRANTY IS MADE IN LIEU OF ANY AND ALL OTHER WARRANTIES AND ALL
IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR
PURPOSE ARE HEREBY DISCLAIMED AND EXCLUDED.
Kubota and its authorized dealers will not be liable for any consequential damages, loss or
expense arising from the improper use of the instrument. Kubota will not honor any other
warranty, which may be given, by its representative or dealer or otherwise which is different from
the warranty given hereunder. This warranty is not assignable and is operative only in favor of the
original customer to whom this warranty is originally delivered.

Use of Model 2420


Model 2420 can be used for preprocessing in in-vitro analysis, which breaks down blood or urine
samples containing plasmas or cells. Accordingly, it is not designed to connect directly to a
patient's body.
Do not use the centrifuge for separation of any hazardous material (explosive, chemically active,
organic, or radiation containing material, or material contaminated by pathogenic microorganisms)
or oil.

         Copyright C 1995 KUBOTA Manufacturing Corporation

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Safety Instructions
The centrifuge and the manual indicate important information in order to ensure safe
operation of the centrifuge and to prevent physical injuries and property damages.
Be sure to understand the meanings of the following indications and follow the
instructions.

1. Explanation of indication marks


Indication Meaning

It is a possibility of serious accident resulting in


WARNING death or serious injury.

It is a possibility of accident resulting in slight or


CAUTION non-fatal injury or property damage.

■“Serious injury" is defined as injuries such as loss of eyesight, burn ( high / low temperature ),
electric shock, fracture of bone, poisoning causing aftereffects, or any other injuries requiring a
long-term medical treatment at hospital.
■“ Non-fatal injury" is defined as burns, electric shock, or any other injuries which does not
require long-term medical treatment at hospital. "Property damage" is defined as expansion
damage related to damage to equipment or other properties.

2. Explanation of pictorial marks


Pictorial marks Meaning
Indicates prohibition (things you must not do).
Details are shown near the mark, using illustration or
sentences.
Indicates compulsion (things you have to do).
Details are shown near the mark, using illustration or
sentences.
Indicates caution, warning and danger.
Details are shown near the mark, using illustration or
sentences.

This label indicates the risk of electric shock.


Touching this attached part will cause an electric shock.

This label indicates a hot section.


Touching this attached part will cause an burn.

Indicates that the power is on.


It is indicated on the power switch and the circuit breaker.

Indicates that the power is off.


It is indicated on the power switch and the circuit breaker.

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Table of Contents

page page
Safety Instructions
General Notes・・・・・・・・・・・・・・・・・・・・・・ Ⅰ 3-5.Setting the Timer・ ・・・ ・・・・ ・・・・・ ・・ ・3- 8
Usable Rotor ・・・・・・・・・・・・・・・・・・・・・・・ Ⅳ 3-6.Setting the acceleration ・ deceleration
Lifetime of rotors・・・・・・・・・・・・・・・・・・・ Ⅳ ・・・・・・・・・・
・・・・・
・・・・・・・・・・
・・・・・3- 9
Number of times allowed for 3-7.Saving the Memory・・・・
・・・・・・・・・・3-10
autoclaving of rotor・・・・・・・・・・・・・・・・ Ⅴ [1]Saving the Memory・ ・・
・・・・・・・・・・3-10
[2]Memory cancellation・・
・・・・・・・・・・ 3-10
3-8.Setting the Function ・ ・・・・ ・・・・・・・・3-11
Section 1. [1]Setting the Ro tatio n Radius・・・・・・・3-12
Component Name and Explanation [2]Setting the “ SLOW" deceleration
・・・ ・・・ ・・・・・・・・・・・・・・・・・・・ ・・ ・・3-13
1-1.Appearance・・・・・・・・・・
・・・・・・
・・・・・ 1-1 [3]Setting the Sound that informs
1-2.Control Panel ・・・・・・・・
・・・・・・
・・・・・ 1-2 the end of the operation・・・・・
・・・・・3-14
[4]Reverse of setting order of
the Speed an d the Timer ・・
・・・・・3-15
Section 2. [5]Setting the Remin der alarm・・・
・・・・3-16
Installation and Power Supply [6]Setting the Indicators for
the end of the operation ・・・
・・・・3-17
2-1.Unpacking・ ・・ ・・ ・・・ ・・・
・・ ・・・・・・・・・ 2-1 3-9.Calculating Centrifugal Force・・
・・・・3-18
2-2.Place of Installation ・・・・・・・・・・・・・・ 2-1 3-10.Allowable lo ad and
2-3.Movement of centrifuge ・・・・・・
・・・・・2-1 Reduced maximum speed ・
・・・・・3-18
2-4.Power Requirement・・・・
・・・・・・
・・・・・ 2-2
2-5.Grounding・
・・・・・・・・・・
・・・・・・
・・・・・ 2-3
Section 4. Service

Section 3. Operation 4- 1.Daily Inspection・・・・・・


・・・・・・・・・・・4-1
4- 2.Monthly In sp ections ・・
・・・・・・・・・・・4-2
3-1.Cautions of Operation・・・・・・・・
・・・・・3-1 4- 3.Annual Inspection・・・・
・・・・・・・・・・・4-2
3-2.Operation ・・・・・・・・・・
・・・・・・
・・・・・ 3-1 4- 4.Cleaning and Sterilization ・・・
・・・・・・4-3
3-3.Opening and Closing the lid ・・・
・・・・・ 3-4 [1]Cleaning the chamber interior ・・・・・・4-3
[1]Turning on the power and opening [2]Cleaning the rotor, buckets and
the lid・・・・
・・ ・・ ・・・・・ ・・・・・・ ・・・ ・・ ・ 3-4 tube rack ・ ・・ ・・ ・・・・ ・ ・・・・・ ・・・・ ・・・4-4
[2]Opening the lid during power failure・ 3-4 [3]Sterilization of rotor, buckets and
[3]Closing the lid・・・・・・・
・・・・・・
・・・・・ 3-5 tube rack ・
・・・・・・・・・・
・・・・・
・・・・・・4-4
3-4.Setting the Speed・・・・・・
・・・・・・
・・・・・ 3-6 4- 5.Greasing・・
・・・・・・・・・・・・・・・
・・・・・・4-5
[1] Setting the speed by the rpm・・
・・・・・ 3-6 4- 6.Inspection of Circuit Protector ・・・・・4-6
[2] Setting the speed by the centrifugal 4- 7.Using the Photoelectric Tach ometer Port
force( × g)・・・・・・・・・
・・・・・・
・・・・・ 3-7 ・・・・・・・・・
・・・・・
・・・・・・・・・・
・・・・・・4-7

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4- 8.Sp are Parts Supply・・・・
・・・・・・ ・・・・ 4- 8 6-6. RMP-23 Swinging Bucket Rotor・・・6-12
4- 9.Manufacturer requirements at Repair [1]Sp ecification ・・・・・・・・
・・・・・
・ ・・・・6-12
or Maintenance・・ ・・・・
・・・・ ・・・・・ ・ 4- 8 [2]Check the trunnion pin・
・・・・・・・・・・ 6-13
4-10.Product Preparation When Returning [3]Setting Buckets ・・・・・ ・
・・・・・・・・・・6-13
Units for Repair or fo r Other Reasons [4]Distribution of samples ・・・・・
・・・・・6-14
・・・・・・・・・
・・・・・・
・・・・・・・・・・
・・・・ 4- 9 [5]Setting plate・・・・・・・・・ ・・ ・・・
・・・・・6-14
Contaminant Elimin ation Certificate [6]Allowable weight of plate ・・・
・・・・・6-15
・・・・・・・・・・ ・・・ ・・・ ・・ ・・・・・・ ・ ・・・ 4-10 [7]Standard accessories ・・
・・・・・・・・・・ 6-15

Section 5. Troubleshooting
Section 7. Specifications
5-1.Alarm Indicators・ ・・・・・
・・・・ ・・
・・・・・ 5-1
E0・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-1 7-1.Centrifuge・
・・・・・・・・・・
・・・・・
・・・・・・7-1
C1 ・・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2 7-2.Standard Accessories ・・
・・・・・・・・・・・7-1
C2・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
C3 ・・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
C4・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
Section 8. Parts List
C5・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-2
5-2.Error Indicators・・・・・・・
・・・ ・・・
・・・・・ 5-3
8-1.Recommended Spare Parts・・・
・・・・・・8-1
E1・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-3
E2・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-3
E3・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-3
E4・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-4
E6・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-4
E7・ ・ ・・・・ ・・・・ ・・・・・・・・・・・・・
・・・・・ 5-4
5-3.Troubleshooting・ ・・・・・
・・・・ ・・
・・・・・ 5-5

Section 6. Rotor

6-1. Mounting RS-240 Rotor・・・・・・


・・・・6- 1
6-2. Mounting RS-1004 Rotor・・・・・
・・・・ 6- 2
6-3. Mounting RMP-23 Rotor・ ・・・ ・・・・・ 6- 3
6-4. RS-240 Swinging Bucket Rotor・・・・ 6- 4
[1]Specification ・・・・・・・・
・・・・・・
・・・・ 6- 4
[2]Setting Bu ckets ・・・・・・
・・・・ ・・
・・・・ 6- 6
[3]Tube distribution method・・・・・
・・・・ 6- 6
[4]Cushion s / Ad apter ・・・・・・・・・・・・・6- 7
6-5. RS-1004 Swinging Bucket Rotor・・・ 6-10
[1]Specification ・・・・・・・・
・・・・・・
・・・・ 6-10
[2]Setting Buckets ・・・・・・
・・・・・・
・・・・ 6-11
[3]Tube distribution method・・・・・
・・・・ 6-11
[4]Standard accessories ・・・・・・・・
・・・・6-11

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General Notes Be sure to follow them.
Since large electrical and mechanical energies are present on the centrifuge and rotor,
reasonable care is required for their handling.
Otherwise, failure may occur resulting in property damages or fatal physical injuries.
In order to prevent them from happening, be sure to follow the instruction given below.

WARNING
(1)Maximum load (5)While the centrifuge is in operation
Do not exceed the maximum load Whilethe centrifugeis in operation,
of rotor and bucket. do not stand closer than 30 cm to
The rotor or bucket used beyond avoid asecondary accident.
the allowable load level can be
damaged, thereby causing an ac-
cident. (6)Lid
Do not open the lid when the rotor
(2)Maximum speed is spinning.
Physical contact with the spinning
Keep the rotor and bucket speed rotor or bucket may cause serious
below the maximum speed. injury.
Excessive speed may cause dam-
age to the rotor, bucket and the (7)Sterilization
centrifuge. The maximum speed
depends on the rotor and bucket Do not perform dry-heat or auto-
strength. clave sterilization with tempera-
tures higher than those specified.
(3)Modification and unspecified parts
Otherwise, the rotor may deterio-
Do not modify, nor use unspeci- rate and cause an accident.
fied parts.
Unauthorized retrofit of the centri-
fuge, rotor or bucket, or use of un- (8)Rotor and drive shaft during rotation
authorized parts for them can re-
sult in accidents. Do not touch rotor and drive shaft
during the rotation.
Physical contact with the spinning
(4)Hazardous material rotor or drive shaft may cause
serious injury.
Do not put any hazardous materi-
al (explosive, chemically active,
organic, or radiation containing (9)Damaged, corroded, rusted or deformed
material, or material contaminated
by pathogenic microorganisms) as Discontinue use of the equipment
a sample of the centrifuge and do when its rotor or the buckets
not place it closer than 30 cm to found to have been damaged,
avoid a secondary accident corroded, rusted or deformed.
should the centrifuge accidentally Otherwise failure may occur.
rotate and contact the material.

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WARNING
Lifetime of rotors 3. Water pipes
City water pipes may not be
Use of rotors beyond the lifetime adequate as a ground since it
may lead to breakage of the rotor. may consist of plastic piping.
If the rotor is used continuously
even after the lifetime of the rotor Ensure that the ground cable is
has expired, should the rotor get connected to the grounding termi-
damaged an accident may occur. nal.
This precaution must be strictly
observed to avoid accidents due
Grounding to electric shocks or leakage.
Do not connect the ground cable
to the following places: Installation
1. Gas piping
Explosion or fire may occur. A clearance of 30 cm minimum
2. Ground cable of lightning must be provided around the cen-
Conductor, or telephone cable. trifuge.
Electric shock may occur in the If the centrifuge is driven into un-
case of lightening. controlled rotations due to a fail-
ure, secondary damage can result
from energy absorbed by the rota-
tion.

CAUTION
Installation
Do not install the centrifuge in a dusty
Do not install the centrifuge on an
place.
inclined, slippery, or unstable sur-
face. Do not install the centrifuge in a place
Violent vibration may occur. with poor ventilation.
Otherwise inside temperature of the cen-
Do not install the centrifuge in a trifuge may rise, resulting in accidents.
place where the temperature is
below 10 or over 40 . Do not install the centrifuge in a place
A place with the ambient tempera- with high humidity (relative humidity 85%
ture beyond 40 can introduce or above).
undesirable build-up of heat inside Leak or accident may occur.
the centrifuge and a place under
10 can cause the centrifuge
malfunction and, as the result, ac-
cidents.

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CAUTION
Toxic or radioactive substances etc. Balance of sample
When centrifuging of substan- Keep the load (of the sample,
ces contaminated with patho- bucket, etc.) balanced.
genic bacteria, or toxic or radio- If an appropriate balance is not
active substances, always use provided, unexpected accidents
containers that are pathogenic can result from a damaged rotor
bacteria, toxic substance or ra- or centrifuge.
diation proof.
Otherwise, infections, intoxica-
) Cushion
tion or radioactive exposure ac-
cidents may occur. Replace the cushion when the
glass or plastic tube is cracked.
Fasten a rotor If the cushion with glass frag-
Ensure that the rotor is firmly ment cut to it is used, the tube is
fastened to the drive shaft. easily cracked.
If not positively held in place,
the rotor or centrifuge can be Cleaning
damaged, thereby causing acci- Do not use detergents exceed-
dents.
ing the range of pH 5-8 or chlor-
ine detergents for washing pur-
Bucket poses.
The same type buckets must be Corrosion may damage the rotor
provided to every rotor yoke. and bucket resulting in damage
If not positively held in place, the to the centrifuge.
rotor or centrifuge can be dam-
aged, thereby causing accidents. Caution plate
Do not remove the caution
Tuberack plates.
Use the same type of tube racks. When a caution plate becomes
The wrong arrangement will dirty, blurred or peeled off, re-
cause imbalance and resulting in place it with a new one(caution
damage to rotor, bucket or the plates are available at charge).
centrifuge.

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Usable Rotor
WARNING
(1)Do not use any rotors other than those specified in (2) below.
If rotors other than those specified are used, the rotors may be broken, resulting in
a serious accident.
(2)The rotors that can mount as of May 2005 are as follows:
This information is subject to addition or change.
For information after June 2005, please contact your local dealer.

Swinging Bucket Rotor Plate Rotor

RS-240
RMP-23
RS-1004

Lifetime of rotors
WARNING
Use of rotors beyond the lifetime may lead to breakage of the rotor.
If the rotor is used continuously even after the lifetime of rotors has expired, should
the rotor get damaged, the main unit of the centrifuge suddenly may start to rotate;
this could result in an accident causing injury or death.

Lifetime of rotors is 7 years after the delivery.

When 7 years have passed after the delivery, discontinue operation of the centrifuge to replace the
rotor with a new one.
Earlier replacement, however, is required if any corrosion, lowered strength, flaw or deform due to
incorrect operation is detected on the rotor.
In such case, contact your local dealer and be sure to have the rotor checked before reusing it.

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Number of times allowed for autoclaving of rotor
WARNING
Stop the use of the rotor immediately when it is used beyond the number of
times allowed for autoclaving. Otherwise, the rotor may deteriorate by the heat
generated by autoclaving, resulting in deformation or destruction.
Should the rotor get damaged, the main unit of the centrifuge suddenly may
start to rotate; this could result in an accident causing injury or death.

The number of times allowed for autoclaving of each rotor should be deemed as follows.
When the following conditions has been met, discontinue operation of the centrifuge to
replace the rotor with a new part.
Earlier replacement, however, is required if any corrosion, lowered strength, flaw or deform
due to wrong operation is detected on the rotor. In such case, contact your local dealer and
be sure to have the rotor checked before reusing it.

[1] Number of times allowed for autoclaving and temperature of rotor

Number of times
Rotor Temperature of autoclave
allowed for autoclaving

RMP-23 121 ℃ 50 times

RS-1004 121 ℃ 100 times

[2] Recording autoclave


After each autoclave process, be sure to record the following (1)to (3)to control how
many times the autoclave is executed.
  (1)Date
(2)Temperature of autoclave
(3)Time of autoclave

You can take advantage of using the “ Autoclave record table ”attached to
the rotor instruction manual.

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Section 1 Component Name and Explanation
1 - 1.Appearance

Hook

Lid

Tachometer port
Refer to Page 4-7.

Chamber

Drive Shaft
Bucket intervention
prevention plate
This plate prevents the touch of
the bucket.
Do not remove this plate, when
you remove the rotor.

“OPEN”button
The lid opens when it is
pushed during the operation
stop with the power on.
Refer to Page 3-4.
S PE
ED

100 rp m

10 0 g T IM
E

mi n

ACC
sec

Front cover
D EC S TA R T
FU NCT IO N

1 2
M EMO R 3

Refer to Page 3-5.


Y

S TO P

Lid extreme release cap


Refer to Page 3-4.
Control panel “POWER"switch
Refer to Page 1-2.

1-1

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Section1 Component Name and Explanation

1-2.Control Panel

“SPEED/TIME"knob “TIME" display


(Setting the speed, RCF and time. )
Refer to Page 3-6.

“SPEED" display “START" key


(Speed / RCF ) When this key is pressed
the operation will be
started.
Refer to Page 3-2.
SP E E D

100 rp
m TIM E
100 g
m in

s ec
S TA R T
AC C
DE C F UN C T I
ON

1 2
3
MEMO
RY

S T OP

“ACC / DEC" key “STOP"key


(To change for ACC and DEC. ) Press this key to
Refer to Page 3-9. discontinue operation.
“FUNCTION" key Refer to Page 3-2.
(To change for speed /
RCF and time. )

MEMORY selection key


This key allow the selection
of saved programs.
Pressing the key will light
the lamp.
Refer to Page 3-10.

1-2

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Section 2 Installation and Power Supply
2-1.Unpacking
When the centrifuge is taken out of a corrugated carton box, check the following.
(1)Upon receiving the centrifuge, examine it for any visible damage caused during
transportation.
If any is found, contact the dealer immediately.
(2)Confirm that all the accessories listed in [7 - 2. Standard Accessories] are included with
the delivery. Refer to page 7-1.

2-2.Place of installation
WARNING
A clearance of 30 cm minimum must be provided around the centrifuge.
If the centrifuge is driven into uncontrolled rotations due to a failure, secondary
damage can result from energy absorbed by the rotation.

CAUTION
Do not install the centrifuge on an inclined, slippery, or unstable surface.
Violent vibration may occur.

When installing this centrifuge, keep 3meters or more away from AM radio.
Noise generated by this centrifuge causes the sound of AM radio deteriorated.

2-3.Movement of centrifuge
WARNING
● Never move the centrifuge while the rotor is rotating or while the rotor is
attached to the centrifuge. Otherwise, the drive shaft may become bent
or the rotor and the bucket may come off, resulting in an accident or
damage to the centrifuge.
● Ensure that rotor and bucket are removed from the centrifuge and that
the power cord is disconnected from the wall socket.
● Moving the position of the centrifuge while the power is turned on may
cause electrification accident or functional failure of the centrifuge.

Contd. on next page.


2-1

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Section 2 Installation and Power Supply

CAUTION
■ When moving the centrifuge, lift up the bottom of the centrifuge
body by two or more persons using equal forces to move the
centrifuge to the desired place.
■ Do not drop the centrifuge, otherwise damage or injury may occur.

2-4. Power Requirement


WARNING
Prepare a power supply that meets the following conditions.
1. The power supply voltage must be the same as that indicated on the
nameplate of the centrifuge and the voltage variation must be within
the range given in Table 2-1.
2. The current capacity must be more than given in Table 2-1.
3. In connecting the cord, install a knife switch or circuit breaker of given
value in Table 2-1.
When the centrifuge is connected to an outlet by means of a plug, use
a 3-pin plug with a ground terminal.
4. Use single-phase power.
5. The outlet must have a ground terminal and its ground resistance must
be less than 100 ohm.

Rated Voltage 100-115 V 200-240V

Acceptable Voltage Range 90V to 126V 180V to 264V

Current requirement 8A 5A

Table 2-1 Rated Voltage, Current and Acceptable Voltage Range and Current Requirement

WARNING
● The power cable alone should be connected to the plug socket.
● Do not use a branched plug socket, which may cause overheating or fire.

2-2

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Section 2 Installation and Power Supply

2-5. Grounding
Inlet
NOTE
When using the centrifuge for the
first time, plug the power cable on
the centrifuge into the inlet on the
back of the centrifuge. Power plug
 

WARNING
Ensure that the ground wire is connected to the grounding terminal.

WARNING
Do not connect the ground cable to the following places:
1. Gas piping
Explosion or fire may occur.
2. Ground cable of the lightning conductor, or telephone cable. Electric
shock may occur in case of thunderbolt.
3. Water pipes
City water pipes may not be adequate as a ground since it may be
connected to plastic pipework.

Knife switch water pipes Gas piping

Ground terminal
Plug Socket
Power Power
(Black) Power plug
(W hite)

Ground wire
(Green-and-yell ow stripe Ground terminal
pattern)

2-3

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Section 3 Operation
3-1.Cautions of Operation

When using this centrifuge, observe the contents of the Section "General
Notes" being described in the front part of this document and the
precautions given in respective sections.

3-2.Operation 

Operation 1. Turn on the “POWER" switch .

Operation 2. After the “STOP”lamp on the control panel lights, press the “OPEN”button.

Operation 3. Mount the rotor on the drive shaft. NOTE


Refer to Section 6. Install the rotor after checking if the
"Bucket intervention prevention plate" is
installed on the drive shaft.
Refer to page 1-1.

Operation 4. For swinging bucket rotor ,mount buckets on the rotor.

Operation 5. Place the sample in the rotor or buckets.


Refer to Section 6.
NOTE
Operation 6. Close the lid firmly. If the lid is not closed properly the
centrifuge is not able to start.
Make sure the lid is closed firmly.

Operation 7. Proceed to operation 9 when operating with the same setting values as before.

Operation 8. Set the respective parameters.


・Set to required speed. Refer to page 3-6.
・Set to required time. Refer to page 3-8.
・Set braking force during deceleration and acceleration speed with the“ ACC/DEC”
key Refer to page 3-12.
● When the memory has been saved, press the selection key.
Refer to page 3-10.

NOTE
Press the "SPEED/TIME" knob to check the "centrifugal force" of the set
speed or "speed" of the set centrifugal force.

Contd. on next page.


3-1

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Section 3 Operatio n

Operation 9. Press the “START"key.


“ Pip"sound and lamp on the“START" key lights.

START

min Value on the “TIME” display starts decrea-


sec sing.

min
When the value becomes 0 , the centrifuge
sec automatically decelerates and stops.

Operation 10.

*** In the following case ***

(1)When you want to change the setting (speed, RCF, time) during the operation.
Press the “FUNCTION" key 1 or 2 times on the control panel after changing the
setting, and the flashing of the display is stopped.
The operation becomes shifted to the setting that have
FUNCTION
been changed.

(2)When you want to halt operation, Press the“STOP" key


on the control panel.
STOP

There is the sound and the rotor will come to a halt.

In case that the operation is stopped before the end of the period set by the
timer and is resumed, the operation period after resumption is the remained
period of the operation.
If the knob is pressed before resumption of operation, the operation period
returns to the originally set period.

3-2

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Section 3 Operation

When the rotor has stopped, the “STOP" lamp flashes and an sound that informs the end of
the operation is issued.
● The “ Sound that informs the end of the operation ” can be selected from the 5 kinds +
Sound none. Refer to page 3-14.
● The “ Alarm Indicators ” to show the end of the operation can be selected from the 5
kinds. Refer to page 3-17.

Operation 11. Press the“OPEN" button to open the lid.


“STOP”lamp remains lit.

Operation 12. Remove the sample.


Refer to page 3-16.

Operation 13. If the centrifuge is to be used again, return to Operation 5. 

Operation 14. After finishing use of the centrifuge, turn "OFF" the "POWER" switch to turn off
the power supply, leaving the lid in opened state.

NOTE
If the power is turned off while a memory being called up and is
then turned on again, the same memory as displayed before the
power cut will be called up and displayed.
Ex. : Turn off the power while“MEMORY 2”called up. Turn on the power,
the“ MEMORY 2”key lamp will light and the parameter stored under
“MEMORY 2”key will be displayed.

3-3

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Section 3 Operation  

3-3.Opening and Closing the lid


[1]Turning on the power and opening the lid

(1)Turn on the the “POWER" switch to turn the power on.


The “POWER" switch locates on the front side panel of
the centrifuge.

(2)The〈STOP 〉lamp on the control panel will be lit.

(3)Pressing the〈OPEN〉button the lid can be opened.

NOTE
Open
The lid is locked in following
cases and will not open even if
the〈OPEN〉button is pressed.
・ When the rotor is spinning.
“OPEN ”button ・ When there is a power failure
S
P
EED

00r pm
1
100g T
IM E
or the power is turned off.
mi n
ACC s
DEC ec START
FNCTION
U

1 3
M2
MEOY
R
ST
OP

[2]Opening the lid during power failure

WARNING
Do not open the lid while the rotor is spinning.
When a power failure occurs during operation of the centrifuge, the rotor
will naturally decelerate to stop its rotation. If the lid is unlocked forcibly
before the rotor stops, the person can be entangled in the rotor, resulting
in a serious injury or death.

(1)Turn off the “POWER”switch.

(2)By using a flat-blade screwdriver, remove the lid


extreme release cap on the lower part of the left
side of the unit. TIME AR
STT

While pulling the string straight, push “OPEN”


EE
SPD
OP
ST
IO
NC
FUTN

button. (Do not pull the string too strong.)


Then the lid opens. String

3-4

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Section 3 Operation

◆ If the lid extreme release cap string is cut

WARNING
For prevention of electric shock, wait at least 5 minutes before opening the
front lid.

(1)Turn off the “POWER" switch.

(2)Remove the power supply plug from the outlet.

(3)When more than 5 minutes have passed after turning off the power, remove the 2
screws on the left and the right sides of the centrifuge.

(4)Insert a minus driver into the clearance between the front cover and the centrifuge, and
turn the front cover over gently to the front side.

Minus driver

Space

Screws EED
SP
1 pm

(two)
00r
100g TI ME
mi n
AC
C c
se
DEC STA
RT
FUNIO
CTN
1
O23
MEMRY
SP
O
T

(5)Press the “OPEN”button while holding down the lock plate.


The lid opens after the lid lock has been released.

Lock plate
Push
Push
“OPEN”button

String Connection stick


(When the string is cut, it can be fixed by
The inside of the centrifuge tying the ends.)

[3]Closing the lid


Close the lid by pressing the front center of the lid by hands.
Press the lid securely and lock it.

NOTE
The lid can be closed even
when there is a power failure
or the power is turned off.

3-5

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Section 3 Operatio n

3-4.Setting the Speed 


[1]Setting the speed by the rpm
(1)Press the “FUNCTION”key.
The “SPEED”display flashes and indicates the current setting.
SPEED
×100rpm
FUNCTION
×100 g

(2)Check if the〈× 100 g〉lamp turned off.


① When the〈× 100 g 〉lamp is off ・・
・・・・ ・
・・・・・
・・・
・・ Proceed to the Procedure(3).
SPEED
×100rpm

×100 g

② When the〈× 100 g 〉lamp is on・・


・・・・
・・・
・・・
・・・
・・・ Press the “ SPEED/TIME ”
SPEED knob and the〈× 100 g〉lamp
×100rpm turns off.
Proceed to the Procedure(3).
×100 g

(3)Turn the “SPEED/TIME”knob to set to required speed.


The speed can be set 100 rpm intervals.

SPEED
×100rpm

×100 g

(4)When continuing on to time setting, press the “FUNCTION”key.


The “SPEED”display stops flashing and the “TIME”display starts to flash.
(Timer setting status)
SPEED TIME
×100rpm min
FUNCTION
×100 g sec

NOTE
● The centrifuge can be started with the speed on the "SPEED" display,
even if the display of value is flashing.
● When you want to change the setting during the operation, press the
“ FUNCTION" key 2 times on the control panel after changing the
setting, and the flashing of the display is stopped.
The operation becomes shifted to the setting that have been changed.
● The setting order of the speed and the timer can be changed.
Refer to page 3-15.

3-6

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Section 3 Operation

[2]Setting the speed by the centrifugal force ( × g)

(1)Press the “FUNCTION”key.


The “SPEED”display flashes and indicates the current setting.
SPEED
×100rpm
FUNCTION
×100 g

(2)Check if the〈× 100g 〉lamp turned on.


① When the〈× 100g 〉lamp is on ・ ・・・ ・
・・・・
・・・・
・Proceed to the Procedure (3).
SPEED
×100rpm

×100 g

② When the〈× 100g 〉lamp is off ・


・・・
・・・
・・・・
・・・Press the “SPEED/TIME”knob
SPEED and the 〈× 100g 〉 lamp turns
×100rpm on.
Proceed to the Procedure (3).
×100 g

(3)Turn the “SPEED/TIME”knob to set to required centrifugal force reading.


Indications on the “SPEED”display are at 100 × g intervals.

SPEED
×100rpm

×100 g

(4)When continuing on to time setting, press the “FUNCTION”key.


The “SPEED”display stops flashing and the “TIME”display starts to flash.
(Timer setting status)

SPEED TIME

×100rpm min
FUNCTION
×100 g sec

NOTE

● The centrifuge can be started with the speed on the "SPEED" display,
even if the display of value is flashing.
● When you want to change the setting during the operation, press the
“ FUNCTION" key 2 times on the control panel after changing the
setting, and the flashing of the display is stopped.
The operation becomes shifted to the setting that have been changed.
● The setting order of the speed and the timer can be changed.
Refer to page 3-15.

3-7

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Section 3 Operatio n

3-5.Setting the Timer


(1)Pressing the "FUNCTION" key 2 times, the “TIME ”display flashes and indicates the
current setting.
The timer is able to be set up.

min
FUNCTION
sec

(2)Pressing “SPEED/TIME" knob changes the settable range of centrifuging time as des-
cribed below. Set to the range as required.
Factory default,the range is set minutes.
① Setting minutes

Settable range 1-99 minutes min

Setting unit 1 minute sec

② Setting seconds
min
Settable range 1-99 seconds
sec
Setting unit 1 second

③ HOLD min
It will continually operate regardless of
sec
the timer. When set to hold, both "min"
and "sec" lamp will be turn off.

(3)Turning the “SPEED/TIME" knob changes


the indication on the display. min
Set to the time required.
sec
(4)Press “START" key on the control panel.
The operation will be started.
When started, the display will diminish and stop at point 0.

NOTE
● In case that the operation is stopped before the end of the period set by the
timer and is resumed, the operation period after resumption is the remained
period of the operation.
If the "SPEED/TIME" knob is pressed before resumption of operation, the
operation period returns to the originally set period.
● The centrifuge can be started at the time when the setting value is flashing at
"TIME" display.
● When you want to change the setting during the operation, press the
“ FUNCTION" key on the control panel after changing the setting, and the
flashing of the display is stopped.
The operation becomes shifted to the setting that have been changed.
● The speed and the timer can be set in this sequence or vice versa.
As for the change of the setting, please refer to page 3-15.

3-8

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Section 3 Operation

3-6.Setting the acceleration・deceleration


When blowing-up of samples is feared, adjust the acceleration and the
deceleration setting by pressing the “ACC/DEC”key

SL OW
RAPID acceleration
acceleration It changes to the SLOW characteristic
[Ex.] from RAPID at 1000rpm.
SPEED

1000rpm

TIME
Natural dece lerati on

RAPID SLOW
deceleration deceleration
NOTE
You can set the natural deceleration starting speed N.
Refer to page 3-13.

ACC ACC:RAPID(“ RAPID ” acceleration )


DEC DEC:RAPID (“ RAPID ” deceleration)

ACC ACC :RAPID (“ RAPID ” acceleration )


DEC DEC :SLOW (“ SLOW ” deceleration)
On

Off ACC ACC:SLOW(“ SLOW ” acceleration )


DEC DEC:RAPID(“ RAPID ” deceleration)

ACC
ACC:SLOW (“ SLOW ” acceleration)
DEC
DEC:SLOW (“ SLOW ” deceleration )

3-9

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Section 3 Operatio n

3-7.Saving the Memory


Saving the setting value enables operation with the same setting
value repeatedly.

[1]Saving the Memory


(1)Set the respective parameters.
Refer to the following page for the setting method.
[3-4.Setting the speed ] Refer to Page 3-6.
[3-5.Setting the timer] Refer to Page 3-8.
[3-6.Setting the acceleration ・ deceleration ] Refer to Page 3-9.

(2)Pressing “FUNCTION”key, the setting is settled.


1 2 3
(3)Pressing "MEMORY" key, the lamp is lit, and choose MEMORY
the lamp number you want to save.

(4)Keep pressing “FUNCTION”key until the buzzer FUNCTION About 3 seconds


sounds. (About 3 seconds)

NOTE
The parameters necessary for operation (speed, RCF, time, acceleration /
deceleration, etc.) can be stored as programs with MEMORY 1~3 keys.

(5)Stored memories can be easily recalled by pressing the


1 2 3
appropriate memory selection key. MEMORY

NOTE
Even if the power is turned off, the parameters set at the time of power off
remain stored.
The last setting is displayed and can be operated.

[2]Memory cancellation

Pressing the “Function ”key or the “ACC/DEC”key, the normal setting mode
will be selected.

① Pressing the “Function ”key. FUNCTION


The change for speed / centrifugal force or time.

② Pressing the “ACC/DEC”key. ACC


The change for ACC and DEC. DEC

3-10

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Section 3 Operation

3-8.Setting the Function


As for this product the following setting is produced.
Choose the item you want to set up, and press "SPEED/TIME" knob.
The page where the content is written is referred to.
NOTE
The memory lamp turns off temporarily, when you set the function while
setting the memory operation in Model 2420. The lamp is lighted after the
setting.

FUNCTION
(1)Pressing the "SPEED/TIME" knob while holding
down the "FUNCTION" key .

SPEED TIME
(2)The used number of times of the ×100rpm min
centrifuge is displayed. ×100 g sec
[ Ex.]186 times

(3)Setting the Rotation Radius SPEED TIME


×100rpm min
Refer to Page 3-12.
[Ex.]146 mm ×100 g sec

SPEED TIME
(4)Setting the "SLOW" deceleration. ×100rpm min
Refer to Page 3-13.
×100 g sec
[Ex.]300rpm

SPEED TIME
(5)Setting the Sound that informs the
×100rpm min
end of the operation.
Refer to Page 3-14. ×100 g sec
[Ex.]1
SPEED TIME
(6)Reverse of setting order of the ×100rpm min
Speed and the Timer. ×100 g sec
Refer to Page 3-15.
[Ex.]0
SPEED TIME
×100rpm min
(7)Setting the Reminder alarm.
Refer to Page 3-16. ×100 g sec
[Ex.]ON
SPEED TIME
(8)Setting the Indicators for the end of ×100rpm min
the operation. ×100 g sec
Refer to Page 3-17.
[Ex.]1

■ After setting any one of the above, please return the display to the previous indication of
"SPEED" and "TIME".
To do above, press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
3-11

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Section 3 Operatio n

[1]Setting the Rotation Radius


To obtain more accurate centrifugal force, adjust the rotation radius to that of the rotor
buckets.
Factory default : 146 mm .

(1)Pressing the “SPEED/TIME”knob while holding FUNCTION


down the “FUNCTION ”key .
The used number of times of the centrifuge is displayed.

(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the left side of
the “SPEED”display.
The “SPEED”and “TIME”display indicates the current setting.
Unit : mm
Refer to Page 3-11.
[Ex.]The rotation radius is 146 mm.

SPEED TIME
×100rpm min

×100 g sec

3 digits 2 digits 1 digit

(3)Turn the “ SPEED/TIME”knob to set to the rotation radius of the rotor bucket to be
used for operation.
The range is set from 50 to 150 mm.

SPEED TIME
×100rpm mi n

×100 g sec

3 digits 2 digits 1 digit

(4)Press the “SPEED/TIME”knob while holding down the “FUNCTION" key.


The display returns to the previous Speed and Time indication after the setting (rotation
radius) is saved.

FUNCTION

NOTE
The rotation radius can not be stored in the memory, when the power
supply is turned off without returning the display to the previous
indication.

3-12

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Section 3 Operation

[2]Setting the "SLOW" deceleration


The characteristics have become as shown in the graph.
You can set the natural deceleration starting speed N by the following method.
Factory default,it is set as 0 rpm.
The setting range of "N" is from 0 to 4,000 rpm.

(A)N = 1,000 rpm or more (B)N = 1,000 rpm or less

speed speed RAPID


rpm RAPID
rpm SLOW
N It changes to the SLOW
characteristic from RAPID
Natural at 1000rpm.
decelerati on 1000
1000 Natural
decelerati on

TIME TIME

(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.

(2)Pressing the "SPEED/TIME" knob several times and let indicate " "on the "TIME"
display.
The "SPEED" display indicates the current setting of the natural deceleration speed.
Unit : rpm
Refer to Page 3-11.
[Ex.] Setting is the 0 rpm.
SPEED TIME
×100rpm min

×100 g sec

(3)Turn the "SPEED/TIME" knob to set the natural deceleration speed.


The setting range of "N" is from 0 to 4,000 rpm.
[ Ex. ] Setting the 300 rpm.
SPEED TIME
×100rpm min

×100 g sec

(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting ( Natural
deceleration speed) is saved.

FUNCTION

3-13

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Section 3 Operatio n

[3]Setting the Sound that informs the end of the operation

The “ Sound that informs the end of the operation ” can be selected from the 5
kinds + Sound none.
Use this function to identify each “ Sound that informs the end of the operation ” when
you use more than one centrifuges.
Factory default : [ 1 ]

(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.

(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display. The "SPEED" display indicates the current setting of the sound that informs.
Refer to Page 3-11.
[Ex. ] The “ Sound that informs the end of the operation ” 1.

SPEED TIME
×100rpm min
×100 g sec

(3)Turn the "SPEED/TIME" knob to change the "Sound that informs the end of the opera-
tion".
Pressing the "FUNCTION" key makes the selected buzzer sound once.
The "Sound that informs the end of the operation" can be selected from 1, 2,3, 4,
5 of 5 kinds + 0 (Sound none).

SPEED TIME
×100rpm min

×100 g sec

(4) Then, press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting ( Sound
that informs the end of the operation) is saved.

FUNCTION

3-14

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Section 3 Operation

[4]Reverse of setting order of the Speed and the Timer


The setting order of the speed and the timer can be reversed.
This function is useful when you often operate the centrifuge by changing the
timer only.
Factory default : [ 0 ]

(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.

(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display. The "SPEED" display indicates the current setting of the number (see the figure
below). Refer to Page 3-11.
[Ex.]Numbers 0

SPEED TIME
×100rpm mi n

×100 g sec

Numbers Contents

0 「Speed / RCF」→「Timer」

1 「Timer 」→「Speed / RCF」

(3) Turn the "SPEED/TIME" knob to change the number.

SPEED TIME
×100rpm min

×100 g sec

(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting (Numbers)
is saved.

FUNCTION

3-15

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Section 3 Operatio n

[5]Setting the Reminder alarm


The alarm reminds the user every minute when samples are left inside the
centrifuge after completion of the operation.
The reminder alarm goes off when opening the lid or performing some opera-
tions.
Factory default : [ ON ]

(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.

(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display.The "SPEED" display indicates the current setting of the reminder on or off.
(see the figure below)
Refer to Page 3-11.
[Ex.]Reminder alarm : ON

SPEED TIME
×100rpm min
×100 g sec

Reminder alarm “SPEED”display.

(3)Turn the "SPEED/TIME" knob to change the number.


[Ex.]Reminder alarm : OFF

SPEED TIME
×100rpm min
×100 g sec

(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting (reminder
on or off) is saved.

FUNCTION

3-16

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Section 3 Operation

[6]Setting the Indicators for the end of the operation


Completion of the operation is indicated not only with an alarm but also with the
"SPEED" and "TIME" displays.
The "Alarm Indicators" can be selected from the 5 kinds.
Factory default : [ 1 ] (see the figure below).

(1)Pressing the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The used number of times of the centrifuge is displayed.

(2)Pressing the "SPEED/TIME" knob several times and let indicate " " on the "TIME"
display. The "SPEED" display indicates the current setting of the number (see the figure
below). Refer to Page 3-11.
[Ex.]Number : 1

SPEED TIME
×100rpm min

×100 g sec

Numbers “SPEED”and “TIME”display

0 After [Setting value]lights 、[0]lights

1 After [Setting value]flashing、[0]flashing

2 [88][88]flashing

3 [--][--]flashing

4 [E][nd]flashing

(3)Turn the "SPEED/TIME" knob to change the number.

SPEED TIME
×100rpm min

×100 g sec

(4)Press the "SPEED/TIME" knob while holding down the "FUNCTION" key.
The display returns to the previous Speed and Time indication after the setting (Numbers)
is saved.

FUNCTION

3-17

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Section 3 Operatio n

3-9. Calculating Centrifugal Force


You can readily calculate the centrifugal force by assigning the Rotation Radius and speed
in the following equation:

[formula 3-1] Spe ed N( rpm ) 2


Centrifugal force RCF( × g)= 11.18 × × Rotation Radius R(c m)
1000

Please refer to the Nomograph for Centrifugal Force Calculation at the last page of this
manual.

NOTE
As for rotation radius, please refer the page 6-4, 6-5, 6-10 and 6-12. The
maximum centrifugal force being indicated in the said pages is calculate by
substituting the maximum radius of gyration of the rotor for the radius of
gyration of each rotor (cm) and by rounding the first digit above the decimal
point of the calculation result.
By setting the rotation radius, an accurate speed and centrifugal force can be
switched. Factory default at the time of delivery is 14.6 cm.
Refer to page 3-12.

3-10.Allowable load and Reduced maximum speed

WARNING
Do not exceed the maximum speed and the allowable load of rotor and
bucket.
An excessive speed or overload may cause a damage to the rotor, bucket
and the centrifuge.
If the load exceeds the limit, reduce the maximum speed as shown in the
formula 3-2 and set the actual speed below the reduced maximum speed.

[ formula 3-2 ]
Allowable load (gram)
Reduced maximum speed(rpm )= Maximum speed(rpm )×
Actual load (gram)

(1)When the gravity of sample is 1.2 or higher or when a special tube is used,
check the allowable load of the rotor and bucket.
(2)Regarding allowable load on rotors and buckets at the maximum speed refer to the
appropriate Specification Table for rotor in use.
(3)The allowable load includes the weight of sample, cushions, adaptors, tubes, caps,
and racks. (The weight of bucket is not included in the allowable load.)

3-18

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Service
Section 4
4-1.Daily Inspection
WARNING
If any abnormality is found during routine daily inspections, discontinue
centrifuge operation, use the "POWER" switch to turn the machine OFF,
disconnect the power cable, attach "DO NOT USE" labels to the rotor and the
outside of the centrifuge, and contact your nearest dealer to request a centrifuge
inspection before resuming operation of the equipment.
If the centrifuge is used after discovering any abnormality, an accident could
occur, possibly leading to serious damage or accidents involving physical injury.

Checkpoint Action taken


Check whether the rotor knob Retighten, if either of these is loose. For details, refer
and rotor locking nut are to "Mounting the Rotor" in the rotor instruction
properly tight. manual. Refer to Section 6.
If the buckets do not move smoothly and freely, clean
To inspect, mount buckets on the bucket grooves and trunnion pins of the rotor yoke
the swinging bucket rotor using alcohol. Contact your local dealer and make an
when it is stopped, and lift up appointment for an authorized inspection if these
the buckets manually. parts fail to operate smoothly even after lubricant has
been applied. Refer to Page 4-5.
Check carefully for any
Do not use the rotor or buckets if any cracks,
cracks, evidence of corrosion, corrosion, rust or deformations are found. Contact
rust or deformation on the
rotor and buckets. your local dealer for an inspection.

Check that no foreign material


or water is present in the Remove any foreign material or water before
operating the centrifuge.
chamber.

If the lid lock does not work, discontinue operation of


Check that the lid is hook. the centrifuge. Contact your nearest dealer for a
centrifuge inspection.

If the screws fixing the lid hinge are loose or have


been removed, the lid may come off when the buckets
are thrown off and the broken pieces may cause injury
Check that the screws fixing
the lid hinges are not loose. to the persons in the vicinity of the centrifuge. If the
lid hinge is not fixed firmly, stop centrifuge
operation. Stop operation promptly and contact yo ur
local dealer for inspection.

Check the nut of the trunnion


If the trunnion pin is loosened, contact your local
pin (RMP-23 ) is not dealer for inspection.
loosened.
Check that the grounding wire
is correctly connected. Refer to Page 2-3.

Check that all knobs, If any do not operate correctly, contact your local
displays, lamps and switches dealer for an inspection.
operate correctly. Refer to Page 1-1 and 1-2.
Check that the centrifuge is
placed horizontally. Refer to page 2-1.

4-1

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Section 4 Service

4-2.Monthly Inspections
[1] Inspect the rotor

WARNING
Perform a monthly careful inspection of the rotor appearance. Check for
any deformation or damage, including the interior and bottom of the holes.
If any abnormality whatsoever is found, there may be significant damage or
corrosion of the rotor that could lead to serious damage or accidents
involving physical injury. Stop operation at once, put "DO NOT USE" labels
on the rotor and centrifuge lid, and contact your local dealer to arrange for
authorized service.

[2]Clean the rotor and buckets


Remove the rotor from the shaft, and clean it.
Refer to Page 4-3.

[3]Clean the chamber interior


Refer to Page 4-3.

4-3.
  Annual Inspection
Annual Inspection (fee required)
  For the following inspection items, please call your local dealer and set up an
appointment for a periodical inspection.

Motor Speed control


Rotor and bucket Imbalance
lid Electric current
Chamber Insulation
Speed meter Install the centrifuge
Timer

4-2

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Section 4 Service

4-4.Cleaning and Sterilization


WARNING
Consult with your nearest dealer prior to attempting any cleaning procedure for
the rotor, buckets, or tube rack that is not specifically recommended in this
manual. Certain cleaning methods or cleaning agents may cause corrosion and
then breakage leading to serious damage or accidents involving physical injury.

[1]Cleaning the chamber interior


The chamber is made of stainless steel (SUS304) but may rust if sample spillage
containing salt (NaCl) or chlorinated chemicals (Cl) is allowed to remain.
After using the centrifuge, follow the method below (1) when wiping the inside of
the chamber and cleaning the surfaces.

(1) Cleaning and rust prevention

Cleaning the chamber interior according to the following procedure will help
prevent rust.
1) Wipe off any dirt or residue using a cloth moistened with a small quantity of a neutral
detergent.
2) Wipe away any detergent residue using a cloth dampened with water, and then dry the
inside of the chamber.
3) To prevent rust, we recommend the use of common anti-rust agents (such as CRC-556)
only if such will not affect the samples being centrifuged.
Wipe the dirt off using a cloth containing a small quantity of neutral detergent.

(2) If rust is present

Superficial rust can be removed by using a mild solution of sodium bicarbonate


(NaHCO3, bicarbonate of soda, baking soda).
1) Moisten a paper towel with a little water, apply some bicarbonate of soda, and then rub the
area where rusting has occurred.
2) After removing the rust with the bicarbonate of soda, follow the above procedure (1) and
clean the chamber interior using a neutral detergent followed by a thorough wiping with
plain water. Make sure that such cleaning is especially thorough, so that all traces of the
bicarbonate of soda are removed.

[NOTE ]

Under no circumstances should any rust inside the chamber be removed by using
sandpaper or abrasive agents, since if the interior surfaces are scratched, this will
only increase the likelihood of additional rust developing.

Contd. on next page.


4-3

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Section 4 Service

[2]Cleaning the rotor, buckets and tube rack

CAUTION
Do not use detergents exceeding a pH range of 5 - 8, or chlorinated
detergents normally used for washing.
Corrosion may damage the rotor, bucket, or tube rack, resulting in damage
to the centrifuge that may lead to serious additional damage or accidents
involving physical injury.

  NOTE
Do not allow any spilt samples to remain on any surfaces, otherwise rust
or corrosion may occur. Also, if sample spillage is left between the rotor
and shaft, later detachment of the rotor may become problematic.

  1) If sample spillage has occurred, remove the rotor, buckets, and tube rack from the
centrifuge, and wash the affected items with a neutral detergent and warm water.
Then rinse the items with the distilled water and thoroughly dry them before use.

2) If water has accumulated inside the rotor, place the rotor with its bottom side up and allow
it to dry completely.

3) If a sample has spilled onto the drive shaft, wipe it off using a cloth moistened with a
small amount of a neutral detergent and then clean away all detergent traces using a cloth
moistened with water. Then, dry the surfaces completely before using the machine.

[3]Sterilization of rotor, buckets and tube rack

 
WARNING
Do not heat the rotor, buckets, or tube rack above 100 ℃ for sterilization
or disinfection purposes.
Also, do not use an autoclave for dry heat sterilization, since excessive
heat may deleteriously affect the strength of the rotor, buckets, or tube
rack, resulting in breakage of the rotor, buckets, or tube rack, causing
serious damage or accidents involving physical injury.
Yet, You can conduct up to 50 times of autoclave sterilizations of the
RMP-23 rotor and bucket at 121 ℃ and 100 times of autoclave sterilizations
of the RS-1004 rotor and bucket at 121 ℃ .

To disinfect the rotor, buckets, or tube rack, a 70 % ethanol solution, or ultraviolet


radiation, is recommended.
4-4

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Section 4 Service

4-5.Greasing

CAUTION
Be careful to maintain lubrication by ensuring that a proper amount of grease is
present in all bucket grooves (section "A" in the schematic diagram below). A
lack of grease may allow violent vibration to occur, resulting in damage to the
centrifuge.

NOTE

1) If the buckets do not swing up smoothly as the rotor is spun up to


speed, apply the attached grease to the section A shown in sketch
below. The buckets should then swing up smoothly.
2) Wipe off any dirt or congealed material before applying fresh grease,
using alcohol or acetone.
3) If the buckets do not swing up properly after applying the grease,
contact your local dealer for an inspection.
4) When the provided grease is used up, purchase replacement grease of
the following code number from your nearest local dealer.

Rotor Grease code No.

RMP-23
S70284
RS-1004

Apply the attached


grease

Trunnion pin

Rotor yoke

4-5

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Section 4 Service

4-6.Inspection of Circuit Protector


The circuit protector is automatically activated when the electric circuit failure or
overcurrent occur.
In such a case, the white portion of the circuit protector, located at the bottom left on the
rear side of the centrifuge, will stick out.

Circuit protector

Recovery procedure is as follows :

(1)Turn off the power switch.

(2)After the rotor stops completely, open the lid and check the rotor, following
the procedure given in [3 - 3. Opening and Closing the lid 2. Opening the lid during
power failure] on Page 3 - 4.

(3)Close the lid , and then push the sticking out white portion until it becomes unseen.

(4)Turn on the power switch.

(5)Re - start operation and see if it works.

If the circuit protector is activated again, contact your local dealer.

4-6

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Section 4 Service

4-7.Using the Photoelectric Tachometer Port


A tachometer port (transparent window) is provided at the top of the lid.
Through the tachometer port, the actual rate of rotation can be measured,
using a photoelectric tachometer.
(1)When using a photoelectric tachometer, place a piece of reflective tape 5 - 20mm away
from the center of the rotor.
20mm
5mm

Reflective tape

(2)Mount the rotor on the drive shaft.


Refer to Section 6.

(3)After turning the power ON, set the photoelectric tachometer so that its tip is right above
the tachometer port on the lid. The photoelectric tachometer can then measure the actual
rotation rate.

Photoelectric tachometer

Tachometer port
Lid

Centrifuge

Do not apply force to the tachometer port window from inside the lid.

4-7

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Section 4 Service

4-8. Spare Parts Supply


NOTE
Spare parts (parts necessary to the maintenance of equipment functions)
will be available for seven (7) years after production of the particular
product is discontinued. We therefore beg your indulgence and
recommend that you be prepared for some situations in which certain
repairs are impossible due to a lack of spare parts.

4-9. Manufacturer requirements at


Repair or Maintenance
We cannot repair or inspect a centrifuge if it has not been properly
washed or sterilized beforehand, or if it has been used in an RI facility or a
facility operating at a level higher than P2. Please ensure that each
machine has been properly washed and sterilized before offering it for
repair or inspection.

[1]Required information when submitting an inquiry

Necessary information Note


1. Model 2420
2. Serial number No.
See the name label at the left side panel of centrifuge.

3. Trouble Provide a description of the trouble as detailed as


possible.

[2]Required information that must be attached to the centrifuge in the


form of a note

(1)If you have pertinent information on your handling of biohazards, fill out the
items below, and affix this information to the centrifuge.

1. Environment where the centrifuge is used.

2. Sample
3. Required measures against the biohazard.

(2)Please attach a "Contaminant Elimination Certificate" to the centrifuge.


Refer to Page 4-9 and 4-10.
4-8

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Section 4 Service

4-10. Product Preparation When Returning


Units for Repair or for Other Reasons
[1]Remove any contaminants from units or items that will be returned for
servicing

Prior to sending back our products (including accessories) for repair or for other
reasons, be sure to remove any and all contaminants.

[2]Attach a Contaminant Elimination Certificate


To eliminate possibly harmful contaminants, the user must take appropriate action on
his or her initiative. Fill out a "Contaminant Elimination Certificate" as shown on page
4-11, and attach it to the item to be returned.
[Caution]
"Contaminant" means radioactive materials, poisonous materials, contagious pathogens,
and so on. The preparatory procedure to eliminate all traces of such contaminants
therefore varies depending on the respective materials. Please take appropriate action to
prevent any possible hazard to personnel who will be handing the machine, equipment
or parts.

[3]Equipment contamination elimination goal

The elimination of contaminants from the equipment is aimed to safeguard the persons
who will inspect and repair the returned products at our facilities.

[4]If a Contaminant Elimination Certificate is not attached:

If a "Contaminant Elimination Certificate" is not attached to the returned products we


receive, we will contact the person in charge to confirm the details.

If we cannot confirm that potentially hazardous contaminants have been properly


eliminated, and we find that we cannot adequately deal with such matters ourselves, we
may return the products to the customer without taking further action.

[5]Contaminant elimination fee


If we are forced to perform the work of eliminating contaminants, we may request that
you pay a fee. Please note this stipulation beforehand.

4-9

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Please make a copy of this page and fill out the relevant details.

Contaminant Elimination Certificate


Date :    ,    ,   

Name:             
Company / Institute:

                                   
Department:

                                   

Tel:                Fax:             

Address:                            


We hereby certify that contaminant elimination for this equipment has been
performed as follows:
Type of unit:             Serial No.            
Type of unit:             Serial No.             

Accessories :                              
Date when contamination elimination was carried out.

Date:      ,     ,    

Contaminants:
                                   

Method used:
                                   
                                   
                                   

Dealer notification:
                                   
                                   
                                   

Signature:

              

4-10

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Section 5 Troubleshooting
5-1.Alarm Indicators
(1)When trouble occurs during operation, the “ TIME" display flashes to indicate the alarm.

(2)When any of the alarms become activated during ope ration (whe n the rotor is spin-
ning), the rotor immediately dece lerates and stops.

[Ex.]
Control panel “TIME"display
TIME
min

sec

NOTE
The equipment is inoperative while any of the alarm indicators is turned on.

Alarm Checkpoint Action taken


Indicators

Setting speed alarm


An error occurs immediately after
the "START" key is pressed when Pressing the “ START ” key
E0 the speed is more than 4,000 rpm makes the centrifuge auto-correct
(this may happen when centrifugal the speed to 4,000 rpm.
force after changing rotation
radius is changed to speed).

Cont’
d. on next page.

5-1

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Section 5 Troubleshooting

Alarm Checkpoint Action taken


Indicators

C1 Time alarm for rotor inspection


Because the inspection time has
C2 arrived, the alarm is displayed.
The inspection time for the rotor has
arrived.
C3 1-5 represents the level of the number
Contact your nearest dealer for
of times of rotor use.
C4 As for the details, please refer to the
inspection of the rotor and bucket.

C5 following.

"Alarm Indicators" and "Number of times of rotor use" at the time of rotor inspection

Each "Alarm Indicator" is displayed at the time when the number of times of rotor use reaches
the figures shown below.

Alarm Number of times of rotor use at


Indicators the time of the alarm indicators

C1 50,000~50,050

C2 80,000~80,050

C3 100,000~109,999

C4 110,000~119,999

C5 From 120,000

5-2

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Section 5 Troubleshooting

5-2.Error Indicators
(1)When trouble occurs during operation, the “ TIME" display flashes to show the error. The
error indicators are mentioned in the table below.

(2)When any of the error indicators turn on during ope ration (when the rotor is spinning),
the rotor immediately dece lerates and stops.

[Ex.]“TIME"display
Control panel
TIME
min

sec

NOTE
The equipment is inoperative while any of the error indicators is turned on.

Error Checkpoint Action taken


Indicators

① Balance the load (samples or bucket).


Imbalance error ② Remove dust and dirt from the sliding
This indicator flashes and the surface and apply grease so that the bucket
E1 rotor stops rotation when on the swing rotor swings up smoothly.
excessive vibration is Contact your local dealer for inspection when
detected. this error continues to flash even after taking
the above measures.

Lid open error Close the lid completely.


E2 This indicator lights when When the error recurs, contact your nearest
the lid is open. dealer for inspection.

After the rotor stops spinning, turn off power


Inverter Error
supply once and turn on the power after about
This indicator flashes when
E3 abnormal signal comes out
30 seconds.
When the error recurs, contact your nearest
from the inverter.
dealer for inspection.

Cont’d. on next page.


5-3

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Section 5 Troubleshooting

Error Checkpoint Action taken


Indicators

Speed sensor error After the rotor stops spinning, turn


This indicator flashes when the off power supply once and turn on
E4 self-inspection function detects the the power after about 30 seconds.
fault or abnormality in the speed When the error recurs, contact your
sensor. nearest dealer for inspection.

Over speed error


Contact your local dealer for
Flashes to stop the rotation when
E6 the maximum speed of the
inspection when this error
continues to flash.
corresponding rotor is exceeded.

Speed error
This code appears when the After the rotor stops spinning, turn
off the power supply once and turn
equipment did not start its
on the power after about 30
E7 ope ration within 5 sec onds after
seconds.
the "START" key was pressed by
If the error recurs, contact your
such causes as electric c ircuit nearest dealer for inspection.
failures.

5-4

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Section 5 Troubleshooting

5-3.Troubleshooting

  Problem    Checkpoint Action taken

Is the power plug disconnected Insert the power plug into the
1. The “STOP” from the power outlet? socket.
key lamp does
not lit even if the Is power supplied to the socket or
Inspect the power supply.
“POWER ” knife switch?
switch is turned
on. Is the power supply voltage Use a power supply in the correct
correct? (Note1) voltage range.

2. The power is
turned off imme-
diately even if Is the power supply voltage Use a power supply in the correct
the “ POWER ” correct? (Note1) voltage range.
switch is turned
on.

Pull up the “POWER”switch to


Is the “POWER”switch turn on? turn the power on.

3. The lid does not


open even if the Is the <STOP> lamp lit or Wait until the rotor is completely
“ OPEN ”button flashing? stopped.
is pushed.
Does it open when the power Check if an imperfect contact is
switch is turned off and turned not occurring with the power
on once again in 10 seconds? cable connection.

“ ”
Is the “POWER”switch turn on? Pull up the POWER switch to
turn the power on.

Go back to Problem 1. & 2. and


Is the <STOP> lamp lit?
check the power supply.
4. The motor does
not spin. Is the alarm <E1, E2, E3,
Refer to pages 5-1, 5-3 and
E4, E6, E7> flashing on the 5-4.
“ TIME”display ?

Is the lid closing? (Note 2) Close the lid firmly.

5. The speed does


not reach the set Is the power supply voltage low? Use a power supply in the correct
value even if the (Note 1) voltage range.
knob is turned.

Note 1: Read [2-4. Power Requirements] on page 2-3.


Note 2: Read [3-3. Opening and Closing the lid] on page 3-4.

5-5

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Section 5 Troubleshooting

  Problem    Checkpoint Action taken

Distribute buckets and tubes


Are buckets and tubes correctly correctly.When the error recurs,
distributed? (Note 3) contact your nearest dealer for
inspection.

Spray lubricant to buckets and


Do the buckets swing smoothly?
6. Excessive vibration. trunnion pins.
or the error <E1.> lit.
Are the rotor knob or the rotor
Fasten the rotor knob or the rotor
locking nut loose?
locking nut securely.
(Note 3)

Fasten the rotor locking bolt or


Check if the rotor is not loose.
the rotor locking nut securely.

Are buckets and tubes correctly Distribute buckets and tubes


distributed? (Note 3) correctly.
7. An abnormal noise
sounds.
Spray lubricant to buckets and
Do the buckets swing smoothly?
trunnion pins.

Note 1: Read [2-4. Power Requirements] on page 2-2.


3: Read section 6 [Rotor] on page 6-1.

  If the error still remains even after the troubleshooting is made and the action has been taken, or
in case the following phenomena are occurring, stop the operation.

● The rotor or metal tubes has been found to be damaged or corroded.


● A burnt smell comes out of the equipment.
● You receive a minor electrification when you touch the equipment body
by a naked hand.
● Some other abnormality or failure has been found occurring.

NOTE
When operation centrifuge is interrupted by some abnormality, failure or
for repairs, always turn off the “POWER”switch and attach “DO NOT
USE ” stickers to the rotor and the centrifuge before contacting your
nearest dealer.

5-6

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Rotor
Section 6
6-1.Mounting RS-240 Rotor
When you purchase the centrifuge and rotor together, the rotor will be in the
state of being mounted on the centrifuge.
When replacing the rotor or when the rotor is removed for some reason, refer
to the mounting procedure in the manual for each rotor.

  CAUTION
Make sure that the rotor yoke is firmly fastened to the drive shaft
with the rotor locking nut.
Since the rotor is not fastened onto the shaft when thes e parts are loose,excessive
vibrations will occur and damage the equipment and the rotor may fly off inflicting
physical injury.

1. Confirm that the “ Bucket intervention


prevention plate" is mounted on the drive Rotor yoke
shaft.

2. To install the rotors, match the rotor groove


with the pin of the drive shaft.
Groove
3. Fix the rotor by tightening the rotor locking Screw
nut. Rotate the nut clockwise using the Drive shaft
T-type box wrench etc. Drive pin

4. After installation of the rotor, hold the rotor Bucket interven-


yoke and attempt to move it up and down to tion prevention plate
ensure that there is no play.

Do not start the operation when Rotor loc king nut


the rotor locking nut is removed. (NOTE)

T-type box wrench

If the drive pin of the drive shaft is


not set in the groove of the rotor
bottom surface, the bucket
intervention prevention plate is
not be fixed and moves up and
down.
In such a case, re-install the
rotor. (NOTE)
T-type box wrench will be attached to the
rotor as standard accessary, when the
second rotor is purchased.
6-1

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Section 6 Rotor

6-2.Mounting RS-1004 Rotor


CAUTION
 
Make sure that the rotor yoke is firmly fastened to the drive shaft
with the rotor knob.
Since the rotor is not fastened onto the shaft when these parts are loose,excessive
vibrations will occur and damage the equipment and the rotor may fly off inflicting
physical injury.

1. Confirm that the “ Bucket


intervention prevention plate ” is Rotor knob
mounted on the drive shaft.

2.To install the rotors, match the pin


mark of the rotor with the drive Rotor yoke


pin of the drive shaft.

3. Fix the rotor yoke by rotor knob,


and then tighten by turning the mark indication to
rotor knob clockwise. align with the drive
pin at 2 places.

4. After installation of the rotor, hold Screw


the rotor yoke and attempt to move Drive shaft
it up and down to ensure that there Drive pin
is no play.
Bucket interven-
tion prevention plate

Drive pin
Center axis of rotor

Do not start it in the condition


that the rotor knob got loose. Drive shaft

Groove

If the position of the pin mark of the rotor does not align with the drive pin,

the groove at the bottom surface of the rotor and drive pin do not fit.
In such a case, reinstall the rotor.

6-2

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Section 6 Rotor

6-3.Mounting RMP-23 Rotor


CAUTION
 
Make sure that the rotor yoke is firmly fastened to the drive shaft
with the rotor locking nut.
Since the rotor is not fastened onto the shaft when these parts are loose,excessive
vibrations will occur and damage the equipment and the rotor may fly off inflicting
physical injury.

1. Confirm that the “ Bucket intervention


prevention plate ”is mounted on the drive
shaft.
T-type box wrench
(NOTE)


2.To install the rotors, match the pin mark
of the rotor with the pin of the drive shaft.
Rotor locking nut
3. Fix the rotor by tightening the rotor Rotor yoke
locking nut. Rotate the nut clockwise
using the T-type box wrench etc.


mark indication to
4. After installation of the rotor, hold the align with the drive
pin at 2 places.
rotor yoke and attempt to move it up and
down to ensure that there is no play.

Screw
Drive pin Drive shaft
Center axis of rotor Drive pin
Bucket interven-
Drive shaft tion prevention plate

Groove

(NOTE)
T-type box wrench will be attached to the
Do not start the operation when rotor as standard accessary, when the
the rotor locking nut is removed. second rotor is purchased.

If the drive pin of the drive shaft is not set in the groove of the rotor bottom
surface, the bucket intervention prevention plate is not be fixed and moves
up and down.
In such a case, re-install the rotor.

6-3

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Section 6 Rotor

6-4.RS-240 Swinging Bucket Rotor


[1]Specification
Maximum Speed (rpm) 4,
000

Maximum R C F ( × g) *1 2,
610

Maximum W eight (kg) 1.


Nomi- Maxi-
nal Num- Allow- mum Allow-
Capa- ber Tube Sizes able Tube rack / Radius able
city of Tubes Dia. × Length Tubes Speed R C F Bucket Adaptor Cushion R load
ml Tube Material mm Form Code No. rpm ×g Code No. Code No. Code No. cm gram
   *2 *3    *5

2 48 PP 9.
5~11× C 1.
5~2ml 950 053-5
1, 040 055-48
30 10.
9 50
36~60 R micro tube Carrier
024-18
80
GL 12.
5~13.
3× 053-5 030
6 40 PL 36~80

R - 1,
970 Carrier 055-48
20 11.
0 100

Becton,
5 Dickinson
GL 12.5~13.
3× Hemolyse tube
24 PL 46~105 R 430 053-58
2, 50 055-0290 024-18
90 13.
6 120
Carrier
10 052-6320

10/15 4 2,
500 053-4990 055-7400 14.
0 100
GL 15~17.
2× C 052-6360 024-1400
86~110 R 052-6330 4,
000
15 4 2,
540 053-4910 14.

30
Conical tubes 053-4910 Use bucket
15 4 PL 17×121 C 2,
580 without 14.

FALCON etc.      *4
cushion.

VENOJECT Ⅱ
15~17.
2×
etc. -
10/15 8 GL Hemolyse tube 2,
520 053-4900 024-14
00 14.

86~110 C 052-6360
R 052-6330 60

Conical tubes 053-4900 Use bucket


15 8 PL 17×121 C 2,
580     *4 without 14.

FALCON etc. cushion.

*1 The Max. RCF is the calculation value of the Max. speed for the rotation radius of 14.6cm.
The value of the RCF that is indicated to the display is showing the value that cut the lower 2 digits.
Refer to Page 3-12.
*2 PP : Polypropylene GL : Pyrex Glass PL : Plastic
*3 C : Conical R : Round type
*4 Use a bucket after removing cushion.
*5 Allowable load per bucket.
This load includes the weight of the sample, adaptor, tube rack, cushion, tube, cap, etc.
The weight of bucket is not included in the allowable load.

6-4

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Section 6 Rotor

Nomi- Maxi-
nal Num- Allow- mum Allow-
Capa- ber Tube Sizes able Tube rack / Radius able
city of Tubes Dia. × Length Tubes Speed R C F Bucket Adaptor Cushion R load
ml Tube Material mm Form Code No. rpm ×g Code No. Code No. Code No. cm gram
   *2  *3  *5

VENOJECT Ⅱ
15~17.
2× etc.
10/15 16 GL Hemolyse tube 2,
500 053-4980 024-1400 14.
0 120
86~110 C 052-6360
R 052-6330

VENOJECT Ⅱ
15~17.
2× etc.
10/15 24 GL C Hemolyse tube 2,
490 053-7340 024-2610 13.
9 500
89~105
R 052-6
     *6
330

VENOJECT Ⅱ
50 4 GL 27~37× R etc. 4,
000 2,
540 053-4990 024-3190 14.
2 100
95~110 Hemolyse tube
052-6370

024-1480
50 4 30×117 2,
560     *8 14.

Conical tubes
15 PL 17×121 C 2,
610 053-5010 14.
6 80
FALCON etc.
055-6010

     *7 -
50 30×117 2,
500 14.

*2 GL : Pyrex Glass PL : Plastic


*3 C : Conical R : Round type
*5 Allowable load per bucket.
This load includes the weight of the sample, adaptor, tube rack, cushion, tube, cap, etc.
The weight of bucket is not included in the allowable load.
*6 When use 15 ml of spitz tube (Conical) use it with 3,000 rpm or less.
*7 Be sure to remove the cushion (No. 024-1480) and then use the adaptor (Code No. 055-6010).
*8 Cushion is included with this bucket.

6-5

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Section 6 Rotor

[2]Setting Buckets

CAUTION
The same type buckets must be provided to every (4places) rotor yoke.
If not positively held in place, the rotor or centrifuge can be damaged, thereby
causing accidents.

Correct Wrong

Different types of
buckets are used.
Not all locations are provided
with a bucket.

[3]Tube distribution method

Dummy
Correct Put the dummy in to
keep the l oad
balanced.

Wrong

Only 1 tube is hangi ng

CAUTION
■ Distribute the tubes symmetrically about the center of the bucket.
・Appropriate balance is lost and violent vibrations can result if tubes are incorrectly
arranged. And, unexpected accidents can result from such violent vibrations.
・The settling chamber may be tilted since the tubes can't be maintained horizontal
during rotation.
・When you don't have enough number of tubes, dummy tubes shall be used to
maintain symmetry of the both sides.
■ Keep the load balanced symmetrically within 10 gram.

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Section 6 Rotor

[4]Cushions / Adapter
When glass tubes are used, be sure to use the cushion to avoid damage.
Insert the cushion into the tube rack so that its concave side faces
upward. Glass tube
If a glass tube is damaged, replace the cushion.
Bucket
If broken pieces of the glass tube are contained in the cushion, the
glass tube will be broken again.
Cushion

Do not insert the cushion upside down or in a


slanted position, otherwise, the glass tube may be
damaged or you may not be able to remove it.

(1)Cushion for 15ml and 50ml Glass tubes

How to place 15ml cushion

① Place a cushion in the bucket with the Cushion for 15ml Glass tube
concave surface facing upward. Code No.024-2610
(Bucket Code No.053-7340)
Code No.024-1400
② Fully insert the cushion to the bottom of the (Bucket Code No.053-4990
hole using a glass tube. 053-4910, 053-4900
(the edges of the cushion bend to prevent it 053-4980)

from coming out.) Bucket

How to place 50ml cushion


Cushion for 50ml Glass tube
  Place a cushion in the bucket with the Code No.024-3190
concave surface facing upward.

Bucket

6-7

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Section 6 Rotor

(2)Cushion for Carrier

The cushion for the carrier is shaped like a sheet.


The cushion is placed on the bottom of the carrier.

Cushion for carrier [Ex.]Carrier Code No.053-5030

Carrier Cushion
6ml Glas s tube
Code No. CodeNo.

053-5040
024-1880
053-5030

053-5850 024-1890 Cushion


Code No.024-1880

(3)Adapter for 15 ml / 50 ml conical tubes

When using the bucket (code No. 053-5010) commonly


for both a 50 ml and a 15 ml conical tube, an optional Adaptor
adapter (code No. 055-6010) must be used. Code No.055-6010

50ml conical tube 15ml conical tube

15 ml conical tube
50 ml conical tube

Bucket Bucket
Code No.053-5010 Code No.053-5010

Adaptor Adaptor
Code No.055-6010 Code No.055-6010

6-8

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Section 6 Rotor

(4)Cushion for 50 ml conical tube


The cushion (Code No.024-1480) is fitte d to
the bucke t (Code No.053-5010) and is Cushion for 50ml
exclusively used for the 50ml conical tube Code No.024-1480
(culture tube).

Bucket
Code No.053-5010

(5)How to remove cushion

① Stick the cushion with an eyeleteer or a pair


of pointed tweezers.

② Raise the cushion perpendicularly on the


bucket bottom.

③ Stick the side of the cushion to take it out.

6-9

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Section 6 Rotor

6-5.RS-1004 Swinging Bucket Rotor


[1]Specification
Maximum Speed (rpm) 4,
000
Maximum R C F ( × g) *1 2,
590
Maximum W eight (kg) 1.

Nomi- Maxi-
nal Num- Mate- Allow- mum Allow-
Capa- ber rial Tube Sizes able Tube rack Radius able
city of of Dia. × Length Tubes Speed R C F Bucket / Adaptor Cushion R load
ml Tube Tube mm Form Code No. rpm ×g Code No. Code No. Code No. cm gram
  *2  *3   *4

GL 12.5~13.
5× Becton, S13041
5 16 PL 70~110 R Dickinson 2,
500 *5 S14033 14.

Hemolyse tube
GL 11.8~12.
8×
5 20 PL 65~85 R RIA tube 2,
020 S1300
9 - 11.

GL 11.5~12.
5×
7 20 PL 95~105 VENOJECT Ⅱ 2,
400 S13004 - 13.

R etc.
7 20 GL 11.5~12.
5× Hemolyse tube 500 S1304
2, 1 S14029 14.

PL 65~105 *5
Conical tubes

15 4 PL 16.
5×120.
6 C 560 S1315
2, 0 S13060 14.

FALCON etc. *6

15 16 PL
15.2~16.
80~11
2×
0 R - 2,
500
S13041
*5 S14030 14.

Conical tubes
15 8 PL 16.
5×120.
6 C
FALCON etc.
2,
560 S1315
2 - 14.

16~17×
15 16 PL 90~105 R - 4,
000 2,
400 S1301
2 - 13.
4 350
23~24×
25 4 GL 95~100 2,
400 S1309
7 S17925 - 13.

R S15025 Cushion is
25/30 4 GL 24~25.
3× 500 S1304
2, 1 S14031 included with 14.

75~105 *5
this tube rack.
Conical tubes S13150
50 4 PL 29.
5×117 C
FALCON etc.
2,
590 *6*7 - - 14.

34~35×
50 4 GL 95~100 R 2,
400 S1309
7 S17950 - 13.

S15050
34~35× Cushion is
50 4 GL 70~105 500 S1304
2, 1
*5 S14032 included with 14.

this tube rack.
S16051
44.5~45.
5×
100 4 GL 95~105 R S1510
0 2,
400 S13097 - Cushion is 13.
included with

this bucket.

*1 The Max. RCF is the calculation value of the Max. speed for the rotation radius of 14.5cm.
The value of the RCF that is indicated to the display is showing the value that cut the lower 2 digits.
Refer to page 3-12.
*2 GL : Pyrex Glass PL : Plastic
*3 C : Conical R : Round type
*4 Allowable load per bucket.
This load includes the weight of the sample, adaptor, tube rack, cushion, tube, cap, etc.
The weight of bucket is not included in the allowable load.
*5 Sealing cap No.S17140 is included with this Bucket.
*6 Sealing cap No.S17151 is included with this Bucket.
*7 When using the adapter (S13060), 4 tubes of 15ml conical tube can be used.

6-10

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Section 6 Rotor

[2]Setting Buckets

CAUTION
The same type buckets must be provided to every (4places) rotor yoke.
If not positively held in place, the rotor or centrifuge can be damaged, thereby
causing accidents.

Correct Wrong

Different types of
buckets are used.
Not all locations are provided
with a bucket.
[3]Tube distribution method

CAUTION
■ Distribute the tubes symmetrically about the center of the rotor.
Improper distribution will cause violent vibration resulting in damage to the
centrifuge, or the rotor may come off.
■ Keep the load balanced symmetrically within 10 gram.

Correct Dummy
Put the dummy in to
keep the l oad
balanced.

Wrong

Only 1 tube is hangi ng

[4]Standard accessories
(1)Grease (SIGMA Parts No.70284) ・
・・・・・・・・・・・・・1Pc.
(2)Rotor knob・・・
・・・・ ・・ ・・・・・・・・・・・・・・・
・・・ ・
・・ ・・
・1Pc.

6-11

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Section 6 Rotor

6-6.RMP-23 Swinging Bucket Rotor


[1]Specification
Maximum Speed (rpm) 4,
000
Maximum R C F ( × g) *1 1,
790
Maximum W eight (kg) 1.

R2
RX A

R1

Number Number Allowable Radius Allowable


of of Plate Plate Sizes Speed RCF R1 load
wells Plate Material mm rpm ×g Buckets cm gram
*3 *4

2~6
96 *2 PL Bucket is
86×128×52 4,
000 1,
790 included 10 345
with this
96 2 PL rotor.

*1 The Max. RCF is the calculation value of the Max. speed for the rotation radius of 10 cm.
The value of the RCF that is indicated to the display is showing the value that cut the
lower 2 digits. Refer to Page 3-12.
*2 The maximum speed drops when the step number increases, although a micro plate is
possible to piling 3 steps. Refer to Page 6-15.
*3 PL : Plastic

*4 Allowable load per bucket. This load includes the weight of the sample, plate,
adaptor etc.
The weight of bucket is not included in the allowable load.

Calculating Centrifugal Force for RMP-23

Because of the size of the micro plate,the radius of rotation differs between the center and
the both ends with respect to the rotation axis ,
resulting in the difference in the centrifugal

force The centrifugal force of the place A (cm) Distant from the center of a bucket can be
calculated in accordance with the formula, where the rotation radius R shall be substituted
with the Rx to be obtained by the formula mentioned below. 

R(
X cm)=   R1
( cm)+A(
 2 2
cm)

where,R:1 Radius rotation Minimum(cm )

RX:Actual rotation radius in a position which is A cm away from the center of the
bucket.
6-12

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Section 6 Rotor

[2]Check the trunnion pin

CAUTION
Check the trunnion pin (4 places) is not loosened.
If the trunnion pin is loosened, the rotor or centrifuge can be damaged,
thereby causing accidents.

If the trunnion pin is loosened, contact


your local dealer for inspection.

Trunnion pin

[3]Setting Buckets

CAUTION
The same type buckets must be provided to every (2 places) rotor yoke.
If not positively held in place, the rotor or centrifuge can be damaged,
thereby causing accidents.

Correct Wrong

Load the micro plate after detaching


the bucket from the rotor.

6-13

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Section 6 Rotor

[4]Distribution of samples

CAUTION
■ Keep the load (of the sample) balanced.
If an appropriate balance is not provided, unexpected accidents can
result from a damaged rotor or centrifuge.
■ Keep the load balanced symmetrically within 5 gram.

Correct Wrong

● Sample

[5]Setting plate

CAUTION
Use the same plates.
The wrong arrangement will cause imbalance and resulting in damage
to rotor, bucket or the centrifuge.

6-14

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Section 6 Rotor

[6] Allowable weight of plate

WARNING
■ When pile up the micro plate to each bucket in 3 steps, use it after
the sufficient test for the strength of the plate is made.
The more the micro plates are piled up, the easier the plate at the
lower position becomes damaged.

■ When deep well plate is used,please ask the plate maker for the
allowable RCF of the deep well plates.

■ Allowable weight of micro plate and cell culture plate put in the
bucket is 345 gram. If it exceeds 345 gram, rectify reduced
maximum speed by the following formula.
If it is not used at the rectified speed, the rotor and the bucket will
be damaged, causing the accident.

Reduced maximum = Allowable load (gram)


speed (rpm ) Maximum speed(rpm )×
Actual load (gram)

[7]Standard accessories
Grease (SIGMA Parts No.70284) ・
・・・
・・・
・・・・
・・・・
・・・・
・・・・
・・・1Pc.

6-15

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Section 7 Specifications

7-1.Centrifuge
Max. speed 4,000 rpm
Max. RCF 2,610 × g
Max. Capacity 400ml
Invertor Microprocessor control, Speed, Centrifugal force, time,
Control system
acceleration / deceleration, 3 memory
Acceleration/Deceleration Rapid, Slow, 4 ways of switching
Lid open, Imbalance, Over speed, Function for detecting an
Alarm display occurrence of electrical abnormality in speed sensor, invertor, Lid
rock, Motor
Speed setting Digital display, 200-4,000 rpm, in 100 rpm steps
Speed indication Digital display, 0-4,300 rpm, in 100 rpm steps
Digital display, in 100 × g steps
RCF setting
10-2,600 × g(The centrifugal force differs by the rotation radius. )
Digital display, in 100 × g steps
RCF indication
0-3,000 × g(The centrifugal force differs by the rotation radius. )
From 1 second to 99 seconds (1 second step)
Timer setting range
1 minutes to 99 minutes(1 minutes step) HOLD
Setting the Sound that
informs the end of the 5 kinds + Sound none
operation
Rated voltage and Rated 100-115 V 50/60 Hz 3A
current 200-240 V 50/60 Hz 1.3 A
100-115 V 180 W 0.65 MJ/h
Total heat produced
200-240 V 170 W 0.62 MJ/h
Dimensions, Weight 35 (W) × 42(D) × 32(H) cm, 24 kg
Safety regulation IEC 61010-2-020, CLASS Ⅰ
Temperature : 10 to 40 ℃
Operation environment Atmospheric pressure : 70 to 106 kpa (700 to 1,060 mbar)
Humidity : 30 to 85%

7-2.Standard Accessories 
1. Instruction manual・
・・
・・
・・
・・
・・
・・
・・
・・1 Copy 2. Power cable・
・・
・・
・・
・・
・・
・・
・・
・・
・・
・・
・・1 Pc.

3. Rotor locking nut・


・・
・・
・・
・・
・・
・・
・・
・・1 Pc.

7-1

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Section 8. Parts List
8-1.Recommended Spare Parts
Code No. Description Use , standard Q'ty

014-0061 Micro switch VX-016-1A3 1

014-0071 Micro switch V-102-1A5 1

029-0010 Speed sensor 1

029-0009 CTL PC board Without ROM 1

066-0016 CPU ROM 1

014-0056 Invertor VFNC1S-1002P, For 100-115V 1

014-0057 Invertor VFNC1S-2002PL, For 200-240V 1

017-0009 motor KC128 0.18kW 1

014-0059 Solenoid CKD DS-08A DC5V 1

014-4470 Circuit protector W28 × Q1A-8, For 100-115V 1

014-0065 Circuit protector W28 × Q1A-5, For 200-240V 1

014-0062 Switching requireter CSJ010-05, For 100-115V 1

014-0063 Switching requireter CWA015-05, For 200-240V 1

014-0064 Power switch AJ921001WW3 1

8-1

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Index
A R
Acceleration ・ Deceleration 1-2, 3-9 Reduced maximum speed 3-18
ACCEL 1-2, 3-9 Reminder alarm 3-16
Allowable load 3-18 Reverse of setting order of
Appearance 1-1 the Speed and the Timer 3-15
Autoclaving Ⅴ Rotation Radius 3-12

C S
Calculating Centrifugal Force 3-18 sec 3-8
Centrifugal force(×g) 3-7 Sound that informs 3-14
Circuit Protector 4-6 the end of the operation
Cleaning &Sterilization 4-3, 4-4 Speed 3-6
Contaminant Elimination 4-9, 4-10 START 1-2, 3-2
Control Panel 1-1, 1-2 STOP 1-2, 3-2

D T
DECEL 1-2, 3-9, 3-13 Tachometer port 1-1, 4-7

G Timer 3-8
Greasing 4-5 U
Grounding 2-3 Using rotor Ⅳ

H Rotor
HOLD 3-8 RMP-23 6-3, 6-11, 6-12, 6-13, 6-14

I RS-1004 6-2, 6-9, 6-10


Indicators for the end RS-240
of the operation 3-17 6-1, 6-4, 6-5, 6-6, 6-7, 6-8, 6-9
Inspection 4-1, 4-2, 4-8 Troubleshooting
L 5-6, 5-7, 5-8
Life time of the rotors Ⅳ Alarm Indicators
M E0 5-1
Memory 1-2, 3-10 C1 5-2
min 3-8 C2 5-2
Movement of centrifuge 2-1 C3 5-2

O C4 5-2
Opening and Closing the lid 3-4, 3-5 C5 5-2
Operation 3-1, 3-2, 3-3 Error Indicators
P E1 5-3
Place of Installation 2-1 E2 5-3
Power Requirement 2-2 E3 5-3
E4 5-4
E6 5-4
E7 5-4

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