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com

AIM
fteasuring the Amount of Acetic Acid
In Vinegar
by Titration with an
Indicator Solution
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This is to certify that ftohit K.Das of class XII has

completed the chemistry project entitled
‘DETERftINATION OF AftOUNT OF ACETIC
ACID IN VINEGAR’ himself and under my guidance.
The progress of the project has been continuously
reported and has been in my knowledge consistently.

ftrs. Aditi Kapoor

(P.G.T CHEftISTRY)
ftOTHER DIVINE PUBLIC SCHOOL
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It gives me great pleasure to express my gratitude

towards our chemistry teacher ftrs. ADITI KAPOOR
for her guidance, support and encouragement
throughout the duration of the project. Without
her motivation and help the successful completion of
this project would not have been possible.

Navi Arora

XII
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Index
1 Certificate
2 Acknowledgement
3 Aim
4 Objective
5 Introduction
6 ftaterials and Equipment
7 Theory
8 Experimental Procedure
 Experiment 1
 Experiment 2
 Experiment 3
9 Result
10 Precautions
11 Bibliography
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Objective
The goal of this project
is to determine the amount of Acetic Acid in
different types of vinegar
using titration with a coloured
pH indicator to determine
the endpoint.
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Introduction
Vinegar is a solution made from the fermentation of ethanol
(CH3CH2OH), which in turn was previously fermented from sugar. The
fermentation of ethanol results in the production of acetic acid
(CH3COOH). There are many different types of vinegar, each starting
from a different original sugar source (e.g., rice, wine, malt, etc.). The
amount of acetic acid in vinegar can vary, typically between 4 to 6% for
table vinegar, but up to three times higher (18%) for pickling vinegar.

In this project, we will determine the amount of acid in different

vinegars using titration, a common technique in chemistry. Titration is a
way to measure the unknown amount of a chemical in a solution (the
titrant) by adding a measured amount of a chemical with a known
concentration (the titrating solution). The titrating solution reacts with
the titrant, and the endpoint of the reaction is monitored in some way.
The concentration of the titrant can now be calculated from the
amount of titrating solution added, and the ratio of the two chemicals
in the chemical equation for the reaction.

To measure the acidity of a vinegar solution, we can add enough

hydroxyl ions to balance out the added hydrogen ions from the acid.
The hydroxyl ions will react with the hydrogen ions to produce water. In
order for a titration to work, we need three things:

1. a titration solution (contains hydroxyl ions with a precisely known

concentration),
2. a method for delivering a precisely measured volume of the
titrating solution, and
3. a means of indicating when the endpoint has been reached.

For the titrating solution, we'll use a dilute solution of sodium

hydroxide (NaOH). Sodium hydroxide is a strong base, which means
that it dissociates almost completely in water. So for every NaOH
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molecule that we add to the solution,we can expect to produce a

hydroxyl ion.

To dispense an accurately measured volume of the titrating solution,

we will use a burette. A burette is a long tube with a valve at the
bottom and graduated markings on the outside to measure the volume
contained in the burette. The burette is mounted on a ring stand,
directly above the titrant solution (as shown in the picture).

Solutions in the burette tend to creep up the sides of the glass at the
surface of the liquid. This is due to the surface tension of water. The
surface of the liquid thus forms a curve, called a meniscus. To measure
the volume of the liquid in the burette, always read from the bottom of
the meniscus.

In this experiment, we will use an indicator solution called

phenolphthalein. Phenolphthalein is colourless when the solution is
acidic or neutral. When the solution becomes slightly basic,
phenolphthalein turns pinkish, and then light purple as the solution
becomes more basic. So when the vinegar solution starts to turn pink,
we know that the titration is complete.
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Materials and
Equipment
To do this experiment we will need the following materials and
equipment:

 Vinegar, three different types.

 Distilled water

 Small funnel

 0.5% Phenolphthalein solution in alcohol (pH indicator solution)

 0.1 M sodium hydroxide solution

 125 mL Conical flask

 25 or 50 mL burette

 10 mL graduated cylinder

 Ring stand

 Burette clamp
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Theory
 Required amount of sodium hydroxide (NaOH) can be calculated
using the following formula:
Molarity  Molarmass Volume(cm3 )
W 
1000
Molar mass of NaOH = 40 g/mol
0.5  40  500
=
1000
= 10 g

 The acetic acid content of a vinegar may be determined by

titrating a vinegar sample with a solution of sodium hydroxide
of known molar concentration (molarity).

CH3COOH(aq) + NaOH(aq) --> CH3COONa(aq) + H2O(l)

(acid) + (base) --> (salt) + (water)

At the end point in the titration stoichiometry between the both

solution lies in a 1:1 ratio.
MCH COOH VCH COOH 1
3 3

MNaOHVNaOH 1

 Strength of acid in vinegar can be determined by the following

formula:
Strength of acetic acid = MCH COOH  60
3

Indicator:- Phenolphthalein
End Point:- Colourless to pink
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Experimental
Procedure
Performing the Titration

1. Pour 1.5 ml of vinegar in an Conical flask.

2. Add distilled water to dissolve the vinegar so that the volume of

the solution becomes 20 mL.

3. Add 3 drops of 0.5% phenolphthalein solution.

4. Use the burette clamp to attach the burette to the ring stand. The
opening at the bottom of the burette should be just above the
height of the Conical flask we use for the vinegar and
phenolphthalein solution.

5. Use a funnel to fill the burette with a 0.1 M solution of sodium

hydroxide.

6. Note the starting level of the sodium hydroxide solution in the

burette. Put the vinegar solution to be titrated under the burette.

7. Slowly drip the solution of sodium hydroxide into the vinegar

solution. Swirl the flask gently to mix the solution, while keeping
the opening underneath the burette.

8. At some point we will see a pink colour in the vinegar solution

when the sodium hydroxide is added, but the colour will quickly
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disappear as the solution is mixed. When this happens, slow the

burette to drop-by-drop addition.

9. When the vinegar solution turns pink and remains that colour
even with mixing, the titration is complete. Close the tap (or pinch
valve) of the burette.

10. Note the remaining level of the sodium hydroxide solution in the
burette. Remember to read from the bottom of the meniscus.

11. Subtract the initial level from the remaining level to figure out
how much titrating solution we have used.

12. For each vinegar that we test, repeat the titration at least three
times.
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EXPERIMENT – 1
I. Take the household vinegar in the conical flask and do the
titration with sodium hydroxide (NaOH) as mentioned.

OBSERVATIONS

S.no Volume of Burette Reading Volume of

vinegar NaOH
Initial Final
solution solution
(in mL) (in mL)
used

1. 20 0 27 27

2. 20 0 27 27

3. 20 0 27 27

Concordant volume = 27 mL

CALCULATIONS

We know that,

MCH COOH VCH COOH  MNaOHVNaOH

3 3

MNaOHVNaOH
M 
CH 3 COOH
VCH COOH
3

0.5 27
 MCH COOH  3
20
= 0.675 mol/L

Strength of acetic acid= 0.675 60

=40.5 g/L
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EXPERIMENT – 2
I. Take the wine vinegar in the conical flask and do the titration with
sodium hydroxide (NaOH) as mentioned.

OBSERVATIONS

S.no Volume of Burette Reading Volume of

vinegar NaOH
Initial Final
solution solution
(in mL) (in mL)
used

1. 20 0 48 48

2. 20 0 48 48

3. 20 0 48 48

Concordant volume = 48mL

CALCULATIONS

We know that,

MCH COOH VCH COOH  MNaOHVNaOH

3 3

MNaOHVNaOH
M 
CH 3 COOH
VCH COOH
3

0.5 48
 M CH COOH 3
20
= 1.2 mol/L

Strength of acetic acid=1.2  60

=72 g/L
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EXPERIMENT – 3
I. Take the fruit(Persimmon) vinegar in the conical flask and do the
titration with sodium hydroxide (NaOH) as mentioned.

OBSERVATIONS

S.no Volume of Burette Reading Volume of

vinegar NaOH
Initial Final
solution solution
(in mL) (in mL)
used

1. 20 0 32 32

2. 20 0 32 32

3. 20 0 32 32

Concordant volume = 32 mL

CALCULATIONS

We know that,

MCH COOH VCH COOH  MNaOHVNaOH

3 3

MNaOHVNaOH
M 
CH 3 COOH
VCH COOH
3

0.5 32
 M CH COOH 3
20
= 0.8 mol/L

Strength of acetic acid= 0.8  60

=48 g/L
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Result
 Strength of acetic acid in household vinegar = 40.5 g/L.

 Strength of acetic acid in wine vinegar = 72 g/L.

 Strength of acetic acid in fruit vinegar = 48 g/L.

Graphically plotting various vinegar samples in

accordance with the amount of acetic acid present in
them we present a stunning find:
Strenth of Different Vinegars

80

70

60

50

40

30

20

10

0
Household Vinegar Wine Fruit Vinegar

Order of amount of acetic acid in different samples

of vinegar is:
Wine > Fruit vinegAr > Household vinegAr
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Precautions
 Transference of measured vinegar into a measuring
flask should be done very carefully.
 Measuring must be performed carefully.
 Look at the meniscus of solution at eye level to avoid
parallax.
 Look at the lower meniscus in the light coloured
solution and upper meniscus in the dark coloured
solution because of visibility.
 Do not forget to add distilled water to the vinegar.
Bibliography
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