UV-VIS ANALYST

SOFTWARE MANUAL

Drawell Scientific
 TABLE OF CONTENTS
1. Functions…………………………………………………………………………………………………… 1
1.1 Main Functions……………………………………………………………………………………………. 1
1.2 Spectrum Processing Function………………………………………………………………………….. 1
1.3 System Check and Calibration Function……………………………………………………………….. 2

2. Setup…………………………………………………………………………………………………………. 3
2.1 System Requirements for the UV-Vis Analyst………………………………………………………….. 3
2.2 Setup the UV-Vis Analyst to PC…………………………………………………………………………. 3
2.3 Remove the UV-Vis Analyst from PC…………………………………………………………………… 4
2.4 Install the dongle………………………………………………………………………………………….. 4
2.5 Run the UV-Vis Analyst…………………………………………………………………………………... 4
2.6 Set Comm. Port…………………………………………………………………………………………… 5

3. Introduction………………………………………………………………………………………………... 6
3.1 Main Interface……………………………………………………………………………………………... 6
3.2 Menu bar and Toolbar…………………………………………………………………………………….. 6

4. Operation…………………………………………………………………………………………………… 11
4.1 Single Wavelength Photometric Measurement………………………………………………………... 11
4.2 Fixed Point Measurement………………………………………………………………………………... 11
4.2.1 Multi-wavelength Photometric Measurement……………………………………………………... 11
4.2.2 Concentration Measurement……………………………………………………………………….. 13
4.2.2.1 Set Up Linear Regression Curve…………………………………………………………….. 13
4.2.2.2 Measure Concentration by using the linear regression curve…………………………….. 15
4.2.3 Assistant functions…………………………………………………………………………………… 16
4.2.3.1 Delete a Result…………………………………………………………………………………. 16
4.2.3.2 Modify a Result…………………………………………………………………………………. 16
4.2.3.3 Recalculate Concentration……………………………………………………………………. 16
4.2.3.4 Set Data Font…………………………………………………………………………………… 16
4.2.3.5 Edit Measurement Information……………………………………………………………….. 16
4.3 Wavelength Scanning……………………………………………………………………………………. 16
4.3.1 Scan Sample…………………………………………………………………………………………. 16
4.3.2 Spectrum Processing………………………………………………………………………………... 18
4.3.2.1 Auto List Peaks and Valleys…………………………………………………………………... 18
4.3.2.2 Rescale…………………………………………………………………………………………. 18
4.3.2.3 Original Scales…………………………………………………………………………………. 18
4.3.2.4 Zoom Selected Area…………………………………………………………………………… 18
4.3.2.5 Trace a Spectrum………………………………………………………………………………. 19
4.3.2.6 Select a Spectrum as Current………………………………………………………………… 20
4.3.2.7 Derivative……………………………………………………………………………………….. 20
4.3.2.8 Moving Window Averaging……………………………………………………………………. 21
4.3.2.9 Savitzky-Golay Smoothing Filter……………………………………………………………... 22
4.3.2.10 Resample……………………………………………………………………………………….. 22
4.3.2.11 Spectrum Addition……………………………………………………………………………… 23
4.3.2.12 Spectrum Subtraction………………………………………………………………………….. 24
4.3.2.13 Spectrum Multiplication………………………………………………………………………... 25
4.3.2.14 Spectrum Division……………………………………………………………………………… 26
4.3.3 Assistant Functions………………………………………………………………………………….. 27
4.3.3.1 Define Display Information……………………………………………………………………. 27
4.3.3.2 Edit Print Information…………………………………………………………………………... 27
4.4 Time Scanning…………………………………………………………………………………………….. 28
4.4.1 Scan Sample…………………………………………………………………………………………. 28
4.4.2 Graph Processing……………………………………………………………………………………. 29
4.4.3 Assistant Functions………………………………………………………………………………….. 29
 4.4.3.1 Calculate Rate………………………………………………………………………………….. 29
4.4.3.2 Define Display Information……………………………………………………………………. 29
4.4.3.3 Edit Print Information…………………………………………………………………………... 30
4.5 DNA/Protein Measurement………………………………………………………………………………. 30
4.5.1 DNA/Protein Measurement…………………………………………………………………………. 30
4.5.2 Assistant Functions………………………………………………………………………………….. 32

5. Instrument Validity………………………………………………………………………………………. 33
5.1 Validity Measurement…………………………………………………………………………………….. 33
5.1.1 Photometric Validity Measurement………………………………………………………………… 33
5.1.2 Wavelength Validity Measurement………………………………………………………………… 34
5.1.3 Assistant Functions………………. ………………………………………………………………… 36
5.1.3.1 Recalculate……………………………………………………………………………………... 36
5.1.3.2 Set Data Font…………………………………………………………………………………… 36
5.1.3.3 Edit Measurement Information……………………………………………………………….. 36
5.2 Energy Scan………………………………………………………………………………………………. 36
5.3 Spectrum 37
Slitwidth…………………………………………………………………………………………
5.4 View Dark Current………………………………………………………………………………………… 37

6. Assistant Functions…………………………………………………………………………………….. 38
6.1 Control the Instrument……………………………………………………………………………………. 38
6.1.1 Connect/Disconnect to 38
Spectrophotometer………………………………………………………..
6.1.2 Scan System Baseline………………………………………………………………………………. 38
6.1.3 Switch On/Off W Lamp……………………………………………………………………………… 38
6.1.4 Switch On/Off D2 Lamp……………………………………………………………………………... 38
6.1.5 Setting the Lamp Switching Wavelength Position………………………………………………... 38
6.1.6 Locate 656.1nm……………………………………………………………………………………… 39
6.1.7 Change Slitwidth……………………………………………………………………………………... 39
6.2 File Operation……………………………………………………………………………………………... 39
6.2.1 Save a File……………………………………………………………………………………………. 39
6.2.2 Load a File……………………………………………………………………………………………. 39
6.2.3 Open a File From Instrument……………………………………………………………………….. 39
6.3 Password Protection……………………………………………………………………………………… 40
6.3.1 Setting a Password………………………………………………………………………………….. 40
6.3.2 Changing a Password……………………………………………………………………………….. 40
6.4 Auto Sampling…………………………………………………………………………………………….. 40
 Functions
1. Functions
This section introduces the functions of the UV-Vis Analyst.

1.1 Main Functions

Single wavelength photometric measurement
 Go to a desired wavelength quickly and conveniently.
 Photometric value display mode can be changed (%Transmittance or Absorbance).

Fixed Points Measurement

Multi-wavelength Photometric Measurement
 Up to 20 wavelength points can be set up.
 Results will be grouped into a table format automatically.

Concentration Measurement
 2 methods to set up the regression curve.
Up to 20 standards to set up the regression curve. The UV-Vis Analyst will calculate the working
curve using a linear equation that fits the data. Enter factor values to generate regression curve.
 3 methods for curve fit.
Linear fit, Quadratic fit and Cubic fit.

Wavelength Scanning
 Allow user to set scan step (0.1, 0.2, 0.5, 1.0 and 5.0nm).
 Spectrum display mode can be changed (Wavelength-%Transmittance or Wavelength-Absorbance).
 Peaks and valleys will be automatically detected after scanning (User can define the peak threshold).
 Powerful spectrum processing functions are provided.

Time Scanning
 Allow user to set scan Interval (0.5, 1.0, 2.0, 5.0, 10, 30 and 60s).
 Spectrum display mode can be changed (Time-%Transmittance or Time-Absorbance).
 Peaks and valleys will be automatically detected after scanning (User can define the peak threshold).
 Powerful spectrum processing functions are provided.

DNA/Protein Measurement
 Wavelength points and ratios can be set up.
 Results will be grouped into a table format automatically.

1.2 Spectrum Processing Function

Trace a Spectrum
The cursor can be moved to a desired point in the spectrum displayed on the screen and the photometric
data at this point is displayed.

Automatic Peak Detection

After a scanning is complete, peaks and valleys can be automatically detected and listed in a table format.
They will also be labeled on the spectrum.

Scale Expansion
Simultaneous expansion of the X and Y axes are provided with the “Zoom” function. Display range can also
be changed though the “Display Setup” function.

Differentiation
You can calculate and display the first through to the fourth derivative spectrum for a given spectrum.
Derivative spectrum is useful for enhancing spectrum data that are not readily apparent in an absorbance
spectrum.

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 Functions
Calculate Spectrum
You can calculate addition, subtraction, multiplication and division between two spectrum with the resulting
data displayed on the screen.

1.3 System Check and Calibration Function

Instrument Validity Check
Up to 10 wavelength points can be set up in the instrument validity mode. Two methods can be selected
(Photometric Validity measurement and Wavelength Validity measurement) and tolerance can be entered.
Results will be grouped into a table format automatically.

Dark Current Check

You can resample the dark current of the instrument.

Spectrum Slitwidth Check

A special scan for checking spectrum slitwidth and it will calculate the spectrum slitwidth value
automatically.

Energy of Light Sources Check

It allows scan the energy of light sources with a fixed amplifier (0-10).

Reset Wavelength
It affords to relocate the 656.1nm.

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 Setup

2. Setup
This section introduces how to setup the UV-Vis Analyst to PC.

2.1 System Requirements for the UV-Vis Analyst

 486 or Pentium processor-based personal computer
 CD-ROM driver
 USB port
 16 MB of RAM (32 MB or more recommended)
 16 MB of available hard disk space
 Microsoft Windows 95, Windows 98/Me, Windows 2000 or Windows XP

2.2 Setup the UV-Vis Analyst to PC

1. Insert the CD-ROM of the UV-Vis Analyst into CD-ROM driver.
2. Open the directory of CD-ROM.
3. Double-click the icon Setup.exe to start to setup (Fig. 2-1). Click Next.
4. Input user’s information (Fig. 2-2). Click Next.

Fig. 2-1 Fig. 2-2

5. Select setup directory (Fig. 2-3). Click Next.

6. Select setup type (Fig. 2-4). Click Next.

Fig. 2-3 Fig. 2-4

7. Select program fold (Fig. 2-5). Click Next to copy files to PC (Fig. 2-6).

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 Setup

Fig. 2-5 Fig. 2-6

8. Click Finish to complete and exit setup (Fig. 2-7).

Fig. 2-7

2.3 Remove the UV-Vis Analyst from PC

Start→Control Panel→Add or Remove Programs→Select UV-Vis Analyst→Change/Remove.

2.4 Install the dongle

Plug the dongle into the USB port on PC (Fig. 2-8).

Fig. 2-8

2.5 Run the UV-Vis Analyst

There are two ways to start the UV-Vis Analyst:
1. Double-click shortcut icon on the desktop.
2. Start→All Program→UV-Vis Analyst→UV-Vis Analyst.

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 Setup

2.6 Set Comm. Port

Start the UV-Vis Analyst, on the UV-Photometer menu, click Comm. Port Setup appears the following box
(Fig. 2-9), select the Comm. Port (based connection of the USB cable) and Baud Rate (38400), click OK.

Fig. 2-9

The UV-Vis Analyst can not control the Instrument before plugged the dongle into USB
port and set the Comm. Port.

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Introduction
3. Introduction
This chapter introduces the interface of the UV-Vis Analyst.

3.1 Main Interface

After running the UV-Vis Analyst, the Main Form appears on the display (Fig. 3-1).

Fig. 3-1

3.2 Menu Bar and Toolbar

Menu bar and Toolbar are both provided in the software offering you two ways to select a desired function.
 On the menu bar, use your keypad or mouse to select the desired function.
 Almost all the functions listed in the menu bar can be reached by clicking a corresponding button in
the toolbar.

Main Menu Sub Menu Tool Function

New a Fixed Points Measurement

New a Wavelength Scan Measurement

File New
New a Time Scan Measurement

New a DNA/Protein Measurement

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Introduction

New a Instrument Validity

Open… Open a spectrum/data file

Close Close current measurement

Save Save current measurement

Save As… Save current measurement as a new file name

Open file from

Open a file saved in instrument
UV-Photometer

Export Export data or method

Print… Print test report

Print Setup… Setup printer

Exit Exit UV-Vis analyst

Status Bar Display/Hide status bar

Status of
Display status of spectrophotometer
Spectrophotometer

Status font Setup font of status bar

Customize Define the information of display and print

View
Peaks Mark peak value

Valleys Mark valley value

Magnify Magnify the area selected

Restore Restore the default parameters for display

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Introduction

Search Search peak/valley one by one

Link
Connect to the Instrument
Spectrophotometer
Reset
Reset parameters of instrument
Spectrophotometer

Escape Stop current measurement

View dark Current Retest the dark current

Set Amplifier Reset amplifier

Locate 656.1nm Relocate 656.1nm

Calibrate System
Scan system baseline
Baseline
Automatic Blank
UV-Photometer Do blank
Calibration

Slit Bandwidth * Set slit bandwidth (0.5, 1.0, 2.0, 4.0)

Set Unit Set unit

Turn on/off W lamp Turn on/off W lamp

Turn on/off D2 lamp Turn on/off D2 lamp

D2/W Switch Point Set switch point of D2/W

Comm. Port Setup Setup comm. port

Change Password Set/Change login password

Locate Cell ** Locate cell (1-8) to light path

Auto-sample
Setup Multicell ** Setup Multicell

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Introduction

Autorun ** Measure multi samples automatically

Start Start a measurement

Scan Stop Stop a measurement

Service Measure spectrum and scan energy

Display Range Setup scan display parameters

Settings
Peak Height Define peak/valley threshold

Add Add two spectrum

Sub Subtract one spectrum from another

Multiply Multiply two spectrum

Divide Divide one spectrum from another

Compute
Moving Window Smooth a spectrum with the method Moving Window
Averaging Averaging
Savitzky-Golay Smooth a spectrum with the method Savitzky-Golay
Smoothing Filter Smoothing Filter

Derivate Derivative of a spectrum

Resample Resample a spectrum

New Window New a measurement window as current

Cascade Multi windows display in a cascade

Window
Tile Multi windows display in a tile

Arrange Icons Arrange all icons minimized

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Introduction

Split Split display area

About UV-Vis
Help Display the information about the UV-Vis Analyst
Analyst

Setup measurement parameters

Modify a measurement result

Delete results selected

Set and Goto one wavelength

Display Instrument CPU information

Delete current Spectrum

Display result as mode %T

Display result as mode Abs

Undo Scale

“*” Only for variable bandwidth models.

“ ** ” These buttons only available when Auto cell holder is fitted.

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Operation
4. Operation
This chapter introduces the operation of the UV-Vis Analyst.

4.1 Single Wavelength Photometric Measurement

The UV-Vis Analyst provides a convenient method to measure photometric value at a fixed wavelength.

1. Click on the toolbar, appears Goto specified wavelength form (Fig. 4-1).

Fig. 4-1

2. Key in the desired wavelength position, click Goto. The minimum wavelength step is 0.1nm in a
range from 190-1100nm.
3. Place a reference in the sample compartment, click Zero.
4. Place a sample in the sample compartment. The wavelength position and photometric value will be
displayed in the Readout box.

4.2 Fixed Point Measurement

This UV-Vis Analyst performs fixed wavelength measurement at 1-20 points and how to analyze unknown
compounds against calibration standards.

4.2.1 Multi-wavelength Photometric Measurement

1. Click on the toolbar, appears follow form (Fig. 4-2).

Fig. 4-2

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Operation
2. Click the Method tab.
3. Type the number of wavelength points in the Number of WL Points box, or click the up/down
arrows next to the box set the wavelength points. Leave the two boxes Calculate Concentration
and Use Standard Samples.
4. Key in the wavelength in the Wavelength box.

5. Place a reference in the sample compartment. Click to do blank.

6. Click the Sample tab. It will display the following (Fig. 4-3). The control menu contains six buttons:
Start, Delete, Modify, Recalculate, Data Font and Print.

Fig. 4-3

7. Place a sample in the sample compartment. Click Start or to run a new measurement. The
display will change to the following (Fig. 4-4). Key in the sample name in the Name box.

Fig. 4-4

8. Click OK. The photometric data for sample will be listed in the Sample table.
9. Repeat steps 7-8 to measure all samples (Fig. 4-5).

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Operation

Fig. 4-5

4.2.2 Concentration Measurement

4.2.2.1 Set Up Linear Regression Curve
There are two methods available to set up the linear regression curve. You can use standards to set up the
regression curve or just key in the parameters manually. Use the following steps to select the method you
wish to use.

1. Click on the toolbar.

2. Click the Method tab.
3. Enter the number of wavelength points in the Number of Points box, or click the up/down arrow
next to this box. With 2 wavelengths, the absorbance at the second reference wavelength is
subtracted from the first to correct for background absorbance. With 3 wavelengths, the baseline
between the first and third wavelengths is calculated and its value at the second wavelength is
subtracted from the absorbance at the second wavelength to give the peak height.
4. Key in the wavelengths in the Wavelength boxes.
5. Tick the Calculate Concentration check box to activate concentration calculation.
6. Set up the linear regression curve.
Method 1: Set up the linear regression curve with prepared standards.
(1) Tick the Use Standard Samples check box.

(2) Place the reference into the sample holder. Click to do blank.
(3) Click the Standard tab.
(4) Place Standard 1 in the sample compartment. Click Start to run a measurement.
(5) Key in the concentration value of Standard 1 in the Conc. box.
(6) Key in the sample name for the standard in the Name box.
(7) Click OK. The photometric data, △A and concentration will be shown in the standard table.
(8) Repeat steps 4-7 to measure all the prepared standards (Fig. 4-6).

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Operation

Fig. 4-6

(9) Click down arrow in Curve Fit box to select curve fit method.
Method 2: Input the factor of the linear regression curve.
(1) Leave the Use Standard Samples check box.
(2) Click down arrow in Curve Fit box to select curve fit method.
(3) Input the factor of the linear regression curve.
7. Click Fitting tab to view the linear regression curve (Fig. 4-7). Click Display Setting tab to set the
display parameters and unit of concentration (Fig. 4-8).

Fig. 4-7

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Operation

Fig. 4-8

4.2.2.2 Measure Concentration by Using The Linear Regression Curve

The following procedure shows how to measure concentration of samples.

1. Set up linear regression curve (Refer 2.2.1) or click to open a file of linear regression curve
(*.QUA).

2. Place reference into the sample holder. Click to do blank.

3. Click the Sample tab.
4. Place Sample 1 into the sample holder.
5. Click Start to run a measurement.
6. UV-Vis Application Software will display the photometric value of Sample 1 at the fixed wavelength
positions automatically. Type the sample name in the Name box. The default is Sample-1.
7. Click OK. The photometric result for Sample-1 will be listed in the sample data. Delta Abs. and
concentration value of Sample-1 will also be displayed in columns 3 and 4.
8. Repeat steps 4-7 to measure remaining samples (Fig. 4-9).

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Operation
Fig. 4-9
4.2.3 Assistant Functions
The following procedure shows how to modify, delete and recalculate results.

4.2.3.1 Delete a Result

Click the Sample name label to select the result you want to delete, click or button Delete.

4.2.3.2 Modify a Result

Click the Sample name label to select the result you want to modify, click or button Modify.

4.2.3.3 Recalculate Concentration

If you change the linear regression curve, you need not remeasure the samples, click button Recalculate
to get new concentration values.

4.2.3.4 Set Data Font

Click button Data Font to set font of data table.

4.2.3.5 Edit Measurement Information

Click tab Information, type the information that will print out with measurement report.

4.3 Wavelength Scanning

This chapter describes how to collect a spectrum while using Wavelength Scan function.

4.3.1 Scan Sample

1. Click on the toolbar to new a sample scan measurement, appears the following form (Fig. 4-10).

Fig. 4-10

2. Click on the toolbar, appears the following form (Fig. 4-11). Input start wavelength in From box
(range: 190-1100nm), end wavelength in To box (range: 190-1100nm), select scan interval (0.1, 0.2,
0.5, 1.0, 2.0 or 5.0nm) and Filter times (1, 3, 5, 10 or 30), click OK.

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Operation

Fig. 4-11

3. Click on the toolbar to select %Transmittance mode or click to select Absorbance mode.

4. Click on the toolbar to set display parameters (Fig. 4-12).

Fig. 4-12

5. Place reference into the sample holder. Click to scan baseline.

6. Place sample into the sample holder. Click to scan sample, the real time spectrum will be

displayed (Fig. 4-13). Click to cancel while scanning.

Fig. 4-13

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Operation
4.3.2 Spectrum Processing
After you have acquired a spectrum, the spectrum processing options are available.

4.3.2.1 Auto List Peaks and Valleys

Click on the toolbar to set the peak/Valley threshold (range: 0 to 1.000, step: 0.001, Fig. 4-14), Input

the threshold value, click OK. Click to list peaks and click to list valleys (Fig. 4-15).

Fig. 4-14

Fig. 4-15

4.3.2.2 Rescale

Click on the toolbar to set the new parameters for display.

4.3.2.3 Original Scales

Click on the toolbar to restore the default display settings.

4.3.2.4 Zoom Selected Area

Click on the toolbar to activate zoom function. Position the cursor in the upper-left corner of the area
you want to select. Hold the left mouse button to drag the cursor to outline the spectrum area you want to
enlarge (Fig. 4-16). Release the mouse button. The part of the spectrum which is displayed within the

outlined area will be enlarged (Fig. 4-17). Click to undo scale. To cancel zoom to click again.

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Operation

Fig. 4-16

Fig. 4-17

4.3.2.5 Trace a Spectrum

Click on the toolbar, A crosshair cursor appears, move the cursor on the spectrum. Move the
crosshair cursor left or right on the spectrum. The data in the cursor window indicate the X-axis and Y-axis
values for the current cursor location (Fig. 4-18). Double click the left mouse button to release the crosshair
cursor.

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Operation

Fig. 4-18

4.3.2.6 Select a Spectrum as Current

As UV-Vis Application Software can display several spectrum overlaid on the screen, you should specify
the spectrum you wish to process. Click the down arrow on the toolbar (Fig. 4-19). All spectrums will be
listed in the pull-down menu. Click the spectrum you want to select. Its name will be listed in the Name Box
and it will be referred to as Current Spectrum.

Fig. 4-19

4.3.2.7 Derivative

Click on the toolbar. The following dialogue box appears (Fig. 4-20). Key in the class of derivative
(1-10, depending on whether 1st, 2nd, … 10th derivative is required) and type a name for the result
spectrum, then click OK. The result spectrum will be displayed overlaid with the original one (Fig. 4-21).

Fig. 4-20

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Operation

Fig. 4-21

4.3.2.8 Moving Window Averaging

Click on the toolbar. Appears following form (Fig. 4-22). Click up/down arrow of the Range box to
select range value, key a file name in the Name box, click OK. The result spectrum will be displayed
overlaid with the original one (Fig. 4-23).

Fig. 4-22

Fig. 4-23

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Operation
4.3.2.9 Savitzky-Golay Smoothing Filter
On the Computer menu, click Savitzky-Golay Smoothing Filter. Appears following form (Fig. 4-24). Click
up/down arrow to select the parameters, key a file name in the Name of Result box, click OK. The result
spectrum will be displayed overlaid with the original one (Fig. 4-25).

Fig. 4-24

Fig. 4-25

4.3.2.10 Resample

Click on the toolbar. The following dialogue box will be displayed (Fig. 4-26). Click Up/Down arrow
to select Sample times. Click OK. The new spectrum displays (Fig. 4-27).

Fig. 4-26

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Operation

Fig. 4-27

4.3.2.11 Spectrum Addition

Spectrum addition can assist in the development of artificial spectrum in multi-component mixtures.

Click on the toolbar. The following dialogue box will be displayed (Fig. 4-28). Click the down arrow
next to File 1 to select a spectrum and define it as source 1. Select a spectrum for File 2 in the same way. It
will not allow you to select the same spectrum twice. Key in a name for the Result spectrum and click OK.
The result spectrum will be displayed on the screen (Fig. 4-29).

UV-Vis Analyst will only add, subtract, multiply and divide two spectrums that are already
displayed on the screen. Before arithmetic processing, load or collect two spectrums from
memory.

Fig. 4-28

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Operation

Fig. 4-29

4.3.2.12 Spectrum Subtraction

Subtracting one spectrum from another has been a classical technique to offset spectrum interference from
the spectrum of interest.

Click on the toolbar. The following dialogue box will be displayed (Fig. 4-30). Click the down arrow
next to File 1 to select a spectrum and define it as source 1. Select a spectrum for File 2 in the same way. It
will not allow you to select the same spectrum twice. Key in a name for the Result spectrum and click OK.
The result spectrum will be displayed on the screen (Fig. 4-31).

Fig. 4-30

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Operation

Fig. 4-31

4.3.2.13 Spectrum Multiplication

Multiplying spectrum can assist in the development of artificial structure of spectrum in multi-component
mixtures.

Click on the toolbar. The following dialogue box will be displayed (Fig. 4-32). Click the down arrow
next to File 1 to select a spectrum and define it as source 1. Select a spectrum for File 2 in the same way. It
will not allow you to select the same spectrum twice. Key in a name for the Result spectrum and click OK.
The result spectrum will be displayed on the screen (Fig. 4-33).

Fig. 4-32

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Operation

Fig. 4-33

4.3.2.14 Spectrum Division

Dividing one spectrum from another has been a classical technique to offset spectrum interference from
the spectrum of interest.

Click on the toolbar. The following dialogue box will be displayed (Fig. 4-34). Click the down arrow
next to File 1 to select a spectrum and define it as source 1. Select a spectrum for File 2 in the same way. It
will not allow you to select the same spectrum twice. Key in a name for the Result spectrum and click OK.
The result spectrum will be displayed on the screen (Fig. 4-35).

Fig. 4-34

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Operation

Fig. 4-35

4.3.2.15 Unload a Spectrum

Select the spectrum you want to unload as the Current Spectrum, Click on the toolbar to remove
the spectrum from the display.

4.3.3 Assistant Functions

4.3.3.1 Define Display Information

Click on the toolbar, appears the Settings to display and print the spectra form, click the Legend
tab (Fig. 4-36), type the information for display.

Fig. 4-36
4.3.3.2 Edit Print Information

Click on the toolbar, appears the Settings to display and print the spectra form, click the Print tab
(Fig. 4-37), type the information for print out.

Fig. 4-37

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Operation
4.4 Time Scanning (Kinetic Analysis)
This chapter tells you how to obtain the absorbance or transmittance value for a sample as a function of
time at a given wavelength.

4.4.1 Scan Sample

1. Click on the toolbar, the following dialog box will appear (Fig. 4-38).

Fig. 4-38

2. Click on the toolbar to select the %transmittance mode or click to select the absorbance
mode.

3. Click on the toolbar. A dialogue box will be displayed (Fig. 4-39). Key in the wavelength, total
time (in seconds) and scan step in the above dialog box. The wavelength range should be within 190 to
1100 nm. The upper limit for total time is 100000 seconds. Seven scan intervals can be selected from
0.5S, 1S, 2S, 5S, 10S, 30S and 60S. Click OK.

Fig. 4-39

4. Place a reference in the sample holder. Click on the toolbar.

5. Take out the blank in the sample holder, place a sample in it and close the cover.

6. Place a sample in the sample holder. Click on the toolbar. The instrument will start scanning
automatically. The graph will be displayed on the screen during time scanning (Fig. 4-40). You can stop

scanning by clicking .

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Operation

Fig. 4-40

4.4.2 Graph Processing

Please refer 4.3.2.

4.4.3 Assistant Functions

4.4.3.1 Calculate Rate

Click on the toolbar, appears the Settings to display and print the spectra form, click the
Dynamic Analysis tab (Fig. 4-41), type the begin time in Time Begin box, type the end time in Time End
box, and type the K factor in K Factor box, click Calculate, the result will be displayed.

Fig. 4-41

4.4.3.2 Define Display Information

Click on the toolbar, appears the Settings to display and print the spectra form, click the Legend
tab (Fig. 4-42), type the information for display.

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Operation

Fig. 4-42

4.4.3.3 Edit Print Information

Click on the toolbar, appears the Settings to display and print the spectra form, click the Legend
tab (Fig. 4-43), type the information for display.

Fig. 4-43

4.5 DNA/Protein Measurement

This chapter describes how to perform DNA/Protein measurement.

4.5.1 DNA/Protein Measurement

1. Click on the toolbar, the following dialog box will appear (Fig. 4-44).

Fig. 4-44

2. Click the down arrows of the method to select the test method. Key in the wavelength position in the
Wavelength box. Key in the value of DNA/Protein Conc.

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Operation
3. Place a reference in the sample holder. Click on the toolbar to do blank.
4. Click the Sample tab. It will display the following (Fig. 4-45). The control menu contains six buttons:
Start, Delete, Modify, Recalculate, Font and Print.

Fig. 4-45

5. Place a sample in the sample holder. Click Start or to run a new measurement. The display will
change to the following (Fig. 4-46).

Fig. 4-46

6. The UV-Vis Analyst will read the photometric value of sample 1 at the fixed wavelength automatically.
Key in the sample name in the Name box. Click OK after the measurement is complete. The
photometric data for sample 1 will be listed in the sample table.
7. Repeat steps 5-6 to test all samples (Fig. 4-47).

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Operation

Fig. 4-47

4.5.2 Assistant Functions

Please refer 4.2.3.

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 Instrument
Validity
5. Instrument Validity
This chapter describes how to perform Instrument Validity.

5.1 Validity Measurement

5.1.1 Photometric Validity Measurement

1. Click on the toolbar. The following form appears (Fig. 5-1).

Fig. 5-1

2. Click the down arrows of the method to select Photometric Validity Test.
3. Type the number of wavelength points in the Number of Points box, or click the up/down arrows next
to the box set the wavelength points. Key in the wavelength position in the Wavelength box and key in
the standard value in the Standard box. Key in the tolerance in the Parameters box.

4. Place a blank or air in sample holder. Click on the toolbar to do blank.

5. Click the Sample tab. Appears following form (Fig. 5-2).

Fig. 5-2

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Validity
6. Place the Photometric Standard Filter in sample holder, click to run a new measurement,
appears following form (Fig. 5-3). Click OK to list the data in the table (Fig. 5-4).

Fig. 5-3

Fig. 5-4

5.1.2 Wavelength Validity Measurement

1. Click on the toolbar. The following form appears (Fig. 5-5).

Fig. 5-5

2. Click the down arrows of the method to select Wavelength Validity Test.
3. Type the number of wavelength points in the Number of Points box, or click the up/down arrows next

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 Instrument
Validity
to the box set the wavelength points. Key in the wavelength position in the Wavelength box. Key in the
tolerance in the Parameters box.

4. Place a blank or air in sample holder. Click on the toolbar to do blank.

5. Click the Sample tab. Appears following form (Fig. 5-6).

Fig. 5-6

6. Place the Wavelength Standard Filter in sample holder, click to run a new measurement,
appears following form (Fig. 5-7). Click OK to list the data in the table (Fig. 5-8).

Fig. 5-7

Fig. 5-8

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Validity
5.1.3 Assistant Functions
5.1.3.1 Recalculate
If you change the parameters, you need not remeasure the samples, click button Recalculate to get new
values.

5.1.3.2 Set Data Font

Click button Data Font to set font of data table.

5.1.3.3 Edit Measurement Information

Click tab Information, type the information that will print out with measurement report.

5.2 Energy Scan

1. Click on the toolbar to new a sample scan measurement.

2. Click to select Absorbance mode.

3. Click on the toolbar to set display parameters (Xmin=200, Xmax=1000,Ymin=0,Ymax=6).

4. Click Scan → Service → Energy Scan on the Menu, appears following form (Fig. 5-9), select the

amplifier, click OK to scan (Fig. 5-10). Click to cancel while scanning.

Fig. 5-9

Fig. 5-10

5. The value of every point multiply 10000 is the energy value.

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Validity
5.3 Spectrum Slitwidth
1. Click on the toolbar to new a sample scan measurement.

2. Click to select Absorbance mode.

3. Click on the toolbar to set display parameters (Xmin.=645, Xmax.=665,Ymin.=0,Ymax.=0.5).

4. Click Scan → Service → Spectrum Slitwidth on the Menu, it will scan from 665nm to 645nm. Click

, the peak and the spectrum slitwidth value list in the data table (Fig. 5-11).

Fig. 5-11

5.4 View Dark Current

Click UV-Photometer→View Dark Current on the Menu, it will appears following form (Fig. 5-12), and the
dark current value will list in the table.

Fig. 5-12

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Functions
6. Assistant Function
6.1 Control the Instrument
6.1.1 Connect / Disconnect to Spectrophotometer

Click to connect to spectrophotometer, and appears the following form (Fig. 6-1) if connected
successfully. Click again to disconnect.

Fig. 6-1
6.1.2 Scan System Baseline

Click to scan a system baseline.

6.1.3 Switch On/Off W Lamp

Click to switch off the W lamp, click it again to switch on.

You must warm up the W lamp about 10 minutes before measure samples.

6.1.4 Switch On/Off D2 Lamp

Click to switch off the D2 lamp, click it again to switch on.

You must warm up the D2 lamp for about 20 minutes before measure samples.

6.1.5 Setting the Lamp Switching Wavelength Position

Click UV-Photometer→D2/W Switch Point on the menu, appears following form (Fig. 6-2), Key in the
lamp switching wavelength position in the New point box. It should be within the range 339 nm to 370 nm.
Click Setup return to the wavelength scan sub-menu.

Fig. 6-2

If the switching point of the lamps is changed, a new baseline correction must be
performed.

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Functions
6.1.6 Locate 656.1nm
Keep the light clear. Click UV-Photometer→Locate 656.1nm on the menu, the Spectrophotometer will
search the 656.1nm.

6.1.7 Change Slitwidth (Only for Variable Bandwidth Models)

Click UV-Photometer→Change Slitwidth on the Menu, then select the slitwidth (0.5nm, 1.0nm, 2.0nm
or 4.0nm).

6.2 File Operation

6.2.1 Save a File

Click , a new dialog box will be displayed as follow (Fig. 6-3). Type in a file name, click Save.

Fig. 6-3
6.2.2 Load a File

Click , the display will change to the following (Fig. 6-4). Select a folder and filename. Click Open to
open the selected file.

Fig. 6-4
6.2.3 Open a File From Instrument

Click , the display will change to the following (Fig. 6-5). Select a file type and filename. Click Open
to open the selected file.

Fig. 6-5

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 Assistant
Functions

6.3 Password Protection

6.3.1 Setting a Password
Click UV-Photometer→Change Password on the menu. The following prompt appears (Fig. 6-6). Enter
up to 8 characters in the New Password field. Re-enter exactly the same characters in the Confirm it field.

Fig. 6-6

Any characters can be used, but the password is case-sensitive. Ensure you use the same
case when entering characters in both fields. If exactly the same characters are not
entered in both fields, you will be prompted to try again. If you wish to abort setting a
password, clear both fields by deleting all characters there in. Once a password is selected,
the next time you start the UV-Vis Analyst, the following prompt will appear (Fig. 6-7) Input
your password and click Login.

Fig. 6-7

6.3.2 Changing a Password

Once a password has been set, the New Password and Confirm it fields are greyed out although the
Change Password field is active. To change the current password，Type the current password in the Old
Password field. Only if the old password is correct will the New Password and Confirm it fields become
active. Proceed as per “Setting a New Password” and enter the new password in both the New Password
and Confirm it fields.

6.4 Auto sampling (Needs 8-Cell Automatic Cell Changer)

Click on the toolbar, the following prompt will appear (Fig. 6-8). Tick the numbers of the cells and key

the name in the Name box. Click OK. Click on the toolbar, it will complete measuring automatically.

Fig. 6-8

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Functions

Drawell International Technology Limited

Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1117,Lane561 XiuChuan Rd., Greeland Max-Mall,PuDong,Shanghai
Tel: 0086 21 54411195
Fax: 0086 21 33823261
Web : www.drawell.com.cn
Email : [email protected]

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