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    Blood Typing (Major Performance Task)

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    Harmanjot Kaur
    This document describes a laboratory activity to determine blood type. Students will perform an ABO blood typing test using slide and reagent methods to identify their own blood type based on the presence or absence of A and B antigens. The objectives are to identify one's own blood type and learn how to perform the ABO blood typing test. Materials needed include laboratory coats, slides, blood samples, alcohol, applicators, and anti-A and anti-B reagents. Students will place blood and reagents on slides, check for agglutination, and interpret results to determine their blood type.

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    Blood Typing (Major Performance Task)

    Uploaded by

    Harmanjot Kaur
    103 views2 pages
    This document describes a laboratory activity to determine blood type. Students will perform an ABO blood typing test using slide and reagent methods to identify their own blood type based on the presence or absence of A and B antigens. The objectives are to identify one's own blood type and learn how to perform the ABO blood typing test. Materials needed include laboratory coats, slides, blood samples, alcohol, applicators, and anti-A and anti-B reagents. Students will place blood and reagents on slides, check for agglutination, and interpret results to determine their blood type.

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    This document describes a laboratory activity to determine blood type. Students will perform an ABO blood typing test using slide and reagent methods to identify their own blood type based on the presence or absence of A and B antigens. The objectives are to identify one's own blood type and learn how to perform the ABO blood typing test. Materials needed include laboratory coats, slides, blood samples, alcohol, applicators, and anti-A and anti-B reagents. Students will place blood and reagents on slides, check for agglutination, and interpret results to determine their blood type.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    103 views2 pages

    Blood Typing (Major Performance Task)

    Uploaded by

    Harmanjot Kaur
    This document describes a laboratory activity to determine blood type. Students will perform an ABO blood typing test using slide and reagent methods to identify their own blood type based on the presence or absence of A and B antigens. The objectives are to identify one's own blood type and learn how to perform the ABO blood typing test. Materials needed include laboratory coats, slides, blood samples, alcohol, applicators, and anti-A and anti-B reagents. Students will place blood and reagents on slides, check for agglutination, and interpret results to determine their blood type.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    ANGELES UNIVERSITY FOUNDATION

    INTEGRATED SCHOOL
    Angeles City

    Name: Score:
    Grade level and Section: Date:

    Laboratory Activity: Blood Typing

    How do we determine different blood types? There are special traits on the red blood cells of humans
    that determine the blood type. These traits are called antigens. There are two types of antigens that blood can
    have: A antigens and B antigens. These antigens organize blood into four groups: A, B, O, or AB. The blood is
    identified as Type A if it only has A antigens. Type B blood if it only has B antigens. Type AB blood has both A
    and B antigens, and Type O blood has neither A nor B antigens. Besides the A and B antigens, there is another
    trait that blood has, to identify its type. It is called the Rh factor. The Rh factor determines whether the person's
    blood type is positive or negative. If blood has the Rh factor, it is positive. If it does not, it is negative.

    I. Objectives
    At the end of the laboratory activity, the learner shall be able to:
     identify one’s/own blood type; and
     perform ABO Red Blood Cell Testing using Slide Method.

    II. Materials
    Laboratory Gown or Coat Pricker and Lancets Blood Grouping Reagents
    Glass Slides 70% Ethyl Alcohol (Anti-sera A and B)
    Permanent Marker Gauze Pad or Cotton Ball Wooden Applicator Sticks

    III. Procedures
    1. Divide the reaction surface of a clean glass side into two sections by placing a mark or line on the
    back side using a permanent marker. Label the first and second sections with A and B
    respectively.
    2. Obtain capillary blood. Use appropriate finger, rub the puncture site vigorously with a gauze pad
    or cotton ball moistened with 70% Ethyl Alcohol.
    3. Wipe the area using gauze pad or cotton ball and let it dry.
    4. Using the pricker with sterile lancet, make a deep puncture alongside the fingertip, midway
    between the edge and midpoint of the fingertip.
    5. Using a dry gauze or cotton ball, wipe away the first drop of blood to avoid tissue contamination.
    6. Apply moderate pressure, approximately 1 cm behind the site of puncture to allow the oozing out
    of blood.
    7. Release the pressure immediately to allow recirculation of the blood, then repeat the application
    of pressure until ample amount of blood is collected.
    8. Place one drop on each section on the glass slide.
    9. Place anti-sera A beside the drop of blood in section A.
    10. Place anti-sera B beside the drop of blood in section B.
    11. Mix using applicator stick, let it stand for approximately two minutes then examine for agglutination
    macroscopically. Read and interpret the results.

    IV. Illustration
    After performing all of the procedures, take a picture of your overall output (blood samples with
    Anti-Sera A, B and D on a glass slide).

    V. Guide Questions

    1. Based on the activity, what is your blood type?


    ____________________________________________________________________________
    ____________________________________________________________________________

    2. You can donate your blood to person/s with what blood type/s? Elaborate your answer.
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________

    3. You can receive blood from person/s with what blood type/s? Elaborate your answer.
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________

    4. Why does categorizing blood according to types crucial?


    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________

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