Certified Reference

Materials
for
UV, Visible, NIR and IR
Molecular Spectroscopy
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RM-0204060810
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Set Serial No:

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Customer Details:
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The customer information stated on this page,

number 1 of 3 applies to all certificates.

UKAS accreditation applies to all

Wavelength,
Transmission/Absorbance, Stray
Light references, and those used for
Resolution measurements.
 Certificate of Calibration and Traceability
Calibration Lab. Potassium Dichromate in Perchloric acid sealed in
Starna Scientific Ltd Far UV quartz cells for use as a linearity and
52/54 Fowler Rd photometric accuracy reference in the UV.
HAINAULT
Essex IG6 3UT Certificate Number: 58496
Certificate Date: 27 July 2016
England Expiration Date: 27 July 2018
Tel. +44 (0) 20 8501 5550 Analysis Number: 20512 0659
Set Serial Number: 23088
Email: [email protected] Blank Serial Number: 53553 Page Number 2 of 3

Description of Reference Material:

NIST SRM 935a Potassium Dichromate is used to prepare the reference solutions.These are sealed by heat fusion in
high quality Far UV quartz cells. Certification is performed in accordance with the instructions that are issued with
NIST SRM 935a.All procedures are implemented in accordance with ISO/IEC 17025 and ISO 17034. Additional
information can be found on the Starna web site at www.starna.com

Certified Values of Reference Material:

The Potassium Dichromate filled cells are measured against a Perchloric acid blank. The net absorbance values are
listed in the table below. Under the analytical procedures used, as outlined by NIST in the Appendix NIST Special
Publication 260-54.
The combined analytical and instrument uncertainties at a coverage probability of 95 % is 0.0037 A at 20 mg/l, 0.0045 A
at 40 mg/l, 0.0049 A at 60 mg/l, 0.0058 A at 80 mg/l, 0.0068 A at 100 mg/l, 0.0084 A at 120 mg/l, 0.0091 A at 140 mg/l,
0.0098 A at 160 mg/l, 0.011 A at 180 mg/l, 0.012 A at 200 mg/l, 0.013 A at 220 mg/l, 0.013 A at 240 mg/l and 0.0043 at 600
mg/l.
The reported expanded uncertainty is based on a standard uncertainty multiplied by a coverage factor k=2.
providing a coverage probability of approximately 95%. The uncertainty evaluation has been carried out in
accordance with UKAS requirements.
The weight shown below is the mean calculated weight of potassium dichromate in this solution using the specific
absorbance values quoted on the NIST SRM 935a certificate, together with the certified absorbance values.
Nominal Concentration: Wavelength: Absorbance: Calculated Weight:
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Potassium Dichromate 20 mg/l 350 nm 0.2097

313 nm 0.0945 mg/l ± 0.5 mg/l (k=2)
Cell Serial No: 57461 257 nm 0.2818
235 nm 0.2433 19.78

Potassium Dichromate 40 mg/l 350 nm 0.4273

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313 nm 0.1946 mg/l ± 0.5 mg/l (k=2)

Cell Serial No: 55831 257 nm 0.5763
235 nm 0.4993 40.42
Potassium Dichromate 60 mg/l 350 nm 0.6424
313 nm 0.2924 mg/l ± 0.5 mg/l (k=2)
Cell Serial No: 59045 257 nm 0.8681
235 nm 0.7529 60.70
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Potassium Dichromate 80 mg/l 350 nm 0.8571

313 nm 0.3889 mg/l ± 0.5 mg/l (k=2)
Cell Serial No: 57509 257 nm 1.1589
235 nm 1.0014 80.68

Potassium Dichromate 100 mg/l 350 nm 1.0759

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313 nm 0.4851 mg/l ± 0.5 mg/l (k=2)

Cell Serial No: 47162 257 nm 1.4582
235 nm 1.2546 100.88

This certification is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to recognised national standards, and to units of measurement realised at the National Physical Laboratory or other recognised standards laboratories.
Set Serial Number: 23088 Page Number 3 of 3
Starna Certificate No: 58496
Certificate Date: 27 July 2016 UKAS Accredited Calibration Laboratory No. 0659
Analysis Date: 27 July 2016

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of high performance This certificate is valid for a maximum period of two years from
reference spectrophotometers. The instruments are tested and the date of issue or sooner if specified by the user's own protocols.
qualified to the manufacturer's published specification over the Although the references are covered by a lifetime guarantee this is
analytical range used for the reference material certification. subject to certain conditions, see guidance notes.
The following primary references and fundamental procedures are
used in the qualification of the reference spectrophotometers:
Re-certification Procedure:
Absorbance: NIST SRM 2031, 1930 & 930e, Double aperture method
All reference materials are certified and supplied in a useable
Wavelength: NIST SRM 2034, Emission lines of Hg & deuterium
condition. There is no warranty for fitness beyond receipt by the
Stray Light: NIST SRM 2032, KCl, KI & lithium carbonate
customer. When references need to be re-certified or inspected for
Resolution: Benzene vapor, half width of D2 656.1 nm line any reason, customers should return them to the Starna ISO/IEC
17025 & ISO 17034 accredited calibration laboratory, where all
Calibration Method: original data is collated.
The conditions of analysis used to generate the certified values on
this certificate are as listed in the chart below: On receipt by Starna Scientific the references are measured “As
received”, before cleaning under the re-certification procedure.
Cell Pathlength: 10 mm +/- 0.01mm “As received” data is available on request.
Cell Material: Spectrosil Quartz
Blank Solution: 0.001M Perchloric acid
Scale: Absorbance
Storage and Care:
Range: 350 to 235 nm References should always be stored in the box provided and handled
Band width: 1.0 nm +/- 0.2nm with extreme care. Quartz cells are fragile and should be inserted
Temperature: 23.5 +/- 1.0 °C and removed from the instrument by holding the cell cap, taking
care not to twist or apply leverage against the cell holder, as this
Instructions for Use: may crack the cells. Damage in the form of scratches may alter
Determine the absorbance of each cell against the supplied blank at the certified values significantly such that they need re-certifying
each of the four listed wavelengths. Repeat several times.To test and may, as with cracks, require complete replacement. For
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instrument linearity, plot the results on a graph of absorbance vs cleaning see guidance notes.
concentration. The graph should produce straight lines if your
instrument is linear in the region. To assess photometric accuracy,
compare the net absorbance reading at each concentration and Calibration performed by:
wavelength to the published values on this certificate.
The absolute difference between the mean measured value and the
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certified value will not exceed the sum of the certified uncertainty
and the specified accuracy of the instrument, if the instrument is A. Wakelin CSci CChem MRSC
performing correctly

Instrument Dependencies: Approved Signatory:

The instrument must be designed to be used in the ultraviolet

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region down to 230nm and have a spectral bandpass of 1.6nm or

less. Consult your instrument owners manual for this information. J. P. Hammond CSci CChem FRSC
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All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing
laboratory This certification must not be used by a third party to claim product endorsement by the accreditation body or any agency of
the U.S. or U.K. governments.

This certification is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to recognised national standards, and to units of measurement realised at the National Physical Laboratory or other recognised standards laboratories.