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    Assignment 1 HPLC

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    Muhammad Bin Ismail
    This document describes using HPLC to determine the initial concentration of caffeine in an energy drink using two methods: peak height and peak area. For peak height, the initial concentration was calculated to be 9.798 mg/ml. For peak area, the initial concentration was calculated to be 0.0214 mg/ml. Peak area is considered a more reliable method for HPLC because it is not affected by an unsymmetrical peak shape or baseline noise, which tend to be higher for HPLC compared to GC.

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    Assignment 1 HPLC

    Uploaded by

    Muhammad Bin Ismail
    22 views3 pages
    This document describes using HPLC to determine the initial concentration of caffeine in an energy drink using two methods: peak height and peak area. For peak height, the initial concentration was calculated to be 9.798 mg/ml. For peak area, the initial concentration was calculated to be 0.0214 mg/ml. Peak area is considered a more reliable method for HPLC because it is not affected by an unsymmetrical peak shape or baseline noise, which tend to be higher for HPLC compared to GC.

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    This document describes using HPLC to determine the initial concentration of caffeine in an energy drink using two methods: peak height and peak area. For peak height, the initial concentration was calculated to be 9.798 mg/ml. For peak area, the initial concentration was calculated to be 0.0214 mg/ml. Peak area is considered a more reliable method for HPLC because it is not affected by an unsymmetrical peak shape or baseline noise, which tend to be higher for HPLC compared to GC.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    22 views3 pages

    Assignment 1 HPLC

    Uploaded by

    Muhammad Bin Ismail
    This document describes using HPLC to determine the initial concentration of caffeine in an energy drink using two methods: peak height and peak area. For peak height, the initial concentration was calculated to be 9.798 mg/ml. For peak area, the initial concentration was calculated to be 0.0214 mg/ml. Peak area is considered a more reliable method for HPLC because it is not affected by an unsymmetrical peak shape or baseline noise, which tend to be higher for HPLC compared to GC.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 3

    Initial concentration of diluted caffeine at standard solution

    (0.50 ml)(1.205 mg/ml)


    at addition of 0.50 ml=
    50 ml
    at addition of 0.50 ml=0.01205 mg/ml

    (2.00 ml)(1.205 mg/ml)


    at addition of 2.00 ml=
    50 ml
    at addition of 2.00 ml=0.0482mg/ml

    a. Original concentration of caffeine in energy drink using peak height

    peak height of caffeine at 0.50 ml addition=46.77−24.7 9


    peak height of caffeineat 0.50 ml addition=21.9 8

    peak height of caffeineat 2.00 ml addition=80.40−24.79


    peak height of caffeine at 2.00 ml addition=55.6 1

    X (mg/ml) 0.01205 0.0482


    Y 21.98 55.61

    Peak height of caffeine


    60

    f(x) = 2.53 x
    50

    40
    Peak height

    30

    20

    10

    0
    0 5 10 15 20 25
    Concentration of caffeine
    initial concentration of caffeine in energy drink @ 24.79 peak height,

    y=2.53 x

    24.79=2.53 x

    x=9.798 mg/ml

    b. Original concentration of caffeine in energy drink using peak height

    peak area of caffeineat 0.50 ml addition=831.7−408.3


    peak area of caffeineat 0.50 ml addition=423.4

    peak area of caffeineat 2.00 ml addition=1278.6−408.3


    peak area of caffeineat 2.00 ml addition=870.3

    X (mg/ml) 0.01205 0.0482


    Y 423.4 870.3

    Peak area of caffeine at different concentration


    1000
    900
    f(x) = 19060.78 x
    800
    700
    600
    Peak area

    500
    400
    300
    200
    100
    0
    0.01 0.02 0.02 0.03 0.03 0.04 0.04 0.05 0.05 0.06
    Concentration

    initial concentration of caffeine in energy drink @ 408.3 peak area,

    y=19061 x

    408.3=19061 x

    x=0.0214 mg/ ml
    c. Two values calculated in a and b, which is more reliable.

    Both methods can be used to measure concentration of caffeine in energy


    drink. However, peak height and peak area differs in terms of representation of the
    data. Peak height usually needs to have a perfectly symmetrical shape (Figure 1) so
    that it can be more accurate. Also, the baseline noise also should be low. In HPLC,
    baseline noise usually higher than GC. Meanwhile, for peak area in HPLC, it can
    represent an accurate reading as it does not affect much by the unsymmetrical shape
    of the peaks.

    Figure 1

    Hence, it can be concluded that peak area is more reliable as in HPLC because of its
    ability to ignore baseline noise that usually more higher than GC.

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