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Biodegradability Test

This document summarizes standards and guidelines for testing the biodegradability of substances. It describes applying tests to determine if substances can be broken down by microorganisms in sewage sludge or other media. Tests involve adding the test substance to inoculated mineral salts solutions and measuring biodegradation over time, following standards like ISO 11734, ISO 14593, and OECD 301. The tests are restricted for volatile, inhibitory, or poorly soluble substances and reliable analytical methods are required to measure biodegradation.

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giuseppe tropiano
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115 views1 page

Biodegradability Test

This document summarizes standards and guidelines for testing the biodegradability of substances. It describes applying tests to determine if substances can be broken down by microorganisms in sewage sludge or other media. Tests involve adding the test substance to inoculated mineral salts solutions and measuring biodegradation over time, following standards like ISO 11734, ISO 14593, and OECD 301. The tests are restricted for volatile, inhibitory, or poorly soluble substances and reliable analytical methods are required to measure biodegradation.

Uploaded by

giuseppe tropiano
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BIODEGRABILITY
ISO 11734, ISO 14593, OECD 301 - GLP
Application field

This test is applicable to readily and poorly soluble and volatile substance.

Interests

The purpose of this test is to determine biodegradability (aerobic or anaerobic) of the


test substance in sewage sludge (aerobic or anaerobic) solution.

Principle of the test

The test substance is added to mineral salts medium inoculated with mixed
population of micro-organism and incubated for a time required in each guideline.

Normative references

ISO 11734 – Water Quality – Evaluation of the “ultimate” anaerobic biodegradability


of organic compounds in digested sludge – Method by measurement of the biogas
production.
ISO 14593 – Water Quality – Evaluation of the “ultimate” aerobic biodegradability of
organic compounds in aqueous medium – Method by analysis of inorganic carbon in
sealed vessels (CO2 headspace test).
OECD Guidelines for testing of Chemicals – Ready Biodegradability. N° 301 –
17/07/1992.

Restrictions
 For substances that inhibit micro-organisms growth
 For volatile substance a case by case decision is necessary
 For chemicals of low solubility in water, the maximum concentration should
not exceed the limit of solubility in the dilution water
 A reliable analytical method for the quantification of organic carbon in the test
substance in the test solution must be available

Expression of results

Percentage of biodegradation of the test substance respect to a reference substance.

Number of products/Quantity necessary to the analysis

Quantity on request with the following information:


 name of the product;
 batch number and expiry data;
 storage and stability conditions;
[email protected]  qualitative/quantitative composition;
www.eurofins.it  certificate of analysis and water solubility;
Tel. + 39 02 2507151  pKa value and n-Octanol/water partition coefficient
Fax + 39 02 25071599 EurofinsFarma_mktg_50021
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