RECYCLING PREPARATIVE HPLC

LC-92XX NEXT SERIES

USER'S MANUAL

Future of Polymer Science is it.

Japan Analytical Industry Co., Ltd.

URL http: //www.jai.co.jp/ E-mail: [email protected]

□M ai n O f f ic e： 〒190-1213 208 Musashi, Mizuho, Nishitama, Tokyo TEL: 042-557-2331 FAX: 042-557-1892
□Osaka Branch： 〒532-0002 5-13-8-303 Higashimikuni, Yodogawaku, Osaka city TEL: 06-6393-8511 FAX: 06-6393-8525
□Nagoya Branch： 〒465-0025 3-609-3D Kamiyashiro, Meitoku, Nagoya city TEL: 052-709-5400 FAX: 052-709-5403
 RECYCLING PREPARATIV E HPLC

LC-92XX NEXT SERIES

USER'S MANUAL
Preface
Thank you for introducing Recycling Liquid Chromatograph model LC-92XX NEXT.
This manual contains instruction of the LC-92XX NEXT's operational manual,
technical information and safety information.
Refer the manual thoroughly, for applying LC-92XX NEXT series to your research
and investigation with safety operation.
Keep the manual near by the LC-92XX NEXT Liquid Chromatograph for referring
any time.

Printing information
・No part of this document may be photocopied or reproduced, or translated to
another program language without the prior written consent of Japan Analytical
Industry Co., Ltd.
・Please note that the information contain in this document may be revised without
notice.
・Please contact our representative office or our company to make inquiries about
the information contained in this document.
・Manuals with missing pages or incorrect collating will be replaced.
Warranty Statement
JAI warrants the Recycling Preparative HPLC LC-92XX NEXT series as follows,
only when you operate it correctly following the instructions in this manual and
without remodeling it.

Warranty period

LC-92XX NEXT Products are warranted for a period of one year from the
date of installation.

Warranty detail

If the product is found to be defective due to a manufacturing fault during

the warranty period, JAI will repair or replace the product or defective
parts without charge.
Negative assurance

Even during the warranty period, the defects caused by the following are
not covered by this warranty:

・Improper use.
・Operations under the condition not authorized in this document or our
company.
・Abnormal power supply or use under the conditions of irregular voltage
or frequency.
・Defect is coming from other factors, not from the LC-92XX NEXT.
・Use under severe environmental conditions such as corrosive gas, high
temperature and humidity, or continuous vibration.
・Flowing a highly corrosive liquid.
・Force majeure such as earthquake, fire, or lightning.
・Removal or transfer of the product after installation.
・Remodeling or Disassembly of the LC-92XX NEXT with not written way
in this manual.
・Using an instrument that is resold without our approval.
・Defects of the consumables or the parts of which the warranty period is
limited are not warranted.

Parts of consumption ：
Pump seal, pump plunger, pump plunger guide, pump check valve, line filter, manual
sample injector (rotor seal/stator face), selector valve (solvent selector, recycle flow 1,
recycle flow 2, drain, C/D selector)

The items which are not included in this paragraph are out of the
warranty.
Direct or indirect damages coming from the evident or implied warranties
of this instrument are not compensated.
Direct or indirect damages coming from destroy of the instrument are not
compensated.
How to Use This Document
This document "LC-92XX NEXT SERIES USER'S MANUAL" consists of the following
four volumes.
Choose the volume to use as needed.
Each volume has its own table of contents at the beginning.

Tab Daily General Installa- Mainte-

Operati- Inform- tion nance
on ation Manual Manual
Manual Manual
•Daily
operations •Installati- •Maintena-
• Con-
•Functions on nce
tent
•Informati- or other
on for new basic •Relocation •Trouble
users informati- shooting
on
To Operate in Safety
LC-92XX NEXT series instrument is a recycling preparative liquid chromatograph
to apply the separation analysis and separation collection of the samples by using
the principle of liquid chromatography.
Please read and protect to precaution for safely operations of the LC-92XX NEXT
series.

Cautions

・Do not use the LC-92XX NEXT series instrument other than a liquid
chromatography.
・Please read and adhere the instruction in this manual.
・Take safety measures to prevent an accident.
・Please note that the LC-92XX NEXT products that are remodeled or
disassembled without our prior notice are not warranted.
・In long term stop of the LC-92XX NEXT, disconnect Power cable. Seal column
ends with correct plugs to prevent column drying. Fill tubing with noncorrosive
solvent to prevent collision of tubing and flow line assemblies, but not flow the
solvent into the column.
・To avoid possible eye injury and skin trouble, wear eye protector and gloves.
・Prevent solvent leakage in the instrument.
・Do adequate ventilation in LC-92XX setting laboratory.
Support

Support on our Web site

Latest applications and soft ware revisions put on the site. News of our exhibition
and Events also put on the site.

We are awaiting your question, conference and request information of our

instrument.

Our Web site

http://www.jai.co.jp/
Emergency Measure
In emergency situation of the instrument or in disaster, do following emergency
measure.

Restart should be carefully to check the instrument performance. If you have any
worry, contact us or our representative.

Emergency stop

・Turn POWER SWITCH off to stop the LC-92XX NEXT.

・Turn off every POWER SWITCH of peripheral instruments.
・Avoid solvent spill and leakage.
・Cut off the power supply.
 VOLUME

Volume 1 Daily Operation Manual

Daily operations and information for new users
Contents

Volume 1 Daily Operation Manual

Chapter 1 Operation Flow ................................................ 1-1
Operation Panel Display ........................................................ 1-2
Operation Flow ....................................................................... 1-3
Chapter 2 General Operation ........................................... 1-4
Prepare the solvent, waste reservoir and sample. ................. 1-5
Turn the power switch on. ...................................................... 1-6
Press the [CLEAN UP] key..................................................... 1-6
Set parameters....................................................................... 1-7
Pump flow rate setting ................................................................. 1-7
Pump pressure limit setting .......................................................... 1-7
Detector setting ........................................................................... 1-8
Turn on the pump. .................................................................. 1-9
Turn on the integrator or recorder. ......................................... 1-9
Check the column stability. .................................................... 1-9
Inject the sample. ................................................................. 1-10
Separate recycles. ............................................................... 1-11
Collect the fractions.............................................................. 1-12
Shut down the LC................................................................. 1-13
Preface

This manual includes basic operation of the liquid chromatograph model LC-92XX
NEXT series to control.

For more details of information, installation, and maintenance of the instrument, please
refer to other volumes, "General Information Manual", "Installation Manual", and
"Maintenance Manual" on the tabs.
Cautions for Operation

・If a failure or wrong operation occurs in the LC-92XX NEXT, an error message
appears on the LCD display. At the beginning of the error message, "ERROR" is
displayed followed by a number. Based on this number, refer to a section in
"Volume 4 Maintenance Manual" to resolve the cause of failure.

・If you press the [Co/Dr] key for collecting fractions from a fraction nozzle during
the [RECYCLE] operation, an error message appears and fractions cannot be
collected. Stop the [RECYCLE] operation, and then press the [Co/Dr] key in
[FRACTION] to collect fractions.

Similarly, if you want to execute the [RECYCLE] operation during the

[FRACTION] operation, stop [FRACTION] before executing [RECYCLE] by
pressing the [Co/Dr] key in [FRACTION].

・For the keys located on the sheet switches other than [PURGE] and [AUTO
ZERO], you can press a key to execute its function, and press the key again to
cancel the function.
 1-1

Chapter 1 Operation Flow

Operation flow of LC-92XX NEXT series

1-1
Operation Panel Display

This section describes the overview of the operation panel of the LC-92XX NEXT
series.

LCD Display Arrow Keys

Displays numerical values such as Keys for changing the setting
the pump flow rate, pressure, and UV or RI values of the unit.
signal. [OK] Key

Logo and Product Name [CLEAN UP] key

1-2
Operation Flow
Actual procedure is as follows.

Prepare the solvent, waste reservoir and sample.

Turn the power switch on.

Press the [CLEAN UP] key.

Set parameters.

Turn on the pump.

Turn on the integrator or recorder.

Check the column stability.

Inject the sample.

Recycle separation.

Collect the fractions.

Shut down the LC.

1-3
 1-2

Chapter 2 General Operation

General operation flow of LC-92XX NEXT series

1-4
Prepare the solvent, waste reservoir and sample.

Solvent

・Prepare sufficient volume solvent in a container.

・Place the solvent container in the LC-92XX solvent stage.

・Prepare the solvent purer than chemical grade reagent. If Chloroform (CHCL3) for
the solvent is recommended, CHCL3 added ethanol for stabilizer is suitable.(Pure
CHCL3 or CHCL3 added Amylene stabilizer is not recommended.)

・In case of using mix solvent, mix each solvent completely.

・In case of using mix solvent, solvent mixture should be degassed perfectly. For
details of degassing, refer to "Appendix" P.2-48 in "General Information Manual".

・For the properties of typical solvent, refer to Appendix P.2-51 in "General Information
Manual".

・Filter the sample to remove micro particles with a suitable membrane. For details of
solvent's filtration, refer to Appendix P.2-53 in "General Information Manual".

・Be careful to handle the solvent and waste solvent.

・Prevent solvent leakage in the instrument.

・Do adequate ventilation in LC-92XX setting laboratory.

Waste
・A waste reservoir volume should be more than the solvent container. Reservoir
should be chemical proof against the solvent.

・Do not the reservoir to turn over and spill solvent.

Samples
・Sample should be filtered to remove particles before injection. For the recommended
sample filtration, refer to Appendix P.2-54 in "General Information Manual".

1-5
Turn the power switch on.

・Make sure that the receptacle is 100 VAC and enough to supply power for the LC-
92XX. Turn power switch on.

Power Switch

・After 15 seconds from turning the power switch on, the computer in the LC-92XX
boots up to indicate the initial stage.

Press the [CLEAN UP] key.

・Press the [START] key of [CLEAN UP] at the lower right side of the operation panel.

・When the manual operation is required, a message appears on the LCD display with
an intermittent beep.

・Follow the instructions displayed on the LCD display.

・After the completion of the [CLEAN UP] operation, turn the Column reject valve back
to its original position ([CONNECT]).

*The [CLEAN UP] operation needs 10 to 15 minutes.

*While the pump is in operation, [CLEAN UP] cannot be executed. Execute the operation when the pump
is stopped.

1-6
Set parameters.

Pump flow rate setting

・Press the [FLOW SET] key in [PUMP].

・The parameter to be changed blinks on the LCD display.

・Use the arrow keys to set the flow rate value to use.

・Press the [OK] key.

*While the pump is in operation, the value cannot be changed.

Perform the operation while the pump is stopped.

Arrow Keys

Pump pressure limit setting

・Press the [MAXIMUM SET] key in [PUMP].

・The parameter to be changed blinks on the LCD

display.

・Use the arrow keys to set the maximum pressure

(limit).

・Press the [OK] key.

・Similarly, press the [MINIMUM SET] key in the

[PUMP] unit tab to enter the minimum pressure.
*The values can be changed while the pump is in operation. Set the
values based on the Column pressure.
*The value "0" makes off pressure limit.
*Make sure the [MAXIMUM] value is more than the [MINIMUM]
value.

1-7
Detector setting
For UV DETECTOR

・When using the recorder, press the [RANGE]

key and adjust the value by using the or
arrow key.

・Normally use the RI detector with the [TEMP.C]

For RI DETECTOR key turned ON (LED is turned on).

・After the [CLEAN UP] operation, the [AUTO

ZERO] operation is not yet executed. After the
column equilibrium, press both the [AUTO
ZERO] keys in the [UV DETECTOR] and [RI
DETECTOR] unit tabs.

1-8
Turn on the pump.

・Press the [ON/OFF] key in [PUMP].

Turn on the integrator or recorder.

・Turn on the INTEGRATOR or RECORDER and monitor the detector signal. Make
sure to stabilize the base line of the detector signal.

・The INTEGRATOR or RECORDER is an optional product. Refer to each manual for

correct operation.

Check the column stability.

・In case an RI detector is attached, make sure that the base line of the RI detector
signal is stable.
・JAIGEL-H series (20φ x 600 mm), W series(20φ x 500 mm), GS series (20φ x 500
mm) the stability for each column, needs from 30 to 50 minutes. ODS and SIL
column should be waited with monitoring of the detector signal.
*In case of using the W and GS column, change of the solvent composition may need long time.

*Before the sample injection do zero adjustment referring to P.1-8.

1-9
Inject the sample.

・Prepare filtered sample. (For the typical filtering method, refer to "Micro
Particle Elimination in Sample Solution", Appendix P.2-54 in "Volume 2
General Information Manual").

・Suck the sample solution in a syringe to pull a plunger.

・Wipe the drop out of the syringe needle with clean paper.

・Hold the syringe upwardly, push the plunger gently to purge air from the
syringe. Push the plunger until the air is purged thoroughly.

・Insert the syringe into the manual injector thoroughly until the syringe needle
touches the bottom.

・Turn the handle of the [MANUAL INJECTOR] to the [LOAD] position.

・Push the plunger thoroughly.

・Turn back the handle to the [INJECT] position and take the syringe.

・Clean the needle port with clean solvent. (For details on how to clean the
needle port, refer to "Needle Port Cleaning", Appendix P.2-54 in "Volume 2
General Information Manual").

1-10
Separate recycles.

・Monitor the chromatograph to wait a target peak

appearing.

・When the peak is rising, press in [RECYCLE].

*The LED at the top of the key is turned on.

・At the end of the peak, press .

*The LED at the top of the key is turned off.

・When the peak is rising again, press in

[RECYCLE].

・When you get an effective separation, press to

stop the recycle operation.
*Between the recycle operations, the handle of the manual sample injector should be located at
the [LOAD] position.
If the injector located at the [INJECT] position, the manual sample injector makes dead
volume to diffuse the peaks on the separation of the recycling.

1-11
Collect the fractions.

Siphon box (at lower left side front of the

instrument)

Siphon

*Lamp turned off = Eluted from the siphon

*Lamp turned on = Eluted from the fraction nozzle

Fraction nozzle

・Monitor the chromatograph to wait a target peak

appearing.
*Normally the drain flows from the siphon.

・Press in [FRACTION].

・The LED at the top of the key blinks.

*The bar shows that a fraction of the sample is running dead volume
from the detector to the fraction nozzle.
*After running through the dead volume, the fraction flows from the
fraction nozzle.

・Press at the end of the peak.

*The fraction flows from the fraction nozzle until there is no fraction
that is running in the dead volume.

・Repeat the same operation for all the target peaks.

1-12
Shut down the LC.

・After collecting fractions, press the [ON/OFF] key in

[PUMP] to stop the pump.

・Press the [START] key of [CLEAN UP] at the lower right side of the operation panel.

・When the manual operation is required, a message appears on the LCD display with
an intermittent beep.

・Follow the instructions displayed on the LCD display.

・After the [CLEAN UP] operation, move the reject valve back to its original position to
complete the [CLEAN UP] operation. If the LC-92XX NEXT will not be used for a long
time, stay the valve position at [REJECT].

*If the column stays a long time in the stopped flow line, solvent may be lost by gravity to dry the column
packing and to damage the column.

・Turn off the integrator, recorder or other attachment.

・Turn off the LC-92XX NEXT.

1-13
 VOLUME

Volume 2 General Information Manual

General information of LC-92XX NEXT series (except optional accessories)
Contents

Volume 2 General Information Manual

Chapter 1 Description of LC-92XX NEXT ........................ 2-1
Composition of LC-92XX NEXT Series .................................. 2-2
Name and Function of Each Part ........................................... 2-3
Specification ........................................................................... 2-8
NEXT Series Features ........................................................... 2-9
Recycling preparative HPLC ........................................................2-9
Layout..........................................................................................2-9
Compact sized body.....................................................................2-9
Easy to operate............................................................................2-9
Auto-cleaning ............................................................................. 2-10
Non-diffusion function ................................................................ 2-10
Flow Channel Map ............................................................... 2-11
Operation Panel Display ...................................................... 2-14
Chapter 2 Description of Operation Panel ..................... 2-15
WAKE UP ............................................................................ 2-16
Description of Arrow Keys and [OK] Key.............................. 2-17
Description of Units .............................................................. 2-18
PUMP Unit ................................................................................. 2-18
Detector Unit .............................................................................. 2-21
UV Unit ...................................................................................... 2-21
RI Unit ....................................................................................... 2-24
FRACTION Unit ......................................................................... 2-26
RECYCLE Unit .......................................................................... 2-27
CLEAN UP ........................................................................... 2-28
LCD display during CLEAN UP operation ................................... 2-28
Chapter 3 Recycling Separation .................................... 2-31
Recycling Separation ........................................................... 2-32
Relation between Peak Separation and the number of
Recycling ................................................................................... 2-32
Note of good Recycling Separation ............................................ 2-34
For good Recycling efficiency..................................................... 2-37
Chapter 4 Condition Setting ........................................... 2-39
 Setting Operation ................................................................. 2-40
Pump setting.............................................................................. 2-40
Setting UV detector .................................................................... 2-42
Setting RI detector ..................................................................... 2-44
Setting fraction ........................................................................... 2-46
Chapter 5 Appendix........................................................ 2-47
Degassing.................................................................................. 2-48
Property of typical organic solvent .............................................. 2-51
Micro particle elimination ............................................................ 2-53
Needle port cleaning .................................................................. 2-54
 2-1

Chapter 1 Description of LC-92XX NEXT

LC-92XX NEXT compositions, operation flow-chart, and operation panel
functions

2-1
Composition of LC-92XX NEXT Series

The LC-92XX NEXT series is a high performance recycling preparative liquid

chromatograph for separation and purification.

The main body, pump, sample injector, column*, detector*, and integrator*
are installed as standard equipment.

High Resolution Hydrophobic SEC Column, High Resolution GFC

Column, High Resolution SEC Column, Optical Isomer separation
Column, Various Polymer Column, Partition and Adsorption Column
NEXT series can accommodate different separation method for both
analytical and preparative scale.
NEXT series UV detector and RI detector can be fit into the body together.

*Columns, Detectors, integrator, and recorder are optional accessories. Please select
optional accessories according to your purpose.

For details on using accessories, refer to separate instruction manuals for correct
operation.

2-2
Name and Function of Each Part

＜Front of the LC-92XX NEXT＞

Operation Panel Manual Injector Valve

Column Reject Valve

Siphon Box Pump Drain Valve

Name Function
To press the buttons to set conditions or execute the
Operation Panel
operation.
Manual Injector Valve To inject the sample manually.
Column Reject Valve To disconnect the flow line from the column.
Pump Drain Valve To drain the solvent from the pump outlet.
To collect fractions from the fraction nozzle or the
Siphon Box
siphon, or to collect the waste liquid.

2-3
<Inside of the LC-92XX NEXT Front Door>

Solvent Table

Pump Head

UV Detector

Power Switch

RI Detector

Wave Length

Tuner

Inside of

the Siphon

Box

Front Door

Name Function
Device using suction and pressure to force solvent
Pump Head
into the flow line.
Solvent Table A table to place a solvent reservoir.
Detector for absorption of the light after passing
UV Detector*1
through the UV cell.
Power Switch To turn on/off the power of the UV detector.
Wave Length Tuner*2 A tuner for wavelength range.
*1
RI Detector Detector for refractive index.
To collect fractions from the fraction nozzle or the
Siphon Box
siphon, or to collect the waste liquid.
To protect inner units from damages and leaking
Front Door
solvent.
*1 UV detector and RI detector are sold separately.
*2 Wave-length tuner is only available for variable wave length type UV detector.

2-4
 <Inside of the LC-92XX NEXT Right Side Column Cover>

Column Cover Pre-Column Holder

Manual Injector Sample Loop

Column Holder

TO COLUMN Connector

FROM COLUMN Connector

Null Glass Adj. Hole Cut Filter Exchanger

Name Function
Manual Injector A place to store sample temporarily right after
Sample Loop syringe injection.
Connector of solvent's flow line from the body
TO COLUMN Connector
to columns.
FROM COLUMN Connector of solvent's flow line from the
Connector columns to the body.
Pre-Column Holder To hold the Pre-Column.
Column Holder To hold the Column.
A slit for switching UV wave length range, in
Cut Filter Exchanger
case of installed UV-600 NEXT*1.
A hole for turning optical null glass to adjust the
Null Glass Adj. Hole optical zero point with screwdriver, in case of
installed RI-700 NEXT*1.
*1 UV detector and RI detector are sold separately.

2-5
<LC-92XX NEXT Left Side>
Output Port

Power Switch

*1
Solvent Out

Name Function
Power Switch To turn on/off the LC-92XX.
To output recognized signals from detectors
Output Port
and valves.
Solvent out being connected to the fraction
Solvent Out*1
collector. (Option)
*1 The fraction collector is an optional product. Normally it is not used.

2-6
 <Inside of the LC-92XX NEXT Siphon Box Door>
Siphon

Pump Drain Valve

Fraction Nozzle

Siphon Box Door

Manual Sample Injector Drain Out

Pump Drain Out

Name Function

Siphon Outlet of the eluent flowing from the detector.

Nozzle for collecting eluent fractions from the

Fraction Nozzle
detector.
Drain out pipe for solvent from pump drain
Pump Drain Out
valve.
To drain surplus solvent out when to inject
Manual Sample Injector
sample into manual injector or to clean needle
Drain Out
port.

2-7
Specification

If the explanation or specification is the same for both LC-9210 NEXT and LC-9225 NEXT, it is written
as 92XX NEXT in this manual.

LC-9210 NEXT LC-9225 NEXT

Pump Model FX-10 FX-25
Pump Type Reciprocating double plunger
Maximum Discharge
25 MPa 20 MPa*
Pressure
Flow Rate 0.1 to 10.0 mL/min 0.1 to 25.0 mL/min
Operation Panel 8.4-inch LCD operation panel
Manual Sample Injector Rheodyne 7725i
Sample Loop (Standard) 3 mL 10 mL
Event Marker Injection, recycle and collect
Recycle System Built-in
Non-diffusion Function Twin valve method
Auto-Cleaning Route washing by program
Dimension of Mainframe 464(W) x 492(H) x 504(D)
Weight Aprox. 38 Kg

*In the LC-9225 NEXT, the maximum discharge pressure differs depending on the flow rate to be
used.

Flow Rate (mL/min) Maximum Discharge Pressure (MPa)

0.1 to 9.9 20
10.0 to 19.9 15
20.0 to 25.0 10

2-8
NEXT Series Features
This section describes the functional characteristics of LC-92XX NEXT
series.

Recycling preparative HPLC

This is a liquid chromatography for separation and purification.

"Recycling function" enables the same solvent and the sample to
flow through columns more than one time to complete perfect
separation as if using "simulated" infinite columns.

Layout

The manual sample injector is located outside of the body, which

reduces time for tasks such as seal exchange and improves the
maintenance. The pump and detectors can be taken out from the
front of the body more easily.

Compact sized body

Body size made 1/3 smaller compared to our previous models with
improved functions and performance.

Easy to operate

A 8.4-inch LCD operation panel is installed. The keys on the

operation panel are designed for easy intuitive operation.

2-9
Auto-cleaning

Automated flow line cleaning function keeps flow channels clean

and solvent changeover very easy.

Non-diffusion function

During extended recycling period, the recycled sample in the loop

can diffuse back into the solvent line with the danger of
contaminating the solvent reservoir. The NEXT series incorporates
a patent pending non-diffusion device that prevents the sample
from backing into the solvent line. Thus, this keeps the lines of
NEXT series constantly clean automatically.

2-10
Flow Channel Map
The flow channel map of the LC-92XX NEXT series is as follows.

Valves and flow lines of LC-92XX NEXT

Solvent Manual Injector Valve

Column Reject Valve

Buffer Loading Valve

Pump

Buffer Volume

Pump Drain Valve

Recycle Valve 2 Recycle Valve 1

Collect Valve
Solvent Filter Detector

Buffer Solvent Drain

Column

Buffer Solvent Drain Siphon Fraction Nozzle

2-11
<Normal Flow Channel via Siphon>

<Normal Flow Channel via Fraction Nozzle>

2-12
<Recycle Flow Channel>

2-13
Operation Panel Display
This section provides the overview of the operation panel of the LC-92XX
NEXT series.

LCD Display Arrow Keys

The setting values and monitored values Keys for changing the setting [OK] Key
are displayed. values of units.

Unit Tab
The unit tabs and their

operation keys

Logo and Product [CLEAN UP] Key

Name

2-14
 2-2

Chapter 2 Description of Operation Panel

Description of the LC-92XX NEXT series operation panel

2-15
WAKE UP
WAKE UP is the state when the power is turned on.

It refers to the initial screen of the LCD display that starts when the power
switch is turned on.

The initial screen changes to the normal screen after approximately 15

seconds from power on.

<Example: WAKE UP of LC-9210 NEXT>

Power on

Initial screen 1

HE L LO ! H E L L O !
LC-NEXT SER I ES L C - N E X T

( C ) JA I ( C )

Initial screen 2

HE L LO ! HE L LO !
LC-NEXT SER I ES LC- NEX
LC- 9 2 1 0
( C ) JA I (C

HE L LO
LC- NE
Normal screen Flow rate
(
F L OW 09 . 9 0 m l ／m i n
Monitoring pressure
PRESS 0 . 0 MP a
UV 00 0 0 UV output
RI 00 0 0
RI output
*The LC-9210 NEXT model is used as example.
*While the initial screens 1 and 2 are displayed, the system performs the initialization,
verification of the previous settings, and preparation.
*Key operations are not available on the initial screen 1 or 2.

2-16
Description of Arrow Keys and [OK] Key

<How to Operate Arrow Keys>

: Increment a numerical value

: Decrement a numerical value

: Shift the blinking cursor to the right

: Shift the blinking cursor to the left

The arrow keys are mainly used to change various setting values.
The up arrow key increments the numerical value at the blinking
cursor, and the down arrow key decrements it. The right arrow key
shifts the blinking cursor to the right, and the left arrow key shifts it
to the left. After setting, press the [OK] key to update the setting
value.

<How to Operate [OK] Key>

This key is used to update the setting values. After setting a

numerical value, be sure to press the [OK] key.

*When the manual [CLEAN UP] operation is required or when an error message is displayed,
the LED blinks at the top of the key.

2-17
Description of Units

PUMP Unit

・In the [PUMP] unit, there are the

keys for starting/stopping the
pump, and displaying the
setting screens of the flow rate,
the maximum pressure limit,
and the minimum pressure limit.

<Starting/Stopping the Pump>

This is the key for starting/stopping the pump. Press to start

the pump, and press again to stop it. At the top of the key,
the LED is turned off when the pump is OFF (stopped) and turned
on when the pump is ON (running).

2-18
<Setting Pump Flow Rate>

F L OW R A T E S E T F L OW R A T E S E T - U P
0 9 . 9 0 m l ／m i n 0 9 . 9 0 m l ／m i n

( M A X : 1 0 . 0 0 m l ／m i n ) ( M A X : 1 0 . 0 0 m l ／m i n )

F L OW R A T E S E T - U P F L OW R A T E
0 9 . 9 0 m l ／m i n 09
F L OW 09 . 9 0 m l ／m i n ( M A X : 1 0 . 0 0 m l ／m i n ) ( MA X : 1 0 . 0
PRESS 0 . 0 MP a
UV 00 0 0
RI 00 0 0

Press the [FLOW SET] key to display the setting screen in the LCD
display. Use the arrow keys to set the numerical value at the
blinking cursor. After setting, press the [OK] key to update the
setting value.

2-19
 <Setting Pump Pressure Limits>

M A X I M UM S E T M I N I M UM S E T
0 2 . 0 MP a 0 0 . 4 MP a

( M I NS E T V A L ： 0 0 . 0 MP a ) ( MA X S E T V A L ： 0 2 . 0 MP a )

The current pressure is displayed in the normal screen of the LCD display.

Press the [MAXIMUM SET] key to set the maximum pressure limit.

Press the [MINIIMUM SET] key to set the minimum pressure limit.
M I M I M A M S E T
<Setting Maximum Pressure Limit> 0 0 . 1 M
( M A and
When it's over pressed, this function is to protect columns X : detectors.
5 0 . 0 0 M P a )
Press the [MAXIMUM SET] key to display the setting screen in the LCD
display. Use the arrow keys to set the numerical value at the blinking
cursor. After setting, press the [OK] key to update the setting value. When
the maximum pressure limiter is activated, the whole system stops and an M I M I M
error message appears.
( M
*"(MINSETVAL: 00.0 MPa)" displayed in the setting screen indicates the setting value of the
minimum pressure limit
<Setting Minimum Pressure Limit>
When it's pressed too low, this is to protect columns and solvent from
splitting. Press the [MINIMUM SET] key to display the setting screen in
the LCD display. Use the arrow keys to set the numerical value at the
blinking cursor. After setting, press the [OK] key to update the setting
value. When the minimum pressure limiter is activated, the whole system
stops and an error message appears.
*"(MAXSETVAL: 00.0 MPa)" displayed in the setting screen indicates the setting value of the
maximum pressure limit.
*For details on how to operate the arrow keys, refer to P.2-17.
*In case that the pressure limiter is activated to stop the system, refer to "Volume 4
Maintenance Manual" for trouble shooting.
*The maximum value cannot be less than the minimum value, and they cannot exceed
50.1 MPa.

2-20
 Detector Unit

UV DETECTOR RI DETECTOR

・In the [UV DETECTOR] and [RI DETECTOR] units, there are the keys for
adjusting the current UV/RI output to O mV and setting the recorder
signal range.

<Output Value Display Screen>

F L OW 09 . 9 0 m l ／m i n F L OW 09 . 9 0 m l ／m i n
PRESS 1 . 0 MP a PRESS 0 . 0 MP a
UV 02 1 0 UV 000
UV output 0
RI 00 3 5 RI 000
RI output 0

・In the LCD display, the current UV/RI output is displayed in mV unit.

UV Unit

・In the UV unit, there are the [AUTO ZERO] key for adjusting the UV
output to 0 mV, the [RANGE] key for setting the recorder signal range,
and the [SET] key for setting the detector response speed.

2-21
<Zero Adjustment Key>

・Press the [AUTO ZERO] key to adjust the current UV output to 0mV.
However, when the UV transmittance is too low, the AUTO ZERO
function may not work.

<Recorder Signal Range Adjustment>

UV RANGE SET
x001
↑ : RANGE UP
↓ : RANGE D OW N

・This function is used for output signal attenuation of pen recorder. Press
the [RANGE] key to display the setting screen in the LCD display.
Press the arrows key to increment the signal range (the peak will
be shown smaller), or to decrement it (the peak will be shown
larger).

*In case of monitoring the chromatogram with integrator, recorder signal range
buttons are without the need for setup.
*In case a peak-sense (sense threshold and peak height) fraction collector
(optional product) is used, the range need to be adjusted.

2-22
<Brightness Check>

UV S E T
*ME NU
P A R AM E T E R
BACK

UV S E T ( M E N U )
* S AMP L E 1000
R E F E R E NC E 1500
BACK
・This function is used to perform the brightness check of the lamp
installed on the UV detector. Press the [SET] key and select [MENU] to
display the output values of [SAMPLE] and [REFERENCE].

*If the [REFERENCE] value is too low, the lamp should be replaced.

D O Y O U S E T
<Setting Detector Response Speed>

Y E S

UV S E T
ME NU
* P A R AM E T E R
BACK

U V S E T ( P A R AM E T E R )
* R E S PONS E MID
( S L OW←→M I D←→F A S T )
BACK

U V S E T
・This function is used to set the detector response speed. Press the
* M E N U
[SET] key and select [PARAMETER] to display the response setting
P A R A M E T E R
screen. You can select the response speed from the three levels,
[SLOW], [MEDIUM], and [FAST]. They mean the time constants of 1.5
sec, 0.5 sec, and 0.05 sec respectively. To change the setting, press
the or arrow key.

U V S E T
M E N U
2-23
* P A R A M E T E R
RI Unit

・In the RI unit, there are the [AUTO ZERO] key for adjusting the RI
output to 0 mV, the [RANGE] key for setting the recorder signal range,
the [PURGE] key for replacing the solvent in the reference cell, and the
keys for selecting the polarity and whether or not to control the
temperature.

<Zero Adjustment Key>

・Press the [AUTO ZERO] key to adjust the current RI output to 0mV.
When the optical balance is lost too much, the AUTO ZERO function
may not work.

*If the zero adjustment function does not work, refer to "Volume 4 Maintenance
Manual".

<Recorder Signal Range Adjustment>

・Adjust the recorder signal range in the same way as described in

"<Recorder Signal Range Adjustment>" of the UV unit. For details, refer
to the page 2-22.

2-24
<Starting PURGE>

・This key starts/stops replacing the solvent in the reference cell. Press
to change the flow channel to the reference cell for one minute. At
the top of the key, the LED is turned off when the fraction is flowing
through the sample cell, and turned on when it is flowing through the
reference cell.

*During the [CLEAN UP] operation, the solvent in the reference cell is also
replaced automatically. This PURGE function may not be used in general
operation.

<Starting TEMP.C>

・This function is used to select whether or not to use the temperature

adjustment function of the RI detector. When the LED is turned on, the
temperature controlling heater is turned on to maintain the inside
temperature as 35℃ for stable operation. Normally this function should
be turned on. When you use a low boiling solvent, you can press the
[TEMP C] key to turn it off.

<Selecting SIGNAL POLARITY>

・There are cases that current RI output is shown in negative, when the
solvent with high index of refraction is used for mobile phase. This key
can be used to reverse the negative signal to positive. When the LED is
turned on, the signal is output as it is. Press the [POLARITY] key to turn
the LED off and reverse the polarity from negative to positive.

2-25
FRACTION Unit

・In the [FRACTION] unit, there are

the keys for selecting the method
of collecting fractions (siphon or
fraction nozzle) and setting the
dead volume.

<Setting DEAD VOLUME>

D E A D V O L UM E SET
* UV 0 1 . 9m l

( UV ←
→RI ←
→ ELSE )

・You can use this key to select the detector that detects the peak for
the basis of collecting fractions. Press the or arrow key
to select a detector. The time difference is calculated as dead
volume from the selected detector to a fraction nozzle. If you select
[ELSE], you can set an arbitrary value when pipe lines are extended
or other detectors are installed. To set an arbitrary value, press
and select [ELSE] to display the setting screen. Use the arrow keys
to set the numerical value at the blinking cursor.

*For details on how to operate the arrow keys, refer to P.2-17.

*The default value is preset in case our optional products (UV-254 NEXT,
UV-370 NEXT, or UV-600 NEXT for UV detector, and RI-700 NEXT for RI
detector) are installed.
*[ELSE] can be used when other detectors or non-standard-equipped fraction
collectors are installed.

2-26
<Siphon/Fraction Nozzle Selection Key>

・You can use this key to collect the fractions of eluent from the detector
by using the siphon or the fraction nozzle. The LED is turned off for the
eluent from the siphon, and turned on for the eluent from the fraction
nozzle. To collect fractions with the fraction nozzle, press when
the LED is off. Then the LED blinks, which indicates that the fraction is
passing through the dead volume specified by [DEAD VOLUME SET].
After passing through the dead volume, the eluent will flow from the
fraction nozzle. If you press again, the LED blinks in the same
way, and the eluent passes through the dead volume and then flows
from the siphon.

RECYCLE Unit

・This is the control unit of the

recycle valve. You can use the key
to start/stop the [RECYCLE]
operation.

<Starting RECYCLE>

・Press to start the [RECYCLE] operation, and press again

to stop the operation. At the top of the key, the LED is turned off
when the [RECYCLE] operation is stopped (normal flow channel), and
turned on when the [RECYCLE] operation is running.

2-27
CLEAN UP
Press the [CLEAN UP] key at the lower right of the operation panel to
execute the flow line cleaning automatically.

LCD display during CLEAN UP operation

S AMP L E L OO P C L E A N I NG CLEAN UP operation status

50%
S TART E ND CLEAN UP progress

In the LCD display, there are the two sections; one indicates the place
where the [CLEAN UP] operation is being executed, and the other the
operation progress.

<STATUS Display>
The upper section of the LCD display shows the place where the
[CLEAN UP] operation is being executed. For information on what the
display indication means, refer to the following table:

Display indication Where to clean

1.PUMP PURGE Solvent filter to Pump head
2. BUFFER CLEANING Buffer volume
3.SAMPLE LOOP CLEANING Manual injector sample loop
4.VALVE CLEANING Recycling valve
5.RI PURGE RI reference cell
6.FINISH CLEANUP PROGRAM

2-28
<CLEAN UP Progress>

S AMP L E L OO P C L E A N I NG

50%
S TART E ND

The lower section of the LCD display shows the progress of the
[CLEAN UP] operation. 100% means the completion of the operation.

<Emergency Stop>

If you press the [CLEAN UP] key during the [CLEAN UP] operation,
the LED is turned off, and the operation is stopped forcibly. Please
press the key only in emergency situation. After the emergency stop,
the display returns to the normal display.

<How to Operate>

When you press the [CLEAN UP] key, the operation procedures are
displayed in the LCD display. Please follow the instruction. When a
manual operation is required or when you need to verify settings, a
message appears in the LCD display with an intermittent beep. The
[CLEAN UP] operation ends in 10 to 15 minutes. After the completion,
turn the column selector valve back to its original position
([COLUMN]) to use it.

2-29
 <How to Use Solvent Suction Attachment>

In the LC-92XX NEXT, you must inhale the solvent by using a solvent
suction attachment (standard accessory) at the preparation for the
[CLEAN UP] operation.

Solvent Suction Attachment

<How to Use>

CLEAN UP preparation screen

1. When you press the [CLEAN UP]
5 P L E A S E P R I M I NG MUS T
B E DON E . key, the operation procedures 1 - 5
PUSH "OK " KEY ( 5 / 5 ) are displayed in the LCD display.
When you check and implement the
Siphon Box items 1 to 4, then the item 5
instructs you to inhale the solvent.

2. Set the solvent suction attachment

to the pump drain out inside of the
siphon box.

Pump Drain 3. Set the luer-lock syringe to the

Out solvent suction attachment.

Metal Part 4. Pull the syringe to inhale the solvent

(at least 10 mL of solvent must be
inhaled).

5. After inhaling the solvent, remove

the solvent suction attachment (pull
Solvent Suction Attachment out it while pushing the metal part
＋
located at the top of the pump drain
Luer-lock Syringe
out), and then press the [OK] key to
start the [CLEAN UP] operation.

2-30
 2-3

Chapter 3 Recycling Separation

Description of recycling separation, setting of recycling condition
parameter

2-31
Recycling Separation

The recycling system is a standard specification of the LC-92XX NEXT

series.
The recycling system is the feature that has the column eluent flow into
column inlet again.
If the separation is insufficient in normal HPLC separation, the change is
required in column (separation mode, length, particle size of packing)
and/or solvent condition (solvent polarity, gradient curve, salt
concentration).
Recycling system achieves hi-separation without change of such column
condition or solvent condition as the HPLC case.
Insufficient separation area is introduced into the same column once
more, and sufficient separation achieves.

Relation between Peak Separation and the number of

Recycling

In stead of using multiple columns, equivalent separation executes with

the Recycling separation. As shown below, separation is multiplied
according the numbers of the recycling.

Recycling separation:
COLUMN: JAIGEL-1H -F+JAIGEL-2H-F
FROW RATE: 3.5 ml/min
SAMPLE: Polystyrene oligomer (Mw. 495, Mw/Mn 1.20)

2-32
Separation efficiency with n times Recycling is shown following formula.

2(n+1) (TR2-TR1)
Rn+1=
{(n+1) W 12+nW E2}1/2+ {(n+1) W 22+nW E2}1/2

Rn+1 : Separation efficiency with n times Recycling

W1 : Width of Peak 1
WE : Peak diffusion width of flow line without column *
W2 : Width of Peak 2
TR1 : Retention time of Peak 1
TR2 : Retention time of Peak 2

T1 T2

Chromatograph

Peak shapes of Peak 1 and Peak 2

W2
W1

* When a column more than 20φ i.d. is used, W E does not affect the separation because it is negligible

smaller than the column volume. When Recycling executes many times, turn the MANUAL INJECTOR

knob to [LOAD] position for suppression of peak diffusion.

To take a separation such as the figure, Rn+11.5 is need.

2 2 1/2
*Peak width "W" with n times of recycling spreads to W(n+1)= {(n+1) W +nW E } .

2-33
Note of good Recycling Separation
Recycling separation may have the following troubles. Before the real
Recycling, pretest is recommended for the chromatogram acquisition
without Recycling.

When the Recycling area is too wide or the Recycle times are too much,
re-mixture trouble may occur. The top of recycled area will mix with the
tail of the recycled area. Recycling area and times should be suitable
value.

1st Cycle

2nd Cycle

3rd Cycle

5th Cycle

4th Cycle
6th Cycle

Re-mixture

Re-mixture

2-34
 <Overlap Trouble>
If the Recycling area is set too fast retention time, 2nd cycle peak get
past the 1st peak (symptom 1) or 2nd cycle peak overlaps with the later
peak (symptom 2). Pretest is recommended for the chromatogram
acquisition without Recycling to avoid overlap troubles.

Symptom 1

Symptom 2

A 1st Cycle peaks

without A

Recycle

chromatograph

Only A peak group

Recycle chromatograph

Normal chromatograph

2-35
 <Contamination Trouble>
The LC-92XX NEXT series standard specification has the backflow
prevention system. The backflow prevention system works to reduce
contamination during the recycle operation for good recycle separation.
The backflow prevention system has a buffer-volume. The buffer-volume
is cleaned during CLEAN UP operation. When the CLEAN UP is
neglected or high-viscosity sample/low soluble sample is recycled,
shock peak may appear at change over of the Recycling valve. When
the shock peak appears, do CLEAN UP operation again. If the symptom
cannot be remedied, cleaning-maintenance is required. Please refer to
Volume 4 "Maintenance Manual".

Recycle Chromatogram with contaminations.

0 20 40 60 80 100 120 Rt (min)

Recycle Chromatogram after CLEAN-UP

0 20 40
Rt (min)

2-36
 For good Recycling efficiency
During Recycling separation, recycled sample flows unique flow line that
the normal HPLC is not used, rear flow of the Detector, the Pump, and
the Injector flow line.
Select suitable instrument and turn over the knob of the MANUAL
INJECTOR to LOAD.

<Select Suitable Instrument>

In the LC-92XX NEXT series, the LC-9210 NEXT and LC-9225 NEXT
are selected according to the Column size.
Double plunger reciprocal style pump is applied to the LC-92XX NEXT
series. For the reduction of the diffusion, select suitable LC according
Column size.
Sample diffusion in the plunger room can be ignored when the Column
inside volume is enough larger than the plunger volume.

To Column

Plunger

It moves frontward and backward and solvent is

pressurized.

Plunger Volume The volume differs depending on the specification.

Structure of the Plunger

Table of Suitable Column Size φ for Recycling Separation

LC
Separation mode LC-9210 NEXT LC-9225 NEXT
SEC (Size exclusion
10φ  column id  30φ 10φ column id  50φ
chromatograph)
Partition/Adsorption/Other mode 10φ column id  20φ 10φ column id  30φ

LC
Type LC-9210 NEXT LC-9225 NEXT
Plunger volume 32 μL x 2 77μL x 2

2-37
 <Turn Over the Knob of the MANUAL INJECTOR to LOAD>
The LC-9210 NEXT has a 3 mL-Sample loop, and the LC-9225 NEXT
has a 10 mL-Sample loop as a standard.
When multiple times Recycling is done, turn over the knob to LOAD to
eliminate the sample loop from the flow line because the recycled
sample diffuse in the sample loop.

Wait the manual injector knob at the [INJECT] position while injecting the
sample, cleaning the needle port, and filling the sample loop with the
new solvent. After that, turn the manual injector knob to the [LOAD]
position.
When the pump flow rate of the LC-9210 NEXT is set at 3.0 mL/min or
more (10 mL/min or more for the pump flow rate of the LC-9130 NEXT),
wait for one minute after the injection, and then turn the manual injector
knob to the [LOAD] position to eliminate the sample loop from the flow
line.

Sample Loop Sample Loop

In the Flow Line Out of the Flow

Line
From Pump From Pump

To Column To Column

INJECT LOAD

2-38
 2-4

Chapter 4 Condition Setting

How to set conditions of LC-92XX NEXT series

2-39
Setting Operation

Pump setting

Set the pump flow rate and the pump pressure limit in the [PUMP] unit.

<Setting Pump Flow Rate>

・Press the [FLOW SET] key.
・The parameter to be changed blinks on the LCD display.
・Set the flow rate for the column.

<Setting Pump Pressure Limits>

・Press the [MAXIMUM SET] key].
・The parameter to be changed blinks on the LCD display.
・Set the value at which the pressure maximum limiter works.

The pressure maximum limiter stops the pump when the pump pressure rises over the
limit, so as to prevent a column and detector cell damage from the over pressure coming
from injection of dust and precipitated insoluble in a column. An error message appears
when the limiter works.

・Press the [MINIMUM SET] key.

・The parameter to be changed blinks on the LCD display.
・Set the value at which the pressure minimum limiter works.

The pressure minimum limiter stops the pump when the pump pressure drops below the
limit, so as to prevent a column damage or solvent spill when the solvent loses or leakage
of system occurs. An error message appears when the limiter works.

2-40
 <Limit of Setting Value>
The setting values of flow rate and pressure limits is limited in accordance
with the LC model and the set flow rate.
Refer to the following table. When the setting value is over the limitation, an
error message appears.

Flow Rate LC-9210 NEXT Flow Rate LC-9225 NEXT

 10.0 mL/min  25.0 MPa  9.9 mL/min  20.0 MPa
 19.9 mL/min  15.0 MPa
 25.0 mL/min  10.0 MPa

The flow rate can be set in the ranges of 0.1 to 10.0 mL/min and 0.1 to 30.0
mL/min for LC-9210 NEXT and LC-9225 NEXT, respectively.
To stop a pressure limiter, set [0 MPa].

Caution
When the Pump is running, the flow rate cannot be changed. Stop the Pump and reset the
flow rate. When the pump flow rate is changed, change the [MAXIMUM] pressure limit to
"monitoring pressure + 2.0 MPa" or more, and the [MINIMUM] pressure limit to "monitoring
pressure - 2.0 MPa" or less.

2-41
Setting UV detector
In the [UV] unit, the UV-370 NEXT, UV-254 NEXT, or UV-600 NEXT
function can be set.

<Recorder Signal Range Adjustment>

・This function is used for output signal attenuation of pen recorder. Press
the [RANGE] key to display the setting screen in the LCD display. Press
the arrows key to increment the signal range (the peak will be shown
smaller), or to decrement it (the peak will be shown larger).

*In case of monitoring the chromatogram with integrator, recorder signal range
buttons are without the need for setup.
*In case a peak-sense (sense threshold and peak height) fraction collector
(optional product) is used, the range need to be adjusted.

2-42
<Wavelength Selection>
・Pull the front door forward. UV Detector

Front Door

Pull forward

Wavelength

Selector
Locking Pin

Close

Releas

・Remove the locking pin of wave-length selector inside the front door.
・Turn the wave-length selector to set the wave-length.
・Tighten the locking pin.
・Close to the front door.
・Press the [AUTO ZERO] key in [UV DETECTOR].

*Setting method of UV-370 NEXT is above

mentioned. Model UV-254 NEXT detector uses only
one wave-length and not selected wave length.
*Select the wave-length at where using solvent has
low wavelength absorption. 0 adjustment may not do
at the wave-length where the solvent has high
absorption. Please refer P.2-51, for the typical
solvent UV cut-off wavelength.
*Setting cut filter (Only UV-600 NEXT)
Wave-length Cut filter
199 nm ～ 399 nm Push
400 nm ～ 600 nm Pull

2-43
Setting RI detector
In the [RI] unit, the RI-700 NEXT function can be set.

<Recorder Signal Range Adjustment>

・This function is used for output signal attenuation of pen recorder. Press
the [RANGE] key to display the setting screen in the LCD display.
Press the arrows key to increment the signal range (the peak will
be shown smaller), or to decrement it (the peak will be shown
larger).

*In case of monitoring the chromatogram with integrator, recorder signal range
buttons are without the need for setup.
*In case a peak-sense (sense threshold and peak height) fraction collector
(optional product) is used, the range need to be adjusted.

<Setting SIGNAL POLARITY>

・When the LED at the top of [POLARITY] is turned on, the output has the
same polarity as the original signal. Press the [POLARITY] key to turn
the LED off and reverse the polarity of the output signal.

*Reverse polarity is profitable when high refractive index solvent like toluene is used for
eluent.
*Please refer P.2-51, for the typical solvent refractive index.

2-44
<Setting TEMP C>

・When the LED is turned on, the temperature controlling heater of the
detector is on to maintain the temperature at 35℃. Press the [TEMP C]
key to turn the LED off, which indicates that the temperature is not
controlled.

*When the LED is turned on, more stable RI signal is obtained. Normally, the LED should
be turned on.
*Using low boiling point solvent, turn the LED off to suppress bubbling of the solvent.
*Please refer P.2-51, for the typical solvent boiling point.

2-45
Setting fraction
In the [FRACTION] unit, the dead volume can be set.

<Setting DEAD VOLUME>

・Press the [DEAD VOL SET] key.
・Selecting [UV], set the preset dead volume between UV-370 NEXT,
UV-254 NEXT, or UV-600 NEXT, RI-700 NEXT, and Nozzle of Fraction
Collector.
・Selecting [RI], set the preset dead volume between RI-700 and Nozzle of
Fraction Collector.
・When the external detector is used, or when the fraction collector is
removed, select [ELSE] to set the dead volume.
・The specified dead volume is used to automatically calculate the dead
volume flow time when the valve to be used is switched between the
fraction nozzle and the siphon.

*The default value is preset in case our optional products (UV-254 NEXT, UV-370
NEXT, or UV-600 NEXT for UV detector, and RI-700 NEXT for RI detector) are
installed.
*If [0.0] value is selected, the dead volume (delay time) is neglected. Immediately after
pressing the [Co/Dr] key, the valve to be used is switched between the fraction nozzle
and the siphon.
*When the [RECYCLE] operation is switched to ON/OFF, the dead volume (delay time) is
neglected. Immediately after pressing the [RECYCLE] key, the valve is switched.

2-46
 2-5

Chapter 5 Appendix
Degassing, property of typical organic solvent, elimination of micro
particles

2-47
Degassing
Solvent to use an eluent in the LC-92XX should be degassed. Pressure
fluctuation of the peak retention time or the detector noise may occur with
the babble in undegassed solvent. To result best chromatograph without
noise and fluctuation, the solvent to use an eluent should be degassed.

<Degassing with ON-LINE DEGASSER>

・The ON-LINE DEGASSER should be connected between the solvent
container and LC-92XX NEXT at the solvent supplying connector.
・Read the ON-LINE DEGASSER instruction and according to the
instruction, connect the ON-LINE DEGASSER to the LC-92XX NEXT.
・According to the instruction, turn the power switch on to make sure that the
AC voltage is suited to the DEGASSER.

Be care that the DEGASSER is chemical proof against the solvent

according to the instruction.
Strong organic solvent is sometime highly corrosive to the DEGASSER.
In case the solvent which is corrosive to the DEGASSER, do degassing
with an Ultra sonic cleaner and or vacuum pump described below.

In water solvent operation, the degassing may not be sufficient by the

ON-LINE DEGASSER due to the flow rate and laboratory temperature
environment.
Degassing with an Ultra sonic cleaner and or vacuum pump is also
recommended.

The above method is a typical connecting method.

The connecting method may differ depending on the type of degasser or
pipe line handling because these factors may cause a different filtering
resistance.
Make sure to apply the suitable connection without much flow resistance
according to the instruction.

2-48
<Degassing with Ultra Sonic Cleaner>
・Move the solvent into the container. In case of
mixture solvent, stir the solvent thoroughly.
Without stirring the mixture solvent, the
reproducibility of the chromatogram may be lost,
because the lost of the component may occurs.

・Solvent such as sealed containers without lids,

and a lid on things such as Kleenex for good
ventilation.
If the seal is inflated when the mixing
temperature ultrasonic, solvent containers may
be destroyed.

・Dip the container in the Ultra sonic cleaner. Ultra sonic cleaner should be
half full with water.

・Operate an ultra sonic cleaner in 10 to 15 min. When there is no bubbling,

degassing is complete.
When bubbles stuck on the wall of solvent container, blow N2 or He2 gas
in the solvent easy to remove the bubbles. After blowing, operate an ultra
sonic cleaner in few minutes.
In case of the solvent mixture, keep in short time operation of the ultra
sonic cleaner less than 30 min.
Vaporization of the solvent to change composition of the mixture occurs in
long operation of the ultra sonic cleaner.

・Wipe the drops out of the container and place the container on the solvent
stage in the LC-92XX NEXT.

Dissolvent of the air into the solvent occurs soon after degassing. Do
degassing just before use the solvent.

2-49
<Degassing with Ultra Sonic Cleaner Plus Vacuum>
To aspirator
・Move the solvent into the container. In case of
mixture solvent, stir the solvent thoroughly.
Without stirring the mixture solvent, the
reproducibility of the chromatogram may be lost,
because the lost of the component may occurs.
The solvent container must be vacuum tight. A
conical beaker or conical flask may be
damaged at decompression.

・Vapor from the solvent contains organic vapor. To prevent spill of the
organic vapor to swage, use closed system aspirator to make vacuum.
Waste water should be disposed properly according to laws and
regulations in the same manner as organic solvents.

・Place the container in the ultra sonic cleaner, and seal it with a rubber
stopper that is fitted into the container mouth. Set a vacuum line to the
rubber stopper, and connect its one end to the aspirator.

・Perform the ultra sonic cleaning, and operate the aspirator to reduce the
pressure of the container. Operate both the ultra sonic cleaner and a water
aspirator in 5 min.

Solvent component will be change while the vacuum-filtration. Keep the

condition of the filtration

time and vacuum condition. Keep the vacuum degree and time in
constant.

・Wipe the drops out of the container and place the container on the solvent
stage in the LC-92XX NEXT.

Dissolvent of the air into the solvent occurs soon after degassing. Do
degassing just before use the solvent.

2-50
 Property of typical organic solvent

<Solvent Property List>

Viscosity UV limit Refractive Boiling Viscosity UV limit Refractive Boiling
Solvent ε(Al2O3) (mPa s; to use index point Solvent ε(Al2O3) (mPa s; to use index point

20℃) (nm) (20℃) (℃) 20℃) (nm) (20℃) (℃)

Pentane 0.00 0.24 200 1.357 36.1 Acetone 0.56 0.30 330 1.359 56.3

Hexane 0.01 0.31 200 1.375 68.7 Methyl ethyl ketone 0.51 0.42 329 1.379 79.6

Heptane 0.01 0.42 200 1.388 98.4 iso-Butyl methyl ketone - 0.54 334 1.396 116.5

2,2,4-triMethylpentane 0.01 0.50 215 1.391 99.2 Diethyl ether 0.38 0.24 218 1.352 34.6

Nonane - 0.72 200 1.405 150.8 Diisopropyl ether 0.28 0.37 220 1.369 69.0

decane 0.04 0.92 210 1.412 174.1 Tetrahydrofuran 0.45 0.55 212 1.407 66.0

Cycropentane 0.05 0.47 210 1.409 49.3 1,4-Dioxane 0.56 1.44 215 1.442 101.3

Cycrohexeane 0.04 0.98 200 1.426 80.7 Propylene carbonate - - - 1.419 240.0

Benzene 0.32 0.65 278 1.501 80.1 Methanol 0.95 0.55 205 1.328 64.7

Toluene 0.29 0.59 284 1.497 110.6 Ethanol 0.88 1.20 210 1.361 78.3

o-Xylene 0.26 0.81 288 1.505 144.4 1-Propanol 0.82 2.26 210 1.386 97.2

m- Xylene 0.26 - 290 1.496 138.5 2-Propanol 0.82 2.86 205 1.377 82.3

Dichloromethane 0.42 0.45 233 1.424 39.8 2-Methoxyethanol - 1.72 210 1.402 124.6

Chloroform 0.40 0.58 245 1.446 61.2 1-Butanol - 2.95 215 1.399 117.7

Carbon tetrachloride 0.18 0.97 263 1.460 76.8 2-Butanol - 4.21 260 1.397 99.6

1,2-Dichloroethane 0.49 0.79 230 1.445 83.4 Isobutanol - 4.70 200 1.396 107.7

Trichloroethylene - 0.57 273 1.477 87.2 2- Ethoxyethanol - 2.05 210 1.408 135.6

Tetrachloroethene - 0.93 295 1.506 121.2 Acetonitrile 0.65 0.34 190 1.344 81.6

n-Butyl chloride - 0.47 220 1.402 78.4 Diethylamine 0.63 0.38 275 1.387 56.0

Chlorobenzene 0.30 0.80 287 1.525 131.7 N,N-Dimethylformamide - 0.92 268 1.430 153.0

o-Dichlorobenzene - 1.32 295 1.551 180.5 N,N-Dimethylacetamide - 2.14 268 1.438 166.1

Disulfide 0.15 0.37 380 1.626 46.3 Pyridine 0.71 0.95 330 1.510 115.3

N- Methyl
Methyl acetate 0.60 0.37 260 1.362 56.3 - 1.67 285 1.488 202.0
-2-Pyrrolidone

Ethyl acetate 0.58 0.46 256 1.372 77.1 Dimethylsulfoxide 0.62 2.20 286 1.478 189.0

Butyl acetate - 0.73 254 1.394 126.1 acetic acid 大 1.31 - 1.372 117.9

Methoxyethyl acetate - - 254 1.402 144.5 Water 大 1.00 - 1.333 100.0

2-51
<Miscibility of Solvent>
Acetic acid

Acetone

Acetonitrile Immiscibility

Benzene

Butanol Miscibility

Carbon tetrachloride

Chloroform

Cycrohexeane

Cycropentane

Dichloroethane

Dichloromethane

Dimethylformamide

Dimethylsulfoxide

Dioxane

Methyl acetate

Ethanol

Diethyl ether

Heptane

Hexane

Methanol

Methyl ethyl ketone

Octane

Pentane

Propanol

Dipropyl ether

Tetrachloroethane

Tetrahydrofuran

Toluene

Trichloroethane

Water

Xylene
Acetic acid

Acetone

Acetonitrile

Benzene

Butanol

Carbon tetrachloride

Chloroform

Cycrohexeane

Cycropentane

Dichloroethane

Dichloromethane

Dimethylformamide

Dimethylsulfoxide

Dioxane

Methyl acetate

Ethanol

Diethyl ether

Heptane

Hexane

Methanol

Methyl ethyl ketone

Octane

Pentane

Propanol

Dipropyl ether

Tetrachloroethane

Tetrahydrofuran

Toluene

Trichloroethane

Water

Xylene

Replace solvent for a column should be easy soluble chemical against the
solvent pre-used. Make sure that a solubility is good each other. When
changing solvents are poor solubility each other, select the intermediate
solvent which has good solubility to each other.

In case salt contained solvent, the intermediate solvent which has a good
solubility to salt, then replace the final solvent.

2-52
Micro particle elimination
To come in Micro particles such as dust and insoluble, the LC-09XX and the
column may be damaged. Such micro particles should be eliminated before
the application.

<Elimination of Micro Particles in Solvent>

・With Membrane filter of 0.45μm,
filtrate the solvent using aspirator.
Solvent
・Vapor from the filtering flask
contain organic solvent. To
Funnel
prevent spill of the organic vapor to
To aspirator
Membrane filter swage, use closed system
aspirator to make vacuum.
Waste water should be disposed
Filtering flask properly according to laws and
Filtered solvent
regulations in the same manner as
organic solvents.

In case clear appearance, some time the solvent contain micro particles,
so make sure to eliminate the micro particles.

Solvent component will be change while the vacuum-filtration. Keep the

condition of the filtration time and vacuum condition. Keep the vacuum
degree and time in constant.

2-53
<Micro Particle Elimination in Sample Solution>
・With Membrane filter of 0.45μm, filtrate the
sample solution using pressure of a
syringe.

Syringe ・If the sample solution is more than 100ml

do the same elimination of the solvent
following the instructions of the elimination
of micro particles in solvent above
mentioned.

Disposable Membrane Filter

Filtered Sample
The sample may contain the micro particles. Make sure to eliminate the
micro particles.

Needle port cleaning

The overflowed sample solution may contaminate the needle port of the
injector when injecting the sample with the manual injector.

After injecting the sample with the manual injector, be sure to rinse and
clean the needle port described as follows.

・Remove the Syringe Needle from the syringe

and attach Needle Port Guide.

・Suck pure solvent in the syringe and clamp the

needle port guide on the needle port of the
manual injector.
・Set the manual injector knob at the [INJECT]
position to perform needle port cleaning.

・Repeat 3 times this cleaning.

2-54
 Index
(Each number indicates a page number within "Volume 2 General Information Manual". Please refer to

the actual page number "2-*".)

Micro Particle Elimination in Sample

［ Ｅ Solution · 54

MINSETVAL · 20
[MAXIMUM SET] Key · 20, 40 Elimination of Micro Particles in Solvent ·
Miscibility of Solvent · 52
[MINIIMUM SET] Key · 20 53, 54

[OK] Key · 17, 19, 20, 30 ELSE · 26, 46

Ｎ

Ａ Ｆ Needle port cleaning · 54

Non-diffusion Function · 8, 10, 36

Arrow Keys · 17, 19, 20, 22, 23, 26, 42, 44 Flow Channel Map · 11
Note of good Recycling Separation · 34
For good Recycling efficiency · 37
Null Glass Adj. Hole · 5

Ｂ Fraction Nozzle · 3, 4, 7, 12, 26, 27, 46

FRACTION Unit · 26
Brightness Check · 23
Ｏ
FROM COLUMN Connector · 5

Operation Panel · 1, 3, 8, 9, 14, 15, 28

Ｃ Ｌ Output Port · 6

Overlap Trouble · 35
CLEAN UP · 17, 25, 28, 29, 30
LCD Display · 16, 19, 20, 21, 22, 28, 29, 30,
Column Holder · 5
40, 42, 44
Column Reject Valve · 3
Ｐ
Limit of Setting Value · 41
Contamination Trouble · 36
Locking Pin · 43 Power Switch · 4, 6
Cut Filter Exchanger · 5
Luer-lock Syringe · 30 Pre-Column Holder · 5

Pump Drain Out · 7, 30

Ｄ Ｍ Pump Drain Valve · 3, 7

Pump Head · 4, 28
Degassing with ON-LINE DEGASSER ·
Manual Injector · 5
PUMP Unit · 18
48
Manual Injector Valve · 3
Degassing with Ultra Sonic Cleaner · 49,
Manual Sample Injector · 7, 8, 9, 28, 38, 54
50
Ｒ
Maximum Discharge Pressure · 8

MAXSETVAL · 20 Recorder Signal Range Adjustment · 22,

24, 42, 44

2-55
RECYCLE Unit · 27 Siphon · 3, 4, 7, 12, 26, 27, 30, 46 Ｕ
Recycling Separation · 32 Siphon Box · 3, 4, 7, 30
UV Detector · 4, 23, 26, 46
Relation between Peak Separation and Solvent Property List · 51
UV DETECTOR · 21, 43
the number of Recycling · 32 Solvent Suction Attachment · 30
UV Unit · 21, 24
RI Detector · 4, 25 Solvent Table · 4

RI DETECTOR · 21 Specification · 8

RI Unit · 24 Starting PURGE · 25 Ｗ

Starting RECYCLE · 27
WAKE UP · 16
Ｓ Starting TEMP.C · 25
Wave Length Tuner · 4
Starting/Stopping the Pump · 18
Wavelength Selection · 43
Selecting SIGNAL POLARITY · 25
STATUS Display · 28
Setting DEAD VOLUME · 26

Setting Detector Response Speed · 21, Ｚ

Ｔ
23
Zero Adjustment Key · 22, 24
Setting Pump Flow Rate · 19 TO COLUMN Connector · 5

Setting Pump Pressure Limits · 20, 40, 41

2-56
 VOLUME

Volume 3 Installation Manual

Check list of accessories, inspection sheet and shipping data
Contents

Volume 3 Installation Manual

Chapter 1 Place to Setup .................................................. 3-1
Installation Location ................................................................ 3-2
Installation Conditions ............................................................. 3-3
Chapter 2 Pipe Lines/Wiring Connections ........................ 3-4
Mounting Knobs/Serial Number Check ................................... 3-5
Pipe Line Connections ............................................................ 3-6
Wiring Connections ............................................................... 3-10
Chapter 3 Validation after Installation ..............................3-13
Preparation for the validation ................................................ 3-14
Sample for Validation ............................................................ 3-15
Pipe Line and Start Cable Test ............................................. 3-16
Wiring Connections and Valve Validation ............................. 3-18
Preface

This manual is for JAI staff to install the LC-92XX NEXT series
properly.

Please be advised that installation and relocation by users are not

suggested.

The installation and relocation by users self are unwarrantable.

For more details of operation, information, and maintenance of the

instrument, please refer to other volumes, "Daily Operation Manual",
"General Information Manual", and "Maintenance Manual" on the
tabs.

There are checklist of accessories, shipping data and inspection

sheet attached at the end of this chapter.
 3-1

Chapter 1 Place to Setup

Preparation to set up LC-92XX NEXT series

3-1
Installation Location

Place the LC-92XX NEXT series at the location and environment that satisfy the
following conditions for correct operation.

Installation Location

The LC-92XX NEXT requires space at least more than 500 mm (W) x 600 mm (H) x
600 mm (D). Depth may need 750mm for an air vent and power supply parts at the
back of the body. For left and right of the body, may need extra 100mm each for
enough space.

The table to place the body has to be horizontally stable and capable to hold the
weight of the body.

Please prepare an enough space for optional accessories and attachments as well.

Environment

･Indoor temperature must be between at 4 to 35 degree (c) with no dew condensation.

･Must not have temperature changes during operation.

･Indoor humidity must be within 25 to 85%.

･Must be well ventilated.

･Must not produce corrosive gas nor be filled with it.

･Should not be exposed to direct sunlight and direct wind.

･Should be supplying the same working voltage without sudden change.

･Must not give tremor and or shock.

･Must not have strong magnetic field interference.

･Should avoid dusts around.

3-2
Installation Conditions

Place the LC-92XX NEXT series at the location that satisfies the following installation
conditions for correct operation.

Power condition

Power voltage: AC 100V to 240V (within 10% variation voltage)

Frequency: 50Hz or 60Hz

Power supply: Requires 500VA at minimum and 800VA at maximum.

For additional business machines, prepare enough power

supply.

Electrical sockets: It has a 3 pins plug with a ground connection (Type A). In case
of none ground connection socket or different type of plug,
please be advised to use an extension and/or adapter to make
it 2 pins plug and connect ground terminal.

3-3
 3-2

Chapter 2 Pipe Lines/Wiring Connections

Pipe lines/wiring connections of the LC-92XX NEXT series

3-4
Serial Number Check

<Serial Numbers>
･Check out the serial numbers of the body and detectors.

3-5
Pipe Line Connections

Follow the instructions to connect the pipes appropriately to use the LC-92XX NEXT
series.

<Inside of the Front Door>

<Install the detector(s).>
Front Door

1. UV Detector Tray

2. UV Screw

3. RI Detector Tray

Drain Valve 4. RI Screws

・ Pull the front door to open.

・ Take out the plastic bag from inside of the body and unscrew all the seal
plugs.

・ Draw out the UV detector tray.

・ Place an UV detector on the tray and retain the UV detector by the UV

screws.

・ Push back the UV Detector tray into the body thoroughly.

・ Draw out the RI detector tray.

・ Place a RI Detector on the tray. Try to avoid the drain valve.

・ Retain the RI Detector on the tray by the RI screws.

・ Push back the UV Detector tray into the body thoroughly; make sure the
other pipe lines.

3-6
 <Pipe Connection>
<A line: Pump out tee joint – Pump drain valve>

A
・ Connect the A pipe between the tee joint of the
pump out and the pump drain valve.
UVD
Pump

Pump Drain Valve

<B Line: Pump drain valve – FROM PUMP union>

UVD ・ Connect the B pipe between the FROM PUMP

union connector and the right side of the drain
valve.

B FROM PUMP union

Pump Drain Valve

<UV detector– TO UV union>

・ Connect a pipe between the UV detector cell (the

inlet port / lower part) and the TO UV union.
UVD

TO UV union

Pipe lines set up view

3-7
 <UV detector cell (up part) – UV-RI union (IN)>
<C line: UV-RI union – RI detector (IN)>

UVD

・ Connect a pipe line between the UV detector cell

(up part) and the UV-RI union.

・ Connect the C pipe between the UV-RI union

C (lower part) and the RI detector (IN).
RID

UV-RI Union

<D line: Valve union behind drain valve – RI (out) >

<E line: Manual injector drain port – Manual injector>
<F line: Pump drain valve – Pump drain port>

・ Connect the D pipe line between the upper part of

UVD the valve union behind the drain valve and the RI
(out).

・ Connect the E pipe line between the manual

Manual Injector
injector and the manual injector drain port of the
Drain Port
siphon box.
D
F
E

Siphon Box
・ Connect the F pipe from underneath the pump
RID drain valve to the pump drain port of the siphon
box.

Valve Union
Pump Drain Port (Behind the Pump Drain Valve)

Pipe lines set up view

3-8
 <Solvent supply port – solvent suction line (with line filter)>

<Total waste – a waste reservoir>

Solvent Solvent
Supply Port Filter ・ Connect the solvent suction line (with line filter) to
the Solvent supply port.

・ Connect a tube from underneath of the siphon box

into a waste reservoir.

Total Waste
Into a waste
reservoir

3-9
Wiring Connections

Follow the instructions to connect the wiring appropriately to use the LC-92XX NEXT
series.

<Main Body to AC Power>

・Put the power cable into [AC INLET 100 ～

240 V] at the back of the body.

Unavailable

To AC
AC INLET
power 100V

3-10
 <Main Body to Integrator (or Recorder)>
・Connect an integrator (or a recorder) signal
Output Port cable to the output port (16 terminals).

Port No. Description

1 UV +Output for an integrator
2 UV -Output for an integrator
3 RI +Output for an integrator
4 RI -Output for an integrator
5 UV +Output for a recorder
6 UV -Output for a recorder
Left hand of 7 RI +Output for a recorder
the body 8 RI -Output for a recorder
9 START +
10 START -
11 Recycle signal +
12 Recycle signal -
13 Collect valve +
14 Collect Valve -
16 |15|14|13|12|11|10 | 9 | 8 | 7 | 6 | 5 | 4 | 3 | 2 | 1 15 Siphon counter +
16 Siphon counter -

Top of Output Terminal Block

・Connect each signal cable to the

corresponding output port as shown in the
Wire Release above table, by referring the description below.
Hole
・To connect, use a flat head screwdriver and
put it into a wire release hole. Insert a cable
into a wire insert hole.
・Remove the screwdriver from the wire release
Wire Insert
Hole
hole and make sure that the wire end is
locked tight in the wire insert hole.

*Minus signals at recycle signal(12), collect valve(14), and siphon counter signal(16) are the same. In use of an
electronic device such as JDS series to read several signals at once, joint the outputs with a jumper wire and
connect as one output port.

3-11
 <Connect to JDS Series (Sold as optional accessory)>
<Connect to a JDS signal cable>
・Connect the cable between the D sub-
miniature connector and the JDS signal inputs
[H] and [L].
･In use of 2 different detectors, connect another
signal inputs [H] and [L] to output port + (3)
and – (4) as well.
(The left image shows only the UV detector.)

<Connect to JDS 25P D sub-miniature connector>

･Insert JDS 25P D sub-miniature connector to
Top of Output Terminal Block
the JDS.
･Connect the signal cables to the output ports
of the LC-92XX NEXT series according to the
16 |15|14|13|12|11|10 | 9 | 8 | 7 | 6 | 5 | 4 | 3 | 2 | 1 numbers printed on the mark tubes.

･Check all cable connections.

TO JDS

*Each signal cable is attached with a mark tube

on which the number is printed.

Check Cable Connection

Cable connection is completed □

3-12
 3-3

Chapter 3 Validation after Installation

LC-92XX NEXT series validation after the installation

3-13
Preparation for the validation

The items listed below are required to validate that the LC-92XX NEXT series is
installed correctly. * 1 items are standard equipped. * 2 items are prepared by JAI staff.
Those without *1 or *2 should be prepared by customer side.

Upon customer's request, we can execute validation in a different way, after mutual
agreement instead of the following standard validation.

・ Solvent: Chloroform or Methanol 500ml or more.

・ Waste container or bottle 3L capacity or bigger.
・ A beaker 100 ml sized or bigger.
・ Sample vial 2 pcs.
・ Toluene Small amount
・ An integrator or a recorder. (In case of purchasing our system with NEXT
seriese, JAI staff will set it up at once.)
・ *1 Hamilton syringe 1 pc.
・ *1 Luer lock syringe 1 pc.
・ *1 8 mm wrench spanner 1 pc.
・ *2 A dummy pressure sensor 1 pc.

3-14
Sample for Validation

Prepare the validation sample for the LC-92XX NEXT series.

・ Measure 1.3ml toluene with a hamilton syringe.

・ 0.3ml toluene out of 1.3ml to a sample vial with hamilton syringe.
・ Prepare chloroform or methanol in a beaker.
・ Take out chloroform or methanol from the beaker by hamilton syringe. 20ml of
chloroform or methanol to the sample vial and dilute the toluene.

3-15
Pipe Line and Start Cable Test

Check the pipe line connection and the start cable connection of the LC-92XX NEXT
series.

･Remove the column cover from the right side of the instrument, and connect a
dummy pressure sensor between [TO COLUMN] and [FROM COLUMN] using a
wrench.

･Dip the solvent filter into methanol or chloroform. Place the solvent container on
Solvent table located upper shelf of LC-92XX NEXT series.

･Refer to "Chapter 2 General Operation" in "Volume 1 Daily Operation Manual" to

turn the power on and execute the [CLEAN UP] operation.

･When [PUMP PURGE] is displayed on the screen during the [CLEAN UP]
operation, check the solvent out from the pump drain out in the repeat injector box.
In the LC-92XX NEXT, you must inhale the solvent by using a solvent suction
attachment at the preparation for the [CLEAN UP] operation.

・ Settings
Settings
Flow Rate 9.0 mL/min

[MAXIMUM] 0
Pressure Limiter
[MINIMUM] 0

Injector 3 ml (3 times injection)

254 nm
Detector UV*1
In case of pen recorder, RECORDER SIGNAL RANGE x 1
TEMP. CONTROL ON (LED is ON)
SIGNAL POLARITY POSITIVE (LED is ON)
Detector RI
In case of pen recorder ,RECORDER SIGNAL RANGE (Methanol
x 1, Chloroform x 4)

*1 This is the case of the optical pass length of 0.5 mm. With other optical pass length, adjust
the concentration of sample accordingly.

3-16
・ Start Pump.
・ Make sure that the pump pressure is stable.
Check Column Pressure Stability
Column pressure is stable. □

・ Refer to the page 1-8 at Volume 1 to perform the zero adjustment of the
detector.
・Set up the integrator or recorder.

・Set the acquisition time to 1.5 min with the integrator.

・ Refer to the page 1-10 at Volume 1 to inject the sample.
・Check no leakage from flow line inside of the front door, connectors of fraction
collector and connectors of dummy pressure sensor during the chromatogram
acquisition.

Leak Check
Inside of the front door □
Connectors of dummy pressure sensor □

･Repeat the operation three times.

･When using the integrator, check whether 3 chromatograms are obtained, and
whether the peaks are similar in visual. When using the recorder, check that three
peaks are obtained and that they are similar in visual.

Check Chromatogram
Three chromatograms or peaks are obtained □
Peaks are similar in visual □

･Press the [ON/OFF] key in [PUMP] to stop the pump.

3-17
Wiring Connections and Valve Validation

Check the wiring connections and valve operation of the LC-92XX NEXT series.

・ Refer to "Volume 1 Daily Operation Manual" to enter the following values:

Settings
Flow Rate 9.0 mL/min
[MAXIMUM] 0
Pressure Limiter
[MINIMUM] 0
Injector SAMPLE 1 ml
254 nm
Detector UV*1
In case of pen recorder, RECORDER SIGNAL RANGE x 4
TEMP. CONTROL ON
SIGNAL POLARITY POSITIVE
Detector RI
In case of pen recorder, RECORDER SIGNAL RANGE (Methanol
x 4, Chloroform x 8)

・ Turn on the pump.

・ Refer to the page 1-8 to perform the zero adjustment of the detector.
・ Set the acquisition time to 30 min with the integrator.
・ Refer to the page 1-10 at Volume 1 to inject the sample.
・ 10 seconds after the start, move the manual sample injector to the [LOAD]
position, and press in [RECYCLE] to monitor the chromatogram.
・ Make sure that peak is in recycling.
Check of Recycling Chromatogram
・ Peak is recycling □
[R] mark appears on the Chromatogram (JDS acquisition) □

3-18
･After checking the recycling peak, press in [RECYCLE] to stop the

[RECYCLE] operation. Then press in [FRACTION] and check that the eluent

is flowing from the fraction nozzle.

Check Collect Chromatogram

Check the collect valve □
[C1] mark appears on the Chromatogram (JDS acquisition) □

・ After checking the collect valve, press in [FRACTION] and check that the

eluent stops running from the fraction nozzle.

Check Collect Chromatogram

Check the collect valve □
[D1] mark appears on the Chromatogram (JDS acquisition) □

･Press the [ON/OFF] key in [PUMP] to stop the pump.

3-19
 VOLUME

Volume 4 Maintenance Manual

Maintenance and trouble shooting of LC-92XX NEXT series
(except for optional parts)
Contents

Volume 4 Maintenance Manual

Chapter 1 Maintenance of LC-92XX NEXT ........................... 4-1
Replacement of Column ............................................................... 4-2
<Installing Column> ............................................................................ 4-2
<Removing Column> .......................................................................... 4-5
Cleaning of Solvent Filter ............................................................. 4-6
<Cleaning Solvent filter> ..................................................................... 4-6
Maintenance of Manual Injector ................................................... 4-7
<Removing Manual Sample Injector> .................................................. 4-7
<Replacing Rotor Seal and Stator Face> ............................................ 4-8
<Installing Manual Sample Injector> .................................................... 4-9
Maintenance of Pump ................................................................ 4-10
<Replacing Pump Seal>.................................................................... 4-10
<Cleaning Check Valve, Replacing Check Valve Seal> ..................... 4-14
Pipe Connection ......................................................................... 4-18
<Pipe and Connector, Name and Shape> ......................................... 4-18
<Pipe Line Connections> .................................................................. 4-18
Adjustment of RI Detector .......................................................... 4-20
<Optical Alignment of RI Detector> ................................................... 4-20
Manual Cleaning Instruction of Buffer Volume ........................... 4-21
<Manual Cleaning of Buffer Volume> ................................................ 4-21
Chapter 2 Trouble Shooting ................................................ 4-22
Chromatogram Acquisition Error ................................................ 4-23
<Base Line Wander 1> ..................................................................... 4-23
<Base Line Wander 2> ..................................................................... 4-24
<Base Line Drift> .............................................................................. 4-26
<Base Line Drift during Recycling Operation> ................................... 4-28
<Poor Recycling Efficiency> .............................................................. 4-29
Abnormal Pressure .................................................................... 4-30
<Abnormal High Pressure> ............................................................... 4-30
<Abnormal Pressure Fluctuation> ..................................................... 4-31
<Abnormal Low Pressure> ................................................................ 4-33
Abnormal Signal ......................................................................... 4-35
<Fail Power>..................................................................................... 4-35
 <No Signal and No Event Mark> ....................................................... 4-35
<UV Detector Abnormal Signal>........................................................ 4-36
<RI Detector Abnormal Signal> ......................................................... 4-36
Error Message ........................................................................... 4-37
<ERROR 01>.................................................................................... 4-37
<ERROR 02>.................................................................................... 4-37
<ERROR 03>.................................................................................... 4-38
<ERROR 04>.................................................................................... 4-38
<ERROR 05>.................................................................................... 4-39
<ERROR 06>.................................................................................... 4-39
<ERROR 07>.................................................................................... 4-39
<ERROR 08>.................................................................................... 4-40
<ERROR 09>.................................................................................... 4-40
<ERROR 10>.................................................................................... 4-40
<ERROR 11>.................................................................................... 4-41
<ERROR 12>.................................................................................... 4-41
<ERROR 13>.................................................................................... 4-41
<ERROR 14>.................................................................................... 4-42
<ERROR 15>.................................................................................... 4-42
<ERROR 16>.................................................................................... 4-42
<ERROR 17>.................................................................................... 4-43
<ERROR 18>.................................................................................... 4-43
<ERROR 19>.................................................................................... 4-44
<ERROR 20>.................................................................................... 4-44
<ERROR 21>.................................................................................... 4-45
Preface

Fundamental maintenance and trouble shooting of LC-92XX NEXT

series are described in this manual.

Refer to the paragraph for your successfully operation. Prevent solvent

leak and electrical shock during the maintenance.

Normally cut off the AC power. Be careful of electrical shock during the
maintenance when power switch turn on.
 4-1

Chapter 1 Maintenance of LC-92XX NEXT

Description of LC-92XX NEXT series maintenance

4-1
Replacement of Column
If the separation is required with the different type column, replacement of column is
needed.
Surely stop the pump during the replacement of column to prevent solvent spill.

<Installing Column>
Install the column as following.

･Turn the column reject valve to the [REJECT] position.

･Remove the column cover of the LC-92XX NEXT from the chassis.

･Hold the column(s) on the column holder inside of the column cover.

Column

TO COLUMN Connector

Column Reject Valve Column Cover

Column Holder
FROM COLUMN Connector

・Replace the solvent which is able to use with the replaced column(s), with
before used solvent, thoroughly in the flow line of LC-92XX.
Before the solvent replacement, test the solubility between replaced solvent
and before used solvent.
Use Auto-cleaning function for the solvent replacement in the LC.

・Connect the pipe on the [TO COLUMN connector].

Do not connect the pipe on the replaced column(s) yet.

・Set the pump flow rate to use with the replaced column(s).

4-2
 ・Cover the outlet of the pipe with a paper towel or thick paper for absorption of
the over flowed solvent.
And start the pump for purging air in the pipe. Please make sure to protect
the replaced column(s).
If the flow rate is large, solvent may flush and spray. Be careful.
Thick Paper for Absorption

Be careful of the
spray.

TO COLUMN Connector

・Once stop pump and remove the seals of the replaced column(s). Connect
the pipe on inlet of the replaced column(s).

・The pipe is connected on [TO COLUMN connector].

Keep the flow of the replaced column(s) according arrow indicator [Flow
seal] on the column. The flow of the replaced column(s) is important for the
column performance. Do not connect the reverse direction.
FLOW

Column Column
Inlet ↑ Outlet

FLOW Seal

・When the column is jointed series two or more, the air in the jumper pipe
jointed columns also should be purged.

・Joint the jumper pipe on the first column outlet. Do not joint the pipe on the
second column inlet on this point.

・Cover the outlet of the jumper pipe with a paper towel or thick paper for
absorption of the over flowed solvent. And start the pump for purging air in
the pipe. If the flow rate is large, solvent may flush and spray. Be careful.

Column Outlet

Thick Paper for Absorption

Be careful of the
spray.

・Stop the pump.

4-3
・Connect the jumper pipe on the second column inlet. Connect the pipe from
the column outlet on the [FROM COLUMN connector].

From Column Outlet

FROM COLUMN
Connector

・Make sure that no leakage is found when the pump is started, then close the
front door.

Pipes for LC-9210 NEXT

Parts No. Name
911-001 Inlet pipe for Preparative Column
Outlet pipe for Preparative Column
911-002
(twin column)
Outlet pipe for Preparative Column
911-003
(single column)
911-004 Jumper pipe for Preparative Columns
Pipes for LC-9225 NEXT
Parts No. Name
913-001 Inlet pipe for Preparative Column
Outlet pipe for Preparative Column
913-002
(twin column)
Outlet pipe for Preparative Column
913-003
(single column)
913-004 Jumper pipe for Preparative Columns

4-4
<Removing Column>
To remove columns, refer to the instructions below.

・Turn the column reject valve to the [REJECT] position.

・Remove the LC-92XX NEXT column cover (see page 4-2).

・Remove the outlet pipe column. When the Column pressure is high, you
may have dispersed solvent. Be careful.

Remove the column outlet pipe.

Caution the dispersed solvent
after removing.
To FROM COLUMN Connector

・Put the seal plug into the column outlet. Tighten the seal plug 1/4 turns by
hand, and then tighten by a wrench. Use a hand for a PEEK-type seal
plug. Too strong tightening may damage the screws or column. Be
careful.

The tip is a pin

type

Seal Plug

・Disconnect the column inlet pipe.

・Put the seal plug into the column inlet.

Make sure that the column removed the pipe are tightly sealed without
solvent leakage, keep in cool dark place.

・Connect with the U shape pipe between the [TO COLUMN connector]
and [FROM COLUMN connector] of the LC that are removed the pipe
from the Column.

・Close the column cover.

LC-92XX NEXT common part

Parts No. Name
901-024 Seal plug : for 1.6φ pipe (column seal)

4-5
Cleaning of Solvent Filter
When the pump flow rate is not stable and, when no solvent is supplied at the auto-
cleaning, the Solvent filter may cause clogging of dust or particles.

The Solvent filter is clogged with dirt and dust if the solvent without dust removal is fed
to the solvent reservoir.

<Cleaning Solvent filter>

・Open the front door. Remove the solvent filter connected on the solvent
supply port.
Front Door Solvent Supply Port

Solvent Filter

・Remove the Solvent filter and pipe from the solvent reservoir, put the
Solvent filter and pipe into a beaker filling the solvent.

・Do clean for 10 minutes with the beaker dipped into an ultrasonic cleaner.

・Replace the filter pipe on the solvent supply port.

LC-92XX NEXT common part

Parts No. Name
995-017 Solvent supply connector (for NEXT)
995-018 Gallons bottle pipe (for NEXT)

4-6
Maintenance of Manual Injector
After high concentration or low solubility sample injection, the Stator face, and the
Rotor seal of the Injector may dirt or precipitate with the remained sample.
If you do not wash with the needle port cleaner after the injection the symptom may
occur. If solvent start leaking from Manual Injector, or if the ghost peaks appear,
Manual Injector may be improved by maintenance.
Rotor seal and Stator face should be replaced every year.

<Removing Manual Sample Injector>

・Turn the column reject valve to the [REJECT] position.

・Remove the Solvent filter and pipe from the solvent reservoir.

・Remove the column cover of the LC-92XX NEXT from the chassis.

・Remove pipes marked 2, 3, 5, 6 (marked by RHEODYNE Co.) from the

back of a Manual Injector.

・Disconnect the connector of start signal cable.

1, 4 are connected a Sample loop, and no need of removal during the
maintenance.

2 1

3 6: RHEODYNE Mark

Start Signal Cable

4 5

・Loosen two socket set screws side of the manual injector knob at the
front of the LC-92XX NEXT with a hexagonal wrench.

Injector Knob (front)

Socket Set Screws

・Remove the manual injector knob.

4-7
・Remove retaining screws of the Manual Injector.

Retaining Screws of the Manual Injector

・Move apart the Manual Injector from the back of the LC panel.

<Replacing Rotor Seal and Stator Face>

・Remove Stator retaining screws. Remove Rotor seal, Stator ring, Stator
face, and Stator face from the Manual Injector body.
Stator Ring

Stator

Stator Retaining
Screws

Rotor Seal

Stator Face

・Install New Rotor seal. Place the Rotor seal to fit the tip of the needle port
tube into the liquid passing hole, and insert pins into the pin holes. Insert
as the mark of RHEODYNE co. on the Rotor seal faced toward Stator
face.

・Install Stator face as 3 pins fit on each hales.

・Insert Stator ring into the Manual Injector body.

・Insert pins of Stator ring fit on the grooves.

・Tighten retaining screws of the Manual Injector tightly.

4-8
<Installing Manual Sample Injector>

・Place the Manual Injector on the original position at the back of the LC
panel, and tighten the Manual Injector by retaining screws.

・Replace and tighten the Manual Injector knob. Check the smooth
movement of Sample injection.

・If the movement of the Manual Injector knob is too hard or too loose,
readjust the Manual Injector knob. Loosen two socket set screws side of
the knob again. Readjust and insert the knob as fit perfectly the key into
the groove of the knob.

Key of the Knob

Groove of the Knob

・Readjust the knob to turn. Appropriately check the movement of the knob
and readjust.

・Tighten the retaining socket set screws to install the Manual Injector knob.

・Connect each pipes restored on the back connection port of the Manual
Injector.

・Connect the connector of start signal cable.

・Start Pump and flow solvent using Auto-cleaning function, and check that
no leakage is appeared at any connector. If the leakage is found, tighten
the connector additionally to stop.

・After the Auto-cleaning, Start Pump and flow solvent into the Column to
pressurize the system and check that no leakage is appeared at any
connector.

・Close the column cover.

LC-92XX NEXT common part

Parts No. Name
996-012 Variable Injector(7725i)
996-014 Gauge needle for Variable Injector
996-015 Rotor seal for Variable Injector
996-016 Stator face Assembly for Injector

4-9
Maintenance of Pump
The pump has the necessary cleaning supplies and replacement parts, after which
period of time.
The pump seal replaced every four months, do cleaning the pump head in same time.

The check valve packing to exchange every six months, please replace with new ones
every two years.
Around the time of consumption may be due to the property and frequency of the
sample.

<Replacing Pump Seal>

・Turn the column reject valve to the [REJECT] position.

・Remove the Solvent filter and pipe from the solvent reservoir.

・Open the LC-92XX NEXT front door.

Pump Unit

Front Door

・Pump unit is installed in the upper left in the body.

・Disconnect the pipes A, B, C, D connected to the pump unit. For the pipes B
and C, remove the pipes while fixing the check valve cases with a wrench.

While fixing the check valve case with a

10 mm wrench, remove the upper B C
screw with a 8 mm wrench.

Pump Head
Turn by hand.
D
If the screw is hard, A
cover the screw with
paper and turn by a
nipper.

4-10
 ・Loosen the screw of the pump head with a 2.5 mm hexagonal wrench.
Loosen the screws alternately.

・Pull the pump head. Pull horizontally on the front. The tilted pull may damage
the plunger. Be careful.
Loosen the screws
alternately.

・Remove the pump seal G.

Remove the pump seal G by holding the flange part by hand.
Remove the backup ring left in the main body of the pump unit by holding it
with tweezers (you should also replace the backup ring).

Pump Seal G

Backup Ring
(remove with tweezers.)

・Loosen the screw of the pump head guide with a 2.5 mm hexagonal wrench.
Loosen the screws alternately.
・Pull the pump head guide.
*Internal parts may fall out. Be careful not to lose them, especially the
stainless ball.

Pump Head Guide

Stainless Ball
Loosen the screws (keeps on the far
alternately. side with grease.)

4-11
・ Remove the pump seal F attached to the pump head guide by using the seal
removal jig.
Seal Removal
Jig
Use the side with
screw.

Pump Seal F

・Clean the pump head. Put Pump head into the beaker. The most frequently
used solvent fills the beaker. Make ultrasonic cleaning for 5 minutes. Empty
the solvent in the beaker. Fill a beaker of methanol. Make ultrasonic cleaning
for 5 minutes. When methanol is non-miscible with used solvent, use ethanol
instead of methanol. Wash the Pump head attached the check valve without
any problems.
・Also wipe around the plunger with BEMCOT or soft gauze. In severe cases
of contaminated, moisten the gauze with a strong solvent like as HFIP and
wipe the Plunger with the moistened gauze.

・Install the pump seal F on the pump head guide by using the seal removal jig.
Seal Removal Jig

Use the side without

screw.

Keep the pump head

straight to Pump Seal holes
for mounting.

・Install the pump head guide on the pump unit. Insert the pump head guide
horizontally, being careful not to damage the pump seal F with the plunger.
・Tighten the screws of the pump head guide lightly. Tighten two screws on the
pump head alternately by 1/4 turns with a 2.5 mm hexagonal wrench to
tightly fix it.
Install the pump head guide with its holes facing outward.
Hole

Hole

4-12
 ・Insert the backup ring into the plunger.

・Install the pump seal G on the pump head.

・Tighten the screws of the pump head lightly. Tighten two screws on the pump
head alternately by 1/4 turns with a 2.5 mm hexagonal wrench to tightly fix it.
Be careful not to turn upside down.

・ Connect the pipes A, B, C, D on the pump head. For the pipes B and C,
connect them while fixing the check valve cases with a wrench.

While fixing the check valve case with a

10 mm wrench, tighten the upper screw B C
with a 8 mm wrench.

A D

・Put the Solvent filter into the solvent reservoir. Fill the Pump head with
the solvent to use the [AUTO CLEAN] operation.

・Make sure no leakage. Close the front door.

4-13
<Cleaning Check Valve, Replacing Check Valve Seal>

・Turn the column reject valve to the [REJECT] position.

・Remove the Solvent filter and pipe from the solvent reservoir.

・Open the LC-92XX NEXT front door.

Pump Unit

Front Door

・Pump unit is installed in the upper left in the body.

・Disconnect the pipes A, B connected to the pump unit. For the pipe B,
remove the pipe while fixing the check valve case with a wrench.

While fixing the check valve case with a

10 mm wrench, remove the upper screw B
with a 8 mm wrench.

Turn by hand.
If the screw is hard, cover A
the screw with paper and
turn by a nipper.

・Remove the check valve inlet case and the check valve outlet case from the
pump head. Loosen the check valve to turn anti-clockwise. Be careful not to
lose inside parts. Remove the check valve outlet to turn anti-clockwise.
Check Valve Outlet Case

Check Valve Inlet Case

4-14
 ・The inside parts of the check valve is shown as following figure. Handle as a
unit each as "check valve case", "seat", and "check ball", do not confuse the
parts each other. Place each unit separately in the beakers.

Check Valve Seat B

Check Valve Seat A

Check Valve Seat A

Check Valve Seat B

Check Valve Inlet Case Check Valve Outlet Case

・Fill each beakers of methanol. Make ultrasonic cleaning for 5 minutes.

・If dirt remains in the visual, remove the dirt with chloroform, toluene, or HFIP
as strong solvent.

・Assemble the check valve. Assemble the check valve as shown below.
Check Ball
Check Valve Case

Flow direction

Seat (Glossy surface)

Assemble glossy surface to a
check ball seat side. Assemble
Seat (Frosted surface)
frosted surface to outside.

・Assemble the check valve inlet. Assemble I part on the on the paper. Cover
gently Assemble part I with the check valve inlet case. Turn over gently by
pinching the corners of the paper.
Cover gently Assemble
part 1 with the check
valve inlet case.

Ⅰ Turn over gently by Low hexagon

pinching the corners head type (4 mm)
Check Valve Seat A of the paper.
Seat
Check Ball
Check Valve Case
Check Valve Seat B
Paper

4-15
 ・Assemble the check valve outlet. Assemble the check valve Ⅱon the paper,
holding your finger on the top of the parts, and turn over assembled check
valve gently by pinching the corners of the paper. Put the assembled parts
gently in the check valve case. Turn over the Cover with the assembled parts.
Turn over gently
by pinching the
Turn over gently by corners of the
Ⅱ
pinching the corners paper.
of the paper.
Check Valve Seat B
Seat
Check Ball
Check Valve Case
Check Valve Seat A
Paper

Turn over gently by

pinching the corners
of the paper.

High hexagon
head type (7
mm)

・Connect the check valve outlet case into the pump head inlet case, and
tighten the case with a 10 mm wrench. The gasket B is fixed tightly into the
case. Remove the case, and make sure that the check valve gasket B
insertion is tight. Then connect the case to the pump head outlet side and
tighten it with a 10 mm wrench.
Connect the check valve outlet Connect the case
case into the pump head inlet into the outlet side,
side, tighten the case with a and fix it with a 10
10 mm wrench, and then mm wrench.
remove the case.

Remove the case, and make

sure that check valve seat B
insertion is tight and any parts
not fall.

・ Connect the check valve inlet case into the pump head inlet and tighten by a
wrench anchor.
Connect the case into
the inlet side, and fix it
with a 10 mm wrench.

4-16
・The pipe B is connected to the pump unit as before. Tighten the screw of
pipe B. The Check valve outlet case is fixed by a wrench while the screw is
tightening.

Fix the check valve case with a 10 mm

wrench, and tighten the screw. B

Tighten the screw

firmly by hand. A

・Use Auto-cleaning function for the solvent replacement in the Pump head.

・Make sure that no leakage is found. Close the front door.

Parts of LC-9210 NEXT (FX-10)

Parts No. Name
918-312 Pump seal G
918-013 Check valve inlet (Set)
918-014 Check valve outlet (Set)
918-217 Check valve
918-218 Valve seat case
918-219 Check valve seal A
918-220 Check valve seal B
918-011 Plunger
Parts of LC-9225 NEXT (FX-25)
Parts No. Name
928-312 Pump seal G
918-013 Check valve inlet (Set)
918-014 Check valve outlet (Set)
918-217 Check valve
918-218 Valve seat case
918-219 Check valve seal A
918-220 Check valve seal B
928-011 Plunger

4-17
Pipe Connection
Attached standard pipe PEEK and Stainless steel pipe is a consumable.
Repeated connection and release of Ferrule will be occurred collapse or bite of the
screws. Increased dead volume and leakage or damage of the screws may cause.
Replace with new ones when the connector repeats three times of lock and release.
PEEK pipe has life. If only organic solvent is used, PEEK pipe early decays. Solvent
leakage or splash may occur from the decayed PEEK pipe.
Please replace a new one a month in continuous operation.

<Pipe and Connector, Name and Shape>

A) Male screw B) Back ferrule

C) Front ferrule D) Fitting for PEEK pipe

<Pipe Line Connections>

・Insert a Male Screw, a Back ferrule, and a Front ferrule into the pipe.

Front Ferrule

Back Ferrule

Male Screw

In case of PEEK pipe, insert only D) Fitting for PEEK pipe into the pipe.

4-18
・Insert top of the pipe into a union connector. Pressing the pipe into the
bottom of the connector, turn clockwise by hand.
・ Pressing the pipe into the bottom of the connector, turn 3/4 clockwise by a
wrench.
Union
Turn 3/4 clockwise by a
wrench

Pressing the pipe into the

bottom of the connector

In case of PEEK pipe, turn clockwise Fitting for PEEK pipe only by hand, and
do not use a wrench or other tool.

901-010 Nut for 1.6φ pipe

901-011 Ferrule for 1.6φ pipe (pair)
901-023 Male Screw for 1.6φ pipe
901-030 SUS 1.6φ pipe
901-075 PEEK pipe (o.d.1.5mm, i.d. 0.5mm)
901-076 PEEK pipe (o.d.1.5mm, i.d. 0.8mm)
901-077 Fitting for PEEK pipe

4-19
Adjustment of RI Detector
Optical zero of RI Detector may shift according solvent to use. After turning the power
switch on, the output value that is displayed on the LCD display immediately after the
auto-cleaning operation indicates the optical position. If the monitoring output is over
±200, the optical alignment is recommended with the re-alignment of the NUL glass.

<Optical Alignment of RI Detector>

・Turning power switch on.

・Press the [CLEAN UP] key to execute the auto-cleaning.

・After the [CLEAN UP] operation, check the RI output value on the LCD display.

・ Remove the Column cover of LC-92XX NEXT from the chassis.

Null Glass Adj. Hole

Column Cover

・Insert a blade of a screw driver and turn with slot of the alignment screw.

・While checking the RI output value on the LCD display of the main body, turn
the driver to perform zero adjustment.

・Remove the driver from the hole. Close the Column cover.

4-20
Manual Cleaning Instruction of Buffer Volume
The LC-92XX series has the Reverse flow protection during Recycling Separation as a
standard. The Reverse flow protection system has a buffer-volume.
The buffer-volume is automatically cleaned in the CLEAN UP operation. When the LC-
92XX has stopped in long term, or when the high-viscosity or low solubility sample is
injected, the buffer-volume is contaminated and cannot be cleaned with the CLEAN
UP operation.
If the noise from the buffer volume contamination is remarkable, following Manual
Cleaning of the buffer volume is recommended.

<Manual Cleaning of Buffer Volume>

・Clean the buffer volume with the CLEAN UP operation.

・After the CLEAN UP operation, turn the Pump Drain Valve to the [DRAIN].

・Turn the Column reject valve in the middle between [REJECT] position and
[CONNECT] position.
(Refer to below figure.)

REJECT

Turn the valve in the middle between

[REJECT] and [CONNECT].

CONNECT

・Set the flow rate to maximum. Set 10 mL/min for LC-9210 NEXT and 25
mL/min for LC-9225 NEXT.

・Start the pump, and check that the solvent flows steadily from the pump drain
out in the siphon box. If the flow from the Pump Drain is not appeared, stop
the pump as soon as possible. If setting is abnormal in the Pump Drain Valve
or the Column reject valve, the maximum flow may damage the column.
Check the position of two valves, and re-start the pump.

・Press the key in [RECYCLE].

・One minute later, press , and then stop the pump to finish the cleaning
maintenance.

4-21
 4-2

Chapter 2 Trouble Shooting

LC-92XX NEXT series mechanical failure, error message and remedy

4-22
Chromatogram Acquisition Error
If chromatogram is abnormal, refer to following paragraph and to remedy.
If abnormal chromatograph cannot be remedy, please contact us or our representative
at the end of the book.

<Base Line Wander 1>

！Babble in the Reference cell of RI Detector!

・Solvent degassing is recommended.

For details on degassing, refer to the pages describing degassing in
"General Information Manual".
Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium.
Check the base line again.
If the wander cannot be remedy, go to the next step <Base Line Wander 2>.

4-23
<Base Line Wander 2>

！Babble in the Sample cell of RI Detector, babble in the eluent!

・Check the solvent filter line.

If the babble is found in the solvent filter line, use degassed solvent for the
eluent.
For details on degassing, refer to the page 2-48 describing degassing in
"General Information Manual".

Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium. Check the base line again.

！Leakage of the UV Detector cell!

・Please contact us or our representative at the end of the book.

！Decay of UV Lamp!

・Please contact us or our representative at the end of the book.

！Leakage of the flow line!

・Check the connectors of pump-injector-column-detector.

If the leakage at the connection of flow is found, tighten the screw further,
or replace the new flow line.

4-24
！Loose connection of the Signal Cable!

・Check the connection of the Signal Cable.

If the Loosen connection is found, re-tighten the connection in proper.

！Decay of Pump seal!

・Check the pump head.

If solvent leakage is found under the pump head, refer to Maintenance of
Pump the page 4-10 of "Maintenance Manual" (this manual) for replacement
of the pump seal to replace the pump seal if need.

4-25
<Base Line Drift>

！Environmental influence of the Laboratory!

・Check that wind hit the LC chassis directly, or that there is a temperature
change in the laboratory.
RI Detector is sensitive to fluctuation of temperature.
Fluctuation of temperature may cause the base line drift of the RI Detector.

！RI Detector temperature controller works not enough!

・Make sure that the time is over 30 to 100 min after the CLEAN UP operation.
Wait that the temperature controller become stable.

！Solvent replacement is not enough!

・Wait that the Column become stable.

！Contamination of the column is continued!

・Column cleaning is required.

Refer to the column instruction and clean the column if need. In case of
JAIGEL-H series, the solvent replacement is impossible. For the JAIGEL-H
series, the addition of 0.1 - 0.5% tri-ethylamine in the solvent is
recommended. The addition removes and purges the contamination on the
packing material.
In case of using Chloroform, the addition of tri-ethylamine hydrochloride is
recommended in concentration of 50 mM/L.
Before cleaning, test that the sample does not re-precipitate in the added
solvent, and then do the column cleaning with the solvent.

4-26
！Leakage of the flow line!

・Check the connectors of pump-injector-column-detector.

If the leakage at the connection of flow is found, tighten the screw further,
or replace the new flow line.

！Decay of Pump seal!

・Check the pump head.

If solvent leakage is found under the pump head, refer to Maintenance of
PumpMaintenance of Pump the page 4-10 of "Maintenance Manual" (this
manual) for replacement of the pump seal to replace the pump seal.

！Leakage of the UV Detector cell!

・Please contact us or our representative at the end of this manual.

！Decay of UV Lamp!

・Please contact us or our representative at the end of the book.

！Contamination of the RI cell!

・Make sure that the UV Detector signal has no noise.

If base line drift cannot be remedied yet, RI detector cell might be dirty.
The RI detector cell is built-in. Customers cannot disassemble and wash the
RI detector cell. Please contact us or our representative at the end of the
book.

4-27
<Base Line Drift during Recycling Operation>

0 50 Rt (min) 0 50 100 Rt (min)

！Contamination of the buffer volume!

・Clean the buffer volume with the CLEAN UP operation.

When the LC-92XX is started and finished, clean the buffer volume with the
CLEAN UP operation.
When the CLEAN UP is neglected, the base line drift may occur from the
second-cycle of the recycle operation.

！Not suitable selection of the column!

・When the selection of the column against the sample is not suitable, the base
line is drifting upward in the Recycling operation. Select suitable column(s)
for the sample.

4-28
<Poor Recycling Efficiency>

0 20 40 Rt (min) 0 20 40 60 Rt (min)
(Normal) (Poor)

！Diffusion of the Recycled Sample in the Sample loop!

・When multiple times Recycling is done, turn over the knob to LOAD to
eliminate the sample loop from the flow line because the recycled sample
diffuse in the sample loop.
When you want to use the manual sample injector to execute the recycling
separation, flow the solvent of 3 mL or more for LC-9210 NEXT or 10 mL or
more for LC-9225 NEXT after the sample injection, and then turn the manual
injector knob to the [LOAD] position.

！Not suitable separation conditions!

・When the selection of the column and solvent against the sample is not
suitable, Poor Recycling efficiency appears in the Recycling operation.
Select suitable column(s) and solvent for the sample.

4-29
Abnormal Pressure
Abnormal pressure may occur from decayed flow parts, sample property, or handling
error. Refer to following paragraph and to remedy the abnormal pressure.
When the abnormal pressure cannot be remedy, please contact us or our
representative at the end of the book.

<Abnormal High Pressure>

・If the maximum pressure limit is set, an error message screen appears to
inform the emergency stop of the pump.
Check that the [MAXMAM] pressure limit is set to monitoring pressure +
2.0 MPa.

！Clogging of the Column or Dirt of the Column Packing!

・Press the [OK] key, and turn the column reject valve to [REJECT] to start the
pump.
・If the pressure is significant smaller than before, column clogging may occur.
・Stop the pump.
・Remove the column from the flow line. Turn the Column reject valve to
[CONNECT] position. Re-start the Pump and check the pressure.
・If the pressure is significant large without the Column, the clogging may be
between the LC chassis and the Column inlet. Replace flow line part
between the LC chassis and the Column inlet.
・If the pressure is abnormally high with connection of the Column,
replacement of the Column is need.

！High viscosity of the solvent!

・In case using reverse phase column or using high polarity column, the
solvent replacement of the column may occur high pressure. While the
solvent replacement is finished, set the maximum limiter at the suitable value.

4-30
<Abnormal Pressure Fluctuation>

・If the monitoring pressure abnormally fluctuates, the pressure fluctuation may
occur the abnormal chromatogram or decay of the column. Stop the Pump,
check the following items.

！Clogging of the check valve!

・Turn the pump drain valve to [DRAIN].

・Set the flow rate to 5 mL/min for LC-9210 NEXT and 15 mL/min for LC-9225
NEXT, and start the pump.
・Check that the solvent flows steadily from the pump drain out in the siphon
box. If the flow from the Pump Drain is not constant or repeat stop and flow,
the check valve cleaning is required. Refer to the check valve Cleaning at 4-
14page in this manual.

！Leakage of the flow line!

・Check the connectors of pump-injector-column-detector.

If the leakage at the connection of flow is found, tighten the screw further,
or replace the new flow line.

！Decay of Pump seal!

・Check the pump head.

If solvent leakage is found under the pump head, refer to Maintenance of
Pump the page 4-10 of "Maintenance Manual" (this manual) for replacement
of the pump seal to replace the pump seal.

4-31
！Babble in the Sample cell of RI Detector, babble in the eluent!

・Check the solvent filter.

If the babble is found in the solvent filter line, use degassed solvent for the
eluent.
For details on degassing, refer to the page 2-48 describing degassing in
"General Information Manual".
Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium. Check the base line again.

4-32
<Abnormal Low Pressure>

・If the minimum pressure limit is set, an error message screen appears to
inform the emergency stop of the pump.
Check that the [MINIMAM] pressure limit is set to monitoring pressure - 2
MPa or less.

！Babble in the Sample cell of RI Detector, babble in the eluent!

・Check the solvent filter.

If the babble is found in the solvent filter line, use degassed solvent for the
eluent.
For details on degassing, refer to the page 2-48 describing degassing in
"General Information Manual".

Do the CLEAN UP operation with degassed solvent and wait that base line
becomes stable after the column equilibrium. Check the base line again.

！Clogging of the check valve!

・Turn the pump drain valve to [DRAIN].

・Set the flow rate to 5 mL/min for LC-9210 NEXT and 15 mL/min for LC-9225
NEXT, and start the pump.
・Check that the solvent flows steadily from the pump drain out in the siphon
box. If the flow from the Pump Drain is not constant or repeat stop and flow,
the check valve cleaning is required. Refer to the check valve Cleaning at 4-
14page in this manual.

！Solvent loss in the Solvent container!

・Make sure that Solvent fills enough in the Solvent container.

・If the Solvent is lost in the container, the Pump occurs emergency stop to
prevent air introduction into the column.

4-33
！Leakage of the flow line!

・Check the connectors of pump-injector-column-detector.

If the leakage at the connection of flow is found, tighten the screw further,
or replace the new flow line.

4-34
Abnormal Signal
The decay of the consumable parts or the property and miss-operation, may occur the
abnormal signal of the Detector.
When the abnormal signal cannot be remedy, please contact us or our representative
at the end of the book.

<Fail Power>

！Disconnection of POWER Cable!

・Make sure the insertion of the POWER Cable at the rear of LC chassis.

<No Signal and No Event Mark>

！Disconnection of SIGNAL Cable!

・Make sure that the connection of signal cable on the Output terminal block at
the left hand of LC.
Check the input cable connection as same.

！Failure of the Recorder or Integrator!

・Please carefully read the instruction manual for your attachment.

4-35
<UV Detector Abnormal Signal>

！Dirt in the UV Detector cell and/or Leakage of the cell!

・If the solvent leaks from the UV detector cell, the chromatogram may cause
fluctuation of base line and the sensitivity loss may cause. In the Recycling
separation, extreme low peaks may appear with the leakage.
If above chromatogram is obtained, please contact us or our representative
at the end of the book.

！Decayed UV Lamp!

・If the UV Lamp decays, the chromatogram may cause fluctuation of base line.
The zero balance of the baseline may not achieve. or the sensitivity loss may
cause. If above symptoms appear, please contact us or our representative at
the end of the book.

！Selected UV wave length is not suitable for the sample!

・Each sample has suitable UV wave length for detection.

Select suitable UV wave length for the sample.

<RI Detector Abnormal Signal>

！Babble in the Reference cell of RI Detector!

・Solvent degassing is recommended.

For details on degassing, refer to the page 2-110 describing degassing in
"General Information Manual". Do the CLEAN UP operation with degassed
solvent and wait that base line becomes stable after the column equilibrium.
Check the base line again.

！RI Detector is out of optical alignment!

・If RI Detector is extremely out of optical alignment, electrical zero may not
achieve or the RI signal causes full- scale in tight range.
Refer to p.4-20 described RI Detector Adjustment, and do re-alignment of RI
Detector.

4-36
Error Message
If a failure or wrong operation occurs in the LC-92XX NEXT, an error message
appears on the LCD display. At the beginning of the error message, "ERROR" is
displayed followed by a number. Based on this number, perform the following
operations to resolve the cause of failure. If the error message appears again, please
contact us or our representative at the end of the book.

<ERROR 01>

< E R ROR 0 1 >

PRE SS VA L UE
M A X I M UM < M I N I M UM
PUSH "OK " KE Y

！This error occurs if the minimum pressure limit is set to a value larger than
the maximum pressure limit when setting them.

・Set them so that the maximum pressure limit is larger than the minimum
pressure limit.

<ERROR 02>

< E R ROR 0 2 >

PRE SS VA L UE
M A X I M UM = M I N I M UM
PUSH "OK " KE Y

！This error occurs if the maximum pressure limit is set to a value equal to the
minimum pressure limit when setting them.

・Set them again so that the maximum pressure limit is not equal to the
minimum pressure limit.

4-37
<ERROR 03>

< E R ROR 0 3 >

P U R G E MO D E
( WA I T 6 0 S E COND )
PUSH "OK " KE Y

！This error occurs if you press one of the [Co/Dr] key in [FRACTION], the
[START] key in [CLEAN UP], the [ON/OFF] key in [RECYCLE], and the
[FLOW SET] key in [PUMP], during the [PURGE] operation of [RI
DETECTOR].

・These keys are unavailable during the [PURGE] operation. Use them after
the [PURGE] operation.

<ERROR 04>

< E R ROR 0 4 >

R E C Y C L E O N MO D E
( CHANGE R E CYC L E O F F )
PUSH "OK " KE Y

！This error occurs if you press one of the [START] key in [CLEAN UP], the
[PURGE] key in [RI DETECTOR], and the [FLOW SET] key in [PUMP],
during the [RECYCLE] operation.

・These keys are unavailable during the [RECYCLE] operation. Use them after
the [RECYCLE] operation.

4-38
<ERROR 05>

< E R ROR 0 5 >

P UM P F L OW L I M I T

PUSH "OK " KEY

！This error occurs if you set a value greater than the maximum flow rate of the
pump when changing the flow rate.

・Set a value less than the maximum flow rate of the installed pump.
LC-9210 NEXT(FX-10) : 10.0 mL/min
LC-9225 NEXT(FX-25) : 25.0 mL/min

<ERROR 06>

< E R ROR 0 6 >

C L E A N U P I S NO T MAD E

PUSH "OK " KEY

！This error occurs when normal cleaning is not performed in three minutes
during the buffer tube cleaning of the [CLEAN UP] operation.

・If the error is not solved, please contact us. Followings are possible causes of
the error:
1. The sensitivity of the sensor of the buffer tube is too low.
2. The buffer tube is very dirty.
The error will be solved by replacing these parts.

<ERROR 07>

< E R ROR 0 7 >

B E YOND T H E L I M I T

PUSH "OK " KEY

！This error occurs if you set a value greater than or equal to 50.1 MPa in the
maximum or minimum pressure limit setting screen.

・Set each pressure limit to a value less than 50.0 MPa.

4-39
<ERROR 08>

< E R ROR 0 8 >

T H E P UM P I S
NO T OP E R A T I NG
PUSH "OK " KE Y

！This error occurs if you press the [Co/Dr] key in [FRACTION], the [ON/OFF]
key in [RECYCLE], or the [PURGE] key in [RI DETECTOR] when the pump
is stopped.

・Press the [ON/OFF] key in [PUMP] to start the pump, and then press these
keys.

<ERROR 09>

< E R ROR 0 9 >

T U R N O N T H E P UM P
WH E N U S I NG R E C Y C L E
PUSH "OK " KE Y

！This error occurs if you press the [ON/OFF] key in [PUMP] during the
[RECYCLE] operation.

・Stop the [RECYCLE] operation, and then stop the pump.

*The pump is stopped when ERROR 09 is displayed.

<ERROR 10>

< E R ROR 1 0 >

P R E S S OV E R L I M I T
( CL E AN UP )
PUSH "OK " KE Y

！This error occurs when the pressure exceeds 1.5 MPa during the [CLEAN
UP] operation.

・Check that the column reject valve is located at the [REJECT] position.

4-40
<ERROR 11>

< E R ROR 1 1 >

T U R N O N T H E P UM P
WH E N US I NG I N J E C T I ON
PUSH "OK " KE Y

！This error occurs if you perform the injection operation using the manual
sample injector (INJECT->LOAD->INJECT) when the pump is stopped.

・Start the pump before performing the injection.

<ERROR 12>

< E R ROR 1 2 >

I NVA L I D
OP E RA T I ON
PUSH "OK " KE Y

！This error occurs if you press an irrelevant key when setting a function, or if
you press a key for setting a function when the function is running.

・This error occurs in the following situations:

1. When you press a setting key ([MAXIMUM], [MINIMUM], or [RANGE]),
while fraction collection is being executed by the [Co/Dr] key in
[FRACTION], or while the solvent is running through the dead volume.
2. When you press the [Co/Dr] key in [FRACTION], while you are setting the
function by using the setting keys ([MAXIMUM], [MINIMUM], [RANGE], or
[DEAD VOL SET]).
After the operation finishes or the setting is completed, press the key.

<ERROR 13>

< E R ROR 1 3 >

T H E P UM P I S
OP E RA T I NG
PUSH "OK " KE Y

！This error occurs if you press the [START] key in [CLEAN UP] when the
pump is running.

・Stop the pump, and then press the [START] key in [CLEAN UP].

4-41
<ERROR 14>

< E R ROR 1 4 >

P R E S S OV E R L I M I T
( P UM P )
PUSH "OK " KE Y
< E RROR 17>
BURN
！This error occurs when an abnormality O U Tto the pressure rise of the pump
due
( S T O P T H E P UMP )
is detected. PUSH "OK " KE Y

・As the failure may occur on the parts in the pump, please contact us or our
representative.

<ERROR 15>

< E R ROR 1 5 > < E RROR 1 8 >

OV E R 5 0 . 0 MP a P UMP D O E S
( M A X I M UM S E T V A L U EP)U NS O T ROT A T E
H "OK " KE Y
PUSH "OK " KE Y

！This error occurs when the monitoring pressure exceeds the specified
maximum pressure limit.

・Refer to the page 4-30 of this manual to solve the problem according
<Abnormal High Pressure>. < E R ROR 1 9 >
SOL VE NT E SCAP ED
*The pump is stopped when ERROR(15
C HisE displayed.
C K T H E P UM P )
PUSH "OK " KEY

<ERROR 16>

< E R ROR 1 6 >

F A L L B E L OW 0 1 . 0 M P a
( M I N I M UM S E T V A L U E )
PUSH "OK " KE Y

< E R ROR 20>

！This error occurs when the monitoring
F R A C Tpressure
I O N MO falls
D E below the specified
CO L L E C T → D R A I N
minimum pressure limit. PUSH "OK " KE Y

・Refer to the page 4-33 of this manual to solve the problem according
<Abnormal Low Pressure>.
*The pump is stopped when ERROR 16 is displayed.

< E RROR 2 1 >

B E YOND T H E L I M I T
( M A X M E MO R Y : 3 3 )
PUSH "OK " KE Y
4-42
<ERROR 17>

< E R ROR 1 7 >

B U R NOU T
( S T O P T H E P UM P )
PUSH "OK " KE Y

！This error occurs when the temperature around the pump control board rises
very high (over 60℃).

・Stop the pump for 30 minutes, and then start the pump again. If the error
occurs again, please contact us or our representative.
*The pump is stopped when ERROR 17 is displayed.

<ERROR 18>

< E R ROR 1 8 >

P UM P D O E S
NO T RO T A T E
PUSH "OK " KE Y

！This error occurs if abnormal rotation is detected at the pump driving part
when the pump is running.

・As the failure may occur on the parts in the pump such as board or sensor,
please contact us or our representative.

4-43
<ERROR 19>

< E R ROR 1 9 >

SOL VE NT E SCAP ED
( C H E C K T H E P UM P )
PUSH "OK " KE Y

！This error occurs if leakage is found around the suction pipe of the pump.

・Followings are possible causes of the error:

1. Check that the connectors such as check valve and PEEK screw are
tightened additionally.
2. The leakage is caused by secular change of the pump seal. Refer to the
page 4-10 in this manual to replace the pump seal.
3. Remove the leakage sensor, wipe the liquid out of the sensor, and check
the LED changes from red to green.

If the error is not solved, please contact us or our representative at the end of
the book.

<ERROR 20>

< E R ROR 2 0 >

F R A C T I O N MO D E
CO L L E C T → D R A I N
PUSH "OK " KE Y

！This error occurs, if you press the [PURGE] key in [RI DETECTOR], the
[FLOW SET] key in [PUMP], or the [START] key in [CLEAN UP], while
fraction collection is being executed by the [Co/Dr] key in [FRACTION].

・Press the [Co/Dr] key in [FRACTION] so that the eluent flows from the siphon,
and then press the desired key.

4-44
<ERROR 21>

< E R ROR 2 1 >

B E YOND T H E L I M I T
( M A X M E MO R Y : 3 3 )
PUSH "OK " KE Y

！This error occurs if you press the [Co/Dr] key in [FRACTION] 33 times
continuously.

・If you press the key continuously while the solvent is running through the
dead volume, the press timings are stored, and accordingly the valve works.
33 times is the limit that can be saved while running through the dead
volume. So do not press the [Co/Dr] key continuously over 33 times.

4-45
CUSTOMER SERVICE
If you have any questions, or something is wrong with Recycling Preparative HPLC, please
contact to the following.

208 Musashi, Mizuho, Nishitama, Tokyo 190-1213 Japan

Japan Analytical Industry Co., Ltd
Engineering Department
TEL:+81 42-557-2332
FAX:+81 42-557-0894

Order for Accessories / Supplies

Order for accessories / expendable supplies, please contact to the following.

208 Musashi, Mizuho, Nishitama, Tokyo 190-1213 Japan

Japan Analytical Industry Co., Ltd
Operation Department
TEL: +81 42-557-2331
FAX: +81 42-557-1892

LC-92XX NEXT Series User's Manual (1.3 Edition) Japan Analytical Industry Co.,
January 31, 2012
Ltd.