Oral Biofilm Activity Culture Testing and Caries Experience in School Children PDF

Oral Presentations
Oral Session O02/Cariology 2

O02–9 Regional Committee for Medical Research Ethics and The
Investigation of dental health indices and caries Norwegian Social Science Data Services.
Results: Having missed dental appointments before the age of five
associated microflora in children with cleft lip and (OR = 4.7), behaviour management problems (OR = 3.3), child
palate dental anxiety (OR = 3.1) and parents reporting no access to
N. HUROGLU & I. TANBOGA dental care (OR = 2.1) were bivariately associated with caries
Pediatric Dentistry Department Dentistry Faculty of Marmara experience. In multivariate logistic regression, having missed dental
University, Istanbul, Turkey appointments (OR = 4.0) and parents reporting behaviour man-
Introduction: In children with oral cleft, a healthy primary agement problems before age of five (OR = 2.4) were statistically
dentition is important because of the increased need of ortho- significant risk indicators for having caries experience at the age of
dontic treatment during the primary dentition and transitional five, when controlling for parents education level and national
period. Early extractions or malformations of teeth will result in origin.
loss of bone preserved by primary teeth bordering the alveolar Conclusion: Behaviour management problems during previous
cleft. The purpose of the present investigation was to assess the dental visits and parents that avoid bringing their child to
prevalence of Streptococcus mutans and lactobacilli in 3–5 year- scheduled dental appointments represent risk indicators for
old children with cleft lip and palate attending Pediatric dental caries in 5-year-old children.
Dentistry department of Marmara University and to determine
the possible factors associated with the colonization of these O02–11
microorganisms.
Patients and methods: 45 children with cleft lip and palate and 34 Oral biofilm activity, culture testing and caries
healthy controls (21 decayed, 13 caries free) matched for sex and experience in school children
age attending the same department participated in the study. All K. B. HALLETT1 & P. K. OÕROURKE2
1
subjects accompanied by their parents were called for a clinical and Royal Children’s Hospital; 2QLD Institute of Medical Research,
radiological examination and a saliva sample collection. The Brisbane, Australia
parents were interviewed with a structured questionnaire.
Introduction: To evaluate a chair side caries assessment protocol
Results: The mean caries experience (dmft) was 6.42 ± 5.48. The
utilising an oral health questionnaire, oral biofilm activity, culture
dental anomalies most seen in children with cleft were: rotation
testing and routine dental examination in 5–12 year-old children at
(60%), hypodontia (37.8%) and enamel alterations (33.3%). In
two regional schools.
children with cleft lip palate the values of MS, LB and Candida
Patients and methods: Permission was obtained from regional
were high. Salivary secretion rate was significantly low especially in
hospital and school ethics committees and informed consent was
children who used dental appliance.
given by a parent or guardian for each volunteer child participant.
Conclusion: These results underline the need for a more intensive
Parents were interviewed by telephone and completed an inter-
approach to prevention of oral disease in children with cleft lip and
viewer assisted oral health questionnaire regarding current and
palate to optimize clinical outcome. Therefore a pediatric dentist
previous child oral health behaviours. Oral biofilm was sampled
responsible for initiating the preventive program should be
from the labial and buccal surface of a maxillary incisor and the
included in the cleft team.
lingual surface of the mandibular incisor teeth using a sterile cotton
swab. Biofilm activity was measured directly in relative light units
O02–10 (0–9999) using an adenosine triphosphate (ATP) bioluminescence
meter. Bacterial counts were recorded after 48 h incubation by
Avoidance behaviours as risk indicators for dental counting the highest density of colony forming units (Low,
caries in 5-year-old children Medium and High). Each child’s dentition was examined clinically
T. I. WIGEN, E. SKARET & N. J. WANG and radiographically and their current caries experience recorded
Institute of Clinical Dentistry, Department of Paedodontics, using dmfs and DMFS indices. Cross tabulations of selected
University of Oslo, Oslo, Norway categorical variables and grouped caries indices were performed
Introduction: The objective of the study was to explore the using a Pearson Chi Square analysis.
associations between dental anxiety and dental avoidance behav- Results: Caries experience was significantly associated with
iour in parents and children and caries experience in 5-year-old oral biofilm activity (RLU < 9000, 9000–9499, > 9500) in 292
children. It was hypothesized that parentsÕ self-reports of dental children examined to date (P = 0.3). Bacterial counts and oral
anxiety and lack of access to dental care were related to child health behaviours were not significantly associated with caries
dental health and thereby could be assessed as risk indicators for indices.
dental caries in 5-year-old children. Conclusions: Oral biofilm activity rather than bacterial counts is
Materials and methods: Data were collected from the dental significantly prognostic of baseline caries indices in this school
records and by clinical and radiographic examination of 523 child population. This study was supported by a Queensland
children. The parents completed a questionnaire regarding government clinical research grant.
socioeconomics, dental anxiety, dental attendance and behaviour
management problems. Bivariate and multivariate logistic
regression was conducted. A written, informed consent was
obtained from all parents. The investigation was approved by the
ª 2009 The Authors

4 Journal compilation ª 2009 BSPD, IAPD and Blackwell Publishing Ltd, International Journal of Paediatric Dentistry 19 (Suppl. 1): 1–65

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Oral Session O02/Cariology 2

ª 2009 The Authors

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