10.1007 - 978 3 319 14499 3

Soil Biology
K. Sowjanya Sree
Ajit Varma Editors
Biocontrol of
Lepidopteran
Pests
Use of Soil Microbes and their
Metabolites
Soil Biology
Volume 43
Series Editor
Ajit Varma, Amity Institute of Microbial Technology,
Amity University Uttar Pradesh, Noida, UP, India
More information about this series at
http://www.springer.com/series/5138
K. Sowjanya Sree • Ajit Varma
Editors
Biocontrol of Lepidopteran
Pests
Use of Soil Microbes and their Metabolites
Editors
K. Sowjanya Sree Ajit Varma
Amity Institute of Microbial Technology Amity Institute of Microbial Technology
Amity University Uttar Pradesh Amity University Uttar Pradesh
Noida Noida
Uttar Pradesh Uttar Pradesh
India India
ISSN 1613-3382 ISSN 2196-4831 (electronic)

Soil Biology
ISBN 978-3-319-14498-6 ISBN 978-3-319-14499-3 (eBook)
DOI 10.1007/978-3-319-14499-3
Library of Congress Control Number: 2015933012
Springer Cham Heidelberg New York Dordrecht London

© Springer International Publishing Switzerland 2015
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part
of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations,
recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or
information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar
methodology now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this
publication does not imply, even in the absence of a specific statement, that such names are exempt
from the relevant protective laws and regulations and therefore free for general use.
The publisher, the authors and the editors are safe to assume that the advice and information in this book
are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the
editors give a warranty, express or implied, with respect to the material contained herein or for any errors
or omissions that may have been made.
Printed on acid-free paper
Springer International Publishing AG Switzerland is part of Springer Science+Business Media

(www.springer.com)
Foreword
Insect pest management has always been a major concern to farmers around the
globe. Insect pest attack accounts to as high as 42 % loss of crop productivity, as
estimated already a decade back. Lepidopteran pests contribute a major share to this
crop loss. Larvae of these pests are very voracious especially at the younger instars
causing a drastic damage to the crop foliage and produce. Although their natural
enemies do exist, the man-made ecological imbalances do not allow them to
substantially control the huge pest populations. For a long time, the effective
management of these pests was relied upon the chemical and synthetic pesticides.
But for over half a century now, it has been realised that the indiscriminate use of
these chemicals cause dramatic ill-effects concerning health and environmental
safety. This led to a paradigm shift in the pest management strategies being
practiced.
The development and use of natural enemies including parasites, predators and
pathogens of insect pests as biopesticides have gained impetus in the last few
decades, and biopesticide has become one of the key components of Integrated
Pest Management (IPM). In fact, there are some very successful cases of the use of
biopesticides for insect control. A few instances are the control of sugarcane stem
borer in India with its parasite Trichogramma spp., and saving of the staple food
crop Cassava in Africa from mealybugs by aerial spray of a tiny parasitic wasp,
Anagyrus lopezi. Amongst these biocontrol agents, the entomopathogenic micro-
organisms have had an edge over the others, because of their production and
application feasibility, specificity, economic viability and environmental safety.
Therefore, the promotion of biopesticides for insect control holds great promise for
the future.
The editors of this volume together with the authors of the individual chapters
have made a remarkable contribution in collating the up-to-date information on the
successful development and use of insect pathogenic viruses, bacteria, fungi and
nematodes as biopesticides against the lepidopteran pests. Apart from the use of
v
vi Foreword
these microorganisms, their metabolites have also been screened for use as
biopesticides. The use of transgenic technology in developing pest resistant crop
varieties has led to a completely new era of pest management science. The
overwhelming response of farmers towards the cultivation of Bt-cotton crop is
inspirational in looking forward to more such transgenic resistant varieties in the
near future.
I wish to congratulate the editors for bringing out a unique volume and insight
into biological control of insect pests. This book will be indispensible for scientists
and professionals working in the field of crop protection and can also serve as a
reference book for graduate students.
February 2015 M.V. Rajam

Department of Genetics
University of Delhi South Campus
New Delhi
India
Preface
Lepidopteran pest infestation of economically important plants is a menace to

farmers all over the world. These pests are in general polyphagous and voracious
feeders. The larval stages cause huge economic losses to the agricultural yield.
Although a number of chemical pesticides have been discovered and are in use, the
increasing pest resistance to these chemicals is an alarming problem in addition to
the environmental pollution caused by these chemical pesticides. The use of
biocontrol agents for the control of lepidopteran pests is increasing over time and
could be looked upon as an upcoming solution to resolve the environmental issues.
The entomopathogenic viruses, bacteria, fungi, nematodes and their metabolites
show active insecticidal properties against these pests. This volume, comprising of
16 chapters, is focused on the characterization, mechanism of action and applica-
tion of some of these entomopathogenic viruses, bacteria, fungi, nematodes and the
metabolites of these microbes against the devastating lepidopteran pest species
around the world. The larva of Helicoverpa armigera, one of the economically
important lepidopteran crop pests, infected by NucleoPolyhedro Virus (NPV) is
depicted in Fig. 2.4 (see Chap. 2 of this volume).
Having a focus on different aspects of entomopathogenic soil microorganisms
and their metabolites for their use in biocontrol of lepidopteran pests, this volume
will attract the attention of life science researchers in general and microbiologists,
agricultural researchers, entomologists and applied chemists in specific. It will also
serve to motivate the researchers focused on integrated pest management studies.
It was our pleasure to interact with all the authors, and we thank them for their
stimulating contributions. We sincerely wish to acknowledge Hanna Hensler-
Fritton and Jutta Lindenborn at Springer, Heidelberg, for their generous assistance
and patience in shaping this volume. K. Sowjanya Sree acknowledges the financial
support from Science and Engineering Research Board, Govt. of India, through the
Fast track Young Scientist scheme.
vii
viii Preface
We are thankful to Dr. Ashok K. Chauhan, Founder President of the Ritnand

Balved Education Foundation (an umbrella organization of Amity Institution),
New Delhi, for his kind support and constant encouragement.
Noida, India K. Sowjanya Sree

Ajit Varma
Contents
1 An Introduction to Entomopathogenic Microorganisms . . . . . . . . . 1

K. Sowjanya Sree and Ajit Varma
2 Role of Nucleopolyhedroviruses (NPVs) in the Management
of Lepidopteran Pests in Asia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
G.V. Ranga Rao, Ch. Sridhar Kumar, K. Sireesha, and P. Lava Kumar
3 Bt Insecticidal Crystal Proteins: Role in Insect Management
and Crop Improvement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
K.Y. Srinivasa Rao, Debasis Pattanayak, and Rohini Sreevathsa
4 Identification and Characterization of Receptors for Insecticidal
Toxins from Bacillus thuringiensis . . . . . . . . . . . . . . . . . . . . . . . . . 71
Ricardo A. Grande-Cano and Isabel Gomez
5 Non-Bt Soil Microbe-Derived Insecticidal Proteins . . . . . . . . . . . . . 89
Leela Alamalakala, Srinivas Parimi, Sandip Dangat, and Bharat R. Char
6 The Management of Spodopteran Pests Using Fungal Pathogens . . . 123
Md. Aslam Khan and Wasim Ahmad
7 Comparative Account of Generalist and Specialist Species
of the Entomopathogenic Fungus, Metarhizium . . . . . . . . . . . . . . . . 161
K. Sowjanya Sree and Hemesh Joshi
8 Non-ribosomal Peptides from Entomogenous Fungi . . . . . . . . . . . . 169
Qiongbo Hu and Tingyan Dong
9 Optimization of the Cyclodepsipeptidic Destruxin Recovery from
Broth Culture of Metarhizium anisopliae and Its Augmentation
by Precursor Supplementation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 207
K. Sowjanya Sree and V. Padmaja
ix
x Contents
10 Beauveria bassiana: Biocontrol Beyond Lepidopteran Pests . . . . . . 219

H.B. Singh, Chetan Keswani, Shatrupa Ray, S.K. Yadav,
S.P. Singh, S. Singh, and B.K. Sarma
11 Biocontrol of Diamondback Moth, Plutella xylostella,
with Beauveria bassiana and Its Metabolites . . . . . . . . . . . . . . . . . . 237
Liande Wang, Minsheng You, and Haichuan Wang
12 Entomopathogens for Cotton Defoliators Management . . . . . . . . . 255
K. Sahayaraj
13 Entomopathogenic Nematodes and Their Bacterial Symbionts
as Lethal Bioagents of Lepidopteran Pests . . . . . . . . . . . . . . . . . . . 273
Sharad Mohan
14 The Management of Helicoverpa Species by Entomopathogenic
Nematodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289
M. Abid Hussain and Wasim Ahmad
15 Sustainability of Entomopathogenic Nematodes Against
Crop Pests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 315
S. Sivaramakrishnan and M. Razia
16 An Overview of Some Culture Collections of Entomopathogenic
Microorganisms in the World . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 329
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335
Chapter 1
An Introduction to Entomopathogenic
Microorganisms
1.1 Introduction
The quality and quantity of the agricultural produce is influenced by a variety of

abiotic and biotic factors. The abiotic factors including drought, temperature
extremes and salinity partner with the biotic factors like weed and pest attack to
enhance their negative impact on the crop yield. In addition, in the recent years,
drastic changes in agricultural landscapes have occurred because of the intensifi-
cation of agricultural systems (Robinson and Sutherland 2002; Bianchi et al. 2006).
The simplified agricultural landscapes because of clearing of lands, up-scaling and
expansion of crop fields have eventually led to a reduction in the occurrence of
natural habitats (Robinson and Sutherland 2002; Benton et al. 2003). This has also
led to the loss of biodiversity with respect to natural pest control (Ives et al. 2000;
Wilby and Thomas 2002; Gurr et al. 2003). Apart from this, the changing climate in
the years to come is predicted to have heavy impact on agriculture ranging from
reduction in availability of fertile land (Easterling et al. 2000) to changes in the life
cycle of plant pests and pathogens (Lonsdale and Gibbs 2002; Turner 2008; Collier
et al. 2008).
Crop pests are one of the major contributors to economic losses in the agriculture
sector (Oerke et al. 1994). Considering the pest population, it is biologically a very
diverse group including pathogenic microbes such as viruses, bacteria or fungi and
parasitic nematodes; invertebrates like insects and molluscs; and some vertebrates.
In the present volume, we will focus on the insect pest species. Insects attack the
crop plants by either herbivory, feeding on their sap or by causing or transmitting
diseases, thereby reducing the crop yields globally. They can also attack the stored
agricultural produce further increasing the damage. Order Lepidoptera of the class:
K.S. Sree (*) • A. Varma

Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida 201303,
Uttar Pradesh, India
e-mail: [email protected]
© Springer International Publishing Switzerland 2015 1

K.S. Sree, A. Varma (eds.), Biocontrol of Lepidopteran Pests, Soil Biology 43,
DOI 10.1007/978-3-319-14499-3_1
2 K.S. Sree and A. Varma
Insecta which includes butterflies and moths is one of the most diverse ones (Krenn
2010) and is the second largest order of insects. Many of the lepidopteran insect
species, both in adult and larval stages, are associated with the plants. Infestation of
a wide range of crop plants like tomato, cotton, maize, cauliflower, cabbage,
chickpea, lentils and so on by the lepidopteran insect species causes huge economic
losses globally. The lepidopteran larvae have biting and chewing type of mouth
parts with well-developed mandibles facilitating them to feed voraciously on the
foliage of the crop plants (Krenn 2010). These are considered to be the most
damaging stages of the lepidopteran insect pests. The adults, although the females
have a high fecundity rate, are floral nectar feeders and have been long established
as pollinators of flowering plants (Kevan and Baker 1983; Fenster et al. 2004).
Chemical insecticides have been used now for years for the control of the
damaging lepidopteran populations in the agricultural fields. However, after the
ill-effects of long-term, indiscriminate use of chemical pesticides and insecticides
on environment, human and animal health have been revealed in the past few
decades (Carson 1962; Klaassen et al. 1996; Meiners and Morriss 2001), the use
and development of alternate methods for the control of insect pest populations
became a necessity. Additionally, the number of insect species gaining resistance to
a variety of chemical insecticides has increased tremendously (Mallet 1989). In this
context, the use of biological control agents (BCAs) is being looked upon as an
environmentally safe insect pest control strategy for sustainable agriculture
worldwide.
1.2 Biological Control
Biological control makes use of living organisms or their products which can
manage the insect pest populations thereby minimising the damage to crop yield,
both in terms of quality and quantity (Bale et al. 2008). The biological control
strategies, in many cases, have been developed by researchers taking examples
from nature. The natural enemies in a given agro-ecosystem play a major role in
suppressing the pest populations in that region (Hajek 2004). In this context, it
might be interesting to describe an instance which the author, KSS, came across. A
progressive farmer, Kongara Ramesh, from the Visakhapatnam district of Andhra
Pradesh, India, made a careful observation of the natural death of lepidopteran
larvae each year with a cottony mass over them. He was curious to know the reason
of their death and if this subject could be used as a biopesticide. With the help of
researchers in this field, they found out that it was a fungal pathogen, Beauveria
bassiana. He later went on to culture this entomopathogenic fungus for use in his
agricultural fields. There exist, for sure, many other such farmers around the world.
Biological control programmes operate worldwide in agriculture and forestry.
The BCAs used in pest management strategies include natural enemies of insect
pests such as predatory insects, parasitoids and parasites. In order to have maximum
returns using environmentally sustainable practices, integrated pest management
1 An Introduction to Entomopathogenic Microorganisms 3
(IPM) strategies are being taken forward which include effective mixtures of
different biological, chemical and traditional pest control measures (Flint and
Van den Bosch 1981). BCAs have the potential to be self-sustainable. They display
host specificity, non-phytotoxicity and human safety. However, a pre-condition for
successful use of BCAs is the basic knowledge of ecology of both pest and natural
enemy. Under a set circumstance, sometimes, the use of BCAs can prove to be more
cost-effective than the conventional chemical control measures. On the other hand,
BCAs also have some serious disadvantages because of which they are not able to
cope with the competitive markets of the less expensive and more effective
chemical pesticides. Most BCAs are niche products. And unlike the synthetic
insecticides, these do not show immediate effects on the pest upon application.
Additionally, the stringent and expensive approval and registration processes in
case of the microbial BCAs significantly increase their cost of production (Mallet
1989). Lack of environmental persistence and low, unpredictable efficacy under
field conditions are a few other limitations for the success of BCAs.
In the present chapter, an overview of the soil microorganisms including viruses,
bacteria, fungi and nematodes pathogenic to lepidopteran insect pests and their use
as effective BCAs have been outlined.
1.3 Entomopathogenic Soil Microorganisms
The entomopathogens have a wide distribution in the natural environment and are
reported to cause diseases in many insect pest species, thereby emerging as poten-
tial BCAs. As many as 3,000 entomopathogens have been reported. A number of
these entomopathogenic microorganisms can be mass produced, formulated appro-
priately and applied in the pest-infested crop fields with ease. The different cate-
gories of entomopathogens are detailed as below.
1.3.1 Entomopathogenic Viruses
Although many families of viruses have been shown to be infective to insects and
invertebrates in general (Fauquet et al. 2005), much of the research on use and
development of virus-based bioinsecticides has been concentrated towards
baculoviruses. This is based on the fact that baculoviruses act as potent natural
enemies of insect pests, lepidopteran pests to be more specific (Caballero
et al. 1992). Although not very related, it is worth a mention that much of the
advancements in the field of baculoviruses is also due to their use as eukaryotic
expression vector systems. They have rod-shaped enveloped virions with single,
covalently closed circular double-stranded DNA as their genome (Theilmann
et al. 2005). Occlusion-derived virions (ODVs) and the budded virions (BVs)
represent the two typical phenotypes of virions which occur in baculovirus
infections. The ODVs are occluded, towards the end of the virus replication in the
infected cell nucleus, in a crystalline protein matrix. Horizontal transmission of
baculoviruses occurs through ODVs. They initiate virus infection in the insect
midgut upon ingestion of the virus occlusion body (OB). BVs are produced when
nucleocapsids bud through the plasma membrane of virus-infected cells. These are
responsible for causing a systemic infection by spreading the virus to tissues
throughout the host (Erlandson and Theilmann 2009).
The baculovirus-based bioinsecticides are host specific and safe to environment,
human and animal health with no toxic residues and can be combined with other
strategies for IPM (Monobrullah 2003; Ashour et al. 2007). Detailed classification,
morphology and use of baculoviruses for control of lepidopteran pests are given
under Chap. 2 of this volume by Ranga Rao et al.
1.3.2 Entomopathogenic Bacteria
Over a century back, a moth infected with a bacterium was found in Thuringia,
Germany, by Ernst Berliner (Siegel 2000). This bacterium was isolated, identified
and named as Bacillus thuringiensis. In the past few decades, research on
B. thuringiensis has exploded into diverse innovative directions for its use as
BCA and has also triggered research on other potential entomopathogenic bacteria.
Entomopathogenic bacteria fall into two categories, obligate and facultative
pathogens (Bucher 1960; Krieg 1981). Obligate entomopathogenic bacteria are
capable of damaging a healthy intact midgut wall of the insect larvae. A gram-
positive spore-forming bacterium, B. thuringiensis, belongs to this class (Schnepf
et al. 1998). In contrast, the facultative entomopathogens like Serratia marcescens
can disrupt the gut wall only when the insect is under some kind of stress
(Matsumoto et al. 1998) and need to be injected directly into the haemocoel for
the bacterium to cause septicaemia.
The entomopathogenic bacteria have developed an array of strategies to infect
and kill the host larvae. These pathogens make use of diverse virulence factors such
as insecticidal toxins, exoenzymes and haemolysins. B. thuringiensis produces
different kinds of toxin proteins at different phases which show potent insecticidal
activities. Cry and Cyt toxins are produced as parasporal crystals during the
sporulation phase. After ingestion, the Cry toxin binds to specific receptors in the
insect midgut epithelium. By the action of proteases, the protoxin is cleaved
releasing the active toxin. The activated toxin induces pore formation in the
epithelial cells of the larval midgut leading to their lysis and subsequent kill of
the insect larva (Daniel et al. 2000). Over 400 genes coding for these toxin variants
have been identified from different strains of B. thuringiensis (Crickmore
et al. 2007). The insecticidal spectrum of each of these toxin variants is distinct.
Vegetative insecticidal proteins (Vip) are a set of toxins produced both during the
vegetative and sporulation phases of the bacterium. Upon ingestion, Vip toxins
cause swelling and osmotic lysis of epithelial cells of the midgut leading to host
death (Estruch et al. 1996; Crickmore et al. 2007). A detailed view on this topic is
given in Chaps. 3 and 4 of this volume.
Making the plants resistant to insect pests through transgenic approach by
transforming them with cry genes (Vaeck et al. 1987; Romeis et al. 2006) has
attained a great success. One of the examples which gained commercial success is
‘Bt Cotton’. However, development of resistance by the insect pests to these toxins
is raising concerns in this area of research. One such case has been reported recently
where it was shown that the cry1Ac toxin had a reduced binding capacity to the
receptors on the brush border membrane vesicles of the field-collected cry1Ac-
resistant pink bollworms (Ojha et al. 2014). Further, the safety and public accep-
tance of the genetically modified foods is also raising concerns for the commercia-
lisation of transgenic crops (Shelton et al. 2002).
Research is also being focused on investigating other entomopathogenic bacteria
like the species of Photorhabdus and Xenorhabdus. They are gram-negative motile
bacteria living as obligate mutualists in association with the nematodes,
Heterorhabditis and Steinernema, respectively (Waterfield et al. 2009; Castagnola
and Stock 2014). The parasitic nematodes infect the insect pests and carry along the
symbiotic bacteria from one larval host to another. These bacteria have been shown
to have insecticidal properties (Silva et al. 2002; Waterfield et al. 2009) and have
also displayed a plethora of toxin proteins, viz., toxin complex (Tc) proteins
(Bowen and Ensign 1998), Photorhabdus insect-related (Pir) proteins (Waterfield
et al. 2005) and insecticidal pilin proteins (Khandelwal et al. 2004). More infor-
mation on nematode-associated entomopathogenic bacteria and a detailed review
on their toxins can be referred from Chaps. 5 and 13 of this volume.
1.3.3 Entomopathogenic Fungi
Entomopathogenic fungi, for decades now, provide a feasible system for insect pest
control and have served as model for plant-pathogen interaction studies. This group
of entomopathogens are the most commonly reported natural enemies in the
agricultural fields causing regular epizootics (Rios-Velasco et al. 2010). Consider-
ing entomopathogenic fungi as a whole, they infect a wide range of insect species
including lepidopterans, but the individual species and strains of entomopathogenic
fungus have specific host range and virulence. About 750 fungal species have been
reported to be entomopathogenic although a very small number of them are used as
BCAs. The species belonging to the genera Metarhizium (Pandey and Hasan 2009),
Beauveria (Garcia et al. 2011), Nomuraea (Ingle et al. 2004), Lecanicillium (Anand
et al. 2009) and Isaria (Zimmermann 2008) have been well worked out with
noteworthy contributions.
These fungi naturally inhabit the soils worldwide and can be isolated from both
soil- and fungus-infected insect cadavers. Fungal conidia are the infective propa-
gules which when in contact with a susceptible host attach to its cuticle and develop
a germ tube. This is however accompanied by an array of molecular responses in
both the host and the pathogen. The formation of appressoria, penetration peg and
the secretion of hydrolytic enzymes help the fungus to enter the insect’s body. Once
inside, the fungal mycelium takes up nutrients from the host for its own nourish-
ment and causes mechanical injury to the insect leading to its death. The hyphae
pierce out of the insect cadaver and sporulate on its surface. The dispersal of these
conidia aids in spreading the disease and in continuing the fungal life cycle on a
new host (Shah and Pell 2003; Schrank and Vainstein 2010). The advantage that the
fungal BCAs have over others is that they can infect any developmental stage of the
insect (Anand et al. 2009; Anand and Tiwary 2009) and are environmentally safe,
also to humans (Goettel et al. 2001; Kubicek and Druzhinina 2007). More details on
entomopathogenic fungi can be referred to in Chaps. 6, 7 and 10–12 of this volume.
Apart from the mechanical injury to the insect host, the fungus also produces
toxins which are insecticidal in nature (Sree et al. 2008). These mycotoxins are
non-ribosomal peptides and can be classified into chain peptides or cyclic peptides
based on their chemical structure. A number of toxins have been reported from
different entomopathogenic fungi, some of them include destruxins (Pedras
et al. 2002; Sree and Padmaja 2008; Liu and Tzeng 2012), bassianolides (Suzuki
et al. 1977), beauvericins (Wang and Xu 2012), enniatins (Mule et al. 1992; Hiraga
et al. 2005), efrapeptins (Lardy et al. 1975) and so on. Chapters 8–11 of this volume
provide more details on these mycotoxins.
1.3.4 Entomopathogenic Nematodes
Entomopathogenic nematodes are obligate parasites of insect species and are

associated with symbiotic bacteria which also show potent insecticidal properties
(Waterfield et al. 2009; Stock and Goodrich-Blair 2012). These nematodes belong
to either of the two families, Steinernematidae and Heterorhabditidae. Their poten-
tial as insecticidal agents has been investigated against a wide range of insect
species including lepidopteran pests by many researchers around the world (Begley
1990; Grewal et al. 2005; Hussain and Ahmad 2011; Lacey and Georgis 2012). The
juvenile nematodes parasitise their host insects by directly penetrating the cuticle or
by entry through the natural openings. The symbiotic bacteria associated with the
nematodes are regurgitated in the host, which then multiply rapidly and cause host
death by septicaemia, often within 48 h. The bacterial attack causes break down of
the host tissue, thereby providing nourishment to the nematodes. Subsequent to host
insect death, the juvenile nematodes develop into adults and reproduce, raising its
next generation (Nguyen and Smart 1990; Jackson and Brooks 1995).
Use of entomopathogenic nematodes as BCAs has undergone a lot of develop-
ment over the past few decades (Shapiro-IIan et al. 2002). Foliar (Arthurs
et al. 2004) and epigeal applications of these nematodes have been investigated
(Shapiro-Ilan et al. 2006). Epigeal or soil surface application has yielded better
results than foliar application owing to the effect of abiotic stresses on the
nematodes sprayed on the foliage (Begley 1990). More elaborate details on

entomopathogenic nematodes are available in Chaps. 13–15 of this volume.
1.4 Conclusions and Future Prospects
The insect order Lepidoptera comprises of a large number of economically impor-

tant pests. The control of these pests in an environmentally sustainable manner is
very important in the current scenario of environment protection. The use of BCAs
like viruses, bacteria, fungi and nematodes plays a major role in this perspective.
Also, the secondary metabolites from these microorganisms are being exploited for
the development of eco-friendly bioinsecticides. Research is progressing towards
the efficient use of these microorganisms and their metabolites in IPM. The rest of
the 15 book chapters of the present book volume describe in detail the use of
these BCAs.
Acknowledgements KSS is thankful to the SERB, Govt. of India, for financial assistance through
the Fast Track Young Scientist scheme.
References
Anand R, Tiwary BN (2009) Pathogenicity of entomopathogenic fungi to eggs and larvae of

Spodoptera litura, the common cutworm. Biocontrol Sci Technol 19:919–929
Anand R, Prasad B, Tiwary BN (2009) Relative susceptibility of Spodoptera litura pupae to
selected entomopathogenic fungi. Biocontrol 54:85–92
Arthurs S, Heinz KM, Prasifka JR (2004) An analysis of using entomopathogenic nematodes
against above-ground pests. Bull Entomol Res 94:297–306
Ashour MB, Ragheb DA, EI-Sheikh EI-SA, Gomaa EI-AA, Kamita SG, Hammock BD (2007)
Biosafety of recombinant and wild type nucleopolyhedrovirus as bioinsecticides. Int. J.
Environ Res Public Health 4:111–125
Bale JS, van Lenteren JC, Bigler F (2008) Biological control and sustainable food production.
Philos Trans R Soc B 363:761–776
Begley JW (1990) Efficacy against insects in habitats other than soil. In: Gaugler R, Kaya HK
(eds) Entomopathogenic nematodes in biological control. CRC, Boca Raton, pp 215–231
Benton TG, Vickery JA, Wilson JD (2003) Farmland biodiversity: is habitat heterogeneity the
key? Trends Ecol Evol 18:182–188
Bianchi FJJA, Booij CJH, Tscharntke T (2006) Sustainable pest regulation in agricultural land-
scapes: a review on landscape composition, biodiversity and natural pest control. Proc Biol Sci
273:1715–1727
Bowen DJ, Ensign JC (1998) Purification and characterization of a high-molecular-weight insec-
ticidal protein complex produced by the entomopathogenic bacterium Photorhabdus
luminescens. Appl Environ Microbiol 64:3029–3035
Bucher GE (1960) Potential bacterial pathogens of insects and their characteristics. J Insect Pathol
2:172–195
Caballero P, Aldebis HK, Vargas-Osuna E, Santiago-Alvarez C (1992) Epizootics caused by a

nuclear polyhedrosis virus (NPV) in populations of Spodoptera exigua in southern Spain.
Biocontrol Sci Technol 2:45–149
Carson R (1962) Silent spring. Houghton Mifflin Publications, Boston, pp 15–39
Castagnola A, Stock P (2014) Common virulence factors and tissue targets of entomopathogenic
bacteria for biological control of lepidopteran pests. Insects 5:139–166
Collier R, Fellows J, Adams S, Semenov M, Thomas B (2008) Vulnerability of horticultural crop
production to extreme weather events. In: Halford N, Jones HD, Lawlor D (eds) Effects of
climate change on plants: implications for agriculture. Association of Applied Biologists,
Warwick, pp 3–13
Crickmore N, Zeigler DR, Schnepf E, Van Rie J et al (2007) Bacillus thuringiensis toxin
nomenclature. http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/
Daniel JL, David JE, Paul J (2000) Role of proteolysis in determining potency of Bacillus
thuringiensis Cry1Ac-endotoxin. Appl Environ Microbiol 66:5174–5181
Easterling D, Meehl GA, Parmesan C, Changnon SA, Karl TR, Mearns LO (2000) Climate
extremes: observations, modeling and impacts. Science 289:2068–2074
Erlandson MA, Theilmann DA (2009) Molecular approaches to virus characterisation and detec-
tion. In: Stock SP et al (eds) Insect pathogens: molecular approaches and techniques. CAB
International, Wallingford, pp 3–31
Estruch JJ, Waren GW, Mullis MA, Nye GJ et al (1996) Vip 3A, a novel Bacillus thuringiensis
vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects.
Proc Natl Acad Sci U S A 93:5389–5394
Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) (2005) Virus taxonomy,
Eighth Report of the International Committee on taxonomy of viruses. Elsevier/Academic,
London
Fenster CB, Armbruster WS, Wilson P, Thomson JD, Dudash MR (2004) Pollination syndromes
and floral specialization. Annu Rev Ecol Evol Syst 35:375–403
Flint ML, Van den Bosch R (1981) Introduction to integrated pest management. Plenum,
New York
Garcia GC, Berenice GMM, Nestor BM (2011) Pathogenicity of isolates of entomopathogenic
fungi against Spodoptera frugiperda (Lepidoptera: Noctuidae) and Epilachna varivestis (Cole-
optera: Coccinellidae). Rev Colomb Entomol 37:217–222
Goettel MS, Hajek AE, Siegel JP, Evans HC (2001) Safety of fungal biocontrol agents. In: Butt
TM, Jackson CW, Magan N (eds) Fungi as biocontrol agents: progress, problems and potential.
CABI Publishing, Wallingford, pp 347–376
Grewal PS, Ehlers R-U, Shapiro-Ilan DI (eds) (2005) Nematodes as biocontrol agents. CABI
Publishing, Wallingford, p 505
Gurr GM, Wratten SD, Luna JM (2003) Multi-function agricultural biodiversity: pest management
and other benefits. Basic Appl Ecol 4:107–116
Hajek A (2004) Natural enemies: an introduction to biological control. Cambridge University
Press, Cambridge
Hiraga K, Yamamoto S, Fukuda H, Hamanaka N, Oda K (2005) Enniatin has a new function as an
inhibitor of Pdr5p, one of the ABC transporters in Saccharomyces cerevisiae. Biochem
Biophys Res Commun 328:1119–1125
Hussain MA, Ahmad W (2011) Management of Helicoverpa armigera by entomopathogenic
nematodes. Lambert Academic Publishing GmbH & Co. KG, Germany, p 168
Ingle YV, Lande SK, Burgoni GK, Autkar SS (2004) Natural epizootic of Nomuraea rileyi on
lepidopterous pests of soybean and green gram. J Appl Zoolog Res 15:160–162
Ives AR, Klug JL, Gross K (2000) Stability and species richness in complex communities. Ecol
Lett 3:399–411
Jackson JJ, Brooks MA (1995) Parasitism of western corn rootworm larvae and pupae by
Steinernema carpocapsae. J Nematol 27:15–20
Kevan PG, Baker HG (1983) Insects as flower visitors and pollinators. Annu Rev Entomol 28:407–
453
Khandelwal P, Choudhury D, Birah A, Reddy MK, Gupta GP, Banerjee N (2004) Insecticidal pilin
subunit from the insect pathogen Xenorhabdus nematophila. J Bacteriol 186:6465–6476
Klaassen CD, Amdur MO, Doull J (eds) (1996) Casarett and Doull’s toxicology. The basic science
of poisons, 5th edn. McGraw-Hill Companies, Toronto
Krenn HW (2010) Feeding mechanisms of adult Lepidoptera: structure, function, and evolution of
the mouthparts. Annu Rev Entomol 55:307–327
Krieg A (1981) The genus Bacillus: insect pathogens. In: Starr MP, Stolp H, Trueper HG,
Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin, pp 1743–1755
Kubicek CP, Druzhinina IS (2007) Environmental and microbial relationships, 2nd edn, The
Mycota IV. Springer, Berlin, pp 159–187
Lacey LA, Georgis R (2012) Entomopathogenic nematodes for control of insect pests above and
below ground with comments on commercial production. J Nematol 44:218–225
Lardy H, Reed P, Lin C-HC (1975) Antibiotic inhibitors of mitochondrial ATP synthesis. Fed Proc
Fed Am Soc Exp Biol 34:1707–1710
Liu BL, Tzeng YM (2012) Development and applications of destruxins: a review. Biotechnol Adv
30:1242–1254
Lonsdale D, Gibbs JN (2002) Effects of climate change on fungal diseases of trees. In:
Broadmeadow MSJ (ed) Climate change: impacts on UK forests, Forestry Commission
Bulletin No 125. Forestry Commission, Edinburgh, pp 83–97
Mallet J (1989) The evolution of insecticide resistance: have the insect won? Trends Ecol Evol
4:336–340
Matsumoto H, Noguchi H, Hayakawa Y (1998) Primary cause of mortality in the armyworm
larvae simultaneously parasitized by parasitic wasp and infected with bacteria. Eur J Biochem
252:299–304
Meiners RE, Morriss AP (2001) The legacy of the DDT Ban, DDT’s legacy: malaria’s return.
Property and Environment Research Center Reports 19
Monobrullah M (2003) Optical brighteners - pathogenecity enhancers of entomopathogenic
viruses. Curr Sci 84:640–645
Mule G, D’Ambrosio A, Logrieco A, Bottalico A (1992) Toxicity of mycotoxins of Fusarium
sambucinum for feeding in Galleria mellonella. Entomol Exp Appl 62:17–22
Nguyen KB, Smart GC (1990) Steinernema scapterisci n. sp. (Steinernematidae: Nematoda). J
Nematol 22:187–199
Oerke EC, Dehne HW, Schnbeck F, Weber A (1994) Crop production and crop protection:
estimated losses in major food and cash crops. Elsevier, Amsterdam
Ojha A, Sree KS, Sachdev B, Rashmi MA, Ravi KC, Suresh PJ, Mohan KS, Bhatnagar RK (2014)
Analysis of resistance to Cry1Ac in field-collected pink bollworm, Pectinophora gossypiella
(Lepidoptera: Gelechiidae), populations. GM Crops Food 5:280–286. doi:10.4161/21645698.
2014.947800
Pandey R, Hasan W (2009) Pathogenicity of entomopathogenic fungi, Metarhizium anisopliae
against tobacco caterpillar, Spodoptera litura (Fabricius). Trends Biosci 2:29–30
Pedras MSC, Irina ZL, Ward DE (2002) The destruxins: synthesis, biosynthesis, biotransformation
and biological activity. Phytochemistry 59:579–596
Rios-Velasco C, Cerna-Chavez E, Pena SS, Morales GG (2010) Natural epizootic of the
entomopathogenic fungus Nomuraea rileyi (Farlow) Samson infecting Spodoptera frugiperda
(Lepidoptera: Noctuidae) in Coahuila Mexico. J Res Lepid 43:7–8
Robinson RA, Sutherland WJ (2002) Post-war changes in arable farming and biodiversity in Great
Britain. J Appl Ecol 39:157–176
Romeis J, Meissle M, Bigler F (2006) Transgenic crops expressing Bacillus thuringiensis toxins
and biological control. Nat Biotech 24:63–71
Schnepf HE, Cricmore N, Vanrie J, Lereclus D, Baum J, Feitelson J, Zfider DR, Dean DH (1998)
Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62:775–806
Schrank A, Vainstein MH (2010) Metarhizium anisopliae enzymes and toxins. Toxicon 56:1267–
1274
Shah PA, Pell JK (2003) Entomopathogenic fungi as biological control agents. Appl Microbiol
Biotechnol 61:413–423
Shapiro-IIan DI, Gouge DH, Koppenhöfer AM (2002) Factors affecting commercial success: case
studies in cotton, turf and citrus. In: Gaugler R (ed) Entomopathogenic nematology. CABI
Publishing, Wallingford, pp 333–356
Shapiro-Ilan DI, Gouge DH, Piggott SJ, Fife JP (2006) Application technology and environmental
considerations for use of entomopathogenic nematodes in biological control. Biol Control
38:124–133
Shelton AM, Zhao J-Z, Roush RT (2002) Economic, ecological, food safety and social conse-
quences of the deployment of Bt transgenic plants. Annu Rev Entomol 47:845–881
Siegel JP (2000) Bacteria. In: Lacey LL, Kaya HK (eds) Field manual of techniques in invertebrate
pathology. Kluwer Academic, Boston, pp 209–230
Silva CP, Waterfield NR, Daborn PJ, Dean P, Chilver T, Au CP, Sharma S, Potter U, Reynolds SE,
ffrench-Constant R (2002) Bacterial infection of a model insect: Photorhabdus luminescens
and Manduca sexta. Cell Microbiol 4:329–339
Sree KS, Padmaja V (2008) Oxidative stress induced by destruxin from Metarhizium anisopliae
(Metch.) involves changes in glutathione and ascorbate metabolism and instigates ultrastruc-
tural changes in the salivary glands of Spodoptera litura (Fab.) larvae. Toxicon 51:1140–1150
Sree KS, Padmaja V, Murthy LNY (2008) Insecticidal activity of destruxin, a mycotoxin from
Metarhizium anisopliae (Hypocreales), against Spodoptera litura (Lepidoptera: Noctuidae)
larval stages. Pest Manag Sci 64:119–125
Stock SP, Goodrich-Blair H (2012) Nematode parasites, pathogens and associates of insects and
invertebrates of economic importance. In: Lacey LA (ed) Manual of techniques in invertebrate
pathology, 2nd edn. Academic, San Diego, pp 373–426
Suzuki A, Kanaoka M, Isogai A, Murakoshi S, Ichinoe M, Tamura S (1977) Bassianolide, a new
insecticidal cyclodepsipeptide from Beauveria bassiana and Verticillium lecanii. Tetrahedron
Lett 18:2167–2170
Theilmann DA, Blissard GW, Bonning B, Jehle J, O’Reilly DR, Rohrmann GF, Theim S, Vlak J
(2005) Family Baculoviridae. In: Fauquet CM, Mayo MA, Maniloff M, Desselberger U, Ball
LA (eds) Virus taxonomy. Eighth report of the International committee on virus taxonomy.
Elsevier, San Diego, pp 177–185
Turner JA (2008) Tracking changes in the importance and distribution of diseases under climate
change. In: Proceedings HGCA R&D conference: arable cropping in a changing climate 2008,
pp 68–77
Vaeck M, Reynaerts A, Hofte H, Jansens S et al (1987) Transgenic plants protected from insect
attack. Nature 328:33–37
Wang Q, Xu L (2012) Beauvericin, a bioactive compound produced by fungi: a short review.
Molecules 17:2367–2377
Waterfield N, Kamita SG, Hammock BD, ffrench-Constant R (2005) The Photorhabdus Pir toxins
are similar to a developmentally regulated insect protein but show no juvenile hormone
esterase activity. FEMS Microbiol Lett 245:47–52
Waterfield NR, Ciche T, Clarke D (2009) Photorhabdus and a host of hosts. Annu Rev Microbiol
63:557–574
Wilby A, Thomas MB (2002) Natural enemy diversity and pest control: patterns of pest emergence
with agricultural intensification. Ecol Lett 5:353–360
Zimmermann G (2008) The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces
farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces
fumosoroseus): biology, ecology and use in biological control. Biocontrol Sci Technol
18:865–901
Chapter 2
Role of Nucleopolyhedroviruses (NPVs)
in the Management of Lepidopteran Pests
in Asia
G.V. Ranga Rao, Ch. Sridhar Kumar, K. Sireesha, and P. Lava Kumar
2.1 Introduction
The use of synthetic insecticides has been the major approach in modern agriculture
for controlling insect pests on different crops in most of the developing countries.
Chemical control is one of the effective and quicker methods in reducing pest
population, where farmer obtains spectacular results within a short period. How-
ever, overreliance and indiscriminate unscientific use of pesticides for longer
periods resulted in a series of problems, mainly risk of environmental contamina-
tion, loss of biodiversity, development of insecticide-resistant pest populations,
resurgence, outbreaks of the secondary pests, increase in inputs on chemicals and
toxicological hazards due to accumulation of pesticide residues in the food chain,
etc., ultimately contributing not only to inefficient insect control but also environ-
mental and health hazards (Armes et al. 1992; Kranthi et al. 2002). Therefore, there
is an urgent need to rationalize the use of chemical pesticides for the management
of insect pests. In recent years, the growing public concern over potential health
hazards of synthetic pesticides has led to the exploration of alternative pest man-
agement options, such as adoption of integrated pest management (IPM). IPM
combines cultural, biological, and chemical measures in the most effective, envi-
ronmentally sound, and socially acceptable way of managing pests, diseases, and
weeds. IPM aims at suppressing the pest population by combining available
methods in a harmonious way with emphasis on farm health and net returns. In
an attempt to overcome the present crisis and to find alternatives to synthetic
G.V.R. Rao (*) • Ch.S. Kumar • K. Sireesha

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru,
Telangana 502324, India
P.L. Kumar
International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, PMB 5320, Nigeria

DOI 10.1007/978-3-319-14499-3_2
12 G.V.R. Rao et al.
insecticides, the application of “bio-pesticides” as an eco-friendly measure for pest

suppression has come up as one of the effective tools in IPM approach.
Bio-pesticides are developed from natural plant or animal origin, which can
intervene in the life cycle of insect pests in such a way that the crop damage is
minimized. The biological agents employed for this purpose include parasites,
predators, and disease-causing fungi, bacteria, nematodes, and viruses, which are
the natural enemies/pathogens of pests. More than three thousand microorganisms,
comprising viruses, bacteria, fungi, protozoa, and nematodes, have been reported as
insect pathogens. Of these, microbial pathogens gained significance for use as
bio-pesticides primarily due to ease in production, application, wider adoptability,
persistence, economic feasibility, and environmental compatibility. Many species
of insect pathogenic microorganisms have been exploited as bio-pesticides, and
some species have been developed into commercial formulations that are being
used in many countries. Though farmers in Asia are aware of the importance of IPM
and its impact on health and environment, the adoption level was not up to the
expected levels. However, recent estimates are quite encouraging with reduction in
chemical use to $25.3 billion in 2010 compared to $26.7 billion in 2005. On the
other hand, interestingly, the bio-pesticides market is growing rapidly from $672
million in 2005 to over $1 billion in 2010 (Anon 2009). Several viruses belonging to
18 different families are known to infect invertebrates and insects (Fauquet
et al. 2004). However, bio-pesticide development is concerned almost exclusively
with members of one family, the Baculoviridae, because of their common occur-
rence in most important insect pests primarily in the order of Lepidoptera and their
action as natural regulators of pest populations (Weiser 1987; Gelernter and
Federici 1990; Caballero et al. 1992; Blissard et al. 2000). The potential of
baculoviruses to be employed as insecticides is known since more than 75 years
ago (Benz 1986). To date, over 30 different baculoviruses are used to control
several insect pests in agriculture and forestry (Moscardi 1999). The use of
baculovirus as insecticides is based on a set of useful properties, such as pathoge-
nicity, specificity, narrow host range, environmental persistence, suitability to add
to other bio-agents with synergism, and ability to induce epizootics. There are
several advantages of using insect viruses in pest management over traditional
synthetic chemical insecticides: these are highly host specific and are known to
be completely safe to humans, animals, and non-target beneficial insects such as
bees, predatory insects, and parasitoids (Groner 1986; Monobrullah and Nagata
1999; Nakai et al. 2003; Ashour et al. 2007); lack of toxic residues allowing
growers to treat their crops even shortly before harvest, with low probability to
develop stable resistance (Monobrullah 2003). These are highly compatible with
other methods of pest control and are well suited for use in IPM programs. Another
important reason for the interest in baculoviruses as potential insect control agents
is that they are relatively easy to visualize and monitor using a light microscope. In
vivo and in vitro tests with several vertebrate, invertebrate, and plant species have
not demonstrated any pathogenic, toxic, carcinogenic, or teratogenetic effects after
exposure to these viruses (Ignoffo and Rafajko 1972; Ignoffo 1973; Banowetz
et al. 1976; Lautenschlager et al. 1977; Roder and Punter 1977; Huber and Krieg
2 Role of Nucleopolyhedroviruses (NPVs) in the Management of Lepidopteran. . . 13
1978). Baculoviruses are stable and can be stored as aqueous suspensions or dried
powders for long periods without any loss of activity (David and Gardiner 1967a).
They are resistant to many chemicals and persist in the soil for many years (David
and Gardiner 1967b), and their activity is not altered significantly by relative
humidity (David et al. 1971), precipitation (David and Gardiner 1966), or
prolonged exposure to normal field temperatures (Yendol and Hamlen 1973).
They can be used concurrently with most chemical insecticides and permit the
reduction of the number of applications needed to keep the insect plague under
control in crops, thus contributing to the reduction of the costs of protection.
Finally, its use in replacement of synthetic insecticides helps to reduce the overall
levels of chemical pollution (Falcon 1971; Hunter et al. 1975; Jacques and Long
1978). Baculoviruses differ significantly from chemical insecticides in that they are
components of nature. Large quantities of virus are released into the environment
during natural epizootics, which are common, widespread, and often important in
regulating insect population levels (Injac 1973; Federici 1978). There is evidence
that the amount of virus which is artificially placed into the environment as
bio-pesticide is minimal compared with the amount produced during such epizo-
otics (Thomas 1975).
2.2 Classification of Baculoviruses
Baculoviruses are occluded, double-stranded DNA (dsDNA) viruses and charac-

terized by the presence of occlusion or inclusion bodies (OBs). The nature and
significance of these OBs remained a mystery for a long time until the electron
microscope (EM) was available that the virus particle could be isolated and
identified as the infectious viral agent. Based on the size, shape, and occluded
virion phenotype, the baculoviruses are classified into two genera, nucleopolyhe-
droviruses (NPVs) and granuloviruses (GVs) (Rohrmann 1999; Winstanley and
O’Reilly 1999; Blissard et al. 2000; Fauquet et al. 2004). The EM observation of
NPVs reveals polyhedral to irregular shaped OBs with size 0.15–15 μm in diameter
(Figs. 2.1 and 2.2) composed of matrix protein (30–40 % of total viral protein)
called polyhedrin, which crystallizes around many enveloped nucleocapsids (Hooft
van Iddekinge et al. 1983). Different NPVs are characterized by their occluded
virions being present either as single (SNPV) or multiple (MNPV) nucleocapsids
within the envelope (Figs. 2.1 and 2.3). Both SNPVs and MNPVs may contain 20–
200 virions depending upon species (Rohrmann 1999). The GVs have small OBs
(0.25–0.5 μm in cross section), are ellipsoidal in shape, and normally contain a
single nucleocapsid, which is enveloped and is composed of a major matrix protein
called granulin (Funk et al. 1997; Winstanley and O’Reilly 1999). NPVs are found
mostly in the order Lepidoptera, Hymenoptera (31 species), Diptera (27 species),
and Coleoptera (5 species) as well as from the crustacean class Decapoda (shrimp),
whereas GVs are only found within the order Lepidoptera (Federici 1997; Blissard
et al. 2000). Virions consist of one or more nucleocapsids embedded in a
Fig. 2.1 Morphological characteristics of nucleopolyhedroviruses (NPVs) and granuloviruses

(GVs)
Fig. 2.2 Scanning electron micrograph of POBs: scanning electron micrograph (SEM) images of
occlusion bodies (OBs) of baculoviruses. The purified aqueous OBs of baculoviruses isolated from
(a) H. armigera, (b) S. litura, and (c) A. albistriga were dehydrated, mounted over the stubs,
applied with a thin layer of gold metal over the sample using sputter coater, and then scanned
under EM. Magnification ¼ 6,500
membranous envelope. Two morphologically distinct but genetically identical viral

forms are produced during postinfection: (a) budded virus particles (BV) which
serve for the transmission of the virus to other tissues of the infected pest and
Fig. 2.3 Transmission electron micrographs of the cross section of poly-occlusion bodies (POBs):
Pellets of purified OBs of baculoviruses isolated from (a) H. armigera, (b) S. litura, and (c)
A. albistriga were subjected to ultrathin sections, mounted on copper grids, and stained with
saturated aqueous uranyl acetate and counterstained with 4 % lead citrate and observed under
TEM. Magnification ¼ 25,000
(b) OBs which are responsible for the survival of the virus and spread of the disease.
The OB (polyhedra) of NPV contains many occlusion-derived virions (ODV)
surrounded by a matrix composed of mainly polyhedrin, a major structural protein
(Braunagel et al. 2003). Polyhedrin is produced in large quantities (around 30 % of
total protein mass at the time of host death). Polyhedra are relatively stable and
under favorable conditions virions can survive in the environment for more than
20 years. Under magnification of around 1,000 , polyhedra resemble clear, irreg-
ular crystals of salt; so they are big enough to be seen in a light microscope. Some
common symptoms of the virus attack are sluggishness, discoloration of skin, wet
or extremely moist droppings, and regurgitation of fluids (a sign of stress).
The size and shape of occlusion bodies in NPVs varies considerably not only
between the polyhedral occlusion bodies (POBs) from different insects but often
also within the same species. For example, majority of the POBs of Helicoverpa
armigera NPV are spherical, while some of them are irregular in shape and the size
ranges from 0.6 to 2.3 μm, averaging to 1.35 μm (Fig. 2.2). The diameter of
polyhedra ranges about 0.5–1.5 μm, depending on the insect species (Fig. 2.3).
The differentiation in the cross section of typical baculovirus (NPV and GV)
occlusion bodies (OBs) is clearly represented in Fig. 2.1. In the boundary of OB,
the protein envelope (PE) appears as an electron-dense layer made up of PE protein
or envelope protein and shown to be very sensitive to alkaline proteases (Russell
and Rohrmann 1990; Van Lent et al. 1990). The distance between envelope and
crystalline matrix (polyhedrin or granulin) is not uniform around the occlusion
body. The fine structure of occlusion body reveals crystalline lattice of the occlu-
sion body protein molecules, which are arranged in cubic system. Although there is
no true membrane covering the OB, difficulties in staining OB, the retention of their
shape, and the presence of a membrane-like coat following chemical and physical
treatment indicate that the exterior portion of OB is different from the interior
portion. On the whole, they are very stable and can persist indefinitely in the
environment (Bergold 1982). The infectious, rod-shaped virions are randomly

occluded in OBs without any apparent disruption of the lattice; an 8-nm layer
separates virion from the protein matrix. The size of the virions is with dimensions
in the range from 4.0 to 140 250 to 400 nm. Alkaline-liberated virions readily
lose their envelopes to reveal nucleocapsids each made up of a capsid surrounding a
DNA core. The capsid, in turn, consists of protein subunits arranged along its long
axis. The virions contain large circular, covalently closed, dsDNA genome with
size in range of 80–180 kbp packed in the nucleocapsid (Blissard and Rohrmann
1990; Volkman et al. 1995).
2.3 Examples of Some Commercial Baculovirus-Based

Products Registered in Different Countries for Pest
Management
Over 20 species of baculoviruses have been developed or registered as commer-

cially available insecticides, and over 30 different products have been registered as
commercial insecticides based upon NPV or GV. Autographa californica and
Anagrapha falcifera NPVs were registered in various countries and have relatively
broad host spectrum and potentially can be used on a variety of crops infested with
pests including Spodoptera and Helicoverpa. GV is the active component of a
number of bio-pesticides used for protection of apple and pear orchards against the
codling moth, Cydia pomonella. Some of the trademarks of GV-based products are
the following: Granusal™ in Germany, Carpovirusine™ in France, Madex™ and
Granupom™ in Switzerland, and Virin-CyAP in Russia. Annually, up to
250,000 ha of orchards have been protected with Madex™ in different European
countries (Vincent et al. 2007). Another GV infecting Erinnyis ello (cassava
hornworm) was found to be very efficient in protection of cassava plantations
(Bellotti 1999). This GV has been used for spraying cassava crops in South
American countries. Two commercial preparations based on Spodoptera NPV
have been available. These are SPOD-X™ containing Spodoptera exigua NPV to
control insects on vegetable crops and Spodopterin™ containing Spodoptera
littoralis NPV which is used to protect cotton, corn, and tomatoes (Szewczyk
et al. 2006). In China, twelve baculoviruses have been authorized as commercial
insecticides (Sun and Peng 2007), including H. armigera NPV (the most widely
used virus in China for cotton, pepper, and tobacco protection), Spodoptera litura
NPV (vegetables), S. exigua NPV (vegetables), Buzura suppressaria NPV (tea),
Pieris rapae GV, and Plutella xylostella GV (vegetables). China is the largest user
of baculoviruses worldwide, with maximum number of viruses being registered for
insect control. The well-known success of employing baculovirus as a bio-pesticide
is the case of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) used to
control the velvetbean caterpillar in soybean (Moscardi 1999). This program was
implemented in Brazil in the early 1980s and came up to over 2,000,000 ha of
soybean treated annually with the virus. The use of AgMNPV in Brazil brought
about many economical, ecological, and social benefits. The protection of soybean
fields in Brazil has proven that baculoviral control agents can be effectively
produced on a large scale and they may be an alternative to broad-spectrum
chemical insecticides. The forests of temperate regions are very often attacked
and defoliated by larvae of Lepidoptera (the most common pest species are
Lymantria dispar, Lymantria monacha, Orgiya pseudotsugata, and Panolis
flammea) and some hymenopteran species (mainly Neodiprion sertifer and Diprion
pini). L. dispar MNPV formulations marketed under trade names Gypchek,
Disparivirus, and Virin-ENSH and O. pseudotsugata MNPV under trade names
BioControl-1 and Virtuss (Reardon et al. 1996) are sometimes used for forest
protection. Forest ecosystems tend to be more stable than agricultural systems,
allowing for natural or applied baculoviruses to remain in the environment for long
periods of time increasing the chance of natural epizootics.
2.4 Isolation and Characterization
Among the baculoviruses, NPVs attracted more attention of plant protection scien-
tists who were looking for an alternative to pesticides because they cause a highly
infectious disease that kills the pest within 5–7 days. These viruses attack some of
the most important Lepidopteran crop pests including the species of Helicoverpa,
Spodoptera, and Amsacta. Some of the related GV species are also highly infec-
tious, e.g., Cydia pomonella (apple codling moth) GV and P. xylostella (diamond
back moth) GV. However, not all GVs are as fast acting as NPV because morpho-
logically they had single envelope with single nucleocapsid per occlusion body
(Winstanley and O’Reilly 1999) (Fig. 2.1). In general, the host range of most NPV
is restricted to one or a few species of the genus or family of the host where they
were originally isolated. However, it also addresses an important commercial
drawback, restricting the use of these products to specific pest or closely related
pest complexes, such as Helicoverpa species (Chakraborthy et al. 1999). Some of
the few exceptions having a broader host range are (1) A. californica MNPV
infecting more than 30 species from about 10 insect families, all within the order
Lepidoptera; (2) A. falcifera NPV infecting more than 31 species of Lepidoptera
from ten insect families; and (3) Mamestra brassicae MNPV which was found to
infect 32 out of 66 tested Lepidopteran species from four different families (Groner
1986; Doyle et al. 1990; Hostetter and Puttler 1991). In contrast to NPV, the host
range of GV appears to be even narrower and mostly restricted to a single species.
In India, about 35 insect viruses have been recorded from the baculovirus group, the
most important being the NPVs of H. armigera, S. litura, Spilosoma obliqua
(Walker), Achaea janata (L.), and Amsacta albistriga (Walker) and the GVs of
A. janata, S. litura, H. armigera, and Chilo spp. (Pawar and Thombre 1992).
2.4.1 Morphological Characterization
During natural epizootic conditions, baculoviruses were isolated from larvae of

H. armigera (Hübner) (Lepidoptera: Noctuidae), S. litura (Fabricius) (Lepidoptera:
Noctuidae), and A. albistriga (Walker) (Lepidoptera: Arctiidae) at ICRISAT farms,
and the viruses isolated from these insect pests were characterized as MNPVs by
conducting morphological and biological studies (Sridhar Kumar et al. 2011). The
diseased larvae showed the typical baculovirus infection symptoms. The infected
larvae showed pale swollen bodies and are moribund. The larvae of H. armigera
and A. albistriga crawled to the top of the twigs (negative geotropism) on which
they fed (Fig. 2.4). But the larva of S. litura did not show this feature due to its soil
inhabiting nature and nocturnal habitat. The initial signs of baculoviral infection are
gradual changes in the color and luster of the integument. Infection of the epidermis
caused the host to appear soft and in some larvae the cuticle was ruptured and
discharging of body fluid onto plant parts was observed. Earlier, these symptoms
were also reported by others (Aizawa 1963; Tanada and Kaya 1993; Federici 1997).
Observation of discharged body fluid under phase contrast microscope revealed that
it consists of OBs (Fig. 2.5). Electron microscopic studies of OBs indicated that the
viruses isolated were NPVs rather than GVs. Under scanning electron microscope,
the OBs appeared as irregular shaped structures with sizes ranging from 0.5 to
2.5 μm (HaNPV), 0.9 to 2.92 μm (SlNPV), and 1.0 to 2.0 μm (AmalNPV) in
diameter (Fig. 2.2). Transmission electron microscopic (TEM) studies on cross
sections of purified POBs of these viruses showed that each occlusion body contains
2–7 (multiple) nucleocapsids packaged within a single viral envelope. The nucle-
ocapsids are elongated with parallel sides and two straight ends, measuring
277.7 41.6 nm (HaNPV), 285.7 34.2 nm (SlNPV), and 228.5 22.8 nm
(AmalNPV) (Fig. 2.3). Before characterization of any baculovirus from an insect
host, initially, it is necessary to conduct electron microscopic study (SEM and
TEM) to determine whether it is NPV or GV or SNPV or MNPV. Similarly, Tuan
et al. (1999) reported that the occlusion bodies of HaNPV and SlNPV isolated in
Taiwan were irregular shaped with sizes ranging from 0.79 0.22 μm (HaNPV)
Fig. 2.4 NPV-infected larvae of H. armigera and A. albistriga: NPV-infected larvae of

H. armigera on pigeon pea (a) and A. albistriga on groundnut (b)
Fig. 2.5 Enumeration of poly-occlusion bodies (POBs) under phase-contrast microscope: POBs
of NPVs were purified by differential centrifugation and enumerated under phase-contrast micro-
scope at 1,000 magnification
and 1.61 0.32 μm (SlNPV); both viruses were MNPVs and the nucleocapsids
were bacilliform to cylindrical tubular-shaped structures with dimensions of
319.80 7.80 44.45 4.54 nm (HaNPV) and 332.26 13.55 47.16 1.42 nm
(SlNPV). In another study, the polyhedra of the L. dispar MNPV-NM isolate were
irregularly shaped with an average diameter of 1.62 0.33 μm. TEM revealed that
LdMNPV-NM had bundles of virions in the nucleocapsid, which belonged to
MNPV (Shim et al. 2003). Wolff et al. (2002) reported the morphology of an
MNPV isolated from Lonomia obliqua (Lepidoptera: Saturniidae) with size ranging
from 1 to 1.4 μm and nucleocapsid dimensions of 270 36 nm. Ma et al. (2006)
observed the occlusion bodies in the midgut tissues of the tea looper (Ectropis
obliqua) under TEM, the micrograph showed that the EcobSNPVs were irregular
shaped with size ranging from 0.7 to 1.7 μm in diameter, and multiple rod-shaped
virions measuring about 250 40 nm were embedded in each OB with a single
nucleocapsid packaged within the envelope of the virion.
2.4.2 Biological Characterization
Biological assessment studies of the MNPVs isolated at ICRISAT farms from

H. armigera, S. litura, and A. albistriga indicated (Tables 2.1 and 2.2) that they
are highly virulent strains and have good potential for use as bio-control agents
against these important pests (Sridhar Kumar et al. 2011). The efficacy of HaNPV
isolates collected from six geographical locations of India was tested by conducting
bioassay experiments at ICRISAT with the second and third instar larvae of
H. armigera, and it was found that ICRISAT HaNPV was superior amongst the
isolates tested (Sireesha 2006). Similar differences in virulence among NPV iso-
lates have been established in previous studies conducted by Shapiro and Ignoffo
(1970) on the variations in virulence of 34 isolates of HaNPV. A 56-fold difference
in the activity of the isolates was recorded, and it was opined that the difference in
activity must be due to some characteristics of the occlusion body and/or its
occluded virions. They also did not exclude the possibility that some other factors
such as solubility of occlusions and availability of occluded virions may account for
the differences observed. Abul-Nasr and Elnagar (1980) reported the differential
biological activity of two Spodoptera littoralis isolates both at laboratory- and field-
level studies. Hughes et al. (1983) compared the time mortality response of
Heliothis zea to 14 isolates of HzNPV and identified six activity classes. Shapiro
et al. (1984) tested 19 NPV isolates of L. dispar and reported nearly 1,000-fold
difference in activity. Rabindra (1992) demonstrated the tremendous variation in
virulence among the three HaNPV isolates and recorded the lowest LC50 value of
3.467 104 POBs/ml for the HaNPV isolate from Nilgiris. Somasekhar
et al. (1993) on characterizing five Indian isolates of HaNPV found that the most
virulent isolate was that from Ooty in Tamil Nadu, India, with the lowest LC50
value of 2.538 103 POBs/ml, followed by the isolate from Coimbatore in Tamil
Nadu, India (2.965 103 POBs/ml), and the Rajasthan isolate with LC50 value of
13.08 103 POBs/ml was the least effective. Geetha and Rabindra (1999) found
that among the 11 HaNPV isolates collected from different regions in India,
Negamam and Ooty isolates from Tamil Nadu were significantly more virulent
with LC50 values of 83.807 and 93.926 POBs/cm2, respectively. The Rajasthan
isolate was the least potent with LC50 value of 111.778 POBs/cm2.
All these studies indicate that there is a significant variation in LC50 values with
overlapping fiducial limits and the use of locally produced NPV appeared to be
more useful for managing the respective insect pests than the commercially avail-
able NPV from other parts of the country. Geetha and Rabindra (1999) also reported
overlapping fiducial limits of LC50 values of eight HaNPV isolates among the
11 isolates evaluated. The variation in the activity of different isolates may be due
to different reasons. Inherent genetically controlled factors may logically be an
important reason. The other reason may be that the different isolates had different
number of passages in the host either under natural conditions or in the laboratory
(Geetha and Rabindra 1999). In Log concentration–probit mortality relationship,
the lower the slope value, the greater is the variability. Normally, the slope values
were very low in bioassay studies with insect pathogens (Burges and Thompson
1971). Battu and Ramakrishna (1987) reported the respective slope values of
0.1674, 0.4078, and 0.1215 for 6-, 9-, and 11-day-old S. obliqua larvae, respec-
tively, when inoculated with its own NPV. Arora et al. (1997) reported slope values
varying from 0.58 to 0.96 for the five HaNPV isolates evaluated against the second
instar larvae of H. armigera. The low slope of dosage–mortality curves for insect
pathogens often indicates a more stable host–pathogen relationship.
2.4.3 Comparative Analysis of Viral Proteins of Different

NPV Isolates
The crystalline matrix of the occlusion body mainly consists of a single protein,
called polyhedrin or granulin. These proteins have about 245 amino acids (29 kDa)
Table 2.1 LC50 values of NPV isolates against the second and third instar larvae on the 7th day
Regression equation Fiducial limits
NPV isolate Intercept Slope Heterogeneity LC50 (POB/ml) Lower Upper Chi-square
Second instar larvae
HaNPV 3.84 0.26 0.220 2.3 104 5.9 103 7.8 104 0.88
SlNPV 3.87 0.24 0.140 3.5 104 1.19 104 9.9 104 0.56
AmalNPV 3.87 0.23 0.060 5.6 104 2.7 104 1.15 105 0.25
Third instar larvae
HaNPV 3.66 0.26 0.09 1.5 105 6.1 104 3.3 105 0.36
SlNPV 3.14 0.34 0.2 2.4 105 8.3 104 6.1 105 0.82
AmalNPV 3.026 0.352 0.292 3.96 105 1.16 105 1.1 106 1.17
2 Role of Nucleopolyhedroviruses (NPVs) in the Management of Lepidopteran. . .
21
Table 2.2 LT50 values of NPV isolates against the second and third instar larvae
LT50 (h) values
Virus concentration Second instar larvae Third instar larvae
(POB/ml) HaNPV SlNPV AmalNPV HaNPV SlNPV AmalNPV
1.8 108 – – – 123.60 132.72 128.64
1.8 107 122.64 128.58 132.52 134.25 140.4 144.0
1.8 106 131.28 133.62 136.64 136.42 143.0 148.64
1.8 105 142.32 146.76 149.72 150.0 156.12 162.42
1.8 104 153.30 158.60 162.72 161.22 176.08 182.06
1.8 103 191.18 195.60 199.20 216.07 228.96 236.16
1.8 102 230.68 234.60 238.06 – – –
and are hyper-expressed during the very late phase of virus infection and are not
required for virus replication (Rohrmann 1986, 1992; Funk et al. 1997) and
constitute up to 18 % or more of total alkali-soluble protein late in infection
(Quant et al. 1984). It is a highly stable protein, insoluble in many solvents at
neutral pH values and physiological conditions, highly resistant against the action
of proteolytic enzymes, and at the same time it is highly sensitive to alkaline
conditions (Bergold 1947; 1948). At ICRISAT, with an aim of production of
polyclonal antibodies against poly-occlusion body protein (polyhedrin) for the
development of diagnostic and quality control tools during mass production of
NPVs, the purification protocol for polyhedrin protein was standardized (Sridhar
Kumar et al. 2007) with slight modifications to the methods suggested by Summers
and Egawa (1973), Harrap et al. (1977), and Quant et al. (1984). In 12 %
SDS-PAGE analysis, the denatured purified protein preparations of three viruses
resolved as single band (Fig. 2.6) with estimated molecular weights of 31.65 kDa
(0.00) (HearNPV), 31.29 kDa (0.00) (SpltNPV), and 31.67 kDa (0.295)
(AmalNPV). This report is similar to that reported by Tuan et al. (1999) for three
lepidopteran NPVs such as HaNPV, SlNPV, and SeNPV. Recently, Ashour
et al. (2007) reported the molecular weight of 32 kDa for recombinant and wild-
type A. californica NPV (AcAaIT and AcMNPV). In addition to the major
polyhedrin, they are contaminated with some minor low molecular weight peptides
of about 7–27 kDa and a high molecular weight peptide of about 60–70 kDa, which
could be the degraded peptides or dimmers of the 31 kDa polyhedrin protein. This
has revealed that these three NPVs have six to eight minor polypeptides.
To characterize NPV protein structure for the purpose of providing reliable
identification methodology and developing specific and sensitive serological detec-
tion techniques, the nucleocapsids of different HaNPV isolates across India were
purified by alkali dissolution of POBs followed by 25–60 % sucrose gradient
centrifugation (Sireesha 2006). Purified samples of HaNPV from ICRISAT, Uni-
versity of Agricultural Sciences of Dharwad, Tamil Nadu Agricultural University,
Panjabrao Deshmukh Krishi Vidyapeth in Akola, Punjab Agricultural University,
and Gujarat Agricultural University (UASD, TN, AK, PAU, and GAU) were
analyzed in 12 % SDS-PAGE gels for proteins. This has revealed that all the
Fig. 2.6 SDS-PAGE (12 %) profile of polyhedrin protein preparations of NPVs. Purified poly-
hedral protein (polyhedrin) preparations of NPVs were separated in 12 % SDS-PAGE. The
polyhedrin was appeared as single protein band in silver stained gel and the protein band at
~31 kDa was indicated with arrow mark. Lane 1: Protein molecular weight marker; Lane 2:
HaNPV polyhedrin; Lane 3: SlNPV polyhedrin; Lane 4: AmalNPV polyhedrin
isolates have 4 to 5 major polypeptides of 42.32 ( 0.92) kDa, 34.74 ( 0.27) kDa,
31.77 ( 0.44) kDa, 30.66 ( 0.27) kDa, and 19 ( 1.41) kDa and several minor
peptides. Three major proteins were present in all except in GAU isolate. The
molecular weights of the major proteins were nearly similar, but not identical. GAU
sample was unique in that it lacked the ca. 42 and ca. 34 kDa proteins. Several
minor proteins were also seen in the gel (indicated with arrows). GAU isolate
recorded one extra protein of 19 ( 1.41) kDa. It was also noticed in other isolates,
but it was not as conspicuous as in the case of GAU. Summers and Smith (1978)
studied the structural polypeptides of eight insect baculoviruses which revealed a
complex but unique composition of 15–25 bands with molecular weights ranging
from 15,000 to 1,60,000 Da. A. californica MNPV capsids contained two major
polypeptides VP18.5 and VP37; Rachiplusia ou MNPV capsids contained VP16,
VP18, VP30, and VP36; A. gemmatalis MNPV contained one major capsid protein
VP29; and the major capsid proteins of H. zea SNPV were VP16, VP28, and VP63.
Kelly et al. (1980) observed a high degree of similarity between the polypeptides
of two SNPVs of H. armigera and H. zea. Monroe and McCarthy (1984) charac-
terized the structural polypeptides of H. armigera NPV from India, China, and
USSR. For Indian isolate, the molecular weights of polypeptides ranged from 14.2
to 90.0 kDa. Harrap et al. (1977) purified polyhedron proteins from three closely
related insect pest NPVs, viz., S. littoralis, Spodoptera exempta, and Spodoptera
frugiperda, after dissolution in 0.05 M sodium carbonate and separation on 7 and
10 % SDS-PAGE. They did not observe significant differences between the sizes of
major proteins of the three viruses whose molecular weights ranged from 28 to
29 kDa. They also concluded that the smaller polypeptides of each virus preparation
represented an initial breakdown product derived by proteolytic cleavage of larger
molecule. Maskos and Miltenburger (1981) compared the polyhedral polypeptides
of L. dispar, M. brassicae, and A. californica NPVs using SDS-PAGE. They
observed eight distinct polypeptide bands with major polypeptides in the range of
28.0–30.0 kDa. They found characteristic differences between the species for minor
polypeptides having molecular weights in the range from 12.4 to 62.0 kDa. Cabal-
lero et al. (1992) observed similar mobility profiles of the occluded virion poly-
peptides and polyhedrins of four S. exigua NPV isolates originating from the United
States, Thailand, and two locations in Spain.
2.4.4 Efficacy of NPV Under Different Storage Conditions
Unlike chemical pesticides, viral pesticides often have a shorter half-life of infec-
tivity (Shieh 1978) that requires special attention for commercial operations. Viral
insecticides cannot be developed commercially until formulations of these are
physically, chemically, and environmentally stable in storage and distribution. At
ICRISAT, HaNPV storage studies were conducted over a period of time under
different set of storage conditions, and their efficacy was tested against the second
instar H. armigera larvae at the rate of 106 POB/ml at an interval of 2 months up to
10 months, and the mortality data on the 5th, 7th, and 9th days were analyzed using
two-way ANOVA. Variation in the efficacy was observed when stored under
different conditions of storage over a period of time (Table 2.3). NPV sample,
which was stored under refrigerated condition, could maintain its efficacy up to
8 months (100 %), and by the tenth month, there was a slight decline (97.50 %) but
it was not significant, whereas NPV sample stored in earthen pot and at room
temperature (both in amber-colored bottle and glass bottle) maintained its efficacy
up to 4 months and after that virulence started to decrease. This decreased efficacy
of samples stored under room temperature may be due to increased bacterial
activity. When the samples were tested for the bacterial load, it was 3.47 times
more in the samples stored at room temperatures after 6 months of storage. Gopali
and Lingappa (2001b) also recorded decreased efficacy of NPV when stored under
open house conditions, and it was opined that the change in the pH of viral
suspension stored under refrigerated condition was very slow from acidic to normal
(5–7) pH as against becoming excessively alkaline at ambient and earthen pot
conditions. It was also reported that this change was mainly brought about by the
growth of other microbes and warm conditions, which resulted in lowering of
virulence of viral bodies. Attathom et al. (1990) also reported the same.
The stability of HaNPV appears to be dependent on the resistance of the
inclusion body protein to decomposition. Many scientists (Stairs et al. 1981)
reported that the inclusion body protein is broken down by weak alkalies but it
Table 2.3 Efficacy of HaNPV under different storage conditions (on 9th day)
Percent larval mortality due to HaNPV (106 POBs) stored for different months
Storage conditions 0 2 4 6 8 10 Mean
Refrigerated 100 (71.56) 100 (71.56) 100 (71.56) 100 (71.56) 100 (71.56) 97.50 (68.11) 70.99
Earthen pot 100 (71.56) 100 (71.56) 100 (71.56) 95.00 (64.67) 92.50 (61.22) 87.50 (56.52) 66.18
RT (amb) 100 (71.56) 100 (71.56) 100 (71.56) 92.50 (61.22) 80.00 (52.79) 70.00 (48.90) 62.93
RT (glass bottle) 100 (71.56) 100 (71.56) 100 (71.56) 90.00 (57.77) 75.00 (51.82) 67.50 (48.03) 62.05
Control 5.00 (12.71) 5.00 (12.71) 7.50 (19.07) 5.00 (12.71) 7.50 (19.07) 10.00 (25.42) 16.95
Mean 59.7947 59.7947 61.0661 53.5892 51.2944 49.4025
Treatment Storage Interaction

SE 2.39 1.004 3.147
LSD 7.203 2.827 8.991
F (prob. at 5 %) <0.001 <0.001 <0.001
25
can withstand exposure to relatively strong acids and many other chemicals. Ebora
et al. (1990) reported that virulence was greatest around neutral pH and reduced
when subjected to high pH (12). Shapiro and Ignoffo (1969) showed that activity of
virions of HaNPV released from polyhedral cover lost about half of their activity
when stored for 60 days at 37 C, whereas virus particles covered with polyhedral
layer retain their activity for a longer period withstanding freezing and prolonged
normal field temperature than free virions (Yendol and Hamlen 1973). Many
scientists reported that virus could be preserved for more than 10 years at 4 C
without loss in virulence (Narayanan 1985). Gudauskas and Cannerday (1968)
found the thermal inactivation point of HaNPV to be 75–80 C for 10 min. The
virulence of virus depends on the quality of the virus, storage conditions, and
duration of storage, temperature, and pH of the product.
2.4.5 Effect of Chemicals on the Shelf Life of NPV
Information on screening of different chemicals or disinfectants on the shelf life of

NPV products is scarce. It is known that the NPV produced in live insects may
contain bacterial contamination (Podgwaite et al. 1983; Huber 1985) presenting a
potential health hazard. Although these studies on L. dispar NPV and C. pomonella
GV failed to detect human pathogens in the product, these viruses were produced
under temperate conditions, and it might be anticipated that production in a near
tropical situation would involve greater microbiological contamination problems.
Grzywacz et al. (1997) quantified microbial contaminants level in S. littoralis NPV.
They found 106–109 bacterial colony forming units/ml in virus suspension
containing 2.1 109 POBs/ml. They concluded that none of the bacterial contam-
inants found were harmful to cause potential health hazard. But some bacteria such
as Bacillus cereus might be of concern, from the point of view of standardizing the
product. Therefore, the development of production procedures, which reduce these
contaminants to a lower, more consistent level, would be valuable in promoting a
wider acceptance of viral insecticides as safe control agents. Simple centrifugation
in water does not remove many microbial contaminants, as bacterial spores, etc.,
tend to pellet with NPV OB. Grzywacz et al. (1997) suggested the use of bacteri-
ostatic agents and pH buffers to stabilize the formulations by reducing the multi-
plication of contaminants.
In our study, the HaNPV samples preserved in different chemicals over different
periods of storage varied in their efficacy and bacterial contamination levels
(Tables 2.4 and 2.5). The sample that was stored in distilled water maintained its
virulence up to 10 months as evidenced by the 5th, 7th, and 9th day mortality.
Cumulative mortality on the ninth day showed that the samples stored in 10 %
Dettol, 2 % phenyl, 10 % ethyl alcohol, and 10 % methanol recorded 100 %
mortality followed by 10 % acetone with 96.66 and 10 % ethyl acetate with 90 %
mortality after the period of 2 months. NPV sample stored in 10 % ethyl acetate
consistently reduced its efficacy as evidenced by the mortality on the 5th, 7th, and
Table 2.4 Effect of chemicals on the shelf life of HaNPV (on 5th day)
Mortality (%)
Treatment 2 4 6 8 10 Mean
NPV + 10 % Dettol 40.00 (39.23) 33.33 (35.22) 33.33 (35.22) 23.33 (28.78) 20.00 (26.57) 33.0025
NPV + 10 % acetone 73.33 (59.00) 63.33 (52.78) 60.00 (50.77) 56.66 (48.85) 53.33 (46.92) 51.6633
NPV + 2 % phenyl 50.00 (45.00) 43.33 (41.15) 43.33 (41.15) 40.00 (39.23) 36.66 (37.22) 40.753
NPV + 10 % ethyl acetate 40.00 (39.23) 40.00 (39.23) 40.00 (39.23) 33.33 (35.22) 30.00 (33.21) 37.2247
NPV + 10 % ethyl alcohol 53.33 (46.92) 53.33 (46.92) 53.33 (46.92) 50.00 (45.00) 50.00 (45.00) 46.1537
NPV + 10 % methanol 40.00 (39.23) 36.66 (37.22) 36.66 (37.22) 36.66 (37.22) 33.33 (35.22) 37.2247
NPV + distilled water 76.66 (61.22) 76.66 (61.22) 73.33 (59.00) 73.33 (59.00) 70.00 (56.79) 59.4474
Mean 47.12 44.82 43.22 41.90 40.13
Treatment Storage Interaction

SE 0.710 0.60 1.588
LSD 2.00 1.69 4.48
F (prob. at 5 %) <0.001 <0.001 0.169
27
Table 2.5 Effect of chemicals on the bacterial contaminants in storage

Before storage After a period of 6 months
No. of bacterial CFU/ No. of bacterial CFU/
Treatment colonies ml 106 colonies ml 106
NPV + 10 % acetone 20.33 1.01 19.66 0.98
NPV + 10 % ethyl 22 1.1 21.33 1.06
alcohol
NPV + 10 % ethyl 25 1.25 8 0.40
acetate
NPV + 10 % Dettol 23.33 1.16 25 1.25
NPV + 2 % phenyl 18 0.9 7.33 0.35
NPV + 10 % 22 1.1 24.66 1.23
methanol
NPV + distilled water 50.66 2.53 250 12.5
SE 0.433 0.02 0.349 0.01
LSD 1.335 0.06 1.075 0.050
F (prob. at 5 %) <0.001 <0.001 <0.001 <0.001
9th day. The results clearly indicated that, though all the chemicals could effec-
tively reduce the bad odor problem in storage, the samples stored in acetone and
ethyl alcohol only recorded 73.33 and 70.00 % mortality, respectively, by the end of
10 months. However, NPV samples in all the treatments gave 90 % mortality after
storing for a period of 2 months (Tables 2.3 and 2.4). Ignoffo and Shapiro (1978)
suggested the use of acetone in purification of NPV POBs. Acetone, being a
potential antimicrobial agent, regulates bacterial infection and, being a lipid sol-
vent, removes the lipid (fat cells) from the larval homogenate, thereby inhibiting
bacterial lipid degradation and in turn the malodor. To address the problem of
bacterial contaminants, Rao and Meher (2004) used 10 % acetone solution. They
could clear the lipid mass and leftover contaminating bacteria in the preparation and
regulated the malodor problem. They confirmed the viability of the virus after
1 month of acetone clarification by conducting bioassay studies.
2.4.6 Molecular Characterization
Molecular-level identification, characterization, and evaluation of phylogenetic

status of a particular baculovirus are also important for the establishment of purity
of seed stock or master stock. Apart from the multiple or singly enveloped feature,
NPV or GV cannot be identified visually from either light or electron microscopic
studies. Microscopic and serological tools are unreliable for establishing the real
identity of a given isolate and are not particularly helpful in providing clues about
its host range and infectivity (Rovesti et al. 2000). Differences between viruses are
usually reflections of intrinsic differences in their viral genomes. At one extreme,
viruses may be readily distinguished by the nature of the nucleic acid (RNA or
DNA) and its strandedness (single or double stranded), while more closely related
viruses may differ only by small regions of distinct base sequences which can be
defined only by more sophisticated techniques such as restriction endonuclease
(REN) analysis or molecular probes, or sequencing of conserved and unique gene
sequences which offers a relatively simple method for the identification and
differentiation of baculoviruses (Smith and Summers 1978).
The only nucleic acid type found within the enveloped nucleocapsids of these
viruses is a dsDNA molecule. DNA of baculoviruses is a large circular molecule.
REN analysis can provide a measure for baculovirus DNA molecular weight. It has
a more useful role in virus identification and ultimately in mapping of the viral
genome. Examination of the DNA using these techniques has shown that many
variants of a species may exist, for example, the MNPVs from A. californica,
Trichoplusia ni, S. exempta, R. ou, A. falcifera, and Galleria mellonella can be
considered to be variants of the same virus (Miller and Dawes 1978; Smith and
summers 1979; Summers et al. 1980; Brown et al. 1984; Harrison and Bonning
1999). Many of the known baculoviruses could be grouped together depending on
their degree of genetic relationship, which does not reflect the taxonomic grouping
of their host/hosts (Zanotto et al. 1993). Among the NPVs with potential as pest
control agents, the MNPVs isolated from M. brassicae (Lepidoptera: Noctuidae)
and H. armigera (Lepidoptera: Noctuidae) were shown to be similar in terms of
both biological activity and genomic homology (Smith and Summers 1982; Figuei-
redo et al. 1999; Rovesti et al. 2000). For identification of a particular NPV strain,
the bioassay studies and REN profiles of different NPV species have been studied
and compared by several investigators (Shapiro and Ignoffo 1970; Hughes
et al. 1983; William and Payne 1984; Rabindra 1992; Somasekhar et al. 1993;
Arora et al. 1997; Geetha and Rabindra 1999; Sudhakar and Mathavan 1999;
Figueiredo et al. 1999; Rovesti et al. 2000). The existence of genetic variants
with different biological activities may have important implications for develop-
ment of bio-pesticides both in the possibility to select better naturally occurring
strains and as a source material for genetic manipulation (Guo et al. 2006).
In order to establish the purity of seed stock or master stock of HaNPV used for
commercial insecticide preparations, an attempt was made at ICRISAT by molec-
ular characterization of HaNPV done by isolation, cloning, sequencing of
polyhedrin gene, and evaluation of the phylogenetic status (Sridhar Kumar 2008).
Considering the sizes of previously published polyhedrin gene sequences, most
amplification products were between 730 and 750 bp long. Gene sequencing
analysis of selected clones resulted in 744-nucleotide-long ORF with a predicted
coding capacity for a polypeptide of 247 amino acids as in the AmalNPV
(AF118850). Similarly, Rivkin et al. (1998) reported a 246 amino acid polypeptide
in a local strain of Israeli HaNPV polyhedrin, and Bansal et al. (1997) reported
same length of amino acid polypeptide in SlNPV polyhedrin. The polyhedrin is the
major protein of the virus OB and is the most conserved protein of NPVs
(Rohrmann 1992). After the first report about localization of the polyhedrin gene
in AcNPV, its nucleotide sequence was determined (Vlak and Smith 1982; Hooft
van Iddekinge et al. 1983). Polyhedrin/granulin is a protein of about 245–250 amino
acids and appears to be the most highly conserved baculovirus protein. These
characteristics lead to the use of polyhedrin or granulin sequences as the base of
baculovirus phylogenetic studies, since this is the gene from which a larger number
of different examples are available for comparison (Zanotto et al. 1993). Although
polyhedrin gene is still considered a reasonable marker for identification of an NPV
and its neighbors, Herniou et al. (2003) and Lange et al. (2004) argued that it might
not be the best baculovirus gene for phylogenetic studies because polyhedrin
phylogenies often disagree with other gene phylogenies. While other phylogenetic
analyses consistently group AcMNPV and BmNPV together, phylogenies based on
polyhedrin have AcMNPV as a sister group to the rest of the group-I NPVs
(Herniou et al. 2003). Phylogenies based on combined sequences of shared genes
have been found to be more robust than those based on the sequences of individual
genes (Herniou et al. 2003).
PCR, when combined with the use of REN analysis, can provide considerable
resolution for use in diagnostics; it is relatively simple to use and can provide quick
results. Not surprisingly, this approach is now widely used for detection and
identification of a range of insect viruses (Kool et al. 1991; Williams 1993; De
Moraes and Maruniak 1997; Bulach et al. 1999). Christian et al. (2001) developed a
rapid method based on PCR–RFLP analysis for identification and differentiation of
HaSNPV and AcMNPV groups by using a set of redundant primers to highly
conserved region of polyhedrin gene. Based on REN analysis, Rovesti
et al. (2000) reported that the HaNPV isolate was shown to be a mixture of many
closely related genotypes but individual genotypes remained unchanged on passage
in either H. armigera or M. brassicae. Doyle et al. (1990) noted that when
MbMNPVD was passed in Orthosia cruda, there were minor changes in the
restriction enzyme profile, which was attributed to the selection of a different
variant. However, bioassay studies of Rovesti et al. (2000) showed that the two
viruses HaMNPV and MbMNPV were successfully replicated in H. armigera,
M. brassicae, and H. zea, resulting, in each case, in progeny virus which was
essentially similar to the inoculum. Therefore, a viral insecticide based on these
NPVs from H. armigera and M. brassicae would be more appropriately targeted
against both insects. Similarly, Murillo et al. (2001) have reported that REN profiles
of two SeNPV isolates (SeUZB and SeSP3) in Uzbekistan and Spain and MbNPV
(Mb-PL) in Poland were closely related to previously described Spanish isolates of
SeNPVs. At the same time, the Pst-I and Bgl-II profiles of SeUZB and Mb-PL were
identical and very similar to the REN profiles of MbMNPV strain which is the
active component of MamestrinR, a commercial bio-insecticide in France (NPP,
Nogueres, France). In addition to SeMNPV, S. exigua is susceptible to other NPVs
such as A. californica MNPV (Smits and Vlak 1987) and MbMNPV (Wiegers and
Vlak 1984). In another case, AcMNPV and BmNPV also show a high degree of
genomic homology and different REN fragment profiles but do not share an
overlapping host range and can be regarded as two different species. It is interesting
to note that only minor changes in the virus genome, namely, one or two amino acid
substitutions in the AcMNPV helicase p143, are sufficient to expand the host range
of AcMNPV to Bombyx mori larvae (Kamita and Maeda 1997; Arguad et al. 1998).
Clearly, there are problems in naming a baculovirus simply on the basis of the
host from which it was originally isolated, and therefore, greater vigilance should
be taken in naming new (and old) viruses. For example, studies on viruses from
members of the same or different host species show similarity or variability in
morphology, virulence, and biological characteristics (Shim et al. 2003). Many of
the known baculoviruses could be grouped together depending on their degree of
genetic relatedness, which does not reflect the taxonomic grouping of their host/
hosts (Zanotto et al. 1993). Based on the above reports, one can comment like
“variants of baculoviruses with heritable similarities in virulence and variations in
host range arise spontaneously in nature.” In the earlier days of baculovirology, it
was believed that baculoviruses could only infect a single host species and that no
cross-infection occurred. This generalized the use of binomial Latin names of the
insect species hosts to describe the new viral isolates. However, this practice can
affect our understanding of baculovirus biology and can also lead to confusion upon
studying the classification and taxonomy of baculoviruses (Federici and Hice 1997)
and should be changed by more reliable methods based, for instance, on the
genotypic characteristics of the viruses. A useful means for identification or
description of baculoviruses is REN analysis of viral DNA, as firstly demonstrated
by Lee and Miller (1978). This method has proved to be very useful not only for
distinguishing distinct NPV species but also different strains of one virus or even
different genotypes within the same virus isolate (Smith and Crook 1988; Munoz
et al. 1998). In general, baculovirus wild-type populations, from different geo-
graphical isolates of the same virus and within a single isolate, where several
genotypic variants frequently coexist, show a considerable genetic heterogeneity.
This heterogeneity is due to the enormous plasticity displayed by baculoviruses
genomes which can undergo deletions (Munoz et al. 1998), insertions (Jehle
et al. 1995), point mutations, recombinations (Croizier and Ribeiro 1992), etc.
This plasticity suggests that field isolates may be adapting to host and environmen-
tal conditions and that those isolates containing heterogeneous populations may be
more valid for viral survival in the field (Possee and Rohrmann 1997). Investiga-
tions of geographic variability and the role of genotypic differences in the biology
of baculoviruses are an important area of current research. Such studies may
provide insight into the evolution of baculoviruses and their hosts and may also
aid in the development of more effective virus strains for biological control of
insects.
2.5 Production Technologies
Historically, several entomopathogenic viruses have been produced in susceptible

host insects, because of the following reasons: (1) The insect host is an efficient
virus producer (Ignoffo and Couch 1981). (2) Automation of in vivo rearing and
in vivo production systems is feasible (Powell and Robertson 1993). Some
baculovirus species may be produced in insect cell cultures, but the associated
costs are relatively high (Hink 1982). Therefore, all NPVs that have been developed
as commercial products thus far have been produced in host larvae.
2.5.1 In Vivo Mass Production
In vivo virus production has several advantages like (1) Successful use of viruses
produced in the host to control insect pests (Ignoffo and Couch 1981; Bell 1991).
(2) Research is continuing in this area to produce more efficient systems, which
makes this approach an economically viable one. (3) In many areas of the world,
virus production in the host is the only approach feasible (Katagiri 1981; Moscardi
et al. 1981). In vivo mass production systems have changed little over the past
30 years. The development of semi-synthetic artificial diets by Vanderzant
et al. (1962) resulted in rearing and virus production systems for the cotton
bollworm (H. zea), the tobacco budworm (Heliothis virescens), and the cabbage
looper (T. ni) by Ignoffo (1965). The initial rearing system was made more efficient
by the introduction of disposable multi-celled plastic trays (Ignoffo and Boeing
1970), automation in rearing, and automation in virus inoculation and harvesting.
Optimal virus production is the result of interrelationships of host–pathogen–
environment, and each factor in this triad must be assessed for influence on quantity
and quality of the product. Research in these areas has been summarized (Shapiro
et al. 1986; Shapiro and Bell 1981, 1982). A broader and more complete account of
some aspects on virus production and role of virus in insect pest control has been
given by Burges (1981). Subsequent development and industrialization for mass
rearing process, improvements in viral recovery procedures, and formulation of the
viral product made it possible for commercialization of HaNPV (Shieh 1978).
Further, Ignoffo and Couch (1981) improved the method of mass production of
baculovirus of Helicoverpa from the laboratory-reared Helicoverpa larvae through
which 7–9 times more active virus and 2–5 times more POBs were obtained from
dead and diseased larvae.
Field collection of diseased larvae led to contamination with adventitious agents
which would pose a major problem in terms of safety and quality control, and as
such, it was not desirable for HaNPV production (Sherman 1985). Because of the
developments in semi-synthetic diet, containerization, and automation, laboratory-
reared insects have been the hosts of choice. The advantages of these insects are
(1) Laboratory-reared insects tend to be larger than field-collected insects, because
of the selection and adaptation to the laboratory environment (diet, temperature,
humidity, and photoperiod). (2) They are normally disease free, which should result
in virus product that is free from other pathogens. (3) The growth and development
of laboratory-reared insects tend to be faster than field insects, because of selection.
(4) Virus yield among laboratory-reared insects tends to be greater than among field
insects, since virus yield is dependent on host biomass (Hedlund and Yendol 1974;
Shapiro and Bell 1981). Although laboratory-colonized insects provide several
advantages over field insects as virus producers, field insects have also been used
successfully to produce NPV from larvae of the potato tuber moth (Phthorimaea
operculella [Zeller]) in Australia (Matthiessen et al. 1978), the velvetbean cater-
pillar (A. gemmatalis) in Brazil (Moscardi et al. 1981), and the European pine
sawfly (N. sertifer) in the United States (Rollinson et al. 1970) and CPV from the
pine caterpillar (Dipodomys spectabilis) in Japan (Katagiri 1981) on natural foliage.
Different methods of mass production of baculoviruses, according to Pawar and
Thombre (1992), are (1) large-scale rearing of insects in the laboratory, (2) field
collection of host larvae from infested crops and infecting them in the laboratory,
and (3) field collection of diseased larvae from infested fields. Large collection of
insect viruses at the rate of 20,000 host larvae have been reported from different
crops, viz., cotton, sunflower, pigeonpea, and chickpea (Ignoffo 1966a, b; Anderson
et al. 1972; Battu 1992). Battu (1992) reported relatively lower levels of POBs
obtained from field-collected, diseased, and dead insects. The number of larvae
required to produce one larval equivalent (LE) (6 109 POBs) of virus from field-
collected larvae was higher (2.97) than laboratory-reared ones (2.14) since field-
collected larvae were of different sizes unlike the uniform stages in the laboratory-
reared ones (Gopali and Lingappa 2001a). At ICRISAT, for effective mass multi-
plication of AmalNPV, the field-collected larvae are released into an aluminum or
polythene grid/enclosure (10 cm height) to confine the larvae inside the shaded
enclosure and feed with plants already inoculated with the virus. The field tech-
nique for rearing larvae is advantageous, particularly in avoiding the handling of
huge larval populations, rearing, and inoculation. This would also facilitate farm-
level production and access to the bio-pesticide at the village level (Rao 2006). The
laboratory-level mass production technique for AmalNPV has been standardized by
Veenakumari et al. (2006). In situ field-level mass production of AmalNPV in a
groundnut ecosystem was developed for the first time at Project Directorate of
Biological Control (PDBC), Bangalore, India (Veenakumari et al. 2007).
The virus used for the inoculation must confirm the quality control specifications
of viral products as reported by Shieh and Bohmfalk (1980). The inoculation dose is
expressed in units of POB/ml, and the optimal dose varies with the virulent virus
and age of the host (Ignoffo and Couch 1981). Angelini and Labonne (1970)
suggested that the best method to propagate the virus was to spray a suspension
on larval diet. They could get the larval mortality after 7–8 days. Shapiro and Bell
(1981) reported that surface treatment is an efficient system that is easily automated
and requires much less virus than diet incorporation. However, Odak et al. (1984)
used soaked chickpea seeds treated with HaNPV to feed Helicoverpa larvae and
found that the method was effective for mass production of virus. Bioassays were
used to determine the activity of each batch of virus. Several modes of administra-
tion of virus were tried using different larval instars, viz., surface treatment, diet
incorporation, and direct feeding (Ignoffo 1966a). Earlier instars were highly
susceptible to the virus (Rabindra and Subramanian 1974) with LT50 shorter than
older ones. Narayanan (1979) report that the early instars recorded 100 % mortality,
whereas the late instars particularly from the fifth instars pupated and gave rise to
malformed adults with short and ruffled wings. The effect of NPV was directly
related to the age of the larvae at the time of infection (Battu 1990). Further, Battu
(1992) reported that increasing dosages are required to kill the older larvae. The
relative resistance of 8-day-old larvae was 2,000 times more than that of 1-day-old
larvae. Further, Battu observed that the fifth and sixth instar larvae could not be
infected with the virus even at higher concentrations. Rabindra and Subramanian
(1974) inoculated the fourth instar larvae with a dose of 106 POBs/ml to harvest
maximum yield. The LC50 values for the first and third instars of H. armigera were
8.3 103 and 28.6 105 POBs per larva, respectively (Backwad 1979). Narayanan
(1979) found that the optimum dose of inoculum required for obtaining maximum
harvest of virus from the fourth instar larvae was 5 104 POBs/cavity/larva by the
diet surface contamination method, whereas Shieh (1978) used 5 105–5 106
POBs/ml inoculum in each cavity and observed that there was significant interac-
tion between the age of the larvae and dose of the virus with the recovery of POB.
Taun et al. (1989) described the pathogenicity of HaNPV to H. armigera using
three different inoculation methods. The LD50 values of the fourth instar larvae that
were fed on diet containing NPV or maize kernels soaked in virus suspension were
1.85 106 and 2.55 105 POBs per larva, respectively. The inoculum-imbibing
method was more sensitive and convenient for inoculating the pest with virus,
whereas Jayaraj and Sathiah (1993) described the three methods of inoculation,
viz., head dipping, oral feeding, and diet surface contamination, and the latter
method was the most economical and convenient for easy application. Ignoffo
(1966b) estimated that at least 6 109 virus polyhedra were produced per larva in
late instars of H. zea, and he defined it as “one larval equivalent.” The average yield
of virus per larva infected after 5–7 days at 30 C was 1.5 109 polyhedra (Ignoffo
1973). Teakle et al. (1985) observed that the least yield of 1.18 107 POBs/insect
was from younger larvae of H. armigera compared to 3.6 109 POBs/insect from
grown-up larvae, whereas Shieh (1978) recovered 5 109 POBs/larva, indicating
that the yield of POBs was directly related to the age of the infected larvae. The host
insect, insect diet, insect age and virus dosage, incubation, environment, and
preservation of virus infectivity were some of the major factors that optimize the
production of HzNPV (Carter 1984). The virus yield increased exponentially with
the age of larva at dosing in the range of zero to six days, the overall increase being
approximately 100-fold (Teakle and Byrne 1989). Battu (1990) reported an average
yield of 1.81 109 POBs per larva of H. armigera. Similarly, Pawar and Thombre
(1992) reported that HaNPV yields per larva ranged from 0.95 109 to 3.5 109.
Gopali and Lingappa (2001a) suggested 108 POBs/ml as the optimum dose required
for the third and fourth instar larvae to achieve quicker and higher mortality of
larvae for virus production, and among different instars of H. armigera, the fourth
instar larva was found ideal for virus production as it yielded higher quantity of
virus per larva (2.81 109 POBs). In vivo mass production and control efficacy
studies of S. litura NPV (SlNPV) were positively correlated with larval weight from
the third instar to the fifth instar larvae; a maximum yield of 1.4 109 POBs/ml was
obtained with the early fifth instar larvae individually infected by diet-incorporation
of inocula of 3 106 POBs/ml for 7 days of incubation at 30 C (Tuan et al. 1998).
Similarly, a maximum yield of 5.57 109 POBs/larva was obtained at the inoculum
dose of 1966.2 POB/mm2 of S. litura NPV when exclusive harvest of cadaver was
done (Senthil Kumar et al. 2005). Jun et al. (2007) reported that the volume of POBs
of SlMNPV harvested on the 5th day of the postinoculation period was significantly
lower than that harvested on the 7th day of the post-incubation period which was
significantly lower than that harvested after larval death, and a similar trend was
observed in biological activity by dosing the fifth instar larvae. To study the
influence of virus inoculation method and host larval age on the productivity of
the NPV of the teak defoliator, Hyblaea puera (Cramer) was determined by
different methods of inoculation (Biji et al. 2006).
At present in India, in vivo propagation is being practiced for NPV mass
production at commercial scale and even at farmer level. Healthy larvae reared in
the laboratory or collected from the fields are fed with low dose of NPV, and the
virus produced in the insect is harvested and its concentration is estimated by
counting POBs using a light microscope fitted with a hemocytometer. Recently,
local production and utilization of NPV gained momentum in India through partic-
ipation of scientists, farmers, NGOs, and state agricultural and extension depart-
ments. In the fields, natural mortality of Helicoverpa and Spodoptera can be seen
due to infestation of disease-causing virus particles. Such larvae can be collected
and may be utilized for in vivo mass propagation and again for checking their
efficacy against pest populations. ICRISAT (Rao et al. 2007) trained several
national agricultural research and extension systems (NARES) scientists and
farmers on bio-pesticide production and established 96 village-level NPV produc-
tion units in India and Nepal to encourage their use. As the selection of virulent
strain of NPV is key to the development of effective bio-pesticides, local strains are
always preferred for sustainability, adaptability, and efficacy under a given set of
agroecosystem and hold an ample scope for their widespread multiplication and
commercial use in a particular region (Gupta et al. 2007, 2010). It is well recog-
nized that factors such as the geographic origins of both the virus and host can affect
the characteristics of the dose–response curve and the period of survival of infected
hosts (Maeda et al. 1990). For production of HaNPV and SlNPV, a host insect larva
has to be multiplied on artificial or semisynthetic diet or soaked chickpea seeds.
Crude HaNPV is commercially produced at Dr. Panjabrao Deshmukh Krishi
Vidyapeeth, Krishi Nagar, Akola (Maharashtra), and at Agricultural Research
Station, Gulbarga (Karnataka), by following the procedures: host insect multipli-
cation, virus inoculation and harvest, extraction and purification of virus, and
standardization of NPV.
2.5.2 Problems Associated with Commercialization of In

Vivo-Produced NPV Products
In vivo mass production of NPVs is labor intensive and involves mass rearing and
infection of insect larvae, which account for high production costs. In addition, the
products have some quality and storage issues which severely affect the efficacy
and quality of the products. One of the major problems involved in harvesting virus
from dead larvae was that they were often heavily contaminated with bacteria.
Purification methods based on centrifugation were found to be less effective in
removing bacteria (Sireesha 2006; Sridhar Kumar 2008). Other problems are
inconsistency in the yield and malodor during production and even in the finished
product also. Another important problem associated with the commercialization of
NPV products is the lack of proper diagnostic systems to quantify the virus titer,
microscopic counting procedure used to screen the larvae for NPV infection, and
quality control of the viral insecticide batches which has low-detection efficiency,
unknown specificity, and is laborious and requires considerable skill (Wigley
1976). Because of this, many NPV products produced in India have poor efficacy
and are found to be ineffective under field conditions. To overcome this problem
and for effective production of viral insecticides, it is necessary to have an efficient
strategy for virus production, combined with rapid and specific diagnostic and
quality control system (Shieh 1989).
Microbial pesticides including NPVs and GVs have now been brought under the
ambit of the Central Insecticide Act, 1968. Commercialization of microbial pesti-
cides is possible only after registration with the Central Insecticide Board (CIB) in
India, a body constituted under the insecticides act that regulated their manufacture
and use. Section 9(3b) of the act specifies a minimum quantity of active ingredient
in the formulation. Many of the viral products available in the markets in develop-
ing countries were classified as weak, with poor efficacy, questionable quality
control (Harris 1997), and are failing to meet acceptable standards (Kern and
Vaagt 1996). Unless this matter is addressed effectively, there is serious danger
in these countries that poor quality products with their inevitable failures will erode
the farmer’s confidence in microbial control products such as NPV and significantly
retard the promotion of this potential technology. There has been a rapid growth in
the production and use of NPV products in the last decade in India, but this has
exacerbated quality problems (Kambrekar et al. 2007). The causes of poor quality
lay in deficiencies in production technologies and poor quality control procedures.
NPVs of Helicoverpa and Spodoptera have been most extensively researched and
studied with regard to their efficacy, mass production, and compatibility with
botanicals and other insecticides and against several nontarget organisms
(Hunter-Fujita et al. 1998 and Saxene and Ahmad 2005). Pathogenicity of the
isolates varies according to localities and therefore needs to be screened. Develop-
ment of economical in vitro cell culture techniques for large-scale production of
NPVs which will go a long way has not yet been developed for agricultural use.
2.5.3 Is In Vitro Production the Only Solution to Address

Quality Control Issues?
While several improvements in in-vivo production systems in insect larvae have

been made in the past, these could not help to overcome the problems described
above (Sireesha 2006; Sridhar Kumar 2008). Hence, it has been proposed that the
adoption of an alternative technology based on the viral propagation in insect cell
cultures could help to overcome the problems associated with in vivo technology
and enable the development of well standardized, controlled, and scalable produc-
tion processes for insecticidal baculoviruses (Szewczyk et al. 2006). In the early
part of the twentieth century, entomologists had a dream of utilizing insect cells
grown in vitro as a tool for producing entomopathogens. These early experiments
used a simple saline solution or hemolymph as the culture medium, and cultures
could rarely be kept for more than a few days. A breakthrough occurred four
decades ago when Grace (1962) successfully established long-term cultures of
insect cells. Since then, over 500 continuous cell lines have been established
from over 100 insect species (Lynn 1999). Insect pathologists have cells capable
of replicating dozens of insect-specific viruses (Granados and McKenna 1995),
while plant pathologists and vertebrate pathologists have cells capable of replicat-
ing viruses transmitted by insects (Mitsuhashi 1989). Mass production of the virus
at reasonable costs is an important factor in the development of NPVs into a
marketable product.
2.5.4 In Vitro Production
Baculoviruses can be produced in vitro in infected insect cells cultivated in bio-

reactors. In order to develop an economically feasible process to produce
baculoviruses in insect cells, low-cost culture media that satisfy the nutritional
demands of both uninfected and infected cells are needed to achieve high virus
yields. Fetal calf serum is the most widely used additive in insect cell culture media.
However, its high cost and batch-to-batch variability are the drawbacks in its
utilization to sustain baculovirus production in large-scale processes. Therefore, a
replacement for the fetal calf serum in insect cell culture media is the key step to
develop a technically and economically feasible process to produce baculoviruses
in vitro. Recent studies demonstrated that insect’s cells are able to both proliferate
permanently and replicate baculovirus in a lipid-free environment. However, in
order to obtain a useful medium for technological applications, it will be necessary
to optimize the composition of the multiple supplements and evaluate its perfor-
mance in a lipid-supplemented environment. Insect cell cultures have been exten-
sively utilized by Linda and Lua Steven Reid (2003) for means of production for
heterologous proteins and bio-pesticides. Spodoptera frugiperda (Sf9) and T. ni
(High Five™) cell lines have been widely used for the production of recombinant
proteins; thus, metabolism of these cell lines has been investigated thoroughly over
the recent years. NPV isolated from the alfalfa looper, A. californica, was replicated
successfully and rapidly in a suspended ovarian cell line of the cabbage looper, T. ni
(Vail and Jay 1973). Polyhedra were observed in the nucleus of cells within 20 h
after inoculation. The cyto-pathological changes typical of nuclear polyhedrosis
infections were observed, and an average of 64 polyhedra/cell was produced. These
polyhedra were quantitatively as infectious to cabbage looper larvae as those
produced in vivo. In addition, they were infective to H. virescens, Pectinophora
gossypiella, S. exigua, A. californica, and A. falcifera. Bioassays have indicated that
both H. zea and H. armigera viruses produced in vitro maintain biological activity
(Suzanne 2009 and Szewczyk et al. 2006). Chakraborty et al. (1999) studied the
in vitro production of virus from H. armigera (HaSNPV) and its possible use as a
specific Helicoverpa/Heliothis larvicide. Growth kinetics of H. zea cells and virus
OB yields were compared in three SF900II-based media, namely, SF900II (serum-
free), SF900II +1 % serum, or SF900II +10 % serum. Viable cell densities were
usually higher in the media supplemented with serum than in the serum-free
medium; however, in the serum-free medium, cell diameters were 1.7 times greater
(i.e., individual cell volumes were five times larger). Four new cell lines, designated
as NTU-LY-1 to 4, respectively, were established from the pupal tissues of
Lymantria xylina Swinhoe (Lepidoptera: Lymantriidae) (Wu and Wang 2006).
These cell lines have been cultured approximately 80 passages during 2 years in
TNM-FH medium supplemented with 8 % fetal bovine serum, at a constant
temperature of 28 C. Each line consists of three major morphological types:
round cells, spindle-shaped cells, and giant cells.
Sundeep et al. (2005) developed two cell lines from the larval hemocyte and
embryonic tissue of H. armigera and designated them as NIV-HA-1195 and
NIV-HA-197, respectively. The NIV-HA-197 cell lines were found highly suscep-
tible to HaSNPV, yielding a very high titer (2.88 107 NPV/ml) on the 10th
postinfection day (PID). The HaSNPV OBs produced in vitro were highly virulent
to the second and third instars H. armigera larvae causing cessation of feeding on
the 2nd day and mortality in 6 days. This cell line is also found to be growing well in
goat serum (GS)-supplemented medium producing a comparable yield of OBs.
Goat serum, being cheap and locally available, will help in the large-scale produc-
tion of HaSNPV for use as a biopesticide in the future. The cell line NIV-HA-197
was found to be susceptible to the baculoviruses AcMNPV, SlMNPV, and HaSNPV
(Sundeep et al. 2002). More than 90 % of the cells were infected by HaSNPV on the
seventh PID, and 28.8 106 NPV/ml was yielded on the 10th PID. The in vitro-
grown HaSNPV caused 100 % mortality, when fed to the second instar H. armigera
larvae, in 6 days. Isoenzyme profile and results of 16S rRNA heteroduplex analysis
clearly indicated the species specificity of the new cell line NIV-HA-1195
(Sundeep et al. 2002) and was also found susceptible to the baculoviruses,
AcNPV, SlNPV, and the homologous HaNPV. Pant et al. (2002) reported that the
H. armigera cell line from the embryonic tissue was highly susceptible to HaNPV
(6.3 106 NPV/ml). These in vitro-grown HaNPVs caused 100 % mortality in the
second instar larvae. The cultures could grow as suspension culture on shakers and
may find application for the in vitro production of wild-type/recombinant

baculoviruses as bio-insecticides.
Nakat (2004, In vitro production of nuclear polyhedrosis virus of Helicoverpa
armigera and Spodoptera litura and its field efficacy in Western Maharashtra.
Department of Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharash-
tra, unpublished) standardized the procedure for monolayer and spinner culture of
Sf-9, Sf-21, and Ha-197 cells. The growth curve of different cells in spinner culture
was plotted on the basis of daily viable cell count. The cell line Sf-9 was susceptible
for both the baculoviruses AcMNPV and SlNPV in monolayer. The cell line Ha-197
was found susceptible for HaNPV in monolayer. For production of HaNPV, Ha-
197 cell line with HaNPV wild-type MPKV strain was found to be efficient, more
virulent, and infectious in both the cell lines. The procedure for the cell lysis was
standardized to extract the POBs from infected cells with the addition of 0.1 % SDS
and deep freezing at 20 C followed by 15 min sonication resulting in good
separations of POBs from 80 to 90 % infected cells. The field demonstrations of
in vitro- and in vivo-produced SlNPV and HaNPV were conducted on capsicum,
gerbera, rose, soybean, and chickpea. The effectiveness of in vitro-produced NPVs
was found to be superior, causing larval mortality in the range of 78–100 % as
compared to in vivo-produced virus which was 70–88 %.
The insect cell line, the culture medium, the bioreactor, the virus, the infection
parameters, and the culture strategy are elements of the insect cell culture technol-
ogy that must be optimized in order to develop in vitro production processes for
insecticidal baculoviruses (Claus Juan et al. 2012). The cell line Hz-AM1 has been
used widely to examine possible factors affecting the yields and the potency of
HaNPV. These factors include the medium, supplemented serum, cell density at
infection, multiplicity of infection, viral strain, and passage effect (Chakraborty
et al. 1996; Lua and Reid 2000; Ogemo et al. 2007). Increasing OB yields per cell in
culture is the main challenge to enable commercialization of in vitro production of
NPVs. Isolating clones from a heterogeneous cell population may allow develop-
ment of a high virus-producing cell clone. An automated robotic clone-picking
system to establish over 250 insect clones of an H. zea cell population to be
screened for virus production has been carried out by Nguyen et al. 2011. The
type and degree of passage effect are dependent on the cell lines and the virus
species (Krell 1996). Homologous cell lines are desirable for the production of an
NPV, whereas heterologous NPV infection of cell lines decreases the productivity
and yields less virulent progeny viruses (Tompkins et al. 1988).
Although the production of insecticidal baculoviruses in insect cell cultures has
been proposed as an alternative to overcome the limitations of the in vivo processes,
so far no in vitro process could be even implemented on an industrial scale and
baculovirus occlusion bodies are still produced in infected insect larvae (Claus Juan
et al. 2012). Some factors that 25 years ago have hindered the development of large-
scale production processes for baculoviruses in insect cell cultures, such as the
sensitivity of insect cells to the stresses linked to the mechanical agitation in stirred
tank reactors and to the bubble rupture in sparged bioreactors, have been resolved,
and several cell lines can be cultivated today in industrial bioreactors of large
volume to produce occlusion bodies or recombinant proteins.
In vitro propagation in susceptible insect cell lines is the best option for the
commercial production of this virus. Recently, NIV (Pune) developed indigenous
cell lines from four insect species, and their susceptibility to different NPVs was
tested, and are commercially selling them to interested scientists (Pant et al. 2002).
The cultures can grow as suspension culture on shakers and are found to be
successful for in vitro production of wild-type/recombinant baculoviruses as
bio-insecticides. However, most cell lines have not been sufficiently characterized
with respect to certain issues such as (1) simplification of the composition of the
culture medium, (2) possibility to obtain high volumetric yields of viral OBs, and
(3) optimization of seed virus or budded virus or nonoccluded virus. Production
related to economic feasibilities for entrepreneurs needs to be addressed.
2.6 Diagnostic and Quality Control Tests
The reliability of the product is crucial in ensuring acceptance and sustained use by
the farmers. The issue of erratic performance of viral biocontrol agents has been
recognized as a significant factor in limited successful commercialization (Lisansky
1997). It has been widely perceived that viral agents have not achieved a level of
efficacy comparable with that of chemicals or other bio-pesticides such as Bacillus
thuringiensis (Berliner).
Mass production of NPV insecticide is simple and widely produced even at
farmer level. Although commercial production, quality, and storage were still
contentious issues, NPV is multiplied on field-collected larvae and being applied
on crops. Multiplying NPV on field-collected larvae was found to be easier and
cost-effective compared to laboratory-reared larvae, but efficacy and quality of
which may be affected due to contaminants such as bacteria and fungi.
The effectiveness of the viral insecticide is critically dependent on the concen-
tration of POB, which is expressed as LE. Generally, a standard stock preparation
consists of 1LE, i.e., 6 109 POBs/ml. NPV production methods have been well
established in many developing countries. Appropriate, sensitive, and reliable
serological tools (Kohler and Milstein 1975; Kelly et al. 1978; Towbin
et al. 1979; Crook and Payne 1980; Smith and Summers 1981; Zhang and Kaupp
1988; Lu et al. 1995) are not available at this stage and will go a long way in the
quality control of insect viruses in developing countries. Once developed, the tools
would be of immense value to public and private entrepreneurs, such as state
bio-pesticide production laboratories and regulatory agencies. In addition to this,
the highly standardized, accurate, and sensitive diagnostic tools for NPV detection
in field-collected larvae would be beneficial to pest management personnel, because
early detection of NPV disease could make it possible to predict the occurrence of
an imminent epizootic and thus alter the pest control tactics to be employed
(Volkman and Falcon 1982).
As part of quality control during mass production of NPVs used for commercial
viral insecticide preparations at ICRISAT, polyclonal antibodies were raised
against purified polyhedrin (POB) protein preparations and used these antibodies
to develop ELISA and Western blotting assays to detect NPVs. The sensitivity of
the ELISA was 15 ng/ml of semi-purified viral protein or 30 ng/ml POBs from the
NPV infected larval extracts. These antibodies are useful to diagnose the early
stages of larval infection by NPV and also for the quantification of the NPVs during
production of viral insecticides for HaNPV, SlNPV, and AmalNPV (Sridhar Kumar
et al. 2007). For qualitative detection of NPVs in larval homogenates, Western
immunoblotting and indirect immunofluorescence assay, and for quantitative detec-
tion direct antigen coating (DAC) and indirect competitive (IC)-ELISA tools were
developed and evaluated (Sridhar Kumar 2008). Since, polyhedrin is the major
component of NPV polyhedra, is coded by the virus, and its presence in larvae
indicates the presence of NPV or an NPV infection. Similarly, the relationship
between three NPVs isolated from the larvae of H. armigera, S. exigua, and S. litura
in Taiwan was determined by assaying the polyhedrin in DAC-ELISA with
polyhedrin polyclonal antisera specific to each polyhedrin (Tuan et al. 1999), and
similarly, a monoclonal antibody-based DAC-ELISA was developed for the iden-
tification and differentiation of OpMNPV and OpSNPV and also for detection of
their homologous polyhedrin in larval extracts with sensitivity of 100 ng/ml in the
presence of host tissue extract, rather than 5 ng/ml in its absence (Quant et al. 1984).
However, competition between insect and viral proteins for binding to ELISA plate
surface has reduced the detection sensitivity of the DAC-ELISA, particularly when
crude insect extracts were used. Since it was found that host tissue extract interfered
with the assay, still we were able to determine its absolute sensitivity in the
presence of unknown quantities of host tissue extract. To eliminate the competition
between insect and viral proteins for binding sites in the ELISA plate surface in
DAC-ELISA, we attempted to avoid the interference phenomenon by standardizing
an IC-ELISA to estimate the polyhedrin content in insect extracts. Similarly, an
IC-ELISA was standardized to evaluate the bio-safety of recombinant and wild-
type NPV of A. californica (Ashour et al. 2007). Similarly, Crook and Payne (1980)
examined the direct, indirect, and double antibody sandwich methods of ELISA for
their ability to detect and discriminate between granulosis viruses from Pieris
brassicae, Agrotis segetum, and C. pomonella and for their specificity in the
presence of host material, and they concluded that the indirect method was the
most sensitive and capable of detecting down to about 1 ng of dissolved capsules/ml
compared with 10 ng/ml for the double antibody sandwich method and 25 ng/ml for
the direct method and the double antibody sandwich method was more specific and
showed greatest discrimination between different granulosis viruses.
Also the DAC and IC-ELISA tools were evaluated for their performance in
quantification of POBs in commercial NPV preparations. The number of POBs
present in the sample bottle was determined by extracting the total polyhedrin and
compared with the standard regression graph of polyhedrin extracted from known
number (estimated by microscopic counting) of POB standards such as 6 109 to
2.34 107 POBs/ml (1 LE to 0.0078 LE). These ELISA methods are sensitive to a
minimum of approximately 4.6875 107 POBs/ml (0.015 LE/ml), which is little bit
higher to the range (100–2,000) of previous reports (Crook and Payne 1980; Kelly
et al. 1978; Longworth and Carey 1980; Shamim et al. 1994). The ELISA methods
can be used to quantify infection, unlike other methods, and this feature may be
applied to predict the potential inoculum required for future populations. Previ-
ously, it has been shown that the ELISA method can be used to quantify
baculoviruses (Clark and Barbara 1987). Tuan et al. (1998) compared the visual
counting of POBs by microscope, bioassay, SDS-PAGE, and ELISA for quality
control of SlNPV products, and ELISA has proved to be better than SDS-PAGE.
The ELISA results were comparable to light microscope counting of POBs (Sridhar
Kumar 2008). The absorbance values suggest that the ELISA method can be used to
accurately quantify virus POBs and virus infections from tissue homogenates.
Recently, Thorne et al. (2007) reported that the alkali-treated POB lysates were
assayed in IC-ELISA for estimation of total POBs in semi-purified commercial
NPV samples or in insect tissue extracts with a sensitivity of minimum of approx-
imately 850 POBs. Similarly, Parola et al. (2003) reported the estimation of
granulin in commercial GV suspensions of Epinotia aporema GV by
DAS-ELISA with a sensitivity of 0.53 ng/ml of purified OB suspensions; this
represented 2.0 104 OBs/ml.
These diagnostic and quality control tests are convenient for routine detection
and quantification of NPVs, and this technology will also be transferred to the
bio-products agribusiness units for commercialization of NPV production. Also the
ELISA and Western immunoblot assays can be successfully applied in bioassay
experiments during optimization of conditions for the productivity and quality of
NPVs to get the maximum virus yield as well as to reduce the development of
bacterial contamination. In addition to this application of ELISA tools at field level,
evaluation of the efficacy of NPVs is useful for ecological and epidemiological
studies of NPVs during IPM programs and also during the surveys of their persis-
tence and outbreaks of natural epizootics in the environment.
2.7 Way Forward
• Over the past 25 years, the research approach on NPVs has evolved toward being
more ecologically holistic with industry’s concerns.
• Although viral pesticides still represent a very small portion of plant protection
at present, their role was considered significant.
• Though NPVs gained prominence as environmentally friendly alternatives to
chemical insecticides, they still face a number of hurdles in their production,
marketing, and utilization.
• Importance of effective multidisciplinary research, public, private, people
partnerships.
• Need for in-depth knowledge among farmers, extension, and policy makers
about bio-pesticides.
• Lack of effective regulations can lead to poor product quality, performance, and
loss of user confidence.
• NPVs that can perform effectively in wider environments and larger host range
have immense potential.
• Prioritize research for better integration of bio-agents into production systems,
such as in rotating these with chemical pesticides and developing these into
effective bio-models.
References
Abul-Nasr S, Elnagar S (1980) The susceptibility of the cotton leafworm, Spodoptera littoralis
(Boisd) to different isolates of nuclear polyhedrosis virus. J Appl Entomol 90:289–292
Aizawa K (1963) The nature of infections caused by nuclear polyhedrosis viruses. In: Steinhaus
EA (ed) Insect pathology: an advanced treatise, vol 1. Academic, New York, pp 381–412
Anderson RF, Shieh TR, Huang HT, Rogoff MH (1972) Production of a viral pesticide. Proc Symp
Fermentat Technol Today 2:493–505
Angelini A, Labonne V (1970) A technique for the rearing of Heliothis armigera (Hub.) and a
possibility of producing a nuclear virus disease in the Ivory Coast. Cotton Fibres Tropicales
25:501–504
Anon (2009) Biopesticides market to reach $ 1 billion in 2010. http://www.ien.com/article/
biopesticides-market-to/8648
Arguad O, Croizier L, Lopez-Ferber M, Croizier G (1998) Two key mutations in the host-range
specificity domain of the p143 gene of Autographa californica nucleopolyhedrovirus are
required to kill Bombyx mori larvae. J Gen Virol 79:931–935
Armes NJ, Jadhav DR, Bond GS, King ABS (1992) Insecticide resistance in Helicoverpa armigera
in South India. Pestic Sci 34:355–364
Arora R, Battu GS, Bath DS (1997) Comparative evaluation of some native isolates of a nuclear
polyhedrosis virus against Heliothis armigera (Hübner). J Entomol Res 21:183–186
Ashour MB, Ragheb DA, EI-Sheikh EI-Sayed A, Gomaa EI-Adarosy A, Kamita Shizuo G,
Hammock Bruce D (2007) Biosafety of recombinant and wild type nucleopolyhedrovirus as
bioinsecticides. Environ Res Public Health 4:111–125
Attathom T, Chaeychomsri S, Mahattana C, Siriwattanagul W (1990) Technological development
for the local production of nuclear virus of Helicoverpa armigera. In: Proceedings on V
international colloquium on invertebrate pathology and microbial control, Adelaide, 20–
24 August 1990
Backwad DG (1979) Studies on NPV infection in Heliothis armigera (Hubner) and Anomis
sabulifera. M.Sc. (Ag.) Thesis, Marathwada Agriculture University, Parbhani, p 141
Banowetz GM, Fryer JL, Iwai PJ, Martignoni ME (1976) Effects of the douglas-fir tussock moth
nucleopolyhedrosis virus (baculovirus) on three species of salmonid fish. Forest Service
Research Paper PNW-214. U.S. Department of Agriculture, Washington, DC
Bansal OB, Bansal A, Behera AK, Das RH, Kumar M (1997) Characterization of the polyhedron
gene of Spodoptera litura nuclear polyhedrosis virus. Virus Genes 14:175–180
Battu GS (1990) Yield levels of the occlusion bodies obtained from the Baculovirus infected
Hellula undalis (Fabricius). Ind J Entomol 51:317–325
Battu GS (1992) Potential use of baculoviruses on Lepidopteran pests in Punjab. In:
Ananthakrishnan TN (ed) Emerging trends in biological control of phytophagous insects.
Oxford and IBH Company Private Ltd., New Delhi, pp 174–181
Battu GS, Ramakrishna N (1987) Investigations on the nuclear polyhedrosis virus of Diacrisia
oblique (Walker): bioassay of viral activity. Entomon 12:81–84
Bell MR (1991) Effectiveness of microbial control of Heliothis spp. on early season wild
geraniums. Field and field cage tests. J Econ Entomol 84:851–854
Bellotti AC (1999) Recent advances in cassava pest management. Annu Rev Entomol 44:345–370
Benz GA (1986) Introduction: historical perspective. In: Granados RR, Federici BA (eds) The
biology of baculoviruses. CRC, Boca Raton, pp 1–35
Bergold GH (1947) Die Isolierung des Polyeder Virus und die Natur der Polyeder. Z Naturforsch
2B:122–143
Bergold GH (1948) Uber die Kapsel virus-krankheit. Z Naturforsch 3B:338–342
Bergold GH (1982) The nature of the nuclear polyhedrosis viruses. Biol Baculoviruses 413–453
Biji CP, Sudheendrakumar VV, Sajeev TV (2006) Influence of virus inoculation method and host
larval age on productivity of the nucleopolyhedrovirus of the teak defoliator, Hyblaea puera
(Cramer). J Virol Methods 133:100–104
Blissard GW, Rohrmann GF (1990) Baculovirus diversity and molecular biology. Annu Rev
Entomol 35:127–155
Blissard GW, Black B, Crook N, Keddie BA et al (2000) Family Baculoviridae. In: Van
Regenmortel MHV et al (eds) Virus taxonomy: seventh report of the International Committee
on Taxonomy of Viruses. Academic, San Diego, pp 195–202
Braunagel SC, Russell WK, Rosas Acosta G, Russell DH, Summers MD (2003) Determination of
protein composition of the occlusion-derived virus of Autographa californica nucleopolyhe-
drovirus. Proc Natl Acad Sci USA 100:9797–9802
Brown SE, Maruniak JE, Knudson DL (1984) Physical map of SeMNPV baculovirus DNA: an
AcMNPV genomic variant. Virology 136:235–240
Bulach DM, Kumar CA, Zaia A, Liang BF, Tribe DE (1999) Group-II nucleopolyhedrovirus
subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene
sequences. J Invertebr Pathol 73:59–73
Burges HD (1981) Microbial control of pests and plant diseases, 1970–1980. Academic,
New York, p 949
Burges HD, Thompson EM (1971) Standardisation and assay of microbial insecticides. In: Burges
HD, Hussey NW (eds) Microbial control of insects and mites. Academic, London, pp 591–622
Caballero P, Aldebis HK, Vargas-Osuna E, Santiago-Alvarez C (1992) Epizootics caused by a
nuclear polyhedrosis virus (NPV) in populations of Spodoptera exigua in Southern Spain. Bio
Sci Technol 2:45–149
Carter JB (1984) Viruses as pest control agents. Biotechnol Gen Eng Rev 1:375–405
Chakraborthy S, Monsour C, Teakle R, Reid S (1999) Yield, biological activity, and field
performance of a wild-type Helicoverpa Nucleopolyhedrovirus produced in H. zea cell cul-
tures. J Invertebr Pathol 73:199–205
Chakraborty S, Green Field P, Reid S (1996) In-vitro production studies with a wild type
Helicoverpa baculovirus. Cytotechnology 22:217–224
Christian PD, Gibb N, Kasprzak AB, Richards A (2001) A rapid method for the identification and
differentiation of Helicoverpa nucleopolyhedroviruses (NPV Baculoviridae) isolated from the
environment. J Virol Methods 96:51–65
Clark M, Barbara D (1987) A method for quantitative analysis of ELISA data. J Virol Methods
15:213–222
Claus Juan D, Gioria Ver onica V, Micheloud Gabriela A et al (2012) Production of insecticidal
baculoviruses in insect cell cultures: potential and limitations. In: Soloneski S (ed) Insecticides
– basic and other applications. In Tech. Available from: http://www.intechopen.com/books/
insecticides
Croizier G, Ribeiro HCP (1992) Recombination as a possible major cause of genetic heterogeneity
in Anticarsia gemmatalis nuclear polyhedrosis virus populations. Virus Res 26:183–196
Crook NE, Payne CC (1980) Comparison of three methods of ELISA for baculoviruses. J
GenVirol 46:29–37
David WAL, Gardiner BOC (1966) Persistence of a granulosis virus of Pieris brassicae on
cabbage leaves. J Invertebr Pathol 8:180–183
David WAI, Gardiner BOC (1967a) The effect of heat, cold, and prolonged storage on a granulosis
virus of Pieris brassicae. J Invertebr Pathol 9:555–562
David WAL, Gardiner BOC (1967b) The persistence of a granulosis virus of Pieris brassicae in
soil and in sand. J Invertebr Pathol 9:342–347
David WAL, Ellaby SV, Taylor G (1971) The stability of a purified granulosis virus of the
European cabbageworm, Pieris brassicae, in dry deposits of intact capsules. J Invertebr Pathol
17:228–233
De Moraes RR, Maruniak JE (1997) Detection and identification of multiple baculoviruses using
the polymerase chain reaction (PCR) and restriction endonuclease analysis. J Virol Methods
63:209–217
Doyle CJ, Hirst ML, Cory JS, Entwistle PF (1990) Risk assessment studies: detailed host range
testing of wild-type cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae) nuclear
polyhedrosis virus. Appl Environ Microbiol 56:2704–2710
Ebora RV, Shepard BM, Cadapan EP (1990) Mass propagation and factors affecting virulence of a
nuclear polyhedrosis virus of Spodoptera litura (Fab.). Philippine J Biotechnol 2:138–148
Falcon LA (1971) Microbial control as a tool in integrated control programs. In: Huffaker CB
(ed) Biological control. Plenum, New York, pp 346–364
Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) (2004) Virus taxonomy. VII
report of the ICTV. Elsevier/Academic, London, p 1258
Federici BA (1978) Baculovirus epizootic in a larval population of the clover cutworm,
Scotogramma trifolii, in Southern California. Environ Entomol 7:423–427
Federici BA (1997) Baculovirus Pathogenesis. In: Miller LK (ed) The baculoviruses. Plenum,
New York, pp 33–59
Federici BA, Hice RH (1997) Organization and molecular characterization of genes in the
polyhedrin region of the Anagrapha falcifera multinucleocapsid NPV. Arch Virol 142:333–
348
Figueiredo E, Munoz D, Escribano A, Mexia A, Vlak J, Caballero P (1999) Biochemical identi-
fication and comparative insecticidal activity of nucleopolyhedrovirus isolates pathogenic for
Heliothis armigera larvae. J Appl Entomol 123:1–00
Funk CJ, Braunagel SC, Rohrmann GF (1997) Baculovirus structure. In: Miller LK (ed) The
baculoviruses. Plenum, New York, pp 7–32
Geetha N, Rabindra RJ (1999) Genetic variability and comparative virulence of some geographic
isolates of nuclear polyhedrosis virus of Helicoverpa armigera Hub. In: Ignacimuthu S, Sen A,
Janarthana S (eds) Biotechnological application for integrated pest management. Oxford and
IBH Publ. Co. Pvt. Ltd., New Delhi, pp 65–78
Gelernter WD, Federici BA (1990) Virus epizootics in California populations of Spodoptera
exigua: dominance of a single genotype. Biochem Syst Ecol 18:461–466
Gopali JB, Lingappa S (2001a) Refinement of technique for mass production of virus from
Helicoverpa armigera (Hubner). Karnataka J Agric Sci 14:947–954
Gopali JB, Lingappa S (2001b) Evaluation of safety period for field use of virus (HaNPV) under
different set of storage conditions. Karnataka J Agric Sci 14:1072–1074
Grace TD (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature
195:788–789
Granados RR, McKenna KA (1995) Insect cell culture methods and their use in virus research. In:
Wood HA, Granados RR, Schuler ML et al (eds) Baculovirus expression systems and
biopesticides. Wiley-Liss, New York, pp 13–39
Groner A (1986) Specificity and safety of baculoviruses. In: Granados RR, Federici BA (eds) The
biology of baculoviruses, biological properties and molecular biology, vol I. CRC, Boca Raton,
pp 177–202
Grzywacz D, McKinley D, Jones KA et al (1997) Microbial contamination in Spodoptera littoralis
nuclear polyhedrosis virus produced in insects in Egypt. J Invertebr Pathol 69:151–156
Gudauskas RT, Cannerday D (1968) The effect of heat, buffer salt and H-ion concentration, and
ultraviolet light on the infectivity of Heliothis and Trichoplusia nuclear polyhedrosis viruses. J
Invertebr Pathol 12:405–411
Guo ZJ, Ge JQ, Wang D, Shao YM, Tang QY, Zang CX (2006) Biological comparison of two
genotypes of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus. BioControl.
doi:10.1007/s1056-006-9008-6
Gupta RK, Raina JC, Monobrullah MD (2007) Optimization of In vivo production of nucleopo-
lyhedrovirus in homologous host larvae of Helicoverpa armigera. J Entomol 4:279–288
Gupta RK, Raina JC, Arora RK, Bal K (2010) Selection and field effectiveness of nucleopolyhe-
drovirus isolates against Helicoverpa armigera (Hubner). Int J Virol 6:164–178
Harrap KA, Payne CC, Robertson JS (1977) The properties of three baculoviruses from closely
related hosts. Virology 79:14–31
Harris JG (1997) Microbial insecticides, an industry perspective in microbial insecticides: novelty
or necessity? Br Crop Protect Council Proc Monograph Ser 68:41–50
Harrison RL, Bonning BC (1999) The nucleopolyhedroviruses of Rachiplusia ou and Anagrapha
falcifera are isolates of the same virus. J Gen Virol 80:2793–2798
Hedlund RC, Yendol WG (1974) Gypsy moth nuclear polyhedrosis virus production as related to
inoculating time, dosage and larval weight. J Econ Entomol 67:61–63
Herniou EA, Olszewski JA, Cory JS, O’ Reilly DR (2003) The genome sequence and evolution of
baculoviruses. Annu Rev Entomol 48:211–234
Hink WF (1982) Production of Autographa californica nuclear polyhedrosis virus in cells from
large scale suspension cultures. In: Kurstak E (ed) Microbial and viral pesticides. Marcel
Dekker, New York, pp 493–506
Hooft van Iddekinge BJK, Smith GE, Summers MD (1983) Nucleotide sequence of polyhedrin
gene of Autographa californica nuclear polyhedrosis virus. Virology 131:5561–5565
Hostetter DL, Puttler B (1991) A new broad host spectrum nuclear polyhedrosis virus isolated
from a celery looper, Anagrapha falcifera (Kirby), (Lepidoptera: Noctuidae). Environ Entomol
20:1480–1488
Huber J (1985) Progress in the production of codling moth granulosis virus: comparison of several
virus preparations. In: Production and application of biopesticides on a viral basis in orchards
and vegetables. Expert meeting, Manfauet, 5–7 July 1985. Commission of European commu-
nities, General Directorate Agriculture
Huber J, Krieg A (1978) Studies with baculoviruses on mammals. Z Angew Zool 65:69–80
Hughes PR, Getting RR, McCarthy WJ (1983) Comparison of the time mortality response of
Heliothis zea to 14 isolates of Heliothis nuclear polyhedrosis virus. J Invertebr Pathol 41:256–
261
Hunter DK, Collier SJ, Hoffman DF (1975) Compatibility of malathion and the granulosis virus of
the Indian meal moth. J Invertebr Pathol 25:389–390
Hunter-Fujita RF, Entwistle PF, Evans FH, Crook NE (1998) Insect virus and pest management.
Wiley, Chichester, p 620
Ignoffo CM (1965) The nuclear polyhedrosis virus of Heliothis zea (Boddie) and Heliothis
virescens (Fabricius) I-virus propagation and its virulence. J Invertebr Pathol 7:209–216
Ignoffo CM (1966a) Insect viruses. In: Smith CN (ed) Insect colonization and mass production.
Academic, New York, pp 501–530
Ignoffo CM (1966b) Standardization of products containing insect viruses. J Invertebr Pathol
48:547–548
Ignoffo CM (1973) Effects of entomopathogens on vertebrates. Ann NY Acad Sci 217:141–172
Ignoffo CM, Boeing OP (1970) Compartmental disposable trays for rearing insects. J Econ
Entomol 63:1696–1697
Ignoffo CM, Couch TL (1981) The nuclear polyhedrosis virus of Heliothis species as a microbial
insecticide. In: Burges HD (ed) Microbial control of pests and plant diseases 1970–1980.
Academic, London pp, pp 329–362
Ignoffo CM, Rafajko RR (1972) In vitro attempts to infect primate cells with the nucleopoly-
hedrosis virus of Heliothis. J Invertebr Pathol 20:321–325
Ignoffo CM, Shapiro M (1978) Characteristics of baculovirus preparations processed from living
and dead larvae. J Econ Entomol 71:186–188
Injac M (1973) The role of granulosis in regulating the density of population of fall webworm
(Hyphantria cunea Drury) in Vojvodina, Yugoslavia. Zast Bilja 24:103–110
Jacques RP, Long DR (1978) Efficacy of mixture of Bacillus thuringiensis, viruses and
chlordimeform against insects on cabbage. Can Entomol 110:443–448
Jayaraj S, Sathiah N (1993) Heliothis culture techniques. In: Proceedings of national training on
mass multiplication of biocontrol agents, Tamil Nadu Agricultural University, Coimbatore, pp
1–4
Jehle JA, Fritsch E, Nickel A, Huber J, Backhaus H (1995) TC14.7: a novel Lepidopteran
transposon found in Cydia pomonella granulosis virus. Virology 207:369–379
Jun T, Shohei O, Takayoshi I, Madoka N, Yasuhisa K (2007) Productivity and quality of
polyhedral occlusion bodies of a nucleopolyhedrovirus harvested from Spodoptera litura
(Lepidoptera: Noctuidae) larvae. Appl Entomol Zool 42:21–26
Kambrekar DN, Kulkarni KA, Giraddi RS (2007) Assessment of quality of HaNPV samples
produced by private firms. Karnataka J Agric Sci 20:417–419
Kamita SG, Maeda S (1997) Sequencing of the putative DNA helicase-encoding gene of the
Bombyx mori nuclear polyhedrosis virus. Gene 190(1):173–9
Katagiri K (1981) Pest control by cytoplasmic polyhedrosis viruses. In: Burges HD (ed) Microbial
control of pests and plant diseases 1970–1980. Academic, New York, pp 433–440
Kelly DC, Edwards ML, Evans HF, Robertson JS (1978) The use of the enzyme linked immuno-
sorbent assay to detect a nuclear polyhedrosis virus in Heliothis armigera larvae. J Gen Virol
40:465–469
Kelly DC, Brown DA, Robertson JS, Harrap KA (1980) Biochemical, biophysical and serological
properties of two singly enveloped nuclear polyhedrosis viruses from H. armigera and H. zea.
Microbiologica 3:319–331
Kern M, Vaagt G (1996) Pesticide quality in developing countries. Pesticide Out Look 7–10
Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibodies of predefined
specificity. Nature 256:495–497
Kool M, Vonken JW, Van Lier FLJ, Tramper J, Vlak JM (1991) Detection and analysis of
Autographa californica nuclear polyhedrosis virus mutants with defective interfering proper-
ties. Virology 183:739–746
Kranthi KR, Jadhav DR, Kranthi S, Wanjari RR, Ali SS, Russel DA (2002) Insecticide resistance
in five major insect pests of cotton in India. Crop Prot 21:449–460
Krell PJ (1996) Passage effect of virus interaction in insect cells. In: Vlak JM, De Gooigee CD,
Tramper J et al (eds) Insect cell culture, fundamentals and applied aspects. Kluwer Academic,
A.H. Dordrecht, pp 125–137
Lange M, Wang H, Hu Z, Jehle JA (2004) Towards a molecular identification and classification
system of lepidopteran-specific baculoviruses. Virology 325:36–47
Lautenschlager RA, Kircher CH, Podgwaite JD (1977) Effect of nuclear polyhedrosis virus on
selected mammalian predators of the gypsy moth. Forest Service Research Paper NE-377.
U.S. Department of Agriculture, Washington, pp 9–29
Lee HH, Miller LK (1978) Isolation of genotypic variants of Autographa californica nuclear
polyhedrosis virus. J Virol 27:754–767
Linda HL, Lua Steven Reid LS (2003) Growth, viral production and metabolism of a Helicoverpa
zea cell line in serum-free culture. Cytotechnology 42:109–120
Lisansky S (1997) Microbial biopesticides, in microbial insecticides Novelty or Necessity? Br
Crop Protect Council Proc Monograph Ser 68:3–10
Longworth JF, Carey GP (1980) The use of an indirect enzyme-linked immunosorbent assay to
detect baculovirus in larvae and adults of Oryctes rhinoceros from Tonga. J Gen Virol 47:431–
438
Lu ZQ, Hu GG, Chen LF, Yang F, Hang SB, Le YX, Su DM (1995) Use of monoclonal antibodies
and ELISA to detect nuclear polyhedrosis virus (NPV) in the larvae of Helicoverpa armigera.
Chinese J Biol Control 11:36–39
Lua HL, Reid S (2000) Serial passage of a Helicoverpa armigera nucleopolyhedrosis virus in
Helicoverpa zea serum free suspension culture. J Gen Virol 81:2531–2543
Lynn DE (1999) Development of insect cell lines: virus susceptibility and applicability to prawn
cell culture. Methods Cell Sci 21:173–181
Ma XC, Xu HJ, Tang MJ, Xiao Q, Hong Q, Zhang CX (2006) Morphological, phylogenetic and
biological characteristics of Ectropis oblique single-nucleocapsid nucleopolyhedrovirus. J
Microbiol 44:77–82
Maeda S, Mukohara Y, Kondo A (1990) Characteristically distinct isolates of the nuclear poly-
hedrosis virus from S. litura. J Gen Virol 71:2631–2639
Maskos CB, Miltenburger HG (1981) SDS-PAGE comparative studies on the polyhedral and viral
polypeptides of the nuclear polyhedrosis viruses of Mamestra brassicae, Autographa
californica, and Lymantria-dispar. J Invertebr Pathol 37:174–180
Matthiessen JN, Christian RL, Grace TDC et al (1978) Large scale field propagation and purifi-
cation of the granulosis virus of potato moth, Phthorimaea operculella (Lep: Gel). Bull
Entomol Res 68:385–391
Miller LK, Dawes KP (1978) Restriction endonuclease analysis to distinguish two closely related
nuclear polyhedrosis viruses: Autographa californica MNPV and Trichoplusia ni MNPV. Appl
Environ Microbiol 35:1206–1210
Mitsuhashi J (1989) Nutritional requirements of insect cells in vitro. In: Mitsuhashi J
(ed) Invertebrate cell system applications. CRC, Boca Raton, pp 3–21, ISBN 0849343739
Monobrullah M (2003) Optical brighteners – pathogenicity enhancers of entomopathogenic
viruses. Curr Sci 84:640–645
Monobrullah M, Nagata M (1999) Immunity of lepidopteran insects against baculoviruses. J
Entomol Res 23:185–194
Monroe JE, McCarthy WJ (1984) Polypeptide analysis of genotypic variants of occluded Heliothis
spp. Baculoviruses. J Invertebr Pathol 43:32–40
Moscardi F (1999) Assessment of the application of baculoviruses for control of Lepidoptera.
Annu Rev Entomol 44:257–290
Moscardi F, Allen GE, Greene GL (1981) Control of the velvetbean caterpillar by nuclear
polyhedrosis virus and insecticides and impact of treatments on the natural incidence of the
entomopathogenic fungus Nomuraea rileyi. J Econ Entomol 74:48–485
Munoz D, Castillejo JI, Caballero P (1998) Two naturally occurring deletion mutants are parasitic
genotypes in a Spodoptera exigua nucleopolyhedrovirus strain. Appl Environ Microbiol
64:4372–4377
Murillo R, Munoz D, Lipa JJ, Caballero P (2001) Biochemical characterization of three nucleopo-
lyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae. J Appl Entomol 125:267–
270
Nakai M, Goto C, Kang W, Shikata M, Luque T, Kunimi Y (2003) Genome sequence and
organization of a nucleopolyhedrovirus isolated from the smaller tea tortrix, Adoxophyes
honmai. Virology 316:171–183
Narayanan K (1979) Studies on the nuclear polyhedrosis virus of gram pod borer, Heliothis
armigera (Hubner) (Noctuidae: Lepidoptera). Ph.D. Thesis, Tamil Nadu Agriculture Univer-
sity, Coimbatore, p 204
Narayanan K (1985) Isolation, purification and inoculation of viral pathogens. In: Jayaraj S
(ed) Microbial control and pest management. Tamil Nadu Agriculture University, Arul Jothi
Printers, Coimbatore, pp 55–59
Nguyen Q, Qi YM, Wu YC et al (2011) In vitro production of Helicoverpa baculovirus
biopesticides—automated selection of insect cell clones for manufacturing and systems biol-
ogy studies. J Virol Methods 175:197–205
Odak SK, Srivastava DK, Misra VK et al (1984) Preliminary studies on the pathogenicity of
Bacillus thuringiensis and nuclear polyhedrosis virus on Heliothis armigera host in the
laboratory and in pot experiments. Legume Res 5:13–17
Ogemo JG, Chaeychomsri S, Kamiya K et al (2007) Cloning and comparative characterization of
nucleopolyhedrosis viruses isolated from African bollworm, Helicoverpa armigera (Lepidop-
tera: Noctuidae) in different geographic regions. J Insect Biotechnol Sericol 76:39–49
Pant U, Sudeep AB, Athawale SS, Vipat VC (2002) Baculovirus studies in new, indigenous
lepidopteran cell lines. Indian J Exp Biol 40:63–68
Parola AD, Cap AS, Glikmann G, Romanowski V (2003) An immunochemical method for
quantification of Epinotia aporema granulovirus (EpapGV). J Virol Methods 112:13–21
Pawar VM, Thombre UT (1992) Prospects of baculovirus in integrated pest management of pulses.
In: Ananthkrishnan TN (ed) Emerging trends in biocontrol of phytophagous insects. Oxford
and IBH Publishing Company Private Limited, New Delhi, pp 253–258
Podgwaite JD, Bruen RB, Shapiro M (1983) Microorganisms associated with production lots of
the nuclear polyhedrosis virus of the gypsy moth, Lymantria dispar. Entomophaga 28:9–16
Possee RD, Rohrmann GF (1997) Baculovirus genome organization and evolution. In: Miller LK
(ed) The baculoviruses. Plenum, New York, pp 109–133
Powell JE, Robertson JL (1993) Status of rearing technology for cotton insects. In: King EG,
Brown JM (eds) cotton insects and mites: characterization and management. Cotton Founda-
tion, Memphis
Quant RL, Pearson MN, Rohrman GF et al (1984) Production of polyhedrin monoclonal anti-
bodies for distinguishing two Orgyia pseudotsugata baculoviruses. Appl Environ Microbiol
48:732–736
Rabindra RJ (1992) Genetic improvement of baculoviruses. In: Ananthkrishnan TN (ed) Emerging
Trends in Biological control of phytophagous insects. Oxford and F B H Publishing Cooper-
ative Private Ltd., New Delhi, pp 183–186
Rabindra RJ, Subramanian TR (1974) Studies on the nuclear polyhedrosis of Heliothis armigera
(Hubner). Madras Agric J 60:217–220
Rao GVR (2006) SATrends 64 ICRISAT Publication
Rao GVR, Meher KS (2004) Optimization of In vivo production of Helicoverpa armigera NPV
and regulation of malodor associated with the process. Indian J Plant Protect 32:15–18
Rao GVR, Rupela OP, Rameshwar Rao V, Reddy YVR (2007) Role of biopesticides in crop
protection: present status and future prospects. Indian J Plant Protect 35:1–9
Reardon R, Podgwaite JP, Zerillo RT (1996) GYPCHECK – the gypsy moth nucleopolyhedrosis
virus product. USDA Forest Service Publication FHTET-96-16
Rivkin H, Mor M, Chejanovsky N (1998) Isolation, replication and polyhedrin gene sequence of
an Israeli Helicoverpa armigera single nucleopolyhedrovirus. Virus Genes 17:11–19
Roder A, Punter J (1977) Interactions between nuclear polyhedrosis viruses and vertebrate cells.
Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt 1 Orig Reihe A 239:459–464
Rohrmann GF (1986) Evolution of occluded baculoviruses. In: Granados R, Federici B (eds) The
biology of baculoviruses, vol 1. CRC, Boca Raton, pp 203–215
Rohrmann GF (1992) Baculovirus structural proteins. J Gen Virol 73:749–761
Rohrmann GF (1999) Nuclear polyhedrosis viruses. In: Webster RG, Granoff A (eds) Encyclo-
pedia of virology, 2nd edn. Academic, London, pp 146–152
Rollinson WD, Hubbard HB, Lewis FB (1970) Mass rearing of the European pine sawfly for
production of the nuclear polyhedrosis virus. J Econ Entomol 63:343–344
Rovesti L, Crook NE, Winstanely D (2000) Biological and biochemical relationships between the
nucleopolyhedroviruses of Mamestra brassicae and Heliothis armigera. J Invertebr Pathol
75:2–8
Russell RLQ, Rohrmann GF (1990) A baculovirus polyhedron envelope protein: immuno gold
localization in infected cells and mature polyhedra. Virology 174:177–184
Saxene H, Ahmad R (2005) NPV production, formulation and quality control. In: Saxena H, Rai
AB, Ahmed R et al (eds) Recent advances in Helicoverpa management. Proceedings of the
national symposium on Helicoverpa management: a national challenge, IIPR, pp 1–10
Senthil Kumar CM, Sathiah N, Rabindra RJ (2005) Optimizing the time of harvest of nucleopo-
lyhedrovirus infected Spodoptera litura (Fabricius) larvae under In vivo production systems.
Curr Sci 88:1682–1684
Shamim M, Baig M, Datta RK, Gupta SK (1994) Development of monoclonal antibody-based
sandwich ELISA for the detection of nuclear polyhedra of nuclear polyhedrosis virus infection
in Bombyx mori L. J Invertebr Pathol 63:151–156
Shapiro M, Bell RA (1981) Biological activity of Lymantria dispar nucleopolyhedrosis virus from
living and virus killed larvae. Ann Entomol Soc Am 74:27–28
Shapiro M, Bell RA (1982) Production of gypsy moth, Lymantria dispar (L.) nucleopolyhedrosis
virus, using carrageenans as dietary gelling agents. Ann Entomol Soc Am 75:43–45
Shapiro M, Ignoffo CM (1969) Nuclear polyhedrosis of Heliothis: stability and relative infectivity
of virions. J Invertebr Pathol 14:130–134
Shapiro M, Ignoffo CM (1970) Nucleopolyhedrosis of Heliothis: activity of isolates from
Heliothis Zea. J Invertebr Pathol 16:107–111
Shapiro M, Robertson JL, Injac MG, Katagiri K, Bell RA (1984) Comparative infectivities of
gypsy moth (Lepidoptera: Lymantriidae) nucleopolyhedrosis virus isolates from North Amer-
ica, Europe and Asia. J Econ Entomol 77:153–156
Shapiro M, Robertson JL, Bell RA (1986) Quantitative and qualitative differences in gypsy moth
(Lep: Lyman) NPV produced in different – aged larvae. J Econ Entomol 79:1174–1177
Sherman KE (1985) Considerations in the large scale and commercial production of viral
insecticides. In: Karl M, Sherman KE (eds) Viral insecticides for biological control. Academic,
London, pp 757–797
Shieh TR (1978) Characteristics of a viral pesticide, Elcar. In: Proceedings of international
colloquium and invertebrate pathology, pp 91–194
Shieh TR (1989) Industrial production of viral pesticides. Adv Virus Res 36:315–343
Shieh TR, Bohmfalk GT (1980) Production and efficiency of baculoviruses. Biotechnol Bioeng
22:1357–1375
Shim HJ, Roh JY, Choi JY, Li MS, Woo SD, Oh HW, Boo KS, Je Y (2003) Isolation and
characterization of a Lymantria dispar multinucleocapsid nucleopolyhedrovirus isolate in
Korea. J Microbiol 41:306–311
Sireesha K (2006) Determination of efficacy of different Helicoverpa armigera nucleopolyhe-
drovirus (HaNPV) strains and standardization of production procedures. Ph.D. Thesis, Depart-
ment of Entomology, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural
University, Rajendranagar, Hyderabad
Smith IRL, Crook NE (1988) In vivo isolation of baculovirus genotypes. Virology 166:240–244
Smith GE, Summers MD (1978) Analysis of baculovirus genomes with restriction endonucleases.
Virology 89:517–527
Smith GE, Summers MD (1979) Restriction maps of five Autographa californica MNPV variants,
Trichoplusia ni MNPV, and Galleria mellonella MNPV DNAs with endonucleases SmaI,
KpnI, BamHI, SacI and EcoRI. J Virol 30:393–406
Smith GE, Summers MD (1981) Application of a novel radioimmunoassay to identify baculovirus
structural proteins that share interspecies antigenic determinants. J Virol 39:125–137
Smith GE, Summers MD (1982) DNA homology among subgroup A, B and C baculoviruses.
Virology 123:393–406
Smits PH, Vlak JM (1987) Biological activity of Spodoptera exigua nuclear polyhedrosis virus
against S. Exigua larvae. J Invertebr Pathol 51:107–114
Somasekhar S, Jayapragasam M, Rabindra RJ (1993) Characterization of five Indian isolates of the
nuclear polyhedrosis virus of H. armigera. Phytoparasitica 21:333–337
Sridhar Kumar Ch (2008) Development and evaluation of diagnostic tools for nucleopolyhe-
droviruses infecting major lepidopteran pests of legume crops in the semi-arid tropics. Ph.D.
Thesis, Department of Biochemistry, Acharya Nagarjuna University, Andhra Pradesh
Sridhar Kumar C, Sireesha K, Ranga Rao GV, Reddy AS, Waliyar F, Rambabu C, Kumar L (2007)
Production of polyclonal antibodies for detection of Nucleopolyhedrovirus infecting
Helicoverpa armigera. Indian J Plant Protect 35:210–212
Sridhar Kumar C, Ranga Rao GV, Sireesha K, Kumar PL (2011) Isolation and characterization of
baculoviruses from three major lepidopteran pests in the semi-arid tropics of India. Indian J
Virol 22:29–36
Stairs GR, Frasor T, Frasor M (1981) Changes in growth and virulence of a nuclear polyhedrosis
virus from Choristoneura fumiferana after passage in Trichoplusia ni and Galleria mellonella.
J Invertebr Pathol 38:230–235
Sudhakar S, Mathavan S (1999) Viral pesticides for environmental safety (Helicoverpa armigera)
Baculovirus Genome analysis) In: Ignacimuthu A (eds) Biopesticides in insect pest manage-
ment, pp 199–207
Summers MD, Egawa K (1973) Physical and chemical properties of Trichoplusia ni granulosis
virus granulin. J Virol 12:1092–1103
Summers MD, Smith GE (1978) Baculovirus structural polypeptides. Virology 84:90–402
Summers MD, Smith GE, Knell JD, Burand JP (1980) Physical maps of Autographa californica
and Rachiplusia ou nuclear polyhedrosis virus recombinants. J Virol 34:693–703
Sun XL, Peng H (2007) Recent advances in biological pest insects by using viruses in China. Virol
Sin 22:158–162
Sundeep AB, Shouche YS, Mourya DT et al (2002) New Helicoverpa armigera Hbn cell line from
larval hemocyte for baculovirus studies. Indian J Exp Biol 40:69–73
Sundeep AB, Mourya DT, Mishra AC (2005) Insect cell culture in research: Indian scenario.
Indian J Med Res 121:725–738
Suzanne MT (2009) Baculovirus genes affecting host function. In Vitro Cell Dev Biol Anim
45:111–126
Szewczyk B, Hoyos-Carvajal L, Paluszek M et al (2006) Baculoviruses: re-emerging
biopesticides. Biotechnol Adv 24:143–160
Tanada Y, Kaya H (eds) (1993) Insect pathology. Academic, San Diego
Taun SJ, Tang LC, Hou RF (1989) Factors effecting Pathogenicity of NPV preparations to the corn
earworm, Heliothis armigera (Hubner). Entamophaga 34:541–549
Teakle RE, Byrne VS (1989) Nuclear polyhedrosis virus production in H. armigera infected at
different larval ages. J Invertebr Pathol 53:21–24
Teakle RE, Jensen JM, Giles JE (1985) Susceptibility of H. armigera to a commercial nuclear
polyhedrosis virus. J Invertebr Pathol 46:166–173
Thomas ED (1975) Normal virus levels and virus levels added for control. In: Summers M,
Engler R, Falcon LA, Vail P (eds) Baculoviruses for insect pest control: safety considerations.
Am Soc Microbiol, Washington, pp 87–89
Thorne CM, Otvos IS, Conder N, Levin DB (2007) Development and evaluation of methods to
detect the Choristoneura fumiferana nuclear polyhedrosis virus. Chinese J Virol 4:61–64
Tompkins GJ, Daugherty EM, Adams JR, Diggs D (1988) Changes in the virulence of nuclear
polyhedrosis virus when propagated in alternate Noctuid (Lepidoptera: Noctuidae) cell lines
and hosts. J Econ Entomol 81:1027–1032
Towbin H, Staehlin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide
gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A
76:4350–4354
Tuan SJ, Chen WL, Kao SS (1998) In vivo mass production and control efficacy of Spodoptera
litura (Lepidoptera: Noctuidae) nucleopolyhedrovirus. Chinese J Entomol 18:101–116
Tuan SJ, Kao SS, Cheng DJ, Hou RF, Chao YC (1999) Comparison of the characterization and
pathogenesis of three lepidopteran nucleopolyhedroviruses (HearNPV, SpeiNPV and
SpltNPV) isolated from Taiwan. Chinese J Entmol 19:167–186
Vail PV, Jay DL (1973) Replication and infectivity of the nuclear polyhedrosis virus of the alfalfa
looper, Autographa californica, produced in cells grown in vitro. J Invertebr Pathol 22:231–
237
Van Lent JWM, Groenen JTM, Klinge-Roode EC et al (1990) Localization of the 34 kDa
polyhedron envelope protein in Spodoptera frugiperda cells infected with Autographa
californica nuclear polyhedrosis virus. Arch Virol 111:103–114
Vanderzant ES, Richardson CD, Fort SWJ (1962) Rearing of the bollworm on artificial diet. J Econ
Entomol 55:140
Veenakumari K, Rabindra RJ, Srinivasa Naik CD et al (2006) Standardization of laboratory mass
production of Amsacta albistriga nucleopolyhedrovirus. J Biol Control 20:183–190
Veenakumari K, Rabindra RJ, Srinivasa Naik CD, Shubha MR (2007) In situ field level mass
production of Amsacta albistriga (Lepidoptera: Arctiidae) nucleopolyhedrovirus in a ground-
nut ecosystem in South India. Int J Trop Insect Sci 27:48–52
Vincent C, Andermatt M, Valéro J (2007) Madex® and VirosoftCP4®, viral biopesticides for
coddling moth control. In: Vincent C, Goethel MS, Lazarovits G (eds) Biological control: a
global perspective, Oxfordshire, UK, and Cambridge, USA: CAB International Virus taxon-
omy: seventh report of the international committee on taxonomy of viruses, pp 336–343
Vlak JM, Smith GE (1982) Orientation of the genome of Autographa californica nuclear polyhe-
drosis virus: a proposal. J Virol 41:1118–1121
Volkman LE, Falcon LA (1982) Use of monoclonal antibody in an enzyme-linked immunosorbent
assay to detect the presence of Trichoplusia ni (Lepidoptera: Noctuidae) single nuclear
polyhedrosis virus polyhedrin in T. ni larvae. J Econ Entomol 75:868–871
Volkman LE, Blissard GW, Friensen PD, Posse RD, Theilmann DA (1995) Baculovirudae. In:
Murphy F, Fauquet CM, Bishop DHL, Jarvis AW, Martelly G, Mayo E, Summers M D (eds)
Virus taxonomy: classification and nomenclature of viruses. Sixth report of the international
committee on taxonomy of viruses. Arch Virol 10:104–113
Weiser J (1987) Patterns over place and time. In: Fuxa JR, Tanada Y (eds) Epizootiology of insect
disease. Wiley, New York, pp 215–242
Wiegers F, Vlak JM (1984) Physical map of the DNA of a Mamestra brassicae nucleopolypoly-
hedrovirus variant isolates from Spodoptera exigua. J Gen Virol 65:2011–2019
Wigley PJ (1976) The epizootiology of a nuclear polyhedrosis virus disease of the winter moth,
Operophtera burmata L. at Wistman’s Wood, Dartmoor. D.Phil. Thesis, University of Oxford
William CF, Payne CC (1984) The susceptibility of Heliothis armigera (Hubner) larvae to three
nuclear polyhedrosis viruses. Ann Appl Biol 104:405–412
Williams T (1993) Covert iridovirus infection in blackfly larvae. Proc Roy Soc Lond B 251:225–
230
Winstanley D, O’Reilly D (1999) Granuloviruses. In: Webster RG, Granoff A (eds) Encyclopedia
of virology, 2nd edn. Academic, London, pp 69–84
Wolff JLC, Moraes RHP, Kitajima E, Leal EDS, Zanotto PMA (2002) Identification and charac-
terization of a baculovirus from Lonomia obliqua (Lepidoptera: Saturniidae). J Invertebr
Pathol 79:137–145
Wu C-Y, Wang C-H (2006) New cell lines from Lymantria xylina (Lepidoptera: Lymantriidae):
characterization and susceptibility to baculoviruses. J Invertebr Pathol 93:186–191
Yendol WG, Hamlen RA (1973) Ecology of entomogenous viruses and fungi. Ann N Y Acad Sci
217:18–30
Zanotto PMD, Kessing BD, Maruniak JE (1993) Phylogenetic interrelationships among
baculoviruses: evolutionary rates and host associations. J Invertebr Pathol 62:147–164
Zhang G, Kaupp WJ (1988) Use of monoclonal antibodies in an enzyme-linked immunosorbent
assay to detect the Choristoneura fumiferana nuclear polyhedrosis virus. Chinese J Virol 4:61–
64
Chapter 3
Bt Insecticidal Crystal Proteins:
Role in Insect Management
and Crop Improvement
K.Y. Srinivasa Rao, Debasis Pattanayak, and Rohini Sreevathsa
3.1 Introduction
Bacillus thuringiensis (Bt) is a gram-positive spore-forming bacterium naturally

occurring in all types of soils and all types of terrain including beaches, desert and
tundra habitats. Till date, 82 different serovars have been reported. Bt is a member
of the Bacillaceae family and belongs to the Bacillus cereus group.
B. thuringiensis is the most commercially successful biological control agent of
insect pests. According to Rowe and Margaritis (1987), toxin proteins from Bt
strains are classified into nine different types based on their site of toxicity. They are
α-exotoxin (phospholipase C), β-exotoxin (thermostable exotoxin), γ-exotoxin
(toxic to sawflies), δ-endotoxin (protein parasporal crystal), louse factor exotoxin
(active only against lice), mouse factor exotoxin (toxic to mice and Lepidoptera),
water-soluble toxin, Vip3A (Bt vegetative insecticidal protein) and enterotoxin
(produced by vegetative cells). Amongst several toxins produced by Bt strains,
δ-endotoxins have been more efficiently utilised for protection of a variety of crops
from various insect pests. In comparison to other commonly used insecticides, these
biological agents are safe for both the pesticide users and consumers of treated
crops. Among many bio-control agents, B. thuringiensis-based products have a
major share, i.e. up to 97 % of the bio-insecticides used worldwide.
B. thuringiensis produces vegetative insecticidal proteins (VIP) during vege-
tative phase and produces cry and cyt toxins as parasporal crystals during sporu-
lation phase; these crystals are commonly called as δ-endotoxins or pore-forming
toxins (PFT) or inclusion lesions (ILs). These protein families show toxic activity
against insects of different orders, viz., Lepidoptera, Coleoptera, Diptera and
against nematodes. The vip, cry and cyt toxins are activated by host proteases.
K.Y. Srinivasa Rao • D. Pattanayak • R. Sreevathsa (*)

National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute,
New Delhi 110012, India

DOI 10.1007/978-3-319-14499-3_3
54 K.Y. Srinivasa Rao et al.
These activated proteins lyse midgut epithelial cells by inserting into the target
membrane and form pores so that larvae die due to leakage of cell fluids. Among
this group of proteins, members of the three-domain cry family are used worldwide
for insect control, and their mode of action has been characterised. The other PFT,
i.e. colicins, exotoxin A, diphtheria toxins can affect mammals, but Cry and Vip
toxins have no effect on mammals due to lack of specific receptors. Cry toxins
interact with specific receptors, viz., aminopeptidase N (APN) receptors, cadherin-
like receptors and alkaline phosphatases (ALPs), located on the host cell surface
and are activated by host proteases following receptor binding resulting in the
formation of a pre-pore oligomeric structure that is insertion competent. In contrast,
Cyt toxins directly interact with membrane lipids and insert into the membrane.
Recent evidence suggests that Cyt synergise or overcome resistance to
mosquitocidal Cry proteins by functioning as a Cry membrane-bound receptor.
Several strains of B. thuringiensis can infect and kill insects due to the production of
insecticidal toxins during vegetative and sporulation phases. In the present scenario,
Bt is the only microbial insecticide in widespread use. The present review explains
the relevance and utility of Bt cry genes in plant biotechnology.
3.2 Historical Perspective
In 1901, a Japanese biologist Shigetane Ishiwatari isolated a bacterium while

working on the cause of sotto disease (killing large populations of silkworms),
and he named it as B. sotto. Later in 1911, Ernst Berliner isolated a bacterium that
killed a Mediterranean flour moth and named this bacterium as B. thuringiensis
(Siegel 2000) because the moth was first identified in the German state Thuringia.
He also reported that the existence of crystal within B. thuringiensis that spores and
crystals were coated onto leaves, the caterpillars stopped feeding and died. This led
to the discovery of insecticidal properties of the crystals and demonstrated that the
dead flour moth caterpillars were found to be loaded with spores and crystals.
In 1927, Mattes isolated Bt strain discovered by Ernst Berliner and conducted
subsequent field trials against the European corn borer (Ostrinia nubilalis). This
work eventually led to the development of Sporeine in 1938 (Luthy et al. 1982)
which was used as an insecticidal spray in France. Sporeine was the first commer-
cial Bt insecticide derived from kurstaki strain HD1 which was active against
lepidopteran pests (Baum et al. 1999). It was used primarily to kill flour moths
and was registered as an insecticide in the United States in 1961. During this period,
other Bt products such as Biobit, Dipel and Thuricide were derived from kurstaki
HD1, although other strains were also used to tackle lepidopteran (kurstaki SA-11,
kurstaki SA-12), dipteran (israelensis) and coleopteran (tenebrionis) pests (Kaur
2000). Until 1977, only 13 Bt strains had been described. All 13 subspecies were
toxic only to certain species of lepidopteran larvae. In 1977, the first subspecies
toxic to dipteran (flies) species was found, and in 1983, strains toxic to species of
coleopterans (beetles) were first discovered. In the 1980s, the use of Bt increased
3 Bt Insecticidal Crystal Proteins: Role in Insect Management and Crop Improvement 55
Table 3.1 Different Bt strains and its target insects

S. no. Insecticide obtained from Insecticide trade name Target insect
1 Bt kurstaki HD1 Thuricide Lepidoptera
2 Bt kurstaki SA-12 Costar Lepidoptera
3 Bt aizawai Florbac Lepidoptera
4 Bt israelensis Tekar Diptera
5 Bacillus sphaericus Vectolex Diptera
6 Bt tenebrionis Novodor Coleoptera
7 Bt tenebrionis Trident Coleoptera
due to harmful effects on the environment by chemical pesticides. Now there are
thousands of strains of Bt, and many of them have genes that encode unique toxic
crystals in their DNA (Table 3.1). With the advancement in molecular biology, it
soon became feasible to move the gene that encodes the toxic crystals into a plant.
The first genetically engineered plants: potato, corn and cotton were registered in
the Environmental Protection Agency (EPA), USA in 1995. Today, GM (geneti-
cally modified) crops including potato and cotton are planted throughout the world.
3.3 Bt Toxin Genes
The genes that produce toxic crystal proteins (Cry and Vip) during vegetative and
sporulation phases of B. thuringiensis are called as Bt genes. There are mainly two
different types of Bt toxins based on the phase of life cycle. The toxins produced
during sporulation are Cry toxin proteins (Table 3.2), and the ones produced during
vegetative phase are Vip toxin proteins (Fig. 3.1). Over time, many Bt toxins are
identified and are being used in many industries and research organisations around
the world (Schnepf et al. 1995; Federici 1999). In 1962, De Barjac first attempted to
classify Bt isolates based on the flagellar (H) agglutination (De Barjac and Bonnefoi
1962). More than 67 H-serotypes and 8 nonflagellated biotypes are now available,
and in many of these, the array of Bt toxin genes present is the same (Zeigler 1999).
In 1989, Hofte and Whitely classified Bt toxins based on homology of toxin gene
sequences and on the insecticidal activity into 42 Bt genes and 14 distinct types and
grouped them into four major classes. These classes are cry I (Lepidoptera specific),
cry II (Lepidoptera and Diptera specific), cry III (Coleoptera specific) and cry IV
(Diptera specific).
In 1998, Crickmore et al. (Table 3.2) introduced a systematic nomenclature for
classifying the cry and vip genes based on amino acid sequence of full-length gene
products rather than their biological properties. The cry genes designated by Hofte
and Whiteley (1989) have been retained, although the Roman numerals have been
replaced by Arabic numbers, i.e. cryII is now cry2, followed by uppercase and
lower case letters, i.e. cry2Aa, next followed by number, i.e. cry2Aa1.
The classification is based on amino acid sequence homology and four hierarchical
56
Table 3.2 Recent classification of cry genes identified so far from B. thuringiensis (http://www.lifesci.sussex.ac.uk/home/neil_crickmore/Bt/toxins2.html)
Class Subclass Class Subclass Class Subclass Class Subclass Cry Subclass
cry1 241 cry16 1 cry31 10 cry46 8 cry61 3
cry10 4 cry25 1 cry40 4 cry55 2 cyt1 12
cry11 7 cry26 1 cry41 4 cry56 2 cyt2 24
cry12 1 cry27 1 cry42 1 cry57 1
cry13 1 cry28 2 cry43 4 cry58 1
cry14 1 cry29 1 cry44 1 cry59 1
cry15 1 cry30 11 cry45 1 cry60 6
K.Y. Srinivasa Rao et al.
Fig. 3.1 Classification of

VIP
VIP genes formed during
vegetative phase of
VIP1 VIP3
B. thuringiensis (http://
www.lifesci.sussex.ac.uk/
home/Neil_Crickmore/Bt/ VIP2
vip.html/) 4 subgroups 3 subgroups
(Vip1a,Vip1b,Vip1c) (Vip3a,Vip3b,
2 subgroups Vip3c,Vip3d)
(Vip2a,Vip2b)
ranks consisting of numbers, capital letters, lower case letters and numbers
(e.g. cry1Aa1/vip1Aa1), depending on its position in a phylogenetic tree.
Currently, there are many collections composed of thousands of B. thuringiensis
isolates. Many of them may harbour novel cry genes, which may code for more
potent toxins or may show new biological activities (Porcar and Caballero 2000).
To date, more than 350 cry genes have been sequenced, which include major
groups, cry1 to cry51, and the list keeps growing (Crickmore et al. 1998, http://
www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/ access). The search for new
cry genes is of great importance, as this allows to find new toxins that could be more
potent or specific to the target larvae (Fernández-Larrea 2002).
Vegetative insecticide proteins are second generation of insecticides. Their
mode of action is similar to that of the other endotoxins but has no sequence
homology with any of the other known endotoxins. Till now, three different types
of vip genes are found, i.e. vip1, vip2, vip3. Among these genes, vip3 type of genes
are most abundant (67.4 %), followed by vip2 (14.6 %) and vip1 (8.1 %). vip3 is
specifically toxic to Lepidopteran pests, and vip1 and vip2 are specific to coleop-
teran pests.
vip genes can be classified based on amino acid sequence similarity into 3 groups,
9 subgroups, 25 classes and 82 subclasses (http://www.lifesci.sussex.ac.uk/home/
Neil_Crickmore/Bt/vip.html/).
3.4 Structure of Bt δ-Endotoxin
Bt δ-endotoxins are globular protein molecules, which accumulate as protoxins in

crystalline form during late stage of sporulation. Protoxins are liberated in the
midgut after solubilisation and are cleaved off at C-terminal part to release
66 kDa (approx.) active N-terminal toxic molecules. The protoxin contains well-
conserved cysteine residues (as many as 16 in cry1Ac), which helps in bridging
the protoxin molecules through intermolecular disulphide bonds and thereby
crystal formation.
Currently, three-dimensional protein structures have been determined through
X-ray crystallography. The tertiary structure of δ-endotoxins is comprised of three
distinct functional domains connected by a short conserved sequence. Each domain
of δ-endotoxin has independent and inter-related functions in the larval midgut,

which brings out colloid osmotic lysis (Knowles 1994). Phylogenetic analysis on
the domains of δ-endotoxins revealed that domain I is the most conserved, and
domain II is hyper variable among all δ-endotoxins (Bravo 1997). The analysis of
domain III sequences revealed a different topology due to the domain III swapping
among different types of toxins (Bravo 1997). The independent evolution of the
three structural domains and domain III swapping among different toxins generated
proteins with similar mode of action but with very different specificities (Bravo
1997).
The N-terminal domain (domain I) is a bundle of seven α-helices in which the
central helix-α5 is hydrophobic and is encircled by six other amphipathic-α helices,
and this helical domain is responsible for membrane insertion and pore formation.
Hence, domain I is called as pore-forming toxin (PFT). Domain II consists of three
antiparallel β-sheets with exposed loop regions, and domain III is a β-sandwich
(Li et al. 1991; Grochulski et al. 1995; Morse et al. 2001; Galitsky et al. 2001;
Boonserm et al. 2005, 2006). Exposed regions in domain II are involved in receptor
binding (Bravo et al. 2007), and domain III are involved in maintaining stability of
the total protein.
3.5 Mode of Action
Considerable amount of research on δ-endotoxin of B. thuringiensis has been

devoted to understand the mode of action on susceptible insects. The main insecti-
cidal activity of Bt is due to insecticidal crystalline inclusions formed during
sporulation (Beard et al. 2008). In general, following ingestion, the crystalline
inclusions are dissolved and then converted to active toxins by insect proteases.
The active toxins bind to specific receptor sites and produce pores in the midgut
epithelial membranes which results in loss of homeostasis and septicaemia, which
are lethal to the insect (Broderick et al. 2006).
The crystalline proteins formed during sporulation phase of B. thuringiensis are
protoxins, which do not have the toxic effect towards larvae. But when these
nontoxic crystalline inclusions (protoxins) are ingested by larvae, they dissolve in
high-pH (9.5) environment of larval midgut with the help of host proteases.
However, many coleopterans have a neutral pH midgut, yet solubilisation of the
coleopteran-specific toxins occurs (Koller et al. 1992). Host proteases enzymati-
cally convert protoxins into smaller active toxins which are resistant to further
protease digestion. These active toxins bind to unique receptors/binding sites on the
epithelium cells in the midgut of susceptible insects. So far, four different receptor/
binding proteins have been described in different lepidopteran insects: a cadherin-
like protein (CADR), a glycosylphosphatidylinositol (GPI)-anchored amino-
peptidase N (APN), a GPI-anchored ALP and a 270 kDa glycoconjugate (Knight
et al. 1994; Vadlamudi et al. 1995; Valaitis et al. 2001; Jurat-Fuentes and Adang
2004).
CADR is a transmembrane protein with cytoplasmic domain and extra cellular

ectodomain. The ectodomain contains calcium-binding proteins, integrin inter-
action sequences and cadherin protein-binding sequences. Surface plasmon reso-
nance experiments showed that the binding affinity of monomeric Cry1A toxins to
the Manduca sexta CADR is in the range of 1 nM (Vadlamudi et al. 1995), while
that of APN is in the range of 100 nM (Jenkins and Dean 2000).
Aminopeptidase N (APN) and ALP are external receptors of BBMV of larvae
which were anchored by a GPI. Surface plasmon resonance binding studies of
Cry1Ab mutants with pure M. sexta APN showed that domain II loop2 and loop3
are involved in APN recognition (Jenkins and Dean 2000). Cry1Ac domain III first
interacts with APN GalNAc sugar moieties facilitating the subsequent interaction
of domain II loop regions with another region in this receptor (Jenkins et al. 2000).
In M. sexta, proteomic analysis of BBMV Cry1Ac-binding proteins revealed that
ALP is a putative receptor molecule (McNall and Adang 2003).
After binding to the receptor, the toxin inserts irreversibly into the plasma
membrane of the cell leading to lesion formation. There is a positive correlation
between toxin activity and ability to bind brush border membrane vesicles epithelial
cells lining the gut of larvae (Gill et al. 1992). The toxicity is correlated with
receptor number rather than receptor affinity (Van Rie et al. 1989). The toxicity of
Bt lies in the organisation of α-helices derived from domain I. After binding to the
midgut epithelial cells, the α-helices can penetrate the apical membrane to form an
ion channel (Knowles and Dow 1993). The formation of toxin-induced pores in the
columnar cell apical membrane allows rapid fluxes of ions. The pores are K+
selective (Sacchi et al. 1986), permeable to cations (Wolfersberger 1989), anions
(Hendrickx and Estrada-Navarrete 1989) or to solutes such as sucrose irrespective
of the charge. Carroll and Ellar (1993) observed that midgut permeability in the
presence of Cry1Ac was altered for cations, anions, neutral solutes and water.
Knowles and Dow (1993) suggested that Bt toxins lead to cessation of K+ pump
leading to swelling of columnar cells and osmotic lysis. The disruption of gut
integrity leads to death of the insect through starvation or septicaemia. These
pores possess both selective (only K+ passes through) and nonselective (Na+ and
anions pass through) properties depending on the pH (Schwartz et al. 1993). The
lepidopteran insect midgut is alkaline and the pores probably permit K+ leakage.
Formation of this cation selective channel destroys the membrane potentials
(English and Slatin 1992) resulting in midgut necrosis, degeneration of peritrophic
membrane and epithelium and ultimately bacterial septicaemia. These pores allow
ions and water to pass freely into the cells, resulting in swelling, lysis and eventual
death of the host.
3.6 Importance of Bt Toxins
Till now there are mainly three different applications that have been achieved by
using Bt toxins. The first application is to control defoliator pests in forestry and
crops; second application is to control mosquitoes because these are the vectors of
many dangerous human diseases and third application is development of transgenic
insect-resistant crops.
3.6.1 Bt Toxin as Bioinsecticide Spray
One of the most successful applications of Bt has been the control of lepidopteran
defoliators by Bt sprays. The lepidopteran defoliators are pests of coniferous forests
mainly in Canada and the USA. In both countries, the control of forests defoliators
relies mostly on the use of Bt strain, kurstaki HD1, producing Cry1Aa, Cry1Ab,
Cry1Ac and Cry2Aa toxins (van Frankenhuyzen 2000; Bauce et al. 2004). Success-
ful application of Bt is highly dependent on proper timing, weather conditions and
high dosage of spray applications. These factors combine to determine the proba-
bility of larvae ingesting lethal dose (van Frankenhuyzen 2000; Bauce et al. 2004).
The use of Bt in the control of defoliators has resulted in a significant reduction in
the use of chemical insecticides for pest control in the forests. Most Bt products
which are used as insecticides are derived from kurstaki strain HD1 (e.g. Biobit,
Dipel and Thuricide), and other strains are used to tackle lepidopteran (kurstaki
SA-11, kurstaki SA-12), dipteran (israelensis) and coleopteran (tenebrionis) pests
(Kaur 2000) (Table 3.1).
In 1960, strain improvement led to the replacement of many of the early products
with new Bt strains that were up to tenfold more potent than their predecessors. It
can be achieved by creating new bacterial strains carrying unknown combinations
of existing toxins by conjugation or direct transformation (Gonzalez et al. 1982;
Kronstad et al. 1983; Carlton and Gonzalez 1985).
Below are two examples of developing combinational pesticides by conjugation
process. First is Foil, a product which was achieved by conjugation process between
strain EG2424, which produces Cry1Ac toxin from Bt kurstaki, which is active
against the European corn borer, and Cry3A toxin from Bt tenebrionis, which is
active against the Colorado potato beetle, Leptinotarsa decemlineata (Carlton and
Gawron-Burke 1993). Second example is Tobaggi, a product obtained from Bt
strain NT0423 developed by Dongbu Hannong Chemicals, Korea. This strain had
five known crystal protein genes cry1Aa, cry1Ab, cry1C, cry1D and cry2A and one
new gene cry 1Af1 (GenBank Accession No. U82003). It has a dual toxicity against
lepidopteran larvae-like Plutella xylostella, Spodoptera exigua and Hyphantria
cunea and dipteran larvae-like Culex pipiens and Musca domestica. Today, the Bt
biopesticide market is dominated by Abbott Laboratories (Chicago, IL) (since the
acquisition in 1995 of Novo Nordisk’s biopesticide business) and Novartis (created
through the merger in 1996 of Ciba and Sandoz), together accounting for >70 % of
global production. The other 30 % is divided among approximately 30 companies
with over 100 different Bt product formulations, most containing a single Bt toxin
but some combining up to five.
There are some advantages and some disadvantages of using biopesticides as
sprays. Advantages:
• The biopesticide has potent insecticidal activity.
• Has host specificity.
• Less costly and takes less time to develop bioinsecticide than chemical
insecticide.
• Harmless to humans and other mammals.
• Biodegradable.
Disadvantages:
• Biopesticides are rapidly inactivated by UV, heat and extreme pH.
• It is easily removed from plant surface by wind and rain.
• These biopesticides are also susceptible to proteases in leaf exudates of plants.
3.6.2 Use of Bt Toxin to Control Mosquitoes
Many diseases of humans are caused through vectors such as mosquitoes. Bt toxins
are highly active against mosquitoes like A. aegypti, which is a vector of dengue
fever, Simulium damnosum is a vector of onchocerciasis and certain Anopheles
species are vectors of malaria. Bt toxin is used as an alternative control method of
mosquito and black fly populations (Becker 2000) due to its high insecticidal
activity, lack of toxicity to nontarget organisms and lack of showing insect-resistant
populations to chemical insecticides like DDT. In 1983, a control programme for
the eradication of onchocerciasis was launched in 11 countries of Western Africa
using Bt toxin. Presently, more than 80 % of this region is protected by Bt toxin
applications and 20 % with the chemical larvicide, temephos. Furthermore, control
of onchocerciasis has protected over 15 million children without the appearance of
black fly resistance to Bt toxin (Guillet et al. 1990). This success of vector control
using Bt toxin will certainly increase its use around the world.
3.7 Bt Transgenic Crops
The development of transgenic crops that produce Bt Cry toxins has been a major
breakthrough in the substitution of chemical insecticides by environmental friendly
alternatives. The main advantage of transgenic crops is that the Bt toxin is always
produced in plant without any degradation. In 1987, first Bt transgenic plants were
developed in tomato and tobacco (Barton et al. 1987; Fischhoff et al. 1987; Vaeck
et al. 1987). The full-length or truncated Bt toxin genes (cry1A) were used in tomato
and tobacco, and the gene was transformed from Bt var. kurstaki HD1 which is
toxic to lepidopteran pests. But expression of the toxin protein was very poor in the
tobacco plants, and the mortality of M. sexta larvae was only 20 %.
In 1990, researchers at Monsanto made a significant advancement in the expres-
sion of Bt genes in plants (Perlak et al. 1990). They noticed that Bt genes were
excessively AT rich in comparison with normal plant genes. This bias in nucleotide
composition of the DNA could have a number of deleterious consequences to gene
expression because AT-rich regions in plants are often found in introns or have a
regulatory role in determining polyadenylation. In addition, plants have a tendency
to use G or C in the third base of redundant codons, A or T being rarer. By
considering all these codon modifications, the low expression could be overcome in
plants. The plants have been improved by engineering cry genes with a plant-biased
codon usage by removal of putative splicing signal sequences and deletion of the
carboxy-terminal region of the protoxin (Schuler et al. 1998).
Perlak et al. (1990) made a gene construct in which the first 1,359 nucleotides
were derived from fully modified cry1Ab gene and the remaining sequence from
partially modified cry1Ac gene. The variant gene was placed under the control of
CaMV 35S promoter containing a duplicated enhancer region. The toxin protein
levels in transgenic tobacco and tomato harbouring these modified genes increased
up to 100-fold over levels seen with the wild-type Bt gene in plants. Cotton variety
Coker 312 was transformed, and the transgenic plants were shown to have total
protection from Trichoplusia ni (cabbage looper), S. exigua and Helicoverpa zea
(cotton bollworm).
In the past few years, more than 30 plant species have been transformed by using
a range of modified Bt genes (Schuler et al. 1998; de Maagd et al. 1999). The use of
insect-resistant crops has diminished considerably the use of chemical pesticides in
areas where these transgenic crops are planted (Qaim and Zilberman 2003). The
first commercial crop was potato; the transgenic potato harbouring cry3A gene from
Bt var. tenebrionis was shown to protect the crop against Colorado potato beetle in
the field much more efficiently than Cry3A topical sprays. Many commercially
useful crops like rice, maize and cotton have since been developed (Perlak
et al. 1993) (Table 3.3).
In 1995, the US EPA approved the first registration of Bt potato, corn and cotton
crops. The first to reach the market was Monsanto’s NewLeaf potato variety
expressing cry3A gene, followed by two transgenic corn hybrids expressing
cry1Ab gene to protect against the European corn borer, i.e. KnockOut by Syngenta
(Basel, Switzerland) and NatureGard by Mycogen (both containing event 176).
Monsanto released the cotton varieties Bollgard and Ingard (events 531, 757 and
1076) expressing a modified Cry1Ac toxin. NewLeaf potato and its successors
(NewLeaf Y and NewLeaf Plus) were withdrawn from the market in 2002, and corn
varieties containing event 176 were later withdrawn and replaced with more
profitable products. Commercially important crops harbouring Bt genes and their
resistance have been shown in Table 3.4.
Table 3.3 Some important S. no. Bt toxin Crop Target pest

Cry toxins used initially to
generate GM crops 1 Cry1A Tomato Pinworm
2 Cry1A Tobacco Helicoverpa zea
3 Cry1A Potato Tuber moth
4 Cry3A Potato Colorado potato beetle
5 Cry1A Cotton Pink bollworm
6 Cry1A Maize European corn borer
7 Cry1A Rice Stem borer
Table 3.4 Use of transgenic Bt gene in commercially important crops against insect pests
S. no. Bt gene Active against insect pest Crop
1 cry1Ab, cry1Ac Bollworms Cotton
2 cry1Ab, cry9C European corn borer Maize
3 cry3Aa Colorado potato beetle Potato
4 cry1Ab Tuber moth Potato
5 cry1Ac, cry2Aa Yellow stem borer Rice
6 cry1Ab Eight lepidopterans Rice
7 cry1Ac Yellow stem borer Basmati rice
8 cry1Ac Fruit borer Tomato
9 cry1Ab Fruit borer Brinjal
10 cry1Ac Diamondback moth Cabbage
11 cry1C Diamondback moth Broccoli
12 cry1Ac Diamondback moth Canola
13 cry1C Beet armyworm Alfalfa
14 cry1Ac Leaf miner Coffee
15 cry1Ac Cornstalk borer Peanut
Since 1996, plants have been modified with short sequences of genes from Bt to
express the crystal protein. With this method, plants themselves can produce the
proteins and protect themselves from insects without any external Bt sprays or
synthetic pesticide sprays. In 1999, 29 million acres of Bt corn, potato and cotton
were grown globally. It has been estimated that by using Bt protected cotton, the
United States was able to save approximately $92 million.
The genetic engineering companies with interests in Bt plants include the
following:
Agracetus (USA), Agricultural Genetics Co Ltd (UK), Agrigenetics Advanced
Sciences Co (USA subsidiary of Lubrizol), Ciba-Geigy (Switzerland, now
Novartis), DeKalb (USA), Monsanto (USA), Plant Genetic Systems (Belgium)
and Sandoz (Switzerland, now Novartis). By 1997, these companies were field-
testing at least 18 different Bt crops.
From 1998 onwards, the Bt crop production has increased because the EPA had
approved an insect-resistant tomato line expressing cry1Ac. In 2002, the Herculex
corn variety was developed jointly by Pioneer Hi-Bred and Dow Agrosciences
Table 3.5 Events approval for field trials of GM crops happened in 2013
Company
S. no. Gene/event Trait Crop name
1 cp4epsps/MON 88913 Herbicide RRF Cotton Maharashtra
tolerance Hybrid Seeds
Company Ltd.
2 Events Bt11, GA21 and stack of Insect resis- Corn Syngenta Bio-
Bt11 GA21 tance and her- sciences Pvt.
bicide Ltd.
tolerance
Bt11, GA21 and stack event of Insect resis- Syngenta Bio-
Bt11 GA21 tance and her- sciences Pvt.
bicide Ltd.
tolerance
Bt11 and GA21 Insect resis- Syngenta Bio-
tance and her- sciences Pvt.
bicide Ltd.
tolerance
cry2Ab2 and cry1A.105 genes Insect Monsanto
(Event MON 89034) resistance India Ltd.
3 cp4epsps (Event NK603) Herbicide Herbicide- Monsanto
tolerance tolerant India Ltd.
maize
4 Stacked events, namely, GHB119 Insect TwinLink® Bayer Biosci-
(cry2Ae/PAT) & T304-40 (cry1Ab/ resistance Cotton ence Pvt Ltd
PAT) containing cry1Ab, cry2Ac and
bar
5 2mepsps (Event GHB 614) Herbicide Herbicide- Bayer Biosci-
tolerance tolerant ence Pvt Ltd
Glytol
cotton
expressing cry1F, which protects plants from black cutworm (Agrotis ipsilon) and
European corn borer. After that EPA approved Monsanto’s Bollgard II cotton
variety expressing two Bt toxins cry1Ac and cry2Ab. The first stacked variety
developed by Monsanto YieldGard Plus expressing cry1Ab1 and cry3Bb1 was
released in 2003. Some of events with Bt gene, which were given permission for
field trials in India during 2013, are shown in Table 3.5. At present, the USA has the
largest area for Bt crops followed by Argentina, Brazil, India, Canada, China and
other countries (Table 3.6). Further, Bt crops are grown in 28 countries, and the total
area globally under Bt crop cultivation in 2013 was 175 million hectares. Some
examples of commercially important Bt crops are Bt corn, Bt cotton and Bt rice.
Bt cotton: The cotton crop in the USA is damaged by lepidopteran larvae,
particularly by cotton bollworm (H. zea), pink bollworm (Pectinophora
gossypiella) and tobacco budworm (Heliothis virescens). To reduce the damage
by Helicoverpa larvae, the US government approved cultivation of Bt cotton.
Firstly a single transformation line Monsanto 531 was developed by Monsanto
Table 3.6 Current scenario of GM crops in the world (total 28 countries)

Area grown by
GM crops
(in million ha)
S. no. Country 2012 2013 Major crops
1 USA 69.5 70.1 Maize, soya bean, cotton, sugar beet, papaya, squash,
canola
2 Brazil 36.6 40.3 Soya bean, maize, cotton
3 Argentina 23.9 24.4 Soya bean, maize, cotton
4 India 10.8 11.0 Cotton
5 Canada 11.6 10.8 Canola, maize, soya bean, sugar beet
6 China 4 4.2 Rice, cotton, papaya, tomato, sweet pepper
7 Other 13.9 14.4
countries
Total 28 countries 170.3 175.2
Source: Clive James, February 17, 2014
and marketed as Bollgard cotton. These Bollgard cotton lines contain a synthetic
cry1Ac gene derived by an enhanced CaMV 35S promoter. By using these lines in
the USA, a remarkable difference in the production has been observed, because of
this the acreage of Bt cotton has risen from 12 % in 1996 to 35 % in 2000. Since
then, there has been a constant raise in the adoption of the technology in various
countries worldwide. Major thrust is from developing countries with the resource-
poor farmers being highly benefited from the technology in general and Bt cotton
specifically (James 2012). The cultivation of Bt cotton has reduced the number of
pesticide sprays on the cotton crop, thereby improving farmers’ health. Benefits
have also been visualised economically in various countries (Brookes and Barfoot
2013). The extent of adoption of the technology demonstrates that technology is
here to stay.
Bt corn was also developed to control lepidopteran insects. Corn is mostly
damaged by European corn borer complex (Ostrinia nubilalis), southwestern corn
borer [Diatraea grandiosella (Dyar)] and sugar cane borer [Diatraea saccharalis
(Fabricus)] (Shelton et al. 2002). As for the European corn borer (O. nubilalis), as
the name suggests, the larvae damage maize crops by tunnelling into the central pith
of stalks. The European corn borer-resistant maize has been developed to reduce the
damage of corn by corn borer larvae. Two different companies, i.e. Novartis and
Monsanto, developed three different lines showing resistance against corn borer
larvae. The transgene cry1Ab was used in all three different lines with different
promoters. Bt11 line commercially called as YieldGard was developed by Novartis,
Mon810 event was developed by Monsanto and another line Bt176 event, commer-
cially called as KnockOut, was developed by Novartis. Among these three lines, the
first two had cry1Ab construct controlled by constitutive promoter, and the knock-
out line was developed by bombarding with two separate cry1Ab constructs con-
trolled by maize PEP carboxylase promoter. By using these lines, the production of
corn increased drastically and the growing area of corn also increased from 5 % in
1996 to 25 % in 2000. By 2012, Bt maize occupied 7.5 million hectares, up by 1.5

million hectares from 2011 with a growth of 25 % and equivalent to 4 % of global
biotech area (James 2012). Of late, there have been a large number of single and
stacked events of corn: cry1A.105 (MON89034), cry34Ab1 (59122), cry35Ab1
(59122), cry1F (1507), cry2Ab (MON89034), cry3Bb1 (MON863 and
MON88017), mcry3A (MIR604) and vip3A (MIN162). The corn which was geneti-
cally modified to produce VIP was first approved in the USA in 2010.
Bt rice was first developed by China and field trials of Bt rice were conducted in
China in 1998. A number of Bt lines were developed containing modified cry1A,
cry1Ab and cry1Ac genes (Huang et al. 2007). Rice stem borer is a major group of
pest of rice and causes annual losses of 11.5 billion yuan (US$1.69 billion). In 1989,
scientists from Chinese Academy of Agricultural Sciences (CAAS) by using PEG
generated the first insect-resistant genetically modified rice plant with cry genes
under the control of CaMV 35S promoter. Later in 2009, China developed two
commercially important Bt rice lines containing cry1Ab/Ac gene with the names
Huahui No. 1 and Bt Shanyou 63. The Chinese government is currently undertaking
trials on insect-resistant cultivars. The benefit of this is that the farmers do not need
to spray their crops with pesticides to control fungal, viral or bacterial pathogens.
In comparison, conventional rice is sprayed three or four times per growing season.
Benefits of growing Bt rice include increased yield and thereby boost in the
economy. It is expected that India, Indonesia and the Philippines would carry out
the cultivation of genetically modified rice.
3.8 Importance of Resistance Management Strategy
Development of resistance by the target pest or pathogen is an important aspect of

pest management (Bates et al. 2005; van der Salm et al. 1994). In the past, it has
been observed that mosquito could develop resistance to DDT, a chemical insecti-
cide. This can happen in the case of biopesticides as well. There are many factors
that are involved in the development of resistance against insecticides, i.e. in a
population of insects, if some insects carry genes for resistance, they produce
progenies with the resistant gene.
To avoid problems with resistance of insects against insecticides in transgenic
crops, farmers have to follow resistance management requirements set by EPA.
These include the use of more than one transgene (pyramiding), domain swapping,
crop rotation and refuge planting as a part of resistant management in transgenic
farming. Pyramiding is a process of stacking two or more transgenes in a population
by conventional crossing between two different transgenic lines. Domain swapping
or domain engineering is a process to increase the toxicity of cry genes by
producing chimeric proteins. For example, cry1AcF gene, the first and second
domain of cry1Ac gene and third domain of cry1F, is constructed by domain
engineering. It shows effective resistance against a wide range of insects.
Crop rotation is a method to combat resistance of insects against GM crops. Due

to crop rotation, the pressure of one specific strain of Bt on a specific insect will be
minimised due to less time availability for development of resistance.
Refuge planting is a method of planting in the field in which Bt crops are planted
with alternating rows of non-Bt crops. The reason behind this type plantation is
according to laws of genetics, insects that have developed resistance to Bt have
more chances of mating with an insect that has not developed resistance to Bt and
that the progenies of insects do not become resistant to Bt. The EPA has set some
guidelines for refuge planting in refuge area, i.e.:
• Growers may plant up to 80 % area of their Bt crop and at least 20 % must be
planted with non-Bt crop.
• Refuge area must be within, adjacent to or near the Bt crop. It must be within half
mile from Bt crop.
• If refuge areas are strips within a field, the strips should be at least four rows.
There are different types of refuge planting, which include block design, linear
block design, bracket design, border (perimeter) design and strips (split planter). In
block design, one block in the Bt field must be planted with non-Bt plants. In linear
block design, one side (20 %) of the Bt field must be planted with non-Bt. In bracket
design, both sides of the Bt field must be planted with non-Bt plants. In border
design, all sides of Bt crop must be accompanied with non-Bt plants. In strips
design, the non-Bt plantation has to be done between Bt plantation as strips.
3.9 Conclusion
The Bt ICPs have infused tremendous potential in helping the plants combat various
insect pests. The Bt cotton saga is proof enough for the success of these genes.
There is a continued increase in the adoption of the technology worldwide with
various food and nonfood crops being transformed. Further, fishing for effective cry
genes is also still on the move. The studies and the effective demonstrations of the
technology will have immense value based on the acceptance of the technologies
and products. An initial boost will enable various transgenics in the pipeline to be
introduced to mankind. Care should be however taken in practising the right insect
management practices for the successful utilisation of the painstaking efforts.
References
Barton KA, Whiteley HR, Yang NS (1987) Bacillus thuringiensis δ-endotoxin expressed in
transgenic Nicotiana tabacum provides resistance to lepidopteran insects. Plant Physiol 85:
1103–1109
Bates SL, Zhao JZ, Roush RT, Shelton AM (2005) Insect resistance management in GM crops:
past, present and future. Nat Biotechnol 23:57–62
Bauce E, Carisey N, Dupont A, van Frankenhuyzen K (2004) Bacillus thuringiensis subsp kurstaki
(Btk) aerial spray prescriptions for balsam fir stand protection against spruce budworm
(Lepidoptera: Tortricidae). J Econ Entomol 97:1624–1634
Baum JA, Johnson TB, Carlton BC (1999) Bacillus thuringiensis: natural and recombinant
bioinsecticide products. In: Hall FR, Menn JJ (eds) Biopesticides: use and delivery. Humana
Press, Totowa, pp 189–210
Beard CE, Court L, Mourant RG, James B, Van Rie J, Masson L, Akhurst RJ (2008) Use of a
cry1Ac-resistant line of Helicoverpa armigera (Lepidoptera: Noctuidae) to detect novel
insecticidal toxin genes in Bacillus thuringiensis. Curr Microbiol 57:175–180
Becker N (2000) Bacterial control of vector-mosquitoes and black flies. In: Entomopathogenic
bacteria: from laboratory to field application. Kluwer Academic, Dordrecht, pp 383–396
Boonserm P, Davis P, Ellar DJ, Li J (2005) Crystal structure of the mosquito-larvicidal toxin
Cry4Ba and its biological implications. J Mol Biol 348:363–382
Boonserm P, Mo M, Angsuthanasombat C, Lescar J (2006) Structure of the functional form of the
mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-Å resolution. J Bacteriol
188:3391–3401
Bravo A (1997) Phylogenetic relationships of Bacillus thuringiensis delta-endotoxin family pro-
teins and their functional domains. J Bacteriol 179:2793–2801
Bravo A, Gill SS, Soberon M (2007) Mode of action of Bacillus thuringiensis Cry and Cyt toxins
and their potential for insect control. Toxicon 49:423–435
Broderick NA, Raffa KF, Handelsman J (2006) Midgut bacteria required for Bacillus thuringiensis
insecticidal activity. Proc Natl Acad Sci U S A 103:15196–15199
Brookes G, Barfoot P (2013) GM crops: global socio-economic and environmental impacts
1996–2011. PG Economics Ltd, Dorchester
Carlton BC, Gawron-Burke C (1993) Genetic improvement of Bacillus thuringiensis for
bioinsecticide development. In: Kim L (ed) Advanced engineered pesticides. Marcel Dekker,
Carlton ΒC, Gonzalez JΝ (1985) Plasmids and delta-endotoxin production in different subspecies
of Bacillus thuringiensis. In: Hoch JA, Setlow P (eds) Molecular biology of microbial
differentiation. American Society for Microbiology, Washington, DC, pp 246–252
Carroll J, Ellar DJ (1993) An analysis of Bacillus thuringiensis δ-endotoxin action on insect-
midgut-membrane permeability using a light scattering assay. Eur J Biochem 214:771–778
Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van Rie J, Lereclus D, Baum J, Dean DH (1998)
Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins.
Microbiol Mol Biol Rev 62:807–813
De Barjac H, Bonnefoi A (1962) Essai de classiffication biochimique et serologique de 24 souches
de Bacillus du type B. thuringiensis. Entomophaga 7:5–31
De Maagd RA, Bakkar PL, Masson L, Adang MJ, Sangandala S, Stiekema W, Bosch D (1999)
Domain III of the Bacillus thuringiensis delta-endotoxin Cry1Ac is involved in binding to
Manduca sexta brush border membranes and to its purified amino peptidase N. Mol Microbiol
31:463–471
English L, Slatin SL (1992) Mini-review. Mode of action of Delta-endotoxins from Bacillus
thuringiensis: a comparison with other bacterial toxins. Insect Biochem Mol Biol 22:1–7
Federici BA (1999) Bacillus thuringiensis in biological control. In: Bellows TS, Fisher TW (eds)
Handbook of biological control: principles and applications, Chapter 21. Academic,
San Diego, pp 575–593
Fernández-Larrea O (2002) Tecnologı́as de producci on de Bacillus thuringiensis. Manejo
Integrado de Plagas y Agroecologı́a 64:110–115
Fischhoff DA, Bowdish KS, Perlak FJ, Marrone PG, McCoormick SM, Niedermeyer JG,
Dean DA, Kusano KK, Mayer EJ, Rochester DE, Rogers SG, Fraley RT (1987) Insect tolerant
transgenic tomato plants. Biotechnology 5:807–813
Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L (2001) Structure of
the insecticidal bacterial δ-endotoxin CryBb1 of Bacillus thuringiensis. Acta Crystallogr D
Biol Crystallogr D 57: 1101–1109
Gill SS, Cowles EA, Pietrantonio PV (1992) The mode of action of Bacillus thuringiensis
endotoxins. Annu Rev Entomol 37:615–636
Gonzalez JM, Brown BJ, Carlton BC (1982) Transfer of Bacillus thuringiensis plasmids coding for
delta-endotoxin among strains of B. thuringiensis and B. cereus. Proc Natl Acad Sci USA 79:
6951–6955
Grochulski P, Masson L, Borisova S, Pusztai-Carey M, Schwartz JL, Brousseau R, Cygler MJ (1995)
Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation.
Mol Biol 254:447–464
Guillet P, Kurtak DC, Philippon B, Meyer R (1990) Use of Bacillus thuringiensis israelensis for
Onchocerciasis control in West Africa. In: de Barjac H, Sutherland D (eds) Bacterial control of
mosquitoes and black flies: biochemistry, genetics, and applications of Bacillus thuringiensis
israelensis and Bacillus sphaericus. Rutgers Univ. Press, New Brunswick, pp 187–201
Hendrickx ME, Estrada-Navarrete FD (1989) A checklist of the species of pelagic shrimps
(Penaeoidea and Caridea) from the eastern Pacific with notes on their zoogeography and
depth distribution. CalCoFi Rep 30:104–111
Hofte H, Whiteley HR (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev
53:242–255
Huang F, Leonard BR, Andow DA (2007) Sugarcane borer (Lepidoptera: Crambidae) resistance to
transgenic Bacillus thuringiensis maize. J Econ Entomol 100:164–171
James C (2012) Global status of commercialised biotech D GM Crops: 2012. ISAAA Brief 44.
ISAAA, Ithaca
Jenkins JL, Dean DH (2000) Exploring the mechanism of action of insecticidal proteins by
genetic engineering methods. In: Setlow JK (ed) Genetic engineering: principles and methods.
Plenum, New York, p 33
Jenkins JL, Lee MK, Valaitis AP, Curtiss A, Dean DH (2000) Bivalent sequential binding model
of a Bacillus thuringiensis toxin to gypsy moth aminopeptidase N receptor. J Biol Chem 275:
14423–14431
Jurat-Fuentes JL, Adang MJ (2004) Characterization of a Cry1Ac-receptor alkaline phosphatase
susceptible and resistant Heliothis virescens larvae. Eur J Biochem 271:3127–3135
Kaur S (2000) Molecular approaches towards development of novel Bacillus thuringiensis
biopesticides. World J Microbiol Biotechnol 16:781–793
Knight P, Crickmore N, Ellar DJ (1994) The receptor for Bacillus thuringiensis CryIA(c) delta-
endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase
N. Mol Microbiol 11:429–436
Knowles BH (1994) Mechanism of action of Bacillus thuringiensis Insecticidal d-endotoxins.
Adv Insect Physiol 4:275–308
Knowles BH, Dow JAT (1993) The crystal d-endotoxins of Bacillus thuringiensis: models for their
mechanism of action on the insect gut. Bioessays 15:469–476
Koller CN, Bauer LS, Hollingworth RM (1992) Characterization of the pH-mediated solubility of
Bacillus thuringiensis var. san diego native delta endotoxin crystals. Biochem Biophys Res
Commun 184:692–699
Kronstad J, Schnepf WHE, Whiteley HR (1983) Diversity of locations for Bacillus thuringiensis
crystal protein genes. J Bacteriol 154:419–428
Li J, Carroll J, Ellar DJ (1991) Crystal structure of insecticidal d-endotoxin from Bacillus
thuringiensis at 2.5 Å resolutions. Nature 353:815–821
Luthy P, Cordier JL, Fischer HM (1982) Bacillus thuringiensis as a bacterial insecticide:
basic considerations and application. In: Kurstak E (ed) Microbial and viral pesticides.
Marcel Dekker, New York/Basel, pp 35–74
McNall RJ, Adang MJ (2003) Identification of novel Bacillus thuringiensis Cry1Ac binding
proteins in Manduca sexta midgut through proteomic analysis. Insect Biochem Mol Biol 33:
999–1010
Morse RJ, Yamamoto T, Stroud RM (2001) Structure of Cry2Aa suggests an unexpected receptor
binding epitope. Structure 9:409–417
Perlak FJ, Deaton RW, Armstrong RL, Fuchs RL, Sims SR, Greenplate JT, Fischhoff DA (1990)
Insect resistant cotton plants. Biotechnology 8:939–943
Perlak FJ, Stone TB, Muskopf YM, Petersen LJ, Parker GB, McPherson SA, Wyman J, Love S, Reed G
(1993) Genetically improved potatoes: protection from damage by Colorado potato beetles.
Plant Mol Biol 22:313–321
Porcar M, Caballero P (2000) Molecular and insecticidal characterization of a Bacillus
thuringiensis strain isolated during a natural epizootic. J Appl Microbiol 89:309–316
Qaim M, Zilberman D (2003) Yield effects of genetically modified crops in developing countries.
Science 299:900–902
Rowe GE, Margaritis A (1987) Bioprocess developments in the production of bioinsecticides by
Bacillus thuringiensis. CRC Crit Rev Biotechnol 6:87–127
Sacchi VF, Parenti P, Hanozet GM, Giordana B, Luethy P, Wolfersberger MG (1986) Bacillus
thuringiensis toxin inhibits K-gradient-dependent amino acid transport across the brush border
membrane of Pieris brassicae midgut cells. FEBS Lett 204:213–218
Schnepf E, Crickmore N, Rie JV, Lereclus D, Baum J, Feitelson J, Zeigler DJ, Dean DH (1995)
Schuler TH, Poppy GM, Kerry BR, Denholm I (1998) Insect-resistant transgenic plants. Trends
Schwartz JL, Garneau L, Savaria D, Masson L, Brousseau R, Rousseau E (1993) Lepidopteran-
specific crystal toxins from Bacillus thuringiensis form cation- and anion-selective channels in
planar lipid bilayers. J Membr Biol 132:53–62
Shelton AM, Zhao JZ, Roush RT (2002) Economic, ecological, food safety, and social conse-
quences of the deployment of Bt transgenic plants. Annu Rev Entomol 47:845–881
Siegel JP (2000) Bacteria. In: Lacey LL, Kaya HK (eds) In field manual of techniques in
invertebrate pathology. Kluwer, Dordrecht, pp 209–230
Vadlamudi RK, Weber E, Ji I, Ji TH, Bulla LA (1995) Cloning and expression of a receptor for an
insecticidal toxin of Bacillus thuringiensis. J Biol Chem 270:5490–5494
Vaeck M, Reynaerts A, Hofte H, Jansens S, Beukeleer MD, Dean C, Zabeau M, Montagu MV,
Leemans J (1987) Transgenic plants protected from insect attack. Nature 328:33–37
Valaitis AP, Jenkins JL, Lee MK, Dean DH, Garner KJ (2001) Isolation and partial character-
ization of Gypsy moth BTR-270 an anionic brush border membrane glycoconjugate that binds
Bacillus thuringiensis Cry1A toxins with high affinity. Arch Insect Biochem Physiol 46:
186–200
van der Salm T, Bosch D, Honée G, Feng L, Munsterman E, Bakker P, Stiekema WJ, Visser B
(1994) Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA
(b) and cryIC genes: a resistance management strategy. Plant Mol Biol 26:51–59
Van Frankenhuyzen K (2000) Application of Bacillus thuringiensis in forestry. In: Charles JF,
Delécluse A, Nielsen-LeRoux C (eds) Entomopathogenic bacteria: from laboratory to field
application. Kluwer Scientific publishers, Dordrecht, Netherlands, pp. 371
Van Rie J, Jansens S, Hofte H, Degheele D, Van Mellaert H (1989) Specificity of Bacillus
thuringiensis δ-endotoxins. Eur J Biochem 186:239–247
Wolfersberger MG (1989) Neither barium nor calcium prevents the inhibition by Bacillus
thuringiensis δ-endotoxin of sodium or potassium gradient-dependent amino acid accumu-
lation by tobacco hornworm midgut brush border membrane vesicles. Arch Insect Biochem
Physiol 12:267–277
Zeigler DR (1999) Bacillus Genetic Stock Center catalog of strains. Part 2: Bacillus thuringiensis
and Bacillus cereus, 7th edn. Bacillus Genetic Stock Center, Columbus
Chapter 4
Identification and Characterization
of Receptors for Insecticidal Toxins from
Bacillus thuringiensis
Ricardo A. Grande-Cano and Isabel Gomez
4.1 Introduction
During the stationary phase of the life cycle of Bacillus thuringiensis (Bt) is
produced a parasporal body (crystal) containing insecticidal proteins called Cry
toxins, which are used for development of transgenic crops and spray formulations
for control of pest insects (Van Rie 2000). Although Bt has been used for two
decades as a commercial biopesticide, the mode of action has only been described
recently. Cry proteins are included into the pore-forming toxins (PFTs), one of the
largest classes of bacterial toxins (Bravo et al. 2011). Many sequences of cry genes
have been identified, including 56 families (Cry1–Cry56) and 180 subtypes
(Cry1Aa, Cry4Ba, etc.). Of all of these proteins, some structures have been resolved
by X-ray crystallography. It is interesting to note that the sequences are different but
the structural topology is conserved, indicating a similar mechanism of action. All
contain three structural domains: Domain I contains seven α-helixes and is involved
in the oligomerization of the toxin and its insertion into the membrane, and
Domains II and III are made up of β-sheets and are involved in the attachment to
the receptors (Soberon et al. 2010).
The mechanism of toxicity begins when the insect ingests food contaminated
with spores; the crystal is then solubilized in the midgut, releasing protoxins which
are activated by proteases, and the toxic fragment binds in sequential fashion to
receptors located in the microvilli. The first receptor is a cadherin-like protein
(CADP), whose interaction allows the oligomerization of the toxin. This oligomeric
R.A. Grande-Cano
Unidad Universitaria de Secuenciaci
on Masiva de DNA-UNAM, Av. Universidad 2001,
Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
I. Gomez (*)
Departamento de Microbiologı́a Molecular, Instituto de Biotecnologı́a-UNAM,
Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico

DOI 10.1007/978-3-319-14499-3_4
72 R.A. Grande-Cano and I. G
omez
structure increases its affinity to the second receptor, either aminopeptidase-N

(APN) or alkaline phosphatase (ALP), anchored by glycosylphosphatidyl-inositol
(GPI) to the lipid raft membrane (Pacheco et al. 2009; Arenas et al. 2010). Finally,
the oligomer is inserted into the lipid raft, forming holes in the membrane, allowing
swelling and death of the intestinal cells (Zhuang et al. 2002; Bravo et al. 2004).
When the intestinal epithelium is destroyed, the spores are able to access the
hemolymph, a source rich in nutrients for its growth. Many species of insects are
susceptible; one of the main characteristics of Cry toxins is its specificity which is
determined by its recognition of the receptors, although the solubilization and
activation steps due to the physical and chemical environment of the midgut of
the insects are also important determining factors (Pigott and Ellar 2007;
Soberon et al. 2009; Bravo et al. 2013).
As mentioned previously, Cry toxins are highly selective and kill only a limited
number of insect species. This selectivity is mainly due to the interaction of Cry
toxins with larval proteins located in the midgut epithelial cells. The crucial role of
this receptor binding for toxicity is emphasized by the observation that insects
selected for resistance to a Cry toxin often have no or reduced binding capacity for
that toxin (Ferre and Van Rie 2002). A major research effort has taken place in the
identification of insect proteins that bind Cry toxins and mediate toxicity. Among
these, two major types of receptors have been identified: transmembrane proteins,
such as cadherins, and proteins anchored to the membrane such as the
GPI-anchored proteins that have been proposed to be involved in the action of
Cry toxins (Pigott and Ellar 2007).
After it was demonstrated that specific high-affinity toxin-binding sites are
present in the midgut of susceptible insects, efforts to identify and clone these
molecules have been intensified. Several putative Cry toxin receptors have since
been reported, of which the best characterized are the aminopeptidase N (APN)
receptors (Knight et al. 1994; Sangadala et al. 1994; Gill et al. 1995) and the
cadherin-like receptors (Vadlamudi et al. 1993; Nagamatsu et al. 1998; Gahan
et al. 2001; Chen et al. 2014) identified in lepidopterans. In nematodes, glycolipids
are believed to be an important class of Cry toxin receptors (Griffitts et al. 2005).
Other putative receptors include ALPs (Jurat-Fuentes and Adang 2004; Fernandez
et al. 2006; Arenas et al. 2010; Zuniga-Navarrete et al. 2013), a 270-kDa
glycoconjugate (Valaitis et al. 2001), and a 252-kDa protein (Hossain et al. 2004).
4.2 Toxin Receptor by Binding Blot Overlay
Radioligand binding assay remains the most sensitive quantitative technique to

measure binding parameters of affinity and receptor density and is widely used to
characterize receptors and determine their anatomical distribution. In saturation
experiments, tissue sections, cultured cells, or homogenates are incubated with an
increasing concentration of a radiolabeled ligand. Analysis using iterative nonlinear
curve-fitting programs, such as Scatchard, measures the affinity of the labeled
4 Identification and Characterization of Receptors for Insecticidal Toxins. . . 73
ligand for a receptor (equilibrium dissociation constant, KD), receptor density

(Bmax), and hill slope (nH). The affinity and selectivity of an unlabeled ligand to
compete for the binding of a fixed concentration of a radiolabeled ligand to a
receptor are determined using a competition-binding assay. Kinetic assays measure
the rate of association to or dissociation from a receptor from which a kinetic KD
may be derived (Maguire et al. 2012).
In principle, a blot overlay is similar to a Western blot. For both procedures,
samples are run on SDS–PAGE gels, transferred to nitrocellulose or PVDF, and
then overlaid with a Cry soluble protein that may bind to one or more immobilized
proteins on the blot. In the case of a Western blot, the overlaid protein is antibody.
In the case of a blot overlay, the overlaid protein is Cry toxin labeled with biotin or
radiolabeled usually with I125. The overlaid probe can be detected either via
incubation with an antibody (this method is often referred to as a “Far Western
blot”), via incubation with streptavidin (if the probe is biotinylated), or via auto-
radiography if the overlaid probe is radiolabeled.
4.2.1 Using One-Dimensional Electrophoresis
Toxin-binding proteins from several insects have been identified by ligand blot
analysis of brush border membrane (BBM) vesicles (BBMVs) prepared from the
midguts of the insects (Garczynski et al. 1991). The protein blot overlay assay is a
powerful technique for identifying proteins transferred to an immobilizing matrix.
It is based on the specific binding of labeled ligands with proteins of interest blotted
onto the membrane, followed by detection of the complexes formed.
Specific protein receptors involved in Cry toxin killing of target insects has been
known since the mid-1980s. Studies of the binding of radiolabeled Cry toxins in
suspensions of insect midgut proteins isolated by various procedures have gener-
ated a rather extensive list of putative receptor molecules (Hofmann et al. 1988;
Van Rie et al. 1989; Lee et al. 1992; Denolf et al. 1993; Escriche et al. 1997;
Simpson et al. 1997) without simultaneously identifying the binding protein(s) in
question by the use of midgut protein sodium dodecyl sulfate (SDS)–polyacryl-
amide gel electrophoresis (PAGE) blots. SDS–PAGE blots, when incubated with
radiolabeled toxins, at least permit a visual estimation of both the number of
proteins involved and their molecular masses. Ligand blots of Manduca sexta
midgut proteins have been used successfully to identify and partially characterize
CADP and aminopeptidase as insect binding proteins for the Cry1A lepidopteran-
specific toxins (Garczynski et al. 1991; Vadlamudi et al. 1993, 1995; Martinez-
Ramirez et al. 1994; de Maagd et al. 1996; Francis and Bulla 1997; Keeton and
Bulla 1997) and the polyphagous pest Heliothis virescens (Gill et al. 1995;
Luo et al. 1997; Oltean et al. 1999).
omez
4.2.2 Using Two-Dimensional Electrophoresis
Two-dimensional gel electrophoresis is a powerful and widely used method for the
analysis of complex protein mixtures extracted from cells, tissues, or other bio-
logical samples. This technique separates proteins in two steps, according to two
independent properties: the first dimension is isoelectric focusing (IEF), which
separates proteins according to their isoelectric points (pI); the second dimension
is SDS–PAGE, which separates proteins according to their molecular weights.
In this way, complex mixtures consisting of thousands of different proteins
can be resolved and the relative amount of each protein can be determined
(Rabilloud and Lelong 2011).
The insect midgut brush border has distinct structural elements; for this reason,
the identification of the proteome of BBMVs is a necessary step in defining
potential Cry toxin receptors. Because the insect midgut is the primary target site
for Bt toxins, several groups are focusing on constitutive expression changes in the
midgut proteins of resistant versus susceptible larvae. For that purpose, a compar-
ative analysis of BBM proteins using 2D DIGE (two-dimensional differential in-gel
electrophoresis) with mass spectrometry-based proteomic identification can result
in unique protein profiles and insight into functional processes of toxin–receptor
interactions. The goal of two-dimensional electrophoresis is to separate and display
all gene products present. As the only method currently available, which is capable
of simultaneously separating thousands of proteins, it has been very useful in the
identification of new putative receptors for Cry toxins.
Proteomic approaches have been previously used to identify novel Cry toxin-
binding proteins (McNall and Adang 2003) or compare proteomes from
Cry-susceptible and -resistant insects (Candas et al. 2003) or cell lines (Liu
et al. 2004). In these studies, the increased resolving power of 2D electrophoresis
allowed for identification of proteins that were not successfully resolved using
traditional SDS–PAGE electrophoresis. One of the most interesting proteins con-
firmed with this kind of methodology is the ALP, involved in Cry1Ac binding with
H. virescens midgut (Jurat-Fuentes and Adang 2007). Many other proteins have
been detected by 2D electrophoresis but they need to be confirmed in vivo by other
strategies (McNall and Adang 2003; Krishnamoorthy et al. 2007; Bayyareddy
et al. 2009; Gai et al. 2013).
4.3 Toxin-Affinity Chromatography
This technique involves the elution of a single protein from an affinity column after
prior elution of nonspecifically adsorbed proteins. Cry toxins are coupled to
Sepharose (an insoluble, large, pore-sized chromatographic matrix). The proteins
from the midgut are incubated and bind to the Cry toxins covalently bound to the
matrix. To elute the bound molecules from the affinity matrix, the toxin–receptor
interaction is destabilized by incubation with SDS solubilization buffer according

to the procedure of Laemmli (Laemmli 1970). After electrophoresis, eluted proteins
can be recovered by electro-elution to sequence. Several receptors for different Cry
toxins have been identified with this methodology. Cadherin from M. sexta was
isolated using immobilized Cry1Ab toxin (Vadlamudi et al. 1993); also amino-
peptidase was identified in combination with overlay assay with Cry1Ac toxin
(Knight et al. 1994) and with Cry1Ab toxin (Denolf et al. 1993). More recently, a
similar strategy was used to identify an ALP as receptor for Cry11A toxin in Aedes
aegypti mosquito (Fernandez et al. 2006).
4.4 Immunohistochemical Detection of Toxin-Binding

Receptors
In situ screening of histological sections could reveal the cell-specific distribution

of Cry toxin receptors in target tissues using histochemical or immunohisto-
chemical staining. Because of the superior morphology provided by formalin-
fixed paraffin-embedded tissues, this has become the medium of choice for most
research studies with the Cry toxins. The peroxidase-labeled antibody method,
introduced in 1968, was the first practical application of antibodies to paraffin-
embedded tissues and overcame some of the limitations of earlier fluorescence
antibody methods (Nakane 1968). These pioneering studies using enzyme labels
instead of fluorescent dyes opened the door to the development of modern methods
of immunohistochemistry. In 1981 a new generation of immunohistochemical
methods emerged with the advent of the avidin–biotin methods, which remains
widely used today (Hsu et al. 1981). All avidin–biotin methods rely on the strong
affinity of avidin or streptavidin for the vitamin biotin.
The cytology and ultrastructure of the midgut cells of M. sexta and other
lepidopteran larvae was reported in the 1980s (Endo and Nishiitsutjiuwo 1980).
This made it easier to study the toxic effects of Bt toxins on the insect midgut. After
intoxication with spore preparations from Bt, it was observed that the epithelial
cells of the midgut swell shortly after ingestion of the Bt toxins. Eventually,
the cells burst and release their cytoplasmatic content into the midgut lumen
(Lane et al. 1989).
Immunocytochemical localization of toxin-fed larvae has demonstrated that the
insecticidal proteins accumulate in the microvilli of the gut epithelial cells (Rausell
et al. 2000). Histopathological studies have shown that one of the most rapid effects
of Bt Cry intoxication is a striking change in the microvilli targeted by the Bt Cry
toxins. A reduction of binding sites is a hallmark of development of resistance to Bt,
because toxicity depends on the availability of specific high-affinity receptors.
Receptor expression levels have been shown to correlate with Cry toxin activity.
For example, in M. sexta, the three identified protein receptors are expressed in the
anterior, middle, and posterior regions of the midgut (Chen et al. 2005).
omez
4.5 Expression of Receptors in Cell Lines
Insect cell lines have been used to study Cry toxin membrane insertion and channel
formation (Vachon et al. 1995). Cry toxins cause a cytotoxic response in some
insect cell lines, and frequently cell swelling served as a marker of cytotoxicity
(Pigott and Ellar 2007). Various types of insect cell lines have also been studied for
their toxicity response to different Cry toxins (Johnson 1994), and it was found that
Sf-9 cells showed very low toxicity to Cry1Ab and showed maximum toxicity with
Cry1C (Kwa et al. 1998). Cell lines not susceptible to Cry1 toxins can be used to
test the receptor function in vivo. The effectiveness of using live insect cells
expressing putative receptor proteins and demonstrating the ability of the protein
to function as a receptor to Bt toxin has been shown by Nagamatsu et al. (1999).
They have been able to demonstrate swelling and lysis of Sf-9 cells expressing
cadherin protein of Bombyx mori after overlay with Cry1Ab toxin. Purified mem-
branes from COS cells expressing Bt-R1 (cadherin from M. sexta) bound all three
Cry1A toxins in binding assays and ligand blots (Keeton and Bulla 1997). Further-
more, expression of Bt-R1 on the surface of COS7 cells led to toxin-induced cell
toxicity as monitored by immunofluorescence microscopy with fixed cells (Dorsch
et al. 2002). Drosophila S-2 cell line constitutively expressing M. sexta cadherin
gene showed heavy damage and lysis when overlaid with Cry1Ab (Hua et al. 2004).
4.6 RNAi
RNA interference has become an effective and important tool to study the func-
tional relevance of various proteins and genes in an organism. As described in detail
in various reviews, RNAi by dsRNA results in sequence-specific posttranscriptional
degradation of the target mRNA (Agrawal et al. 2003). The principle behind the use
of RNAi technology is that entry of exogenous dsRNA into a cell can activate an
innate defense process that results into the breakdown of the dsRNA and the
degradation of any endogenous RNA molecule having the same nucleotide
sequence. Therefore, RNAi provides a means of protection for eukaryotic cells
against aberrant RNAs. The dsRNA thus induces a sequence-specific silencing of
gene expression at the posttranscriptional level (Hakim et al. 2010). This property
has led to the generation and analysis of the phenotypic effect(s) caused by targeted
knockdown of specific mRNAs. In the past few years, several studies have explored
the potential of RNAi as an innovative, promising strategy for controlling a number
of agriculturally important insect pests (Baum et al. 2007; Gordon and Waterhouse
2007).
The success of oral RNAi in vivo is determined primarily by the presence of an
uptake mechanism for dsRNA in the target cells, but little is known about the
mechanism of dsRNA uptake by the insect midgut and its further spread throughout
the insect body (Hakim et al. 2010).
Successful RNAi experiments have been carried out in a number of lepidopteran

species to date (Terenius et al. 2011). The first lepidopteran RNAi publications
appeared in 2002; one reported the knockdown of a pigment gene following dsRNA
injection into B. mori embryos (Quan et al. 2002); another targeted a pattern
recognition protein, hemolin, in Hyalophora cecropia embryos by heritable RNAi
(Bettencourt et al. 2002); and a third targeted a putative B. thuringiensis toxin
receptor in Spodoptera litura larvae (Rajagopal et al. 2002).
Feeding of dsRNA is an even more attractive approach than hemocoel injection
because it is noninvasive. Interest for this approach received a great boost after the
high-profile publications of its feasibility in several pest insect species, including
the lepidopteran H. armigera (Baum et al. 2007; Mao et al. 2007). Feeding of
dsRNA has been applied with greatest effect in Plutella xylostella, S. exigua,
M. sexta, and Ostrinia nubilalis (Bautista et al. 2009; Tian et al. 2009; Whyard
et al. 2009; Yang et al. 2010; Flores-Escobar et al. 2013). Some targets successfully
blocked by oral delivery of dsRNA are involved in the mode of action of Cry toxins,
such as aminopeptidase in H. armigera, S. litura, S. exigua, and M. sexta, cadherin
in S. exigua and M. sexta, and ALP in M. sexta (Rajagopal et al. 2002; Sivakumar
et al. 2007; Flores-Escobar et al. 2013; Park and Kim 2013; Ren et al. 2013, 2014).
4.7 Transcriptome as Tool to Identify New Proteins

Involved in the Mechanism of Action of Cry Toxins
RNA-Seq is a set of methodologies that allows us to analyze the transcriptome of a

tissue or set of cells in a given time and under specific growth conditions, using the
technologies of next-generation massive sequencing (Mutz et al. 2013). The term
transcriptome was coined nearly two decades ago by Charles Auffray to define the
set of transcripts (mRNA, small RNA, lncRNA, rRNA, etc.) into a set of cells or
tissues (McGettigan 2013). Historically, large-scale transcriptome studies were
based mainly on microarray analysis and supported in many cases by RT-PCR
experiments. The first report for monitoring the expression of genes in parallel
using a microarray as we know today was described by Schena et al. (1995).
Technical limitations of this technology relative to RNA-Seq studies have enabled
the latter to develop at an incredible speed. For example, to perform microarray
studies, it is necessary to know the sequence of the organism to design the
microarray. In comparison, studies of RNA-Seq do not require prior knowledge
of the genome of the organism to perform the experiment. In addition, due to the
large number of related sequences in organisms, microarray studies have been a
problem because of the cross hybridization between these sequences. A further
advantage is the high resolution of up to one base pair in RNA-Seq experiments
which allows to know in great detail and with high confidence the 50 and 30 ends of
the transcripts, which are unable with studies with microarrays. This last point is
important because it allows us to know with high accuracy the regulatory regions
omez
governing expression of a gene. A fundamental disadvantage of microarray studies

upon RNA-Seq studies is that due to the nature of the analogic signal, it is difficult
to quantify the high and low species of the RNA expressed. The wide dynamic
range of RNA-Seq experiments also allow us to determine better the differential
gene expression (Nagalakshmi et al. 2008) compared with microarray experiments,
since the latter have a more limited dynamic range. Experiments of RNA-Seq have
also allowed the identification of new forms of alternative splicing, results that
would be impossible to obtain via microarray experiments since the latter can tell us
what is being expressed in a given time but not the structure of that transcript.
Finally, there is a high reproducibility of results between studies of RNA-Seq in
biological and technical replicates compared to microarray-based studies.
RNA-Seq analysis begins with the preparation of a suitable sample of total RNA.
To this end, currently there are a large number of commercially available kits that
help us to obtain high-quality RNA from limited quantities of biological samples
and even to obtain different RNA species from the same preparation. Once the RNA
has been purified, an evaluation of the integrity of the material is necessary to
proceed with the construction libraries. The sample analysis is performed through
the use of devices like 2100 Bioanalyzer or 2200 TapeStation from Agilent that
allow us to analyze quickly and accurately the concentration and integrity of the
RNA samples, the latter via RNA integrity number (RIN) calculation (Schroeder
et al. 2006). After obtaining an adequate sample of RNA, the next step is the
construction of the library. To this end, several methods have been developed that
allow us to build general or strand-specific libraries from a specific amount of
genetic material (Levin et al. 2010).
Some of the studies of differential gene expression in insects using massive
sequencing technologies have been made using the 454 platform of Roche (Park
et al. 2009; Pauchet et al. 2010; Oppert et al. 2012); however, even when the reads
are large in average size which facilitates the assembly and subsequent analysis,
they are relatively few compared to those obtained from massive sequencing
experiments using other platforms such as Illumina platforms Genome Analyzer
GAIIx and HiSeq (He et al. 2012; Ma et al. 2012; Lei et al. 2014). The sequence
data obtained should be analyzed using a limited number of programs currently
available on the Internet. Most of these programs are specific to running in Linux
environments, which require adequate computer infrastructure. When a reference
genome is available, the data can be aligned with the help of programs like Bowtie
(Langmead et al. 2009), SOAP (Li et al. 2008) or BWA (Li and Durbin 2009).
Meanwhile, when a reference genome is not available, the data can be assembled de
novo using SOAPdenovo (Li et al. 2009), SMART, or Trinity (Grabherr
et al. 2011), among several others (Garber et al. 2011).
With the development of the new technologies for massive sequencing
(Margulies et al. 2005; Bentley et al. 2008; Rothberg et al. 2011), RNA-Seq studies
have become extremely important, especially for those non-model organisms where
no genomic information is available for the transcriptome analysis. Currently, many
pests of economically important crops are managed using chemical insecticides
with high efficiency. However, the indiscriminate use of these products brings
many consequences at different levels, from potential hazards to the environment

and food to the development of resistance by organisms against many of the
chemicals used in the manufacture of insecticides. By this reason, the pest manage-
ment using biological control is being increasingly used.
Even when the mechanism of action of Cry toxins and their interaction with their
receptors in some insect models is known in detail, the absence of genomic
information in most of them has delayed further study, i.e., to understand in a
better way the mechanism and thereby applying in a more suitable way the
biological mechanism to control pests attacking economically important crops.
For this reason, studies focused on the generation of genomic information of
organisms affecting crop plants are required.
In an analysis of RNA-Seq complemented with microarray experiments (using
the 454 platform of Roche) performed on Tenebrio molitor, a stored grain pest,
intoxicated with toxin Cry3Aa of Bt, Oppert et al. (2012) found that the expression
of the three best characterized receptors in insects to Bt toxin, cadherin,
aminopeptidase N, and ALP, occurs in a very particular way. No differences
were observed in the expression pattern of the cadherin and aminopeptidase N
receptors amongst the control larvae and larvae intoxicated with the Cry3Aa after
24 h of intoxication. However, transcripts associated with the enzyme ALP, also
proposed to be acting as a receptor to the Cry1Ac toxin in Lepidoptera, were
observed only in the larvae intoxicated with the Cry3Aa toxin. Interestingly,
increased levels of ALP were observed in Cry1Ab-resistant European corn borer,
O. nubilalis (Khajuria et al. 2009). In a subsequent analysis using a custom script
for function assignment, enrichment for biological processes was obtained, specifi-
cally an enrichment of genes associated with transport, suggesting that once the
larva is intoxicated it tries to maintain ionic homeostasis (Oppert et al. 2012).
The diamondback moth (P. xylostella) is a widely distributed insect that belongs
to the order Lepidoptera. Currently, it has great economic importance because this
insect is a plague of cruciferous vegetables like cabbage, broccoli, and mainly
cauliflower, important crops for human consumption. With the indiscriminate use
of synthetic insecticides, the diamondback moth became the first pest to develop
resistance to DDT; in addition, this was also the first insect to develop resistance in
the field to B. thuringiensis bacteria (Talekar and Shelton 1993). In a transcriptome
analysis of the midgut of two resistant strains of P. xylostella and one sensitive to
Cry1Ac toxin, Lei et al. found that when the insects are challenged with Cry1Ac
toxin, expression of 34 sequences related to ABC transporters, two unigenes related
to aminopeptidase, and four related to cadherin, all of them possibly associated to
the resistance to Cry1Ac was observed. Fifteen sequences out of 34 related to ABC
transporters were common to both strains resistant to Cry1Ac. In addition, six
differentially expressed unigenes (DEU) coding to aminopeptidase and cadherin
in the resistant strains of P. xylostella had differential expression levels of 2–10-
fold compared to susceptible strain (Lei et al. 2014). In a previous transcriptome
analysis using the Genome Analyzer IIx (GAIIx) of Illumina and Sanger sequenc-
ing, He et al. found that genes coding for CADP, aminopeptidase N, and intestinal
mucin were the three most abundant transcripts related to resistance to Bt in
omez
diamondback moth in four different stages of development and two insecticide-

resistant strains (He et al. 2012).
Leptinotarsa decemlineata also known as the Colorado potato beetle is an insect
native to Mexico and the Southeastern USA. This insect is an important plague of
potato because both larvae and adult organisms feed on potato leaves and several
other related species in the Solanaceae family as Solanum rostratum and
S. angustifolium, causing drastic damage to potato yields. Even though
L. decemlineata was exposed to S. tuberosum since 1820, the change to potato
feed was documented until 1859 in the USA (Casagrande 1987). The pest was
accidentally established in France in 1920, from where it had spread through
Europe, becoming a problem in Asia Minor, Iran, Central Asia, and western
China at the end of 20th century (Grapputo et al. 2005). Previous studies have
shown that the ADAM metalloprotease serves as a receptor for the Cry3Aa toxin in
L. decemlineata (Ochoa-Campuzano et al. 2007). Additionally, the Cry3Aa toxin
also binds to calmodulin in a calcium-independent manner (Ochoa-Campuzano
et al. 2012) and to cadherin fragments (Park et al. 2009). By using a pyrosequencing
strategy on the 454-FLX platform of Roche, Kumar et al. identified 621 contigs
involved in insecticide resistance; of these three contigs belonged to metallo-
protease ADAM, 10 to cadherin, and 98 to calmodulin, among others (Kumar
et al. 2014).
Bt var. israelensis (Bti) produces a set of proteins during sporulation, which are
toxic against the mosquito A. aegypti. In an analysis of BBM proteins using 2D
DIGE and microarray transcriptome analysis supplemented with RT-PCR reactions
against Bti toxin receptors, ALP, cadherin, and aminopeptidase, of an A. aegypti
strain resistant to Bti Cry toxins which was obtained in the laboratory after
30 generations of selection with leaf litter containing Bti and a susceptible strain,
Tetreau et al. found that even though they detected an altered expression of pro-
teases and Cry toxin receptor proteins by the two methods used, very little
overlapping results were found. The reason for this may be due to technical
limitations inherent in each method. It is expected that the repertoire of proteins
anchored to the membrane, those obtained for the assay of 2D DIGE, might be very
different to that coded in transcriptome assay (Tetreau et al. 2012).
The rice borer worm (Chilo suppressalis) is an insect belonging to the order
Lepidopteran and is an important pest for this cereal. Although rice is a staple crop
for many countries in Asia, very little genomic information is available on this pest.
In an analysis of midgut transcriptome using the Illumina Genome Analyzer II
platform complemented by an analysis of proteome, Ma et al. generated a total of
37,040 contigs from 39 million Illumina reads. From the total assembled contigs
obtained, they were able to identify 16 for cadherin-like transcripts, 27 for APNs,
and 11 for ALPs. This correlates with proteome analysis, specifically by Western
blot analysis against the proteins that bind to Cry1Ac. Using this technique, they
were able to detect two isoforms of APN but were unable to detect cadherin
probably due to limitations of the technique to resolve proteins with high molecular
weight (Ma et al. 2012).
M. sexta is a moth that belongs to the order Lepidoptera; defoliators of tobacco,

tomato, and other plants of the Solanaceae family characterize this group of insects
and are present over much of the American continent. Because of its large size,
M. sexta has served as a model organism to study the mechanism of action of Cry
toxins from Bt (Shields and Hildebrand 2001; Kanost et al. 2004; Pigott and Ellar
2007). Even for this important model organism, there was very little genetic
information available before 2008, when Zou et al. through a transcriptome analysis
using the first generation of pyrosequencing technology of 454 identified a large
number of ESTs associated with hemolymph and immune system which consider-
ably increased the genomic information available for this organism (Zou
et al. 2008). Further analysis using the second-generation technology of 454 mas-
sive sequencing device and conventional Sanger sequencing, Pauchet
et al. identified over 387,000 ESTs from the M. sexta midgut transcriptome.
Functional analysis of these data allowed to group contigs in functions related to
digestion, detoxification, Bt toxin binding, peritrophic membrane metabolism, and
innate immunity. Among the candidates for Bt toxin binding, 19 contigs were
identified as coding for APNs, of which 12 were previously known and 7 genes
coding for completely new APNs (Pauchet et al. 2010).
Cry toxins are highly selective against their target insect; they present a complex
mechanism that involves interaction with several receptors. However, insects may
become resistant to these proteins and alternatives to counteract this potential
problem must be generated soon. The cellular distribution of receptors in the target
tissues and its identification and characterization are very important in the study of
the mode of action of Cry toxins produced by B. thuringiensis. Several in vitro and
in vivo methodologies have been used with interesting results. The combination
with the new era of genomics and proteomics promises great advances in the
identification of functional proteins as receptors. This information will be useful
to design new strategies for pest control.
Acknowledgments Our thanks to PAPIIT/UNAM IN213514 for the support in our

research work.
References
Agrawal N, Dasaradhi PV, Mohmmed A, Malhotra P, Bhatnagar RK, Mukherjee SK (2003) RNA
interference: biology, mechanism, and applications. Microbiol Mol Biol Rev 67:657–685
Arenas I, Bravo A, Soberon M, Gomez I (2010) Role of alkaline phosphatase from Manduca sexta
in the mechanism of action of Bacillus thuringiensis Cry1Ab toxin. J Biol Chem 285:
12497–12503
omez
Baum JA, Bogaert T, Clinton W, Heck GR, Feldmann P, Ilagan O, Johnson S, Plaetinck G,
Munyikwa T, Pleau M, Vaughn T, Roberts J (2007) Control of coleopteran insect pests through
RNA interference. Nat Biotechnol 25:1322–1326
Bautista MA, Miyata T, Miura K, Tanaka T (2009) RNA interference-mediated knockdown of a
cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval
resistance to permethrin. Insect Biochem Mol Biol 39:38–46
Bayyareddy K, Andacht TM, Abdullah MA, Adang MJ (2009) Proteomic identification of Bacillus
thuringiensis subsp. israelensis toxin Cry4Ba binding proteins in midgut membranes from
Aedes (Stegomyia) aegypti Linnaeus (Diptera, Culicidae) larvae. Insect Biochem Mol Biol 39:
279–286
Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J, Brown CG, Hall KP,
Evers DJ, Barnes CL, Bignell HR, Boutell JM, Bryant J, Carter RJ, Cheetham RK, Cox AJ,
Ellis DJ, Flatbush MR, Gormley NA, Humphray SJ, Irving LJ, Karbelashvili MS, Kirk SM,
Li H, Liu X, Maisinger KS, Murray LJ, Obradovic B, Ost T, Parkinson ML, Pratt MR,
Rasolonjatovo IM, Reed MT, Rigatti R, Rodighiero C, Ross MT, Sabot A, Sankar SV,
Scally A, Schroth GP, Smith ME, Smith VP, Spiridou A, Torrance PE, Tzonev SS,
Vermaas EH, Walter K, Wu X, Zhang L, Alam MD, Anastasi C, Aniebo IC, Bailey DM,
Bancarz IR, Banerjee S, Barbour SG, Baybayan PA, Benoit VA, Benson KF, Bevis C, Black
PJ, Boodhun A, Brennan JS, Bridgham JA, Brown RC, Brown AA, Buermann DH, Bundu AA,
Burrows JC, Carter NP, Castillo N, Chiara ECM, Chang S, Neil Cooley R, Crake NR,
Dada OO, Diakoumakos KD, Dominguez-Fernandez B, Earnshaw DJ, Egbujor UC,
Elmore DW, Etchin SS, Ewan MR, Fedurco M, Fraser LJ, Fuentes Fajardo KV,
Scott Furey W, George D, Gietzen KJ, Goddard CP, Golda GS, Granieri PA, Green DE,
Gustafson DL, Hansen NF, Harnish K, Haudenschild CD, Heyer NI, Hims MM, Ho JT,
Horgan AM, Hoschler K, Hurwitz S, Ivanov DV, Johnson MQ, James T, Huw Jones TA,
Kang GD, Kerelska TH, Kersey AD, Khrebtukova I, Kindwall AP, Kingsbury Z, Kokko-
Gonzales PI, Kumar A, Laurent MA, Lawley CT, Lee SE, Lee X, Liao AK, Loch JA, Lok M,
Luo S, Mammen RM, Martin JW, McCauley PG, McNitt P, Mehta P, Moon KW, Mullens JW,
Newington T, Ning Z, Ling Ng B, Novo SM, O’Neill MJ, Osborne MA, Osnowski A,
Ostadan O, Paraschos LL, Pickering L, Pike AC, Chris Pinkard D, Pliskin DP, Podhasky J,
Quijano VJ, Raczy C, Rae VH, Rawlings SR, Chiva Rodriguez A, Roe PM, Rogers J,
Rogert Bacigalupo MC, Romanov N, Romieu A, Roth RK, Rourke NJ, Ruediger ST,
Rusman E, Sanches-Kuiper RM, Schenker MR, Seoane JM, Shaw RJ, Shiver MK,
Short SW, Sizto NL, Sluis JP, Smith MA, Ernest Sohna Sohna J, Spence EJ, Stevens K,
Sutton N, Szajkowski L, Tregidgo CL, Turcatti G, Vandevondele S, Verhovsky Y, Virk SM,
Wakelin S, Walcott GC, Wang J,Worsley GJ, Yan J, Yau L, Zuerlein M, Rogers J, Mullikin JC,
Hurles ME, McCooke NJ, West JS, Oaks FL, Lundberg PL, Klenerman D, Durbin R, Smith AJ
(2008) Accurate whole human genome sequencing using reversible terminator chemistry.
Nature 456:53–59
Bettencourt RO, Terenius O, Faye I (2002) Hemolin gene silencing by ds-RNA injected into
Cecropia pupae is lethal to next generation embryos. Insect Mol Biol 11:267–271
Bravo A, Gomez I, Conde J, Munoz-Garay C, Sanchez J, Miranda R, Zhuang M, Gill SS,
Soberon M (2004) Oligomerization triggers binding of a Bacillus thuringiensis Cry1Ab
pore-forming toxin to aminopeptidase N receptor leading to insertion into membrane
microdomains. Biochim Biophys Acta 1667:38–46
Bravo A, Likitvivatanavong S, Gill SS, Soberon M (2011) Bacillus thuringiensis: a story of a
successful bioinsecticide. Insect Biochem Mol Biol 41:423–431
Bravo A, Gomez I, Porta H, Garcia-Gomez BI, Rodriguez-Almazan C, Pardo L, Soberon M (2013)
Evolution of Bacillus thuringiensis Cry toxins insecticidal activity. Microb Biotechnol 6:
17–26
Candas M, Loseva O, Oppert B, Kosaraju P, Bulla LA Jr (2003) Insect resistance to Bacillus
thuringiensis: alterations in the indianmeal moth larval gut proteome. Mol Cell Proteomics 2:
19–28
Casagrande RA (1987) The Colorado potato beetle: 125 years of mismanagement. Bull ESA
33:142–150
Chen J, Brown MR, Hua G, Adang MJ (2005) Comparison of the localization of Bacillus
thuringiensis Cry1A delta-endotoxins and their binding proteins in larval midgut of tobacco
hornworm, Manduca sexta. Cell Tissue Res 321:123–129
Chen RR, Ren XL, Han ZJ, Mu LL, Li GQ, Ma Y, Cui JJ (2014) A cadherin-like protein from the
beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) is a putative Cry1Ac receptor.
Arch Insect Biochem Physiol 86:58–71
de Maagd RA, van der Klei H, Bakker PL, Stiekema WJ, Bosch D (1996) Different domains of
Bacillus thuringiensis delta-endotoxins can bind to insect midgut membrane proteins on ligand
blots. Appl Environ Microbiol 62:2753–2757
Denolf P, Jansens S, Peferoen M, Degheele D, Van Rie J (1993) Two different Bacillus
thuringiensis delta-endotoxin receptors in the midgut brush border membrane of the
European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Pyralidae). Appl Environ
Dorsch JA, Candas M, Griko NB, Maaty WS, Midboe EG, Vadlamudi RK, Bulla LA Jr (2002)
Cry1A toxins of Bacillus thuringiensis bind specifically to a region adjacent to the membrane-
proximal extracellular domain of BT-R(1) in Manduca sexta: involvement of a cadherin in the
entomopathogenicity of Bacillus thuringiensis. Insect Biochem Mol Biol 32:1025–1036
Endo Y, Nishiitsutjiuwo J (1980) Mode of action of Bacillus thuringiensis δ-endotoxin. Histo-
pathological changes in the silkworm. J Invertebr Pathol 36:90–103
Escriche B, Ferre J, Silva FJ (1997) Occurrence of a common binding site in Mamestra brassicae,
Phthorimaea operculella, and Spodoptera exigua for the insecticidal crystal proteins CryIA
from Bacillus thuringiensis. Insect Biochem Mol Biol 27:651–656
Fernandez LE, Aimanova KG, Gill SS, Bravo A, Soberon M (2006) A GPI-anchored alkaline
phosphatase is a functional midgut receptor of Cry11Aa toxin in Aedes aegypti larvae.
Biochem J 394:77–84
Ferre J, Van Rie J (2002) Biochemistry and genetics of insect resistance to Bacillus thuringiensis.
Flores-Escobar B, Rodriguez-Magadan H, Bravo A, Soberon M, Gomez I (2013) Differential role
of Manduca sexta aminopeptidase-N and alkaline phosphatase in the mode of action of
Cry1Aa, Cry1Ab, and Cry1Ac toxins from Bacillus thuringiensis. Appl Environ Microbiol
79:4543–4550
Francis BR, Bulla LA Jr (1997) Further characterization of BT-R1, the cadherin-like receptor for
Cry1Ab toxin in tobacco hornworm (Manduca sexta) midguts. Insect Biochem Mol Biol 27:
541–550
Gahan LJ, Gould F, Heckel DG (2001) Identification of a gene associated with Bt resistance in
Heliothis virescens. Science 293:857–860
Gai Z, Zhang X, Wang X, Peng J, Li Y, Liu K, Hong H (2013) Differential proteomic analysis of
Trichoplusia ni cells after continuous selection with activated Cry1Ac toxin. Cytotechnology
65:425–435
Garber M, Grabherr MG, Guttman M, Trapnell C (2011) Computational methods for
transcriptome annotation and quantification using RNA-seq. Nat Methods 8:469–477
Garczynski SF, Crim JW, Adang MJ (1991) Identification of putative insect brush border
membrane-binding molecules specific to Bacillus thuringiensis delta-endotoxin by protein
blot analysis. Appl Environ Microbiol 57:2816–2820
Gill SS, Cowles EA, Francis V (1995) Identification, isolation, and cloning of a Bacillus
thuringiensis CryIAc toxin-binding protein from the midgut of the lepidopteran insect
Heliothis virescens. J Biol Chem 270:27277–27282
Gordon KH, Waterhouse PM (2007) RNAi for insect-proof plants. Nat Biotechnol 25:1231–1232
Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L,
Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F,
Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A (2011) Full-length
omez
transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29:
644–652
Grapputo A, Boman S, Lindstrom L, Lyytinen A, Mappes J (2005) The voyage of an invasive
species across continents: genetic diversity of North American and European Colorado potato
beetle populations. Mol Ecol 14:4207–4219
Griffitts JS, Haslam SM, Yang T, Garczynski SF, Mulloy B, Morris H, Cremer PS, Dell A,
Adang MJ, Aroian RV (2005) Glycolipids as receptors for Bacillus thuringiensis crystal toxin.
Science 307:922–925
Hakim RS, Baldwin K, Smagghe G (2010) Regulation of midgut growth, development, and
metamorphosis. Annu Rev Entomol 55:593–608
He W, You M, Vasseur L, Yang G, Xie M, Cui K, Bai J, Liu C, Li X, Xu X, Huang S (2012)
Developmental and insecticide-resistant insights from the de novo assembled transcriptome of
the diamondback moth, Plutella xylostella. Genomics 99:169–177
Hofmann C, Vanderbruggen H, Hofte H, Van Rie J, Jansens S, Van Mellaert H (1988) Specificity of
Bacillus thuringiensis delta-endotoxins is correlated with the presence of high-affinity binding
sites in the brush border membrane of target insect midguts. Proc Natl Acad Sci U S A 85:
7844–7848
Hossain DM, Shitomi Y, Moriyama K, Higuchi M, Hayakawa T, Mitsui T, Sato R, Hori H (2004)
Characterization of a novel plasma membrane protein, expressed in the midgut epithelia of
Bombyx mori, that binds to Cry1A toxins. Appl Environ Microbiol 70:4604–4612
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in
immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP)
procedures. J Histochem Cytochem 29:577–580
Hua G, Jurat-Fuentes JL, Adang MJ (2004) Bt-R1a extracellular cadherin repeat 12 mediates
Bacillus thuringiensis Cry1Ab binding and cytotoxicity. J Biol Chem 279:28051–28056
Johnson DE (1994) Cellular toxicities and membrane binding characteristics of insecticidal crystal
proteins from Bacillus thuringiensis toward cultured insect cells. J Invertebr Pathol 63:
123–129
Jurat-Fuentes JL, Adang MJ (2004) Characterization of a Cry1Ac-receptor alkaline phosphatase in
susceptible and resistant Heliothis virescens larvae. Eur J Biochem 271:3127–3135
Jurat-Fuentes JL, Adang MJ (2007) A proteomic approach to study Cry1Ac binding proteins and
their alterations in resistant Heliothis virescens larvae. J Invertebr Pathol 95:187–191
Kanost MR, Jiang H, Yu XQ (2004) Innate immune responses of a lepidopteran insect,
Manduca sexta. Immunol Rev 198:97–105
Keeton TP, Bulla LA Jr (1997) Ligand specificity and affinity of BT-R1, the Bacillus thuringiensis
Cry1A toxin receptor from Manduca sexta, expressed in mammalian and insect cell cultures.
Appl Environ Microbiol 63:3419–3425
Khajuria C, Zhu YC, Chen MS, Buschman LL, Higgins RA, Yao J, Crespo AL, Siegfried BD,
Muthukrishnan S, Zhu KY (2009) Expressed sequence tags from larval gut of the European
corn borer (Ostrinia nubilalis): exploring candidate genes potentially involved in Bacillus
thuringiensis toxicity and resistance. BMC Genomics 10:286
Knight PJ, Crickmore N, Ellar DJ (1994) The receptor for Bacillus thuringiensis CrylA(c) delta-
endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase
N. Mol Microbiol 11:429–436
Krishnamoorthy M, Jurat-Fuentes JL, McNall RJ, Andacht T, Adang MJ (2007) Identification of
novel Cry1Ac binding proteins in midgut membranes from Heliothis virescens using proteomic
analyses. Insect Biochem Mol Biol 37:189–201
Kumar A, Congiu L, Lindstrom L, Piiroinen S, Vidotto M, Grapputo A (2014) Sequencing,
de novo assembly and annotation of the Colorado Potato Beetle, Leptinotarsa decemlineata,
transcriptome. PLoS One 9:e86012
Kwa MS, de Maagd RA, Stiekema WJ, Vlak JM, Bosch D (1998) Toxicity and binding properties
of the Bacillus thuringiensis delta-endotoxin Cry1C to cultured insect cells. J Invertebr Pathol
71:121–127
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacterio-
phage T4. Nature 227:680–685
Lane NJ, Harrison JB, Lee WM (1989) Changes in microvilli and Golgi-associated membranes of
lepidopteran cells induced by an insecticidally active bacterial δ-endotoxin. J Cell Sci 93:
337–347
Langmead B, Trapnell C, Pop M, Salzberg SL (2009) Ultrafast and memory-efficient alignment of
short DNA sequences to the human genome. Genome Biol 10:R25
Lee MK, Milne RE, Ge AZ, Dean DH (1992) Location of a Bombyx mori receptor binding region
on a Bacillus thuringiensis delta-endotoxin. J Biol Chem 267:3115–3121
Lei Y, Zhu X, Xie W, Wu Q, Wang S, Guo Z, Xu B, Li X, Zhou X, Zhang Y (2014) Midgut
transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidop-
tera: Plutellidae). Gene 533(1):180–187
Levin JZ, Yassour M, Adiconis X, Nusbaum C, Thompson DA, Friedman N, Gnirke A, Regev A
(2010) Comprehensive comparative analysis of strand-specific RNA sequencing methods.
Nat Methods 7:709–715
Li H, Durbin R (2009) Fast and accurate short read alignment with Burrows–Wheeler transform.
Bioinformatics 25:1754–1760
Li R, Li Y, Kristiansen K, Wang J (2008) SOAP: short oligonucleotide alignment program.
Bioinformatics 24:713–714
Li R, Yu C, Li Y, Lam TW, Yiu SM, Kristiansen K, Wang J (2009) SOAP2: an improved ultrafast
tool for short read alignment. Bioinformatics 25:1966–1967
Liu K, Zheng B, Hong H, Jiang C, Peng R, Peng J, Yu Z, Zheng J, Yang H (2004) Characterization
of cultured insect cells selected by Bacillus thuringiensis crystal toxin. In Vitro Cell Dev Biol
Anim 40:312–317
Luo K, Sangadala S, Masson L, Mazza A, Brousseau R, Adang J (1997) The Heliothis virescens
170 kDa aminopeptidase functions as “receptor A” by mediating specific Bacillus thuringiensis
Cry1A delta-endotoxin binding and pore formation. Insect Biochem Mol Biol 27:735–743
Ma W, Zhang Z, Peng C, Wang X, Li F, Lin Y (2012) Exploring the midgut transcriptome and
brush border membrane vesicle proteome of the rice stem borer, Chilo suppressalis (Walker).
PLoS One 7:e38151
Maguire JJ, Kuc RE, Davenport AP (2012) Radioligand binding assays and their analysis.
Methods Mol Biol 897:31–77
Mao YB, Cai WJ, Wang JW, Hong GJ, Tao XY, Wang LJ, Huang YP, Chen XY (2007) Silencing a
cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance
of gossypol. Nat Biotechnol 25:1307–1313
Margulies MM, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman
MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W,
Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight
JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB,
McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT,
Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA,
Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM (2005) Genome
sequencing in microfabricated high-density picolitre reactors. Nature 437:376–380
Martinez-Ramirez AC, Gonzalez-Nebauer S, Escriche B, Real MD (1994) Ligand blot identifi-
cation of a Manduca sexta midgut binding protein specific to three Bacillus thuringiensis
CryIA-type ICPs. Biochem Biophys Res Commun 201:782–787
McGettigan PA (2013) Transcriptomics in the RNA-seq era. Curr Opin Chem Biol 17:4–11
McNall RJ, Adang MJ (2003) Identification of novel Bacillus thuringiensis Cry1Ac binding
proteins in Manduca sexta midgut through proteomic analysis. Insect Biochem Mol Biol 33:
999–1010
Mutz KO, Heilkenbrinker A, Lonne M, Walter JG, Stahl F (2013) Transcriptome analysis using
next-generation sequencing. Curr Opin Biotechnol 24:22–30
omez
Nagalakshmi U, Wang Z, Waern K, Shou C, Raha D, Gerstein M, Snyder M (2008)

The transcriptional landscape of the yeast genome defined by RNA sequencing. Science 320:
1344–1349
Nagamatsu Y, Toda S, Yamaguchi F, Ogo M, Kogure M, Nakamura M, Shibata Y, Katsumoto T
(1998) Identification of Bombyx mori midgut receptor for Bacillus thuringiensis insecticidal
CryIA(a) toxin. Biosci Biotechnol Biochem 62:718–726
Nagamatsu Y, Koike T, Sasaki K, Yoshimoto A, Furukawa Y (1999) The cadherin-like protein is
essential to specificity determination and cytotoxic action of the Bacillus thuringiensis insecti-
cidal CryIAa toxin. FEBS Lett 460:385–390
Nakane PK (1968) Simultaneous localization of multiple tissue antigens using the peroxidase-
labeled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem 16:
557–560
Ochoa-Campuzano C, Real MD, Martinez-Ramirez AC, Bravo A, Rausell C (2007) An ADAM
metalloprotease is a Cry3Aa Bacillus thuringiensis toxin receptor. Biochem Biophys Res
Commun 362:437–442
Ochoa-Campuzano C, Sanchez J, Garcia-Robles I, Real MD, Rausell C, Sanchez J (2012)
Identification of a calmodulin-binding site within the domain I of Bacillus thuringiensis
Cry3Aa toxin. Arch Insect Biochem Physiol 81:53–62
Oltean DI, Pullikuth AK, Lee HK, Gill SS (1999) Partial purification and characterization of
Bacillus thuringiensis Cry1A toxin receptor A from Heliothis virescens and cloning of the
corresponding cDNA. Appl Environ Microbiol 65:4760–4766
Oppert B, Dowd SE, Bouffard P, Li L, Conesa A, Lorenzen MD, Toutges M, Marshall J,
Huestis DL, Fabrick J, Oppert C, Jurat-Fuentes JL (2012) Transcriptome profiling of the
intoxication response of Tenebrio molitor larvae to Bacillus thuringiensis Cry3Aa protoxin.
PLoS One 7:e34624
Pacheco S, Gomez I, Arenas I, Saab-Rincon G, Rodriguez-Almazan C, Gill SS, Bravo A,
Soberon M (2009) Domain II loop 3 of Bacillus thuringiensis Cry1Ab toxin is involved in a
“ping pong” binding mechanism with Manduca sexta aminopeptidase-N and cadherin recep-
tors. J Biol Chem 284:32750–32757
Park Y, Kim Y (2013) RNA interference of cadherin gene expression in Spodoptera exigua reveals
its significance as a specific Bt target. J Invertebr Pathol 114:285–291
Park Y, Abdullah MA, Taylor MD, Rahman K, Adang MJ (2009) Enhancement of Bacillus
thuringiensis Cry3Aa and Cry3Bb toxicities to coleopteran larvae by a toxin-binding fragment
of an insect cadherin. Appl Environ Microbiol 75:3086–3092
Pauchet Y, Wilkinson P, Vogel H, Nelson DR, Reynolds SE, Heckel DG, ffrench-Constant RH
(2010) Pyrosequencing the Manduca sexta larval midgut transcriptome: messages for diges-
tion, detoxification and defence. Insect Mol Biol 19:61–75
Pigott CR, Ellar DJ (2007) Role of receptors in Bacillus thuringiensis crystal toxin activity.
Microbiol Mol Biol Rev 71:255–281
Quan GX, Kanda T, Tamura T (2002) Induction of the white egg 3 mutant phenotype by injection
of the double-stranded RNA of the silkworm white gene. Insect Mol Biol 11:217–222
Rabilloud T, Lelong C (2011) Two-dimensional gel electrophoresis in proteomics: a tutorial.
J Proteomics 74:1829–1841
Rajagopal R, Sivakumar S, Agrawal N, Malhotra P, Bhatnagar RK (2002) Silencing of midgut
aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as
Bacillus thuringiensis toxin receptor. J Biol Chem 277:46849–46851
Rausell C, De Decker N, Garcia-Robles I, Escriche B, Van Kerkhove E, Real MD, Martinez-
Ramirez AC (2000) Effect of Bacillus thuringiensis toxins on the midgut of the nun moth
Lymantria monacha. J Invertebr Pathol 75:288–291
Ren XL, Chen RR, Zhang Y, Ma Y, Cui JJ, Han ZJ, Mu LL, Li GQ (2013) A Spodoptera exigua
cadherin serves as a putative receptor for Bacillus thuringiensis Cry1Ca toxin and shows
differential enhancement of Cry1Ca and Cry1Ac toxicity. Appl Environ Microbiol 79:
5576–5583
Ren XL, Ma Y, Cui JJ, Li GQ (2014) RNA interference-mediated knockdown of three putative
aminopeptidases N affects susceptibility of Spodoptera exigua larvae to Bacillus thuringiensis
Cry1Ca. J Insect Physiol 67C:28–36
Rothberg JM, Hinz W, Rearick TM, Schultz J, Mileski W, Davey M, Leamon JH, Johnson K,
Milgrew MJ, Edwards M, Hoon J, Simons JF, Marran D, Myers JW, Davidson JF, Branting A,
Nobile JR, Puc BP, Light D, Clark TA, Huber M, Branciforte JT, Stoner IB, Cawley SE,
Lyons M, Fu Y, Homer N, Sedova M, Miao X, Reed B, Sabina J, Feierstein E, Schorn M,
Alanjary M, Dimalanta E, Dressman D, Kasinskas R, Sokolsky T, Fidanza JA, Namsaraev E,
McKernan KJ, Williams A, Roth GT, Bustillo J (2011) An integrated semiconductor device
enabling non-optical genome sequencing. Nature 475:348–352
Sangadala S, Walters FS, English LH, Adang MJ (1994) A mixture of Manduca sexta amino-
peptidase and phosphatase enhances Bacillus thuringiensis insecticidal CryIA(c) toxin binding
and 86Rb(+)-K+ efflux in vitro. J Biol Chem 269:10088–10092
Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression
patterns with a complementary DNA microarray. Science 270:467–470
Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W,
Granzow M, Ragg T (2006) The RIN: an RNA integrity number for assigning integrity values
to RNA measurements. BMC Mol Biol 7:3
Shields VD, Hildebrand JG (2001) Recent advances in insect olfaction, specifically regarding
the morphology and sensory physiology of antennal sensilla of the female sphinx moth
Manduca sexta. Microsc Res Tech 55:307–329
Simpson R, Burgess EPJ, Markwick NP (1997) Bacillus thuringiensis delta-endotoxin binding
sites in two Lepidoptera, Wiseana spp. and Epiphyas postvittana. J Invertebr Pathol 70:
136–142
Sivakumar S, Rajagopal R, Venkatesh GR, Srivastava A, Bhatnagar RK (2007) Knockdown of
aminopeptidase-N from Helicoverpa armigera larvae and in transfected Sf21 cells by RNA
interference reveals its functional interaction with Bacillus thuringiensis insecticidal protein
Cry1Ac. J Biol Chem 282:7312–7319
Soberon M, Gill SS, Bravo A (2009) Signaling versus punching hole: how do Bacillus
thuringiensis toxins kill insect midgut cells? Cell Mol Life Sci 66:1337–1349
Soberon M, Pardo L, Munoz-Garay C, Sanchez J, Gomez I, Porta H, Bravo A (2010)
Pore formation by Cry toxins. Adv Exp Med Biol 677:127–142
Talekar NS, Shelton AM (1993) Biology, ecology, and management of the diamondback moth.
Terenius O, Papanicolaou A, Garbutt JS, Eleftherianos I, Huvenne H, Kanginakudru S,
Albrechtsen M, An C, Aymeric JL, Barthel A, Bebas P, Bitra K, Bravo A, Chevalier F,
Collinge DP, Crava CM, de Maagd RA, Duvic B, Erlandson M, Faye I, Felfoldi G,
Fujiwara H, Futahashi R, Gandhe AS, Gatehouse HS, Gatehouse LN, Giebultowicz JM,
Gomez I, Grimmelikhuijzen CJ, Groot AT, Hauser F, Heckel DG, Hegedus DD, Hrycaj S,
Huang L, Hull JJ, Iatrou K, Iga M, Kanost MR, Kotwica J, Li C, Li J, Liu J, Lundmark M,
Matsumoto S, Meyering-Vos M, Millichap PJ, Monteiro A, Mrinal N, Niimi T, Nowara D,
Ohnishi A, Oostra V, Ozaki K, Papakonstantinou M, Popadic A, Rajam MV, Saenko S,
Simpson RM, Soberon M, Strand MR, Tomita S, Toprak U, Wang P, Wee CW, Whyard S,
Zhang W, Nagaraju J, Ffrench-Constant RH, Herrero S, Gordon K, Swevers L, Smagghe G
(2011) RNA interference in Lepidoptera: an overview of successful and unsuccessful studies
and implications for experimental design. J Insect Physiol 57:231–245
Tetreau G, Bayyareddy K, Jones CM, Stalinski R, Riaz MA, Paris M, David JP, Adang MJ,
Despres L (2012) Larval midgut modifications associated with Bti resistance in the yellow
fever mosquito using proteomic and transcriptomic approaches. BMC Genomics 13:248
Tian H, Peng H, Yao Q, Chen H, Xie Q, Tang B, Zhang W (2009) Developmental control of a
lepidopteran pest Spodoptera exigua by ingestion of bacteria expressing dsRNA of a
non-midgut gene. PLoS One 4:e6225
omez
Vachon V, Paradis MJ, Marsolais M, Schwartz JL, Laprade R (1995) Ionic permeabilities induced
by Bacillus thuringiensis in Sf9 cells. J Membr Biol 148:57–63
Vadlamudi RK, Ji TH, Bulla LA Jr (1993) A specific binding protein from Manduca sexta for the
insecticidal toxin of Bacillus thuringiensis subsp. berliner. J Biol Chem 268:12334–12340
Vadlamudi RK, Weber E, Ji I, Ji TH, Bulla LA Jr (1995) Cloning and expression of a receptor for
an insecticidal toxin of Bacillus thuringiensis. J Biol Chem 270:5490–5494
Valaitis AP, Jenkins JL, Lee MK, Dean DH, Garner KJ (2001) Isolation and partial character-
ization of gypsy moth BTR-270, an anionic brush border membrane glycoconjugate that binds
Bacillus thuringiensis Cry1A toxins with high affinity. Arch Insect Biochem Physiol 46:
186–200
Van Rie J (2000) Bacillus thuringiensis and its use in transgenic insect control technologies. Int J
Med Microbiol 290:463–469
Van Rie J, Jansens S, Hofte H, Degheele D, Van Mellaert H (1989) Specificity of Bacillus
thuringiensis delta-endotoxins. Importance of specific receptors on the brush border membrane
of the mid-gut of target insects. Eur J Biochem 186:239–247
Whyard S, Singh AD, Wong S (2009) Ingested double-stranded RNAs can act as species-specific
insecticides. Insect Biochem Mol Biol 39:824–832
Yang Y, Zhu YC, Ottea J, Husseneder C, Leonard BR, Abel C, Huang F (2010) Molecular
characterization and RNA interference of three midgut aminopeptidase N isozymes from
Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea
saccharalis. Insect Biochem Mol Biol 40:592–603
Zhuang M, Oltean DI, Gomez I, Pullikuth AK, Soberon M, Bravo A, Gill SS (2002) Heliothis
virescens and Manduca sexta lipid rafts are involved in Cry1A toxin binding to the midgut
epithelium and subsequent pore formation. J Biol Chem 277:13863–13872
Zou Z, Najar F, Wang Y, Roe B, Jiang H (2008) Pyrosequence analysis of expressed sequence tags
for Manduca sexta hemolymph proteins involved in immune responses. Insect Biochem Mol
Biol 38:677–682
Zuniga-Navarrete F, Gomez I, Pena G, Bravo A, Soberon M (2013) A Tenebrio molitor
GPI-anchored alkaline phosphatase is involved in binding of Bacillus thuringiensis Cry3Aa
to brush border membrane vesicles. Peptides 41:81–86
Chapter 5
Non-Bt Soil Microbe-Derived Insecticidal
Proteins
Leela Alamalakala, Srinivas Parimi, Sandip Dangat, and Bharat R. Char
5.1 Introduction
Although soil microbes have contributed immensely to world agriculture through

their potential biochemical attributes in the areas of insect pest management, crop
nutrient enhancement [increasing nutrient availability by plant growth-promoting
rhizobacteria (PGPR), phosphate-solubilizing bacteria (PSB)], and soil fertility
management [through metal detoxification, mycorrhizal-helping bacteria (MHB),
and arbuscular mycorrhizal fungi (AMF)] (Khan 2005), innovations in biotechno-
logy have opened new vistas for enhancing the contribution of the soil microbial
diversity to agricultural productivity. For many decades, pest control programs in
agriculture and public health have relied heavily on the use of broad-spectrum
chemical insecticides. However, the use of chemical insecticides came under
scrutiny since the early 1960s when the environmental classic Silent Spring was
published (Carson 1962), leading to a paradigm shift in insect pest management
strategies and emphasizing the need to identify non-chemical pest control strategies
that are insect-specific and environmentally safe. Naturally occurring microbial
entomopathogens, such as bacteria, fungi, viruses, and nematodes, are effective
non-chemical alternatives for the suppression and management of insect pests
causing economic losses in different crops (Lacey et al. 2001; Lacey and Kaya
2007; Shahid et al. 2012). Characterization of the genes and genomes of these
entomopathogens has facilitated the identification and deployment of novel insecti-
cidal genes for crop protection. Of all entomopathogens, bacteria have been the
most extensively used organisms to date, and overwhelming commercial success
was achieved with Bacillus thuringiensis (Bt) (Firmicutes: Bacillaceae) toxins.
Lepidoptera, the second largest insect order, comprised of moths and butterflies,
represents a diverse and important group of insect pests that affect commercial
L. Alamalakala (*) • S. Parimi • S. Dangat • B.R. Char

Maharashtra Hybrid Seeds Company Limited, Dawalwadi, PO Box 76, Jalna 431203, India

DOI 10.1007/978-3-319-14499-3_5
90 L. Alamalakala et al.
agriculture, causing widespread economic damage on food and fiber crop plants,
fruit trees, forests, and stored grains. The larval stage of the moths is detrimental to an
array of economically valuable crops including cotton, tobacco, tomato, corn,
sorghum, pulses, and wheat (Srinivasan et al. 2006). Some examples of lepidopteran
pests include: the cotton bollworms, Helicoverpa armigera (Hübner) and
Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae); the gypsy moth, Lymantria
dispar (Linnaeus) (Lepidoptera: Lymantriidae), a voracious defoliator of Palaearctic
and Nearctic forests (Reineke et al. 1999); the diamondback moth (DBM), Plutella
xylostella (Linnaeus) (Lepidoptera: Plutellidae), a pest of cole crops (Talekar and
Shelton 1993); brinjal shoot and fruit borer, Leucinodes orbonalis (Guenée) (Lepi-
doptera: Crambidae); and okra shoot and fruit borer, Earias vittella (Fabricius)
(Lepidoptera: Noctuidae) which causes 69 % loss in marketable yield in okra
(Radake and Undirwade 1981). Until recently, the control of pests in agriculture
has mostly relied on the intensive application of broad-spectrum synthetic insecti-
cides, with about 40 % targeted to the control of lepidopteran insects (Brooke and
Hines 1999). Over the years the application of insecticides has led to the develop-
ment of insecticide-resistant insects, destruction of natural enemies as well as
harmful effects on humans and the environment. Therefore an urgent need was felt
for alternative control strategies that reduce dependence on conventional insecti-
cides. The interest in biopesticides started growing significantly, as a result of the
withdrawals of many synthetic pesticides and the high cost of developing new ones.
In this scenario, it is important to note that the global pesticide market is growing at a
compound annual growth rate (CAGR) of 3.6 % and the value is expected to reach
$51 billion in 2014. The biopesticide segment which represents a strong growth
arena in the global pesticide market is expected to grow at a 15.6 % CAGR from $1.6
billion to $3.3 billion in 2014 (BCC Research 2010; Ruiu et al. 2013). Thus, the
direct application of entomopathogens as biological control agents or deploying GM
crops developed using novel entomotoxic proteins provides a good market oppor-
tunity that can be captured by the industry. Agriculture was perceived to benefit from
futuristic eco-friendly strategies such as the use of natural enemies, autocidal control
methods such as sterile insect technique (SIT) and F1 sterility, and transgenic plants
expressing entomotoxic proteins (Fitt 1994; Gatehouse et al. 1994; Haq et al. 2004;
Saour 2014).
B. thuringiensis (Bt) is a gram-positive bacterium that is found in a variety of
ecological niches such as soil, water, plant surfaces, stored cereals and dead insects
(Federici and Siegel 2008). The bacteria form spores containing proteinaceous
crystals known as Cry or Cyt proteins (also known as δ-endotoxins), as well as
VIPs (vegetative insecticidal proteins) exhibiting potent insecticidal activity
(Sanahuja et al. 2011). Different strains of Bt produce different types of insect-
toxic virulence factors, and the activity of these virulence factors toward Lepidop-
tera, Diptera, Coleoptera, Hymenoptera, Homoptera, Orthoptera, and Mallophaga
insect orders have been reported (Schnepf et al. 1998). Bt was released as a
biopesticide (ICP and viable spores) since 1951 (Steinhaus 1951) and formulations
based on Bt (67 registered products and more than 450 formulations) occupied the
key position accounting for nearly 90 % of the total biopesticide sales worldwide
(Neale 1997). Bt products used for managing lepidopteran pests were primarily
5 Non-Bt Soil Microbe-Derived Insecticidal Proteins 91
derived from Bt Kurstaki HD-1 strain (e.g., Biobit, Dipel, and Thuricide) and to a
lesser extent from Kurstaki SA-11 and Kurstaki SA-12 strains (Kaur 2000). How-
ever, Bt had limited use as a foliar insecticide due to the short window of effec-
tiveness as a result of which multiple sprays had to be undertaken which led to
increase in the amount of product for application and fuel needed for spraying. The
sprays also had little impact on cryptic pests (Sanchis 2011). These inherent
limitations of topical Bt pesticides were overcome by introducing Bt cry genes
into target crops thereby enhancing plant health and conferring plant protection
(Sanahuja et al. 2011).
Transgenic crops protected from insect pests have become an integral part of
insect pest management (IPM) with over 58 Mha planted worldwide in 2010 (James
2010; Baum et al. 2012). A number of transgenic crops including corn, cotton, rice
and soybean harboring Bt genes are cultivated commercially since 1996 (Huang
et al. 2007; Sanahuja et al. 2011). Bt cotton, in particular, has provided effective
control of several lepidopteran pest species including tobacco budworm, Heliothis
virescens (Fabricius) (Lepidoptera: Noctuidae), pink bollworm, Pectinophora
gossypiella (Saunders) (Lepidoptera: Gelechiidae), and the cotton bollworm,
H. armigera (Hübner) (Lepidoptera: Noctuidae), resulting in increased yield,
reduced frequency of insecticide applications, and area-wide suppression of the
same primary insect pest in other crops (Perlak et al. 2001; Carrière et al. 2003;
Jackson et al. 2003; Wu et al. 2008; James 2010). The widespread cultivation of Bt
cotton varieties can contribute to a resurgence in beneficial arthropod populations
necessary for successful IPM (Head et al. 2005; Naranjo 2005; Whitehouse
et al. 2005). Although Bt-derived biopesticides used as foliar sprays or expressed
in plants through genetic engineering are environmentally safe and effective, their
use is still restricted due to problems of limited host range and the potential for the
development of resistance and cross resistance in key pests due to continuous use,
thus necessitating the discovery of novel insecticidal genes with improved activity
and host range. Field evolved resistance to Bt crops has been reported for
populations of several insect pests (Gassmann et al. 2011).
Insecticidal nematodes in the genera Steinernema and Heterorhabditis, each
carrying a specific genus of bacteria, are the only insect-parasitic nematodes
possessing an optimal balance of biological control attributes (Poinar 1979; Bed-
ding et al. 1993), and they have been used for the biological control of soil-dwelling
pests that include weevils and lepidopteran insects (Wang and Li 1987; Klein
1990). However, factors such as cost, shelf life, handling, mixing, compatibility,
and profit margins to manufacturers and distributors have contributed to the failure
of entomopathogenic nematodes (EPN) especially for large-scale agriculture appli-
cations, as they failed to penetrate many markets or gain significant market share in
the current markets (Lacey and Georgis 2012). The widespread adoption and
success of Bt crops and the associated risk of resistance development to Bt
protein(s) have stimulated the research and development of more environmentally
responsible alternatives. Thus, a significant research need was centered toward the
characterization of insecticidal proteins, called the toxin complex (Tc) proteins and
other virulence factors from bacteria that are symbionts of EPNs as they can be used
for the development of a new generation of GM crops that are protected against a
wider spectrum of insect pests (Bowen and Ensign 1998).
Sequencing, annotation, and screening of the genomes of entomopathogenic
bacteria such as Photorhabdus, Xenorhabdus, and Pseudomonas spp. have begun
to reveal previously unidentified insecticidal toxins. The presence of orthologues of
a multitude of insecticidal genes (tc, mcf1 and mcf2, xaxAB) in different species of
insect-pathogenic bacteria indicates that a large amount of genetic transfer occurs
between these species, presumably within a shared environmental niche (Hinchliffe
et al. 2010), thus providing an excellent source of novel candidates, which can be
used as potential alternatives to the insecticidal proteins derived from
B. thuringiensis. The toxin complex proteins from Photorhabdus luminescens
have also been transferred into plants and tested for their activity on different insect
pests. The intent of this book chapter is to provide a comprehensive review of
virulence factors produced by gram-negative entomopathogenic bacteria that
demonstrate potential toxicity toward lepidopteran pests so that they could be
successfully exploited for plant protection.
5.2 A Vast Arsenal of Insecticidal Toxins Derived from

Gram-Negative Bacteria
Although deployment of crops expressing insecticidal proteins has led to effective

control of insect pests and reduced the use of chemicals for insect control, very few
candidate proteins have been commercially used for crop protection. These proteins
typically control limited ranges of pest species and are predominantly from gram-
positive bacteria and derived mostly from B. thuringiensis. Entomopathogenic
gram-negative bacteria also produce toxins that are harmful to insects and can be
used to augment the list of genes used in developing pest control products. Mem-
bers in the Enterobacteriaceae such as Photorhabdus, Xenorhabdus, Serratia,
Pseudomonas, and Yersinia spp. produce insecticidal toxins with toxicity similar
to that of Bt toxins. Initial studies showed that these bacteria were highly effective
when fed to a range of pest species belonging to at least three orders of
insects including the Lepidoptera. Based on their targeted tissue, these toxins
can be categorized into three types: (a) cytotoxins, (b) digestive toxins, and
(c) neurotoxins (Castagnola and Stock 2014). This section describes the virulence
factors associated with different gram-negative bacteria, their activity spectrum,
and mode of action.
5.2.1 Insect Virulence Factors Produced by Photorhabdus

Species
The Photorhabdus genus currently consists of three species: P. luminescens,

Photorhabdus temperata, and Photorhabdus asymbiotica, found in a symbiotic
association with an insect-pathogenic soil nematode of the genus Heterorhabditis.
Several subspecies are recognized (Fischer-Le Saux et al. 1999). The genus
Photorhabdus and the two species P. luminescens and P. temperata have been
the subject of intensive study by entomologists and agricultural scientists in view of
their insect pathogenicity and their potential for the development of novel
biopesticides and insect-resistant transgenic plants. P. asymbiotica is a human
pathogen that has been recovered from human clinical specimens from the USA
and Australia and is currently considered an emerging human pathogen model
system (Gerrard et al. 2004; Gerrard et al. 2006).
Photorhabdus are gram-negative, bioluminescent, motile bacteria of the family
Enterobacteriaceae which live in an obligate mutualistic association with insect-
parasitic Heterorhabditis nematodes, which invade and kill insects in the soil
(Waterfield et al. 2009). The infective juvenile (IJ) nematode exists as a free-living,
non-feeding individual in the soil and actively seeks out and colonizes the insect
prey in the soil. The IJ nematode enters a potential victim either through respiratory
spiracles, the mouth, or the anus. The symbiotic bacteria vectored by these IJs are
then regurgitated from the nematode intestine into the open circulatory system of
the insect prey. The bacteria colonize the anterior midgut of the insect initially,
undergo rapid multiplication, and subsequently kill the insect within 1–2 days. As
the bacterial population reaches a high level, the insect cadaver becomes red in
color and visibly bioluminescent (Bowen and Ensign 1998). During the growth in
the insect prey, the bacteria release a plethora of virulence factors to kill the insect
and produce antibiotics with antifungal and antibacterial activities that probably
prevent the invasion of the cadaver by other microorganisms resulting in ideal
conditions for the growth and reproduction of the nematode (Paul et al. 1981;
Akhurst 1982).
P. luminescens appears to encode numerous putative (ffrench-Constant
et al. 2000) and proven (Bowen et al. 1998; Waterfield et al. 2001) insect virulence
factors in its genome. The Photorhabdus genome is organized into genomic islands
relating both to pathogenicity and to symbiosis. Genomic islands involved in
pathogenicity are called “pathogenicity islands” (PAIs). PAIs are unstable regions
that are present in the pathogen but absent from non-pathogens (Hacker and Kaper
2000) and are often inserted next to tRNA genes and have differing GC content
from the rest of the genome (Waterfield et al. 2002; ffrench-Constant et al. 2003).
Functional analysis of genomic islands facilitated the identification of a diversity of
anti-invertebrate virulence factors from P. luminescens (Daborn et al. 2002;
Waterfield et al. 2002). Multiple copies of “toxin complex” (tc) genes (Bowen
et al. 1998; Waterfield et al. 2001) inserted at an AspV tRNA were detected in the
first unique island. The tc genes encode high molecular weight, multi-subunit,
orally active insecticidal toxins first characterized in insect pathogens

Photorhabdus and Xenorhabdus spp. (Bowen et al. 1998; Waterfield et al. 2001),
but now seen in a range of pathogens, including those of humans. Some Tc’s have
demonstrated oral toxicity to insects making them potential candidates for insect
pest control. A second island inserted at a Phe tRNA was found to encode the novel
toxin “makes caterpillars floppy” or Mcf, a large toxin with little similarity to
known proteins (Daborn et al. 2002). A third island with a skewed GC content
contains a gene encoding a cytotoxic necrotizing factor (CNF)-like toxin, desig-
nated Pnf. The specific role of Pnf in Photorhabdus is unknown (Buetow
et al. 2001; Waterfield et al. 2002). Two copies of a macrophage-toxin-like
encoding gene similar to that found in pathogenic strains of Escherichia coli are
carried by a fourth island that also contains an rhs element and a CP4-like integrase
gene. This island is linked to the phlAB hemolysin locus and, probably forms a part
of larger region involved in pathogenicity. Lastly, a fifth island encodes a type III
secretion system (TTSS), and the order of genes in the TTSS island is similar to that
in Yersinia pestis, and these genes are probably important in the interaction of
Photorhabdus with its invertebrate hosts (ffrench-Constant et al. 2000; Waterfield
et al. 2002; Silva et al. 2002).
5.2.1.1 The Toxin Complexes (Tc’s) of Photorhabdus
The Tc proteins produced by P. luminescens are an important class of secreted

toxins, with an estimated molecular weight of 1,000,000 and with no detectable
protease, phospholipase, or hemolytic activity but showing a trace lipase activity.
The Tc is a large, multimeric complex comprising of several protein subunits
ranging in size from 30 to 200 kDa, some of which are found to be lethal when
fed to or injected into the hemolymph of Manduca sexta larvae and several other
insect species (Bowen and Ensign 1998). Purification of the active protein complex
revealed the presence of four distinct protein “toxin complexes” which were termed
Tca, Tcb, Tcc, and Tcd, and the genes corresponding to these Tc proteins were
cloned from strain W14 (Bowen et al. 1998). The different Tc’s are encoded at
discrete PAIs in the Photorhabdus genome where multiple tc gene copies are found
(Wilkinson et al. 2009). Although all Tc proteins show injectable toxicity to
M. sexta, majority of the oral toxicity of tc genes toward lepidopteran insect pests
was found to be mediated by tca and tcd genes as shown via gene knockout studies
(Bowen et al. 1998). Sequence analysis of the tca, tcb, tcc and tcd loci revealed a
high degree of similarity between loci, and despite the apparent complexity of the
loci, the individual genes within these loci could be grouped into three basic types
of genetic elements: the tcdA-like or [A], the tcaC-like or [B], and the tccC-like or
[C] (ffrench-Constant and Waterfield 2005). These groupings suggest similar roles
of their encoded proteins within the assembled toxin complex, and a representative
of [A], [B], and [C] was required for full toxicity (Waterfield et al. 2005a). The tca
locus of P. luminescens W14 consists of three open reading frames (ORFs), tcaA,
tcaB, and tcaC with the [A] subunit of the Tc encoded by tcaA and tcaB, the
[B] subunit encoded by tcaC, and the [C] subunit encoded by tccC gene from the tcc
locus. A fourth ORF the tcaZ associated with the tca locus is encoded in the
opposite orientation, and the function of this protein is not yet known. The tcb
locus consists of a single [A] gene, tcbA and the tcc locus consists of an [A] encoded
by tccA and tccB, and a [C] encoded by tccC. The tcd locus is the largest of the four
tc loci and consists of four [A] genes, tcdA1-A4; two [B] genes, tcdB1–tcdB2; and
four [C] genes, tccC2–tccC5 (Hinchliffe et al. 2010). Orthologues of tc encoding
genes are widespread in gram-negative bacteria (Xenorhabdus, Serratia, and
Yersinia) and also present in some gram-positive bacteria (Paenibacillus). The
association of these loci with transposase-like or bacteriophage-like genes, indi-
cates that they are highly mobile and can be transferred between species (Hinchliffe
et al. 2010).
The role of proteins encoded by the tc loci in Photorhabdus biology is ambi-
guous despite efforts undertaken to understand the structure, function, genetics, and
mode of action of these proteins. The Tc’s of Photorhabdus spp. appear to be very
undiscriminating in their activity, with demonstrable toxicity toward a wide spec-
trum of insect species (Hinchliffe et al. 2010); therefore, their use as candidates for
crop protection may be limited unless their effects on non-target organisms (NTO),
especially the beneficial arthropods are investigated. The insecticidal tc’s have been
shown to be preferentially expressed at low temperatures (<15 C). Analysis of the
expression and insecticidal activity of the protein subunits of the P. luminescens
W14 tcd locus revealed that the [A] subunit itself possessed a low level of toxicity
which is potentiated by [BC], a complex formed by [B] and [C] when expressed
together. The [BC] complex demonstrated mild oral toxicity toward M. sexta,
whilst the [B] and [C] subunits individually were not orally toxic. The [C] subunit
appeared to play an important role in the complex as its presence was observed to be
necessary for oral toxicity of Tca and Tcd. A thorough understanding of specific
protein interactions is therefore very crucial for these proteins to be successfully
used for insect pest control.
The mechanism of action of the Tc’s is not well understood. Although the
toxicity of the Tc proteins has been demonstrated on specific model insects and
cultured cells, comprehensive information on how these effects are mediated by the
proteins is currently unavailable. The ingestion of purified P. luminescens Tca by
M. sexta led to the complete destruction of the midgut epithelium leading to
cessation of feeding and eventual starvation of the insect host. Tca also showed
characteristic, midgut-specific histopathology in M. sexta that included the apical
swelling of the columnar cells in the epithelium of the anterior midgut and blebbing
of the vesicles into the gut lumen (Blackburn et al. 1998). No pathological effects
were observed on any other tissues indicating the gut specificity of the toxin. Liu
et al. (2006) reported that a P. luminescens Tca-like toxin (PL toxin) caused channel
formation in the midguts and permeabilized unilamellar lipid vesicles of M. sexta in
a pH-dependent manner. However, structural studies of XptA1 indicated that the
protein binds to the brush border membrane vesicles but does not form pores in the
membrane. It was therefore hypothesized that the [BC] probably aids in the
insertion of the [A] tetramer into the membrane (Hinchliffe et al. 2010). Toxin A
protein (283 kDa) was expressed in Arabidopsis thaliana using a synthetic plant-
codon-optimized variant of tcdA, and the insecticidal efficacy of the protein was
tested for control of feeding insects (Liu et al. 2003). Transgenic plants expressing
more than 700 ng/mg of extractable protein were found to be highly toxic to
M. sexta, and the toxin A purified from transgenic plants had a strong inhibitory
effect on the growth of southern corn rootworm. In the best transgenic Arabidopsis
line, high toxin A expression and insect resistance were found to be consistent for at
least five generations in all progeny (Liu et al. 2003). These results indicate that the
tc proteins from Photorhabdus may open a new route to transgenic pest control.
5.2.1.2 Photorhabdus Insect-Related Binary Toxins, PirA/B
Two genetic loci (plu4093–plu4092 and plu4437–plu4436) sharing significant

sequence similarity with a putative juvenile hormone esterase (JHEs) of
Leptinotarsa decemlineata (Vermunt et al. 1997; Duchaud et al. 2003) were
identified from the genome sequence of Pl TT01. JHEs regulate metamorphosis
by inactivating the juvenile hormones involved in maintaining the insect in a larval
state. The inappropriate activation of the insect endocrine machinery by JHE-like
proteins may therefore be an effective strategy for insect control (Bonning and
Hammock 1996). The plu4093–plu4092 and plu4437–plu4436 genetic loci were
renamed as “Photorhabdus insect-related” (Pir) proteins, with PirA referring to
products of plu4093/4437 homologs and PirB to products of plu4092/4436 homo-
logs (Waterfield et al. 2005b). Pir proteins are binary toxins having both injectable
(Waterfield et al. 2005b) and oral toxicity (Blackburn et al. 2006) toward insects
from the orders Diptera and Lepidoptera. Waterfield et al. (2005b) demonstrated
that each of the genes in the P. luminescens loci was required for toxicity when
injected into larvae of Galleria mellonella L. (Lepidoptera: Pyralidae), but the
combination was not sufficient to cause mortality in M. sexta L. (Lepidoptera:
Sphingidae) by either injection or oral administration. The oral activity of Pir A/B
tested against the diamondback moth, P. xylostella, demonstrated that the midgut is
the primary site of action. The pathology observed was similar to those seen with
other gut-active toxins, but consistent effects were noticed in the posterior midgut.
P. xylostella was found to be 300-fold more susceptible to Pir toxins than other
insect species tested (Blackburn et al. 2006). However, these proteins had no effect
on the growth or mortality of H. virescens F. (Lepidoptera: Noctuidae), M. sexta
L. (Lepidoptera: Sphingidae), or L. dispar L. (Lepidoptera: Lymantriidae) larvae on
oral delivery (Blackburn et al. 2006). Based on insect bioassays, it can be concluded
that the Pir A/B proteins may not be broadly useful as insecticidal proteins.
Although the PirB protein shares some sequence similarities with the
δ-endotoxins from B. thuringiensis, no significant difference was observed in the
responses of the susceptible P. xylostella larvae (lab colony) and the commercially
available Cry 1A-resistant strain to the PirB protein. The PirA/B proteins have been
shown to lack the esterase activity and evidence presented by Crosland et al. (2005)
suggested that these proteins are related to leptinotarsin, a neurotoxic protein
present in the hemolymph of several Leptinotarsa species (Hsiao and Fraenkel
1969). Consequently the mechanism for the potential insecticidal activity appears
to be the destructive effects on the neural tissue upon injection (Castagnola and
Stock 2014).
5.2.1.3 The Makes Caterpillars Floppy (Mcf1 and Mcf2) Toxins
The Mcf toxin is a high molecular weight protein (324 kDa) that was found to
facilitate the persistence of E. coli expressing this gene within the insect host and
kill the insects (Daborn et al. 2002). The predicted amino acid sequence of the 8.8-
Kb mcf1 gene fragment cloned from P. luminescens subsp. akhurstii strain W14
showed only partial homology to known proteins; however, it carried a BH3
domain, a domain found in pro-apoptotic proteins (Budd 2001). The Mcf toxins
are potent toxins that are active upon injection and induce apoptosis via the
mitochondrial pathway in insect phagocytes, helping the bacteria avoid phago-
cytosis. These proteins destroy the columnar and goblet cells of the insect midgut
epithelium, causing the caterpillar to lose body turgor due to impaired osmo-
regulation, and become “floppy” (Daborn et al. 2002). This toxin also promotes
apoptosis in mammalian tissue culture cells (Dowling et al. 2004). A second Mcf1-
like ORF (mcf2) which also caused loss of body turgor when injected into M. sexta
larvae was identified during end sequencing of Pl W14 cosmid library (Waterfield
et al. 2003). The two mcf proteins are 77.5 % identical across the majority of their
lengths only differing in their N-terminal regions (Hinchliffe et al. 2010). While
Mcf1 contained a long 900 amino acid N-terminal region with no similarity to other
proteins in the database, a shorter 300 amino acid N-terminal region (a HopA1-like
region) containing a domain showing similarity to several type III secreted proteins
was found in Mcf2. The region of similarity between Mcf1 and Mcf2 contains a
BH3-like domain, two domains found in RTX-like toxins, and a large domain found
in the Clostridium difficile binary toxins (Hinchliffe et al. 2010). Comparisons of
available sequence data from different Photorhabdus strains revealed that copies
of mcf1 are always present, suggesting that it may be the dominant insect-killing
toxin. However, it is very likely that mcf2 is only present in a subset of strains.
Toxins like Mcf which act on both the gut and insect immune system represent a
promising, yet underexploited avenue for future insecticide development (Daborn
et al. 2002).
5.2.1.4 Txp40 Toxin
A novel 42 kDa secreted protein encoded by the toxin gene txp40 (txp40V16,
identified from P. luminescens strain V16) and initially identified in Xenorhabdus
nematophila (A24tox, txp40A24) was found to be part of a genomic island involved
in pathogenicity and highly conserved and widespread among the Photorhabdus
strains (Brown et al. 2006). Txp40 protein was found to have hemolymph toxicity
and was effective against a range of lepidopteran species (G. mellonella,
H. armigera, and Plodia interpunctella) and the dipteran species Lucilia cuprina.
The protein exhibited significant cytotoxicity in vitro against two dipteran cell lines
(Aedes aegypti and Drosophila melanogaster cell line S2) and two lepidopteran cell
lines (Spodoptera cell lines Sf9 and Sf21), but not against a mammalian cell line.
The broad insecticidal activity of the Txp40 toxin suggests that the toxin has a
target that is common to many different insects (Brown et al. 2006). Gut histology
studies of H. armigera showed that the midgut and fat body are the targets and the
toxin caused a significant decrease in midgut intercellular adhesion, degradation of
the peritrophic matrix lining of the midgut cells, and degradation of the fat body
nuclei. Although the selective toxicity of Txp40 against a broad spectrum of
lepidopteran insect pests, and the lack of toxicity against mammalian cell lines
makes it a good candidate for pest control, the protein has to retain its insect-toxic
properties (it should not be degraded by the insect gut enzymatic machinery) upon
oral delivery for it to be a potential option for developing transgenic crops.
5.2.1.5 An Array of Other Insecticidal Proteins from Photorhabdus
Photorhabdus Insecticidal Toxin
Photorhabdus insecticidal toxin (Pit), a probable toxin from P. luminescens show-

ing 30 % amino acid sequence similarity to a fragment of a 13.6 kDa insecticidal
crystal protein gene of B. thuringiensis, demonstrated injectable toxicity to the
larvae of G. mellonella and Spodoptera litura. However, ingestion of purified Pit
protein caused an inhibition of growth of S. litura and H. armigera larvae, but did
not cause larval mortality. The hemocoel insecticidal activity of Pit was comparable
with other hemocoel toxins such as Txp40 of Photorhabdus (Li et al. 2009).
Photorhabdus Virulence Cassettes
Photorhabdus virulence cassettes (PVCs) are phage-like loci found as repetitive

cassettes in the genome of Photorhabdus and contain putative toxin effector genes.
PVCs are functional homologues of the prophage-like locus on the pADAP plasmid
of Serratia entomophila (Yang et al. 2006). Recombinant expression of various
PVC loci from P. luminescens and P. asymbiotica demonstrated that they have
differing toxicities toward G. mellonella upon injection with PVC product derived
from the human pathogen P. asymbiotica (Gerrard et al. 2004) having greater
toxicity for insects than PVC product from the insect pathogen P. luminescens
TT01 (Duchaud et al. 2003). Although the PVC products showed structural simi-
larity to an antibacterial R-type pyocin, they had no conspicuous antibacterial
activity but triggered rapid destruction of insect phagocytes, thus allowing the
persistence of recombinant bacteria in wax moth, G. mellonella larvae. Comparison
of the genomic organizations of PVCs in different Photorhabdus species revealed
that they have a conserved phage-like structure with a variable number of putative
anti-insect effectors encoded at one end. Expression of these putative effectors
directly inside cultured cells showed that they are capable of rearranging the actin
cytoskeleton (Yang et al. 2006).
Hemolysins or Hemagglutinin-Related Proteins
Hemolysins are extracellular toxic proteins that function as virulence factors and
derive their name because of their activity toward red blood cells (Brillard
et al. 2002; Cowles and Goodrich-Blair 2005). Hemolysins are produced by a
wide spectrum of bacterial species that include the gram-positive (e.g., Listeria
spp., Streptococcus spp.) and the gram-negative (e.g., E. coli, Vibrio spp.,
Photorhabdus spp., Xenorhabdus spp., Serratia spp.) bacteria, and these proteins
frequently target the immune cells and may aid in evading insect immune responses
during infection (Konig et al. 1987; Swihart and Welch 1990; Cowles and
Goodrich-Blair 2005). P. luminescens phlBA operon, a locus encoding a hemolysin,
shows similarities to the pore-forming, calcium-independent hemolysins from
S. marcescens, and Proteus mirabilis type of hemolysins, and belongs to the
two-partner secretion (TPS) family of proteins (Brillard et al. 2002). Hemolysins
target red blood cells to provide access to iron and may mediate the successful
occupation of the different host environments (nematode and insect) it encounters
during its life cycle. In case of X. nematophila, XhlA (X. nematophila haemolysin)
was observed to be necessary for full virulence against M. sexta larvae (Cowles and
Goodrich-Blair 2005).
5.2.2 Insect Virulence Factors Produced by Xenorhabdus

Species
Xenorhabdus species are motile gram-negative bacteria of the family Entero-

bacteriaceae that are mutualistic symbionts of the soil-dwelling nematodes from the
family Steinernematidae. The life cycle of Xenorhabdus is similar to that described
for Photorhabdus, with the Steinernema nematodes playing a key role in vectoring
these bacteria from one host to another (Hinchliffe et al. 2010; Castagnola and Stock
2014). Although both types of bacteria are mutualists with nematodes and are
entomopathogens, they use distinct, functionally different approaches for these
roles (Poinar 1993; Griffin et al. 2001; Goodrich-Blair and Clarke 2007), suggesting
that Xenorhabdus and Photorhabdus underwent divergent evolution that arrived at
convergent lifestyles (Chaston et al. 2011). The ingestion of hemolymph was found to
trigger the release of Xenorhabdus through the anus of nematode host,
S. carpocapsae. Five species (Xenorhabdus beddingii, Xenorhabdus bovienii,
Xenorhabdus japonicus, Xenorhabdus nematophilus, and Xenorhabdus poinarii)
were recognized in the genus Xenorhabdus after initial reclassification, and a total
of 15 new species have been identified from Steinernema nematode collections
since then (Lengyel et al. 2005; Somvanshi et al. 2006; Tailliez et al. 2006).
Of these, the best studied nematode-bacterial associations are those of X. nematophila–

S. carpocapsae, and it has been demonstrated that certain Steinernema–-
Xenorhabdus associations are exclusive and non-cognate pairs will not associate
during experimental mixing (Akhurst 1983; Sicard et al. 2004). The complete
genomes of X. nematophila ATCC 19061 and X. bovienii SS-2004 have been
sequenced (Chaston et al. 2011).
Xenorhabdus overcomes the insect’s defense systems and produces an array of
virulence factors (proteases, lipases, hemolysins, immunosuppressants, and toxins)
that participate in suppressing insect immunity and killing the host (Forst and
Nealson 1996). Two types of hemocytes (the granulocytes and plasmatocytes)
comprise greater than 70 % of the cells found in the lepidopteran larval hemolymph
(Gillespie et al. 1997). During X. nematophila infection, the overall numbers of
circulating insect hemocytes are drastically reduced (da Silva et al. 2000). Two
factors (C1 and C2) that are produced in liquid cultures and target the insect
hemocytes have been identified in Xenorhabdus (Brillard et al. 2001). Two cyto-
toxins, the αX and Xax, having identical biological effects on insect hemocytes and
associated with C1 factor have been characterized from Xenorhabdus (Ribeiro
et al. 2003; Vigneux et al. 2007). The toxin complex genes (xpt) encoding high
molecular weight insecticidal proteins have also been observed in X. nematophila.
Xenorhabdus xpt genes exist on a pathogenicity island (PAI) like the tc genes of
Photorhabdus, and it has been demonstrated that the PAIs of strains of
Xenorhabdus are nearly identical, indicating that the presence of PAIs corresponds
to either an evolutionary advantage or increased fitness (Sergeant et al. 2006).
5.2.2.1 The Toxin Complexes (Tc’s) of Xenorhabdus
X. nematophila contains only a single tc locus encoding all three subunits with two
[A] genes, xptA1 and xptA2; a single [B] gene, xptC; and a single [C] gene, xptB.
These show the greatest levels of identity to the P. luminescens genes tcdA, tcaC,
and tccC, respectively (Hinchliffe et al. 2010). The native toxin complex (toxin
complex 1) from Xenorhabdus is composed of three different proteins XptA2 [284
kDa], XptB1 [110 kDa], and XptC1 [158 kDa], representing class A, B, and C
proteins that were found to interact in a 4:1:1 (XptA2:XptB1:XptC1) stoichiometry
(Sheets et al. 2011), while Xenorhabdus tc2 contains XptA1 [287 kDa], in addition
to XptB1 and XptC1 where the two separate [A} genes, XptA1 and XptA2, have
been shown to be responsible for different host species specificity within the tc’s
(Lee et al. 2007). XptA1 protein confers specificity toward Pieris brassicae and
Pieris rapae, and the XptA2 protein confers specificity toward H. virescens (Ser-
geant et al. 2003). This indicates that these [A] subunits must interact with some
kind of specific receptor in order for the complex to cause toxicity. The tc gene
products can be categorized into toxins and potentiators. The potentiators synergize
with their Tc toxin counterpart for full insecticidal activity (Waterfield et al. 2005a).
The A component of Tc complexes has toxin activity potentiated by the
[BC] components, and this has been demonstrated for the Tc’s of X. nematophila
as well (Sergeant et al. 2003). All three components were found essential for the
formation of a biologically active toxin complex. Although the Tc’s have often
been thought of as being possible pore-forming toxins, Lee et al. (2007) observed
that purified XptA1 binds specifically to brush border membrane vesicles (BBMV)
from P. brassicae and to Sf21 cells but does not form pores in the membranes. It is
likely that the [A] tetramer alone cannot form pores, whilst a mature complex
containing [BC] subunits can, thereby suggesting that [BC] is probably mediating
the insertion of [A] into membranes and thus “potentiating” the toxicity of
[A] (Hinchliffe et al. 2010).
5.2.2.2 Txp40 Toxin
Brown et al. (2004) described a novel 42 kDa toxin, A24tox, from X. nematophila
strain A24 that had a lethal with effect on lepidopteran larvae such as G. mellonella
and H. armigera when injected at doses of 30 to 40 ng/g larvae. Injection of the
A24tox protein into lepidopteran larvae caused the larvae to cease feeding almost
immediately, indicating that the midgut may be the primary site of action for the
toxin. Detection, characterization and alignment of the txp40 gene sequences from
several strains of Xenorhabdus and Photorhabdus highlighted the conserved nature
of the gene and its ubiquitous occurrence within this group. (Brown et al. 2006;
Castagnola and Stock 2014). The insecticidal activity of the Txp40 toxin and the
histopathology of larvae treated with the toxin are similar to those observed with the
Photorhabdus Txp40 protein and discussed in detail in the Sect. 5.2.1.4.
5.2.2.3 Insecticidal Pilin Protein
All gram-negative bacterial pathogens have been shown to secrete their virulence
factors enclosed in outer membrane vesicles (OMVs) (Beveridge 1999). The
naturally secreted OMVs of X. nematophilus contained a number of proteins and
showed larvicidal activity when they were incorporated into the diet of neonatal
larvae of H. armigera (Khandelwal and Banerjee-Bhatnagar 2003). A 17 kDa pilin
subunit protein present in the X. nematophila OMV was found to be cytotoxic to the
cultured larval hemocytes of H. armigera, causing agglutination, and subsequent
release of the cytoplasmic enzyme lactate dehydrogenase (Khandelwal et al. 2004).
The 17 kDa pilin subunit demonstrated oral toxicity to the fourth or fifth instar
larvae of H. armigera in a dose-dependent manner, causing the breakdown of the
gut epithelial lining, thereby affecting the integrity of the cellular lining, resulting in
the sloughing of the cell debris into the lumen (Khandelwal et al. 2004).
5.2.2.4 Insecticidal GroEL Protein
XnGroEL is a ~58 kDa OMV protein secreted by X. nematophilus that belongs to a

highly conserved family of molecular chaperones and is required for the proper
folding of cellular proteins. XnGroEL has chitin-binding property and interacts
with the larval peritrophic lining, and oral ingestion of this protein caused inhibition
of the growth and development of H. armigera larvae. While all three domains
(apical, intermediate, and equatorial) of the protein were found to be necessary for
optimal insecticidal activity, two surface-exposed residues Thr-347 and Ser-356 in
the apical domain were found to be vitally important for binding to the gut
epithelium and insect-toxicity (Joshi et al. 2008). The oral toxicity of XnGroEL
against H. armigera, evaluated by transgenic expression of the protein in tobacco,
showed 100 % reduction in the larval survival on transgenic plants (Kumari
et al. 2014).
5.2.2.5 Xenorhabdus Alpha-Xenorhabdolysin (Xax) Toxin
The XaxAB cytotoxin produced by X. nematophila is encoded by two genes xaxA

and xaxB and appears to be the prototype of a new family of binary toxins based on
molecular characterization of the locus, gene and amino acid sequences. Xax
triggers apoptosis in both insect (Spodoptera littoralis) hemocytes and mammalian
cells (Vigneux et al. 2007). Active protein was produced when the two genes were
expressed in recombinant E. coli. However optimum hemolytic activity was
observed when these proteins were added to cells in vitro in a specific order
(Xax A and then Xax B) and at equal concentrations. xax genes A and B were
found to be present in the genome sequences from various bacterial pathogens of
insects (Xenorhabdus, Photorhabdus, Pseudomonas entomophila), plants (Pseudo-
monas syringae), and humans (Yersinia enterocolitica and P. mirabilis) (Vigneux
et al. 2007).
5.2.3 Insect Virulence Factors Produced by Pseudomonas

Species
Pseudomonas spp. (Enterobacteriaceae) are metabolically versatile gram-negative

bacteria that are ubiquitous in their distribution and have been recovered from a
wide variety of ecological niches, including soil and plants (Vodovar et al. 2006).
The root-associated bacteria of the genus Pseudomonas exhibiting inhibitory acti-
vity toward fungal plant pathogens have been extensively used for crop protection.
The insecticidal properties of P. entomophila, P. syringae, and Pseudomonas
fluorescens were discovered thereafter (Castagnola and Stock 2014).
P. entomophila is highly pathogenic to Drosophila melanogaster and also

exhibits considerable insecticidal potency against other insects (e.g., Bombyx
mori, Anopheles gambiae) upon ingestion. The precise mechanism of death remains
unclear, but the invading bacteria were found to be resistant to the immune response
triggered in the insect after oral ingestion (Vodovar et al. 2005). It is speculated that
P. entomophila evades the insect immune response by making proteases (alkaline
protease AprA) and exotoxins (hemolysins and lipases) that punch holes in the cell
membranes of the insect phagocytes. P. entomophila genome encodes several
gut-specific toxin complexes (Tc’s) with multiple copies of C-like elements and
one copy of a B-like gene that are not organized as a single operon but are scattered
across the genome. The genome lacks a gene encoding an A toxin which determines
the host range, suggesting that the insecticidal activities of toxin complexes from
P. entomophila may be fairly restricted. P. entomophila genome encodes the
apparatus required to produce hydrogen cyanide, the precise role of which in
bacterial biology remains to be established (ffrench-Constant and Waterfield
2006). P. entomophila has a large repertoire of potential virulence factors such as
insecticidal toxins, proteases, putative hemolysins, hydrogen cyanide, and
novel secondary metabolites that are envisaged to be important for virulence
toward insects. The two-component regulatory system GacS/GacA was observed
to play a key role in P. entomophila pathogenicity by regulating the expression of
many virulence factors (Vallet-Gely et al. 2010).
P. fluorescens produces a proteinaceous insecticidal toxin Fit that demonstrated
hemocoel-based toxicity in M. sexta and G. mellonella (Pechy-Tarr et al. 2008) and
caused complete loss of turgor pressure and melanization. Fit has been shown to be
orally toxic to S. littoralis, H. virescens, and P. xylostella. P. chlororaphis also
expresses a Fit toxin and has oral insecticidal activity (Ruffner et al. 2013). Certain
strains of P. fluorescens contain genes encoding δ-endotoxins, Mcf toxins, tc genes
encoding B and C components only, lipases, and exotoxins with hemolytic activity.
The genome of P. syringae pv. syringae contains an intact toxin complex encoding
ABC complement, suggesting that this species which was formerly thought to be
only plant pathogenic may have an association with insects (ffrench-Constant and
Waterfield 2006).
5.2.4 Insect Virulence Factors Produced by Serratia Species
Serratia spp. (Enterobacteriaceae) are commonly isolated from grassland soils, and
they often exist as endophytic rhizobacteria possessing antifungal activity. How-
ever, several species within the genus Serratia are often found associated with
insects of many orders (Grimont and Grimont 1978; Lamelas et al. 2011) and
nematodes (Rae et al. 2008; Abebe et al. 2011) in a facultative manner.
S. plymuthica, isolated from the intestine of Neombius fasciatus (Steinhaus 1941)
caused no infection in the insect host; however, S. marcescens and S. liquefaciens
were regarded as facultative pathogens. S. marcescens was found to infect lepidop-
teran hosts such as poorly reared H. virescens (Sikorowski et al. 2001). Contrarily,
S. entomophila and S. proteamaculans, the causal agents of amber disease of the

New Zealand grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), are
considered as true entomopathogenic bacteria (Jackson et al. 2001).
S. entomophila and S. proteamaculans colonize and propagate in the grass grub
crop; consequently, the infected larvae cease to feed, clear their gut, and become
amber-colored (Jackson et al. 1993; Jackson 1995). Eventually the bacteria invade
the hemolymph, causing general septicemia and death (Nuñez-Valdez et al. 2008).
The pathogenicity determinants in S. entomophila are encoded at two regions on a
153-kb plasmid designated as pADAP (amber disease-associated plasmid). The afp
(anti-feeding prophage) gene cluster, encoding an R-type pyocin structure, medi-
ates the transport of toxins to a target site and causes a cessation of feeding by the
grass grub larvae (Hurst et al. 2004; 2007a). The sep virulence-associated region
which comprises of three genes designated as sepA (tcdA-like), sepB (tcdB-like),
and sepC (tccC-like) mediate for the amber disease symptoms of gut clearance and
amber coloration of the larvae (Hurst et al. 2000). Both the sep genes and afp are
needed for full virulence of Serratia in grass grubs. Amber disease was found to be
chronic in nature and despite widespread testing; no other insect species have been
shown to be susceptible to S. entomophila. The host-specific nature of insecticidal
proteins from Serratia therefore limits their use in crop protection as these proteins
can be deployed against only specific species of pest insects. The sepABC genes
show homology to the components of the Tc proteins from P. luminescens (Bowen
et al. 1998) and xpt genes identified from X. nematophilus (Morgan et al. 2001).
However, while Tc toxins have shown cytotoxic effects, direct toxicity of the
Sep proteins is unknown. Sep gene orthologues were found to be plasmid-borne
in S. entomophila, S. proteamaculans, S. liquefaciens, and Yersinia frederiksenii.
The sepA and sepB genes of S. entomophila show high nucleotide identity to
Y. frederiksenii genes thus suggesting a horizontal gene transfer between the two
species (Dodd et al. 2006).
5.2.5 Insect Virulence Factors Produced by Yersinia Species
Members of the genus Yersinia (Enterobacteriaceae) have undergone extensive

diversification during the course of their evolution and are represented by patho-
genic species such as Y. pestis and Yersinia ruckeri, the causative agent of bubonic
plague (Perry and Fetherston 1997) and the causative agent of enteric redmouth
disease in salmonid fish, respectively (Ewing et al. 1978), while other species (e.g.,
Y. aldovae) have diverged into nonpathogenic organisms (Sulakvelidze 2000).
Y. entomophaga is a non-sporulating entomopathogenic bacterium that was isolated
from diseased larvae of the New Zealand grass grub, Costelytra zealandica White
(Coleoptera: Scarabaeidae) (Hurst et al. 2011a). The pathogenicity island of
Y. entomophaga, termed PAIYe96, is composed of the multi-subunit toxin complex
(Yen-Tc) showing homology with toxin complexes produced by Photorhabdus spp.
and insecticidal chitinases. The Tc complex includes three protein families termed
A (YenA1, Yen A2), B (YenB), and C (YenC1, YenC2) and two chitinases (Chi1
and Chi2) with high endochitinase activity (Hurst et al. 2011b). The 3D structures
of the Tc complex showed that subunits YenA1 and YenA2 form the basis of a
fivefold symmetric assembly, while subunits B and C form a surface accessible
region and are the main toxicity determinants. The structure of the chitinases that
adorn the surface of the TcA scaffold has been analyzed and different hypothesis
have been proposed to explain their role in mediating Tc toxicity (Landsberg
et al. 2011; Busby et al. 2012). Tc protein complex from Y. entomophaga exhibits
broad host range oral insecticidal activity, causing rapid mortality in many insect
pests belonging to the orders Coleoptera, Lepidoptera, and Orthoptera. The culture
supernatants of Y. entomophaga were found to be toxic to a variety of coleopteran
species, including the New Zealand grass grub, C. zealandica (Coleoptera:
Scarabaeidae); the redheaded cockchafer, Adoryphorus couloni (Coleoptera:
Scarabaeidae); the blackheaded pasture cockchafer, Acrossidius tasmaniae (Cole-
optera: Scarabaeidae); and the diamondback moth, P. xylostella (Lepidoptera:
Plutellidae) (Hurst et al. 2011b; Castagnola and Stock 2014). In P. xylostella, initial
apical swelling of gut columnar cells occurred after ingestion of purified Tc from
Y. entomophaga, followed by complete dissolution of the gut lining (Hurst
et al. 2011b). The orally active nature and the broad-spectrum insecticidal activity
of the Tc protein derived from Yersinia species indicates that it may be a potential
alternative to B. thuringiensis toxins for use in insect control (Bravo and Soberon
2008).
5.3 Toxins Shared by Gram-Negative Bacterial Pathogens

of Invertebrates: New Insights from the Comparative
Genomics of Entomopathogens
The proliferation of genomic information of invertebrate pathogens (P. luminescens,

X. nematophilus, P. entomophila, etc.) and functional analysis of genome data has
revealed that the composition of bacterial genomes is dynamic and susceptible to
many changes through the process of genome reduction (Moran 2002), gene dupli-
cation and divergence (Ohno 1970), vertical inheritance (Woese 1987), and hori-
zontal gene transfer (Ochman et al. 2000) that occur due to multiple pressures,
including the environment, mutation, and competition (Chaston et al. 2011). There-
fore, comparative genomics will provide an excellent opportunity to elucidate the
genetic similarities and differences in different species that will have an impact on
the innovations in crop protection technologies, in addition to providing a funda-
mental understanding of evolutionary relationships and changes contributing to
pathogenesis in bacteria. Bacterial genome can be subdivided into “core genome”
and “flexible genome.” Core genome of bacterial communities is common to all
bacterial strains in a defined set of species and contributes to basic cellular functions.
The genomic regions variably present between individual strains constitute “flexible
genome” component, and these regions are organized principally into polymorphic
strain-specific segments called regions of genome plasticity (RGPs) that play an
important role in bacterial adaptation to special growth conditions, such as those
involved in the colonization of new ecological niches, symbiosis, host-cell interac-
tion, and pathogenicity. Flexible gene pools act as a site for inter-genomic and intra-
genomic rearrangements (Frost et al. 2005; Gaudriault et al. 2008). RGPs
(underlining the continuous gene transfer among the bacterial genomes), insertion
sequences (IS), putative transposons, and the presence of phage remnants are the key
indicators of the transfer of genetic elements among different microbes, especially in
the bacterial genomes. The flexible genome of the Xenorhabdus and Photorhabdus
genera accounted for 52.6 to 61.5 % of the entire genome, and this region was found
to be larger in Photorhabdus than in Xenorhabdus (Ogier et al. 2010).
A comparison between entomopathogenic bacteria in the genera Photorhabdus
and Xenorhabdus revealed that despite their similar lifestyles the species within the
two genera use functionally different approaches to achieve successful host inter-
actions. Photorhabdus spp. encode a dedicated type three secretion system (TTSS)
which can suppress phagocytosis and nodule formation by injection of effectors,
such as LopT and SctC directly into hemocyte cells (Brugirard-Ricaud et al. 2004;
Brugirard-Ricaud et al. 2005). Xenorhabdus spp. lack a TTSS and associated
effectors; however, they do encode several other cytotoxic strategies in order to
evade the host cellular responses (Hinchliffe et al. 2010). The TTSS of
Photorhabdus is highly similar to the plasmid encoded system of Y. pestis (Wolters
et al. 2013). However, in Yersinia, the effector protein YopT is a cytotoxic cysteine
protease, whereas the homolog in P. luminescens, called LopT, has been shown to
prevent phagocytosis (Brugirard-Ricaud et al. 2004).
Insecticidal toxin complexes (Tc’s) were first identified in P. luminescens and
have been studied extensively by independent research groups (Bowen et al. 1998;
ffrench-Constant et al. 2000, 2003). Tc toxins were subsequently identified in the
genomes of other gram-negative insect pathogens such as X. nematophila (Morgan
et al. 2001), S. entomophila (Hurst et al. 2007b), and Y. entomophaga (Hurst
et al. 2011b) and have even been reported in the gram-positive Paenibacillus
(Hinchliffe et al. 2010). The genome of Y. pestis contains a locus encoding the Tc
protein homologues yitA (TcaA-like), yitB (TcaB-like), and yitC (TcaC-like) and
YipA and YipB (two TccC-like proteins). The tcaB and tcaC genes of Y. pestis
contain a frame shift mutation and internal deletion, respectively, which is indi-
cative of a loss of function (Parkhill et al. 2001; Spinner et al. 2012). The nomen-
clature of the Tc proteins has been revised (ffrench-Constant and Waterfield 2005),
and the ABC designation is adopted currently to describe the components of the Tc
complex. The Tc-Bs and Tc-Cs are known to make the Tc-As more toxic. The tc-
like genes identified in P. luminescens, X. nematophila, and Y. pestis are chromo-
somally borne, while the sep genes of S. entomophila and tc-like genes of
Y. frederiksenii strain 49 are plasmid-borne. A toxin-encoding operon similar to the
tca of P. luminescens W14 was found to be present in an isolate of Bt (Bt-IBL200)
from the Invasive Insect Biocontrol and Behavior Laboratory (IIBBL, Beltsville,
MD, USA) (Blackburn et al. 2011). All three components of tc (A, B, and C) were
present in the Bt IBL200 isolate. The genomic organization and diversity of Tc
proteins among different species of insect-pathogenic bacteria increases the likeli-
hood of tc gene transfer between species via plasmids, and suggests that the chro-
mosomally located tc genes could also once have been plasmid-borne or mobile in
other bacteria (Dodd et al. 2006). Tc toxins are active against different tissues within
individual hosts, namely, Tcb against hemocytes and Tcd and Tca against cells of the
insect gut. The Tc toxins reside as multiple but dissimilar orthologues throughout the
P. luminescens TT01 genome with different insecticidal activities attributed to a
different Tc cluster (Duchaud et al. 2003; Hey et al. 2006). Plasmid-borne Sep
proteins of S. entomophila are host-specific. The xpt genes of Xenorhabdus exist on
a PAI like the tc genes of Photorhabdus; however, PAIs are nearly identical in
Xenorhabdus. The insecticidal genes tcdB1, yitC, and spvB of P. luminescens,
Y. pestis, and Salmonella, respectively, have regions of homology, viz., the
N-terminal 367 amino acids of yitC are similar to the N-terminus of the putative
effector spvB of Salmonella (Browne et al. 2002; Castagnola and Stock 2014). The
Yersinia spp. contain islands harboring insecticidal tc-like genes; however, insecti-
cidal activity was observed only when a low-growth temperature was used to culture
the bacteria and produce a protein extract (Bresolin et al. 2006).
Chitinases having antimycotic activity have been found in both Xenorhabdus
and Photorhabdus (Chen et al. 1996). Interestingly, the assembled Tc toxin of
Y. entomophaga was found to have endochitinase activity, which was attributed to
putative chitinase subunits associated with TcA scaffold. This has not previously
been reported in a Tc (Hurst et al. 2011b; Landsberg et al. 2011). S. marcescens was
found to produce orally active insecticidal toxins and chitinases (Jeong et al. 2010;
Brurberg et al. 1996). S. marcescens cultures and Bt Cry1C toxin exhibited a
synergistic insecticidal effect against S. litura (Asano et al. 1999).
The apoptotic binary toxin Xax is found in P. luminescens and X. nematophila.
X. nematophila XaxA and XaxB showed the strongest similarity to plu3075 (61 %)
and plu1961 (56 %), respectively, from P. luminescens. The putative hemolysin
loci, containing two closely linked genes, xaxA and xaxB, are found together in
genome sequences from various bacterial pathogens of plants (P. syringae), insects
(Photorhabdus, Xenorhabdus, P. entomophila), and humans (P. mirabilis,
Y. enterocolitica). The xaxAB homologues in X. nematophila are found in a unique
genomic context that does not show characteristic features of genome flexibility,
such as genomic islands, transposon-related structures, or phages (Vigneux
et al. 2007). Interestingly, the xax hemolysin locus was found to be present in
Y. enterocolitica and not in Y. pestis even though the latter, like X. nematophila,
spends part of its life cycle in an insect.
The mcf (makes caterpillar floppy) gene, which encodes a large proapoptotic
multidomain protein, is present in P. luminescens, P. temperata, and P. asymbiotica
(Daborn et al. 2002; Forst and Goodner 2006). Two mcf paralogous genes (mcf1 and
mcf2) are found in the strains Pl W14 and Pl TT01. Genome sequencing has
revealed the presence of other Mcf-like proteins in other bacterial species. The
FitD gene of P. fluorescens encodes a Mcf1-like protein and shows 73.5 % identity
to P. luminescens mcf1 (Pechy-Tarr et al. 2008). The fitD locus of P. fluorescens is

associated with a TolC-family outer membrane efflux protein ( fitE), two response
regulators ( fitF, fitH), and a LysR-like regulator (fitG). However, paralogues of
fitE–H are not present in Photorhabdus and may be involved in the specific
regulation of fitD in P. fluorescens (Hinchliffe et al. 2010).
RTX, the repeats in toxin family possessing different enzymatic activities
including cytolytic, protease, or lipase activity are observed to be dramatically
expanded in P. luminescens (eight rtxA genes), but this gene has not been found in
Y. pestis. Four of the eight rtxA genes of P. luminescens were disrupted by either
insertion sequence (IS) elements or inactivated by frameshift mutations. An
RTX-like alkaline protease has recently been isolated from P. luminescens W14
and P. temperata (Bowen et al. 2003; Forst and Goodner 2006). Phage-related loci
are found in both Serratia and Photorhabdus. The PVCs of Photorhabdus are
homologous to a prophage-like locus on the pADAP plasmid of S. entomophila
(Yang et al. 2006). The PVCs have injectable toxicity toward G. mellonella hemo-
cytes, whereas the pADAP locus has been associated with anti-feeding effects
(Hurst et al. 2004). The txp40 gene, encoding a 42 kDa protein with injectable
cytotoxic activity, was identified in several strains of Xenorhabdus and
Photorhabdus, indicating that it is both highly conserved and widespread among
these bacteria (Brown et al. 2006). Proteins similar to the δ-endotoxin from
B. thuringiensis have also been identified in P. luminescens. The pir gene of
Photorhabdus shows a 30 % amino acid sequence similarity to part of the insecti-
cidal crystal protein of B. thuringiensis (Li et al. 2009).
The genome of P. luminescens was found to be ~1 Mb larger than closely related
bacteria such as Xenorhabdus and Yersinia spp. The average genome size of most
members of the Enterobacteriaceae family is approximately 4.6 Mb (Forst and
Goodner 2006). The emerging human pathogen P. asymbiotica has a smaller genome
than that of P. luminescens genome. A reduction in the genome size of P. asymbiotica
was found to coincide with a reduction in different classes of anti-insect virulence
factors. Unlike P.luminescens strains, the P. asymbiotica strains carry a plasmid
related to pMT1 from Y. pestis that promotes deep tissue invasion, and several
PAIs including a novel TTSS; these features suggested that human pathogenicity in
P. asymbiotica was acquired through the acquisition of pMT1-like plasmid and
specific effectors. Despite these molecular changes, the pathogenicity toward insects
was found to remain intact in P. asymbiotica illustrating a lifestyle superior to
P. luminescens, which is pathogenic only to insects (Wilkinson et al. 2009).
Comparative genome analysis, gene profiling, functional genomics, and the
newly developed genetic approaches like microarrays and software tools like
RGP finder will be helpful for several unresolved, mechanistic and evolutionary
questions about members of soil bacteria in future. Whole-genome sequencing
approaches and comprehensive analysis undertaken by the rapid virulence annota-
tion (RVA) technique (Waterfield et al. 2008) have begun to reveal previously
unidentified insecticidal toxins, uncharacterized secondary metabolites possessing
toxic activities, putative lipases, and type VI secretion systems with insecticidal
activity. Thus, a significant challenge of comparative genomics is to interpret the
interrelationships between anti-invertebrate and anti-vertebrate virulence factors
and assign biological roles to the candidate virulence factors (Hinchliffe

et al. 2010).
5.4 Strategies for Enhancing Transgenic Resistance

to Lepidopteran Pests: A Dynamic Landscape
Undoubtedly, B. thuringiensis and its insecticidal toxins have been overwhelmingly

successful for agronomical pest control for decades. Transgenic crops protected
from the damage of lepidopteran and coleopteran insect feeding express insecticidal
proteins derived from the entomopathogenic bacteria, B. thuringiensis (Huesing
and English 2004). The first generation of genetically modified (GM) crops con-
ferring insect protection has been extraordinarily successful, and GM crops are
considered as the fastest adopted crop technology in the history of modern agricul-
ture (James 2012). Insect-resistant products expressing Bt-derived proteins have
been available for corn and cotton since 1996. Bt cotton, in particular, has provided
effective control of several lepidopteran pest species including tobacco budworm,
H. virescens F. (Lepidoptera: Noctuidae); pink bollworm, P. gossypiella Saunders
(Lepidoptera: Gelechiidae); the cotton bollworm, H. armigera Hübner (Lepidop-
tera: Noctuidae); and Spodoptera spp. (Lepidoptera: Noctuidae) (Sanahuja
et al. 2011; Baum et al. 2012). The second-generation GM varieties generated by
stacking and pyramiding resistance genes were also approved for commercial-
ization (Marra et al. 2010). Bt cotton planted in more than 18.8 million hectares
in 13 countries was the third most dominant crop grown in 2012, which is equi-
valent to 11 % of the global biotech area (James 2012). Despite the commercial
success of the Bt technology, there are concerns over the development of resistance
by insect species, the problem of efficacy, and narrow spectrum of activity of Bt
proteins (de Maagd et al. 2001; Pereira et al. 2008; Sayyed et al. 2008). Therefore,
efforts are ongoing for the discovery of potent Bt strains expressing novel toxins
with improved and broad-spectrum activity as well as for the characterization of
genes exhibiting biopesticidal properties from other entomopathogens (Christou
et al. 2006; Crickmore 2006).
Whole-genome sequencing and screening of genomes of soil-dwelling gram-
negative entomopathogenic bacteria and gram-negative bacterial symbionts of soil-
dwelling EPNs has yielded a gold mine of potential novel insect toxins that
augment a growing list of candidates for use in crop protection (Hinchliffe
et al. 2010). The insect virulence factors from gram-negative bacteria that can be
used as biocontrol agents against Lepidoptera include a host of insecticidal toxins,
proteases, putative hemolysins, and other previously unidentified proteins.
Although a surge in the patent applications has been observed with the discovery,
functional annotation, and insecticidal efficacy evaluation of novel genes/proteins,
data on how far many of these potential candidates have progressed toward
commercial field applications is ambiguous (Hinchliffe et al. 2010).
Photorhabdus, Xenorhabdus, and other gram-negative entomopathogens have

provided novel candidates having both oral and hemolymph-based toxic activity.
Proteins that are active upon ingestion and damage the insect midgut are good
candidates for developing transgenic crops. The “toxin complexes” are a prime
candidate fulfilling these criteria. However, the orally toxic nature of the Tc pro-
teins produced by an insect pathogen that is directly delivered into the insect
hemolymph is intriguing. The presence of tc genes in many organisms which are
not directly delivered into the insect hemolymph may somewhat explain the oral
toxicity of Tc proteins (Castagnola and Stock 2014). The histopathological effects
on lepidopteran larvae of toxins that include Tc proteins, the mcf gene product, the
PirAB binary toxins, the 17 kDa pilin subunit, and the Txp40 proteins from
Photorhabdus and Xenorhabdus demonstrated a distinct damage to the midgut.
The tc genes have been studied extensively by researchers in academia and industry
and were considered suitable for commercial product development. One of the Tc
proteins, the tcdA protein expressed in A. thaliana to sufficient levels, was found to
be toxic to M. sexta (Liu et al. 2003). The demonstration that the large toxin genes
such as tc can be engineered and expressed in transgenic plants makes them suitable
alternative toxins to B. thuringiensis (Schnepf et al. 1998). Application of Pir toxins
in insect control may be limited, as they were found to be effective only against the
diamondback moth, P. xylostella. The limited activity spectrum and the possible
relation of the Pir toxins to the leptinotarsins may indicate problems with vertebrate
toxicity. Toxins like Mcf which act on both the gut and insect immune system
represent a promising, yet underexploited avenue for the development of insect-
resistant crops in the future (Daborn et al. 2002). It can be speculated that there are
many more toxins yet to be functionally characterized from the Photorhabdus
genome which are responsible for hemolymph-based toxicity.
Stacking virulence factors like Mcf which have hemolymph-based toxicity with
conventional orally active toxins would expand their application to IPM. However,
only those hemolymph-based toxins which are toxic upon ingestion as well could
be used in this case, as these proteins have to be stable in the insect gut. Alter-
natively, two proteins with different modes of action and diverse targets can also be
used in developing novel combinations of genes for pest control, as it has the
advantage of reducing the development of insect resistance (Gould 1998). Site-
directed mutagenesis and domain-engineering have great potential to alter toxin-
encoding genes particularly when sufficient structural information is available,
these methods can thus be applied to produce novel recombinant toxins (Gatehouse
2008). Using a combination of insect-toxic genes derived from B. thuringiensis and
gram-negative entomopathogens can also result in synergistic insecticidal activity
(Asano et al. 1999).
The ability of P. entomophila to orally infect and kill larvae of insect species
belonging to different orders makes it a promising model for the study of host–
pathogen interactions and for the development of biocontrol agents against insect
pests. S. entomophila was developed as a biopesticide and used for 15 years as a
commercial product for grass grub control in New Zealand. This microbe was
initially developed and applied as a liquid biopesticide Invade®, which is
New Zealand’s first registered, safety-tested, indigenous biological pesticide and
the first microbial control agent in the world to be based on a member of the gram-
negative Enterobacteriaceae (Jackson et al. 1992). The pathogenic bacterium has
recently been developed for application as a solid granule formulation, Bioshield™
(Young et al. 2010).
The important attributes of an insecticidal protein for successful commercial use
include its efficacy and specificity. First and foremost the insecticidal toxin must be
highly toxic to a wide range of potential pests, and it has to prevent crop damage by
efficiently killing or deterring the insect pests. Delivery of toxic proteins to poten-
tial pests is either through their host plants via the expression of the toxin, or toxic
subunit/domain, in transgenic crops or developing a pesticide formulation and
coating them onto the crops in a stable form (Hinchliffe et al. 2010; Ruiu
et al. 2013). Due to specific biological properties and technical reasons such as
the specific mode of action (oral or injectable activity), target site of action, and
stability, commercially available strains including Bt have their restrictions in terms
of performance in the field. The toxin protein has to be orally active for developing
insect-resistant GM crops. Although “toxin complex” proteins described in differ-
ent gram-negative entomopathogens are orally active, the Tc’s consist of large
protein subunits which are inherently difficult to express transgenically in crop
plants. Therefore, only individual proteins having limited toxicity can be expressed
with the technology available currently. As both toxins and potentiators have to be
co-expressed in order to harness the full toxic potential of tc genes, either the
proteins have to be cropped down to smaller active domains or the transgenic
technology has to be improved to allow all subunits to be expressed to achieve
the desired level of efficacy (Hinchliffe et al. 2010). Transgenic expression of
ingestible insecticidal proteins confers certain degree of pest specificity as only
insects actually feeding on the crop will ingest the toxin directly. The toxicity of
insecticidal Tc proteins of P. luminescens to cultured mammalian cells may attract
criticism particularly with regard to their use in crop protection and the associated
concerns on biosafety (Waterfield et al. 2005a; Hares et al. 2008). The
bioecological compatibility of P. luminescens biopesticide against two species of
the beneficial insect Trichogramma was investigated by Mohan and Sabir (2005).
Their study demonstrated that there was a significant reduction of up to 84 % in the
emergence of Trichogramma adults from the host, Corcyra cephalonica eggs, as
65 % of the eggs exposed to either P. luminescens cells or their toxins became
flaccid. In some species of pathogenic yersiniae viz., Yersinia pseudotuberculosis
and Yersinia pestis the tc genes are not insecticidal but have evolved to show
mammalian pathogenicity. These data suggest that the biological activity of the
toxins against target species and nontarget species should be thoroughly investi-
gated before being considered for crop protection. However, appropriate evaluation
of the effects of the toxin on the target pests should be undertaken so that the protein
is active toward the target species and does not cause any harm to the non-pest
species (beneficial insects, bystander insect species, predatory species which feed
off the intoxicated pests, humans) upon exposure.
5.5 Conclusion and Perspectives
Insecticidal toxins are an important option for the biological control of lepidopteran
insect pests. Their use in genetic engineering of plants could provide a new
generation of insect-resistant crops that can help in maintaining crop yields. A
majority of the toxic proteins expressed by gram-negative bacteria have been tested
only against model insects (M. sexta, G. mellonella, S. litura, P. xylostella,
H. armigera), and besides few reports on mammalian toxicity, there is very limited
information on the effects of these proteins on beneficial fauna (predators, para-
sitoids, and pollinators). Therefore, a significant research need is centered on
understanding the specific effects of the insecticidal proteins, their activity spec-
trum, and their effect on nontarget organisms in the ecological sphere.
Insect pest control has entered the genomic era with the recent sequencing and
functional analysis of the genomes of agricultural pests and entomopathogenic
bacteria. This has enabled the discovery of novel targets in the pests and novel
proteins in the entomopathogens and has provided a comprehensive understanding
of invertebrate pathology by providing critical insights into evolutionary patterns of
bacterial pathogens. Genome analysis has also raised several pertinent questions
about the complex life cycles of these pathogens and their association with various
invertebrate hosts and vectors. Ultimately a relevant challenge of comparative
genomics is to understand the interrelationships between pathogenic mechanisms
targeted to invertebrates and vertebrates as such insight may help us understand the
evolution and the probable invertebrate origins, of emerging human pathogens,
in addition to using the insecticidal genes derived from these bacteria for large-
scale, commercial agricultural applications.
References
Abebe E, Abebe-Akele F, Morrison J (2011) An insect pathogenic symbiosis between a

caenorhabditis and serratia. Virulence 2:158–161
Akhurst RJ (1982) Antibiotic activity of Xenorhabdus spp, bacteria symbiotically associated with
insect pathogenic nematodes of the families Heterorhabditidae and Steinernematidae. J Gen
Microbiol 128:3061–3065
Akhurst RJ (1983) Taxonomic study of Xenorhabdus, a genus of bacteria symbiotically associated
with insect pathogenic nematodes. Int J Syst Bacteriol 33:38–45
Asano S, Suzuki K, Hori H (1999) Synergistic effects of the supernatants from Serratia
marcescens culture on larvicidal activity of Bacillus thuringiensis Cry1C toxin against com-
mon cutworm, Spodoptera litura. J Pestic Sci 24:44–48
Baum JA, Sukuru UR, Penn SR, Meyer SE, Subbarao S, Shi X, Flasinski S, Heck GR, Brown RS,
Clark TL (2012) Cotton plants expressing a hemipteran-active Bacillus thuringiensis crystal
protein impact the development and survival of Lygus hesperus (Hemiptera: Miridae) nymphs.
J Econ Entomol 105:616–624
Bedding RA, Akhurst RJ, Kaya HK (1993) Nematodes and the biological control of insect pests.
CSIRO Publications, Melbourne, p 178
Beveridge TJ (1999) Structures of gram-negative cell walls and their derived membrane vesicles.
J Bacteriol 181:4725–4733
Blackburn M, Golubeva E, Bowen D, ffrench-Constant RH (1998) A novel insecticidal toxin from
Photorhabdus luminescens, Toxin Complex a (Tca), and its histopathological effects on the
midgut of Manduca sexta. Appl Environ Microbiol 64:3036–3041
Blackburn MB, Farrar RR, Novak NG (2006) Remarkable susceptibility to the diamondback moth
(Plutella xylostella) to ingestion of Pir toxins from Photorhabdus luminescens. Entomol Exp
Appl 121:31–37
Blackburn MB, Martin PA, Kuhar D, Farrar RR Jr, Gundersen-Rindal DE (2011) The occurrence
of Photorhabdus-like toxin complexes in Bacillus thuringiensis. PLoS One 6. doi:10.1371/
journal.pone.0018122
Bonning BC, Hammock BD (1996) Development of recombinant baculoviruses for insect control.
Bowen DJ, Ensign JC (1998) Purification and characterization of a high-molecular-weight insec-
ticidal protein complex produced by the entomopathogenic bacterium Photorhabdus
Bowen D, Rocheleau TA, Blackburn M, Andreev O, Golubeva E, Bhartia R, ffrench-Constant RH
(1998) Insecticidal toxins from the bacterium Photorhabdus luminescens. Science 280:
2129–2132
Bowen D, Rocheleau TA, Grutzmacher CK, Meslet L, Valens LM, Marble D, Dowling D, ffrench-
Constant RH, Blight MA (2003) Genetic and biochemical characterization of PrtA, an
RTX-like metalloprotease from Photorhabdus. Microbiology 149:1581–1591
Bravo A, Soberon M (2008) How to cope with insect resistance to Bt toxins? Trends Biotechnol
26:573–579
Bresolin G, Morgan JA, Illgen D (2006) Low temperature-induced insecticidal activity of
Yersinia enterocolitica. Mol Microbiol 59:503–512
Brillard J, Ribeiro C, Boemare N, Brehélin M, Givaudan A (2001) Two distinct hemolytic
activities in Xenorhabdus nematophila are active against immunocompetent insect cells.
Brillard J, Duchaud E, Boemare N, Kunst F, Givaudan A (2002) The PhlA hemolysin from the
entomopathogenic bacterium Photorhabdus luminescens belongs to the two-partner secretion
family of hemolysins. J Bacteriol 184:3871–3878
Brooke E, Hines E (1999) Viral biopesticides for Heliothine control-fact of fiction? Today’s Life
Sci 11:38–45
Brown SE, Cao AT, Hines ER, Akhurst RJ, East PD (2004) A novel secreted protein toxin from the
insect pathogenic bacterium Xenorhabdus nematophila. J Biol Chem 279:14595–14601
Brown SE, Cao AT, Dobson P, Hines ER, Akhurst RJ, East PD (2006) Txp40, a ubiquitous
insecticidal toxin protein from Xenorhabdus and Photorhabdus bacteria. Appl Environ
Browne SH, Lesnick ML, Guiney DG (2002) Genetic requirements for salmonella-induced
cytopathology in human monocyte-derived macrophages. Infect Immun 70:7126–7135
Brugirard-Ricaud K, Givaudan A, Parkhill J, Boemare N, Zumbihl R, Duchaud E (2004) Variation
in the effectors of the Photorhabdus type III secretion system among species revealed by
genomic analysis. J Bacteriol 186:4376–4381
Brugirard-Ricaud K, Duchaud E, Givaudan A, Girard PA, Kunst F, Boemare N, Brehelin M,
Zumbihl R (2005) Site-specific antiphagocytic function of the Photorhabdus luminescens type
III secretion system during insect colonization. Cell Microbiol 7:363–371
Brurberg MB, Nes IF, Eijsink VG (1996) Comparative studies of chitinases A and B from
Serratia marcescens. Microbiology 142:1581–1589
Budd RC (2001) Activation-induced cell death. Curr Opin Immunol 13:356–362
Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P (2001) Structure of the Rho-activating domain of
Escherichia coli cytotoxic necrotizing factor 1. Nat Struct Biol 8:584–588
Busby JN, Landsberg MJ, Simpson R, Jones SA, Hankamer B, Hurst MRH, Lott JS (2012)
Structural analysis of Chi1 chitinase from Yen-Tc: The multisubunit insecticidal ABC toxin
complex of Yersinia entomophaga. J Mol Biol 415:359–371
Carrière Y, Ellers-Kirk C, Sisterson M, Antilla L, Whitlow M, Dennehy TJ, Tabashnik BE (2003)
Long-term regional suppression of pink bollworm by Bacillus thuringiensis cotton. Proc Natl
Acad Sci USA 100:1519–1523
Carson R (1962) Silent spring. Houghton, Mifflin
Castagnola A, Stock P (2014) Common virulence factors and tissue targets of entomopathogenic
bacteria for biological control of lepidopteran pests. Insects 5:139–166. doi:10.3390/
insects5010139
Chaston JM, Suen G, Tucker SL, Andersen AW, Bhasin A, Bode E, Bode HB, Brachmann AO,
Cowles CE, Cowles KN, Darby C, de Léon L, Drace K, Du Z, Givaudan A, Herbert Tran EE,
Jewell KA, Knack JJ, Krasomil-Osterfeld KC, Kukor R, Lanois A, Latreille P, Leimgruber NK,
Lipke CM, Liu R, Lu X, Martens EC, Marri PR, Médigue C, Menard ML, Miller NM, Morales-
Soto N, Norton S, Ogier JC, Orchard SS, Park D, Park Y, Qurollo BA, Sugar DR, Richards GR,
Rouy Z, Slominski B, Slominski K, Snyder H, Tjaden BC, van der Hoeven R, Welch RD,
Wheeler C, Xiang B, Barbazuk B, Gaudriault S, Goodner B, Slater SC, Forst S, Goldman BS,
Goodrich-Blair H (2011) The entomopathogenic bacterial endosymbionts Xenorhabdus and
Photorhabdus: convergent lifestyles from divergent genomes. PLoS One 6:e27909
Chen G, Zhang Y, Li J (1996) Chitinase activity of Xenorhabdus and Photorhabdus species,
bacterial associates of entomopathogenic nematodes. J Invertebr Pathol 68:101–108
Christou P, Capell T, Kohli A, Gatehouse JA, Gatehouse AM (2006) Recent developments and
future prospects in insect pest control in transgenic crops. Trends Plant Sci 11:302–308
Cowles KN, Goodrich-Blair H (2005) Expression and activity of a Xenorhabdus nematophila
haemolysin required for full virulence towards Manduca sexta insects. Cell Microbiol 7:
209–219
Crickmore N (2006) Beyond the spore – past and future developments of Bacillus thuringiensis as
a biopesticide. J Appl Microbiol 101:616–619
Crosland RD, Fitch RW, Hines HB (2005) Characterization of β-leptinotarsin-h and the effects of
calcium flux agonists on its activity. Toxicon 45:829–841
da Silva CC, Dunphy GB, Rau ME (2000) Interaction of Xenorhabdus nematophilus (Entero-
bacteriaceae) with the antimicrobial defenses of the house cricket, Acheta domesticus.
Daborn PJ, Waterfield N, Silva CP (2002) A single Photorhabdus gene, makes caterpillars floppy
(mcf), allows Escherichia coli to persist within and kill Insects. Proc Natl Acad Sci USA 99:
10742–10747
de Maagd RA, Bravo A, Crickmore N (2001) How Bacillus thuringiensis has evolved specific
toxins to colonize the insect world. Trends Genet 17:193–199
Dodd SJ, Hurst MR, Glare TR, O’Callaghan M, Ronson CW (2006) Occurrence of sep insecticidal
toxin complex genes in Serratia spp. and Yersinia frederiksenii. Appl Environ Microbiol 72:
6584–6592
Dowling AJ, Daborn PJ, Waterfield NR, Wang P, Streuli CH, ffrench-Constant RH (2004) The
insecticidal toxin Makes caterpillars floppy (Mcf) promotes apoptosis in mammalian cells.
Cell Microbiol 6:345–353
Duchaud E, Rusniok C, Frangeul L, Buchrieser C, Givaudan A, Taourit S, Bocs S, Boursaux-
Eude C, Chandler M, Charles JF, Dassa E, Derose R, Derzelle S, Freyssinet G, Gaudriault S,
Medigue C, Lanois A, Powell K, Siguier P, Vincent R, Wingate V, Zouine M, Glaser P,
Boemare N, Danchin A, Kunst F (2003) The genome sequence of the entomopathogenic
bacterium Photorhabdus luminescens. Nat Biotechnol 21:1307–1313
Ewing WH, Ross AJ, Brenner DJ, Fanning GR (1978) Yersinia ruckeri sp. nov., the Redmouth
(RM) Bacterium. Int J Syst Bacteriol 28:37–44. doi:10.1099/00207713-28-1-37
Federici BA, Siegel JP (2008) Safety assessment of Bacillus thuringiensis and Bt crops used in
insect control. In: Hammond BG (ed) Food safety of proteins in agricultural biotechnology.
CRC Press Taylor and Francis Group, Chapter 3, pp 45–102
ffrench-Constant RH, Waterfield NR (2005) An ABC guide to the bacterial toxin complexes.
Adv Appl Microbiol 58:169–183
ffrench-Constant RH, Waterfield NR (2006) Ground control for insect pests. Nat Biotechnol
24:660–661
ffrench-Constant RH, Waterfield N, Burland V, Perna NT, Daborn PJ, Bowen D, Blattner FR
(2000) A Genomic sample sequence of the entomopathogenic bacterium Photorhabdus
luminescens W14: Potential implications for virulence. Appl Environ Microbiol 66:3310–3329
ffrench-Constant RH, Waterfield NR, Daborn P, Joyce S, Bennet H, Au C, Dowling A, Boundy S,
Reynolds S, Clarke D (2003) Photorhabdus: towards a functional genome analysis of a
symbiont and pathogen. FEMS Microbiology Rev 26:433–456
Fischer-Le Saux M, Viallard V, Brunel B, Normand P, Boemare N (1999) Polyphasic classifi-
cation of the genus Photorhabdus and proposal of new taxa: P. luminescens subsp. luminescens
subsp. nov, P. luminescens subsp. akhurstii subsp. nov, P. luminescens subsp. laumondii subsp.
nov, P. temperata sp. nov, P. temperata subsp. temperata subsp. nov. and P. asymbiotica
sp. nov. Int J Syst Bacteriol 49:1645–1656
Fitt GP (1994) Cotton pest management: part 3; an Australian perspective. Annu Rev Entomol 39:
532–562
Forst S, Goodner B (2006) Comparative bacterial genomics and its use in undergraduate education.
Biol Control 38:47–53
Forst S, Nealson K (1996) Molecular biology of the symbiotic-pathogenic bacteria Xenorhabdus
spp. and Photorhabdus spp. Microbiol Rev 60:21–43
Frost LS, Leplae R, Summers AO, Toussaint A (2005) Mobile genetic elements: the agents of
open source evolution. Nat Rev Microbiol 3:722–732
Gassmann AJ, Petzold-Maxwell JL, Keweshan RS, Dunbar MW (2011) Field-evolved resistance
to Bt maize by western corn rootworm. PLoS One 6:e22629. doi:10.1371/journal.pone.
0022629
Gatehouse JA (2008) Biotechnological prospects for engineering insect-resistant plants.
Plant Physiol 146:881–887
Gatehouse AM, Hilder VA, Powell KS, Wang M, Davison GM, Gatehouse LN, Down RE,
Edmonds HS, Boulter D, Newell CA et al (1994) Insect-resistant transgenic plants:
choosing the gene to do the ‘job’. Biochem Soc Trans 22:944–949
Gaudriault S, Pages S, Lanois A, Laroui C, Teyssier C, Jumas-Bilak E, Givaudan A (2008) Plastic
architecture of bacterial genome revealed by comparative genomics of Photorhabdus variants.
Genome Biol 9:R117. doi:10.1186/gb-2008-9-7-r117
Gerrard J, Waterfield N, Vohra R, ffrench-Constant RH (2004) Human infection with
Photorhabdus asymbiotica: an emerging bacterial pathogen. Microbes Infect 6:229–237
Gerrard JG, Joyce SA, Waterfield NR (2006) Nematode symbiont for Photorhabdus asymbiotica.
Emerg Infect Dis 12:1562–1564
Gillespie JP, Kanost MR, Trenczek T (1997) Biological mediators of insect immunity. Ann Rev
Entomol 42:611–643
Goodrich-Blair H, Clarke DJ (2007) Mutualism and pathogenesis in Xenorhabdus and
Photorhabdus: two roads to the same destination. Mol Microbiol 64:260–268
Gould F (1998) Sustainability of transgenic insecticidal cultivars: integrating pest genetics and
ecology. Annu Rev Entomol 43:701–726
Griffin CT, O’ Callaghan K, Dix I (2001) A self-fertile species of Steinernema from Indonesia:
further evidence of convergent evolution amongst entomopathogenic nematodes? Parasitology
122:181–186
Grimont PAD, Grimont F (1978) The genus Serratia. Annu Rev Microbiol 32:221–248. doi:10.
1146/annurev.mi.32.100178.001253
Hacker J, Kaper JB (2000) Pathogenicity islands and the evolution of microbes. Annu Rev
Haq SK, Atif SM, Khan RH (2004) Protein proteinase inhibitor genes in combat against insects,
pests, and pathogens: natural and engineered phytoprotection. Arch Biochem Biophys
431:145–159
Hares MC, Hinchliffe SJ, Strong PC, Eleftherianos I, Dowling AJ, ffrench-Constant RH,
Waterfield N (2008) The Yersinia pseudotuberculosis and Yersinia pestis toxin complex is
active against cultured mammalian cells. Microbiology 154:3503–3517
Head G, Moar W, Eubanks M, Freeman B, Ruberson J, Hagerty A, Turnipseed S (2005) A
multiyear, large-scale comparison of arthropod populations on commercially managed Bt
and non-Bt cotton fields. Environ Entomol 34:1257–1266
Hey TD, Meade T, Burton SL, Merlo DJ, Cai Q, Moon HJ, Sheets JJ, Woosley AT (2006)
Insecticidal toxin complex fusion proteins. US Patent 2006/0168683
Hinchliffe SJ, Hares MC, Dowling AJ, ffrench-Constant RH (2010) Insecticidal toxins from the
Photorhabdus and Xenorhabdus bacteria. The Open Toxicol J 3:101–118
Hsiao TH, Fraenkel G (1969) Properties of leptinotarsin, a toxic hemolymph protein from the
Colorado potato beetle. Toxicon 7:119–130
Huang DF, Zhang J, Song FP, Lang ZH (2007) Microbial control and biotechnology research on
Bacillus thuringiensis in China. J Invertebr Pathol 95:175–180
Huesing J, English L (2004) The impact of crops on the developing world. AgBio Forum 7:84–95
Hurst MR, Glare TR, Jackson TA, Ronson CW (2000) Plasmid-located pathogenicity determi-
nants of Serratia entomophila, the causal agent of amber disease of grass grub, show similarity
to the insecticidal toxins of Photorhabdus luminescens. J Bacteriol 182:5127–5138
Hurst MR, Glare TR, Jackson TA (2004) Cloning Serratia entomophila antifeeding genes-a
putative defective prophage active against the grass grub Costelytra zealandica. J Bacteriol
186:5116–5128
Hurst MR, Beard SS, Jackson TA, Jones SM (2007a) Purification and characterisation of the
Serratia entomophila Afp. FEMS Microbiol Lett 270:42–48
Hurst MR, Jones SM, Tan B, Jackson TA (2007b) Induced expression of the Serratia entomophila
Sep proteins shows activity towards the larvae of the New Zealand grass grub Costelytra
zealandica. FEMS Microbiol Lett 275:160–167
Hurst MR, Becher SA, Young SD, Nelson TL, Glare TR (2011a) Yersinia entomophaga sp nov.,
isolated from the New Zealand grass grub Costelytra zealandica. Int J Syst Evol Microbial 61:
844–849
Hurst MR, Jones SA, Binglin T, Harper LA, Jackson TA, Glare TR (2011b) The main virulence
determinant of Yersinia entomophaga MH96 is a broad-host-range toxin complex active
against insects. J Bacteriol 193:1966–1980
Jackson TA (1995) Amber disease reduces trypsin activity in midgut of Costelytra zealandica
(Coleoptera, Scarabaeidae) larvae. Research note. J Invertebr Pathol 65:68–69
Jackson TA, Pearson JF, O’Callaghan M, Mahanty HK, Willocks M (1992) Pathogen to product –
development of Serratia entomophila (Enterobacteriaceae) as a commercial biological control
agent for the New Zealand grass grub (Costelytra zealandica). In: Glare TR, Jackson TA (eds)
Use of pathogens in scarab pest management. Intercept Ltd., Andover, pp 191–198
Jackson TA, Huger AM, Glare TR (1993) Pathology of amber disease in the New Zealand grass
grub Costelytra zealandica (Coleoptera: Scarabaeidae). J Invertebr Pathol 61:123–130
Jackson TA, Boucias DG, Thaler JO (2001) Pathobiology of amber disease, caused by Serratia
spp, in the New Zealand grass grub, Costelytra zealandica. J Invertebr Pathol 78:232–243
Jackson RE, Bradley JR, Van DWJ (2003) Field performance of transgenic cotton expressing one
or two Bacillus thuringiensis endotoxins against bollworm, Helicoverpa zea (Boddie). J Cotton
Sci 7:57–64
James C (2010) Global status of commercialized biotech/GMCrops ISAAA Briefs No. 42.
ISAAA, Ithaca
James C (2012) Global status of commercialized biotech/GM Crops: 2012. ISAAA Briefs No. 44.
ISAAA, Ithaca
Jeong HU, Mun HY, Oh HK (2010) Evaluation of insecticidal activity of a bacterial strain
Serratia, sp EML-SE1 against diamondback moth. J Microbiol 48:541–545
Joshi MC, Sharma A, Kant S, Birah A, Gupta GP, Khan SR, Bhatnagar R, Banerjee N (2008) An
insecticidal GroEL protein with chitin binding activity from Xenorhabdus nematophila. J Biol
Chem 283:28287–28296
Kaur S (2000) Molecular approaches towards development of novel Bacillus thuringiensis
biopesticides. World J Microbiol Biotechnol 16:781–793
Khan AG (2005) Role of soil microbes in the rhizospheres of plants growing on trace metal
contaminated soils in phytoremediation. J Trace Elem Med Biol 18:355–364
Khandelwal P, Banerjee-Bhatnagar N (2003) Insecticidal activity associated with the outer
membrane vesicles of Xenorhabdus nematophilus. Appl Environ Microbiol 69:2032–2037
Khandelwal P, Choudhury D, Birah A, Reddy MK, Gupta GP, Banerjee N (2004) Insecticidal pilin
subunit from the insect pathogen Xenorhabdus nematophila. J Bacteriol 186:6465–6476
Klein MG (1990) Efficacy against soil-inhabiting insect pests. In: Gaugler R, Kaya HK (eds)
Entomopathogenic nematodes in biological control. CRC, Boca Raton, pp 195–231
Konig W, Faltin Y, Scheffer J, Schoffler H, Braun V (1987) Role of cell-bound hemolysin as a
pathogenicity factor for Serratia infections. Infect Immun 55:2554–2561
Kumari P, Kant S, Zaman S, Mahapatro GK, Banerjee N, Sarin NB (2014) A novel insecticidal
GroEL protein from Xenorhabdus nematophila confers insect resistance in tobacco. Trans-
genic Res 23:99–107
Lacey LA, Kaya HK (2007) Field manual of techniques in invertebrate pathology, 2nd edn.
Springer, Dorcrecht
Lacey LA, Frutos R, Kaya HK, Vail P (2001) Insect pathogens as biological control agents: do
they have a future? Biol Control 21:230–248. doi:10.1006/bcon.2001.0938
Lamelas A, Gosalbes MJ, Manzano-Marı́n A (2011) Serratia symbiotica from the aphid Cinara
cedri: A missing link from facultative to obligate insect endosymbiont. PLoS Genet 7:1–11
Landsberg MJ, Jones SA, Busby JN, Rothnagel R, Busby JN, Marshall SDG, Simpson RM, Lott
JS, Hankamer B, Hurst MRH (2011) 3D structure of the Yersinia entomophaga toxin complex
and implications for insecticidal activity. Proc Natl Acad Sci USA 108:20544–20549
Lee SC, Stoilova-McPhie S, Baxter L, Fülöp V, Henderson J, Rodger A, Roper DI, Scott DJ,
Smith CJ, Morgan JA (2007) Structural characterization of the insecticidal toxin Xpt A1
reveals a 1.15 MDa tetramer with a cage like structure. J Mol Biol 366:1558–1568
Lengyel K, Lang E, Fodor A, Szállás E, Schumann P, Stackebrandt E (2005) Description of four
novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus budapestensis
sp. nov., Xenorhabdus ehlersii sp. nov., Xenorhabdus innexi sp. nov. and Xenorhabdus
szentirmaii sp. nov. Syst Appl Microbiol 28:115–122
Li M, Wu G, Liu C, Chen Y, Qiu L, Pang Y (2009) Expression and activity of a probable toxin
from Photorhabdus luminescens. Mol Biol Rep 36:785–790. doi:10.1007/s11033-008-9246-z
Liu D, Burton S, Glancy T (2003) Insect resistance conferred by 283-kDa Photorhabdus
luminescens protein TcdA in Arabidopsis thaliana. Nat Biotechnol 21:1307–1313
Liu W, Ye W, Wang Z, Wang X, Tian S, Cao H, Lian J (2006) Photorhabdus luminescens toxin-
induced permeability change in Manduca sexta and Tenebrio molitor midgut brush border
membrane and in unilamellar phospholipid vesicle. Environ Microbiol 8:858–870
Marra MC, Piggott NE, Goodwin BK (2010) The anticipated value of SmartStax™ for US corn
growers. AgBio Forum 13:1–12
Mohan S, Sabir N (2005) Biosafety concerns on the use of Photorhabdus luminescens as bio-
pesticide: experimental evidence of mortality in egg parasitoid Trichogramma spp. Curr Sci
89:1268–1272
Moran NA (2002) Microbial minimalism: genome reduction in bacterial pathogens. Cell 108:
583–586
Morgan JA, Sergeant M, Ellis D, Ousley M, Jarrett P (2001) Sequence analysis of insecticidal
genes from Xenorhabdus nematophilus PMFI296. Appl Environ Microbiol 67:2062–2069
Naranjo SE (2005) Long-term assessment of the effects of transgenic Bt cotton on the function of
the natural enemy community. Environ Entomol 34:1211–1223
Neale MC (1997) Bio-pesticides – harmonisation of registration requirements within EU directive
91–414. An industry view. Bull Eur Mediterranean Plant Protect Organ 27:89–93
Nuñez-Valdez ME, Calder on MA, Aranda E, Hernández L, Ramı́rez-Gama RM, Lina L,
Rodrı́guez-Segura Z, Gutiérrez M, Villalobos FJ (2008) Identification of a putative Mexican
strain of Serratia entomophila pathogenic against root-damaging larvae of Scarabaeidae
(Coleoptera). Appl Environ Microbiol 74:802–810
Ochman H, Lawrence JG, Groisman EA (2000) Lateral gene transfer and the nature of bacterial
innovation. Nature 405:299–304
Ogier JC, Calteau A, Forst S, Blair HG, Roche D, Rouy Z, Suen G, Zumbihl R, Givaudan A,
Tailliez P, Médigue C, Gaudriault S (2010) Units of plasticity in bacterial genomes: new
insight from the comparative genomics of two bacteria interacting with invertebrates,
Photorhabdus and Xenorhabdus. BMC Genomics 11:568
Ohno S (1970) Evolution by gene duplication. Springer, New York, p 160
Parkhill J, Wren BW, Thomson NR (2001) Genome sequence of Yersinia pestis, the causative
agent of plague. Nature 413:523–527
Paul VJ, Frautschy S, Fenical W, Nealson KH (1981) Antibiotics in microbial ecology: isolation
and structure assignment of several new antibacterial compounds from the insect-symbiotic
bacteria Xenorhabdus spp. J Chem Ecol 7:589–597
Pechy-Tarr M, Bruck DJ, Maurhofer M, Fischer E, Vogne C, Henkels MD, Donahuer M,
Grunder J, Loper JE, Keel C (2008) Molecular analysis of a novel gene cluster encoding an
insect toxin in plant-associated strains of Pseudomonas fluorescens. Environ Microbiol 10:
2368–2386
Pereira EJ, Lang BA, Storer NP, Siegfried BD (2008) Selection for Cry1F resistance in the
European corn borer and cross-resistance to other Cry toxins. Entomol Exp Appl 126:115–121
Perlak F, Oppenhuizen M, Gustafson K, Voth R, Sivasupramaniam S, Heering D, Carey B, Ihrig
RA, Roberts JK (2001) Development and commercial use of Bollgard cotton in the USA-early
promises versus today’s reality. Plant J 27:489–502
Perry RD, Fetherston JD (1997) Yersinia pestis – etiologic agent of plague. Clin Microbiol Rev 14:
35–66
Poinar GO Jr (1979) Nematodes for the biological control of insects. CRC, Boca Raton, p 227
Poinar GO (1993) Origins and phylogenetic relationships of the entomophilic rhabditids,
Heterorhabditis and Steinernema. Fund Appl Nematol 16:333–338
Radake SG, Undirwade RS (1981) Seasonal abundance and insecticidal control of shoot and fruit
borer, Earias spp. on okra, Abelmoschus esculentus (L.). Indian J Entomol 43:283–287
Rae R, Riebesell M, Dinkelacker I (2008) Isolation of naturally associated bacteria of necromenic
Pristionchus nematodes and fitness consequences. J Exp Biol 211:1927–1936
Reineke A, Karlovsky P, Zebitz CPW (1999) Amplified fragment length polymorphism
analysis of different geographic populations of the gypsy moth, Lymantria dispar (Lepidop-
tera: Lymantriidae). Bull Entomol Res 89:79–88
BCC Research (2010) Biopesticides: the global market-BCC Research Report, CHM029C,
February 2010
Ribeiro C, Vignes M, Brehelin M (2003) Xenorhabdus nematophila (Enterobacteriaceae) secretes
a cation selective calcium-independent porin which causes vacuolation of the rough endo-
plasmic reticulum and cell lysis. J Biol Chem 278:3030–3039
Ruffner B, Péchy-Tarr M, Ryffel F, Hoegger P, Obrist C, Rindlisbacher A, Keel C, Maurhofer M
(2013) Oral insecticidal activity of plant-associated Pseudomonads. Environ Microbiol 15:
751–763. doi:10.1111/j.1462-2920.2012.02884.x
Ruiu L, Satta A, Floris I (2013) Emerging entomopathogenic bacteria for insect pest management.
Bull Insectol 66:181–186
Sanahuja G, Banakar R, Twyman RM, Capell T, Christou P (2011) Bacillus thuringiensis: a
century of research, development and commercial applications. Plant Biotechnol J 9:283–300
Sanchis V (2011) From microbial sprays to insect-resistant transgenic plants: history of the
biospesticide Bacillus thuringiensis. A review. Agron Sustain Dev 31:217–231. doi:10.1051/
agro/2010027
Saour G (2014) Sterile insect technique and F1 sterility in the European grapevine moth,
Lobesia botrana. J Insect Sci 14:8
Sayyed AH, Moores G, Crickmore N, Wright DJ (2008) Cross-resistance between a Bacillus
thuringiensis Cry toxin and non-Bt insecticides in the diamondback moth. Pest Manag Sci 64:
813–819
Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998)
Sergeant M, Jarrett P, Ousely M, Morgan AW (2003) Interactions of insecticidal toxin gene
products from Xenorhabdus nematophila PMF1296. Appl Environ Microbiol 69:3344–3349
Sergeant M, Baxter L, Jarret P (2006) Identification, typing, and insecticidal activity of
xenorhabdus isolates from entomopathogenic nematodes in United Kingdom soil and
characterization of the xpt toxin loci. Appl Environ Microbiol 72:5895–5907
Shahid AA, Rao AQ, Bakhsh A, Husnain T (2012) Entomopathogenic fungi as biological
controllers: new insights into their virulence and pathogenicity. Arch Biol Sci Belgrade 64:
21–42
Sheets JJ, Hey TD, Fencil KJ, BurtonS NW, Lang AE, Benz R, Aktories K (2011) Insecticidal
toxin complex proteins from Xenorhabdus nematophilus: structure and pore formation. J Biol
Chem 286:22742–22749
Sicard M, Le Brun N, Pages S, Godelle B, Boemare N, Moulia C (2003) Effect of native
Xenorhabdus on the fitness of their Steinernema hosts: contrasting types of interactions.
Parasitol Res 91:520–524
Sicard M, Ferdy JB, Pages S, LeBrun N, Godelle B, Boemare N, Moulia C (2004) When mutualists
are pathogens: an experimental study of the symbioses between Steinernema (entomopatho-
genic nematodes) and Xenorhabdus (bacteria). J Evol Biol 17:985–993
Sikorowski PP, Lawrence AM, Inglis GD (2001) Effects of Serratia marcescens on rearing of the
tobacco budworm (Lepidoptera: Noctuidae). Am Entomol 47:51–60
Silva CP, Waterfield NR, Daborn PJ, Dean P, Chilver T, Au CP, Sharma S, Potter U, Reynolds SE,
ffrench-Constant RH (2002) Bacterial infection of a model insect: Photorhabdus luminescens
and Manduca sexta. Cell Microbiol 4:329–339
Somvanshi VS, Lang E, Ganguly S, Swiderski J, Saxena AK, Stackebrandt E (2006) A novel
species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus indica sp. nov, symbioti-
cally associated with entomopathogenic nematode Steinernema thermophilum. Syst Appl
Spinner JL, Jarrett CO, LaRock DL (2012) Yersinia pestis insecticidal-like toxin complex
(Tc) family proteins: characterization of expression, subcellular localization, and potential
role in infection of the flea vector. BMC Microbiol 12:1–14
Srinivasan A, Giri A, Gupta V (2006) Structural and functional diversities in Lepidopteran serine
proteases. Cell Mol Biol Lett 11:132–154
Steinhaus EA (1941) A study of bacteria associated with thirty species of insects. J Bacteriol
42:757–790
Steinhaus EA (1951) Possible use of Bacillus thuringiensis Berliner as an aid in the biological
control of the alfalfa caterpillar. Hilgardia 20:350–381
Sulakvelidze A (2000) Yersinia other than Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis,
the ignored species. Microbes Infect 2:497–513
Swihart KG, Welch RA (1990) Cytotoxic activity of the Proteus hemolysin HpmA. Infect Immun
58:1861–1869
Tailliez P, Pagès S, Ginibre N, Boemare N (2006) New insight into diversity in the genus
Xenorhabdus, including the description of ten novel species. Int J Syst Evol Microbiol 56:
2805–2818
Talekar NS, Shelton AM (1993) Biology, ecology, and management of the Diamondback moth.
Vallet-Gely I, Opota O, Boniface A, Novikov A, Lemaitre B (2010) A secondary metabolite acting
as a signalling molecule controls Pseudomonas entomophila virulence. Cell Microbiol 12:
1666–1679
Vermunt AMW, Koopmanschap AB, VLak JM, de Kort CAD (1997) Cloning and sequence
analysis of cDNA encoding a putative juvenile hormone esterase from the Colorado potato
beetle. Insect Biochem and Mol Biol 27:919–928
Vigneux F, Zumbihl R, Jubelin G, Ribeiro C, Poncet J, Baghdiguian S, Givaudan A, Brehélin M
(2007) The xaxAB genes encoding a new apoptotic toxin from the insect pathogen
Xenorhabdus nematophila are present in plant and human pathogens. J Biol Chem 282:
9571–9580
Vodovar N, Vinals M, Liehl P, Basset A, Degrouard J, Spellman P, Boccard F, Lemaitre B (2005)
Drosophila host defense after oral infection by an entomopathogenic Pseudomonas species.
Proc Natl Acad Sci USA 102:11414–11419
Vodovar N, Vallenet D, Cruveiller S, Rouy Z, Barbe V, Acosta C, Cattolico L, Jubin C, Lajus A,
Segurens B, Vacherie B, Wincker P, Weissenbach J, Lemaitre B, Médigue C, Boccard F (2006)
Complete genome sequence of the entomopathogenic and metabolically versatile soil bacte-
rium Pseudomonas entomophila. Nat Biotechnol 24:673–679
Wang JX, Li LY (1987) Entomogenous nematode research in China. Rev Nematol 10:483–489
Waterfield N, Dowling A, Sharma S (2001) Oral toxicity of Photorhabdus luminescens W14 toxin
complexes in Escherichia coli. Appl Environ Microbiol 67:5017–5024
Waterfield NR, Daborn PJ, ffrench-Constant RH (2002) Genomic islands in Photorhabdus. Trends
Waterfield NR, Daborn PJ, Dowling AJ, Yang G, Hares M, ffrench-Constant RH (2003) The
insecticidal toxin makes caterpillars floppy 2 (Mcf2) shows similarity to HrmA, an avirulence
protein from a plant pathogen. FEMS Microbiol Lett 229:265–270
Waterfield N, Hares M, Yang G, Dowling A, ffrench-Constant R (2005a) Potentiation and cellular
phenotypes of the insecticidal Toxin complexes of Photorhabdus bacteria. Cell Microbiol
7:373-382
Waterfield N, Kamita SG, Hammock BD, ffrench-Constant R (2005b) The Photorhabdus Pir
toxins are similar to a developmentally regulated insect protein but show no juvenile hormone
esterase activity. FEMS Microbiol Lett 245:47–52
Waterfield NR, Sanchez-Contreras M, Eleftherianos I, Dowling I, Yang G, Wilkinson P, Parkhill J,
Thomson N, Reynolds SE, Bode HB, Dorus S, ffrench-Constant R (2008) Rapid virulence
annotation (RVA): identification of virulence factors using a bacterial genome library and
multiple invertebrate hosts. Proc Natl Acad Sci USA 105:15967–15972
Waterfield NR, Ciche T, Clarke D (2009) Photorhabdus and a host of hosts. Annu Rev Microbiol
63:557–574
Whitehouse MEA, Wilson LJ, Fitt GP (2005) A comparison of arthropod communities in
transgenic Bt and conventional cotton in Australia. Environ Entomol 34:1224–1241
Wilkinson P, Waterfield NR, Crossman L, Corton C, Sanchez-Contreras M, Vlisidou l, Barron A,
Bignell A, Clark L, Ormond D, Mayho M, Bason N, Smith F, Simmonds M, Churcher C,
Harris D, Thompson NR, Quail M, Parkhill J, ffrench-Constant RH (2009) Comparative
genomics of the emerging human Pathogen Photorhabdus asymbiotica with the insect pathogen
Photorhabdus luminescens. BMC Genomics 10:302–324
Woese CR (1987) Bacterial evolution. Microbiol Rev 51(221):271
Wolters M, Boyle EC, Lardong K (2013) Cytotoxic necrotizing factor-Y boosts Yersinia effector
translocation by activating Rac. J Biol Chem 288:23543–23553
Wu K, Lu Y, Feng H, Jiang Y, Zhao J (2008) Suppression of Cotton Bollworm in multiple crops in

china in areas with Bt toxin-containing cotton. Science 321:1676–1678
Yang G, Dowling AJ, Gerike U, ffrench-Constant RH, Waterfield NR (2006) Photorhabdus
virulence cassettes confer injectable insecticidal activity against the wax moth. J Bacteriol
188:2254–2261
Young SD, Townsend RJ, Swaminathan J, O’Callaghan M (2010) Serratia entomophila-coated
seed to improve ryegrass establishment in the presence of grass grubs. N Z Plant Protect 63:
229–234
Chapter 6
The Management of Spodopteran Pests Using
Fungal Pathogens
Md. Aslam Khan and Wasim Ahmad
6.1 Introduction
An estimated one third of global agricultural production valued at several billion

dollars is destroyed annually by insect pests in field and storage (Mariapackiam and
Ignacimuthu 2008). As the world population increases, the need to keep insects
away from destroying food crops becomes even more urgent. Insects are central to
the performance of many ecosystem processes. The class insecta contains nearly
one million described species (May 2000) which comprise approximately 67 % of
the world described fauna and flora. However, it is in their role as herbivores that
conflicts arise with agricultural production due to direct consumption of cultivated
crops and indirect damage by plant virus transmission resulting in reduction of
potential yield. According to Oerke and Dehne (2004), insect pests are responsible
for an estimated 42 % of all losses in crop production.
The last century has been witness to both the keynote augmentation of the
chemical pesticides as well as its backlashes. Insecticides of synthetic origin have
been used to manage insect pests for more than 50 years (Charnley and Collins
2007). Misuse of chemical pesticides and their negative impacts on soil and water
quality, human health, wildlife, and the ecological balance within agroecosystems
are increasingly becoming a concern, underlining the need for development of
alternative eco-friendly pest control methods (Aktar et al. 2009). In view of
growing public demand for food with low or no chemical residues, frantic efforts
are being directed towards nonchemical and sustainable plant protection (Flexner
and Belnavis 2000).
M.A. Khan
Department of Biology, Faculty of Science, Jazan University, Jazan, Saudi Arabia
W. Ahmad (*)
Section of Nematology, Department of Zoology, Aligarh Muslim University, Aligarh 202002,
India

DOI 10.1007/978-3-319-14499-3_6
124 M.A. Khan and W. Ahmad
Biological control agents are considered suitable alternatives to the use of

chemical pesticides as these organisms are highly specific to host insects, besides
being safe to the environment and mankind. Though the bio-management is not as
rapid as the chemicals, it is practically more feasible and sustainable than chemical
cure (McClintock et al. 2000). Biological control is defined as the use of living
organisms to suppress the population density or impact of a specific pest organism,
making it less abundant or less damaging than it would otherwise be (Eilenberg
et al. 2001). Thus, the aim of biological control is to reduce pest population below
the economic threshold. Many biological control agents are being employed for the
abatement of pest and vector insects of veterinary and medical importance (Burges
1981; Tanada and Kaya 1993; Lacey and Kaya 2000). Natural enemies such as
predators, parasitic wasps, flies, and insect pathogens have long been studied for
exploitation in biological control and integrated pest management (IPM) strategies.
In recent years, biological control of crop pests with entomopathogenic bacteria,
viruses, fungi, and nematodes has been recognized as a valuable tool in pest
management (Bhattacharya et al. 2003; Rosell et al. 2008).
Microbial control includes all aspects of utilization of microorganisms or their
by-products for the control of pests. Microbial control agents are relatively host
specific and do not upset other biotic systems. They are safe to humans, vertebrates,
and beneficial organisms; do not cause environmental pollution; and are compatible
with most other control methods. They are ideal for both short- and long-term pest
suppression. Unlike chemical pesticides, they do not leave chemical residues on
crops, are easy and safe to dispose off, and do not contaminate water systems.
Compared to other microorganisms, entomopathogenic fungi have received consi-
derable attention as they are exceptionally virulent and function as lethal parasites
of insect pests. Fungal diseases are known to cause insect mortality naturally
(Roberts and Humber 1981; Vimladevi and Prasad 2001; Gupta 2003). Some
entomopathogenic fungi cause regular epizootics (Devi et al. 2003; Rios
et al. 2010), resulting in very high levels of mortality in host populations.
Entomopathogenic fungi are potential agents for pest control due to their host
specificity, broad host range, mode of action, ease of production, and application
(Dhaliwal and Koul 2007; Ignacimuthu 2008). This has promoted the evaluation of
the entomopathogenic fungi as biocontrol agents in many countries. This chapter
outlines the current state of knowledge of insect fungal pathogens, their use as
mycoinsecticides, with special reference to spodopteran pest.
6.2 Spodoptera spp.
Spodoptera spp. [Spodoptera litura (Fabricius), tobacco cutworm; S. littoralis

(Boisduval), Egyptian cotton leafworm; S. frugiperda (Smith), fall armyworm;
S. exigua (Hübner), beet armyworm; and S. exempta (Walker), African armyworm]
(Lepidoptera: Noctuidae) are major pests of subtropical and tropical agricultural
crops with extensive host range of economically important crops.
6 The Management of Spodopteran Pests Using Fungal Pathogens 125
Though described way back in the eighteenth century, the importance of S. litura
Fabricius, 1775, as pest was brought to light only in the beginning of the twentieth
century through the accounts of Willcock (1905), Lefroy (1908, 1909), and Fletcher
(1914) which marked the beginning of contemporary researches on this obnoxious
insect. S. litura is widely distributed throughout the world (Anand et al. 2009)
and has been reported to feed on 120 cultivated food plants all over the world
(http://www.cabi.org/isc/datasheet/44520), of which 40 are grown in India (Basu
1981; Muthukrishnan et al. 2005) including tobacco, cotton, groundnut, jute,
lucerne, maize, rice, soybeans, tea, cauliflower, cabbage, and castor (Matsuura
and Naito 1997; Sahayaraj and Paulraj 1998; Sharma and Bisht 2008). The moth
is dark, with wavy markings on the fore wings and white hind wings and margin
having a brown color. The moths have a flight range of 1.5 km during a period of 4 h
overnight, facilitating dispersion and oviposition on different host plants (Salama
and Shoukry 1972). They can accordingly fly quite long distances.
S. littoralis is similarly one of the most destructive agricultural lepidopteran
pests within its subtropical and tropical range. Larvae of S. littoralis attack numer-
ous economically important crops belonging to 44 different families including
legumes, crucifers, grasses, and deciduous fruit trees, all the year round (Abdel-
Megeed 1975). On cotton, the pest may cause considerable damage by feeding on
the leaves, fruiting points, flower buds, and, occasionally, also on bolls. In toma-
toes, larvae bore into the fruit which is thus rendered unsuitable for consumption.
The pest could be active 9 months of a year and complete a generation within
30 days (Gharib 1979).
S. frugiperda is well known as a voracious insect pest of multiple agricultural
crops in the Western Hemisphere (Clark et al. 2007; Murua et al. 2009). It is one of
the most destructive insect pests attacking corn in the USA.
S. exigua was first discovered in North America around 1876 and reached
Florida in 1924. In warm locations such as Florida, all stages can be found
throughout the year, although development rate and overall abundance are reduced
during the winter months (Tingle and Mitchell 1977). The life cycle can be
completed in as few as 24 days, and six generations have been reared during
5 months of summer weather in Florida (Wilson 1934). The beet armyworm has
a wide host range, occurring as a serious pest of vegetable, field, and flower crops.
Weeds also are suitable for larval development. Larvae feed on both foliage and
fruit. In Florida it is regarded as a serious defoliator of flower crops and cotton,
though much of the injury is induced by insecticide use that interferes with natural
enemy activity. Young larvae feed gregariously and skeletonize foliage. As they
mature, larvae become solitary and eat large irregular holes in foliage. They also
burrow into the crown or center of the head on lettuce, or on the buds of cole crops.
As a leaf feeder, beet armyworm consumes much more cabbage tissue than the
diamondback moth, Plutella xylostella (Linnaeus), but is less damaging than the
cabbage looper, Trichoplusia ni (Hübner) (East et al. 1989). Tomato fruit is most
susceptible to injury, especially near fruit maturity, but beet armyworm is not
considered to be as threatening to tomato as is the corn earworm, Helicoverpa
zea (Boddie) (Zalom et al. 1986).
6.2.1 Biology
In S. litura, female lays 1,000–2,000 eggs in whitish-yellow egg masses of 100–300

on the lower leaf surface of the host plant (Miyahara et al. 1971). The masses are
covered by hairlike scales from the end of the insect’s abdomen. Fecundity is
adversely affected by high temperature and low humidity. The eggs hatch in
about 4 days in warm conditions, or up to 11–12 days in winter. The larvae pass
through six instars in 15–23 days at 25–26 C. The young larvae (first to third instar)
feed in groups, leaving the opposite epidermis of the leaf intact. Grown-up larva is
stout, cylindrical, and pale greenish brown with dark markings, some have trans-
verse and longitudinal gray and yellow bands, and others have rows of dark spots.
Later, the (fourth to sixth instar) larvae disperse and spend the day in the ground
under the host plant (Fig. 6.1), feeding at night and early in the morning. The pupal
period is spent in earthen cells in the soil and lasts about 11–13 days at 25 C.
Longevity of adults is about 4–10 days, being reduced by high temperature and low
humidity. Thus, the life cycle generally completes in about 5 weeks, while Saeedeh
et al. (2013) reported completion of life cycle between 29 and 35 days at different
Fig. 6.1 Different developmental stages of Spodoptera litura. (a) Egg mass. (b) Larva. (c) Pupae.
(d) Adult moth (photos courtesy: Dr. K. Sowjanya Sree, Amity University UP, India)
temperature and humidity. In the seasonal tropics, several generations develop

during the rainy season, while the dry season is survived in the pupal stage.
Biological parameters, larval and pupal development, and survival, longevity, and
fecundity of adults of S. litura are affected by the host plants (Ming et al. 2010).
6.3 Entomopathogenic Fungi
Entomopathogenic fungi, also referred as disease-causing fungi among insects,

contribute to the natural regulation of insect populations (Butt et al. 2001; Kleespies
et al. 2008). These fungi are among the first microorganisms to be used for the
biological control of insect pests. They are cosmopolitan organisms which have
been isolated from soils and infected insects from around the world. The natural
occurrence of these fungi is also documented by numerous researchers as summa-
rized in the excellent review by Zimmermann (2007a, b, 2008). More than 700 spe-
cies of fungi from around 90 genera are pathogenic to insects (Thackar 2002;
Wraight et al. 2007; Khachatourians and Sohail 2008; Hemasree 2013); however,
only few have been thoroughly investigated for their use against insect pests in
agriculture.
Modern exploration of entomopathogenic fungi began with the work of Russian
entomologist, Metschnikoff, who conducted first systematic experiments on the
control of injurious insects by infecting grubs of the grain beetle, Anisopliae
austriaca, with the green muscardine fungus, Metarhizium anisopliae Sorokin.
But the real breakthrough in the development of microbial control agents came
with the discovery and practical application of the milky disease bacteria, Bacillus
popilliae Dutky, for the control of Japanese beetle, Popilliae japonica Newman, in
the USA during the 1940s (Dhaliwal and Koul 2007).
The most widespread insect pathogenic fungal genera are found in the order
Hypocreales, phylum Ascomycota, viz., Metarhizium and Beauveria. M. anisopliae
and B. bassiana (Balsamo) Vuillemin, the white muscardine fungus, are the two
most recognized species (Vincent et al. 2007). They grow naturally throughout the
world and act as parasites of many arthropod species causing white and green
muscardine diseases due to the color of their spores (Vincent et al. 2007). There has
been overwhelming interest in exploring the potential for B. bassiana and
M. anisopliae.
Entomopathogenic fungi possess added advantage over other microbial control
agents as they are capable of attacking all developmental stages of insects including
pupal stages (Ferron 1978; Anand et al. 2009). Most importantly, these fungi are the
principal pathogens of sucking pests, since these hosts cannot ingest other patho-
gens like bacteria or viruses that infect through gut wall. None of the
entomopathogenic fungi currently in use or under consideration are invasively
pathogenic to humans (Kubicek and Druzhinina 2007). Certain ecological niches
lend themselves particularly well to the deployment of mycoinsecticides.
B. bassiana, M. anisopliae, and Lecanicillium (Verticillium) lecanii (Zimmermann)

are intensively studied as common natural enemies and important epizootics of
agricultural pests (Milner 1997; Roberts and St. Leger 2004; Wang et al. 2004;
Thomas and Read 2007; Li and Sheng 2007). Devi et al. (2003) reported epizootics
caused by Nomuraea rileyi Farlow (Samson) in south Indian fields during winter.
About 33.9 % of the mycoinsecticides are based on B. bassiana, followed by
M. anisopliae (33.9 %), Isaria fumosorosea (5.8 %), and B. brongniartii (4.1 %)
(Faria and Wraight 2007). B. bassiana is reported to limit the growth of plant
pathogenic fungi in vitro as well as reducing the diseases caused by soilborne plant
pathogens like Pythium, Rhizoctonia, and Fusarium (Ownley et al. 2010). Some
isolates of M. anisopliae are rhizosphere competent and can establish a symbiotic
relationship with plant roots (Hu and St. Leger 2002; St. Leger 2008).
6.3.1 Classification/Systematic Position
Entomopathogenic fungi are a very heterogeneous group of insect pathogens,

belonging to different systematic groups. Most entomopathogenic fungi are from
the fungal divisions Ascomycota and Zygomycota (Samson et al. 1988). The
Ascomycete fungi were previously divided into two groups, the Ascomycota and
the Deuteromycota. The Fungi Imperfecti of Deuteromycota was known for having
no sexual stage. But later, cultural and molecular studies have demonstrated that
some of these “imperfect fungi” were in fact anamorphs (asexual forms) of the
Ascomycota within the order Hypocreales (formerly called Hyphomycetes)
(Krasnoff et al. 1995; Fukatzu et al. 1997; Hodge 2003). Within Zygomycota, the
most entomopathogenic species are in the order Entomophthorales (Roy et al. 2006;
Hussain et al. 2012).
The genus Metarhizium presently includes several morphologically distinctive
species including M. anisopliae, M. album, M. flavoviride, M. cylindrosporae,
M. guizhouense, and M. pingshaense (Guo et al. 1986; Rath et al. 1995; Driver
et al. 2000) that cause diseases in different insect species. Despite recent interest in
the genetic diversity of many groups of entomopathogens, the genus Beauveria, in
contrast to other genera, has not received critical taxonomic review (Rehner
et al. 2011). Neelapu et al. (2009) studied molecular phylogeny of asexual
entomopathogenic fungi with special reference to B. bassiana and N. rileyi using
the sequences of partial regions of beta-tubulin and rRNA genes.
6.3.2 Dispersal/Transmission
Entomopathogenic fungi are widely distributed with both restricted and wide host
ranges having different biocontrol potentials against insect pest. Different insect
pathogenic mycofloras could be found in soil and in the overground environment.
Asexually produced fungal spores or conidia are generally responsible for infection
and are dispersed throughout the environment in which the insect hosts are present.
Entomopathogenic fungi are constantly present in populations of insect hosts, but
when density of the host population is normal, infections occur sporadically
(enzootic phase of insect diseases). However, during insects’ outbreak, fungi can
increase their numbers enough to spread in the environment and contribute to the
reduction of insect population (epizootic phase) (Fuxa and Tanada 1987).
Soil is a natural environment for entomopathogenic fungi. Many insects spend at
least part of its life in the soil; such natural behavior of insects related to their
biology, such as accumulation in the soil or leaf litter to wintering or pupation, is
conducive to fungal infections leading to natural reduction of many insect pests. It
has been shown experimentally that spores of the lepidopteran pathogen, N. rileyi,
adhere to leaves of plant seedlings as they emerge through the soil (Ignoffo
et al. 1977). Despite the fact that both B. bassiana and M. anisopliae are common
everywhere, B. bassiana seems to be very sensitive to disturbance and thus
restricted to natural habitats. Entomopathogenic fungi in the insect populations
may transmit horizontally (from infected insects on healthy individuals) or by
vectors. This second method plays an important role in the transmission of fungi
to new habitats (Fuxa and Tanada 1987).
6.3.3 Use of Fungi for Insect Biological Control
Entomopathogenic fungi can be employed for classical biological control, augmen-

tation, and conservation. Views by Jackson et al. (2000) and Butt et al. (2001)
provide further information. Classical biological control using fungal entomo-
pathogens can provide a successful and environment friendly avenue
for controlling arthropod pests, including the increasing numbers of invasive
nonnative species (Hajek and Delalibera 2010).
6.3.3.1 Classical Biological Control
Classical biological control is the intentional introduction of an exotic biological

control agent for permanent establishment and long-term pest control (Eilenberg
et al. 2001). In the case of microorganisms widely distributed in nature, the term
exotic means the use of a particular strain or biotype, which is not native to the area
where the pest is controlled. Introduced species to induce long-term effect has to
acclimate to the area under certain climatic conditions, multiply, and spread.
Entomophaga maimaiga was introduced from Japan into Eastern North America
in 1910–1911 to control the gypsy moth Lymantria dispar.
6.3.3.2 Augmentation
In many situations, natural enemies are present in indigenous pest populations, but
they are either too few or active too late to limit crop damage. In these cases, the
natural enemies can be augmented. There are two approaches to augmentation:
inoculation and inundation. In an inoculation approach, the fungus is applied, often
in small amounts, early in the season of the crop, with the expectation that it will
establish epizootics in pest populations and thereby maintaining the pest population
below the economic threshold. Inundative augmentation involves applying the
fungus, often in large amounts, for rapid short-term control with no expectation
for secondary infection (Eilenberg et al. 2001). In this way, the fungus is used in a
similar way to a chemical insecticide. The term “mycopesticide” or
“mycoinsecticide” have been used to describe this approach. Anamorphic
entomopathogenic fungi, such as B. bassiana and M. anisopliae, are usually
developed as inundative control agents which are applied in mass to a pest popu-
lation, and there is little expectation that they will persist and reproduce within the
biotic environment.
6.3.3.3 Conservation
Conservation biological control is modification of the environment or existing

practices to protect and enhance specific natural enemies or other organisms to
reduce the effect of pests (Fuxa 1998; Eilenberg et al. 2001). Fungal pathogens act
generally in a density-dependent fashion against their hosts and have relatively
slow kill. Therefore, epizootics of fungal pathogens on crop pests often occur too
late to be of economic value. One key advantage over chemical pesticides is lost if
an epizootic occurs too late or does not occur at all. Epizootics of nuclear poly-
hedrosis virus (NPV) are as common as fungal infections, but NPV requires high-
density populations for development of epizootics, whereas entomopathogenic
fungi can cause high level of infection in low-density as well as high-density
populations (Hajek et al. 1995). Mixed infections of entomopathogenic fungi and
viruses are also common in other pathogen-host systems. Specificity can be a
problem when there is a pest complex and no single pathogen can give control
(Powell and Jutsum 1993; Ravensberg 1994). In Columbia, there is a product
named Microbiol Completo, which is a mixture of B. bassiana, M. anisopliae,
N. rileyi, P. fumosoroseus, and the bacterium B. thuringiensis at 1 109 spores of
each pathogen per gram of product, for control of larvae and adults of Lepidoptera,
Coleoptera, Hemiptera, Diptera, and mites (Alves et al. 2003). An alternative is to
use two treatments sequentially. Early-season use of B. bassiana followed by
insecticides gave good control of beetle and caterpillar pests on crucifer crops
(Vandenberg et al. 1998).
6.3.4 Genetic Manipulation
The potential of entomopathogenic fungi often varies in different ecosystems and

among fungal species and strains. Natural strains of these fungi often lack sufficient
virulence or tolerance to adversity (Rangel et al. 2005). Therefore, genetic manip-
ulation is necessary to improve their efficacy and ecological fitness (Fang
et al. 2005; St. Leger and Wang 2009). In the process to improve the virulence of
entomopathogenic fungi, the extensive transcriptomic and genetic study of fungal
infection process revealed that a number of different genes were involved in the
pathogenicity (Freimoser et al. 2003; Wang et al. 2005; Cho et al. 2006a, b, 2007).
Therefore, highly virulent fungal genotypes against particular insect pest were
manipulated using molecular techniques. Development of recombinant DNA tech-
niques has made it possible to significantly improve the insecticidal efficacy of
fungi, bacteria, and viruses (Inceoglu et al. 2006; Wang and St. Leger 2007a, b, c).
These new types of biological insecticides offer a range of environment friendly
options for cost-effective control of insect pests (Federici et al. 2008). Integration of
insecticidal protein vip3Aa1 into B. bassiana enhances fungal virulence against
S. litura larvae (Yi et al. 2010). Fungal infection of aphids was accelerated by
overexpressing a silkworm chitinase or a hybrid chitinase in B. bassiana (Fang
et al. 2005; Fan et al. 2007). The realization of the economic potential of
mycoinsecticides would benefit from advances in biotechnology (Miranpuri and
Khachatourians 1995). Review by Raymond and Chengshu (2010) provides
comprehensive information for genetic engineering of fungal biocontrol agents.
6.3.5 General Biology of Entomopathogenic Fungi
The life cycle of most entomopathogenic fungi consists of two phases: a normal
mycelia growth phase mostly outside the host body and a yeastlike budding phase
mostly in the hemocoel of the host. When a spore adheres to the cuticle of insects, a
germ tube is generated and passed through the integument by mechanical and
enzymatic (e.g., chitinases, proteases, and lipases) process. When it reaches the
insect’s hemocoel, it produces blastospores which are the final pathogenic parts for
host infection (Vincent et al. 2007). Most, if not all, entomopathogenic fungi have
life cycles which synchronize with insect host stages and environmental conditions.
Complete developmental cycle of N. rileyi in S. litura lasted approximately 8–9
days (Srisukchayakul et al. 2005). Species, and sometimes isolates within a species,
can behave differently. For example, insect host range, infection levels, germina-
tion rates, and temperature optima can vary between species and isolates (Sierotzki
et al. 2000; Pell et al. 2001; Shaw et al. 2002). Members of the Hypocreales such as
Beauveria and Metarhizium spp. are opportunistic hemibiotrophs with a parasitic
phase in the live host and saprotrophic phase during postmortem growth on the
cadaver. Entomopathogenic fungi can invade their hosts directly through the
exoskeleton or cuticle; therefore, they can infect nonfeeding stages such as eggs and
pupae. Information on the biology and ecology of entomopathogenic fungi can be
obtained from Steinhaus (1949, 1964), Evans (1989), and Balazy (1993). Uribe and
Khachatourians (2008) have also described the life cycle of M. anisopliae under
liquid culture conditions.
6.3.6 Isolation of Entomopathogenic Fungi
6.3.6.1 From Insects
Entomopathogenic fungi have been isolated from infected insects and soil from
around the world. Several entomopathogenic fungal pathogens such as B. bassiana,
M. anisopliae, and Lecanicillium spp. with good potential for pest control had been
isolated from a variety of insects (Wraight et al. 2000; Faria and Wraight 2007).
Ramiro et al. (2013) isolated entomopathogenic fungus N. rileyi from dead
S. frugiperda larvae on corn. Satti and Gorashi (2013) isolated B. bassiana and
Paecilomyces sp. from infected dead beetle in Northern Sudan. Mudroncekova
et al. (2013) isolated entomopathogenic fungi from infected individuals of Ips
typographus. Before attempting to isolate a fungal pathogen, it is important to
keep the specimens fairly dry to avoid further deterioration by growth of sapro-
phytic fungi and bacteria. The following protocol is quite effective:
• Surface sterilize the insect by immersing it in alcohol or 5 % solution of Na–
HCl, HgCl2, or other suitable germicides for several minutes, and then rinse it in
three changes of sterile water.
• In a sterile dish, open the specimen and transfer a small portion of infected tissue
to a sterile culture plate. Saboraud’s dextrose agar with yeast extract produces
quick growth for entomogenous fungi, and the acid reaction (pH 5.6) retards
bacterial growth. The larva to be used for isolation of the fungus should not be
exposed to air once the sporulation is initiated. After sporulation of the fungus,
the Petri plate should be sealed with a parafilm and transferred to the refrigerator
to avoid any contaminants in isolation.
• The spore from the surface of the cadavers is streaked aseptically on the surface
of SMAY slopes and incubated as usual in the dark at 25 C. Mycelium and
spores can also be removed from a fresh specimen and placed directly on the
medium; however, this isolate should be compared with that obtained from the
infected tissues, since the chances of encountering a saprophytic fungus from the
surface of the specimen is much greater than from the internal tissue.
• The cultures can be placed in a moist incubator at 25 C and examined daily.
After growth and sporulation, fungi were identified based on the morphological
characters as per Humber (1997).
• After sporulation is complete, the slants are transferred to the refrigerator and
stored. For several fungi, sporulation continues for a week after transfer to the
refrigerator (Vimladevi and Prasad 2008).
6.3.6.2 From Soil
Soil is considered as excellent habitat for insect pathogenic fungi and other micro-
organisms since it is protected from UV radiation and buffered against extreme
biotic and abiotic influences (Keller and Zimmerman 1989). Fungal epizootics in
soil insect populations are also well documented (Samson et al. 1988; Keller and
Zimmerman 1989; Klingen and Haukeland 2006). Insect pathogenic fungi in the
genera Beauveria, Metarhizium, and Paecilomyces are all commonly found in the
soil (Domsch et al. 1980); David et al. (2003); Neuman and Shields (2004); Torasco
and poliseno (2005). Serigo et al. (2010) collected entomopathogenic fungi
B. bassiana and M. anisopliae from soil using bait method. Greater wax moth,
Galleria mellonella (L.) larvae were used as bait insects. The “Galleria bait
method” was first introduced by Zimmermann (1986) as a sensitive method to
detect a broad spectrum of insect pathogenic fungi in soil samples. Use of insect
bait is a very sensitive detection method, and entomopathogenic fungi can be
selectively isolated. Insect-associated fungi were detected in 55.5 % of the
425 soil samples collected from different field crops and orchards in China by
Sun and Liu (2008) using Galleria bait method. They also reported that diversity of
insect pathogenic fungi was greatest in field crop soil than in orchard. Sookar
et al. (2008) also reported three isolates of entomopathogenic fungi, B. bassiana,
M. anisopliae, and Paecilomyces fumosoroseus, from soil samples. Mudroncekova
et al. (2013) also isolated entomopathogenic fungi from soil samples. Further a
critical review by Hemasree (2013) on the natural occurrence of entomopathogenic
fungi in agricultural ecosystem provides complete information.
6.3.7 Mode of Action of Entomopathogenic Fungi
Unlike bacteria and viruses, which must be consumed, toxicity from entomo-
pathogenic fungi most often occurs from contact of the fungal conidia with the
host cuticle. Insect cuticle is composed of chitin fibrils embedded in a matrix of
proteins, lipids, pigments, and N-acylcatecholamines. Entomopathogenic fungi
secrete extracellular enzymes, proteases, chitinases, and lipases to degrade the
major constituents of the cuticle and allow hyphal penetration (Wang et al. 2005;
Cho et al. 2006b). The success of invasion is directly proportional to secretion of
exoenzymes (Khachatourians 1996). However, the ability of fungus to withstand
antifungal compounds such as short-chain fatty acids, in the insect cuticle,
is a prerequisite for successful invasion (Boucias and Pendland 1991). Numerous
light and electron microscope studies on the invasion of host cuticle by
entomopathogenic fungi are consistent with the involvement of both enzymes and
mechanical pressure. Besides exoenzymes, entomopathogenic fungi are reported to
produce toxin proteins (Roberts 1981; Gillespie et al. 2000; Strasser et al. 2000;
Freimoser et al. 2003). Toxin production is reported for B. bassiana, M. anisopliae,
and V. lecanii. However, B. bassiana is reported to produce low molecular weight
cyclic peptides and cyclosporins A and C with insecticidal properties (Roberts
1981; Vey et al. 2001). Some of the strains of B. bassiana are reported to produce
high molecular weight compounds with toxic activity against insect pest (Enrique
and Alain 2004). Maoye et al. (2014) mentioned great differences in chitinase
activities of Beauveria isolates.
The development of fungal infections in terrestrial insects is largely influenced
by environmental conditions. High humidity is vital for germination of fungal
spores and transmission of the pathogens from one insect to another. With most
entomopathogenic fungi, disease development involves the following steps:
Infection process starts with adhesion of conidia on the insect cuticle
(Srisukchayakul et al. 2005). Once the fungus breaks through the cuticle and
underlying epidermis, it may grow profusely in insect hemolymph. Fungus invade
host cuticle through the body wall and spiracles primarily and also through the
mouth parts (Hajek and St Leger 1994; Clarkson and Charnley 1996). The conidia
germinate on the insect cuticle by producing germ tubes which penetrate the body
wall. Growth of the fungus after it reaches the hemocoel is by budding which
produces hyphal bodies. These are transported throughout the hemocoel and give
rise to localized concentration of mycelia. A heavy growth of intertwining mycelia
develops in the hemocoel 1–2 days later. Insect death is probably the result of
starvation or physiological/biochemical disruption brought about by the fungus
(Feng et al. 1994). At the end of the infection cycle, mycelia emerge from the
cuticle and produce conidiophores (Srisukchayakul et al. 2005; Joseph et al. 2010).
Under suitable conditions, particularly high relative humidity, external sporulation
helps to spread the fungus and establish an epizootic.
6.3.8 Insect Immune Responses to Entomopathogenic Fungi
Insects have effective immune systems composed of both cellular and humoral
responses for fighting back against different kinds of microbial agents (Boman and
Hultmark 1987). When cuticular defense mechanism fails to overcome the invading
microorganisms, cellular defense regulated via interaction of different hemocytes is
initiated (Gupta et al. 2005). Hemolymph of insects is a medium for several
physiological processes like immune responses and intermediary metabolism.
When an invader enters the hemocoel of insects, hemocytes get engaged to remove
non-self-target by phagocytosis, nodule formation, encapsulation, synthesis of
antimicrobial peptides, and reactive metabolites (Beckage 2008). Meshrif
et al. (2011) reported a time-dependent decrease and increase in the phagocytosis
activity after injection of B. bassiana and N. rileyi, respectively, against S. littoralis.
Both fungi provoked a decrease of the total number of hemocytes at 48 h followed

by an increase at 72 h postinjection. Phenoloxidase enzyme is an important key
enzyme that triggered immune response in S. litura larvae against the invasion of
B. bassiana. Significant increase in PO level was observed at 4.0 106–
2.0 107 spores/ml after 24 h of infection in 3rd and 4th instar larvae of S. litura
(Gurmeet and Sanehdeep 2013). Study by Liu et al. (2014) gives an insight into
better understanding of the molecular mechanisms of innate immune processes in
Asian corn borer Ostrinia furnacalis larvae against B. bassiana. An excellent
review on entomopathogenic fungi versus insect cuticle by Almudena and Nemat
(2013) provides complete information on advances in broad-host-range
entomopathogenic fungi.
6.3.9 Compatibility of Entomopathogenic Fungi with Other

Crop Protection Techniques
The integration of biological and chemical control approaches is important for

successful IPM programs. Entomopathogenic fungi compatibility with other crop
protection techniques such as insecticides and commercial botanicals necessitates a
proper understanding of the development and reproduction of pathogen (Malo
1993). For successful biological control, the biology of the control agent and its
compatibility with other agents is important (Cuthbertson and Murchie 2007).
Fungal biological control agents and selective insecticide may act synergistically
increasing the efficiency of the control (Ambethgar 2009). By contrast, use of
incompatible insecticides may inhibit growth and reproduction of the pathogens.
Therefore, fungal genotypes compatible to particular pesticides can be identified
and manipulated. Approach is based on the assumption that, weakened by another
stressor, the insect will succumb more readily to mycosis. Beneficial effects were
observed in using the two fungi together compared to the separate application. In
China, combinations of B. bassiana and certain insecticides have been
recommended for application against crop and forest pests (Feng et al. 1994).
Synergy occurred between B. bassiana and imidacloprid against caterpillar
Spilarctia obliqua (Walker) (Purwar and Sachan 2006). Efficacy of B. bassiana
(Bb-L-2) and M. anisopliae (Ma-L-1) strains enhanced in combination with insecti-
cide, spinosad 45SC at 0.009 %, neem oil 5 %, and NSKE 5 % against S. litura
larvae (Udayababu et al. 2012).
Gopalkrishnan and Mohan (2000) tested seven insecticides and seven fungicides
which are commonly used for the control of pests and diseases of tomato for their
inhibitory effect on germination of conidia of N. rileyi at three (low, normal, and
high) concentrations in vitro. They concluded that monocrotophos, phosphomedon,
and dimethoate were safe to the mycopathogen at all the three concentrations.
Among the fungicides, captafol, zineb, chlorothalonil, fosetyl-Al, and ziram were
safe to the fungus at all the concentrations evaluated. Captan and sulfur on the other
hand, though allowed conidial germination at low concentrations but normal

concentrations, caused total inhibition at higher concentrations. No synergistic
effect was observed on pathogenicity in the combination treatments of
B. bassiana with L. muscarium (Naser et al. 2013). Asi et al. (2010) reported that
insecticides significantly inhibit mycelial growth and conidial germination of
M. anisopliae and P. fumosoroseus. Complete growth inhibition (100 %) of new
isolate IOF1 of N. rileyi was reported by Patil et al. (2013) in compatibility with
fungicides, carbendazim, and mancozeb at recommended doses, but insecticides
endosulfan and dichlorvos inhibit growth by 52.8 and 50.7 % respectively. Golshan
et al. (2013) reported highest adverse effect of fungicides on the germination of
B. bassiana isolates in the rice fields; however, they recorded highest germination
of fungal isolates in the herbicide treatments. Inhibitory effects of pesticides on
germination and mycelial growth of entomopathogenic fungi often vary among
fungal species and strains (Vanninen and Hokkanen 1988; Anderson et al. 1989).
Commercial botanicals and ethanol extracts of seeds and leaves also reduce the
mycelial growth of B. bassiana, I. fumosorosea, and L. lecanii (Sahayaraj
et al. 2011). Plant extracts, Annona squamosa and Polyalthia longifolia, showed
55.8 % growth inhibition of N. rileyi isolate (Patil et al. 2013).
6.4 Control Measures of Spodoptera spp.
6.4.1 Prophylactic Measures
Cultural practices like deep plowing of infested fields so as to expose the pupae to
the sun rays and insectivorous birds, destruction of weed and wild host plants,
burning of crop residues, growing of castor as ovipositional trap crop, and collec-
tion and destruction of egg masses and early instars had been in practice for a very
long time. Jayaraj (1978) added a new dimension to the above practices by
advocating the cultivation of less susceptible cotton varieties in Tamil Nadu. He
further suggested that rationing of cotton crop be stopped as it aggravates the pest
problem. Chari and Patel (1983) added that cultivation of summer crops like bhindi
(Abelmoschus esculentus) and lucerne (Medicago sativa) with irrigation be avoided
to keep the population of pest under check.
Light traps had been in use for a considerable long time as one of the important
tools of limiting the pest population. Pandey (1970) operated light traps and found
that early hours of the night provided better catches if ecological factors like wind,
rainfall, temperature, and humidity did not affect the pest adversely. Jayaraj (1978)
suggested that light traps may be operated between 7 p.m. and 11 p.m. for better
catches. Chari and Patel (1983) recommended blacklight traps for this pest. Tucker
(1983) studied the association between weather and light-trap catches of
Spodoptera and reported that high densities of flying moths, which may give rise
to caterpillar outbreaks early in the year, often occur where there is rather
infrequent early-season rainfall. Field trials on the population dynamics of S. litura

using light and pheromone traps indicated its activity throughout the year
(Nandihalli et al. 1989). Pheromone traps can be a viable alternative in integrated
management of tobacco cutworm infestation. It can help farmers to get higher
benefits by lowering costs and increasing yields than mere chemical control.
Adoption of sex pheromone trapping can effectively suppress adults, largely
decrease larvae or egg mass density, and damage rate of S. litura (Yang et al. 2009).
6.4.2 Chemical Insecticidal Control
Management of obnoxious lepidopteran pests relies mainly on the use of chemical

insecticides including carbamates, pyrethroids, and organophosphates (Liburd
et al. 2000). Chemical control of Spodoptera has been extensively reported on
various crops in India. Two common insecticides, DDT and BHC, which were
regarded as effective checks for all pest problems in the early parts of fifties and
sixties in India, were used against S. litura (Kurup and Joshi 1959). Later,
researchers recommended the use of strong insecticides like endrin and endosulfan
either singly or in combination with other insecticides for the control of S. litura
(Jotwani et al. 1961; Sarup and Singh 1974). Besides the chlorinated insecticides,
some very promising organophosphatic insecticides were also recommended for the
control of S. litura. Malathion, dimethoate, etc. were the common organo-
phosphatic insecticides recommended by various workers (Sarup 1970; Saad
et al. 1975). Yet another landmark in the insecticidal world was made by the
introduction of carbamates. Carbaryl, a potent carbamate insecticide, was
recommended for the control of S. litura (Yathom and Rivnay 1960; Sarup 1970).
Hazards and harmful effects of insecticides as chemical control especially the wide
application of conventional insecticides necessitate the new chemistry insecticides
which are more effective, safer for humans, and much less toxic to our ecosystem
(Korrat et al. 2012). Pyrethroids were least effective due to their high LC50 values
as compared to new chemistry insecticides (Bhatti et al 2013).
Resistance to insecticides is a major problem associated with the chemical
control of insect pests, which is characterized by rapid evolution under strong
selection of gene(s) that confers survival to insecticides (Ahmad et al. 2008). The
pests have developed high resistance against a wide variety of insecticides includ-
ing organophosphate, carbamate, and pyrethroids, resulting in their sporadic out-
breaks and failure of crops (Armes et al. 1997; Kranthi et al. 2001; Ahmad
et al. 2007a, b, 2008; Saleem et al. 2008). The management of the pest has therefore
become increasingly difficult all over the world. Shankarganesh et al. (2012)
observed resistance against conventional as well as new chemistry insecticides
such as quinalphos, monocrotophos, lindane, endosulfan, benzene hexachloride,
avermectins, spinosad, fipronil, indoxacarb, and chitin synthesis inhibitors. Tong
et al. (2013) reported field resistance of S. litura to carbamates (thiodicarb or
methomyl) was significantly higher than that of organophosphates and pyrethroids.
6.4.3 Plant Products/Biopesticides
Replacement of synthetic insecticides by bio-rational insecticide is universally

acceptable and is a practicable approach worldwide. Throughout history, plant
products have been successfully exploited as insecticides. Recent plant protection
research, particularly of the last decade, revealed the importance of plant products
that disrupt normal insect growth and development. Plants are rich sources of
natural substances that can be utilized in the development of environmentally
safe methods for insect control (Sadek 2003). Plants contain secondary metabolites
that are deleterious to insect pests in diverse ways; through acute toxicity, enzyme
inhibition and interference with the consumption and/or utilization of food
(Wheeler and Isman 2001) and the biomolecules present in phytopesticides act as
a feeding deterrent, ovicide, oviposition deterrent, and growth inhibitor against field
insect pests (Baskar et al. 2011; Baskar and Ignacimuthu 2012a, b; Arivoli and
Tennyson 2013). Several plant products have been screened and tested against
S. litura and some promising plants have been reported by Arivoli and Samuel
(2012, 2013). However, screening of plant extracts against this pest is still conti-
nuing throughout the world to find eco-friendly biopesticides.
6.4.4 Biological Control
Biological control represents an important strategy to reestablish the biodiversity of

agroecosystems, especially with the introduction of entomophagous organisms with
classic innoculative techniques or the increase of natural populations of predators,
parasitoids, and pathogens (Silva 2000).
6.4.4.1 Predators and Parasitoids
A number of predators [Andrallus spinidens and Harpactos costalis (Hemiptera:

Pentatomidae), Agrypnus fuscipes and Broscus punctatus (Coleoptera: Elateridae)
(Chari and patel 1983), and generalist predator Podisus nigrispinus (Heteroptera:
Pentatomidae)], widespread in the Neotropical region (Thomas 1992), have been
reported to feed on Spodopteran larvae (Batalha et al. 1997; Zanuncio et al. 1998;
2008).
Parasitoids [Apanteles flavipes, A. ruficrus, A. vitripennis, Chelonus heliope,
C. formosanus, Microphilis sp. (Hymenoptera: Braconidae); Blepharella lateralis,
Peribaea orbata, Strobliomyia egyptia (Diptera: Tachinidae)] limit the population
of Spodopteran pest (Chari and patel 1983). Sertkaya et al. (2004) reported braco-
nid larval parasitoids Microplitis rufiventris (Kokujev), M. tuberculifer (Wesmael),
Meteorus ictericus (Nees), Chelonus obscuratus (Herrich-Schaffer) (an egg-larval
parasitoid), and Apanteles ruficrus (Halliday); the ichneumonid larval parasitoids
Hyposoter didymator (Thunberg) and Sinophorus xanthostomus (Gravenhorst); and

the egg parasitoid Trichogramma evanescens (Westwood) as natural enemies
attacking S. exigua in Turkey.
6.4.4.2 Bacillus thuringiensis
Owing to its insecticidal activity, B. thuringiensis acquired tremendous significance

in agriculture. Many lepidopteran insect pests can effectively be controlled by
B. thuringiensis (Nethravathi et al. 2010). Literature revealed that thousands of
toxicogenic strains of B. thuringiensis exist (Lereclus et al. 1993). The efficacy of
biopesticides is dependent on the dose of the active ingredient (Prabagaran
et al. 2003; Knowles 2005). By restricting the amount of damage caused by
lepidopteran pests to the infested crop, B. thuringiensis-based biopesticides have
contributed to yield increases in different crops like rice (Kandibane et al. 2010),
cauliflower (Justin et al. 2003), and corn (Tamez-Guerra et al. 1998). Combination
of insecticides and B. thuringiensis had also been tried to limit the population of
lepidopteran pests (Khan et al. 2010).
6.4.4.3 Polyhedrosis Virus
Insect viruses, in particular nuclear polyhedrosis viruses (NPVs), however, seemed

useful biological control agents because of their virulence and host specificity
(Payne 1986; Bhutia et al. 2012). NPV of S. litura (spltNPV) has emerged as an
alternative to chemical insecticides for the management of S. litura (Ramakrishna
and Tiwari 1969). Several highly virulent NPVs have been isolated from S. exigua
larvae (Vlak and Groner 1980; Vlak et al. 1982; Gelernter and Federici 1986).
However, NPVs have both advantages as well as disadvantages. The narrow host
range of NPVs generally restricts their effectiveness against the complex of insect
pests in fields. The slow speed of their action against target insect is another
disadvantage of NPVs (Muralibaskaran et al. 1997). Only the in vivo production
of the baculoviruses has so far been economically viable due to the high costs
involved in the in vitro production systems (Shieh 1989; Kumar and Singh 2009).
6.4.4.4 Entomopathogenic Nematodes
Entomopathogenic nematodes (Rhabditida: Steinernematidae and Hetero-

rhabditidae) are insect pathogens with great potential for biological control (Kaya
and Gaugler 1993; Kaya 2006). Kaya (1985) reported that all larval instars of
S. exigua were susceptible to Steinernema feltiae (S. carpocapsae); however,
neonate larvae were significantly less susceptible to nematode infection than 3-
and 8-day-old larvae. The nematodes usually kill the host insects within 1–3 days
(Kaya 1985; Gothama et al. 1995). Entomopathogenic nematode, Heterorhabditis
indica, has been extensively studied for use in the control of various lepidopteran
pests damaging glasshouse and nursery crops (Gouge and Hague 1993; Grewal and
Richardson 1993; Elanchezhyan 2006; Divyaa et al. 2010).
6.5 Entomopathogenic Fungi
Insect pathogenic fungi naturally restrict the buildup of insect pests without any
interference. This part of the “law of natural balance” is being inadvertently
destroyed by the indiscriminate use of chemical pesticides. This has led to the
notion that all forms of pest control should be integrated and environmentally
acceptable to the agricultural ecosystem. Entomogenous fungi are potentially the
most versatile biological control agents, due to their wide host range that often
results in natural epizootics. Mycopathogens infect by direct penetration of the
cuticle (Wraight et al. 1998; 2000; Srisukchayakul et al. 2005) and are, therefore,
the principle pathogens among sucking insects which cannot ingest other patho-
gens. Fungi often cause spectacular epizootics with large number of pathogenic
insects showing visible fungal outgrowth (Hall 1982). Fungal biological control
agents have demonstrated efficacy against a wide range of insect pests including
Spodoptera spp. (Purwar and Sachan 2005; Lin et al. 2007; Amer et al. 2008).
M. anisopliae is known to attack over 200 species of insects belonging to orders
Lepidoptera, Coleoptera, Orthoptera, Homoptera, and Dermoptera (Moore
et al. 1996). However, V. lecanii popularly called the “white holo” is known to
cause mycosis in a number of insects belonging to the insect orders Lepidoptera,
Coleoptera, and Homoptera. It is certain that entomogenous fungi will continue to
increase their share very rapidly in the IPM. There is a general feeling that the
development and spread of biological control will empower the resource of poor
farmers to manage their pest problems in an eco-friendly way.
6.5.1 Entomopathogenic Fungus Beauveria
B. bassiana, the most common and ubiquitous fungal entomopathogen, is known to

be highly potent for the control of insects belonging to various orders. Wraight
et al. (2010) reported that all lepidopteran species of vegetable crop pests, viz., fall
armyworm (S. frugiperda), beet armyworm (S. exigua), diamondback moth
(Plutella xylostella), European corn borer (Ostrinia nubilalis), corn earworm
(Helicoverpa zea), black cutworm (Agrotis ipsilon), cabbage worm (Pieris
rapae), and cabbage looper (Trichoplusia ni), were susceptible to B. bassiana
isolates. However, S. exigua and H. zea were most susceptible to fungal infection
and S. frugiperda was least susceptible. Fungus negatively effects the biology of
S. litura. Kaur et al. (2011) observed significantly high larval mortality, reduced
reproductive potential and adult life span, and a decrease in egg hatching and larval
period along with pupal and adult deformities at sublethal doses of B. bassiana in
S. litura. Karthikeyan and Selvanarayanan (2011) reported 86.67, 86.67, and
73.33 % larval mortality of S. litura, H. armigera, and E. vittella, respectively,
caused by B. bassiana. Yet in another study, Malarvannan et al. (2010) noticed
adverse effects of B. bassiana on S. litura pupae, formed from treated larvae along
with adult malformation. Alice and Nadarajan (2003) reported natural occurrence
of B. bassiana on rice leaf folder Cnaphalocrocis medinalis Guenee, while red
hairy caterpillar Amsacta albistriga Walker was recorded infected with B. bassiana
in groundnut crop (Veena et al. 2006). Virulence test among five species of
entomogenic fungi, B. bassiana, B. brongniartii, M. anisopliae, N. rileyi, and
P. fumosoroseus, showed that B. brongniartii and N. rileyi had evident pathogenic
effects against 2nd instars of S. litura. The virulence of B. brongniartii and N. rileyi
to the 3rd instars was lower than that of 2nd instars (Lin et al. 2007).
The potential of entomopathogenic fungi often varies in different ecosystems
and among fungal species and strains. Larvicidal and growth inhibitory activities of
ten different isolates of B. bassiana from Pulney hills of Western Ghats of Tamil
Nadu, India, were evaluated against third instar larvae of S. litura. B. bassiana
isolate (Bb10) showed maximum larvicidal activity of 68.06 %, minimum pupal
weight of 183 mg, low number of adult emergence (22.91 %), and 100 % abnormal-
ities at 1 108 spore/ml concentration (Baskar et al. 2012). They also noticed dose-
dependent activities in all the tested isolates. Godonou et al. (2009) screened
indigenous isolates of B. bassiana for virulence against P. xylostella larvae and
reported 94 % mortality caused by B. bassiana isolate Bba5653. They further
reported approximately threefold higher yield compared to plots treated with the
insecticide, bifenthrin, or in untreated plots. Pathogenicity of the isolates was
greater when pathogens were obtained directly from host insect. B. bassiana
isolate Bb42 obtained from S. frugiperda larvae showed the highest virulence,
(96.6 % mortality) against second instar larvae of the same insect (Garcia
et al. 2011). In contrary to above results, Hung et al. (1993) reported that insect
mycopathogen B. bassiana and Candida albicans were rapidly phagocytized by
circulating hemocytes in S. exigua, resulting in the protection of challenged larva.
Bioassay of Beauveria isolates, under the concentrated standard spray of
1,000 conidia/mm2 against economically important rice pest Nilaparvata lugens,
resulted in 17.2–79.1 % adult mortality, 10 days after inoculation (Maoye
et al. 2014).
6.5.2 Entomopathogenic Fungus Metarhizium
Metarhizium, commonly known as green muscardine fungus, contains a number of

morphologically distinct species, M. anisopliae, M. album, M. cylindrosporae,
M. flavoviride, M. guizhouense, and M. pingshaense (Driver et al. 2000), which
germinate at low temperature (Meyling and Eilenberg 2007). Krutmuang
et al. (2008) reported M. anisopliae isolate BCC4849 to be highly effective against
third instar larvae of S. litura at 6 108 spores/ml concentration. However,
M. anisopliae isolate Ma002 showed 87.5 and 81.25 % larval mortality of S. exigua
under laboratory and semi-field conditions (Freed et al. 2012b). Udayababu
et al. (2012) estimated LC50 value (16.52 105 conidia/ml) of M. anisopliae
against third instar larvae of S. litura. Moraga et al. (2006) reported significant
mortality of S. littoralis larvae by crude protein extracts of M. anisopliae and
B. bassiana isolates. Quesada-Moraga et al. (2006) also successfully used the
crude protein extracts of M. anisopliae for the control of S. litura. Yet in another
study, Contreras et al. (2014) evaluated liquid formulation of M. anisopliae on
different populations of tomato borer T. absoluta and reported the potential of
M. anisopliae to control pupae of the lepidopteran borer at the recommended
rate, in IPM programs. Moreover, a notably lower dose was also sufficiently
effective to control the tomato borer populations. Entomopathogenic fungi secrete
extracellular enzymes to degrade the major constituents of the cuticle (Wang
et al. 2005; Cho et al. 2006b), and the success of invasion is directly proportional
to secretion of exoenzymes (Khachatourians 1996). Petlamul and Prasertsan (2012)
reported that in comparison with B. bassiana, M. anisopliae possessed the highest
enzyme activities against S. litura. M. anisopliae strain M6 and M8 possessed the
highest protease activity (145.00 mU/ml) and highest chitinase activity (20.00 mU/
ml) respectively during 96–144 h cultivation. Amer et al. (2008) reported higher
mortality among S. littoralis larvae treated with M. anisopliae isolates compared to
B. bassiana isolates. M. anisopliae formulations are also highly specific against
locusts and grasshoppers. As a result of M. anisopliae infection, physiological and
biochemical changes in the locusts are reported. Shereen et al. (2012) noticed a
decline in total insect proteins, carbohydrates, and lipid contents. The highest
physiological phenomena like immune responses and intermediary metabolisms
12 h postinjection by spores of M. anisopliae and B. bassiana on fifth larval instars
of S. littoralis (Mirhaghparast et al. 2013) as well as high pathogenicity of
M. anisopliae against European spruce bark beetle Ips typographus (Mudroncekova
et al. 2013) have been reported.
Since fungi infect by direct penetration of the insect cuticle, they are capable of
infecting even nonfeeding stages, eggs (Ujian and Shahzad 2007; Anand and
Tiwary 2009), and pupae of insects (Nguyen et al. 2007; Anand et al. 2009). Asi
et al. (2012) tested potential of strains of M. anisopliae along with B. bassiana,
I. fumosorosea, and L. Lecanii against eggs of S. litura and found significant
variation (37.50–78.00 %) in hatching of fungal-treated eggs. Entomopathogenic
fungi not only affect egg hatching but also infect larvae of S. litura after hatching.
Rajesh et al. (2009) reported the biocontrol potential of M. anisopliae,
L. muscarium, and C. cardinalis against pupae of polyphagous pest S. litura.
Fungi can also target sucking insects such as aphids, thrips, whiteflies, and mos-
quitoes. Jandricic et al. (2014) reported high virulence of entomopathogenic fungi
Metarhizium and Beauveria isolates against nymphal stage of aphids Myzus
persicae and Aphis gossypii. Nguya and Sunday (2013) reviewed the potential of
entomopathogenic fungi for the control of insect vector, tsetse flies.
Susceptibility of the insect to entomopathogenic fungi decreases with the
advancement in age of larvae (Asi et al. 2013); however, the larval mortality
increases with increase in conidial concentrations and time elapsed after treatment.
Pandey and Hasan (2009) also noticed that susceptibility of S. litura larvae against
M. anisopliae decreases with increase in age of larvae, in terms of both LC50 and
LT50.
6.5.3 Entomopathogenic Fungus Nomuraea
It is well known that N. rileyi induces extensive epizootics among caterpillar and it
has potential to be a microbial control agent for lepidopteran pests (Li-chang and
Roger 2004). Ingle et al. (2004) observed severe infections of N. rileyi in S. litura
and H. armigera on green gram along with lower incidence of the pathogen on
Bihar hairy caterpillar Spilarctia obliqua (Walker) in soybean. Vimaladevi and
Prasad (1997) reported epizootics of N. rileyi on S. litura and H. armigera in kharif
groundnut when relative humidity and temperature ranged between 70–92 % and
21–27 C. Manjula et al. (2003) in a survey for incidence of N. rileyi on S. litura and
H. armigera on tomato, cotton, black gram, red gram, and groundnut recorded
100 % mycosed larvae of S. litura and H. armigera on cotton and groundnut.
Sreedhar and Devaprasad (1995) reported higher percent of mycosis caused by
N. rileyi in S. litura larval population, when groundnut was used as food plant than
chillies, black gram, and tomato. Vimaladevi et al. (1996) reported epizootics of
N. rileyi on lepidopteran pests of oil seed crops. They observed more infected larvae
of S. litura in castor followed by groundnut and pigeon pea. Patil et al. (2013)
isolated N. rileyi from the cadavers of S. litura collected from groundnut field and
observed that early instars were highly susceptible with a mortality of 70.17 %,
which decreased significantly as the age of the larvae advanced. Lethal time for 1st
to 5th instars of S. litura larvae was 130.71, 137.77, 148.04, 235.65, and 263.10 h,
respectively. During rainy and summer seasons, Rachappa et al. (2007) recorded
ten entomopathogenic fungi on lepidopteran caterpillars in different crops.
Vimaladevi (1994) tested efficacy of N. rileyi against S. litura in a lab, net house,
and field and reported 2 1011 conidia/l of spray solution brings about effective
control of late second to early third instar S. litura larvae on castor crop. In the field,
larval mortality of 52–60 % was observed at 12 days after spraying with N. rileyi
conidia. The highest cumulative mortality, 88–97 %, was observed by 19 days.
Kulkarni and Lingappa (2002) conducted field experiments from 1996 to 1998 to
evaluate the bio-efficacy of N. rileyi on defoliator S. litura in soybean and reported
that pathogen achieved significantly higher reduction of Spodopteran larvae at
higher concentration (1.2 1012 conidia/l) at 14 days.
S. frugiperda has been reported to be susceptible to more than 20 species of
entomopathogenic fungi (Sanchez-Pena 2000); one of these is N. rileyi (Lezama
et al. 2001). Rios et al. (2010) reported natural epizootic of the entomopathogenic
fungus, N. rileyi infecting S. frugiperda. In laboratory bioassays, Domenico
et al. (2009) observed N. rileyi killing 80 % of S. frugiperda larvae.
Entomopathogenic fungal isolates from different geographical locations and
different hosts vary in their virulence and specificity (Tigano et al. 1995).
Vimaladevi et al. (2003) studied eleven geographical isolates of N. rileyi and
reported best traits to be the Karimnagar isolate of S. litura origin. Ramiro
et al. (2013) recorded the occurrence of native entomopathogens in S. frugiperda
larvae, collected from 22 localities of central Mexico, during 2009; N. rileyi was
recovered from 38 larvae, whereas unidentified microsporidia was found infecting
19 larvae.
6.5.4 Entomopathogenic Fungus Isaria
The entomopathogenic fungi I. farinosa and I. fumosorosea were known as

P. farinosus and P. fumosoroseus, for more than 30 years. Both fungi have
worldwide distribution and a relatively wide host range. While I. farinosa currently
is of minor importance in research and as biocontrol agent, I. fumosorosea is
regarded as a species complex, and various strains are successfully used for
biocontrol of several economically important insect pests (Zimmermann 2008).
Freed et al. (2012a) reported insecticidal and antifeedant activities of crude proteins
produced by I. fumosorosea against diamond back moth, P. xylostella. A significant
level of increase in the antifeedant index was recorded with the increase of
concentrations and time duration. Zemek et al. (2012) evaluated efficacy of two
strains of I. fumosorosea, Apopka 97 (an active ingredient of commercial bio-
pesticide, Biobest) and CCM 8367 (isolated from Cameraria ohridella), against
larvae and pupae of S. littoralis using standard dip test. Obtained results revealed
higher virulence of CCM 8367 to the last instar larvae of S. littoralis (93.1 %
mortality) compared to Apopka 97 (65.5 % mortality) on the 7th day. More obvious
difference was found in pupae, where mortality of CCM 8367-treated pupae was
80.0 %, while mortality in Apopka 97-treated pupae reached only 3.3 % on the 8th
day after the treatment. Hussein et al. (2013) also reported that I. fumosorosea strain
CCM 8367 has strong insecticidal effects against S. littoralis and has the potential
to be implemented as a novel biocontrol agent. Asi et al. (2012) tested various
strains of I. fumosorosea, B. bassiana, M. anisopliae, and L. lecanii against third
instar larvae of S. litura, using larval immersion method, and recorded more
profound effects of I. fumosorosea and B. bassiana. Strains with high virulence
potential also showed greater mycosis and sporulation on cadavers.
Three entomopathogenic fungi, I. fumosorosea, B. bassiana, and N. rileyi, were
compared against H. armigera in laboratory bioassays employing topical versus per
os inoculation techniques. N. rileyi outperformed both I. fumosorosea and
B. bassiana, causing a mean 87 % mortality. However, no difference was detected
between the two inoculation techniques employed. A general trend of accelerated
pupation following treatment with N. rileyi was noted with both inoculation tech-
niques (Hatting 2012). Among most of the cases, concentration is a critical para-
meter that determined the “speed of kill” of the exposed insect species. Kavallieratos
et al. (2014) tested I. fumosorosea, B. bassiana, and M. anisopliae against the
stored-grain pest Sitophilus oryzae. The mortality of S. oryzae adults during the
overall exposure period for the lowest, as well as for the highest, concentrations of
tested entomopathogenic fungi ranged from 0 to 100 %. Further they reported that
adult mortality of S. oryzae was higher when the entomopathogenic fungi were
directly applied on adults than fungus applied on food.
6.5.5 Entomopathogenic Fungus Lecanicillium
Species of Lecanicillium have a wide host range and have been isolated from a
variety of insect orders (Zare and Gams 2001; North et al. 2006; Anand et al. 2009).
Wang et al. (2007) tested crude toxins extracted from Lecanicillium (Verticillium)
lecanii (Zimmermann) strain V3450 and Vp28 for contact toxicity, feeding deter-
rence, and repellent activity against the sweet potato whitefly, Bemisia tabaci
(Gennadius). They reported that both toxins showed ovicidal activity. However,
nymphs of B. tabaci were the most susceptible stages and adults were the second
most susceptible stage. Both toxins exhibited repellent activity at low concentration
and antifeedant activity at high concentration. ChunLi et al. (2010) determined the
lethal concentration of L. lecanii strain MZ041024 against 2nd instar larvae of
Laphygma exigua and reported strong pathogenicity against pest. 100 % mortality
caused by L. lecanii was reported by Karthikeyan and Selvanarayanan (2011)
against cotton pests, Aphis gossypii and Bemisia tabaci.
Bio-Catch is a biological insecticide based on a selective strain of L. lecanii.
Product contains spores and mycelial fragments of L. lecanii and is available in
liquid (1 109 CFU/ml) and powder (1 108 CFU/gm) formulation. L. muscarium
has been commercialized as biopesticides, Mycotal against whiteflies and thrips
and Verticillin against whiteflies, aphids, and mites (Faria and Wraight 2007).
El-Hawary and Abd-El-Salam (2009) evaluated the efficacy of entomopathogenic
fungal commercial products, Bio-Power (B. bassiana), Bio-Catch (L. lecanii), and
Priority (Paecilomyces fumosoroseus), against S. littoralis larvae and found
Bio-Power to be the most effective product followed by Bio-Catch and Priority
with 87.5, 72.5, and 67.5 % larval mortality, respectively, at 1 109 spores/ml.
Oil-based formulation of mycoinsecticides could be an important biocontrol agent
in the management of lepidopteran pest. Sahayaraj and Borgio (2012) reported that
oil-based conidial formulations of L. lecanii, B. bassiana, and Paecilomyces
fumosoroseus were pathogenic to lepidopteran pest Pericallia ricini at all tested
concentrations. L. lecanii caused highest larval mortality followed by B. bassiana.
However, P. fumosoroseus caused least larval mortality.
6.5.6 Entomopathogenic Fungus Fusarium
The genus Fusarium comprises a large group of species of filamentous fungi widely
distributed in soil usually in association with plants. Most species are saprotrophic
and relatively abundant members of the soil microbiota (Leslie and Summerell
2006). Many Fusarium species are well known as pathogens of insects and plants.
More than 13 Fusarium species are pathogenic to insects, and the genus has a host
range that includes Lepidoptera, Coleoptera, Hemiptera, and Diptera (Teetor-
Barsch and Roberts 1983; Humber 1992). Fusarium includes various species/
strains that are able to produce potent secondary metabolites, such as trichothecenes
(Kilpatrick 1961; Kuno and Ferrer 1973), and fumonisins (Kuruvilla and Jacob
1979).
Ameen (2012) tested Fusarium isolates, F. chlamydosporum, F. equiseti,
F. graminearum, F. moniliforme, F. oxysporum, F. poae, F. semitectum,
F. sacchari, and F. solani isolated from soil samples of different locations and
reported pathogenicity of all Fusarium isolates against G. mellonella larvae. Rajesh
and Bhupendra (2009) reported 100 % mortality in S. litura unscaled eggs by the
infection of F. lateritium, M. anisopliae, and C. cardinalis at 106 conidia/ml. The
larvae were found susceptible to entomopathogenic fungi in a dose-dependent
manner. Further they noticed that when both larvae and the leaves (provided as
food) were treated with fungal conidia, mortality further increased. Zhang (2001)
isolated F. lateritium from dead insect bodies of citrus aphid. Bioassay results
indicated higher fungus virulence to the larvae of citrus aphid. Pelizza et al. (2011)
reported natural infection caused by F. verticillioides in grasshoppers. These results
strongly indicate the control potential of entomopathogenic fungi in managing
Spodopteran species.
6.6 Conclusions
It is well known that entomopathogenic fungi induced extensive epizootics among

caterpillar. These fungi have a wide host range and have been isolated from a
variety of insect orders. The potential of entomopathogenic fungi often vary in
different ecosystems and among fungal species and strains. Highly virulent fungal
genotypes against particular insect pest can be manipulated to develop new natural
insecticides. Current research efforts are directed at selective native entomo-
pathogenic fungi, their characterization, assessment of virulence, and developing
a formulation for them. Finally, entomopathogenic fungi have potential to be
implemented as a novel biocontrol agent against lepidopteran pests.
References
Abdel-Megeed MI (1975) Field observations on the vertical distribution of the cotton leafworm,
Spodoptera littoralis on cotton plants. Z Angew Entomol 78:597–662
Ahmad M, Arif MI, Ahmad M (2007a) Occurrence of insecticide resistance in field populations of
Spodoptera litura (Lepidoptera: Noctuidae) in Pakistan. Crop Prot 26:809–817
Ahmad M, Sayyed AH, Crickmore N, Saleem MA (2007b) Genetics and mechanism of resistance
to deltamethrin in a field population of Spodoptera litura (Lepidoptera: Noctuidae).
Pest Manag Sci 63:1002–1010
Ahmad M, Sayyed AH, Saleem MA (2008) Evidence for field evolved resistance to newer
insecticides in Spodoptera litura (Lepidoptera: Noctuidae) from Pakistan. Crop Prot 27:
1367–1372
Aktar MW, Sengupta D, Chowdhury A (2009) Impact of pesticide use in agriculture: their benefits
and hazards. Interdisc Toxicol 2:1–12
Alice SJ, Nadarajan L (2003) Occurrence of entomogenous fungi on rice pests on Karaikal region.
Insect Environ 9:192
Almudena OU, Nemat OK (2013) Action on the surface: entomopathogenic fungi versus the insect
cuticle. Insects 4:357–374
Alves SB, Pereira RM, Lopes RB, Tamai MA (2003) Use of entomopathogenic fungi in
Latin America. In: Upadhyay RK (ed) Advances in microbial control of insect pests.
Kluwer, New York, pp 193–212
Ambethgar V (2009) Potential of entomopathogenic fungi in insecticide resistance management
(IRM): A review. J Biopestic 2:177–193
Ameen MKM (2012) Screening of Fusarium isolates pathogenicity in vitro by using the larvae of
Galleria Mellonella L. J Basrah Res 38:19–28
Amer MM, El-Sayed TI, Bakheit HK, Moustafa SA, El-Sayed YA (2008) Pathogenicity and
genetic variability of five entomopathogenic fungi against Spodoptera littoralis. Res J Agric
Biol Sci 4:354–367
selected entomopathogenic fungi. BioControl 54:85–92
Anderson TE, Hajek AE, Roberts DW, Preisler K, Robertson JL (1989) Colorado potato beetle
(Coleoptera: Chrysomelidae): Effects of combinations of Beauveria bassiana with insecti-
cides. J Econ Entomol 82:83–89
Arivoli S, Samuel T (2012) Antifeedant activity of plant extracts against Spodoptera litura (Fab.)
(Lepidoptera: Noctiduae). Am-Euras J Agric Environ Sci 12:764–768
Arivoli S, Samuel T (2013) Antifeedant activity, developmental indices and morphogenetic
variations of plant extracts against Spodoptera litura (Fab) (Lepidoptera: Noctuidae).
J Entomol Zool Stud 1:87–96
Arivoli S, Tennyson S (2013) Ovicidal activity of plant extracts against Spodoptera litura (Fab)
(Lepidoptera: Noctuidae). Bull Environ Pharmacol Life Sci 2:140–145
Armes NJ, Wightman JA, Jadhav DR, Ranga RGV (1997) Status of insecticide resistance in
Spodoptera litura in Andhra Pradesh, India. Pesti Sci 50:240–248
Asi MR, Bashir MH, Afzal M, Ashfaq M, Sahi ST (2010) Compatibility of entomopathogenic
fungi, Metarhizium anisopliae and Paecilomyces fumosoroseus with selective insecticides.
Pak J Bot 42:4207–4214
Asi MR, Bashir MH, Afzal M, Khan BS, Khan MA, Gogi MD, Zia K, Arshad M (2012) Potential
of entomopathogenic fungi against larvae and eggs of Spodoptera litura (Lepidoptera:
Noctuidae). Pak Entomol 34:151–156
Asi MR, Bashir MH, Afzal M, Zia K, Akram M (2013) Potential of entomopathogenic fungi for
biocontrol of Spodoptera litura fabricius (lepidoptera: noctuidae). J Anim Plant Sci 23:
913–918
Balazy S (1993) Entomophthorales. In: Flora of Poland, fungi (Mycota), vol XXIV. Instytut
Botaniki, Krakow
Baskar K, Ignacimuthu S (2012a) Ovicidal activity of Atalantia monophylla (L) Correa against
Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). J Agric Technol 8:861–868
Baskar K, Ignacimuthu S (2012b) Antifeedant, larvicidal and growth inhibitory effect of ononitol
monohydrate isolated from Cassia tora L. against Helicoverpa armigera (Hub) and
Spodoptera litura (Fab.) (Lepidoptera: Noctuidae). Chemosphere 88:384–388
Baskar K, Maheshwaran R, Kingsley S, Ignacinuthu S (2011) Bioefficacy of plant extracts against
Asian army worm Spodoptera litura Fab. (Lepidoptera: Noctuidae). J Agric Technol 7:
123–131
Baskar K, Raj GA, Mohan PM, Lingathurai S, Ambrose T, Muthu C (2012) Larvicidal and
growth inhibitory activities of entomopathogenic fungus, Beauveria bassiana against Asian
Army worm, Spodoptera litura Fab. (Lepidoptera: Noctuidae). J Entomol 9:155
Basu AC (1981) Effect of different foods on the larval and post larval development of moth of
Prodenia litura (Fab.). J Bombay Nat Hist Soc 44:275–288
Batalha VC, Zanuncio JC, Picanco M, Guedes RNC (1997) Selectivity of insecticides to Podisus
nigrispinus (Heteroptera: Pentatomidae) and its prey Spodoptera frugiperda (Lepidoptera:
Noctuidae). Ceiba 38:19–22
Beckage NE (2008) Insect immunology. Academic Press/Elsevier, San Diego, p 348
Bhattacharya AK, Mondal P, Ramamurthy VV, Srivastava RP (2003) Beauveria bassiana: a
potential bioagent for innovative integrated pest management programme. In: Srivastava RP
(ed) Biopesticides and bioagents in integrated pest management of agricultural crops. Inter-
national Book Distributing Co, Lucknow, p 860
Bhatti SS, Ahmad M, Yousaf K, Naeem M (2013) Pyrethroids and new chemistry insecticides
mixtures against Spodoptera litura (Noctuidae: Lepidoptera) under laboratory conditions.
Asian J Agric Biol 1:45–50
Bhutia KC, Chakravarthy AK, Doddabasappa B, Narabenchi GB, Lingaraj VK (2012) Evaluation
and production of improved formulation of nucleopolyhedrosis virus of Spodoptera litura.
Boman HG, Hultmark D (1987) Cell-free immunity in insects. Annu Rev Microbiol 41:103–126
Boucias DG, Pendland JC (1991) Attachment of mycopathogens to cuticle: the initial event of
mycosis in arthropod hosts. In: Cole GT, Hoch HC (eds) The fungal spore and disease initiation
in plants and animals. Plenum, New York, pp 101–128
Burges HD (1981) Microbial control of pests and plant diseases 1970-1980. Academic, London.
ISBN 9780121433604
Butt TM, Jackson CW, Magan N (2001) Fungi as biocontrol agents: progress, problems and
potential. CAB International, Wallingford
Chari MS, Patel NG (1983) Cotton leaf worm Spodoptera litura (Fabr.): its biology and integrated
control measures. Cotton Dev 13:7–8
Charnley AK, Collins SA (2007) Entomopathogenic fungi and their role in pest control.
In: Kubicek CP, Druzhinina IS (eds) Environment and microbial relationships, 2nd edn.
The Mycota IV. Springer, Berlin, pp 159–185
Cho EM, Boucias D, Keyhani NO (2006a) EST analysis of cDNA libraries from the
entomopathogenic fungus Beauveria (Cordyceps) bassiana. II. Fungal cells sporulating on
chitin and producing oosporein. Microbiology 152:2855–2864
Cho EM, Liu L, Farmerie W, Keyhani NO (2006b) EST analysis of cDNA libraries from the
entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene
expression in aerial conidia, in vitro blastospores and submerged conidia. Microbiology
152(2843):2854
Cho EM, Kirkland BH, Holder DJ, Keyhani NO (2007) Phage display cDNA cloning and
expression analysis of hydrophobins from the entomopathogenic fungus Beauveria
(Cordyceps) bassiana. Microbiology 153:3438–3447
ChunLi X, ShengYong Y, Qiong K, Yali G, HongRui Z, ZengYue L (2010) Median lethal

concentration determination of Verticillium lecanii MZ041024 strain against 2nd instar larvae
of Laphygma exigua. Plant Dis pests 1:57–59
Clark PL, Molina O, Martinelli J, Skoda S, Isenhour SR, Lee DJ, Krumm JT, Foster JE (2007)
Population variation of the fall armyworm, Spodoptera frugiperda, in the Western hemisphere.
J Insect Sci 7:1–10
Clarkson JM, Charnley AK (1996) New insights into the mechanisms of fungal pathogenesis in
insects. Trends Microbiol 4:197–203
Contreras J, Mendoza JE, Martı́nez-Aguirre MR, Garcı́a-Vidal L, Izquierdo J, Bielza P (2014)
Efficacy of enthomopathogenic fungus Metarhizium anisopliae against Tuta absoluta (Lepi-
doptera: Gelechiidae). J Econ Entomol 107:121–124
Cuthbertson AGS, Murchie AK (2007) A review of the predatory mite Anystis baccarum and its
role in apple orchard pest management schemes in Northern Ireland. J Entomol 4:275–278
David I, James RF, Bruce WW, Khuong BN, Byson JA, Richard AH, Michael JH (2003) Survey of
entomopathogenic nematodes and fungi in pecan orchards of the southeastern United States
and their virulence to pecan weevil. Environ Entomol 5:187–195
Devi KU, Murali Mohan CH, Padmavathi J, Ramesh K (2003) Susceptibility to fungi of cotton boll
worms before and after a natural epizootic of the entomopathogenic fungus Nomuraea rileyi
(Hyphomycetes). Biocontrol Sci Technol 13:367–371
Dhaliwal GS, Koul O (2007) Biopesticide and pest management: conventional and biotechno-
logical approaches. Kalyani Publishers, New Delhi, p 455
Divyaa K, Sankarb M, Marulasiddeshac KN (2010) Efficacy of entomopathogenic nematode,
Heterorhabditis indica against three lepidopteran insect pests. Asian J Exp Biol Sci 1:183–188
Domenico P, Mayri D, Lesbia T, Blas D (2009) A granular formulation of Nomuraea rileyi farlow
(samson) for the control of Spodoptera frugiperda (lepidoptera: noctuidae). Interciencia
34:130–134
Domsch KH, Gams W, Anderson TH (1980) Compendium of soil fungi, vol 1. Academic, London,
p 893
Driver F, Milner RJ, Trueman WH (2000) A taxonomic revision of Metarhizium based on a
phylogenetic analysis of rDNA sequence data. Mycol Res 104:134–150
East DA, Edelson JV, Cartwright B (1989) Relative cabbage consumption by the cabbage looper
(Lepidoptera:Noctuidae), beet armyworm (Lepidoptera: Noctuidae), and diamond back moth
(Lepidoptera: Plutellidae). J Econ Entomol 82:1367–1369
Eilenberg J, Hajek A, Lomer C (2001) Suggestions for unifying the terminology in biological
control. BioControl 46:387–400
Elanchezhyan K (2006) Compatibility of Beauveria bassiana and Nomuraea rileyi with EPN for
control of Helicoverpa armigera. Ann Plant Prot Sci 14:64–68
El-Hawary FM, Abd El-Salam AME (2009) Laboratory bioassay of some entomopathogenic fungi
on Spodoptera littoralis (Boisd.) and Agrotis ipsilon (Hufn.) larvae (Lepidoptera: Noctuidae).
Egyptian Acad J Biol Sci 2:1–4
Enrique Q, Alain VEY (2004) Bassiacridin, a protein toxic for locusts secreted by the
entomopathogenic fungus Beauveria bassiana. Mycol Res 108:441–452
Evans HC (1989) Mycopathogens of insects of epigeal and aerial habitats. In: Wilding N,
Collins NM, Hammond PM, Weber JF (eds) Insect fungus interactions. Academic, London,
pp 205–238
Fan Y, Fang W, Guo S, Pei X, Zhang Y, Xiao Y, Li D, Jin K, Bidochka MJ, Pei Y (2007) Increased
insect virulence in Beauveria bassiana strains overexpressing an engineered chitinase.
Fang WG, Leng B, Xiao YH, Jin K, Ma JC, Fan YH, Feng J, Yang XY, Zhang YJ, Pei Y (2005)
Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve
fungal strain virulence. Appl Environ Microbiol 71:363–370
Faria MR, Wraight SP (2007) Mycoinsecticides and Mycoacaricides: a comprehensive list with
worldwide coverage and international classification of formulation types. Biol Control 43:
237–256
Federici BA, Bonning BC, St. Leger RJ (2008) Improvement of insect pathogens as insecticides
through genetic engineering. In: Hill C, Sleator R (eds) Pathobiotechnology. Landes Bioscience,
Austin, pp 15–40
Feng MG, Poprawski TJ, Khachatourians GG (1994) Production, formulation and application of
the entomopathogenic fungus Beauveria bassiana for insect control: current status. Biocontrol
Sci Technol 4:3–34
Ferron P (1978) Biological control of insect pests by entomopathogenic fungi. Annu Rev Entomol
23:409–442
Fletcher TB (1914) Some South Indian insects and other animals of importance considered
especially from an economic point of view. Government Press, Madras (presently Chennai),
p 565
Flexner JL, Belnavis DL (2000) Microbial insecticides. In: Rechcigl JE, Rechcigl NA (eds)
Biological and biotechnological control of insect pests, vol 1. Lewis Publishers Limited,
Boca Raton, pp 35–62
Freed S, Feng-Liang J, Naeem M, Shun-Xiang R, Hussian M (2012a) Toxicity of proteins secreted
by entomopathogenic fungi against Plutella xylostella (Lepidoptera: Plutellidae). Int J Agric
Biol 14:291–295
Freed S, Saleem MA, Khan MB, Naeem M (2012b) Prevalence and effectiveness of Metarhizium
anisopliae against Spodoptera exigua (Lepidoptera: Noctuidae) in Southern Punjab, Pakistan.
Pak J Zool 44:753–758
Freimoser FM, Screen S, Bagga S, Hu G, St. Leger RJ (2003) Expressed sequence tag (EST)
analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins
with potential activity in insect hosts. Microbiology 149:1–9
Fukatzu T, Sato H, Kuriyama H (1997) Isolation, inoculation to insect host, and molecular
phylogeny of an entomogenous fungus Paecilomyces tenuipes. J Invertebr Pathol 70:203–208
Fuxa JR (1998) Environmental manipulation for microbial control of insects. In: Babbosa P
(ed) Conservation biological control. Academic, San Diego, pp 255–289
Fuxa JR, Tanada Y (1987) Epizootiology of insect diseases. Wiley-Interscience, New York. ISBN
047187812X
Gelernter UD, Federici BA (1986) Isolation, identification and determination of virulence of a
nuclear polyhedrosis virus from the beet armyworm, Spodoptera exiqua (Lepidoptera:
Noctuidae). Environ Entomol 15:240–245
Gharib A (1979) Rahe pest in Khozestan. J Pestic Plant Pathol 47:161–178
Gillespie JP, Bailey AM, Cobb B, Vilcinskas A (2000) Fungi as elicitors of insect immune responses.
Arch Insect Biochem Physiol 44:49–68
Godonou I, James B, Atcha-Ahowe C, Vodouhe S, Kooyman C, Ahanchede A, Korie S (2009)
Potential of Beauveria bassiana and Metarhizium anisopliae isolates from Benin to control
Plutella xylostella L. (Lepidoptera: Plutellidae). Crop Prot 28:220–224
Golshan H, Saber M, Majidi-Shilsar F, Bagheri M, Mahdavi V (2013) Effects of common
pesticides used in rice fields on the conidial germination of several isolates of
entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin. J Entomol Res Soc 15:17
Gopalkrishnan C, Mohan S (2000) Effect of certain insecticides and fungicides on the conidial
germination of Nomuraea rileyi (Farlow) Samson. Entomon 25:217–223
Gothama AAA, Sikorowski PP, Lawrence GW (1995) Interactive effects of Steinernema
carpocapsae and Spodoptera exigua nuclear polyhedrosis virus on Spodoptera exigua larvae.
Gouge DH, Hague NGM (1993) Effects of Steinernema feltiae against sciarids infesting conifers
in a propagation house. Ann Appl Biol 122:184–185
Grewal PS, Richardson PN (1993) Effects of application rates of Steinernema feltiae (Nematoda:
Steinernematidae) on biological control of the mushroom fly, Lycoriella auripila (Diptera:
Sciaridae). Biocontrol Sci Tech 3:29–40
Guo HL, Ye BL, Yue YY, Chen QT, Fu CS (1986) Three new species of Metarhizium. Acta Mycol
Sinica 5:185–190
Gupta VP (2003) Natural occurrence of the entomopathogenic fungus Nomuraea rileyi in the
soybean green semilooper, Chrysodeixis acuta, in India. Online. Plant Health Prog. doi:10.
1094/PHP-2003-0113-01-HN
Gupta S, Wang Y, Jang HB (2005) Manduca sexta proPhenoloxidase activation requires
proPhenoloxidase activating proteinase (PAP) and serine protease homologues (HPSS) simul-
taneously. Insect Biochem Mol Biol 35:241–248
Gurmeet KB, Sanehdeep K (2013) Phenoloxidase activity in haemolymph of Spodoptera litura
(Fabricius) mediating immune responses challenge with entomopathogenic fungus, Beauveria
bassiana (Balsamo) Vuillmin. J Entomol Zool Stud 1:118–123
Hajek AE, Delalibera IJ (2010) Fungal pathogens as classical biological control agents against
arthropods. BioControl 55:147–158
Hajek AE, St Leger RJ (1994) Interactions between fungal pathogens and insect hosts. Annu Rev
Entomol 39:293–322
Hajek AE, Humber RA, Elkinton JS (1995) The mysterious origin of Entomophaga maimaiga in
North America. Am Entomol 41:31–42
Hall RA (1982) Deuteromycetes: virulence and bioassay design. In: Invertebrate pathology and
microbial control. Proceedings III international colloquium invertebrate pathology, XV
Annual Medium Soc. of Invertebrate Pathology, Brighton, pp 191–196
Hatting JL (2012) Comparison of three entomopathogenic fungi against the bollworm,
Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), employing topical vs per os inocu-
lation techniques. Afr Entomol 20:91–100
Hemasree E (2013) A critical review on the natural occurrence of entomopathogenic fungi in
agricultural ecosystem. Int J Appl Biol Pharma Technol 4:372–375
Hodge KT (2003) Clavicipitaceous anamorphs. In: White JF, Bacon CW, Hywel-Jones NL,
Spatafora JW (eds) Clavicipitalean fungi: evolutionary biology, chemistry, biocontrol and
cultural impacts. Marcel Dekker, New York, pp 75–123
Hu G, St. Leger RJ (2002) Field studies using a recombinant mycoinsecticide (Metarhizium
anisopliae) reveal that it is rhizosphere competent. Appl Environ Microbiol 68:6383–6387
Humber RA (1992) Collection of entomopathogenic fungi: catalog of strains. USDA-ARS Publi-
cation 110:1–177
Humber RA (1997) Fungi - Identification. In: Lacey L (ed) Manual of techniques in insect
pathology. Academic, London, pp 153–185
Hung SY, Boucias DG, Vey AJ (1993) Effect of Beauveria bassiana and Candida albicans on the
cellular defense response of Spodoptera exigua. J Invertebr Pathol 61:179–187
Hussain A, Tian MY, Ahmed S, Shahid M (2012) Current status of entomopathogenic fungi as
mycoinsecticides and their inexpensive development in liquid cultures, zoology. In: Garcia
M-D (ed) In Tech, pp 103–122. Available from: www.intechopen.com/books/zoology/current-
status-of-entomopathogenic-fungi-as-mycoinecticides-andtheir-inexpensive-development-in-
liq
Hussein HM, Zemek R, Habustova SO, Prenerova E, Adel MM (2013) Laboratory evaluation of a
new strain CCM 8367 of Isaria fumosorosea (syn. Paecilomyces fumosoroseus) on Spodoptera
littoralis (Boisd.). Arch Phytopath Plant Prot 46:1307–1319
Ignacimuthu IC (2008) Ecofriendly insect pest management. National symposium on ‘ecofriendly
insect pest management, Entomology Research Institute, Loyola College, Chennai. Curr Sci
94:10
Ignoffo CM, Garcia C, Hostetter DL, Pinnel RE (1977) Laboratory studies of the entomo-
pathogenic fungus Nomuraea rileyi: soil born contamination of soybean seedlings and dis-
persal of diseased larvae of Trichoplusia ni. J Invertebr Pathol 29:147–152
Inceoglu AB, Kamita SG, Hammock BD (2006) Genetically modified baculoviruses: a historical
overview and future outlook. Adv Virus Res 68:323–360
lepidopterous pests of soybean and green gram. J Appl Zool Res 15:160–162
Jackson TA, Alves SB, Pereira RM (2000) Success in biological control of soil dwelling insects by
pathogens and Nematodes. In: Gurr G, Wratten S (eds) Biological control: measures of
success. Kluwer Academic Dordrecht, The Netherlands, pp 271–296
Jandricic SE, Filotas M, Sanderson JP, Wraight SP (2014) Pathogenicity of conidia-based prepar-
ations of entomopathogenic fungi against the greenhouse pest aphids Myzus persicae, Aphis
gossypii, and Aulacorthum solani (Hemiptera: Aphididae). J Invertebr Pathol 118:34–46
Jayaraj S (1978) All India symposium on insect pests management-present, past and future: an
outlook (Abst.), Udaipur, pp 49–52
Joseph I, Edwin CD, Ranjit SAJA (2010) Studies on the influence of Beauveria bassania on
survival and gut flora of groundnut caterpillar, Spodoptera litura Fab. J Biopestic 3:553–555
Jotwani MG, Ray BK, Pradhan S (1961) Bioassay of the comparative toxicity of some insecticides
to the larvae of Prodenia litura Fabricius (Noctuidae: Lepidoptera). Ind J Entomol 23:50–53
Justin C, Leo G, Prem JJ, Jayasekhar M (2003) Comparative efficacy of Bacillus thuringiensis
Berliner formulations with insecticides against Plutella xylostella (L.) and their effect on
Cotesia plutellae Kurdj.on cauliflower. Agric Sci Digest 23:251–254
Kandibane M, Kumar K, Adiroubane D (2010) Effect of Bacillus thuringiensis Berliner formu-
lation against the rice leaf folder Cnaphalocrocis medinalis Guenee (Pyralidae: Lepidoptera).
J Biopestic 3:445–447
Karthikeyan A, Selvanarayanan V (2011) In vitro efficacy of Beauveria bassiana (Bals.)Vuill. and
Verticillium lecanii (Zimm.) viegas against selected insect pests of cotton. Recent Res Sci
Technol 3:142–143
Kaur S, Kaur HP, Kaur K, Kaur A (2011) Effect of different concentrations of Beauveria bassiana
on development and reproductive potential of Spodoptera litura (Fabricius). J Biopestic 4:
161–168
Kavallieratos NG, Athanassiou CG, Aountala MM, Kontodimas D (2014) Evaluation of the
entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea
for control of Sitophilus oryzae. J Food Prot 77:87–93
Kaya HK (1985) Susceptibility of early larval stages of Pseudaletia unipuncta and Spodoptera
exigua (Lepidoptera: Noctuidae) to the entomogenous nematode Steinernema feltiae
(Rhabditida: Steinernematidae). J Invertebr Pathol 46:58–62
Kaya HK (2006) Status of entomopathogenic nematodes and their symbiotic bacteria from
selected countries or regions of the world. Biol control 38:134–155
Kaya HK, Gaugler R (1993) Entomopathogenic nematodes. Annu Rev Entomol 38:181–206
Keller S, Zimmerman G (1989) Mycopathogens of soil insects. In: Wilding N, Collins NM,
Hammond PM and Webber JF. (Eds.) Insect-fungus interactions. Academic Press, New York,
pp 239–270
Khachatourians GG (1996) Biochemistry and molecular biology of entomopathogenic fungi.
In: Howard DH, Miller JD (eds) Human and animal relationships, Mycota VI. Springer,
Heidelberg, pp 331–363
Khachatourians GG, Sohail SQ (2008) Entomopathogenic fungi. In: Brakhage AA, Zipfel PF (eds)
Biochemistry and molecular biology, human and animal relationships, 2nd edn. The Mycota
VI. Springer, Berlin
Khan MA, Mumtaz R, Khan MA (2010) Management of Spilarctia obliqua through Bt and
chlorpyrifos combinations. Ann Plant Prot Sci 18:499–500
Kilpatrick RA (1961) Fungi associated with larvae of Sitona spp. Phytopathology 51:640–641
Kleespies RG, Huger AM, Zimmermann G (2008) Diseases of insects and other arthropods: results
of diagnostic research over 55 years. Biocontrol Sci Technol 18:439–484
Klingen I, Haukeland S (2006) The soil as a reservoir for natural enemies of pest insects and mites
with emphasis on fungi and nematodes. In: Eilenberg J, Hokkanen, HMT (eds) An ecological
and societal approach to biological control. Series: Progress in biological control, vol 2. Springer,
Heidelberg, pp 145–211
Knowles A (2005) New developments in crop protection product formulation. Agrow reports,
T & F Informa. UK Ltd. www.agrowreports.com
Korrat EEE, Abdelmonem AE, Helalia AA, Khalifa HMS (2012) Toxicological study of some
conventional and nonconventional insecticides and their mixtures against cotton leaf worm,
Spodoptera littoralis (Boisd.) (Lepidoptera: Noectudae). Ann Agric Sci 57:145–152
Kranthi KR, Jadhav DR, Wanjari RR, Ali SS, Russell D (2001) Carbamate and organophosphate
resistance in cotton pests in India, 1995 to 1999. Bull Entomol Res 91:37–46
Krasnoff SB, Watson DW, Gibson DM, Kwan EC (1995) Behavioral effects of the entomo-
pathogenic fungus, Entomophthora muscae on its host Musca domestica: postural changes in
dying hosts and gated pattern of mortality. J Insect Physiol 41:895–903
Krutmuang P, Prakongsuk S, Visitpanich J (2008) Selection of entomopathogenic fungi for
Spodoptera litura control. “Competition for resources in a changing world: New drive for
rural development” Tropentag, 7–9 October 2008, Hohenheim
Kubicek CP, Druzhinina IS (2007) Environmental and microbial relationships, 2nd edn.
The Mycota IV. Springer, Berlin, pp 159–187
Kulkarni NS, Lingappa S (2002) Bioefficacy of entomopathogenic fungus, Nomuraea rileyi
(Farlow) Samson on Spodoptera litura and Cydia ptychora in Soybean and on S. litura in
Potato. Karnataka J Agric Sci 15:47–52
Kumar V, Singh NP (2009) Spodoptera litura nuclear polyhedrosis virus (NPV-S) as a component
in integrated pest management (IPM) of Spodoptera litura (Fab.) on cabbage. J Biopestic 2:
84–86
Kuno G, Ferrer MAC (1973) Pathogenicity of two Fusarium fungi to an armoured scale insect
Selenaspidus articulatus. J Invertebr Pathol 22:473–474
Kurup AR, Joshi BG (1959) Investigation on the control of tobacco caterpillars (Prodenia litura F)
in nursery and field. Indian J Entomol 21:10–14
Kuruvilla S, Jacob A (1979) Comparative susceptibility of nymphs and adults of Nilaparvata
lugens to Fusarium oxysporum and its use in microbial control. Agric Res J Kerala 17:287–288
Lacey LA, Kaya HK (eds) (2000) Field manual of techniques in invertebrate pathology: appli-
cation and evaluation of pathogens for control of insects and other invertebrate pests.
Kluwer Academic, Dordrecht. ISBN 9781402059322
Lefroy HM (1908) The tobacco Catterpillar (Prudentia littoralis). Mem Dept Agric India Ent Ser
2:74–94
Lefroy HM (1909) Indian insect life: a manual of the insects of the plains (Tropical India).
Thacher & Co., Calcutta, p 786
St. Leger RJ (2008) Studies on adaptations of Metarhizium anisopliae to life in the soil. J Invertebr
Pathol 98:271–276
St. Leger RJ, Wang C (2009) Entomopathogenic fungi and the genomic era. In: Stock SP,
Vandenberg J, Glazer I, Boemare N (eds) Insect pathogens: molecular approaches and tech-
niques. CABI, Wallingford, pp 366–400
Lereclus D, Delecluse A, Lecadet MM (1993) Diversity of Bacillus thuringiensis toxins and genes.
In: Entwistle PF, Cory JS, Bailey MJ, Higgs S (eds) Bacillusthuringiensis, an environmental
biopesticide: theory and practice. John Wiley & Sons, Chichester, pp 37–69
Leslie JF, Summerell BA (2006) Fusarium verticilliodes (Saccardo) Nirenberg. In: Leslie JF,
Summerell BA (eds) The Fusarium Laboratory manual. Blackwell, Iowa, pp 274–279
Lezama GR, Hamm JJ, Molina ORJ, Lopez EM, Pescad RA, Eloise L (2001) Occurrence of
entomopathogens of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Mexican States of
Michoacan, Colima, Jalisco and Tamaulipas. Fla Entomol 84:23–30
Li W, Sheng C (2007) Occurrence and distribution of entomo-phthoralean fungi infecting aphids
in mainland China. Biocontrol Sci Technol 17:433–439
Liburd OE, Funderburk JE, Olson SM (2000) Effect of biological and chemical insecticides on
Spodoptera species (Lep: Noctuidae) and marketable yields of tomatoes. J Appl Entomol 124:
19–25
Li-chang T, Roger F (2004) Potential application of the entomopathogenic fungus, shape

Nomuraea rileyi, for control of the corn earworm, shape Helicoverpa armigera. J Entomol
Exp Appl 88:25–30
Lin HP, Yang XJ, Gao YB, Li SG (2007) Pathogenicity of several fungal species on Spodoptera
litura. Chin J Appl Ecol 18:937–940
Liu Y, Shen D, Zhou F, Wang G, An C (2014) Identification of immunity-related genes in Ostrinia
furnacalis against entomopathogenic fungi by RNA-Seq analysis. PLoS ONE 9(1):
e86436;1–24. doi:10.1371/journal.pone.0086436
Malarvannan S, Murali PD, Shanthakumar SP, Prabavathy VR, Nair S (2010) Laboratory evalu-
ation of the entomopathogenic fungi, Beauveria bassiana against the Tobacco caterpillar,
Spodoptera litura Fabricius (Noctuidae: Lepidoptera). J Biopest 3:126–131
Malo AR (1993) Estudio sobre la compatibilidad del hongo Beauveria bassiana (Bals.) Vuill.con
formulaciones comerciales de funguicidase insecticidas. Rev Colomb Entomol 19:151–158
Manjula K, Nagalingam B, Rao PA (2003) Occurrence of Nomuraea rileyi on Helicoverpa
armigera and Spodoptera litura in Guntur district of Andhra Pradesh. Ann Plant Prot Sci 11:
224–227
Maoye L, Shiguang L, Amei X, Huafeng L, Dexin C, Hui W (2014) Selection of Beauveria
isolates pathogenic to adults of Nilaparvata lugens. J Insect Sci 14:1–12
Mariapackiam S, Ignacimuthu S (2008) Larvicidal & Histopathological effects of oil formulation
on Spodoptera litura. In: Ignacimuthu S, Jeyarabbj S (eds) Recent trends in insect pest
management. Elite Publishing House Pvt. Ltd., New Delhi
Matsuura H, Naito A (1997) Studies on the cold-hardiness and overwintering of Spodoptera litura
F. (Lepidoptera: Noctuidae): VI. Possible overwintering areas predicted from meteorological
data in Japan. Appl Entomol Zool 32:167–177
May RM (2000) The dimension of life on earth. In: Raven PH, Williams T (eds) Nature and human
society: the quest for a sustainable world. National Academy Press, Washington, DC, pp 30–45
McClintock JT, Van-Beek NAM, Kough JL, Mendelsohn ML, Hutton PO (2000) Regulatory
aspects of biological control agents and products derived by biotechnology. In: Rechcigl JE,
Rechcigl NA (eds) biological and biotechnological control of insect pests, vol 1. Lewis
Publishers Limited, Boca Raton (EUA), pp 305–357
Meshrif WS, Rohlfs M, Hegazi MA, Barakat EM, Ai S, Shehata MG (2011) Interactions of
Spodoptera littoralis haemocytes following injection with the entomopathogenic fungi:
Beauveria bassiana and Nomuraea rileyi. J Egyptian Soc Parasitol 41:699–714
Meyling NV, Eilenberg J (2007) Ecology of the entomopathogenic fungi Beauveria bassiana and
Metarhizium anisopliae in temperate agro ecosystems: potential for conservation biological
control. Biol Control 43:145–155
Milner RJ (1997) Prospects for biopesticides for aphid control. Entomophaga 42:227–239
Ming X, Yun-Hong P, Hong-Tao W, Qing-Liang L, Tong-Xian L (2010) Effects of four host plants
on biology and food utilization of the cutworm, Spodoptera litura. J Insect Sci 10:1–22
Miranpuri GS, Khachatourians GG (1995) Application of Beauveria bassiana and Verticillium
lecanii against Saskatoon berry leaf aphid Acyrthosiphon macrosiphum. J Insect Sci 8:93–95
Mirhaghparast SK, Zibaee A, Hajizadeh J (2013) Effects of Beauveria bassiana and Metarhizium
anisopliae on cellular immunity and intermediary metabolism of Spodoptera littoralis
Boisduval (Lepidoptera: Noctuidae). Invertebr Surviv J 10:110–119
Miyahara Y, Wakikado T, Tanaka A (1971) Seasonal changes in the number and size of the
egg-masses of Prodenia litura. Jpn J Appl Entomol Zool 15:139–143
Moore D, Higgins PM, Lomer CJ (1996) The effects of simulated and natural sunlight on the
viability of conidia of Metarhizium flavoviride Gams and Rozsypal and interactions with
temperature. Biocontrol Sci Tech 7:87–94
Moraga QE, Carrasco DJA, Santiago AC (2006) Insecticidal and antifeedant activities of proteins
secreted by entomopathogenic fungi against Spodoptera littoralis (Lep., Noctuidae). J Appl
Entomol 130:442–452
Mudroncekova S, Marian M, Marek N, Ivan S (2013) Entomopathogenic fungus species

Beauveria bassiana (bals.) and Metarhizium anisopliae (metsch.) used as mycoinsecticide
effective in biological control of Ips typographus (L.). J Microbiol Biotechnol Food Sci 2:
2469–2472
Murlibaskaran MS, VenugopaL MS, Mahadevan NR (1997) Optical brighteners as UV protectants
and their influence on the virulence of nuclear polyhedrosis virus of Spodoptera litura
(Fabricius) (Lepidoptera : Noctuidae). J Biol Control 11:17–22
Murua GM, Molina OJ, Fidalgo P (2009) Natural distribution of parasitoids of larvae of the
fall armyworm, Spodoptera frugiperda, in Argentina. J Insect Sci 9:1–17
Muthukrishnan N, Ganapathy N, Nalini R, Rajendran R (2005) Pest management in horticultural
crops. New Madura Publishers, Madurai, p 325
Nandihalli BS, Patil BV, Somasekhar HP (1989) Influence of weather parameters on the popu-
lation dynamics of Spodoptera litura (Fb.) in pheromone and light traps. Karnataka J Agric Sci
2:62–67
Naser M, Bijan H, Rahim E, Alireza A, Azidah BAA, Rouhollah R (2013) Effect of
entomopathogenic fungi Beauveria bassiana (bals.) and Lecanicillium muscarium (petch) on
Trialeurodes vaporariorum westwood. Indian J Entomol 75:95–98
Neelapu NR, Reineke A, Chanchala UM, Koduru UD (2009) Molecular phylogeny of asexual
entomopathogenic fungi with special reference to Beauveria bassiana and Nomuraea rileyi.
Rev Iberoam Micol 26:129–145
Nethravathi CJ, Hugar PS, Krishnaraj PU, Vastrad AS, Awaknavar JS (2010) Bioefficacy of
native Sikkim Bacillus thuringiensis (Berliner) isolates against lepidopteran insects.
J Biopestic 3:448–451
Neuman G, Shields EJ (2004) Survey for entomopathogenic nematodes and fungi in alfalfa snout
beetle infested fields in Hungary and in New York state. Great Lakes Entomol 37:152–158
Nguya KM, Sunday E (2013) The use of entomopathogenic fungi in the control of tsetse flies.
Nguyen NC, Borgemeister HP, Zimmermann G (2007) Laboratory investigations on the potential
of entomopathogenic fungi for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae)
larvae and pupae. Biocontrol Sci Technol 17:853–864
North JP, Cuthbertson AGS, Walters KFA (2006) The efficacy of two entomopathogenic bio-
control agents against adult Thrips palmi (Thysanoptera: Thripidae). J Invertebr Pathol 92:
89–92
Oerke EC, Dehne HW (2004) Safeguarding production-losses in major crops and the role of
crop protection. Crop Prot 23:275–285
Ownley BH, Kimberly DG, Fernando EV (2010) Endophytic fungal entomopathogens with
activity against plant pathogens: ecology and evolution. BioControl 55:113–128
Pandey SN (1970) Ph.D. thesis submitted to University of Udaipur, India
Patil RK, Bhagat YS, Halappa B, Bhat RS (2013) Evaluation and characterization of
entomopathogenic fungus, Nomuraea rileyi (Farlow) Samson for the control of Spodoptera
litura (f.) and its compatibility with synthetic and botanical pesticides. Int J Recent Sci Res
4:2167–2172
Payne CC (1986) The control of insect pests by pathogens and insect-parasitic nematodes.
In: Proc Agrobiotic Conf on Advance Biotechnology and Agriculture, Bologna
Pelizza SA, Stenglein SA, Cabello MN, Dinolfo MI, Lange CE (2011) First record of Fusarium
verticillioides as an entomopathogenic fungus of grasshoppers. J Insect Sci 11:1–8
Pell JK, Eilenberg J, Hajek AE, Steinkraus DC (2001) Biology, ecology and pest management
potential of Entomophthorales. In: Butt TM, Jackson CW, Magan N (eds) Fungi as biocontrol
agents: progress, problems and potential. CABI International, Wallingford, pp 71–153
Petlamul W, Prasertsan P (2012) Evaluation of strains of Metarhizium anisopliae and Beauveria
bassiana against Spodoptera litura on the basis of their virulence, germination rate, conidia
production, radial growth and enzyme activity. Mycobiology 40:111–116
Powell KA, Jutsum AR (1993) Technical and commercial aspects of biocontrol products.
Pestic Sci 37:315–321
Prabagaran SR, Rupesh KR, Nimal SJ, Sudha Rani S, Jayachandran S (2003) Advances in pest
control: the role of Bacillus thuringiensis. Indian J Biotechnol 2:302–321
Purwar JP, Sachan GC (2005) Biotoxicity of Beauveria bassiana and Metarhizium anisopliae
against Spodoptera litura and Spilarctia oblique. Ann Plant Prot Sci 13:360–364
Purwar JP, Sachan GC (2006) Synergistic effect of entomogenous fungi on some insecticides
against Bihar hairy caterpillar Spilarctia obliqua (Lepidoptera: Arctiidae). Microbiol Res 161:
38–42
Quesada-Moraga E, Carrasco-Diaz JA, Santiago-Alvarez C (2006) Insecticidal and antifeedant
activities of proteins secreted by entomopathogenic fungi against Spodoptera littoralis (Lep.,
Noctuidae). J Appl Entomol 130:442–452
Rachappa V, Lingappa S, Patil RK (2007) Occurrence of entomopathogenic fungi in
Northern Karnataka. J Ecobiol 20:85–91
Rajesh A, Bhupendra NT (2009) Pathogenicity of entomopathogenic fungi to eggs and larvae of
Rajesh A, Birendra P, Bhupendra NT (2009) Relative susceptibility of Spodoptera litura pupae to
selected entomopathogenic fungi. BioControl 54:85–92
Ramakrishna N, Tiwari LD (1969) Polyhedrosis of Prodenia litura Fab. (Noctuidae: Lepidoptera).
Indian J Entomol 31:191–192
Ramiro ERN, Ramiro ARE, Juan MSY, Jaime MO, Steven RS, Roberto CR, René PR, Francisco
GH, John EF (2013) Occurrence of entomopathogenic fungi and parasitic nematodes on
Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae collected in central Chiapas, México.
Fla Entomol 96:498–503
Rangel DN, Braga GL, Anderson AJ, Roberts DW (2005) Variability in Richard JS, Neal TD,
Karl JK, Michael RK, 2010, Model reactions for insect cuticle sclerotization: participation of
amino groups in the cross-linking of Manduca sexta cuticle protein MsCP36. Insect Biochem
Mol Biol 40:252–258
Rath AC, Carr CJ, Graham BR (1995) Characterization of Metarhizium anisopliae strains carbo-
hydrate utilization (AP150CH). J Invertebr Pathol 65:152–161
Ravensberg WJ (1994) Biological control of pests: current trends and future prospects. In: Proc.
Brighton Crop Protection Conf., pests and diseases. British Crop Protection Council, Farnham,
pp 591–600
Raymond JSL, Chengshu W (2010) Genetic engineering of fungal biocontrol agents to achieve
greater efficacy against insect pests. Appl Microbiol Biotechnol 85:901–907
Rehner SA, Minnis D, Sung GH, Luangsa-ard JJ, DeVoto L, Humber RA (2011) Phylogeny and
systematics of the anamorphic, entomopathogenic genus Beauveria. Mycologia 103:
1055–1073
Rios-Velasco C, Cerna-Chavez E, Pena SS, Morales GG (2010) Natural epizootic of the
entomopathogenic fungus Nomuraea rileyi (Farlow) Samson infecting Spodoptera frugiperda
(Lepidoptera: Noctuidae) in Coahuila Mexico. J Res Lepidoptera 43:7–8
Roberts DW (1981) Toxins of entomopathogenic fungi. In: Burges HD (ed) Microbial control of
pests and plant diseases 1970-1980. Academic, London, pp 441–464
Roberts DW, Humber RA (1981) Entomogenous fungi. In: Cole GT, Kendrick B (eds) The biology
of conidial fungi, vol 2. Academic, New York, pp 201–236
Roberts DW, St. Leger RJ (2004) Metarhizium spp., cosmopolitan insect-pathogenic fungi:
mycological aspects. Adv Appl Microbiol 54:1–7
Rosell G, Quero C, Coll J, Guerrero A (2008) Biorational insecticides in pest management. J Pestic
Sci 33:103–121
Roy HE, Steinkraus DC, Eilenberg J, Hajek AE, Pell JK (2006) Bizarre interactions and endgames:
entomopathogenic fungi and their arthropod hosts. Annu Rev Entomol 51:331–357
Saad ASA, Madhkour A, El-Bahrwawi A (1975) Notes on the effect of insecticides on different
strains of Spodoptera littoralis Boisd. in Egypt. Indian J Agric Sci 45:231–232
Sadek MM (2003) Antifeedant and toxic activity of Adhatoda vasica leaf extract against
Spodoptera littoralis (Lepidoptera: Noctuidae). J Appl Entomol 27:396–404
Saeedeh J, Ahmad SS, Rozi M, Lau WH (2013) Suitability of Centella asiatica (Pegaga) as a food
source for rearing Spodoptera litura (F.) (Lepidoptera: Noctuidae) under laboratory conditions.
J Plant Prot Res 53:184–189
Sahayaraj K, Borgio JF (2012) Screening of some mycoinsecticides for the managing hairy
caterpillar, Pericallia ricini Fab. (Lepidoptera: Arctiidae) in castor. J Entomol 9:89–97
Sahayaraj K, Paulraj MG (1998) Screening the relative toxicity of some plant extracts to
Spodoptera litura Fab. (Insecta: Lepidoptera: Noctuidae) of groundnut. Fresenius Environ
Bull 7:557–560
Sahayaraj K, Namasivayam SKR, Rathi JM (2011) Compatibility of entomopathogenic fungi with
extracts of plants and commercial botanicals. Afr J Biotechnol 10:933–938
Salama HS, Shoukry A (1972) Flight range of the moth of the cotton leaf worm Spodoptera
littoralis. Zeitung Angew Entomol 71:181–184
Saleem MA, Ahmad M, Aslam M, Sayyed AH (2008) Resistance to selected organochlorine,
organophosphate, carbamate and pyrethroid, in Spodoptera litura (Lepidoptera: Noctuidae)
from Pakistan. J Econ Entomol 101:1667–1675
Samson RA, Evans HC, Latg’e JP (1988) Atlas of entomopathogenic fungi. Springer, Berlin, p 187
Sanchez-Pena SR (2000) Entomopathogens from two Chihuahuan desert localities in Mexico.
BioControl 45:63–78
Sarup P (1970) Effect of formulation on the toxicity of insecticidal dusts to the larva of
Prodenia litura Fabricius-1.Choice of diluents. Indian J Entomol 32:356–375
Sarup P, Singh DS (1974) Intra- and inter-specific variations in the effectiveness of diluents in
insecticidal dust formulations. Indian J Entomol 34:368–369
Satti AA, Gorashi NE (2013) Isolation and characterization of new entomopathogenic fungi from
the Sudan. Int J Sci Innov Disc 3:326–329
Serigo RSP, Jorge SJL, Raul F (2010) Occurrence of entomopathogenic fungi from agricultural
ecosystems in Saltillo, Mexico, and their virulence towards thrips and white flies. J Insect Sci
11:1
Sertkaya E, Bayram A, Kornosor S (2004) Egg and larval parasitoids of the beet armyworm
Spodoptera exigua on maize in Turkey. Phytoparasitica 32:305–312
Shankarganesh K, Walia S, Dhingra S, Subrahmanyam B, Babu SR (2012) Effect of
dihydrodillapiole on pyrethroid resistance associated esterase inhibition in an Indian popu-
lation of Spodoptera litura (Fabricius). Pestic Biochem Physiol 102:86–90
Sharma RK, Bisht RS (2008) Antifeedant activity of indigenous plant extracts against
Spodoptera litura Fabricius. J Insect Sci 21:56–60
Shaw KE, Davidson G, Clark SJ, Ball BV, Pell JK, Chandler D, Sunderland KD (2002) Laboratory
bioassay to assess the pathogenicity of microscopic fungi to Varroa destructor, an ectoparasitic
mite of the honey bee, Apis mellifera. Biol Control 24:266–276
Shereen ME, Nabawia ME, Fayez MS, Tayseer AR (2012) Physiological and biochemical effect of
entomopathogenic fungus Metarhizium anisopliae on the 5th instar of Schistcereca gregaria
(Orthoptera: Acrididae). J Res Environ Sci Toxicol 1:7–18
Shieh TR (1989) Industrial production of viral pesticides. Adv Virus Res 36:315–343
Sierotzki H, Camastral F, Shah PA, Aebi M, Tuor U (2000) Biological characteristics of selected
Erynia neoaphidis isolates. Mycol Res 104:213–219
Silva CAD (2000) Microorganismos entomopatogênicos associados a insetos e ácaros do
algodoeiro. Documentos. Campina Grande: EMBRAPA-CNPA 77, p 45
Sookar P, Bhagwant S, Awuor OE (2008) Isolation of entomopathogenic fungi from the soil and
their pathogenicity to two fruit fly species. J Appl Entomol 32:778–788
Sreedhar V, Devaprasad V (1995) Mycosis of Nomuraea rileyi in field populations of
Spodoptera litura in relation to four host plants. Indian J Entomol 58:192–195
Srisukchayakul P, Wiwat C, Pantuwatana S (2005) Studies on the pathogenesis of the local isolates
of Nomuraea rileyi against Spodoptera litura. Sci Asia 31:273–276
Steinhaus EA (1949) Principles of insect pathology. McGraw-Hill, New York, p 757

Steinhaus EA (1964) Microbial diseases of insects. In: DeBach P (ed) Biological control of insect
pests and weeds. Chapman and Hall, London, pp 515–547
Strasser H, Vey A, Butt TM (2000) Are there any risks in using entomopathogenic fungi for pest
control, with particular reference to the bioactive metabolites of Metarhizium, Tolypocladium
and Beauveria species? Biocontrol Sci Technol 10:717–735
Sun BD, Liu XZ (2008) Occurrence and diversity of insect-associated fungi in natural soils in
China. Appl Soil Ecol 39:100–108
Tamez-Guerra P, Castro-Franco R, Medrano-Roldan H, Mcguire MR, Galan-Wong LJ, Luna-
Olvera HA (1998) Laboratory and field comparisons of strains of Bacillus thuringiensis for
activity against noctuid larvae using granular formulations (Lepidoptera). J Econ Entomol 91:
86–93
Tanada Y, Kaya HK (1993) Insect Pathology. Academic, San Diego, 666
Teetor-Barsch GH, Roberts WD (1983) Fusarium species pathogens of insects review.
Mycopathologia 84:3–16
Thackar JRM (2002) An introduction to arthropod pest control. Cambridge University Press,
Cambridge, p 144
Thomas DB (1992) Taxonomic synopsis of the Asopinae pentatomidae (Heteroptera) of the
western hemisphere, vol 16. The Thomas Say Foundation, Entomological Society of America,
Lanham
Thomas MB, Read AF (2007) Can fungal biopesticides control malaria? Nature Rev Microbiol 5:
377–383
Tigano MS, Faria MR, Lecuona RE, Sartori MR, Arima EY, Diaz BM, De-Faria MR (1995)
Analysis of pathogenicity and germination of the fungus Nomuraea rileyi isolated in
Federal district. Anais Soc Entomol og Brasil 24:53–60
Tingle FC, Mitchell ER (1977) Seasonal populations of armyworms and loopers at Hastings,
Florida. Fla Entomol 60:115–122
Tong H, Su Q, Zhou X, Bai L (2013) Field resistance of Spodoptera litura (Lepidoptera:
Noctuidae) to organophosphates, pyrethroids, carbamates and four newer chemistry insecti-
cides in Hunan, China. J Pestic Sci 86:599–609
Torasco E, Poliseno M (2005) Preliminary survey on the occurrence of entomopathogenic
nematodes and fungi in Albanian soils. Bull OILB/SROP 28:165–168
Tucker MR (1983) Light-trap catches of African armyworm moths, Spodoptera exempta (Walker)
(Lepidoptera: Noctuidae), in relation to rain and wind. Bull Entomol Res 73:315–319
Udayababu P, Sunil Z, Goud CR (2012) Evaluation of entomopathogenic fungi for the manage-
ment of tobacco caterpillar Spodoptera litura (Fabricius). Indian J Plant Prot 40:214–220
Ujian AA, Shahzad S (2007) Pathogenicity of Metarhizium anisopliae Var. Acridum strains on
Pink Hibiscus Mealy bug (Maconellicoccus hirsutus) affecting cotton crop. Pak J Bot 39:
967–973
Uribe D, Khachatourians GG (2008) Identification and characterization of an alternative oxidase in
the entomopathogenic fungus Metarhizium anisopliae. Can J Microbiol 54:1–9
Vandenberg JD, Shelton AM, Wilsey WT, Ramos M (1998) Assessment of Beauveria bassiana
sprays for control of diamond back moth (Lepidoptera: Plutellidae) on crucifers. J Econ
Entomol 91:624–630
Vanninen I, Hokkanen H (1988) Effects of pesticides on four species of entomopathogenic fungi.
Annales Agriculturae Fennici 27:345–353
Veena KK, Rabindra RJ, Srinivasa NCD, Subha MR (2006) First report of Beauveria bassiana on
Amsacta albistriga Walker from Karnataka. Indian J Biol Control 20:95–96
Vey A, Hoagland R, Butt TM (2001) Toxic metabolites of fungal biocontrol agents. In: Butt TM,
Jackson CW, Magan N (eds) Fungi as biocontrol agents. CAB International, Wallingford,
pp 311–345
Vimaladevi PS (1994) Conidia production of the entomopathogenic fungus Nomuraea rileyi and
its evaluation for control of Spodoptera litura (Fab) on Ricinus communis. J Invertebr Pathol
63:145–150
Vimaladevi PS, Prasad YG (1997) The entomofungal pathogen Nomuraea rileyi. Info Bull Direc
Oilseeds Res, Hyderabad, p 3
Vimaladevi PS, Prasad YG (2001) Nomuraea rileyi: A potential mycoinsecticide. In: Upadyay
RK, Mukherji KG, Chamola BP (eds) Biocontrol potential and its exploitation in sustainable
agriculture insect Pests, vol 2. Kluwer Academic/Plenum, New York, pp 23–38
Vimaladevi PS, Prasad RD (2008) Isolation, maintenance and preservation techniques. In: Hands-on
training on microbial biocontrol agents of major insect pests and disease of crops, Hyderabad,
pp 14–21
Vimaladevi PS, Prasad YG, Chowdary DA, Mallikarjuna RL, Balakrishnan K (2003) Identifi-
cation of virulent isolates of the entomopathogenic fungus Nomuraea rileyi (F.) Samson for the
management of Helicoverpa armigera and Spodoptera litura. Mycopathologia 156:365–373
Vimaladevi PS, Prasad YG, Rajeswari B, Vijaya BL (1996) Epizootics of the entomofungal
pathogen, Nomuraea rileyi on lepidopterous pests of oil seed crops. J Oil Seeds Res 13:144–148
Vincent C, Goettel MS, Lazarovits G (2007) Biological control, a global perspective.
CABI, Oxfordshire
Vlak JH, Groner A (1980) Identification of two nuclear polyhedrosis viruses from the
cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae). J Invertebr Pathol 35:269–278
Vlak JH, Beider Eden, Peters D, Vrie V (1982) Bekampfung eines eingeschleppten Schadlings,
Spodoptera exiqua, in Gewachshausern mit dem autochtonen virus. Mededelingen. Faculteit.
Landbouwwetenschappen Rijksuniversiteit Gent 47:1005–1016
Wang CS, St. Leger RJ (2007a) A scorpion neurotoxin increases the potency of a fungal insecti-
cide. Nature Biotechnol 25:1455–1456
Wang CS, St. Leger RJ (2007b) The Metarhizium anisopliae perilipin homolog MPL1 regulates
lipid metabolism, appressorial turgor pressure, and virulence. J Biol Chem 282:21110–21115
Wang CS, St. Leger RJ (2007c) The MAD1 adhesin of Metarhizium anisopliae links adhesion with
blastospore production and virulence to insects, and the MAD2 adhesin enables attachment to
plants. Eukaryot Cell 6:808–816
Wang CS, Skrobek A, Butt TM (2004) Investigations on the destruxin production of the
entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 85:168–174
Wang S, Miao X, Zhao W, Huang B, Fan M, Li Z, Huang Y (2005) Genetic diversity and
population structure among strains of the entomopathogenic fungus, Beauveria bassiana,
as revealed by inter-simple sequence repeats (ISSR). Mycol Res 109:1364–1372
Wang L, Huang J, You M, Guan X, Liu B (2007) Toxicity and feeding deterrence of crude toxin
extracts of Lecanicillium (Verticillium) lecanii (Hyphomycetes) against sweet potato whitefly,
Bemisia tabaci (Homoptera: Aleyrodidae). Pest Manage Sci 63:381–387
Wheeler DA, Isman MB (2001) Antifeedant and toxic activity of Trichilia americana extract
against the larvae of Spodoptera litura. Entomol Exp Appl 98:9–16
Willcock FC (1905) Yaer book Khedivial. Agricultural Society, Egypt
Wilson JW (1934) The asparagus caterpillar: its life history and control. Fla Agric Exp St Bull 271:
1–26
Wraight SP, Carruthers RI, Bradley CA, Jaronski ST, Lacey LA, Wood P, Galaini-Wraight S
(1998) Pathogenicity of the entomopathogenic fungi Paecilomyces spp. and Beauveria
bassiana against the silver leaf whitefly, Bemisia argentifolii. J Invertebr Pathol 71:217–226
Wraight SP, Carruthers RI, Jaronski ST, Bradley CA, Garza CJ, Galaini-Wraight S (2000)
Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces
fumosoroseus for microbial control of the silver leaf whitefly, Bemisia argentifolii.
Wraight SP, Inglis GD, Goettel MS (2007) Fungi. In: Lacey LA, Kaya HK (eds) Field manual of
techniques in invertebrate pathology, 2nd edn. Springer, Dordrecht, pp 223–248. ISBN 978-1-
4020-5931-5
Wraight SP, Ramos ME, Avery PB, Jaronski ST, Vandenburg JD (2010) Comparative virulence of
Beauveria bassiana isolates against lepidopteran pests of vegetable crops. J Invertebr Pathol
103:186–199
Yang S, Yang S, Sun W, Jianping LV, Kuang R (2009) Use of sex Pheromone for control of
Spodoptera litura (Lepidoptera: Noctuidae). J Entomol Res Soc 11:27–36
Yathom S, Rivnay E (1960) Field trials against noctuidaes in cotton (In Hebraw). Rep Nat Inst
Agri Minist Serial No. 318, p 22
Yi Q, Sheng HY, Ying C, Zhi CS, Ming GF (2010) Integration of Insecticidal Protein Vip3Aa1
into Beauveria bassiana enhances fungal virulence to Spodoptera litura larvae by cuticle and
Per Os Infection. Appl Environ Microbiol 76:4611–4618
Zalom FG, Wilson LT, Hoffmann MP (1986) Impact of feeding by tomato fruitworm, Heliothis
zea (Boddie) (Lepidoptera: Noctuidae), and beet armyworm, Spodoptera exigua (Hübner)
(Lepidoptera: Noctuidae), on processing tomato fruit quality. J Econ Entomol 79:822–826
Zanuncio JC, Batalha VC, Guedes RNC, Picanco M (1998) Insecticide selectivity to Supputius
cincticeps (Stal) (Het.: Pentatomidae) and its prey Spodoptera frugiperda (J.E. Smith) (Lep.:
Noctuidae). J Appl Entomol 122:457–460
Zanuncio JC, da Silva CAD, de Lima ER, Pereira FF, Ramalho FDS, Serrao JE (2008) Predation
rate of Spodoptera frugiperda(Lepidoptera: Noctuidae) larvae with and without defense by
Podisus nigrispinus (Heteroptera: Pentatomidae). Braz Arch Biol Technol 51(1), Curitiba.
http://dx.doi.org/10.1590/S1516-89132008000100015
Zare R, Gams W (2001) A revision of Verticillium section. Prostata IV. The genera Lecanicillium
and Simplicillium gen.nov. Nova Hedwigia 73:1–50
Zemek R, Hussein HM, Prenerova E (2012) Laboratory evaluation of Isaria fumosorosea against
Spodoptera littoralis. Commu Agric Appl Biol Sci 77:685–689
Zhang S (2001) A species of entomogenous fungus Fusarium lateritium isolated from citrus aphid.
Scientia Silvae Sinicae 37:66–70
Zimmermann G (1986) The ‘Galleria bait method’ for detection of entomopathogenic fungi in soil.
J Appl Entomol 102:213–215
Zimmermann G (2007a) Review on Safety of the Entomopathogenic fungi Beauveria bassiana
and Beauveria brongniartii. Biocontrol Sci Technol 17:553–596
Zimmermann G (2007b) Review on safety of the entomopathogenic fungus Metarhizium anisopliae.
Biocontrol Technol 17:879–920
farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus):
biology, ecology and use in biological control. Biocontrol Sci Technol 18: 865–901
Chapter 7
Comparative Account of Generalist
and Specialist Species
of the Entomopathogenic Fungus,
Metarhizium
K. Sowjanya Sree and Hemesh Joshi
7.1 Introduction
Entomopathogenic fungi are one of the most extensively studied microorganisms

with respect to biological control of insect pests. They are one amongst the many
natural enemies of insect species worldwide (Hajek 2004; Kleespies et al. 2008).
There are around 750 entomopathogenic fungal species known on this planet,
amongst which species belonging to the genera Metarhizium (Pandey and Hasan
2009), Beauveria (Garcia et al. 2011), Nomuraea (Ingle et al. 2004), Lecanicillium
(Anand et al. 2009) and Isaria (Zimmermann 2008) have been well investigated.
These are also considered as model organisms for host–pathogen interaction studies
(Thomas and Read 2007). Their huge biodiversity allows nature as well as
researchers to choose a high virulent strain capable of killing the host in an effective
manner for use as biocontrol agent (Thomas and Read 2007). Much of the research
on these entomopathogenic fungi at present is focused on the factors determining
host specificity. In the present chapter, we will discuss on a few such recent findings
on the entomopathogenic fungal species belonging to the genus Metarhizium.
Metarhizium also known as the green muscardine fungus causes green
muscardine disease in their target insect hosts. A number of well-characterised
species are grouped under this genus; a few include Metarhizium anisopliae,
M. acridum, M. flavoviride, M. album and so on (Driver et al. 2000). Elie Metch-
nikoff in 1879 discovered M. anisopliae and named it as Oospora destructor and
also conducted the very first experiments on testing this fungus against a coleop-
teran grain beetle, Anisoplia austriaca. After realising the importance of biocontrol
agents, researchers started investigating this fungus from both application point of
view and for basic research on its infection process in the host and its host
K.S. Sree (*) • H. Joshi


DOI 10.1007/978-3-319-14499-3_7
162 K.S. Sree and H. Joshi
specificity (Wang et al. 2005; Anand and Tiwary 2009; Schrank and Vainstein
2010; Contreras et al. 2014). Metarhizium species have a worldwide distribution
from the tropics to the arctic regions and infect the insects and inhabit the soils in
varied climatic conditions like forests, coasts, swamps and deserts (Zimmerman
2007). Its life stages alternate between saprophytic phase in the soil and pathogenic
phase in the insect host. More recent studies showed that M. anisopliae colonises
plant roots, acting as both biopesticide and biofertiliser (St. Leger 2008).
7.2 Host Specificity of Metarhizium Species: Generalist vs

Specialist
The species of this genus exhibit a wide array of host specificity from being a broad
host range pathogen (e.g. M. anisopliae) to a very narrow host range pathogen
(e.g. M. acridum) (Driver et al. 2000). M. anisopliae is known to be pathogenic to
more than 100 insect pests, whereas its close relative M. acridum is known to infect
only locusts and grasshoppers (Zimmermann 1993; Driver et al. 2000; Peng
et al. 2008). M. acridum was earlier considered as a variety of M. anisopliae until
the investigations of Bischoff et al. (2009) came forward. They used a multigene
phylogenetic approach using nuclear-encoded gene regions of EF-1α, RPB1, RPB2
and β-tubulin together with the morphological markers. The phylogenetic evidence
from this study suggested the recognition of M. acridum at a species rank instead of
as a variety of M. anisopliae.
Recently, Gao et al. (2011) carried out very interesting investigation on these
fungal relatives. They made a comparative analysis of the genome and
transcriptome of M. anisopliae and M. acridum. Being very closely related, a
number of similarities were observed. Genomes of both the species were closer to
fungal pathogens and endophytes of plants than that of animals. This suggests that
Metarhizium might have evolved from plant fungal pathogens or endophytes. The
probable transition of fungi from plant to insect habitat might involve adaptation to
feed on insects. This was supported by the presence of huge number of genes for
proteases, lipases and chitinases which facilitate the digestion of insect cuticle and
body. The number of genes encoding secreted proteins was noticeably high in both
the species when compared to other plant fungal pathogens. These fungi produce a
whole set of proteases belonging to different types like subtilisins, chymotrypsins,
trypsins, metalloproteases, aspartyl proteases, cysteine proteases and exopepti-
dases; however, the chymotrypsins are specific to M. anisopliae. This might have
occurred via horizontal gene transfer (Screen and St. Leger 2000).
Although an array of proteases is produced by both the species of Metarhizium,
the levels are much too low in M. acridum when compared to M. anisopliae. The
high amounts of varied secreted proteases might allow M. anisopliae to survive in
different nutritional environments or, in other terms, in different hosts (Gao
et al. 2011). In addition, M. anisopliae encodes for dehydrogenases unlike
7 Comparative Account of Generalist and Specialist Species of the. . . 163
M. acridum and produces far more cytochrome P450s when compared to the latter
which might aid M. anisopliae to fight against a multitude of insect hosts.
Comparative transcriptome analysis revealed that M. anisopliae transcribed the
same G-protein-coupled receptors on cuticles of different hosts like locust and
cockroach, but M. acridum transcribed a G-protein-coupled receptor unique to its
host, i.e. a distinct receptor on cuticle of locust and another one on that of cockroach
(Gao et al. 2011). The comparative genome analysis also suggested that
M. anisopliae possesses greater potential to produce secondary metabolites than
M. acridum. More details will be discussed in the sections below.
7.2.1 Mechanical Kill of the Pest
Although there is a huge variation in the host range of the two species,
M. anisopliae and M. acridum, they both follow the same basic process of infecting
their host. When the spores come in contact with a susceptible host cuticle, they
adhere to the cuticle owing to the hydrophobic interactions between the
hydrophobins on the spore surface and the lipid layer on the host cuticle (Fang
et al. 2007). Germination of these conidia starts under suitable temperature and
humidity giving rise to a germ tube. They penetrate the host cuticle facilitated by
appressorium and penetration peg formation. Together with the help of turgor
pressure and the action of the cuticle-degrading enzymes as described above
(da Silva et al. 2005; Gao et al. 2011), the pathogen makes its way into the
haemolymph of the host where its hyphae proliferate absorbing nutrients from the
host body. The functions of several pathogenicity-related genes during this process
have been revealed recently (Wang and St. Leger 2006, 2007a, b; Duan et al. 2009;
Fang et al. 2010). Mycelium is septate and the hyphal network spreads throughout
the host body (Bechara et al. 2011), eventually killing the host through mechanical
injury. Later, the hyphae pierce out of the cadaver and sporulate resulting in
mycosis. The infection process is described in more details in Chap. 6 of this
volume.
7.2.2 Toxic Action of Secondary Metabolites
Destruxin (dtx) is one of the toxic secondary metabolites extracellularly secreted by

the fungus M. anisopliae. Chemically, it is cyclodepsipeptidic in nature comprising
of five amino acids and an α-hydroxy acid. The name destruxin was derived from
“destructor” from the species Oospora destructor (Metsch.) Delac., the
entomopathogenic fungus from which these metabolites were first isolated (Kodaira
1961). Later on O. destructor was renamed as M. anisopliae (Metsch.) Sorokin, but
as customary the compound’s trivial name was retained (Suzuki et al. 1970).
Individual dtxs differ on the hydroxy acid, N-methylation and R group of the
amino acid residues. Dtxs A, B and E have the same amino acid sequence (proline-
isoleucine-methyl valine-methyl alanine-beta alanine) but differ in the R group of
the hydroxy acid residue.
The EST analysis of M. anisopliae genome showed the presence of the peptide
synthases, reductases and other enzymes involved in the production of dtxs,
enniatins, trichothecenes and cytochalasins (Freimoser et al. 2003). Of all these,
dtxs have been widely exploited for their insecticidal activity. The great majority of
dtxs and its analogues were isolated from cultures of M. anisopliae (Pedras
et al. 2002), although this mycotoxin is produced by other fungi as well (Liu and
Tzeng 2012). Out of all the variants of this peptide produced in vitro, the most
predominant ones are dtxs A, B and E. Dtxs A and E have been proven to have
active insecticidal properties against different orders of insects (Dumas et al. 1994;
Pedras et al. 2002; Padmaja and Sree 2008). These mycotoxins which are extracel-
lularly secreted play an important role in pathogenesis (Kershaw et al. 1999).
Recently, the non-ribosomal peptide synthetase gene cluster coding for different
dtxs has been successfully revealed (Wang et al. 2012). Different strains of
M. anisopliae produce different amounts of dtxs in varying combinations (Sree
et al. 2008). However, its close relative and a specialist entomopathogen,
M. acridum, does not produce dtx (Wang et al. 2012). This is one of the major
differences between these two species. Dtxs suppress the host innate immune
response (Pal et al. 2007) and are also reported to induce oxidative stress in the
dtx-treated larvae (Sree and Padmaja 2008; Sree et al. 2010). The other cellular
effects of dtx on insects are briefed in Chap. 9 of this volume. Thus, the fungus
M. anisopliae kills its host by mechanical injury which is accompanied by the toxic
effects of dtx, whereas M. acridum kills its host only by mechanical injury. This is
also supported by the recent comparative genomic analysis of the two species (Gao
et al. 2011).
7.3 Manipulation of Host Range
In a recent study, Wang et al. (2011) demonstrated that expression of the gene
Mest1 coding for an esterase in the specialist M. acridum, transformed with this
gene, increased the host range of the fungus. In M. robertsii, under natural condi-
tions, MEST1, an esterase, is localised in the conidial lipid droplets. Mest1 expres-
sion mobilises the stored lipids, subjecting them to hydrolysis and thereby
supporting the germination and infection processes like appressorium formation.
It was found that Mest1 gene was upregulated in M. robertsii grown on Manduca
sexta cuticle-containing medium and its expression correlated with the virulence of
the fungus. This gene is reported to be absent in M. acridum. In the locust-specific
M. acridum when transformed with Mest1 gene from M. robertsii, it was found that
the transformant’s host range was broadened, and these transformants could now
infect and colonise lepidopteran caterpillars (Wang et al. 2011).
Two of the species of the entomopathogenic fungus Metarhizium, M. anisopliae

and M. acridum, differ considerably in their host range. Advancements in the fields
of genomics and transcriptomics led to an insight into the host specificity factors
involved in pathogenicity of Metarhizium. Nevertheless an in-depth understanding
of the factors inhibiting M. acridum from infecting insects other than locusts and
grasshoppers will be highly appreciated and will facilitate the development and
improvement of broad- and narrow-range bio-insecticides.
Acknowledgements KSS is grateful to SERB, Govt. of India, for financial assistance through
the Fast Track Young Scientist scheme.
References

selected entomopathogenic fungi. Biocontrol 54:85–92
Bechara IJ, Destefano RHR, Bresil C, Messias CL (2011) Histopathological events and detection
of Metarhizium anisopliae using specific primers in infected immature stages of the fruit fly
Anastrepha fraterculus (Wiedemann, 1830) (Diptera: Tephritidae). Braz J Biol 71:91–98
Bischoff JF, Rehner SA, Humber RA (2009) A multilocus phylogeny of the Metarhizium
anisopliae lineage. Mycologia 101:512–530
Contreras J, Mendoza JE, Martı́nez-Aguirre MR, Garcı́a-Vidal L, Izquierdo J, Bielza P (2014)
Efficacy of enthomopathogenic fungus Metarhizium anisopliae against Tuta absoluta (Lepi-
doptera: Gelechiidae). J Econ Entomol 107:121–124
da Silva MV, Santi L, Staats CC, da Costa AM, Colodel EM, Driemeier D, Vainstein MH, Schrank
A (2005) Cuticle-induced endo/exoacting chitinase CHIT30 from Metarhizium anisopliae is
encoded by an ortholog the chi3 gene. Res Microbiol 156:382–392
Driver F, Milner JR, Trueman HWJ (2000) A taxonomic revision of Metarhizium based on a
phylogenetic analysis of rDNA sequence data. Mycol Res 104:134–150
Duan ZB, Shang YF, Gao Q, Zheng P, Wang CS (2009) A phosphoketolase Mpk1 of bacterial
origin is adaptively required for full virulence in the insect-pathogenic fungus Metarhizium
anisopliae. Environ Microbiol 11:2351–2360
Dumas C, Rober P, Pais M, Vey A, Quiot JM (1994) Insecticidal and cytotoxic effects of natural
and hemisynthetic destruxins. Comp Biochem Physiol C 108:195–203
Fang W, Pei Y, Bidochka MJ (2007) A regulator of a G protein signalling (RGS) gene, cag8, from
the insect-pathogenic fungus Metarhizium anisopliae is involved in conidiation, virulence and
hydrophobin synthesis. Microbiology 153:1017–1025
Fang W, Fernandes EK, Roberts DW, Bidochka MJ, St. Leger RJ (2010) A laccase exclusively
expressed by Metarhizium anisopliae during isotropic growth is involved in pigmentation,
tolerance to abiotic stresses and virulence. Fungal Genet Biol 47:602–607
Freimoser FM, Screen S, Bagga S, Hu G, St Leger RJ (2003) Expressed sequence tag (EST)
analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins
with potential activity in insect hosts. Microbiology 149:239–247
Gao Q, Jin K, Ying SH, Zhang Y, Xiao G et al (2011) Genome sequencing and comparative
transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum.
PLoS Genet 7:e1001264
Hajek A (2004) Natural enemies: an introduction to biological control. Cambridge University
Press, Cambridge
lepidopterous pests of soybean and green gram. J Appl Zool Res 15:160–162
Kershaw MJ, Moorhouse ER, Bateman RP, Reynolds SE, Charnley AK (1999) The role of
destruxins in the pathogenicity of Metarhizium anisopliae for three species of insect. J
Invertebr Pathol 74:213–223
Kleespies RG, Huger AM, Zimmermann G (2008) Diseases of insects and other arthropods: results
of diagnostic research over 55 years. Biocontrol Sci Technol 18:439–484
Kodaira Y (1961) Biochemical studies on the muscardine fungi in the silkworms, Bombyx mori
L. J Fac Text Sci Technol Sinshu Univ 5:1–68
Liu BL, Tzeng YM (2012) Development and applications of destruxins: a review. Biotechnol Adv
30:1242–1254
Padmaja V, Sree KS (2008) Role of mycotoxin from the entomopathogenic fungus, Metarhizium
anisopliae for insect pest management- current status. In: Ignacimuthu sj S, Jayaraj S (eds)
Recent trends in insect pest management. Elite Publishers, New Delhi, pp 146–154
Pal S, St. Leger RJ, Wu LP (2007) Fungal peptide destruxin a plays a specific role in suppressing
the innate immune response in Drosophila melanogaster. J Biol Chem 282:8969–8977
Peng GX, Wang ZK, Yin YP, Zeng DY, Xia YX (2008) Field trials of Metarhizium anisopliae var.
acridum (Ascomycota: Hypocreales) against oriental migratory locusts, Locusta migratoria
manilensis (Meyen) in Northern China. Crop Prot 27:1244–1250
Schrank A, Vainstein MH (2010) Metarhizium anisopliae enzymes and toxins. Toxicon 56:1267–
1274
Screen SE, St. Leger RJ (2000) Cloning, expression, and substrate specificity of a fungal chymo-
trypsin. Evidence for lateral gene transfer from an actinomycete bacterium. J Biol Chem
275:6689–6694
Sree KS, Padmaja V, Murthy LNY (2008) Insecticidal activity of destruxin, a mycotoxin from
Metarhizium anisopliae (Hypocreales), against Spodoptera litura (Lepidoptera: Noctuidae)
larval stages. Pest Manag Sci 64:119–125
Sree KS, Sachdev B, Padmaja V, Bhatnagar RK (2010) Electron spin resonance spectroscopic
studies of free radical generation and tissue specific catalase gene expression in Spodoptera
litura (Fab.) larvae treated with the mycotoxin, destruxin. Pestic Biochem Physiol 97:168–176
St. Leger RJ (2008) Studies on adaptations of Metarhizium anisopliae to life in the soil. J Invertebr
Pathol 98:271–276
Suzuki A, Taguchi H, Tamura S (1970) Isolation and structure elucidation of three new insecti-
cidal cyclodepsipeptides, destruxin C, D and desmethyl-destruxin B, produced by Metarhizium
anisopliae. Agric Biol Chem 34:813–816
Thomas MB, Read AF (2007) Can fungal biopesticides control malaria? Nat Rev Microbiol
5:377–383
Wang C, St. Leger RJ (2006) A collagenous protective coat enables Metarhizium anisopliae to
evade insect immune responses. Proc Natl Acad Sci USA 103:6647–6652
Wang C, St. Leger RJ (2007a) The MAD1 adhesin of Metarhizium anisopliae links adhesion with
blastospore production and virulence to insects, and the MAD2 adhesin enables attachment to
plants. Eukaryot Cell 6:808–816
Wang C, St. Leger RJ (2007b) The Metarhizium anisopliae perilipin homolog MPL1 regulates
lipid metabolism, appressorial turgor pressure, and virulence. J Biol Chem 282:21110–21115
Wang C, Butt TM, St. Leger RJ (2005) Colony sectorization of Metarhizium anisopliae is a sign of
ageing. Microbiology 151:3223–3236
Wang S, Fang W, Wang CS, St Leger RJ (2011) Insertion of an esterase gene into a specific locust
pathogen (Metarhizium acridum) enables it to infect caterpillars. PLoS Pathog 7:e1002097
Wang B, Kang Q, Lu Y, Bai L, Wang C (2012) Unveiling the biosynthetic puzzle of destruxins in
Metarhizium species. Proc Natl Acad Sci USA 109:1287–1292
Zimmerman G (2007) Review on safety of the entomopathogenic fungus Metarhizium anisopliae.
Zimmermann G (1993) The entomopathogenic fungus Metarhizium anisopliae and its potential as
a biocontrol agent. Pestic Sci 37:375–379
farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces
fumosoroseus): biology, ecology and use in biological control. Biocontrol Sci Technol
18:865–901
Chapter 8
Non-ribosomal Peptides from Entomogenous
Fungi
Qiongbo Hu and Tingyan Dong
8.1 Introduction
Entomogenous fungi, the pathogenic fungi of insects, are ubiquitous in natural

environment. They play a very important role in controlling the natural population
of insect pests. Many of their species (e.g., Beauveria bassiana, Metarhizium
anisopliae) have been developed as myco-insecticides worldwide. Entomogenous
fungi are able to produce mycotoxins such as non-ribosomal peptides (NRPs) which
are toxic to tissues of host insects. Chemically, NRPs are a kind of secondary
compounds biosynthesized by non-ribosomal peptide synthetase (NRPS) existing
widely in fungi. NRPS gene of fungi is an open reading frame encoding a peptide
chain composed of several modules, which activate amino acids and combined with
a specific peptide product. Each module has a number of domains, and a specific
reaction is catalyzed by one domain. The main domains include: adenylation
domains (A domains), thiotion domains (T domains), condensation domains
(C domains), epimerization domains (E domains), and methylation domains
(M domains). The enzyme has been documented in many literature reviews
(Boettger and Hertweck 2013; Hur et al. 2012).
Entomogenous fungi produce various kinds of NRPs, and each NRP consists of
many analogues. According to the different molecular structures, entomogenous
fungal NRPs could be divided into chain peptides (e.g., cicadapeptin and
efrapeptin) and cyclic peptides including a subdivision of cyclopeptides and
cyclodepsipeptides. Cyclopeptides are cyclic structures built by amino acid resi-
dues through peptide bonding (e.g., cyclosporin), while cyclodepsipeptides are
lactone compounds consisting of amino acids and hydroxyl acids which are
connected by peptide bonds. Most of the NRPs such as destruxin, beauvericin,
Q. Hu (*) • T. Dong
College of Agriculture, South China Agricultural University, Guangzhou 510642, China

DOI 10.1007/978-3-319-14499-3_8
170 Q. Hu and T. Dong
enniatin, bassianolide, beauverolide, and serinocyclin belong to the group,

cyclodepsipeptides.
8.2 Chain Peptides
8.2.1 Cicadapeptins
Cicadapeptins were isolated from the entomogenous fungi Cordyceps heteropoda

(ARSEF #1880) and Isaria sinclairii (Krasnoff et al. 2005; Nagaoka et al. 2006). It
was structurally elucidated that cicadapeptins I and II are acylated at the N-terminus
by n-decanoic acid and amidated at the C-terminus by 1,2-diamino-4-
methylpentane. The amino acid sequence of cicadapeptin I is N-terminus-Hyp-
Hyp-Val-Aib-Gln-Aib-Leu-C-terminus, while in cicadapeptin II the Leu residue is
replaced by Ile substitutes (Krasnoff et al. 2005) (Fig. 8.1 and Table 8.1).
Cicadapeptins inhibited the acetylcholine (ACh)-evoked secretion of catechol-
amines in a concentration-dependent manner (Nagaoka et al. 2006). Cicadapeptins
I and II showed antibacterial activity against Bacillus cereus, B. subtilis, and
Escherichia coli (Rivas et al. 2008).
O NH2
R1
R2
O O O
(R)
H H H
(S) N (S) N (S ) N
(S) N 4 N 3 N 2
HO 5 H H H 1 (S)
(R) N O O O
NH2
O
(S) 6
N
HO (R)
O
Fig. 8.1 Structure of cicadapeptins
Table 8.1 Analogues of cicadapeptins

Analogues Molecular Geometry
name CAS registry no. formula configuration R1 R2
Cicadapeptin I 845626-76-8 C50H90N10O11 1S, 2S, 3S, 4S, 5S, 6S Me H
Cicadapeptin II 845626-81-5 C50H90N10O11 1S, 2S, 3S, 4S, 5S, 6S H Me
Note: Me, –CH3
8 Non-ribosomal Peptides from Entomogenous Fungi 171
O OH
(R )
O O R3 R4 O
H H
5 4 N N 2 O
( S)
N (S) N N ( S)
(S) H H 3 H
N O R1 R2 O HN
O
O NH
O ( S)
1
H
HO N NH
N
H
O
Fig. 8.2 Basic structure of culicinins
8.2.2 Culicinins
Culicinins were isolated from Culicinomyces clavisporus, a fungal pathogen of a

wide range of mosquito larvae (He et al. 2006). The chemistry, structure, and
synthesis of culicinins were studied by Zhang et al. (2008, 2009, 2011) (Fig. 8.2
and Table 8.2). The major analogue, anticancer agent culicinin D, was tested in
oncology assays and exhibited selective inhibitory activity against PTEN-negative
MDA468 breast tumor cells versus PTEN-positive MDA468 cells (He et al. 2006).
8.2.3 Efrapeptins and Neoefrapeptins
Efrapeptin was firstly discovered in the 1970s and was originally named A23871
(Lardy et al. 1975). They were isolated from Tolypocladium and other fungal
species (Jackson et al. 1979). There are ten efrapeptin analogues known till date
(Fig. 8.3 and Table 8.3). Structurally, efrapeptins are rich in Cα-dialkyl amino acids
such as α-aminoisobutyric acid (Aib) or isovaline (Iva) and contain one β-alanine
and several pipecolic acid residues (Krasnoff and Gupta 1991; Hayakawa
et al. 2008; Boot et al. 2007). The C-terminus bears an unusual heterocyclic cationic
cap. All efrapeptins were shown to adopt helical conformations in solvent (Weigelt
et al. 2012).
Efrapeptins have insecticidal activity against Tetranychus telarius, Musca
domestica, Leptinotarsa decemlineata, Tetranychus urticae, Helicoverpa assulta,
etc. (Krasnoff et al. 1991). Efrapeptins have anti-immune action suppressing
Table 8.2 Analogues of culicinins

Analogues CAS registry Molecular Geometry
name no. formula configuration R1 R2 R3 R4
Culicinin A 889128-37-4 C61H111N11O13 1S, 2S, 3S, 4S, 5S H Me H Me
Culicinin B 889128-38-5 C62H113N11O13 1S, 2S, 3S, 4S, 5S Me Me Me H
Culicinin C 889128-39-6 C62H113N11O13 1S, 2S, 3S, 4S, 5S H Me Me Me
Culicinin D 889128-40-9 C63H115N11O13 1S, 2S, 3S, 4S, 5S Me Me Me Me
a b Ac
O i-Bu R3 O O N
H O O H
O N NH N (S)
8
N 7 N 9(S) N 10
H 6 (S) H
HN O R4
O
H O
N
O N O O i-Bu O i-Bu
H H H
H O H N 3 N (S) 1
O N 5 4
N N N N (S) N (S)
+ (S) H H 2 H
R N N +
H R1 N
O R2 O
i-Bu i-Bu
N N
Fig. 8.3 Basic structure of efrapeptins; (a) efrapeptins A–B and (b) efrapeptins C–G
agglutination in Galleria mellonella, and this may be a result of its interference in

the ligand-receptor interactions at the membrane of specific hemocytes (Bandani
2004). They also exhibit antifungal activities against M. anisopliae and
Tolypocladium niveum (Krasnoff et al. 1991).
Efrapeptins are known as inhibitors of F1F0-ATPase, a mitochondrial enzyme
(Papathanassiu et al. 2006). The breast cancer cells treated with efrapeptins show a
disruption of the Hsp90:F1F0-ATPase complex and inhibition of Hsp90 chaperone
activity (Papathanassiu et al. 2011). Efrapeptins displayed potent cytotoxicity
against murine cancer cell lines and also demonstrated antibacterial properties
(Boot et al. 2007). It was found that efrapeptin F had preferential cytotoxicity to
nutrient-deprived cells compared to nutrient-sufficient cells. Efrapeptin F acts as a
mitochondrial complex V inhibitor (Momose et al. 2010). Efrapeptins F, G, and J
inhibited, in a dose-dependent manner, the 2-deoxyglucose-induced luciferase
expression in HT1080 human fibrosarcoma cells transfected with a luciferase
reporter plasmid containing the GRP78 promoter. Efrapeptin J also inhibited the
protein expression of GRP78 in HT1080 cells and MKN-74 human gastric cancer
cells (Hayakawa et al. 2008).
Neoefrapeptins, another chain peptide structurally similar to efrapeptins, were
recently isolated from Geotrichum candidum (Molleyres et al. 2004; Fredenhagen
et al. 2006) and displayed insecticidal activities. The structures of neoefrapeptins A
to N have been elucidated (Fredenhagen et al. 2006; De Zotti et al. 2012) (Fig. 8.4
and Table 8.4).
Table 8.3 Analogues of efrapeptins
Analogues CAS registry Molecular
name no. formula Geometry configuration R R1 R2 R3 R4
Efrapeptin A 138145-52-5 C32H58N7O5 – –COCH2NHCOC – – – –
(CH3)2NHAc
Efrapeptin B 138145-53-6 C26H48N5O3 – –Ac – – – –
Efrapeptin C 138145-54-7 C80H137N18O16 1S, 3S, 5S, 6S, 9S, 10S – Me H Me Me
Efrapeptin D 71503-60-1 C81H139N18O16 1S, 2S, 3S, 5S, 6S, 9S, 10S – Et H Me Me
Efrapeptin E 138168-07-7 C82H141N18O16 1S, 2S, 3S, 5S, 6S, 8S, 9S, 10S – Et H Me Et
8 Non-ribosomal Peptides from Entomogenous Fungi
Efrapeptin F 131353-66-7 C82H141N18O16 1S, 2S, 3S, 4S, 5S, 6S, 9S, 10S – Et Me Me Me
Efrapeptin G 138145-55-8 C83H143N18O16 1S, 2S, 3S, 4S, 5S, 6S, 8S, 9S, 10S – Et Me Me Et
Efrapeptin H 138264-31-0 C84H145N18O16 1S, 2S, 3S, 4S, 5S, 6S, 7S, 8S, 9S, 10S – Et Me Et Et
Efrapeptin J 1058669-41-2 C81H139N18O16 1S, 3S, 4S, 5S, 6S, 9S, 10S – Me Me Me Me
Note: Ac, HOOC–
173
O R3 O O
O H O H i-Bu
O N R1 N
N N 2 N NH
1 H R4 H
N R2
H
O
N R6
Ac H H
N O 4 N O N O 3
O
R8 R7 N O N O R5 N
H O N
H
H H
Fig. 8.4 Basic structure of neoefrapeptins
8.3 Cyclodepsipeptides
8.3.1 Bassianolides
Bassianolides were first isolated in the 1970s; they are reported to be produced by
B. bassiana and Verticillium lecanii (Suzuki et al. 1977; Kanaoka et al. 1978)
(Fig. 8.5 and Table 8.5). Bassianolide analogues are octadepsipeptidic derivatives
with a 24-membered macrolactone ring that is formed as the cyclic tetrameric ester
of the dipeptidol monomer, D-hydroxyisovalerate (D-Hiv)-N-methyl-L-leucine (N-
Me-Leu) (Xu et al. 2009). Bassianolides could inhibit muscle contraction (Nakajyo
et al. 1982) and kill silkworm when fed with an artificial diet containing 13 ppm of
bassianolides (Suzuki et al. 1977). Bassianolides had no influence on intracellular
Na+ and K+ contents and, therefore, might not be ionophoric (Nakajyo et al. 1983).
Bassianolide analogues also have anthelmintic efficacy against the parasitic nem-
atode Ascaridia galli in chicken (Ohyama et al. 2011). Bassianolide synthetases
(348 kDa) from Beauveria bassiana ATCC 7159 was reconstituted in Saccharo-
myces cerevisiae BJ5464-NpgA, leading to the production of bassianolide
(Yu et al. 2013b; Yu et al. 2013a).
8.3.2 Beauvericins
Beauvericins were first isolated in the 1960s (Hamill et al. 1969). They were
produced by the entomogenous fungal genera, Beauveria, Paecilomyces, Fusarium,
etc. (Bernardini et al. 1975; Gupta et al. 1991, 1995; Plattner and Nelson 1994).
Chemically, beauvericins are a kind of cyclic hexadepsipeptide with alternating
methyl-phenylalanyl and hydroxy-isovaleryl residues (Figs. 8.6 and 8.7, Tables 8.6
and 8.7).
Beauvericins G1–G3 and H1–H3 were biosynthesized and isolated from
B. bassiana ATCC 7159 cultured with analogues of D-2-hydroxyisovalerate and
L-phenylalanine. Beauvericins G1–G3 caused a parallel decline of cell migration
Table 8.4 Analogues of neoefrapeptins
Analogues name CAS registry no. Molecular formula Geometry configuration R1 R2 R3 R4 R5 R6 R7 R8
Neoefrapeptin A 695200-77-2 C82H139N18O16 1R, 4S sBu H Me Me Me Me iBu Rx
Neoefrapeptin B 695200-79-4 C83H141N18O16 1R, 2R, 4S sBu H sBu H Me Me iBu Rx
Neoefrapeptin C 695200-81-8 C83H141N18O16 1R, 3S, 4S sBu H Me Me sBu H iBu Rx
Neoefrapeptin D 695200-83-0 C81H137N18O16 4S Me Me Me Me Me Me iBu Rx
Neoefrapeptin E 695200-85-2 C84H143N18O16 1R, 2R, 3S, 4S sBu H sBu H sBu H iBu Rx
Neoefrapeptin F 695200-87-4 C82H139N18O16 – sBu H Me Me Me Me iBu Rx
Neoefrapeptin G 909093-58-9 C58H96N14O14 1R sBu H Me Me Me Me H H
Neoefrapeptin H 909093-59-0 C59H98N14O14 1R, 2R sBu H sBu H Me Me H H
Neoefrapeptin I 695200-89-6 C83H141N18O16 – sBu H sBu H Me Me iBu Rx

Neoefrapeptin L 909093-57-8 C83H141N18O16 1R, 2R, 3S, 4S sBu H sBu H sBu H iBu Rx
Neoefrapeptin M 909093-56-7 C83H141N18O16 1R, 3S, 4S sBu H Me, Me sBu H iBu Rx
Neoefrapeptin N 909093-55-6 C80H135N18O16 – Me Me Me Me Me Me iBu Ry
Et Me
Me Me
NH N+ NH N+
S S
Note: Rx, ; Ry, ; Et, –CH2CH3; iBu, –CH2CH(CH3)2; sBu, –CH(CH3)CH2CH3
O i-Bu O i-Bu N
N
175
Fig. 8.5 Basic structure of i-Pr R4 O i-Pr R3

O i-Bu O
bassianolides
N N i-Bu
O O O
i-Bu N N
O O O
R1 i-Pr O R2 i-Pr n
O i-Bu O
Table 8.5 Analogues of bassianolides

CAS Molecular
Analogues name registry no. formula n R1 R2 R3 R4
Bassianolide (9CI); NSC 321804 64763-82-2 C48H84N4O12 1 Me Me Me Me
Bassianolide,2-L-leucine-(9CI) 76646-32-7 C47H82N4O12 1 H Me Me Me
Bassianolide,2-L-leucine-4-L-leu- 76646-33-8 C46H80N4O12 1 H Me Me H
cine-(9CI)
Bassianolide,2-L-leucine-6-L-leu- 76657-98-2 C46H80N4O12 1 Me H Me H
cine-(9CI)
Bassianolide,2-L-leucine-4-L-leu- 76646-34-9 C45H78N4O12 1 H H Me H
cine-6-L-leucine-(9CI)
Cyclo(3-methyl-D-2- 76646-35-0 C44H76N4O12 1 H H H H
hydroxybutanoyl-L-leucyl-3-
methyl-D-2-hydroxybutanoyl-L-
leucyl-3-methyl-D-2-
hydroxybutanoyl-L-leucyl-3-
methyl-D-2-hydroxybutanoyl-L-
leucyl)
Bassianolide,8a-endo-(D-2- 71326-79-9 C60H105N5O15 2 Me Me Me Me
hydroxy-3-methylbutanoic acid)-
8b-endo-(N-methyl-L-leucine)-;
1,7,13,19,25-pentaoxa-
4,10,16,22,28-pentaazacyclo-
triacontane, cyclic peptide deriv.;
decabassianolide
Fig. 8.6 Basic structure of

beauvericins A–F
inhibitory activity and cytotoxicity of metastatic prostate cancer cell line PC-3M.
Beauvericins H1–H3 increased cytotoxicity without affecting antihaptotactic activ-
ity (Xu et al. 2007).
Fig. 8.7 Basic structure of

beauvericins
Table 8.6 Analogues of beauvericins A–F

name no. formula R1 R2 R3 R4 R5 R6 R7
Beauvericin A 165467-50-5 C46H59N3O9 iPr iPr iBu Me Me Me Ph
Beauvericin B 165467-51-6 C47H61N3O9 iPr iBu iBu Me Me Me Ph
Beauvericin C 444585-79-9 C48H63N3O9 iBu iBu iBu Me Me Me Ph
Beauvericin D 728912-25-2 C44H55N3O9 iPr iPr iPr H Me Me Ph
Beauvericin E 728912-26-3 C41H57N3O9 iPr iPr iPr Me H Me iBu
Beauvericin F 728912-27-4 C46H59N3O9 iBu iPr iPr iPr Me Me Ph
Note: iPr, –CH(CH3)2; Ph, –C6H5
Table 8.7 Analogues of beauvericin and beauvericins G–H

Analogues name CAS registry no. Molecular formula R1 R2 R3 R4 R5 R6
Beauvericin 26048-05-5 C45H57N3O9 Me Me Me H H H
Beauvericin G1 CID 23643017 C44H55N3O9 H Me Me H H H
Beauvericin G2 CID 23643107 C43H53N3O9 H Me Me H H H
Beauvericin G3 CID 23643015 C42H51N3O9 H H H H H H
Beauvericin H1 CID 23643108 C45H56FN3O9 Me Me Me F H H
Beauvericin H2 CID 23643016 C45H55F2N3O9 Me Me Me F F H
Beauvericin H3 CID 23643109 C45H54F3N3O9 Me Me Me F F F
Note: CID, Identity of PubChem Compound
The insecticidal effects of beauvericins at a microgram level were reported in

several insects such as Calliphora erythrocephala, Aedes aegypti, Lygus spp.,
Spodoptera frugiperda, and Schizaphis graminum (Wang and Xu 2012).
Besides being insecticidal, beauvericins have other bioactivities. Beauvericins
strongly inhibited mycelial growth of both Phytophthora sojae and Aphanomyces
cochlioides (Putri et al. 2013). They could significantly strengthen the fungicidal
activity of ketoconazole (KTC); combinations of beauvericin (0.5 mg/kg) and KTC
(0.5 mg/kg) prolonged the survival of the host infected with Candida parapsilosis
and reduced fungal colony counts in animal organs including the kidneys, lungs,
and brains. Such an effect was not achieved even with a high dose of 50 mg/kg
KTC. This supports a prospective strategy for antifungal therapy (Zhang

et al. 2007). The cytotoxicity of beauvericins on human colon adenocarcinoma
(Caco-2) cells was also reported (Prosperini et al. 2013).
Acetyl coenzyme A (acyl-CoA:cholesterol acyltransferase, ACAT) might be a
target protein of beauvericins (Tomoda et al. 1992), and ionophores produced by
beauvericins have been studied (Steinrauf 1985; Makrlik et al. 2013a, b, c, d).
Traces of beauvericins have also been detected in animal tissues (Jestoi
et al. 2007); this might be because of the fact that various Fusarium species produce
beauvericins which then enter the food chain (Moretti et al. 2007). Therefore, more
and more attention is being paid to beauvericins as a food safety risk factor
(Vaclavik et al. 2013; Juan et al. 2014; Luciano et al. 2014).
8.3.3 Beauverolides and Beauveriolides
Beauverolides were isolated from the genera Beauveria and Paecilomyces

(Elsworth and Grove 1977, 1980; Grove 1980; Mochizuki et al. 1993b; Jegorov
et al. 1994; Matsuda et al. 2004) (Fig. 8.8 and Table 8.8). They have a four-
membered cyclopeptide molecular structure containing L-phenylalanine, L-alanine,
D-leucine, and 3-hydroxy-4-methyl decylic acid with the molecular weight approx-
imately 500 Da. Beauverolide L inhibited the adhesion, extension, and phagocyto-
sis of plasmatocyte of G. mellonella (Vilcinskas et al. 1999). The target of its action
might be acetyl coenzyme A (acyl-CoA:cholesterol acyltransferase, ACAT).
Beauveriolides, having a similar structure as beauverolides, were isolated from
the culture broth of Beauveria spp. (Mochizuki et al. 1993a; Omura and Tomoda
2002; Matsuda et al. 2004) (Fig. 8.9 and Table 8.9). Beauveriolide I and
beauveriolide III show promising antiatherogenic and anti-obesity activities
(Namatame et al. 2004; Tomoda and Omura 2007). Study on its mechanism of
action revealed that beauveriolides inhibited macrophage acyl-CoA:cholesterol
acyltransferase (ACAT) to block the synthesis of cholesteryl ester (CE), leading
to a reduction of lipid droplets in macrophages (Ohshiro et al. 2009; Tomoda
et al. 2010). It was reported that beauveriolide I is a potent antiaging agent (Nakaya
et al. 2012).
Beauveriolides I and III can potently decrease Abeta secretion from cells
expressing human amyloid precursor protein; this offers a potential new scaffold
for the development of compounds with proven bioavailability for the treatment of
Alzheimer’s disease (AD) (Witter et al. 2009).
Fig. 8.8 Basic structure of R4 O H

O N R3
beauverolides
4 3
O
H
N 2
1
N O
R2 H
R1
O
8.3.4 Conoideocrellides and Paecilodepsipeptides
Conoideocrellide A and its linear derivatives, conoideocrellides B–D (Fig. 8.10 and
Table 8.10), were isolated from the scale insect pathogenic fungi Conoideocrella
tenuis BCC 18627 and Paecilomyces militaris (Isaka et al. 2011; Zhang et al. 2012).
Conoideocrellide A is structurally very similar to paecilodepsipeptide A, which was
previously isolated from T. luteorostrata BCC 9617 and its anamorph
Paecilomyces cinnamomeus BCC 9616 (Isaka et al. 2007a, b).
Paecilodepsipeptide A, a cyclohexadepsipeptide possessing three D-amino acid
residues, together with its linear analogues paecilodepsipeptides B and C (Fig. 8.11
and Table 8.11), was isolated from the insect pathogenic fungus P. cinnamomeus
BCC 9616 (Isaka et al. 2007b). The products have remarkable antiproliferative
activity on human hepatocellular carcinoma cell line (SMMC-7721) and human
lymphoma cell line (Raji), which showed good antitumor activity (Yang
et al. 2013). Paecilodepsipeptide A showed activity against the malarial parasite
Plasmodium falciparum and breast cancer MCF-7 cell lines (Isaka et al. 2007b).
8.3.5 Cordycommunin
Cordycommunin was isolated from the insect pathogenic fungus Ophiocordyceps

communis BCC 16475 (Haritakun et al. 2010). The molecular formula of
cordycommunin was established as C43H69N7O11. It contains the amino acids
valine (Val), alanine (Ala), glutamine (Gln), threonine (Thr), and tyrosine (Tyr).
Cordycommunin showed growth inhibition of Mycobacterium tuberculosis H37Ra
with an MIC value of 15 μM. This compound also exhibited weak cytotoxicity to
KB cells with an IC50 of 45 μM, while it was inactive against BC, NCI-H187, and
Vero cell lines at a concentration of 88 μM (50 μg/mL) (Haritakun et al. 2010).
Figure 8.12 shows the structure of cordycommunin.
8.3.6 Destruxins
Destruxins, a series of cyclohexadepsipeptidic mycotoxins, were first isolated from

a culture medium of entomogenous fungus Oospora destructor (later renamed as
Metarhizium anisopliae) (Kodaira 1961, 1962). They were also isolated from
Table 8.8 Analogues of beauverolides
180
CAS registry Molecular Geometry

Analogues name no. formula configuration R1 R2 R3 R4
Beauverolide A 75920-37-5 C30H47N3O5 – iPr iPr –CH2–Ph –CH(CH3)
(CH2)5CH3
Beauverolide B 75947-02-3 C31H49N3O5 – sBu –CH2–Ph iPr –CH(CH3)
(CH2)5CH3
Beauverolide Ba 13594-27-9 C31H49N3O5 – sBu –CH2–Ph iPr –CH(CH3)
(CH2)5CH3
Beauverolide C 75899-64-8 C35H49N3O5 – sBu –CH2–Ph –CH2–Ph –CH(CH3)
(CH2)5CH3
Beauverolide Ca 13594-29-1 C35H49N3O5 – sBu –CH2–Ph –CH2–Ph –CH(CH3)
(CH2)5CH3
Beauverolide D 75899-63-7 C28H43N3O5 – iPr iPr –CH2–Ph –CH(CH3)
(CH2)3CH3
Beauverolide E 75947-01-2 C29H45N3O5 1R, 2S, 3S A –CH2–Ph iPr –CH(CH3)
(CH2)3CH3
Beauverolide Ea 75899-62-6 C29H45N3O5 1R, 2S, 3S B –CH2–Ph iPr –CH(CH3)
(CH2)3CH3
Beauverolide F 75947-00-1 C33H45N3O5 – sBu –CH2–Ph –CH2–Ph –CH(CH3)
(CH2)3CH3
Beauverolide Fa (Beauveriolide IX) 75899-61-5 C33H45N3O5 – sBu –CH2–Ph –CH2–Ph –CH(CH3)
(CH2)3CH3
Beauverolide H 62995-90-8 C27H41N3O5 – iBu Me –CH2–Ph –(CH2)5CH3
Beauverolide I 62995-91-9 C29H45N3O5 – iBu Me –CH2–Ph –(CH2)7CH3
Beauverolide Ja 76265-41-3 C35H46N4O5 1R, 2S, 3S C –CH2–Ph D –CH(CH3)
(CH2)3CH3
Beauverolide Ka 76265-42-4 C37H50N4O5 – sBu –CH2–Ph D –CH(CH3)
(CH2)5CH3
Q. Hu and T. Dong
Beauverolide L 154491-56-2 C29H45N3O5 1R, 2S, 3S, 4S iBu Me –CH2–Ph E
Cyclo[L-alanyl-D-leucyl-(3S,4S)-3- 413579-49-4 C27H41N3O6 1R, 2S, 3S, 4S iBu Me –CH2–Ph– G
hydroxy-4-methyloctanoyl-L-tyrosyl] OH
(9CI)
HN
Et Me Et Me Me Et Me
(CH2)5 CH3 S
Note: nBu, –CH(CH2)2CH3A= R
; B= S
; C=
S
; D= CH2 ; E= S
; F=
Me n-Bu
181
Fig. 8.9 Basic structure of O R1

O O H
beauveriolides R3
1(R) (S)
Me 2(S) N 4
H H
N
3(S)
N
H O
R2 O
Table 8.9 Analogues of beauveriolides

CAS registry Molecular Geometry
Analogues name no. formula configuration R1 R2 R3
Beauveriolide I 154491-55-1 C27H41N3O5 1R, 2S, 3S, iBu –CH2–Ph nBu
4S, 5S
Beauveriolide III 221111-70-2 C27H41N3O5 1R, 2S, 3S, B Me Me
4S, 5S
Beauveriolide IV 460352-01-6 C22H39N3O5 1R, 2S, 3S iPr iPr nBu
Beauveriolide V 460352-02-7 C23H41N3O5 1R, 2S, 3S B iPr nBu
Beauveriolide VI 413579-48-3 C23H41N3O5 1R, 2S, 3S, iBu iPr nBu
4S, 5S
Beauveriolide VII 460352-03-8 C26H39N3O5 1R, 2S, 3S iPr –CH2–Ph nBu
Beauveriolide VII 460352-04-9 C25H45N3O5 1R, 2S, 3S B iPr –(CH2)5–
Me
R1 O
O
(R )
N 1
5 H (R)
HO O NH O
(S) 2
4 (R)
NH HN O
H
N (S)
O 3
Fig. 8.10 Conoideocrellide A and paecilodepsipeptide A
Aschersonia and other fungal species (Pedras et al. 2002). The general formula of
destruxin is cyclo(-D-HA-L-Pro-L-Ile-L-MeVal-L-MeAla-b-Ala-), where HA rep-
resents a D-α-hydroxyl acid group (Fig. 8.13 and Table 8.12). Among 39 destruxin
analogues, destruxins A, B, and E (DA, DB, and DE, respectively) show substantial
bioactivity (Liu and Tzeng 2012). However, the linear molecule resulting from the
Table 8.10 Analogues of conoideocrellides

Molecular Geometry
Analogues name CAS registry no. formula configuration R1
Conoideocrellide A 1296607-05-0 C40H47N5O10 1R, 2S, 3S, 4R, 5R OH
Paecilodepsipeptide A 931423-99-3 C40H47N5O9 1R, 2S, 3S, 4R, 5R H
O (E)
R3
R1 O
1
2 (R)
N CO2R2
(S) H
HO O NH HO
(S) 5
(R)
NH HN O
3
H
N (S)
O 4
O
Fig. 8.11 Conoideocrellides B–D and paecilodepsipeptides B–C
Table 8.11 Analogues of paecilodepsipeptides

CAS Molecular Geometry
Analogues name registry no. formula configuration R1 R2 R3
Conoideocrellide B 1296607-06-1 C40H49N5O10 1R, 2S, 3S, 4R, 5S OH H H
Conoideocrellide C 1296607-07-2 C41H51N5O10 1R, 2S, 3S, 4R, 5S OH Me H
Conoideocrellide D 1296607-08-3 C41H51N5O12 1R, 2S, 3S, 4R, 5S OH Me OH
Paecilodepsipeptide B 931423-29-9 C40H49N5O10 1R, 2S, 3S, 4R, 5S H H H
Paecilodepsipeptide C 931423-31-3 C41H51N5O10 1R, 2S, 3S, 4R, 5S H Me H
opening of the DA cycle is not toxic, and DE would degrade to less toxic DE-diol
upon enzymatic action (Jegorov et al. 1992; Dumas et al. 1994).
Destruxins have insecticidal activity against many pests with various modes of
action. Destruxins via hemocoel injection exhibited promising insecticidal activity
in early studies. Further researches discovered that destruxins showed contact
action (Hu et al. 2007b), gut toxicity (Brousseau et al. 1996), antifeedant effect
(Amiri-Besheli et al. 2000, Hu et al. 2007a), and ovicidal and oviposition-deterrent
activity (Pedras et al. 2002). The mixture of destruxins and Bt, Paecilomyces, and
Fig. 8.12 Structure of O NH2

cordycommunin
H H
O N N
(S)
N (S)
H (S)
(S) O O
NH NH OH
O
H
N (S)
O
O
(R)
(R) O
O
HO
Fig. 8.13 Chemical R6 O

H
structure of destruxins N O O
O
H N
R5 H
N n
N N O
O O
R4
R3 R2 R1
botanical insecticides had synergistic effect (Hu et al. 2007b; Rizwan-Ul-Haq

et al. 2009; Yi et al. 2012).
Destruxins damage the innate immunity of insects. Morphology and function of
encapsulation and phagocytosis processes of insect hemocytes were destroyed by
destruxins (Vilcinskas et al. 1997a, b; Vey et al. 2002; Fan et al. 2013). The
expression of antimicrobial peptides was also reduced in Drosophila melanogaster
treated with DA (Pal et al. 2007). Destruxin also acts as a kind of calcium ionophore
and an inhibitor of V-H+-ATPase (Bandani et al. 2001; Chen et al. 2014; Vazquez
et al. 2005). Other studies have reported that destruxins damage the insect organs
and tissues, including the midgut, Malpighian tubules, salivary glands, and muscles
(Kershaw et al. 1999; Ruiz-Sanchez et al. 2010; Ruiz-Sanchez and O’Donnell 2012;
Sree and Padmaja 2008). However, the molecular mechanism of damage caused by
destruxins has not yet been determined.
Destruxins were well reviewed (Pedras et al. 2002; Liu and Tzeng 2012), the
authors do not repeat here.
8.3.7 Enniatins
Enniatin was first discovered in the 1940s (Gaumann et al. 1947). Enniatin ana-
logues were produced by various species of the fungal genera Verticillium and
Fusarium, etc. (Herrmann et al. 1996; Supothina et al. 2004) (Fig. 8.14 and
Table 8.13). They are N-methylated cyclohexadepsipeptides, composed of three
Table 8.12 Analogues of destruxins

Destruxin analogues n R1 R2 R3 R4 R5 R6
Destruxin A 1 CH(Me) Me H Me Me CH¼CH2
CH2Me
Destruxin A1 2 CH(Me) Me H Me Me CH¼CH2
CH2Me
Destruxin A2 1 CHMe2 Me H Me Me CH¼CH2
Destruxin A3 0 CH(Me) Me H Me Me CH¼CH2
CH2Me
Destruxin A4 1 CH(Me) Me Me Me Me CH¼CH2
CH2Me
Destruxin A5 1 CH(Me) Me Me Me Me CH¼CH2
CH2Me
Destruxin A4 chlorohydrin 1 CH(Me) Me Me Me Me CHOHCH2Cl
CH2Me
Desmethyl destruxin A 1 CH(Me) H H Me Me CH¼CH2
CH2Me
Dihydro-destruxin A 1 CH(Me) Me H Me Me CH2Me
CH2Me
Pseudo-destruxin A 1 CH(Me) Me H Me Me CHMe2
CH2Me
Destruxin B 1 CH(Me) Me H Me Me CHMe2
CH2Me
Destruxin B1 2 CH(Me) Me H Me Me CHMe2
CH2Me
Destruxin B2 1 CHMe2 Me H Me Me CHMe2
Desmethyl destruxin B 1 CH(Me) Me H Me Me CHMe2
CH2Me
Desmethyl destruxin B2 1 CHMe2 Me H Me Me CHMe2
Homo-destruxin B 1 CH(Me) Me Me Me Me CHMe2
CH2Me
Proto-destruxin B 1 CH(Me) Me H H Me CHMe2
CH2Me
Hydroxy destruxin B 1 CH(Me) Me H Me Me CHMe2
CH2Me
Hydroxyhomo-destruxin B 1 CH(Me) Me Me Me Me CHMe2
CH2Me
β-D-Glucopyranosyl- 1 CH(Me) Me H Me Me
hydroxyl destruxin B CH2Me
[Phe3, N-Me-Val5] 1 CH2Ph Me H Me CHMe2 CHMe2
Destruxin B
Pseudo-destruxin B 1 CH2Ph Me H Me CH2CHMe2 CHMe2
Destruxin C 1 CH(Me) Me H Me Me CHMeCH2OH
CH2Me
Destruxin C2 1 CHMe2 Me H Me Me CHMeCH2OH
Desmethyl destruxin C 1 CH(Me) H H Me Me CHMeCH2OH
CH2Me
(continued)
Table 8.12 (continued)

Destruxin analogues n R1 R2 R3 R4 R5 R6
Pseudo-destruxin C 1 CH2Ph Me H Me CHMe2 CHMeCH2OH
Destruxin D 1 CH(Me) Me H Me Me CHMeCOOH
CH2Me
Destruxin D1 2 CH(Me) Me H Me Me CHMeCOOH
CH2Me
Destruxin D2 1 CHMe2 Me H Me Me CHMeCOOH
Destruxin E 1 CH(Me) Me H Me Me Oxirane
CH2Me
Destruxin E1 2 CH(Me) Me H Me Me Oxirane
CH2Me
Destruxin E2 1 CHMe2 Me H Me Me Oxirane
Destruxin E chlorohydrin 1 CH(Me) Me H Me Me CHOHCH2Cl
CH2Me
Destruxin E2 chlorohydrin 1 CHMe2 Me H Me Me CHOHCH2Cl
Destruxin E diol 1 CH(Me) Me H Me Me CHOHCH2OH
CH2Me
Destruxin Ed1 2 CH(Me) H H Me Me CHOHCH2OH
CH2Me
[β-Me-Pro] Destruxin E 1 CH(Me) Me H Me Me CHOHCH2Cl
chlorohydrin CH2Me
Regioisomer of destruxin E 1 CH(Me) Me H Me Me CHClCH2OH
chlorohydrin CH2Me
Destruxin F 1 CH(Me) Me H Me Me CHOHMe
CH2Me
Notes: In destruxin A3 and destruxin A5, the proline residue is replaced by and
, respectively. The destruxin E chlorohydrin and [β-Me-Pro] destruxin E chlorohydrin
share the same empirical formula. They are stereoisomers at R6
units each of N-methylated branched-chain L-amino acid and D-2-hydroxy acid

arranged in an alternate fashion (Firakova et al. 2007).
Enniatins have been shown to act against spruce budworm larvae
(Choristoneura fumiferana) (Strongman et al. 1988), G. mellonella (Mule
et al. 1992), and S. frugiperda cell line (Sf-9) (Fornelli et al. 2004). Enniatins
have multi-activities including antifungal, antibiotic, and cytotoxic properties. To
date, 29 enniatins have been isolated and characterized, either as a single compound
or mixtures of inseparable analogues. Fusafungine, a drug developed from a
mixture of enniatins, is used as a topical treatment of upper respiratory tract
infections by oral and/or nasal inhalation (Sy-Cordero et al. 2012).
In regard to the molecular mechanism of enniatins, researches indicated that they
inhibit ABC transporters (Hiraga et al. 2005), suppress acyl-CoA:cholesterol
Fig. 8.14 Basic structure of R1 O R4

enniatins R2 R3 O
O O
1 N 3 N
2
5 O
O 4
6 N
O
O R6
R8 R7 O R5
acyltransferase (ACAT) (Tomoda et al. 1992), and act as ionophores (Levy

et al. 1995; Doebler 2000).
Enniatins are also a common contaminant in grain-based foods, so, research on
their harmful effects on human health is gaining more importance (Feudjio
et al. 2010; Santini et al. 2012).
8.3.8 Hirsutellides and Hirsutides
Hirsutellide A, a cyclohexadepsipeptide, was first isolated from a cell extract of the

entomogenous fungus Hirsutella kobayasii BCC 1660 (Vongvanich et al. 2002)
(Fig. 8.15). Hirsutellide A exhibited antimycobacterial and antimalarial activities,
but was inactive toward the Vero cell line (at 50 μg/mL) (Vongvanich et al. 2002).
An entomogenous fungus Hirsutella spp., isolated from an infected spider, was
found to produce the cyclotetrapeptide hirsutide, with the amino acid sequence of
cyclo-(L-NMe-Phe-L-Phe-L-NMe-Phe-L-Val) (Lang et al. 2005) (Fig. 8.16). No
other hirsutellide and hirsutide analogues have been reported yet.
8.3.9 Isariins, Isaridins, and Isarolides
Isariin was first isolated from Isaria cretacea (Vining and Taber 1962). Then, its
analogues were isolated and structurally elucidated (Baute et al. 1981; Deffieux
et al. 1981; Langenfeld et al. 2011). Isariins possess a β-hydroxyl fatty acid and five
α-amino acid residues (Fig. 8.17 and Table 8.14). Isariins were originally shown to
have insecticidal activity against G. mellonella (Baute et al. 1981). Isariins have
been reported to have an inhibitory effect on the intraerythrocytic growth of
Plasmodium falciparum (Sabareesh et al. 2007). Iso-isariin B was found to be
active against the pest insects Sitophilus spp. with an LC50 value of 10 μg/mL
(Langenfeld et al. 2011).
Isaridins A and B were first isolated from Isaria (Ravindra et al. 2004), and then,
other analogues were isolated and structurally elucidated (Sabareesh et al. 2007). In
contrast to isariin, they have an α-hydroxyl acid and a β-amino acid, with a
preponderance of N-alkylated residues (Fig. 8.18 and Table 8.15). Isaridin also
Table 8.13 Analogues of enniatins
188
Analogues name CAS registry no. Molecular formula Geometry configuration R1 R2 R3 R4 R5 R6 R7 R8

Enniatin A 2503-13-1 C36H63N3O9 1S, 2R, 3S, 4R, 5S, 6R Rx Me iPr Rz iPr Rz Me iPr
Enniatin A1 4530-21-6 C35H61N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr Rz iPr Rz Me iPr
Enniatin B 917-13-5 C33H57N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr iPr iPr iPr Me iPr
Enniatin B1 19914-20-6 C34H59N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr Rz iPr iPr Me iPr
Enniatin B2 632-91-7 C32H55N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr iPr iPr iPr H iPr
Enniatin B3 864-99-3 C31H53N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr H iPr iPr iPr iPr H iPr
Enniatin B5 1338161-36-6
Enniatin C 19893-23-3 C36H63N3O9 1S, 2R, 3S, 4R, 5S, 6R iBu Me iPr iBu iPr iBu Me iPr
Enniatin D(B4) 19893-21-1 C34H59N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr iBu iPr iPr Me iPr
Enniatin E1 1450880-97-3 C35H61N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr iPr Ro iPr Me Rz
Enniatin E2 1450880-98-4 C35H61N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr Rz iPr iBu Me iPr
Enniatin F(A2) 144446-20-8 C36H63N3O9 – iBu Me iPr Rz iPr Rz Me iPr
Enniatin G 19893-22-2 C35H61N3O9 – iBu Me iPr iBu iPr iPr Me iPr
Enniatin H 561298-15-5 C34H59N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me Rz iPr iPr iPr Me iPr
Enniatin I 561298-16-6 C35H61N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me Rz iPr iPr iPr Me Rz
Enniatin J1 19893-15-3 C31H53N3O9 1S, 2R, 3S, 4R, 5S, 6R Me Me iPr iPr iPr iPr Me iPr
Enniatin J2 716318-01-3 C32H55N3O9 1S, 2R, 3S, 4R, 5S, 6R Me Me iPr Rz iPr iPr Me iPr
Enniatin J3 716318-02-4 C32H55N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr Rz iPr Me Me iPr
Enniatin K1 716318-00-2 C32H55N3O9 1S, 2R, 3S, 4R, 5S, 6R Et Me iPr iPr iPr iPr Me iPr
Enniatin L 791785-16-5 C32H55N3O10 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr iPr Ro iPr Me iPr
Enniatin MK1688 133869-46-2 C36H63N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me Rz iPr Rz iPr Me Rz
Enniatin M1 791785-17-6 C35H61N3O10 1S, 2R, 3S, 4R, 5S, 6R iPr Me iPr iPr Ro iPr Me Rz
Enniatin M2 791785-18-7 C35H61N3O10 1S, 2R, 3S, 4R, 5S, 6R iPr Me Rz iPr Ro iPr Me iPr
Enniatin N 791785-19-8 C36H63N3O10 1S, 2R, 3S, 4R, 5S, 6R iPr Me Rz iPr Ro iPr Me Rz
Enniatin O1 847371-30-6 C35H61N3O9 1S, 2R, 3S, 4R, 5S, 6R iBu Me Rz iPr iPr iPr Me iPr
Q. Hu and T. Dong
Enniatin O2 847371-31-7 C35H61N3O9 1S, 2R, 3S, 4R, 5S, 6R iBu Me iPr iPr Rz iPr Me iPr
Enniatin O3 847371-32-8 C35H61N3O9 1S, 2R, 3S, 4R, 5S, 6R iPr Me Rz iBu iPr iPr Me iPr
Enniatin P1 1172635-53-8 C32H55N3O10 1S, 2S, 3S, 4S, 5S, 6S iPr Me iPr Rp iPr iPr Me iPr
Enniatin P2 1172635-54-9 C33H57N3O10 1S, 2S, 3S, 4S, 5S, 6S Rp Me iPr iBu iPr iPr Me iPr
Et
Me R
Note: Rz, S Et
; Ro, ; Rp,
OH OH
189
Fig. 8.15 Structure of

hirsutellide A
O O
O
H
(R) N
(R)
N O
(S)
O (S)
N
(R) N
H
O (R)
O O
Fig. 8.16 Structure of

hirsutide
O H H
N O
(S)
N (S)
H
(S) N H
N
H H (S) O
O
Fig. 8.17 Basic structure of R2 O

H
isariins O N
O 5(R) Pr-i
N
H 4 (S)
O H
N 3(R)
1(S) 2(S)
O
R1
N O i-Bu N
H
H
Me O
showed an inhibitory effect on the intraerythrocytic growth of Plasmodium

falciparum (Sabareesh et al. 2007).
Isarolides A, B, and C were isolated from a new species of Isaria. Their
structures have been elucidated (Briggs et al. 1966) (Fig. 8.19 and Table 8.16).
Isarolide A is identical to beauverolide Ba, while isarolide C is identical with
beauverolide Ca (Elsworth and Grove 1980).
Table 8.14 Analogues of isariins

name no. formula configuration R1 R2
Isariin A 10409-85-5 C33H59N5O7 – iPr –(CH2)8–Me
Isariin B 80111-95-1 C30H53N5O7 1S, 2S, 3R, 4S, 5R iPr –(CH2)5–Me
Isariin C 80111-96-2 C28H49N5O7 1S, 2S, 3R, 4S, 5R Me –(CH2)5–Me
Isariin C2 944347-21-1 C28H49N5O7 1S, 2S, 3R, 4S, 5R iPr nBu
Isariin D 80111-97-3 C26H45N5O7 1S, 2S, 3R, 4S, 5R Me nBu
Isariin E 944347-11-5 C27H45N5O7 1S, 2S, 3R, 4S, 5R iPr nPr
Isariin F2 944347-18-6 C29H51N5O7 1S, 2S, 3R, 4S, 5R iPr –(CH2)4–Me
Isariin G1 944347-20-0 C31H55N5O7 1S, 2S, 3R, 4S, 5R iPr –(CH2)8–Me
Isariin G2 944347-19-7 C31H55N5O7 1S, 2S, 3R, 4S, 5R Me –(CH2)6–Me
Iso-isariin B 1290627-99-4 C30H53N5O7 1S, 2S, 3S, 4S iPr –CH(CH2)3(CH3)2
Note: nPr, –CH2CH2CH3
Fig. 8.18 Basic structure O H O

N O i-Bu
of isaridins O
5(S) N
R1 1(S) i-Pr H
H 4
2(S) N (S)
3(S)
N N O
R2
O H
Me O
Me
Ph
Table 8.15 Analogues of isaridins

Analogues Molecular Geometry
name CAS registry no. formula configuration R1 R2
Isaridin A 780781-87-5 C39H53N5O7 1S, 2S, 3S, 4S, 5S Ph H
Isaridin B 780781-88-6 C40H55N5O7 1S, 2S, 3S, 4S, 5S Ph H
Isaridin C 342573-58-4 C36H55N5O7 1S, 2S, 3S, 4S, 5S iBu H
Isaridin C1 944346-45-6
Isaridin C2 943896-54-6 C36H55N5O7 1S, 2S, 3S, 4S, 5S iPr Me
Isaridin D 944346-46-7
Isaridin E 944347-22-2 C35H53N5O7 1S, 2S, 3S, 4S, 5S iPr H
Fig. 8.19 Basic structure R1 H

O O N R2
of isarolide
O H
N
N O
H
O R3
Table 8.16 Analogues of isarolides

name no. formula configuration R1 R2 R3
Isarolide A 13594-27-9 C31H49N3O5 – –CH(Me) iPr –CH2Ph
(CH2)5–Me
Isarolide B 13594-28-0 C31H49N3O5 – –(CH2)7–Me –CH2Ph iPr
Isarolide C 13594-29-1 C35H49N3O5 – –CH(Me) –CH2Ph –CH2Ph
(CH2)5–Me
Note: iPr, –CH(CH3)2; Ph, –C6H5; Me, –CH3; –, no geometry configuration
8.3.10 Serinocyclins
Serinocyclins A and B were isolated from the conidia of the entomopathogenic

fungus M. anisopliae (Krasnoff et al. 2007). The molecular structure of serinocyclin
A is cyclo-(Acc-Hyp-Ser-HyLys-β-Ala-Ser-Ser), where Acc is
1-aminocyclopropane-1-carboxylic acid, Hyp is hydroxyproline, Ser is serine,
HyLys is hydroxylysine, and β-Ala is β-alanine. Acc and HyLys are unusual
nonprotein amino acids. Serinocyclin B contains lysine (Lys) but not hydroxylysine
(HyLys). The absence of Me groups is unusual among fungal peptides and, along
with the charged lysyl side chain and multiple hydroxyl groups, contributes to the
polar nature of the compounds. Serinocyclin A produced a sublethal locomotory
defect in mosquito larvae at an EC50 of 59 ppm (Krasnoff et al. 2007). Figure 8.20
and Table 8.17 show the structures of serinocyclins.
8.3.11 Verticilide
Verticilide was first isolated from the fungal strain Verticillium spp. FKI-1033
(Omura et al. 2004). It is a 24-membered ring cyclic depsipeptide with a sequence
of cyclo[(2R)-2-hydroxyheptanoyl-N-methyl-L-alanyl] (Omura et al. 2004; Monma
et al. 2006). Another soil fungal strain, Verticillium spp. FKI-2679, was found to
produce inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT) in a cell-based
assay using ACAT1- and ACAT2-expressing CHO cells. Three new verticilide
analogues, verticilides A2, A3, and B1, were isolated from the fermentation broth.
Their chemical structures have been elucidated (Figs. 8.21 and 8.22, Table 8.18).
Verticilides A1, A2, A3, and B1 showed a high degree of selectivity toward
ACAT2, with IC50s 8.5–11-fold more potency than that observed against ACAT1
(Ohshiro et al. 2012).
Verticilides inhibited ryanodine binding to ryanodine receptors in the cockroach
at an IC50 value of 4.2 μM, whereas inhibition against mouse ryanodine receptors
was weak with an IC50 value of 53.9 μM (Monma et al. 2006; Shiomi et al. 2010).
HO
(R) OH
O
O O H
N N NH2
3(S) (S)
HN 4(S) N 1
H 2 (R)
H
N 6 (R) H
5(S) R
O N N O
H H
O
O OH
OH
Fig. 8.20 Basic structure of serinocyclins
Table 8.17 Analogues of serinocyclins

Analogues name CAS registry no. Molecular formula Geometry configuration R
Serinocyclin A 1001897-42-2 C27H44N8O12 1S, 2R, 3S, 4S, 5S, 6R OH
Serinocyclin B 1001897-43-3 C27H44N8O11 2R, 3S, 4S, 5S, 6R H
Fig. 8.21 Basic structure O

O
R1 Me
of verticilides A1–A3 Me O (CH2)4
O
1 O
2 4
O N N
3 (R)
O 6 O
8 7 N 5
O N Me
O O
Me
(CH2)4 O R2 (CH2)4
Fig. 8.22 Structure O O

Me
of verticilide B1 O (CH2)4
(H2C)4 O
N N
O O
N Me
Me O
O (CH2) 4 O
8.4 Cyclopeptides
8.4.1 Cyclosporines
Cyclosporines, or cyclosporins, discovered in the 1970s, were a series of cyclo-

undecapeptide secreted by Trichoderma polysporum and Cylindrocarpon lucidum
(Borel et al. 1976; Dreyfuss et al. 1976) (Fig. 8.23 and Table 8.19). Certain species
Table 8.18 Analogues of verticilides

name no. formula configuration R1 R2
Verticilide A1 693778-57-3 C44H76N4O12 1R, 2S, 3R, 4S, 5R, –(CH2)4–Me Me
6S, 7R, 8S
Verticilide A2 1380602-01-6 C43H74N4O12 1R, 2S, 3R, 4S, 5R, –(CH2)4–Me H
6S, 7R, 8S
Verticilide A3 1380601-94-4 C46H80N4O12 1R, 2S, 3R, 4S, 5R, –(CH2)6–Me Me
6S, 7R, 8S
Verticilide B1 1380750-89-9 C33H57N3O9 – – –
of Beauveria, Verticillium, and Tolypocladium could also produce cyclosporines

(Jegorov et al. 1990).
Cyclosporines have insecticidal activities. It was reported that cyclosporines are
effective against mosquito larvae (Weiser and Matha 1988; Podsiadlowski
et al. 1998). Cyclosporin A (CsA) has the immunosuppressive effect on insect
humoral immune response (Fiolka 2008) and cellular immune response (Vilcinskas
et al. 1999). CsA is an important immunosuppressant used for human organic
transplantation and other disease treatments (Muellenhoff and Koo 2012; Kovarik
2013). In regard to the mechanism of action as immunosuppressant, CsA specifi-
cally binds to the receptor, cyclophilin A (CypA), which is located in the cytoplasm
of T lymphocytes, and consequently inhibits CypA-mediated immune signaling
pathways (Ryffel et al. 1980; Wiesinger and Borel 1980; Kallen et al. 1991; Liu
et al. 1991).
8.4.2 Cordyheptapeptide
Cordyheptapeptide A was first isolated from the entomogenous fungal strain

Cordyceps spp. BCC 1788. Its amino acid sequence was determined as cyclo-
(D-NMePhe-L-Leu-L-Ile-L-NMeTyr-L-Phe-NMeGly-L-Pro) (Rukachaisirikul
et al. 2006). Then, the analogues, cordyheptapeptides C–E, were isolated from
the marine-derived fungus Acremonium persicinum SCSIO 115 (Chen et al. 2012)
(Fig. 8.24 and Table 8.20).
Cordyheptapeptide A exhibited antimalarial activity against Plasmodium
falciparum K1 and cytotoxicity to Vero cell lines with IC50 values of 5.35 and
>56.88 μM, respectively (Rukachaisirikul et al. 2006). The cytotoxicities of
cordyheptapeptides C–E were evaluated using human glioblastoma (SF-268),
human breast cancer (MCF-7), and human lung cancer (NCI-H460) cell lines
(Chen et al. 2012). Cordyheptapeptide E demonstrated cytotoxicity against all
three cell lines, with IC50 values of 3.2, 2.7, and 4.5 μM, respectively. Cordyhep-
tapeptide C was found to possess cytotoxicity against SF-268 and MCF-7 cells with
IC50 values of 3.7 and 3.0 μM, respectively, and weaker cytotoxicity against the
R4
O O i-Bu O
R3
H O N i-Bu O N i-Pr R5
N 9
N 7 N N
6 H 8 10
O H
N 4 N N 1
5 3 2
N N R1
i-Pr H O O
i-Bu O i-Bu O R2 O
Fig. 8.23 Basic structure of cyclosporines
NCI-H460 cell line. The most polar compound, cordyheptapeptide D, displayed no

activity against all three cell lines (Chen et al. 2012).
Cordyheptapeptides A and B were reported to possess cytotoxicities against KB
(oral human epidermoid carcinoma), BC (human breast cancer), NCI-H187 (human
small cell lung cancer), and Vero (African green monkey kidney fibroblasts) cell
lines with IC50 values of 0.78, 0.20, 0.18, and 14 μM and 2.0, 0.66, 3.1, and 1.6 μM,
respectively (Isaka et al. 2007c).
8.5 Prospects of Entomogenous Fungal NRPs
8.5.1 Prospect of NRPs for Biocontrol of Lepidopteran Pests
The insect order Lepidoptera includes very important crop pests. Use of chemical
insecticides is the main method for the control of these insects. Meanwhile,
chemical insecticides have been discouraged because of their side effects. More
and more attention is being paid to entomogenous fungi for biocontrol of these
pests. As an important pathogenic factor, NRPs will be considered more by
researchers and users. Many reports have shown that NRPs can kill lepidopteran
pest species, although NRPs have not been applied in fields yet. It is a rational
prediction that NRPs will contribute more to pest control in the near future because
of the technical development and advancement.
Advancement in technology makes the discovery of NRPs more easy and rapid.
Since enniatins were discovered in the 1940s, destruxins and beauvericins in the
1960s, beauverolides, bassianolides, cyclosporins, and efrapeptins in the 1970s,
etc., novel NRPs such as neoefrapeptins, cordyheptapeptides, verticilides,
serinocyclins, isaridins, hirsutellides, hirsutides, cordycommunin, conoideo-
crellides, and paecilodepsipeptides were successively found in this century. Mean-
while, large numbers of new analogues of NRPs were isolated and identified, for
example, since destruxin A was discovered in 1961, more than 30 kinds of ana-
logues have been successively found in the last 50 years. Because of the abundant
diversity of entomogenous fungi and their NRPSs, along with more and more new
fungal strains (e.g., endophytic and ocean species) being used as research materials,
in addition to the application of new sensitive technologies of separation and
196
Table 8.19 Analogues of cyclosporines

name no. formula Geometry configuration R1 R2 R3 R4 R5
Cyclosporin A 59865-13-3 C62H111N11O12 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10S Rx Et Me Me Me
Cyclosporin B 63775-95-1 C61H109N11O12 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10S Rx Me Me Me Me
Cyclosporin C 59787-61-0 C62H111N11O13 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10S Rx CH(OH) Me Me Me
CH3
Cyclosporin D 63775-96-2 C63H113N11O12 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10S Rx iPr Me Me Me
Cyclosporin E 63798-73-2 C61H109N11O12 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10S Rx Et Me H Me
Cyclosporin F 83574-28-1 C62H111N11O11 – –CH2CH(CH3) Et Me Me Me
CH2CH¼CHCH3
Cyclosporin G 74436-00-3 C63H113N11O12 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10S Rx nPr Me Me Me
Cyclosporin H 83602-39-5 C62H111N11O12 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10R Rx Et Me Me Me
Cyclosporin I 83563-93-3 C62H111N11O12 – –CH(OH)CH(CH3) iPr H Me Me
CH2CH¼CHCH3
Cyclosporin J 121604-28-2 C59H107N11O11 1S, 2S, 3S, 4S, 5S, 6S, 7R, 8S, 9S, 10S iBu Et Me Me Me
Cyclosporin K 108027-38-9 C63H113N11O11 – –CH2CH(CH3) iPr Me Me Me
CH2CH¼CHCH3
Cyclosporin L 108027-39-0 C61H109N11O12 – –CH(OH)CH(CH3) Et Me Me H
CH2CH¼CHCH3
Note: nPr, –CH2CH2CH3
Q. Hu and T. Dong
Fig. 8.24 Basic structure of R1

cordyheptapeptide
O
H H O
N O N
R2 1 (S) N N
2 (S) 6 (S)
3 (S) N
5(S)
O
4
N N O
H (R) O
O
R3
Table 8.20 Analogues of cordyheptapeptide

Molecular Geometry
Analogues name CAS registry no. formula configuration R1 R2 R3
Cordyheptapeptide A 877776-11-9 C49H65N7O8 1S, 2S, 3S, 4R, OH sBu H
5S, 6S
Cordyheptapeptide B 957768-12-6 C49H65N7O7 1S, 2S, 3S, 4R, H sBu H
5S, 6S
Cordyheptapeptide C 1377420-73-9 C48H63N7O8 1S, 2S, 3S, 4R, OH H H
5S, 6S
Cordyheptapeptide D 1377420-75-1 C48H63N7O9 1S, 2S, 3S, 4R, OH OH H
5S, 6S
Cordyheptapeptide E 1377420-77-3 C49H66N7O9 1S, 2S, 3S, 4R, OH OH Me
5S, 6S
detection of chemical compounds, it is a rational prediction that more novel NRPs

will be discovered at an easier and faster pace.
8.5.2 Molecular Mechanism of NRPs
Entomogenous fungi invade host insects mainly from the cuticle and must break
through the hemolymph immunity barrier after entering the insect hemocoel to
cause mycosis in insects. NRPs are the evolutionary products of fungi in order to
adapt to their host insects; therefore, NRPs play a very important role in pathoge-
nicity. Many NRPs such as destruxin and beauvericin inhibit insect immunity, some
NRPs have other toxic effects, but the mechanisms of action of NRPs are not clear
except for that of cyclosporins.
Slow insecticidal efficacy limits myco-insecticides to a small-scale application.
In order to solve the problem, it is necessary that fundamental research on the
pathogenesis of entomogenous fungi should be paid more attention. Pathogenic
process of entomogenous fungi generally includes a lot of steps: conidial attach-
ment to the insect cuticle, germination of conidium, formation of appressoria and
invading structure, penetration of the cuticle, entering the hemocoel, overcoming

insect’s innate immunity, capturing nutrition, mass proliferation, etc. Previous
studies mainly focus on the processes before fungus enters into the hemocoel
(i.e., from fungal attachment to insect surface to penetrating the cuticle).
Researchers paid less attention to how fungi can break through the host insect’s
immunity barrier after entering the hemocoel. Once the fundamental problems such
as how entomogenous fungi secrete NRPs in hemocoel and how NRPs act are
elucidated, it will provide a new direction to improve the efficacy of myco-
insecticides.
8.5.3 NRPs as Novel Immunosuppressant Insecticides
Inventing a new pesticide is an important measure of effectively controlling

agricultural pests and of developing the pesticide industry. Generally, discovery
of new pesticide includes a variety of ways such as random screening, analogue
synthesis, bioactive natural product model, and biorational design. Among them,
bioactive natural product model is the most important. In the system of bioactive
natural product model, researchers find new active natural products and optimize
their structures to get lead compounds, then they determine the new target of action
(target enzyme or receptor) of the leads, and finally, a new pesticide is discovered
by means of computer-aided molecular design, oriented synthesis, and high-
throughput screening.
Most of the insecticides in the current market act on the insect’s nervous system.
Their main target proteins include acetylcholinesterase (organophosphorus), ace-
tylcholine receptor (carbamates, neonicotinoids), sodium ion channel (pyrethroids),
γ-aminobutyric acid receptor (abamectin), etc. Numerous insecticides having the
same target protein lead to the development of pesticide resistance in insects.
8.6 Conclusion
Insect immune system is expected to become a significant and a potential target of

the new-generation pesticides. The first reason is that the immune system has
different structure and function from the nervous system, muscular system, respi-
ratory system, and endocrine system. So, the rationale is that there will be no cross
resistance between immunosuppressants and the existing pesticides. The second
reason is that the insect immune system is different from that of mammals. It will
benefit in developing highly selective immunosuppressant insecticides. A large
number of NRPs have been reported to have natural immunosuppressant properties,
and new pesticide targets could be found via further study which will provide new
insights into future research and development of new immunosuppressant
insecticides.
Acknowledgements The authors cordially thank Dr. Zhou Zuoqiang and his MS student Liu Kai
(College of Science, South China Agricultural University) for checking the chemical structures of
NRPs. Ms. Chen Xiurun partly contributes to this MS. This research is supported by the National
High-Technology Research and Development Program (“863” Program) of China
(2011AA10A204-2).
References
Amiri-Besheli B, Khambay B, Cameron S, Deadman ML, Butt TM (2000) Inter- and intra-specific
variation in destruxin production by insect pathogenic Metarhizium spp., and its significance to
pathogenesis. Mycol Res 104:447–452. doi:10.1017/s095375629900146x
Bandani AR (2004) Effect of entomopathogenic fungus Tolypocladium species metabolite
efrapeptin on Galleria mellonella agglutinin. Commun Agric Appl Biol Sci 69:165–169
Bandani AR, Amiri B, Butt TM, Gordon-Weeks R (2001) Effects of efrapeptin and destruxin,
metabolites of entomogenous fungi, on the hydrolytic activity of a vacuolar type ATPase
identified on the brush border membrane vesicles of Galleria mellonella midgut and on plant
membrane bound hydrolytic enzymes. Biochim Biophys Acta Biomembr 1510:367–377.
doi:10.1016/s0005-2736(00)00370-9
Baute R, Deffieux G, Merlet D, Baute MA, Neveu A (1981) New insecticidal cyclodepsipeptides
from the fungus Isaria felina. I. Production, isolation and insecticidal properties of isariins B, C
and D. J Antibiot 34:1261–1265. doi:10.7164/antibiotics.34.1261
Bernardini M, Carilli A, Pacioni G, Santurbano B (1975) Isolation of beauvericin from
Paecilomyces fumosoroseus. Phytochemistry 14:1865. doi:10.1016/0031-9422(75)85311-8
Boettger D, Hertweck C (2013) Molecular diversity sculpted by fungal PKS-NRPS hybrids.
Chembiochem 14:28–42. doi:10.1002/cbic.201200624
Boot CM, Amagata T, Tenney K, Compton JE, Pietraszkiewicz H, Valeriote FA, Crews P (2007)
Four classes of structurally unusual peptides from two marine-derived fungi: structures and
bioactivities. Tetrahedron 63:9903–9914. doi:10.1016/j.tet.2007.06.034
Borel JF, Feurer C, Gubler HU, Staehelin H (1976) Biological effects of cyclosporin A: a new
antilymphocytic agent. Agents Actions 6:468–475. doi:10.1007/bf01973261
Briggs LH, Fergus BJ, Shannon JS (1966) Chemistry of fungi. IV. Cyclodepsipeptides from a new
species of Isaria. Tetrahedron 22:269–278
Brousseau C, Charpentier G, Belloncik S (1996) Susceptibility of spruce budworm, Choristoneura
fumiferana Clemens, to destruxins, cyclodepsipeptidic mycotoxins of Metarhizium anisopliae.
J Invertebr Pathol 68:180–182. doi:10.1006/jipa.1996.0079
Chen Z, Song Y, Chen Y, Huang H, Zhang W, Ju J (2012) Cyclic heptapeptides, cordyhepta-
peptides C-E, from the marine-derived fungus Acremonium persicinum SCSIO 115 and their
cytotoxic activities. J Nat Prod 75:1215–1219. doi:10.1021/np300152d
Chen XR, Hu QB, Yu XQ, Ren SX (2014) Effects of destruxins on free calcium and hydrogen ions
in insect hemocytes. Insect Sci 2:31–38
De Zotti M, Biondi B, Crisma M, Hjorringgaard CU, Berg A, Bruckner H, Toniolo C (2012)
Isovaline in naturally occurring peptides: a nondestructive methodology for configurational
assignment. Biopolymers 98:36–49. doi:10.1002/bip.21679
Deffieux G, Merlet D, Baute R, Bourgeois G, Baute MA, Neveu A (1981) New insecticidal
cyclodepsipeptides from the fungus Isaria felina. II. Structure elucidation of isariins B, C
and D. J Antibiot 34:1266–1270. doi:10.7164/antibiotics.34.1266
Doebler JA (2000) Effects of neutral ionophores on membrane electrical characteristics of NG108-
15 cells. Toxicol Lett 114:27–38. doi:10.1016/s0378-4274(99)00193-9
Dreyfuss M, Haerri E, Hofmann H, Kobel H, Pache W, Tscherter H (1976) Cyclosporin A and

C. New metabolites from Trichoderma polysporum (Link ex Pers.) Rifai. Eur J Appl Microbiol
3:125–133
Dumas C, Robert P, Pais M, Vey A, Quiot J-M (1994) Insecticidal and cytotoxic effects of natural
and hemisynthetic destruxins. Comp Biochem Physiol Pharmacol Toxicol Endocrinol
108:195–203
Elsworth JF, Grove JF (1977) Cyclodepsipeptides from Beauveria bassiana Bals. Part
1. Beauverolides H and I. J Chem Soc Perkin Trans 1:270–273
Elsworth JF, Grove JF (1980) Cyclodepsipeptides from Beauveria bassiana. Part 2. Beauverolides
A to F and their relationship to isarolide. J Chem Soc Perkin Trans 1:1795–1799
Fan JQ, Chen XR, Hu QB (2013) Effects of destruxin A on hemocytes morphology of Bombyx
mori. J Integr Agric 12:1042–1048. doi:10.1016/s2095-3119(13)60324-x
Feudjio FT, Dornetshuber R, Lemmens M, Hoffmann O, Lemmens-Gruber R, Berger W (2010)
Beauvericin and enniatin: emerging toxins and/or remedies? World Mycotoxin J 3:415–430.
doi:10.3920/wmj2010.1245
Fiolka MJ (2008) Immunosuppressive effect of cyclosporin A on insect humoral immune
response. J Invertebr Pathol 98:287–292. doi:10.1016/j.jip.2008.03.015
Firakova S, Proksa B, Sturdikova M (2007) Biosynthesis and biological activity of enniatins.
Pharmazie 62:563–568. doi:10.1691/ph.2007.8.7600
Fornelli F, Minervini F, Logrieco A (2004) Cytotoxicity of fungal metabolites to lepidopteran
(Spodoptera frugiperda) cell line (SF-9). J Invertebr Pathol 85:74–79. doi:10.1016/j.jip.2004.
01.002
Fredenhagen A, Molleyres L-P, Bohlendorf B, Laue G (2006) Structure determination of
neoefrapeptins A to N: peptides with insecticidal activity produced by the fungus Geotrichum
candidum. J Antibiot 59:267–280. doi:10.1038/ja.2006.38
Gaumann E, Roth S, Ettlinger L, Plattner PA, Nager U (1947) Enniatin, a new antibiotic active
against Mycobacteria. Experientia 3:202–203
Grove JF (1980) Cyclodepsipeptides from Beauveria bassiana. Part 3. The isolation of
beauverolides Ba, Ca, Ja, and Ka. J Chem Soc Perkin Trans 1:2878–2880
Gupta S, Krasnoff SB, Underwood NL, Renwick JAA, Roberts DW (1991) Isolation of
beauvericin as an insect toxin from Fusarium semitectum and Fusarium moniliforme var.
subglutinans. Mycopathologia 115:185–189. doi:10.1007/bf00462223
Gupta S, Montllor C, Hwang Y-S (1995) Isolation of novel beauvericin analogs from the fungus
Beauveria bassiana. J Nat Prod 58:733–738. doi:10.1021/np50119a012
Hamill RL, Higgens CE, Boaz HE, Gorman M (1969) Structure of beauvericin, a new depsipeptide
antibiotic toxic to Artemia salina. Tetrahedron Lett 49:4255–4258
Haritakun R, Sappan M, Suvannakad R, Tasanathai K, Isaka M (2010) An antimycobacterial
cyclodepsipeptide from the entomopathogenic fungus Ophiocordyceps communis BCC 16475.
J Nat Prod 73:75–78. doi:10.1021/np900520b
Hayakawa Y, Hattori Y, Kawasaki T, Kanoh K, Adachi K, Shizuri Y, Shin-ya K (2008)
Efrapeptin J, a new down-regulator of the molecular chaperone GRP78 from a marine
Tolypocladium sp. J Antibiot 61:365–371. doi:10.1038/ja.2008.51
He H, Janso JE, Yang HY, Bernan VS, Lin SL, Yu K (2006) Culicinin D, an antitumor peptaibol
produced by the fungus Culicinomyces clavisporus, strain LL-12I252. J Nat Prod 69:736–741.
doi:10.1021/np058133r
Herrmann M, Zocher R, Haese A (1996) Enniatin production by Fusarium strains and its effect on
potato tuber tissue. Appl Environ Microbiol 62:393–398
Hiraga K, Yamamoto S, Fukuda H, Hamanaka N, Oda K (2005) Enniatin has a new function as an
inhibitor of Pdr5p, one of the ABC transporters in Saccharomyces cerevisiae. Biochem
Biophys Res Commun 328:1119–1125
Hu QB, Ren SX, Liu SY (2007a) Purification of destruxins produced by Metarhizium anisopliae
and bioassay of their insecticidal activities against grubs. Kunchong Xuebao 50:461–466
Hu QB, Ren SX, An XC, Qian MH (2007b) Insecticidal activity influence of destruxins on the
pathogenicity of Paecilomyces javanicus against Spodoptera litura. J Appl Entomol
131:262–268. doi:10.1111/j.1439-0418.2007.01159.x
Hur GH, Vickery CR, Burkart MD (2012) Explorations of catalytic domains in non-ribosomal
peptide synthetase enzymology. Nat Prod Rep 29:1074–1098. doi:10.1039/c2np20025b
Isaka M, Palasarn S, Kocharin K, Hywel-Jones NL (2007a) Comparison of the bioactive secondary
metabolites from the scale insect pathogens, anamorph Paecilomyces cinnamomeus and
teleomorph Torrubiella luteorostrata. J Antibiot 60:577–581. doi:10.1038/ja.2007.73
Isaka M, Palasarn S, Lapanun S, Sriklung K (2007b) Paecilodepsipeptide A, an antimalarial and
antitumor cyclohexadepsipeptide from the insect pathogenic fungus Paecilomyces
cinnamomeus BCC 9616. J Nat Prod 70:675–678. doi:10.1021/np060602h
Isaka M, Srisanoh U, Lartpornmatulee N, Boonruangprapa T (2007c) ES-242 derivatives and
cycloheptapeptides from Cordyceps sp. strains BCC 16173 and BCC 16176. J Nat Prod
70:1601–1604. doi:10.1021/np070357h
Isaka M, Palasarn S, Supothina S, Komwijit S, Luangsa-ard JJ (2011) Bioactive compounds from
the scale insect pathogenic fungus Conoideocrella tenuis BCC 18627. J Nat Prod 74:782–789.
doi:10.1021/np100849x
Jackson CG, Linnett PE, Beechey RB, Henderson PJF (1979) Purification and preliminary
structure analysis of the efrapeptins, a group of antibiotics that inhibit the mitochondrial
adenosine triphosphatase. Biochem Soc Trans 7:224–226
Jegorov A, Matha V, Weiser J (1990) Production of cyclosporins by entomopathogenic fungi.
Microbios Lett 45:65–69
Jegorov A, Matha V, Hradec H (1992) Detoxification of destruxins in Galleria mellonella
L. larvae. Comp Biochem Physiol C 103:227–229
Jegorov A, Sedmera P, Matha V, Simek P, Zahradnickova H, Landa Z, Eyal J (1994)
Beauverolides L and La from Beauveria tenella and Paecilomyces fumosoroseus. Phytochem-
istry 37:1301–1303
Jestoi M, Rokka M, Peltonen K (2007) An integrated sample preparation to determine
coccidiostats and emerging Fusarium-mycotoxins in various poultry tissues with LC-MS/
MS. Mol Nutr Food Res 51:625–637. doi:10.1002/mnfr.200600232
Juan C, Raiola A, Manes J, Ritieni A (2014) Presence of mycotoxin in commercial infant formulas
and baby foods from Italian market. Food Control 39:227–236. doi:10.1016/j.foodcont.2013.
10.036
Kallen J, Spitzfaden C, Zurini MGM, Wider G, Widmer H, Wuthrich K, Walkinshaw MD (1991)
Structure of human cyclophilin and its binding site for cyclosporin A determined by X-ray
crystallography and NMR spectroscopy. Nature 353:276–279
Kanaoka M, Isogai A, Murakoshi S, Ichinoe M, Suzuki A, Tamura S (1978) Bassianolide, a new
insecticidal cyclodepsipeptide from Beauveria bassiana and Verticillium lecanii. Agric Biol
Chem 42:629–635. doi:10.1271/bbb1961.42.629
Kershaw MJ, Moorhouse ER, Bateman R, Reynolds SE, Charnley AK (1999) The role of
destruxins in the pathogenicity of Metarhizium anisopliae for three species of insect. J
Invertebr Pathol 74:213–223. doi:10.1006/jipa.1999.4884
Kodaira Y (1961) Toxic substances to insects, produced by Aspergillus ochraceus and Oospora
destructor. Agric Biol Chem 25:261–262. doi:10.1271/bbb1961.25.261
Kodaira Y (1962) Studies on the new toxic substances to insects, destruxin A and B, produced by
Oospora destructor. I. Isolation and purification of destruxin A and B. Agric Biol Chem
26:36–42. doi:10.1271/bbb1961.26.36
Kovarik J (2013) From immunosuppression to immunomodulation: current principles and future
strategies. Pathobiology 80:275–281. doi:10.1159/000346960
Krasnoff SB, Gupta S (1991) Identification and directed biosynthesis of efrapeptins in the fungus
Tolypocladium geodes Gams (Deuteromycotina: Hyphomycetes). J Chem Ecol 17:1953–1962.
doi:10.1007/bf00992580
Krasnoff SB, Gupta S, St. Leger RJ, Renwick JAA, Roberts DW (1991) Antifungal and insecti-
cidal properties of the efrapeptins: metabolites of the fungus Tolypocladium niveum. J
Invertebr Pathol 58:180–188. doi:10.1016/0022-2011(91)90062-u
Krasnoff SB, Reategui RF, Wagenaar MM, Gloer JB, Gibson DM (2005) Cicadapeptins I and II:
new Aib-containing peptides from the entomopathogenic fungus Cordyceps heteropoda. J Nat
Prod 68:50–55. doi:10.1021/np0497189
Krasnoff SB, Keresztes I, Gillilan RE, Szebenyi DME, Donzelli BGG, Churchill ACL, Gibson
DM (2007) Serinocyclins A and B, cyclic heptapeptides from Metarhizium anisopliae. J Nat
Prod 70:1919–1924. doi:10.1021/np070407i
Lang G, Blunt JW, Cummings NJ, Cole ALJ, Munro MHG (2005) Hirsutide, a cyclic tetrapeptide
from a spider-derived entomopathogenic fungus, Hirsutella sp. J Nat Prod 68:1303–1305.
doi:10.1021/np0501536
Langenfeld A, Blond A, Gueye S, Herson P, Nay B, Dupont J, Prado S (2011) Insecticidal
cyclodepsipeptides from Beauveria felina. J Nat Prod 74:825–830. doi:10.1021/np100890n
Lardy H, Reed P, Lin C-HC (1975) Antibiotic inhibitors of mitochondrial ATP synthesis. Fed Proc
Fed Am Soc Exp Biol 34:1707–1710
Levy D, Bluzat A, Seigneuret M, Rigaud J-L (1995) Alkali cation transport through liposomes by
the antimicrobial fusafungine and its constitutive enniatins. Biochem Pharmacol
50:2105–2107. doi:10.1016/0006-2952(95)02045-4
Liu B-L, Tzeng Y-M (2012) Development and applications of destruxins: a review. Biotechnol
Adv 30:1242–1254. doi:10.1016/j.biotechadv.2011.10.006
Liu J, Farmer JD Jr, Lane WS, Friedman J, Weissman I, Schreiber SL (1991) Calcineurin is a
common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes. Cell 66:807–815
Luciano FB, Meca G, Manyes L, Manes J (2014) A chemical approach for the reduction of
beauvericin in a solution model and in food systems. Food Chem Toxicol 64:270–274. doi:10.
1016/j.fct.2013.11.021
Makrlik E, Toman P, Vanura P (2013a) Complexation of Pb2+ with beauvericin: an experimental
and theoretical study. Monatsh Chem 144:1461–1465. doi:10.1007/s00706-013-1054-z
Makrlik E, Toman P, Vanura P (2013b) Experimental and DFT study on the complexation of Ba2+
with beauvericin. J Radioanal Nucl Chem 295:1887–1891. doi:10.1007/s10967-012-2107-1
Makrlik E, Toman P, Vanura P (2013c) Experimental and theoretical study on the complexation of
Ca2+ with beauvericin. J Radioanal Nucl Chem 298:195–200. doi:10.1007/s10967-012-2335-
4
Makrlik E, Toman P, Vanura P (2013d) Extraction and DFT study on the complexation of Zn2+
with beauvericin. Acta Chim Slov 60:884–888
Matsuda D, Namatame I, Tomoda H, Kobayashi S, Zocher R, Kleinkauf H, Omura S (2004) New
beauveriolides produced by amino acid-supplemented fermentation of Beauveria sp. FO-6979.
J Antibiot 57:1–9. doi:10.7164/antibiotics.57.1
Mochizuki K, Ohmori K, Tamura H, Shizuri Y, Nishiyama S, Miyoshi E, Yamamura S (1993a)
The structures of bioactive cyclodepsipeptides, beauveriolides I and II, metabolites of
entomopathogenic fungi Beauveria sp. Bull Chem Soc Jpn 66:3041–3046. doi:10.1246/bcsj.
66.3041
Mochizuki K, Ohomori K, Tamura H, Shizuri Y, Nishiyama S, Miyoshi E, Yamamura S (1993b)
The structures of bioactive cyclodepsipeptides, Beauveriollides I and II, metabolites of
entomopathogenic fungi Beauveria sp. Bull Chem Soc Jpn 66:3041–3046
Molleyres L-P, Fredenhagen A, Schuez TC, Boehlendorf B, Neff S, Huang Y (2004) Production of
neoefrapeptins for use as insecticides. DE10361201A1
Momose I, Ohba S-i, Tatsuda D, Kawada M, Masuda T, Tsujiuchi G, Yamori T, Esumi H, Ikeda D
(2010) Mitochondrial inhibitors show preferential cytotoxicity to human pancreatic cancer
PANC-1 cells under glucose-deprived conditions. Biochem Biophys Res Commun
392:460–466. doi:10.1016/j.bbrc.2010.01.050
Monma S, Sunazuka T, Nagai K, Arai T, Shiomi K, Matsui R, Omura S (2006) Verticilide:

elucidation of absolute configuration and total synthesis. Org Lett 8:5601–5604. doi:10.1021/
ol0623365
Moretti A, Mule G, Ritieni A, Logrieco A (2007) Further data on the production of beauvericin,
enniatins and fusaproliferin and toxicity to Artemia salina by Fusarium species of Gibberella
fujikuroi species complex. Int J Food Microbiol 118:158–163. doi:10.1016/j.ijfoodmicro.2007.
07.004
Muellenhoff MW, Koo JY (2012) Cyclosporine and skin cancer: an international dermatologic
perspective over 25 years of experience. A comprehensive review and pursuit to define safe use
of cyclosporine in dermatology. J Dermatol Treat 23:290–304. doi:10.3109/09546634.2011.
590792
Mule G, D’Ambrosio A, Logrieco A, Bottalico A (1992) Toxicity of mycotoxins of Fusarium
sambucinum for feeding in Galleria mellonella. Entomol Exp Appl 62:17–22. doi:10.1111/j.
1570-7458.1992.tb00636.x
Nagaoka Y, Hata K, Fukata N, Uesato S, Ueda J, Higashi K, Uchida S, Fujita T, Sasaki S,
Tachikawa E (2006) Cicadapeptins from a fungus Isaria sinclairii inhibit acetylcholine-evoked
secretion of catecholamines from bovine adrenal chromaffin cells. Pept Sci 43:248
Nakajyo S, Shimizu K, Kometani A, Kato K, Kamizaki J, Isogai A, Urakawa N (1982) Inhibitory
effect of bassianolide, a cyclodepsipeptide, on drug-induced contractions of isolated smooth
muscle preparations. Jpn J Pharmacol 32:55–64. doi:10.1254/jjp.32.55
Nakajyo S, Shimizu K, Kometani A, Suzuki A, Ozaki H, Urakawa N (1983) On the inhibitory
mechanism of bassianolide, a cyclodepsipeptide, in acetylcholine-induced contraction in
guinea-pig taenia coli. Jpn J Pharmacol 33:573–582. doi:10.1254/jjp.33.573
Nakaya S, Mizuno S, Ishigami H, Yamakawa Y, Kawagishi H, Ushimaru T (2012) New rapid
screening method for anti-aging compounds using budding yeast and identification of
beauveriolide I as a potent active compound. Biosci Biotechnol Biochem 76:1226–1228.
doi:10.1271/bbb.110872
Namatame I, Tomoda H, Ishibashi S, Omura S (2004) Antiatherogenic activity of fungal
beauveriolides, inhibitors of lipid droplet accumulation in macrophages. Proc Natl Acad Sci
U S A 101:737–742. doi:10.1073/pnas.0307757100
Ohshiro T, Matsuda D, Nagai K, Doi T, Sunazuka T, Takahashi T, Rudel LL, Omura S, Tomoda H
(2009) The selectivity of beauveriolide derivatives in inhibition toward the two isozymes of
acyl-CoA: cholesterol acyltransferase. Chem Pharm Bull 57:377–381. doi:10.1248/cpb.57.377
Ohshiro T, Matsuda D, Kazuhiro T, Uchida R, Nonaka K, Masuma R, Tomoda H (2012) New
verticilides, inhibitors of acyl-CoA:cholesterol acyltransferase, produced by Verticillium
sp. FKI-2679. J Antibiot (Tokyo) 65:255–262. doi:ja201212 [pii]10.1038/ja.2012.12 [doi]
Ohyama M, Okada Y, Takahashi M, Sakanaka O, Matsumoto M, Atsumi K (2011) Structure-
activity relationship of anthelmintic cyclooctadepsipeptides. Biosci Biotechnol Biochem
75:1354–1363. doi:10.1271/bbb.110129
Omura S, Tomoda H (2002) Selection culture media for beauveriolide I or beauveriolide III
manufacture with Beauveria. WO2002077203A1
Omura S, Shiomi K, Masuma R (2004) Novel substance FKI-1033 manufacture with Verticillium
as insecticide. WO2004044214A1
Pal S, St. Leger RJ, Wu LP (2007) Fungal peptide destruxin a plays a specific role in suppressing
Papathanassiu AE, MacDonald NJ, Bencsura A, Vu HA (2006) F1F0-ATP synthase functions as a
co-chaperone of Hsp90-substrate protein complexes. Biochem Biophys Res Commun
345:419–429. doi:10.1016/j.bbrc.2006.04.104
Papathanassiu AE, MacDonald NJ, Emlet DR, Vu HA (2011) Antitumor activity of efrapeptins,
alone or in combination with 2-deoxyglucose, in breast cancer in vitro and in vivo. Cell Stress
Chaperones 16:181–193. doi:10.1007/s12192-010-0231-9
Pedras MSC, Irina Zaharia L, Ward DE (2002) The destruxins: synthesis, biosynthesis, biotrans-
formation, and biological activity. Phytochemistry 59:579–596. doi:10.1016/s0031-9422(02)
00016-x
Plattner RD, Nelson PE (1994) Production of beauvericin by a strain of Fusarium proliferatum
isolated from corn fodder for swine. Appl Environ Microbiol 60:3894–3896
Podsiadlowski L, Matha V, Vilcinskas A (1998) Detection of a P-glycoprotein related pump in
Chironomus larvae and its inhibition by verapamil and cyclosporin A. Comp Biochem Phys B
121:443–450
Prosperini A, Juan-Garcia A, Font G, Ruiz MJ (2013) Beauvericin-induced cytotoxicity via ROS
production and mitochondrial damage in Caco-2 cells. Toxicol Lett 222:204–211. doi:10.1016/
j.toxlet.2013.07.005
Putri SP, Ishido K-i, Kinoshita H, Kitani S, Ihara F, Sakihama Y, Igarashi Y, Nihira T (2013)
Production of antioomycete compounds active against the phytopathogens Phytophthora sojae
and Aphanomyces cochlioides by clavicipitoid entomopathogenic fungi. J Biosci Bioeng.
doi:10.1016/j.jbiosc.2013.10.014
Ravindra G, Ranganayaki RS, Raghothama S, Srinivasan MC, Gilardi RD, Karle IL, Balaram P
(2004) Two novel hexadepsipeptides with several modified amino acid residues isolated from
the fungus Isaria. Chem Biodivers 1:489–504. doi:10.1002/cbdv.200490043
Rivas F, Howell G, Floyd Z (2008) Orgn 441- Progress toward the synthesis of two antibacterial
heptapeptides: Cicadapeptins I and II. Abstracts of papers of the American Chemical Society,
236th National meeting of the American-Chemical-Society, Philadelphia
Rizwan-Ul-Haq M, Hu QB, Hu MY, Zhong G, Weng Q (2009) Study of destruxin B and tea
saponin, their interaction and synergism activities with Bacillus thuringiensis kurstaki against
Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae). Appl Entomol Zool 44:419–428.
doi:10.1303/aez.2009.419
Ruiz-Sanchez E, O’Donnell MJ (2012) Effects of the microbial metabolite destruxin a on ion
transport by the gut and renal epithelia of Drosophila melanogaster. Arch Insect Biochem
Physiol 80:109–122. doi:10.1002/arch.21023
Ruiz-Sanchez E, Orchard I, Lange AB (2010) Effects of the cyclopeptide mycotoxin destruxin A
on the Malpighian tubules of Rhodnius prolixus (Stal). Toxicon 55:1162–1170. doi:10.1016/j.
toxicon.2010.01.006
Rukachaisirikul V, Chantaruk S, Tansakul C, Saithong S, Chaicharernwimonkoon L,
Pakawatchai C, Isaka M, Intereya K (2006) A cyclopeptide from the insect pathogenic fungus
Cordyceps sp. BCC 1788. J Nat Prod 69:305–307. doi:10.1021/np050433l
Ryffel B, Donatsch P, Goetz U, Tschopp M (1980) Cyclosporin receptor on mouse lymphocytes.
Immunology 41:913–919
Sabareesh V, Ranganayaki RS, Raghothama S, Bopanna MP, Balaram H, Srinivasan MC, Balaram
P (2007) Identification and characterization of a library of microheterogeneous cyclohexadep-
sipeptides from the fungus Isaria. J Nat Prod 70:715–729. doi:10.1021/np060532e
Santini A, Meca G, Uhlig S, Ritieni A (2012) Fusaproliferin, beauvericin and enniatins: occur-
rence in food – a review. World Mycotoxin J 5:71–81. doi:10.3920/wmj2011.1331
Shiomi K, Matsui R, Kakei A, Yamaguchi Y, Masuma R, Hatano H, Arai N, Isozaki M, Tanaka H,
Kobayashi S, Turberg A, Omura S (2010) Verticilide, a new ryanodine-binding inhibitor,
produced by Verticillium sp. FKI-1033. J Antibiot (Tokyo) 63:77–82. doi:ja2009126 [pii]
10.1038/ja.2009.126 [doi]
tural changes in the salivary glands of Spodoptera litura (Fab.) larvae. Toxicon 51:1140–1150.
doi:10.1016/j.toxicon.2008.01.012
Steinrauf LK (1985) Beauvericin and the other enniatins. Met Ions Biol Syst 19:139–171
Strongman DB, Strunz GM, Giguere P, Yu CM, Calhoun L (1988) Enniatins from Fusarium
avenaceum isolated from balsam fir foliage and their toxicity to spruce budworm larvae,
Choristoneura fumiferana (Clem.) (Lepidoptera: tortricidae). J Chem Ecol 14:753–764.

doi:10.1007/bf01018770
Supothina S, Isaka M, Kirtikara K, Tanticharoen M, Thebtaranonth Y (2004) Enniatin production
by the entomopathogenic fungus Verticillium hemipterigenum BCC 1449. J Antibiot
57:732–738
Suzuki A, Kanaoka M, Isogai A, Murakoshi S, Ichinoe M, Tamura S (1977) Bassianolide, a new
Lett 18:2167–2170. doi:10.1016/s0040-4039(01)83709-6
Sy-Cordero AA, Pearce CJ, Oberlies NH (2012) Revisiting the enniatins: a review of their
isolation, biosynthesis, structure determination and biological activities. J Antibiot
65:541–549. doi:10.1038/ja.2012.71
Tomoda H, Omura S (2007) Potential therapeutics for obesity and atherosclerosis: inhibitors of
neutral lipid metabolism from microorganisms. Pharmacol Ther 115:375–389. doi:10.1016/j.
pharmthera.2007.05.008
Tomoda H, Huang XH, Cao J, Nishida H, Nagao R, Okuda S, Tanaka H, Omura S, Arai H, Inoue K
(1992) Inhibition of acyl-CoA: cholesterol acyltransferase activity by cyclodepsipeptide anti-
biotics. J Antibiot 45:1626–1632. doi:10.7164/antibiotics.45.1626
Tomoda H, Omura S, Takahashi T, Doi T, Hijikuro I, Imuta S, Matsuda D, Oki T (2010)
Beauveriolide having ACAT-2 inhibitory activity. JP2010126454A
Vaclavik L, Vaclavikova M, Begley TH, Krynitsky AJ, Rader JI (2013) Determination of multiple
mycotoxins in dietary supplements containing green coffee bean extracts using ultrahigh-
performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). J Agric
Food Chem 61:4822–4830. doi:10.1021/jf401139u
Vazquez MJ, Albarran MI, Espada A, Rivera-Sagredo A, Diez E, Hueso-Rodriguez JA (2005) A
new destruxin as inhibitor of vacuolar-type H+-ATPase of Saccharomyces cerevisiae. Chem
Biodivers 2:123–130
Vey A, Matha V, Dumas C (2002) Effects of the peptide mycotoxin destruxin E on insect
hemocytes and on dynamics and efficiency of the multicellular immune reaction. J Invertebr
Pathol 80:177–187. doi:10.1016/s0022-2011(02)00104-0
Vilcinskas A, Matha V, Gotz P (1997a) Effects of the entomopathogenic fungus Metarhizium
anisopliae and its secondary metabolites on morphology and cytoskeleton of plasmatocytes
isolated from the greater wax moth, Galleria mellonella. J Insect Physiol 43:1149–1159.
doi:10.1016/s0022-1910(97)00066-8
Vilcinskas A, Matha V, Gotz P (1997b) Inhibition of phagocytic activity of plasmatocytes isolated
from Galleria mellonella by entomogenous fungi and their secondary metabolites. J Insect
Physiol 43:475–483. doi:10.1016/s0022-1910(96)00120-5
Vilcinskas A, Jegorov A, Landa Z, Goatz P, Matha V (1999) Effects of beauverolide L and
cyclosporin A on humoral and cellular immune response of the greater wax moth, Galleria
mellonella. Comp Biochem Physiol C 122:83–92
Vining LC, Taber WA (1962) Isariin, a new depsipeptide from Isaria cretacea. Can J Chem
40:1579–1584. doi:10.1139/v62-239
Vongvanich N, Kittakoop P, Isaka M, Trakulnaleamsai S, Vimuttipong S, Tanticharoen M,
Thebtaranonth Y (2002) Hirsutellide A, a new antimycobacterial cyclohexadepsipeptide
from the entomopathogenic fungus Hirsutella kobayasii. J Nat Prod 65:1346–1348. doi:10.
1021/np020055+
Molecules 17:2367–2377. doi:10.3390/molecules17032367
Weigelt S, Huber T, Hofmann F, Jost M, Ritzefeld M, Luy B, Freudenberger C, Majer Z, Vass E,
Greie J-C, Panella L, Kaptein B, Broxterman QB, Kessler H, Altendorf K, Hollosi M, Sewald
N (2012) Synthesis and conformational analysis of efrapeptins. Chemistry 18:478–487
Weiser J, Matha V (1988) The insecticidal activity of cyclosporines on mosquito larvae. J
Invertebr Pathol 51:92–93. doi:10.1016/0022-2011(88)90092-4
Wiesinger D, Borel JF (1980) Studies on the mechanism of action of cyclosporin

A. Immunobiology (Stuttgart) 156:454–463. doi:10.1016/s0171-2985(80)80078-7
Witter DP, Chen Y, Rogel JK, Boldt GE, Wentworth P Jr (2009) The natural products
Beauveriolide I and III: a new class of β-amyloid-lowering compounds. Chembiochem
10:1344–1347. doi:10.1002/cbic.200900139
Xu Y, Zhan J, Wijeratne EMK, Burns AM, Gunatilaka AAL, Molnar I (2007) Cytotoxic and
antihaptotactic beauvericin analogues from precursor-directed biosynthesis with the insect
pathogen Beauveria bassiana ATCC 7159. J Nat Prod 70:1467–1471. doi:10.1021/np070262f
Xu Y, Rozco R, Wijeratne EMK, Espinosa-Artiles P, Gunatilaka AAL, Stock SP, Molnar I (2009)
Biosynthesis of the cyclooligomer depsipeptide bassianolide, an insecticidal virulence factor of
Beauveria bassiana. Fungal Genet Biol 46:353–364. doi:10.1016/j.fgb.2009.03.001
Yang M, Wang Y, Liu X, Wu J (2013) Synthesis and anti-tumor activity of cyclodepsipeptides
paecilodepsipeptide A. Adv Mater Res 643:92–95. doi:10.4028/www.scientific.net/AMR.643.
92
Yi F, Zou C, Hu Q, Hu M (2012) The joint action of destruxins and botanical insecticides
(rotenone, azadirachtin and paeonolum) against the cotton aphid, Aphis gossypii Glover.
Molecules 17:7533–7542. doi:10.3390/molecules17067533
Yu D, Xu F, Gage D, Zhan J (2013a) Functional dissection and module swapping of fungal
cyclooligomer depsipeptide synthetases. Chem Commun 49:6176–6178. doi:10.1039/
c3cc42425a
Yu D, Xu F, Zi J, Wang S, Gage D, Zeng J, Zhan J (2013b) Engineered production of fungal
anticancer cyclooligomer depsipeptides in Saccharomyces cerevisiae. Metab Eng 18:60–68.
doi:10.1016/j.ymben.2013.04.001
Zhang L, Yan K, Zhang Y, Huang R, Bian J, Zheng C, Sun H, Chen Z, Sun N, An R, Min F,
Zhao W, Zhuo Y, You J, Song Y, Yu Z, Liu Z, Yang K, Gao H, Dai H, Zhang X, Wang J, Fu C,
Pei G, Liu J, Si Z, Goodfellow M, Jiang Y, Kuai J, Zhou G, Chen X (2007) High-throughput
synergy screening identifies microbial metabolites as combination agents for the treatment of
fungal infections. Proc Natl Acad Sci U S A 104:4606–4611. doi:10.1073/pnas.0609370104
Zhang W, Sun TT, Mei D, Wang JF, Li YX (2008) Synthesis of protected (2S,4R)-2-amino-4-
methyldecanoic acid, a proposed component of culicinins. Chin Chem Lett 19:1068–1070.
doi:10.1016/j.cclet.2008.06.027
Zhang W, Sun T-T, Li Y-X (2009) Synthesis of the C-terminal pentapeptide of the peptaibol
culicinins. J Pept Sci 15:366–368. doi:10.1002/psc.1124
Zhang W, Ding N, Li Y (2011) An improved synthesis of (2S,4S)- and (2S,4R)-2-amino-4-
methyldecanoic acids: assignment of the stereochemistry of culicinins. J Pept Sci
17:576–580. doi:10.1002/psc.1376
Zhang D, Li S, Lu R, Li K, Luo F, Peng F, Hu F (2012) Influence of incubation time on metabolites
in mycelia of Paecilomyces militaris. Weishengwu Xuebao 52:1477–1488
Chapter 9
Optimization of the Cyclodepsipeptidic
Destruxin Recovery from Broth Culture
of Metarhizium anisopliae
and Its Augmentation by Precursor
Supplementation
K. Sowjanya Sree and V. Padmaja
9.1 Introduction
Cyclic hexadepsipeptidic secondary metabolite, destruxin (dtx), is a major class of

mycotoxins produced by the entomopathogenic fungus Metarhizium anisopliae
(Metch.). This cyclic metabolite comprises of five amino acids, viz., proline
(Pro), isoleucine (Ile), methyl valine (MeVal), methyl alanine (MeAla), and β
alanine (βAla), and a hydroxyl acid, viz., sodium acetate or pipecolic acid (Pedras
et al. 2002). Individual dtxs differ in the hydroxyl acid, N-methylation, and R group
of the amino acid residues (Pedras et al. 2002). The genes responsible for the
biosynthesis of destruxin from Metarhizium species were revealed in a recent
study by Wang et al. (2012). Studies on the isotopically labeled precursors (13C)
administered to M. anisopliae cultures showed that L-methionine is the methyl
group donor in the process of N-methylation of MeAla and MeVal residues of dtx
(Jegorov et al. 1993).
Dtx A and E are the most predominant forms in the cultures of M. anisopliae and
possess insecticidal properties against lepidopterans (Sree et al. 2008) and also
against a large variety of insects as reviewed by Pedras et al. (2002). Dtxs cause an
initial tetanic paralysis in the treated insect larvae, which is attributed to muscle
depolarization by direct opening of the Ca2+ channels in the membrane (Samuels
et al. 1988). It is reported that dtxs mediate specific downregulation of anti-
microbial peptides (AMPs) by means of targeting the insect’s innate immune signal-
ing pathway (Pal et al. 2007). Impressive changes in the status of antioxidants, viz.,
catalase, total peroxidase, ascorbate peroxidase, superoxide dismutase, glutathione,
K.S. Sree (*)

V. Padmaja
Department of Botany, Andhra University, Visakhapatnam 530003, India

DOI 10.1007/978-3-319-14499-3_9
208 K.S. Sree and V. Padmaja
ascorbate, levels of lipid peroxidation, and lipoxygenase enzyme, and the ultra-
structural changes in the salivary gland membrane have been shown in the 9-day-old
larvae of Spodoptera litura upon crude dtx treatment (Sree and Padmaja 2008a, b).
It is well documented that the recovery of dtxs is influenced by the component
type and ratio, usually carbon and nitrogen, in the culture medium (Liu et al. 2000;
Wang et al. 2004) and also by other factors like pH and temperature
(Hu et al. 2006). Optimization of media components and culture conditions through
factorial (central composite design, CCD) and response surface method (RSM)
is a common practice in biotechnology (Chen 1996; Prakash et al. 2008; Rao
et al. 1993). In order to obtain optimum yield of a secondary metabolite, destruxin
as in the present study, development of a suitable medium and culture conditions is
obligatory. Statistical optimization not only allows quick screening of a large
experimental domain but also reflects the role of each of the components. Basically,
this optimization process involves three major steps: performing the statistically
designed experiments, estimating the coefficients in a mathematical model, and
predicting the response and adequacy of the model. Using the mathematical model,
the levels of variables giving maximum response can be determined.
The aim of the present study was to optimize the conditions involved in the
recovery of dtx A and E in Czapek–Dox (CZ) liquid medium by using CCD and
RSM and also to evaluate the influence of nutrient supplements involved in dtx
biosynthesis like amino acid and hydroxyl acid precursors on dtx A and E recovery.
The biochemical profile of M. anisopliae grown on supplemented nutrient medium
in terms of acid phosphatase, alkaline phosphatase, and esterase was understood in
relation to the recovery of the secondary metabolite.
9.2 Materials and Methods
9.2.1 Chemicals
All the solvents used for secondary metabolite extraction, detection, and quantifi-
cation were of high performance liquid chromatography (HPLC) grade from
Qualigens Fine Chemicals, India. The components of fungal culture medium
were from HiMedia (India) Ltd. The chemicals used for enzyme analysis were
from Merck (India) Ltd. and Sigma-Aldrich Corporation (India).
9.2.2 Fungal Strains
The fungal strain M-19 of M. anisopliae was an ARSEF, Ithaca, collection

(ARSEF-1080) isolated from the cadavers of Heliothis spp. Sree et al. (2008)
have shown that the recovery and insecticidal activity of destruxin from this strain
9 Optimization of the Cyclodepsipeptidic Destruxin Recovery from Broth. . . 209
is very high. The propagules of these fungal strains were preserved in 30 % glycerol
under low temperature for long-term storage. The cultures were grown on SDAY at
25 C in the dark. Slants fully covered with spores, usually after 9 days of
inoculation, were used for experimentation. Germination of spores on SDAY tested
prior to experimentation revealed more than 95 % value.
9.2.3 Optimization of Variables Using RSM
The RSM was used to optimize the medium components and culture conditions for
the growth of M-19 strain of M. anisopliae. Based on the earlier reports (Liu
et al. 2000; Wang et al. 2004; Hu et al. 2006) and our observations, carbon and
nitrogen ratio in the medium, initial pH, and temperature were selected as indepen-
dent variables in the CCD experiment. Each factor had five coded levels (α, 1,
0, +1, +) (Table 9.1). The response was the amount of dtx A and E recovered from
the culture filtrate after 7 days of incubation. The CCD contained 18 runs as shown
in Table 9.2.
9.2.4 Statistical Analysis and Modeling
The results of RSM were used to fit a second-order polynomial equation.
Y ¼ β 0 þ β1 X1 þ β2 X2 þ β 3 X 3 þ β 1 β 1 X 1 2 þ β 2 β 2 X 2 2 þ β 3 β 3 X 3 2 þ β 1 β 2 X 1 X 2
þ β1 β3 X1 X3 þ β2 β3 X 2 X3
where Y is the predicted response, β0 is the intercept, β1, β2, β3 are the linear
coefficients, β1β1, β2β2, β3β3 are the squared coefficients, and β1β2, β1β3, β2β3 are
the interaction coefficients. X1, X2, and X3 are the independent variables as given in
Table 9.1.
The statistical significance of the model equation was determined by analysis of
variance (ANOVA) (α ¼ 0.05), and the proportion of variance explained by the
model was given by the multiple coefficient of determination, R squared (R2) value.
STATISTICA version 6.0 was used for the design and analysis of this experiment.
9.2.5 In Vitro Production and Recovery of dtx A and E
The crude dtx produced in vitro was extracted according to Wang et al. (2003).
Briefly, 1.5 ml of spore suspension (2 107 conidia/ml) of M. anisopliae was
introduced into 150 ml conical flasks charged with 50 ml of CZ liquid medium
Table 9.1 Range of values of the independent variables for response surface methodology
Levels
Independent variable α 1 0 +1 +α
X1—CN ratio 0.636 2.0 4.0 6.0 7.364
X2—pH 5.318 6.0 7.0 8.0 8.682
X3—temperature 16.591 20.0 25.0 30.0 33.409
Table 9.2 Experimental design and results of CCD of response surface method
Expt. CN Temperature Dtx A (mg/l) Dtx E (mg/l)

runs ratio pH ( C) Observed Predicted Observed Predicted
1 2.0 6.0 20.0 5.99 5.47 5.13 4.65
2 2.0 6.0 30.0 5.97 5.57 5.06 4.71
3 2.0 8.0 20.0 6.16 5.53 5.24 4.70
4 2.0 8.0 30.0 6.19 5.66 5.09 4.63
5 6.0 6.0 20.0 2.10 3.34 1.15 2.40
6 6.0 6.0 30.0 2.01 3.34 1.26 2.60
7 6.0 8.0 20.0 2.15 3.26 1.36 2.50
8 6.0 8.0 30.0 2.05 3.28 1.28 2.55
9 0.6 7.0 25.0 1.8 3.38 1.01 2.50
10 7.3 7.0 25.0 2.16 0.41 1.46 1.13
11 4.0 5.3 25.0 6.75 6.11 5.96 5.29
12 4.0 8.6 25.0 6.48 6.12 5.75 5.30
13 4.0 7.0 16.5 6.57 6.20 5.67 5.24
14 4.0 7.0 33.4 6.93 6.30 6.03 5.34
15 4.0 7.0 25.0 7.55 7.71 6.26 6.38
16 4.0 7.0 25.0 7.67 7.71 6.37 6.38
17 4.0 7.0 25.0 7.79 7.71 6.41 6.38
18 4.0 7.0 25.0 7.66 7.71 6.3 6.38
along with the differing amounts of glucose and peptone in order to make up to the
required CN ratio (Wang et al. 2004). After incubation at varying combinations of
pH and temperature (Table 9.2) for 7 days at 200 rpm in a rotary shaker, the cultures
were filtered through three layers of filter papers and then through Whatman
no. 1 filter paper. The culture filtrate was extracted two times for 12 h with
dichloromethane–ethyl acetate (1:1, v/v). The solvent was evaporated in vacuo,
and the crude extract was dissolved in 1 ml absolute methanol for HPLC analysis.
Each experiment was repeated three times with three replicates each.
The reverse phase HPLC (RP-HPLC) was carried out according to Kershaw
et al. (1999). A Shimadzu HPLC apparatus equipped with a Spherisorb C18 column
was used. The mobile phase, i.e., 50 % acetonitrile, was run isocratically at a flow rate
of 1 ml/min. Absorbance peaks obtained in the UV range at 210 nm were identified
by comparison with the chromatogram published by Kershaw et al. (1999), and
dtx A and E were quantified by peak area measurement with the help of the inbuilt
software in HPLC system.
9.2.6 Destruxin Biosynthesis Precursors as Supplements

to the Optimized Medium
The influence of different dtx biosynthesis precursors on dtx recovery from

M. anisopliae was investigated by the addition of 0.02 % of amino acid (β-alanine,
isoleucine, proline, valine, methionine) and hydroxyl acid (sodium acetate) supple-
ments to the optimized medium. The dtx A and E were recovered and quantified as
mentioned above. Each experiment was repeated three times with three replicates
each.
9.2.7 Biochemical Profile of M. anisopliae Grown

on Supplemented Nutrient Medium
The activity of acid phosphatase, alkaline phosphatase, and esterase were studied in
M. anisopliae grown on optimized medium supplemented with various dtx bio-
synthesis precursors along with the control which was grown on optimized medium
without any supplements. Each experiment was repeated three times with three
replicates each.
9.2.7.1 Acid Phosphatase
The acid phosphatase enzyme was extracted by homogenizing 1 g of the fresh

fungal pellets in 10 ml of ice-cold 50 mM citrate buffer (pH 5.3) in a prechilled
mortar and pestle. The homogenate was centrifuged at 10,000g for 10 min, and
the supernatant thus obtained was used as the enzyme source.
Activity of the enzyme was assayed spectrophotometrically by adding 0.5 ml of
the enzyme extract to 3 ml of the substrate solution (1.49 mM EDTA, 0.84 mM
citric acid, and 0.03 mM p-nitrophenylphosphate in distilled water, pH 5.3). This
was incubated at 37 C for 15 min, and then 0.5 ml of this mixture was added to
9.5 ml of 0.085 N sodium hydroxide solution. The absorbance of the resulting
solution was read at 405 nm. The activity was measured using a standard curve of
p-nitrophenol (Lowry et al. 1954).
9.2.7.2 Alkaline Phosphatase
The alkaline phosphatase enzyme was extracted by homogenizing 1 g of the fresh

fungal pellets in 10 ml of ice-cold 50 mM glycine NaOH buffer (pH 10.4) in a
prechilled mortar and pestle. The homogenate was centrifuged at 10,000g for
10 min, and the supernatant thus obtained was used as the enzyme source.
Activity of the enzyme was assayed spectrophotometrically by adding 0.5 ml of
the enzyme extract to 3 ml of the substrate solution (0.15 mM glycine, 0.004 mM
magnesium chloride, and 0.069 mM p-nitrophenylphosphate in 0.1 N sodium
hydroxide, pH 10.4). This mixture was incubated at 37 C for 15 min, and then
0.5 ml of this mixture was added to 9.5 ml of 0.085 N sodium hydroxide solution.
The absorbance of the resulting solution was read at 405 nm. The activity was
measured using a standard curve of p-nitrophenol (Lowry et al. 1954).
9.2.7.3 Esterase
The enzyme extract was prepared by homogenizing 1 g of the fresh pellets in 5 ml

of the solution containing 10 mM sodium phosphate buffer (pH 9.5), 1 mM EDTA,
and 1 mM β-mercaptoethanol. The homogenate was centrifuged at 10,000g for
10 min, and the supernatant was further used as the enzyme source.
Spectrophotometric assay of esterase was carried out according to Sparks
et al. (1979) with slight modifications. Initially, 0.5 ml of the enzyme extract was
allowed to hydrolyze 1 ml of 0.001 M α-naphthyl acetate in 10 mM sodium
phosphate buffer (pH 9.5). The mixture was incubated at 37 C for 15 min, and
then the reaction was stopped by the addition of 0.25 ml of stop solution (3.4 % SDS
along with 0.8 % Fast Blue B salt solution). As a result, a Fast blue B dye complex
was formed with α-naphthol. The absorbance of the resultant was read at 620 nm.
The activity was calculated using a standard graph of α-naphthol prepared in the
same way.
9.3 Results
9.3.1 Optimal Conditions for dtx A and E Recovery
The results of CCD experiments for studying the effect of three independent vari-
ables are presented along with the observed and predicted responses in Table 9.2.
The 3D response surface plots (Figs. 9.1 and 9.2) depict the interaction between the
independent variables and their effect on the amount of dtx recovery. In these
figures, the relative effect of any of the two variables is shown keeping the third one
constant. The mid value of each of the variable was taken as a constant.
Fig. 9.1 Response surface plots indicating the effect of (a) CN ratio and pH, (b) temperature and
CN ratio, and (c) pH and temperature on the recovery of dtx A from the culture filtrates of
Metarhizium anisopliae
The results of ANOVA are given in Table 9.3. The regression equations obtained
after the ANOVA gave the level of dtx A and E recovery as a function of the initial
values of CN ratio, pH, and temperature.
The critical optimized conditions for dtx A recovery were CN ratio, 3.48; pH,
0.01; and temperature, 25.18 C and for dtx E recovery were CN ratio, 3.46; pH,
6.99; and temperature, 25.14 C. In both Figs. 9.1 and 9.2, the highest contour
corresponded to the dtx A and E recovered under the optimum values of CN ratio,
pH, and temperature. At the optimum values for dtx A, 8.91 mg/l of dtx A was
recovered and at that of dtx E, 8.1 mg/l of dtx E was recovered. These values are
much higher than the different combinations designed (Table 9.2). Dtx A being the
most predominant form of dtx in the culture filtrate, the medium, and culture
conditions optimized for dtx A recovery was used for further experimentation.
a b
8 8
6 6
4
4
2
2
Dtx E
0
0
Dtx E
–2
–4 –2
–6 –4
–8 –6
–8
0
9.
8. 5
8
8.
7 8
7. 0
6 7
5
5 6
36
0
4
7.
34
5
3 5
32
6.
o
pH
ati 4
0
30
2 r
6.
28
1 CN CN 3
5. 5
26
e
5.
0
ur
0
24
ra 2
rat
22
tio e
20
1
mp
18
0
16
Te
14
c
7
6
4
Dtx E
1
36
34
32
9.
0
30
8.
28
5
8.
Te
0
26
7.
m
24
5
7.
pe
22
0
6.
ra pH
20
tu
6.
18
re
0
5.
16
5
14
5.
0
Fig. 9.2 Response surface plots indicating the effect of (a) CN ratio and pH, (b) temperature and
CN ratio, and (c) pH and temperature on the recovery of dtx E from the culture filtrates of
Metarhizium anisopliae
Table 9.3 ANOVA for the CCD of dtx A and E recovery

Dtx Source Sum of square Degrees of freedom Mean square F-value P-value
Dtx A Model 86.10 9 86.10 39.69 0.05
Error 1.73 8 0.22
Total 96.24 17
Dtx E Model 71.14 9 71.14 32.7 0.05
Error 1.74 8 0.21
Total 84.82 17
Dtx A: R2 ¼ 0.819; R2 Adj ¼ 0.616
Dtx E: R2 ¼ 0.794; R2 Adj ¼ 0.564
25
Dtx A
Dtx E
20
Dtx recovered (mg L-1)
15
10
0
Control Val Na acetate Ile Pro b-Ala Met
Supplements to CZ CN 3 medium
Fig. 9.3 Influence of amino acid and hydroxyl acid supplements on recovery of dtx A and E from
M. anisopliae grown in optimized CZ culture medium and conditions (CN ratio 3.48, pH 7.01,
temperature 25.18 C). The error bar corresponds to the standard error
9.3.2 Destruxin Biosynthesis Precursors as Supplements

to the Optimized Medium
The dtx biosynthesis precursors used in the present study significantly enhanced the
production and recovery of both dtx A and E. The maximum recovery of dtx A and
E were from the optimized medium supplemented with β-alanine. These values
(dtx A, 18.9 mg/l, and dtx E, 15.3 mg/l) were twofold higher than the controls which
were grown on unsupplemented CZ (CN ratio 3.48) medium. The addition of
isoleucine or proline to the optimized medium also showed better values compared
to controls. On the other hand, methionine supplementation recorded least influence
on dtx A and E recovery (Fig. 9.3).
9.3.3 Biochemical Profile of M-19 Strain Grown

on Supplemented Medium
The activity of acid phosphatase, alkaline phosphatase, and esterase in the fungal
pellets derived from the media supplemented with different amino acids showed
significant variation. Activity of these enzymes in the fungal pellets was directly
proportional to the dtx recovery from the respective culture filtrates. The activity of
Acid phosphatase activity

5 Alkaline phosphatase activity
Esterase activity
-1
mmoles min mg protein
3
-1
0
Control Val Na acetate Ile Pro b-Ala Met
Supplements to CZ CN 3 medium
Fig. 9.4 Activity of acid phosphatase, alkaline phosphatase, and esterase enzymes in the pellets of
M. anisopliae grown on amino acid and hydroxyl acid supplemented optimized CZ culture
medium and conditions (CN ratio 3.48, pH 7.01, temperature 25.18 C). The error bar corresponds
to the standard error
all the three enzymes was maximum in the fungal pellets grown in optimized
medium supplemented with β-alanine (Fig. 9.4).
9.4 Discussion
The RSM used in the current investigation revealed the optimized levels of the three
independent variables—CN ratio, pH, and temperature—at which the recovery of
dtx A and E was appreciably high. 81.9 % similarity in case of dtx A and 79.4 %
similarity in case of dtx E were observed between the predicted and the observed
experimental results from the R2 values which reflected the accuracy and appli-
cability of RSM to optimize the process of dtx A and E recovery.
A significant positive correlation between the recovery of dtx A and E and the
culture conditions has been shown in the present study. This is a useful parameter as
detection of one dtx is enough to screen for the dtx productive strains. This result is
in agreement with that of Hu et al. (2006) with respect to recovery of dtx A and
B. This suggests that the encoded genes for dtx biosynthesis are clustered in the
genome of M. anisopliae (Wang et al. 2012) and are upregulated at the same level
(Wang et al. 2004). This concept of toxin gene clustering has also been explained
with respect to other plant toxins (Seo et al. 2001).
The addition of the five constituent amino acids and the hydroxyl acid, sodium
acetate, which are the building blocks of this cyclic depsipeptide increased dtx
production in the optimized culture medium and conditions. A maximum of
twofold increase over the control upon supplementation with β-alanine is in accor-
dance with its reported major role in the ring formation step of the dtx biosynthesis
process (Pedras et al. 2002). Apart from this, Pro-Ile amide bond formation in the
requisite linear hexadepsipeptide precursor for cyclization is also an important step
in dtx biosynthesis (Pedras et al. 2002). As reported by Jegorov et al. (1993), the
cyclic structure of dtx is responsible for its biological activity, and this depsipeptide
is inactivated upon ring opening. Hence, the addition of β-alanine, proline, and
isoleucine showed a significant positive influence on recovery of the cyclodepsi-
peptides, dtx A and E.
As reported in our earlier studies, increase in biomass of the M-19 strain of
M. anisopliae showed an increment in dtx A and E production (Sree et al. 2008),
suggesting positive relationship between the overall metabolism of the fungus and
dtx recovery. The enzymes, viz., acid phosphatase, alkaline phosphatase, and
esterase, are involved actively in cell cycle and cell differentiation which are the
major processes involved in the increase in biomass of an organism (Wyckoff 1987;
Vincent et al. 1992). As these enzymes play a key role in the metabolism of a cell,
the increase in the activity of these enzymes suggested a corresponding increment
in metabolism of the fungus which in turn augmented the production of dtx A and
E. This hike was maximum with respect to β-alanine supplementation, which is
involved in the key cyclization step of dtx biosynthesis.
Findings of the present study on the dtx A and E recovery from the cultures of
M. anisopliae by manipulating substrate profile and production strategies would be
of use in mycopesticide development for effective pest management.
Acknowledgments The authors acknowledge Dr. Richard A. Humber, USDA-ARS, ARSEF,

Ithaca, for providing the isolate of M. anisopliae (Metch.) and Prof. YLN Murthy, School of
Chemistry, Andhra University, for his help in the HPLC work.
References
Chen HC (1996) Optimizing the concentrations of carbon, nitrogen and phosphorus in a citric acid
fermentation with response surface method. Food Biotechnol 10:13–27
Hu QB, Ren SX, Wu JH, Chang JM, Musa PD (2006) Investigation of destruxin A and B from
80 Metarhizium strains in China and the optimization of culture conditions for the strain
MaQ10. Toxicon 48:491–498
Jegorov A, Sedmera P, Matha V (1993) Biosynthesis of destruxins. Phytochemistry 33:1403–1405
Kershaw MJ, Moorhouse ER, Bateman RP, Reynolds SE, Charnley AK (1999) The role of
destruxins in the pathogenicity of Metarhizium anisopliae for three species of insect.
Liu BL, Chen JW, Tzeng YM (2000) Production of cyclodepsipeptides destruxin A and B from
Metarhizium anisopliae. Biotechnol Prog 16:993–999
Lowry OH, Roberts NR, Wu MR, Hixon WS, Crawford EJ (1954) The quantitative histochemistry
of brain. II. Enzyme measurements. J Biol Chem 207:19–37
Pal S, St. Leger RJ, Wu LP (2007) Fungal peptide destruxin A plays a specific role in suppressing
Prakash GVSB, Padmaja V, Kiran RRS (2008) Statistical optimization of process variables for the
large-scale production of Metarhizium anisopliae conidiospores in solid-state fermentation.
Bioresour Technol 99:1530–1537
Rao PV, Jayaraman K, Lakshmanan CM (1993) Production of lipase by Candida rugosa in
solid state fermentation. 2: Medium optimization and effect of aeration. Process Biochem
28:391–395
Samuels RI, Reynolds SE, Charnley AK (1988) Calcium channel activation of insect muscle by
destruxins, insecticidal compounds produced by the entomopathogenic fungus Metarhizium
anisopliae. Comp Biochem Physiol 90:403–412
Seo JA, Proctor RH, Plattner RD (2001) Characterization of four clustered and coregulated genes
associated with fumonisin biosynthesis in Fusarium verticillioides. Fungal Genet Biol 34:
155–165
Sparks TC, Willis WS, Shorey HH, Hammock BD (1979) Hemolymph juvenile hormone esterase
activity in synchronous last instar larvae of the cabbage looper, Trichoplusia ni. J Insect
Physiol 25:125–132
Sree KS, Padmaja V (2008a) Destruxin from Metarhizium anisopliae induces oxidative stress
effecting larval mortality of the polyphagous pest Spodoptera litura. J Appl Entomol 132:
68–78
Sree KS, Padmaja V (2008b) Oxidative stress induced by destruxin from Metarhizium anisopliae
Sree KS, Padmaja V, Murthy YLN (2008) Insecticidal activity of destruxin, a mycotoxin from
Metarhizium anisopliae (Hypocreales) strains against Spodoptera litura (Lepidoptera:
Noctuidae) larval stages. Pest Manag Sci 64:119–125
Vincent JB, Crowder MW, Averill BA (1992) Hydrolysis of phosphate monoesters: a biological
problem with multiple chemical solutions. Trends Biochem Sci 17:105–110
Wang CS, Skrobek A, Butt TM (2003) Concurrence of losing a chromosome and the ability to
produce destruxins in a mutant of Metarhizium anisopliae. FEMS Microbiol Lett 226:373–378
Wang CS, Skrobek A, Butt TM (2004) Investigations on the destruxin production of the
entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 85:168–174
Wang B, Kang Q, Lu Y, Bai L, Wang C (2012) Unveiling the biosynthetic puzzle of destruxins in
Metarhizium species. Proc Natl Acad Sci USA 109:1287–1292
Wyckoff HW (1987) Structure of Escherichia coli alkaline phosphatase determined by X-ray
diffraction. In: Torriani-Gorini A, Rothman FG, Silver S, Wright A, Yagil E (eds) Phosphatase
metabolism and cellular regulation in microorganisms. America Society for Microbiology,
Washington, DC, pp 118–126
Chapter 10
Beauveria bassiana: Biocontrol Beyond
Lepidopteran Pests
H.B. Singh, Chetan Keswani, Shatrupa Ray, S.K. Yadav, S.P. Singh,
S. Singh, and B.K. Sarma
10.1 Introduction
Beauveria bassiana, the anamorph stage of Cordyceps bassiana, is a facultative

cosmopolitan entomopathogen with an extremely broad host range. First discov-
ered by Agostino Bassi de Lodi (Keswani et al. 2013) in larval silkworms, the
fungus grows as a white (hyaline) mold producing single-celled, haploid, and
hydrophobic conidia. RNA-sequence transcriptomic studies have revealed the
startling ability of this fungus to adapt to varied environmental niches including
survival and interactions outside the insect host (Xiao et al. 2012). Thus, the
ecological habitat of this entomopathogen extends from the simple insect–host
interaction to a broader perspective including plant rhizosphere with a well-
equipped growth-promotion attribute (Bruck 2010). A diverse array of plant
groups, including the agronomic, medicinal, and cash crops, serve as a host for
the endophytic form of this fungus (Ownley et al. 2008; Gurulingappa
et al. 2010).
Microbial control of plant pathogens and insect pests not only reduces the
dependence on chemical pesticides but also increases sustainability of agriculture.
Although the number of registered microbial products has increased in recent
years, many potential biopesticides either have not been developed for
H.B. Singh (*) • B.K. Sarma

Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras
Hindu University, Varanasi 221005, India
C. Keswani • S.P. Singh
Faculty of Science, Department of Biochemistry, Banaras Hindu University, Varanasi 221005,
India
S. Ray • S.K. Yadav • S. Singh
Faculty of Science, Department of Botany, Banaras Hindu University, Varanasi 221005, India

DOI 10.1007/978-3-319-14499-3_10
220 H.B. Singh et al.
Table 10.1 Various host ranges of B. bassiana

Fungi Bacteria Pests
Rhizoctonia solani Xanthomonas (Coleoptera order)
axonopodis Lathrobium brunnipes, Calvia
pv. malvacearum quattuordecimguttata, Phytodecta
olivacea, Otiorhynchus sulcatus, Sitona
lineatus, S. sulcifrons, S. macularius,
S. hispidulus, Anthonomus pomorum,
Hylastes ater
Pythium ultimum, Clostridium (Hymenoptera order)
P. debaryanum, P. myriotylum perfringens Ichneumonidae, Lasius fuliginosus,
Vespula spp., Bombus pratorum
Sclerotinia sclerotiorum Listeria (Heteroptera order)
monocytogenes Picromerus bidens, Anthocoris
nemorum
Alternaria solani, A. tenuis Yersinia (Homoptera order)
enterocolitica Eulecanium spp.
Colletotrichum Salmonella (Diptera order)
gloeosporioides enterica Leria serrata
Fusarium oxysporum, Shigella (Lepidoptera order)
F. moniliforme, dysenteriae Hepialus spp., Hypocrita jacobaea,
F. graminearum, F. avenaceum Cydia nigricana
Aspergillus niger, Staphylococcus
A. parasiticus aureus
Septoria nodorum Enterococcus
faecium
commercial use or have had limited success due to their pathogen or pest
specificity. Moreover, its inconsistent performance across environments, or a
lack of understanding of the mechanism(s) of biocontrol, results into ineffective
use. Hypocrean fungi, such as B. bassiana, offer a quick respite to this problem as
they become established as epiphytes and endophytes, thereby enhancing the
induced resistance of the plant or direct disease suppression by antibiosis, com-
petition, or mycoparasitism (Ownley et al. 2010). The detailed host range of
B. bassiana has been shown in Table 10.1.
B. bassiana has been included in a schedule as an amendment in the Insecti-
cide Act, 1968, for commercial production as biopesticide and published in the
Gazette of India dated 26th March 1999 (Keswani et al. 2013). However, its
availability is still limited to some selected states in our country (Singh 2013).
The present chapter focuses on the crucial attributes of B. bassiana that are
responsible for its biocontrol activity. In addition, certain strain improvement
techniques have also been taken under consideration that would augment the in
situ action of the fungus. Besides, the heavy-metal remediation by B. bassiana
has also been discussed.
10 Beauveria bassiana: Biocontrol Beyond Lepidopteran Pests 221
10.1.1 Entomopathogenic vs. Endophytic Nature

of B. bassiana
B. bassiana is the most appreciated endophytic fungal entomopathogen to date,

with a widespread commercial availability as a potent mycopesticide. The fungus
establishes itself as an endophyte either naturally, e.g., by stomatal penetration, or
with the aid of inoculation methods such as soil drenches, seed coatings and
immersions, radicle dressings, root and rhizome immersions, stem injection, and
foliar and flower sprays. Hence, it is widely acknowledged as a success in a variety
of plants such as grasses; agricultural crops, viz., tomato, cotton, corn, and potato;
the medicinal group including opium poppy, cocoa, and coffee; and trees such as
Carpinus caroliniana and western white pine (Vega et al. 2008; Ownley
et al. 2008). The most preferred protocol for endophytic colonization of
B. bassiana includes the use of formulations augmented with solid inert carriers
or diluents such as diatomaceous earth, talc, clay, vermiculite, corn cob grits, and
alginate gels (Wagner and Lewis 2000; Parsa et al. 2013). Irrespective of the
multifarious inoculation techniques, the extent of colonization depends on the
type of plant part evaluated, the inoculation method used, and the initial spore
density on the plant tissue (Posada et al. 2007; Ownley et al. 2008). While leaves
respond best to foliar sprays, roots colonize only to drench inoculation with stems
responding equally to both forms. A plausible yet untested hypothesis suggests that
the extent of endophytic colonization correlates positively with the extent of
endophyte-mediated resistance by the host.
The insecticidal attribute of B. bassiana recommends its use in biopesticide
industry with particular reference to the malaria-causing mosquito vector. The
spores of B. bassiana have a high affinity for the female Anopheles mosquito.
However, for proper infestation, spore sufficiency and direct contact with the insect
is required so that the fungus can efficiently penetrate and germinate the insect
cuticle. Female Anopheles stephensi, the causal agent of human malaria in Asia,
relishes on the dead and dying caterpillars heavily infested with B. bassiana,
thereby letting themselves be infested in turn with this fungus. George
et al. (2013) premiered a caterpillar sans innovative technique of using
oil-formulated dried spores sprayed on a cloth that resulted in the fungal infestation
efficiency of 95 % in the mosquitoes. However, the utility of B. bassiana larvicide
can be good when we have regulated resistance in bacterial Bacillus sphaericus and
chemical larvicides.
10.2 Formulation Types of B. bassiana
Novel isolates of B. bassiana have been recommended for developing an efficient

bioformulation of biopesticide and mycopesticide. Primarily, three basic types of
formulations have been proposed for B. bassiana.
10.2.1 Conidia Mixed with Metabolites
Eyal (1993) developed a novel formulation using a saprophytic isolate producing

oosporin in high yield and evaluated its efficacy as biopesticide. This
bioformulation comprising of the fungal conidia (the biologically active form of
the fungus) treated with oosporin (produced by submerged culture) provides an
effective pest control measure particularly against Aphididae, Delphacidae,
Cicadellidae, Cercopidae, Aleyrodidae, Coccidae, Coleoptera, and Lepidoptera.
Moreover, it also showed potent application against mealybugs, spider mites, and
other foliar insects. The mode of application involves prilling of the mycelia as it
assures the retention of the biological activity of the product until application. At
the time of application, the dried prill is reactivated post-wetting and further used
for treating soil, seed, root, or plant (Eyal et al. 1994). In addition, the amendment
with food attractants or vegetable oils rich in oleic, linoleic, and linolenic acids in
bioformulation has proved a robust application in stimulation of necrophagy (Jack-
son et al. 2010).
10.2.2 Extracted Protein
An alternative mode of application suggests the use of finely emulated beauverial

protein extract, weighing about 5 kDa, either as granule, wettable powder, or dust
combined with inert materials, such as inorganic or botanical, or in liquid form such
as aerosol, foam, gel, suspension, or emulsifiable concentrates. The suggested
protein content in the bioformulation ranges from 1 to 95 % of the total weight of
the pesticide in dry form, while the liquid formulation consists of 1–60 % of the
total weight of solids in liquid phase (Leckie and Stewart 2006).
10.2.3 Endophytic Beauveria
Another novel isolate displaying endophytic colonization, reported by Vidal and

Tefera (2011), is much cashed upon for commercialization purpose. The said
isolate not only enjoys a broad prey range, including root weevils, wireworms,
maggots, bugs, aphids, beetles, soil grubs, root maggots, termites, and ants, but also
inhabits a diverse range of hosts, such as the vegetable crops and the cash crops.
The added advantage of using endophytic isolates is that they posses tolerance
against environmental stresses, such as UV, high temperature, rainfall, etc. The
fungal strain grows along with the host, and its additional entomopathogenic quality
renders a substantial lifelong protection to the host plants, particularly to agriculture
crops. The mode of incorporation involves dispersion, spray, gel, emulsion, layer,
cream, coating, dip, encapsulation, or granule. Moreover, they may also be incor-
porated as conventional microparticles or microcapsules.
10.3 Bioremediation of Heavy Metals: Potential Capacity

of B. bassiana in the Biosorption of Heavy Metals
Heavy metals render a cumulative harm to the living system by denaturation of

enzymes and proteins, production of ROS, displacement of essential metal ions
from biomolecules leading to conformational changes, and damage of membrane
integrity. Fungi are comparatively more tolerant to heavy-metal stress and thrive
well in the acidified environment owing to their adaptation of several strategies of
rendering the metal ions into innocuous forms. Hence, their use in bioremediation
of industrial effluents and wastewaters containing heavy metals has been reported
globally (Rajapaksha et al. 2004). The main principle for bioremediation is
biosorption that primarily utilizes amino, carboxyl, hydroxyl, and carbonyl groups
of the cell wall for metal binding as elucidated by FTIR analysis. A plethora of
fungal species, such as Aspergillus niger, Mucor rouxii, Rhizopus spp., etc., have
been reported to be utilized in heavy-metal remediation. Besides this, B. bassiana is
reported to efficiently adsorb Cd and Pb from aqueous metal solutions. Physico-
chemical factors like solution pH and contact time at room temperature positively
affected the rate of metal biosorption (Tomko et al. 2006).
Another strategy of heavy-metal uptake by fungal species involves immobiliza-
tion followed by precipitation. Fungal metal leaching is promoted by proton efflux
or metabolites with chelating properties. Metal immobilization can also occur
through other processes of reductive metal precipitation such as synthesis of
metallic nanoparticles that suggests its novel applications in the corrosion control
of metal and stone artifacts. B. bassiana is reported to be endowed with the ability
of heavy-metal chelation using oxalates as organic chelators. The beauverial
oxalate reportedly chelates a variety of metals, involving iron (iron oxalates were
directly sequestered on the fungal hyphae), copper (formation of characteristic
“Liesegang rings” due to simultaneous diffusion and precipitation of copper oxa-
lates), and silver (coprecipitation of copper and silver oxalates) (Joseph et al. 2012).
10.4 Secondary Metabolites of B. bassiana: A Boon

in Disguise
B. bassiana produces a plethora of secondary metabolites having multifarious roles

not only in pest control but in other human benefits too (Fig. 10.1). Some of the
essential secondary metabolites of this fungus have been discussed below.
Fig. 10.1 Multifarious roles of various secondary metabolites of B. bassiana
10.4.1 Bassiacridin
B. bassiana is reported to secrete a protein, bassiacridin, exhibiting toxicity for

locusts and comprising of about 0.1–0.3 % of the total protein content of the
crude extract. The protein exhibits a monomeric structure with a molecular
weight of 60 kDa and an isoelectric point of 9.5. It shows an amino acid sequence
homology to the yeast chitin-binding protein, but its chitin-binding properties
have not yet been determined. Being neutral for ion exchangers, bassiacridin
actively participates in β-glucosidase, β-galactosidase, and N-acetylglucosa-
minidase activities. Fourth-instar nymphs of Locusta migratoria injected with
the protein even at low dosage expressed a mortality rate of about 50 %. The
root cause of mortality as revealed by the ultrastructural studies showed melanin
pigmentation in the trachea, air sacs and a formation of melanized nodules on the
fat bodies leading to an alteration of the epithelial cell structure of the trachea,
air-bags, and integuments, thereby turning them necrotic. However, these struc-
tural changes were not reported to be caused by hemolymph pigmentation with
the simultaneous production of toxic quinones. The pigmentation activity was
carried out by the enzyme prophenoloxidase, activated by bassiacridin. Being
novel, research needs to be targeted on the understanding of the mechanism of
interaction of bassiacridin with host cells and its impact on the environment so as
to include the protein as an active ingredient of biopesticides (Quesada-Moraga
and Vey 2004).
10.4.2 Beauvericin
First discovered by Hamill et al. (1969), beauvericin is a cyclo-oligomeric

hexadepsipeptidic ionophore produced by B. bassiana with a widely acknowledged
insecticidal activity. The peptide binds to monovalent cations and facilitates their
transport across the membrane, thereby uncoupling oxidative phosphorylation
(Xu et al. 2008). Irrespective of its strong insecticidal potential, the preferential
use of a potent beauvericin-producing strain as a commercial insecticide is far more
advantageous than the pure compound primarily because its biosynthesis coincides
with infection. During the pathogenesis phase, the developing hyphae of
B. bassiana release extracellular hydrolytic enzymes aiding the fungal penetration
of insect integument (Fan et al. 2007) and virulence factors that disables and
crumbles the host immune system leading to its death. The activity of pure
beauvericin in entomopathogenesis is still controversial as it was reported to be
well tolerated by Helicoverpa zea.
Besides its role as an active insecticide, beauvericin displays a wide range of
in vitro biological activities, such as antibiotic, antiviral, and cytotoxic, and an
augmented antifungal activity when annexed with other antifungal agents in con-
sortia, as mentioned below.
10.4.2.1 Antitumor Activity
The cytotoxicity of beauvericin to a human leukemia cell has been frequently

reported. The peptide activates a calcium-sensitive cell apoptotic pathway.
Beauvericin induces Ca2+ ion transport from the extracellular environment to the
cytosol resulting in an augmentation of cytosolic Ca2+ concentration, thereby
triggering the onset of an “unknown signal system” with the subsequent release
of cyt c from mitochondria. The cyt c further activates the caspase inducing
apoptosis (Wang and Xu 2012). Beauvericin also inhibits directional cell motility
(haptotaxis) of cancer cells at sub-cytotoxic concentrations (Zhan et al. 2007)
10.4.2.2 Antibacterial Activity
Beauvericin displays a strong antibacterial activity against human, animal, and

plant pathogenic bacteria with no discrimination between Gram-positive and
Gram-negative bacteria. The target of action of beauvericin, contrary to other
fungal antibiotics, does not involve the peptidoglycan cell wall biosynthesis, but
the cell organelles and enzyme system of the bacteria. Based on its broad-spectrum
antibacterial activity, beauvericin could be used to figure out the solution to drug
resistance, deadly bacterial infections, and nonfood crop disease (Wang
et al. 2012).
10.4.2.3 Antifungal Activity
Beauvericin, being a fungal metabolite, displays no activity as an antifungal agent.

However, when used in combination with another compound, a whole new way of
development and utilization of the biological activity of beauvericin is unraveled,
e.g., beauvericin with ketoconazole in combination acts against Candida
parapsilosis, one of the major culprits of neonatal mortality, while both the
compounds, when applied singly, have little or no effect on this deadly fungus
(Zhang et al. 2007).
10.4.2.4 Antiviral Activity
The antiviral activity of beauvericin has also been detected. Shin et al. (2009)
proposed beauvericin as the most effective inhibitor of cyclic hexadepsipeptides
that inhibit HIV-1 integrase. However, an effective focus on the antiviral potential
of beauvericin is required for the exploration of its activity against the more fatal
and epidemic disease-causing viruses, such as HBV, SARS, H1N1, and AIV (Wang
et al. 2012).
10.4.3 Bassianolide
Another secondary metabolite of B. bassiana with insecticidal property is

bassianolide, a cyclo-oligomer depsipeptide (Xu et al. 2009). Comparative
sequence analysis of bassianolide synthetase (BbBSLS) suggested the presence of
catalytic domains intermediate from D-2-hydroxyisovalerate and L-leucine, respon-
sible for the iterative synthesis of dipeptidol monomer, D-hydroxyisovalerate
(D-Hiv) N-methyl-L-leucine (N-Me-Leu) bound to the enzyme. Post synthesis,
these domains catalyze the condensation of the monomers to form the
octadepsipeptide with a 24-membered macrolactone ring. This structure further
isomerizes to the final cyclic tetrameric ester form (Suzuki et al. 1977). Compar-
ative in vitro infection studies against selected insect host establish bassianolide as
a highly significant virulence factor of B. bassiana, inducing atony to the larvae of
H. zea and toxicity to silkworm larvae. Knockout mutants with a targeted disruption
of bbBsls gene were unable to produce bassianolide and showed a drastic reduction
in virulence against the model insects Galleria mellonella and H. zea. However,
B. bassiana orchestrates a definite strategy for carrying out the killing operation of
insect larvae in which the metabolite production coincides with the host infection.
Thus, bassianolide represents a bona fide virulence factor of the entomopathogen
significantly contributing to the commercial biological insecticide preparation
containing the spores of the fungus. Besides being a potent insecticide, purified
bassianolide inhibits the acetylcholine-induced smooth muscle contraction, as well
as displays in vitro cytotoxic, moderate antiplasmodial and antimycobacterial

activities (Jirakkakul et al. 2008).
10.4.4 Bassianolone
Ackland et al. (1985) isolated two rare metabolites, cephalosporolides E and F, as a

constituent of the fermentation products of the fungus, Cephalosporium aphidicola,
growing under sulfur-limiting conditions. Soon after this serendipitous discovery,
the same process could not be mimicked in laboratory conditions nor could these
metabolites be isolated from elsewhere in nature. However, Oller-Lopez
et al. (2005) unexpectedly found cephalosporolides E and F together with
bassianolone, an antimicrobial precursor of cephalosporolides E and F from
B. bassiana growing under low-nitrogen conditions. Though the in vitro antimi-
crobial activity of cephalosporolides E and F was completely negative,
bassianolone contradicted them by displaying a high antimicrobial activity with a
complete inhibition of the growth of Staphylococcus aureus and Candida albicans.
10.5 Internal Machinery that Renders the Fungal

Bioformulation a Success
10.5.1 Superoxide Dismutase
The basic criteria that determine the credibility of success of a particular fungal
formulation are its virulence and its tolerance to environmental stresses that dras-
tically affect the field efficacy and persistence of fungal sprays. Stress, in any form,
be it UV, heat, or drought, induces the production of reactive oxygen species (ROS)
which damages the essential biomolecules, like DNA, proteins, and lipids. Fungal
enzymes, particularly superoxide dismutases (SODs) capable of scavenging the
ROS, establish their virulence and field persistence. SODs are superoxide species
scavenging metalloproteins that offer the first line of defense against superoxide
damage. The SODs function in cell response, cell differentiation and infection
yielded the benefit of mycelial growth, ordered conidiation rhythm, and increased
sporulation potential and timely conidial germination in fungi. Fungal SODs are
Cu/Zn factored and existent mainly in cytosol or mitochondria (Mn). Mn-SOD has
proven to participate in the survival and resistance of aerobic fungi to multiple
stresses. A new Mn-SOD has been identified from B. bassiana that offers multi-
faceted attributes to the entomopathogen, including amplification of the SOD
activity by 4–10-fold, virulence to Spodoptera litura, and tolerance to chemical
oxidation and UV-B irradiation (Xie et al. 2010).
Two isomeric forms of BbSOD, BbSOD2 and BbSOD3, characterized as cyto-

solic and mitochondrial isoenzymes, dominated the total SOD activity in
B. bassiana under normal growth conditions. The biocontrol and stress retention
potential of the isoenzymes was determined by constructing knockout (DBbSOD2
and DBbSOD3) and RNAi mutants with a 91–97 % suppression of the BbSOD2 and
BbSOD3 transcripts. The constructs were used in conjunction with DBbSOD2/
BbSOD2, DBbSOD3/BbSOD3, and wild type. Both the mutant types displayed
marked phenotypic alterations, such as delayed sporulation, reduced conidial yield,
and impaired conidial quality, but little change in colony morphology. The mycelia
and conidia expressed greater sensitivity to menadione- or H2O2-induced oxidative
stress but little or no response to hyperosmolarity or elevated temperature, in
contrast to the yeast mitochondrial Mn-SOD which protected the yeast cells from
osmotic, oxidative, and thermal stress. While disruption of either of the genes
resulted in reduced conidial yield and delayed germination, the double-gene-
silenced mutants expressed defective sporulation, increased sensitivity to menadi-
one and H2O2, a longer delay in conidial germination, and a decreased virulence,
due to a greater loss of the antioxidative capability leading to an increased level of
intracellular superoxide species. This fact suggests the additive influence exertion
of the two Mn-SODs mediating fungal development, antioxidative capacity, UV
resistance, and virulence. However, the increased tolerance of mycelia and conidia
of DBbSOD2 to the two oxidants than DBbSOD3 suggested the greater contribu-
tion of the mitochondrial BbSOD3 to the fungal antioxidative capability.
10.5.2 Hydrophobins
The entomopathogenic B. bassiana, existing both as a saprobe and endophyte,

comprises of several infectious propagules, such as conidia (aerial and submerged),
in vitro unicellular blastospores, and in vivo insect hemolymph derived cells,
hyphal bodies. The process of pathogenesis commences with the adhesive interac-
tion between the conidial surface and the insect epicuticle by nonspecific hydro-
phobic interactions, mediated by the spore-coat hydrophobins. B. bassiana
comprises two genes, hyd1 and hyd2, termed as the hydrophobin genes, responsible
for cell-surface hydrophobicity, adhesion, virulence, spore thermotolerance, and
formation of the rodlet layer, a protective spore-coat structure. Bioinformatic and
phylogenetic studies classified both the proteins as class I hydrophobins with no
primary sequence homology between them. N-terminal amino acid sequencing of a
rodlet layer protein suggested Hyd2 as a spore-coat component. Deactivation of
hyd1, i.e., dhyd1, displayed characteristic phenotypic alterations such as apparently
“bald” conidia with modified surface fascicles or bundles. The spores were hydro-
phobic with different surface carbohydrate epitopes and β-1,3 glucan distribution.
Further, their mode of dispersal was sans water, and their virulence was also
lowered, but adhesion was not affected. On the contrary, adhesion was lowered in
Dhyd2, but the mutants were unaffected in virulence. The double-gene-silenced
mutants expressed a lack of bundles and rodlets and a drastically lower surface
hydrophobicity, cell attachment, and virulence (Ying and Feng 2004; Zhang
et al. 2011).
Apart from being a spore surface component, hydrophobins play a characteristic
role in the physiology of the organism. The hydrophobin acts as a regulator that
senses the environmental conditions, and if found suitable, the spore is allowed to
germinate. Thus, at an elevated temperature, hyphal growth would be
compromised, and conidial germination inhibition would be adaptive. The above
explanation was derived from a distinctly impaired adhesion phenotype in single-
and double-gene knockout mutants. Dhyd1 mutant displayed a drastic reduction in
virulence when topically assayed against G. mellonella larvae as compared to
Dhyd2 mutants though the latter one showed a prominent reduction in adhesion.
The intrigue could be explained by the probable clumping of the Dhyd1 conidia
resulting in a decreased germination and virulence. In conclusion, hyd1 and hyd2
encode class I hydrophobins that are essential for spore-coat rodlet layer and
fascicle formation. Besides, Hyd1 forms the rodlet with Hyd2 acting as an organiz-
ing partner (Zhang et al. 2011).
10.5.3 Primary Roles of Two Dehydrogenases

in the Mannitol Metabolism and Multistress
Tolerance of B. bassiana
Mannitol metabolism in B. bassiana corresponds with multistress tolerance and

virulence. This attribute is of prime importance where a constant challenge from the
various stress factors, such as UV and high temperature, is faced and, particularly in
the case of the fungal bioformulation, exposed to the environment. B. bassiana
hosts two enzymes, namely, mannitol-1-phosphate dehydrogenase (MPD) for
reducing fructose-6-phosphate to mannitol-1-phosphate and mannitol dehydroge-
nase (MTD) for oxidizing mannitol to fructose. Intracellular mannitol accumulation
relates to the increased conidial tolerance to various stresses. This fact was
evidenced in single knockout mutants DBbMPD and DBbMTD where a drastic
abatement in the mannitol content resulted in a steep decline in conidial tolerance to
the various stresses as well as virulence ability as observed against S. litura larvae.
Also, mannitol-supplemented nitrate-based minimal medium suppressed the colony
growth and conidial germination of DBbMTD at a larger level as compared to
DBbMPD (Wang et al. 2012). However, mannitol content decline was supported by
trehalose accumulation. Trehalose accumulation is regulated by the enzyme
trehalase. Trehalose acts as a carbohydrate store and offers a temporary respite
during the stress conditions (Liu et al. 2009). However, owing to its compartmen-
talization, trehalose cannot replace mannitol to increase conidial thermotolerance.
Conclusively, BbMPD and BbMTD represent the flag bearers of mannitol
metabolism in B. bassiana contributing to conidial thermotolerance, UV-B resis-

tance, and virulence.
10.5.4 MAP Kinase Activity
MAP kinases are widely described in eukaryotes, including fungi, and are known to
play essential roles in the transduction of extracellular environmental signals,
regulating development and differentiation process. Filamentous fungi harbor basi-
cally three classes of MAP kinases, Fus3/Kss1, Hog 1, and Slt2, with orthologs of
each of these kinases being present in B. bassiana. Fus3/Kss1 MAPK deals with the
regulation of infection-related development or process leading to the penetration of
host tissues. The Hog1 MAPK is known to be involved in the mediation of
virulence and responses against various environmental stresses (osmotic, oxidative,
and thermal) and fungicides. Slt2 MAPK is likely to be crucial for the fungal
survival in the environment. Bbslt2 controls growth, conidiation, cell wall integrity,
response to oxidative and heat stress, heterokaryon formation, secondary metabolic
pathways, and virulence in the entomopathogenic fungus. The above attributes
were antipodally expressed in the knockout mutant of DBbslt2, as reduction in
conidial production and viability, temperature-dependent chitin accumulation but
with a simultaneous chitinal sensitivity to Congo red and fungal cell wall-degrading
enzymes, and decreased conidial and hyphal hydrophobicity without alteration in
the hydrophobin-encoding genes, hyd 1 and hyd 2. Besides, the cell-surface carbo-
hydrate epitopes also expressed an alteration with a change in the content of acid-
soluble, alkali-insoluble, and β-glucans as well as an attenuation in virulence as
observed on topical and intra-hemocoel application. The content of trehalose was
also reported to be elevated in the mutated strains as a stabilizing factor during
stress conditions (Zhang et al. 2009; Luo et al. 2012).
10.5.5 Role of G-Protein Signaling (BbRGS1)

in Conidiation and Conidial Thermotolerance
of B. bassiana
The chief virulence attribute of the entomopathogenic fungus involves conidial

germination leading to pathogenesis and disease transmission. As in other fungi,
conidiation and germination in B. bassiana is regulated by regulatory G-protein
signaling RGS protein encoded by Bbrgs1. RGS functions in a G-protein-mediated
signaling pathway that responds to environmental signals by regulating the
switches between vegetative growth and conidiation. Activation of the gene results
in the initiation of vegetative growth and termination in conidiation, while gene
repression accelerates conidiation. However, the knockout mutant exhibiting the
complete loss of Bbrgs1 gene only reduced conidial production in contrast to other
fungi suggesting an alternative mechanism or other RGS proteins in addition to
BbRGS1 controlling conidiation in B. bassiana (Fang et al. 2008). Apart from
conidiation, Bbrgs1 is actively involved in toxin synthesis, pigment production,
stress tolerance, and thermotolerance. The ability to control conidiation and
thermotolerance in insect pathogenic fungus as well as the ability to control the
dissemination of genetically modified strains in field application has an important
implication for the mass production of this biocontrol agent.
10.6 Strain Improvement in B. bassiana
The entomopathogenic ability of B. bassiana has been extensively studied and

advocated in insect pest management (Roberts and St Leger 2004; Wang
et al. 2004; Thomas and Read 2007). However, a major hindrance toward its
commercialization and application is its slower action compared to chemical
insecticides, which enables the infected insects to cause a serious damage to
crops until controlled (St Leger et al. 1996). To overcome such obstacles, there
have been constant efforts for the improvement of the desired strains which render
their quick action and high reproducibility. Various approaches have been
employed to develop enhanced B. bassiana strains, including the recovery of
mutants after UV-light irradiation (Hegedus and Khachatourians 1995; Meirelles
et al. 1997). Other reported methods involve genetic recombination (Viaud
et al. 1998) and genetic constructs (Fan et al. 2007; Fang et al. 2005; Joshi
et al. 1995; Sandhu et al. 2001).
Genetic engineering, involving the identification and manipulation of the viru-
lent genes, has elevated the insecticidal efficacy and biocontrol potential of the
fungus. The expression of a neurotoxin AAIT from the scorpion Androctonus
australis and an insect cuticle-degrading protease PR1A from Metarhizium
anisopliae has permitted a high efficiency of B. bassiana. When assayed against
the larvae of Masson’s pine caterpillar Dendrolimus punctatus and the wax moth
G. mellonella, engineered strains required less spores to kill 50 % of pine caterpil-
lars (LD50) (Lu et al. 2008).
Insect cuticle is mainly composed of chitin, embedded with proteins and acts as a
primary barrier against pathogen attack. B. bassiana produces chitinases and pro-
teases to disintegrate insect cuticle. Two chitinases (Bbchit1 and Bbchit2) have
been reported in B. bassiana lacking a chitin-binding domain. However, hybrid
chitinases were developed in which Bbchit1 was fused to chitin-binding domains
derived from plant, bacterial, or insect sources. The hybrid chitinase gene was
transformed in B. bassiana, and the transformed strains confirmed higher levels of
virulence resulting in 23 % less time to kill the targeted insects (Fang et al. 2005).
Protoplast fusion may provide as a striking method for genetic improvement of
biocontrol efficacy of B. bassiana. Paris (1977) first described the construction of
parasexual heterokaryons through a protoplast fusion between two strains of
Beauveria tenella. Till date, many intraspecific and interspecific fusions have been
reported in genus Beauveria. The fusants obtained through protoplast fusion of
B. bassiana with B. sulfurescens have been reported to possess enhanced antago-
nistic activity against Colorado potato beetle (Leptinotarsa decemlineata) and
European corn borer (Ostrinia nubilalis) (Couteaudier et al. 1996).
T-DNA insertion mutagenesis is being used to identify and isolate the genes
governing thermotolerance and osmotolerance. A pool of T-DNA inserts of
B. bassiana have been constructed for detection of mutants deficient in
thermotolerance and osmotolerance ability. Five mutants were reported which
posses high conidial yield, virulence, and resistance to adverse conditions (Luo
et al. 2009).
10.7 Conclusion
B. bassiana is a profusely growing saprophytic as well as endophytic fungus.

However, many of its potentials remain unrealized till date. This chapter was a
small attempt to enhance the knowledge of some of the beneficial attributes of
B. bassiana. The bioformulations of this fungus will not only be realized in
pathogen control but will also augment the remediation of heavy metals. These
attributes of B. bassiana are in perfect synchronization with the environment and
when optimized will lead to surplus production, thereby reducing its price and
making the formulations readily available.
Acknowledgements Chetan Keswani and Shatrupa Ray are grateful to Banaras Hindu Univer-
sity, Varanasi, for providing the CRET-UGC fellowship.
References
Ackland MJ, Hanson JR, Hitchcock PB, Ratcliffe AH (1985) Structures of the cephalosporolides
B-F, a group of C, lactones from Cephalosporium aphidicola. J Chem Soc Perkin Trans 1:843–
847
Bruck DJ (2010) Fungal entomopathogens in the rhizosphere. In: The ecology of fungal
entomopathogens. Springer, Heidelberg, pp 103–112
Couteaudier Y, Viaud M, Riba G (1996) Genetic nature, stability, and improved virulence of
hybrids from protoplast fusion in Beauveria. Microb Ecol 32:1–10
Eyal J (1993) Novel toxin producing fungal pathogen and uses. European Patent No. 0570089A1.
European Patent Office
Eyal J, Landa Z, Osborne L, Walter JF (1994) Method for production and use of pathogenic fungal
preparation for pest control. U.S. Patent No. 5,360,607. U.S. Patent and Trademark Office,
Washington, DC
Fan Y, Fang W, Guo S, Pei X, Zhang Y, Xiao Y, Pei Y (2007) Increased insect virulence in
Beauveria bassiana strains overexpressing an engineered chitinase. Appl Environ Microbiol
73:295–302
Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Pei Y (2005) Cloning of Beauveria bassiana
chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ
Fang W, Scully LR, Zhang L, Pei Y, Bidochka MJ (2008) Implication of a regulator of G protein
signalling (BbRGS1) in conidiation and conidial thermotolerance of the insect pathogenic
fungus Beauveria bassiana. FEMS Microbiol Lett 279:146–156
George J, Jenkins NE, Blanford S, Thomas MB, Baker TC (2013) Malaria mosquitoes attracted by
fatal fungus. PLoS One 8:e62632
Gurulingappa P, Sword GA, Murdoch G, McGee PA (2010) Colonization of crop plants by fungal
entomopathogens and their effects on two insect pests when in planta. Biol Control 55:34–41
Hamill RL, Higgens CE, Boaz HE, Gorman M (1969) The structure of beauvericin, a new
depsipeptide antibiotic toxic to Artemia salina. Tetrahedron Lett 49:4255–4258
Hegedus DD, Khachatourians GG (1995) The impact of biotechnology on hyphomycetous fungal
insect biocontrol agents. Biotechnol Adv 13:455–490
Jackson MA, Dunlap CA, Jaronski ST (2010) Ecological considerations in producing and formu-
lating fungal entomopathogens for use in insect biocontrol. In: The ecology of fungal
entomopathogens. Springer, Heidelberg, pp 129–145
Jirakkakul J, Punya J, Pongpattanakitshote S, Paungmoung P, Vorapreeda N, Tachaleat A,
Cheevadhanarak S (2008) Identification of the nonribosomal peptide synthetase gene respon-
sible for bassianolide synthesis in wood-decaying fungus Xylaria sp. BCC1067. Microbiology
154:995–1006
Joseph E, Cario S, Simon A, Wörle M, Mazzeo R, Junier P, Job D (2012) Protection of metal
artifacts with the formation of metal–oxalates complexes by Beauveria bassiana. Front
Microbiol 2:270
Joshi L, St Leger RJ, Bidochka MJ (1995) Cloning of a cuticle‐degrading protease from the
entomopathogenic fungus, Beauveria bassiana. FEMS Microbiol Lett 125:211–217
Keswani C, Singh SP, Singh HB (2013) Beauveria bassiana: status, mode of action, applications
and safety issues. Biotech Today 3:16–20
Leckie B, Stewart C (2006) Insecticidal compositions and methods of using the same. U.S. Patent
20070044179 A1
Liu Q, Ying SH, Feng MG, Jiang XH (2009) Physiological implication of intracellular trehalose
and mannitol changes in response of entomopathogenic fungus Beauveria bassiana to thermal
stress. Anton Leeuw Int J G 95:65–75
Lu D, Pava-Ripoll M, Li Z, Wang C (2008) Insecticidal evaluation of Beauveria bassiana
engineered to express a scorpion neurotoxin and a cuticle degrading protease. Appl Microbiol
Luo Z, Zhang Y, Jin K, Ma J, Wang X, Pei Y (2009) Construction of Beauveria bassiana T-DNA
insertion mutant collections and identification of thermosensitive and osmosensitive mutants.
Acta Microbiol Sin 49:1301–1305
Luo X, Keyhani NO, Yu X, He Z, Luo Z, Pei Y, Zhang Y (2012) The MAP kinase Bbslt2 controls
growth, conidiation, cell wall integrity, and virulence in the insect pathogenic fungus
Beauveria bassiana. Fungal Genet Biol 49:544–555
Meirelles LDP, Boas AMV, Azevedo JL (1997) Obtention and evaluation of pathogenicity of ultra
violet resistant mutants in the entomopathogenic fungus Beauveria bassiana. Rev Microbiol
28:121–124
Oller-Lopez JL, Iranzo M, Mormeneo S, Oliver E, Cuerva JM, Oltra JE (2005) Bassianolone: an
antimicrobial precursor of cephalosporolides E and F from the entomoparasitic fungus
Beauveria bassiana. Org Biomol Chem 3:1172–1173
Ownley BH, Griffin MR, Klingeman WE, Gwinn KD, Moulton JK, Pereira RM (2008) Beauveria
bassiana: endophytic colonization and plant disease control. J Invertebr Pathol 98:267–270
Ownley BH, Gwinn KD, Vega FE (2010) Endophytic fungal entomopathogens with activity
against plant pathogens: ecology and evolution. In: The ecology of fungal entomopathogens.
Springer, Heidelberg, pp 113–128
Paris S (1977) Heterocaryons chez Beauveria tenella. Mycopathologia 61:67–75

Parsa S, Ortiz V, Vega FE (2013) Establishing fungal entomopathogens as endophytes: towards
endophytic biological control. J Vis Exp: JoVE 74:50360
Posada F, Aime MC, Peterson SW, Rehner SA, Vega FE (2007) Inoculation of coffee plants with
the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales). Mycol Res
111:748–757
Quesada-Moraga E, Vey A (2004) Bassiacridin, a protein toxic for locusts secreted by the
entomopathogenic fungus Beauveria bassiana. Mycol Res 108:441–452
Rajapaksha RMCP, Tobor-Kapłon MA, Baath E (2004) Metal toxicity affects fungal and bacterial
activities in soil differently. Appl Environ Microbiol 70:2966–2973
Roberts DW, St Leger RJ (2004) Metarhizium spp., cosmopolitan insect-pathogenic fungi: myco-
logical aspects. Adv Appl Microbiol 54:31–36
Sandhu SS, Kinghorn JR, Rajak RC, Unkles SE (2001) Transformation system of Beauveria
bassiana and Metarhizium anisopliae using nitrate reductase gene of Aspergillus nidulans.
Indian J Exp Biol 39:650–653
Shin CG, An DG, Song HH, Lee C (2009) Beauvericin and enniatins H, I and MK1688 are new
potent inhibitors of human immunodeficiency virus type-1 integrase. J Antibiot 62:687–690
Singh HB (2013) Beauveria bassiana in Indian agriculture: perception, demand and promotion. In:
10th international conference of insect physiology, biochemistry and molecular biology,
Nanjing, 15–19 June 2013, p 122
St Leger RJ, Joshi L, Bidochka MJ, Roberts DW (1996) Construction of an improved
mycoinsecticide overexpressing a toxic protease. Proc Natl Acad Sci USA 93:6349–6354
Suzuki A, Kanaoka M, Isogai A, Tamura S, Murakoshi S, Ichinoe M (1977) Bassianolide, a new
Lett 18:2167–2170
Thomas MB, Read AF (2007) Can fungal biopesticides control malaria? Nat Rev Microbiol
5:377–383
Tomko J, Bačkor M, Štofko M (2006) Biosorption of heavy metals by dry fungi biomass. Acta
Metallurgica Slovaca 12:447–451
Vega FE, Posada F, Catherine Aime M, Pava-Ripoll M, Infante F, Rehner SA (2008)
Entomopathogenic fungal endophytes. Biol Control 46:72–82
Viaud M, Couteaudier Y, Riba G (1998) Molecular analysis of hypervirulent somatic hybrids of
the entomopathogenic fungi Beauveria bassiana and Beauveria sulfurescens. Appl Environ
Vidal S, Tefera T (2011) U.S. Patent Application 13/636 143
Wagner BL, Lewis LC (2000) Colonization of corn, Zea mays, by the entomopathogenic fungus
Beauveria bassiana. Appl Environ Microbiol 66:3468–3473
Molecules 17:2367–2377
Wang C, Fan M, Li Z, Butt TM (2004) Molecular monitoring and evaluation of the application of
the insect‐pathogenic fungus Beauveria bassiana in southeast China. J Appl Microbiol 96:861–
870
Wang ZL, Lu JD, Feng MG (2012) Primary roles of two dehydrogenases in the mannitol
metabolism and multi‐stress tolerance of entomopathogenic fungus Beauveria bassiana. Envi-
ron Microbiol 14:2139–2150
Xiao G, Ying SH, Zheng P, Wang ZL, Zhang S, Xie XQ, Feng MG (2012) Genomic perspectives
on the evolution of fungal entomopathogenicity in Beauveria bassiana. Sci Rep 2
Xie XQ, Wang J, Huang BF, Ying SH, Feng MG (2010) A new manganese superoxide dismutase
identified from Beauveria bassiana enhances virulence and stress tolerance when
overexpressed in the fungal pathogen. Appl Microbiol Biotechnol 86:1543–1553
Xu Y, Orozco R, Wijeratne EM, Gunatilaka AA, Stock SP, Molnár I (2008) Biosynthesis of the
cyclooligomer depsipeptide beauvericin, a virulence factor of the entomopathogenic fungus
Beauveria bassiana. Chem Biol 15:898–907
Xu Y, Orozco R, Kithsiri Wijeratne EM, Espinosa-Artiles P, Leslie Gunatilaka AA, Patricia

Stock S, Molnár I (2009) Biosynthesis of the cyclooligomer depsipeptide bassianolide, an
insecticidal virulence factor of Beauveria bassiana. Fungal Genet Biol 46:353–364
Ying SH, Feng MG (2004) Relationship between thermotolerance and hydrophobin‐like proteins
in aerial conidia of Beauveria bassiana and Paecilomyces fumosoroseus as fungal biocontrol
agents. J Appl Microbiol 97:323–331
Zhan J, Burns AM, Liu MX, Faeth SH, Gunatilaka AL (2007) Search for cell motility and
angiogenesis inhibitors with potential anticancer activity: beauvericin and other constituents
of two endophytic strains of Fusarium oxysporum. J Nat Prod 70:227–232
Zhang L, Yan K, Zhang Y, Huang R, Bian J, Zheng C, Chen X (2007) High-throughput synergy
screening identifies microbial metabolites as combination agents for the treatment of fungal
infections. Proc Natl Acad Sci USA 104:4606–4611
Zhang Y, Zhao J, Fang W, Zhang J, Luo Z, Zhang M, Pei Y (2009) Mitogen-activated protein
kinase hog1 in the entomopathogenic fungus Beauveria bassiana regulates environmental
stress responses and virulence to insects. Appl Environ Microbiol 75:3787–3795
Zhang S, Xia YX, Kim B, Keyhani NO (2011) Two hydrophobins are involved in fungal spore
coat rodlet layer assembly and each play distinct roles in surface interactions, development
and pathogenesis in the entomopathogenic fungus, Beauveria bassiana. Mol Microbiol
80:811–826
Chapter 11
Biocontrol of Diamondback Moth, Plutella
xylostella, with Beauveria bassiana and Its
Metabolites
Liande Wang, Minsheng You, and Haichuan Wang
11.1 Introduction
The development of resistance to chemical insecticides and concerns over the

deleterious effects of chemicals on environmental and human safety have provided
a strong impetus for the development of microbial control agents for use in
integrated control of insect pests. A diverse assemblage of microorganisms are
currently under consideration as control agents of insects, including viruses, bac-
teria, protozoans, and fungi. Fungi will not be cure-alls for pest problems on all
crops and in all agricultural settings, and it is unlikely that they will ever totally
supplant the management of insect pests with chemical insecticides. Nevertheless,
they represent a valuable management resource to be utilized within an IPM
framework and will contribute significantly to reductions in chemical pesticide
use (Lacey and Goettel 1995). However, the research development and final
commercialization of fungal biological control agents (BCAs) continue to confront
a number of obstacles, ranging from elucidating important basic biological knowl-
edge to socioeconomic factors (Butt et al. 2001). Currently, considerable advances
have been made in the infection mechanism (Wang et al. 2007, 2010). In this
chapter, we present the biocontrol of diamondback moth (DBM), Plutella xylostella
with Beauveria bassiana and its metabolites, with an elucidation on the infection
behavior of B. bassiana to P. xylostella and virulence of the fungal isolate and its
metabolites. The fungi that have received the majority of attention for P. xylostella
control are the Deuteromycetes because of their prevalence, possibility for produc-
tion on artificial media, ease of application, and relatively long shelf lives.
L. Wang (*) • M. You

Institute of Applied Ecology, Key Laboratory of Biopesticide and Chemical Biology, MOE,
Fujian Agriculture and Forestry University, Fuzhou 350002, China
H. Wang
University of Nebraska-Lincoln, 312 Entomology Hall, Lincoln, NE 68583, USA

DOI 10.1007/978-3-319-14499-3_11
238 L. Wang et al.
11.2 Virulence of Different B. bassiana Strains

to P. xylostella
Wang (1999) bioassayed four strains of B. bassiana (Bb-100, Bb-71, Bb62, and
Bb-38, conidial spore suspension 3 107 conidia/ml) to P. xylostella (second and
third instars) in both laboratory and a small-scale field (in the campus of Fujian
Agriculture and Forestry University, Fuzhou, PR China). As observed in Figs. 11.1
and 11.2, larvae became infected as they were inoculated. A time-mortality rela-
tionship was seen to exist. In the field and laboratory experiments, Bb-100 showed
the strongest ability to kill the insect host. Due to imprecise mortality estimates in
the field, it was difficult to quantify the effect of LT50. The LT50 to DBM with
a
90
80
70
60
Mortality (%)
50
40
30
20
10
Days
b
70
60
50
Mortality (%)
40
30
20
10
Days
Fig. 11.1 Virulence assay of four strains of B. bassiana against P. xylostella in (a) laboratory, (b)
field (cf. Wang 1999; You et al. 2004)
11 Biocontrol of Diamondback Moth, Plutella xylostella, with Beauveria bassiana. . . 239
90
80
70
60
Mortality (%)
50
40
30
20
10
Days
Fig. 11.2 Virulence assay of strain Bb-100 of B. bassiana against P. xylostella at different
temperatures (cf. Wang 1999; You et al. 2004)
Bb-100 was 8.5 days in the laboratory; the starting time was over 5 days in the
laboratory and field. Among the four strains, Bb-38 exhibited the least virulence to
DBM.
To reveal environmental effect on the virulence of B. bassiana, a simple matrix
treatment considering P. xylostella and Bb-100 was carried out in the laboratory.
The infectious ability of Bb-100 strongly varied with temperature. Figure 11.2
indicates that the mortality approximately began 4–5 days after application. The
highest level of mortality was seen at 28 C, but the fastest time to knock down the
host was at 32 C. Low temperature delayed the activity in infection. On the other
hand, it was reported that relative humidity could influence the mortality of
P. xylostella (Masuda 1998). Undoubtedly, temperature is one of the basic factors
of virulence of B. bassiana.
11.3 Infection Behavior of B. bassiana to P. xylostella
11.3.1 Infection Process
The process of fungal infection involves four steps: adhesion, germination, differ-
entiation, and penetration (St. Leger et al. 1996).
240 L. Wang et al.
11.3.1.1 Attachment
Attachment of fungal spores to the host is a prerequisite for further parasitic events
and takes place together with host recognition. Conidial attachment is one of the
dominant steps in the course of infection of entomopathogenic fungi. This exper-
iment focused on the initial behavior of the conidia of B. bassiana on the cuticle of
the third instar of P. xylostella. With the infection process used, conidia were
commonly found on the ventral side, in segmental vicinity, and on no-sticklike
area on the host. Clearly, the conidia (1.8–2.8 μm) are almost as large as the size of
space among hairs on the cuticle surface of P. xylostella; therefore, conidia were
getting a full touch with P. xylostella cuticle. Despite this, there were still some
conidia which contacted larval epicuticle at rare hairy region (e.g., intertegumental
membrane, abdomen, etc.), germinated, and breached the epicuticle (Figs. 11.3 and
11.4). The increasing evidence of conidial germination was clearly presented after
24 h. The surface of conidia possesses an outer layer of interwoven pins (Figs. 11.5
and 11.6). The structure is unique to the conidial stage, not detected on the
vegetative cells (hyphal body, mycelium). The interwoven pins were arranged in
groups, mainly in a vertical position, but near the top, there were several pins
positioned horizontally (Fig. 11.6, arrows; Wang 1999).
This structure was rarely observed, only in conidia submerged in liquid media,
and not those on dry. The superficial structure on a conidium was as similar as
indicated in Fig. 11.6. The existence of such structures suggests that the topography
of the conidium assists the recognition or attachment between conidium and host
(Zhai and Huang 1995; Bidochka et al. 1993). After coming in contact with the
cuticle of both hosts, conidium began to release some mucilage materials around
the conidium (Fig. 11.7a). Obviously, at the contact point or nearby, materials cover
the outer layer of conidia, which may serve as glue (Large et al. 1988). Compared
with other parts of the conidium, the mucus around the part touching the cuticle was
thicker. After germination, mucilage covered the outer conidium wall, but at the top
Fig. 11.3 Superficial

epicuticle of P. xylostella
infected by Bb-100 under
SEM, conidia (C), germ
tube (GT), hairs
(HS) (cf. Wang 1999;
You et al. 2004)
Fig. 11.4 Cross section of

the cuticle of P. xylostella
under SEM, hairs
(HS) (cf. Wang 1999; You
et al. 2004)
Fig. 11.5 TEM of a conidium, conidium (C), conidial wall (CW), vacuole (V), nucleus (N),
floccule (F) (cf. Wang 1999; You et al. 2004)
of the germ tube, the mucilage was much thicker than other parts (Fig. 11.7b, c
under TEM). At this stage, no septum was observed between the germ tube and
maternal conidium. Underneath, comparing with the parts nearby, the cuticle of
P. xylostella was turned to gray in color, showing a distinct deformation of the
epicuticle. It is suggested that within either part of the germ tube or contacting part
of the conidium with the cuticle would be the most metabolically active section; the
242 L. Wang et al.
Fig. 11.6 (a) A view of the conidium under TEM, conidium (C), rodlet (R); (b) a general view of
the conidium under TEM, conidium (C), rodlet-like structure (arrow) (cf. Wang 1999; You
et al. 2004)
Fig. 11.7 Conidium on the cuticle of P. xylostella under TEM, conidium or conidia (C), mucilage
(M), epicuticle (E), germ tube (GT); (a) conidium falling down on cuticle of P. xylostella, arrow
indicating degraded epicuticle, bar ¼ 500 nm; (b and c) germinating conidia on the epicuticle of
P. xylostella, bar ¼ 100 nm (cf. Wang 1999; You et al. 2004)
mucilage outside was secreted by the conidium and germ tube themselves (Wang
1999).
11.3.1.2 Germination
The germination tests proceeded after inoculation on different hosts in the labora-
tory (Table 11.1). Even within a given laboratory, the germination ratio varied with
Table 11.1 Assay of conidial germination and appressorial formation on different media
Rate (%)/+0.0125 YEM Rate (%)/+sterilized water
Strain Item 12 h 24 h 36 h 12 h 24 h 36 h
Bb-100 GC 3.5 18 25 2.0 6.6 20
AP 1.2 3.0 5.0 1.0 2.1 3.0
Bb-62 GC 2.2 5.6 5.6 1.0 3.8 6
AP 0.5 1.7 1.7 0.1 1.6 1.6
Bb-71 GC 1.0 4.0 5.2 0.0 4.0 4.1
AP 0.0 1.8 2.7 0.0 2.0 2.5
Bb-38 GC 1.5 26 30 1.0 25 30
AP 0.4 11 19 0.0 14 19
Suspension 3 107 conidia/ml + 0.0125 YEM, 28 C pH ¼ 6.5 or suspension 3 107 conidia/ml
+ sterilized water, 28 C pH ¼ 6.5, germinating conidia (GC), appressoria (AP), cf. Wang (1999)
and You et al. (2004)
the strain and host. Clearly, the conidia could germinate on all hosts, after 24 h.
With the elongation of the incubation time, the number of germinated conidia
(GC) on all hosts increased linearly. In treatments adding 1.25 % YEM, the
germination ratio was higher than those with only sterilized water. The result that
suitable addition of nutrient could stimulate the germination of conidia was con-
sistent with Fan and Li (1994) and St. Leger et al. (1991, 1989). Bb-38 could easily
germinate on P. xylostella (Wang 1999).
Under the conditions of 28 C, pH ¼ 6, 80 % RH, in the four strains of
B. bassiana, the amount of conidial germination increased with time. With the
infection process used, the GC could be found at any part of the cuticle including
the head, thorax, and elytra under SEM (Fig. 11.8).
On cuticle, although some conidia only contacted with horn-like nodules, they
still appeared in taxis bending toward the cuticle (Fig. 11.8, under SEM). The germ
tube of Bb-100 on the abdomen of P. xylostella (Fig. 11.8V) had less orientation,
but on the head and other parts (Figs. 11.7 and 11.8I, II), Bb-71 showed a little taxis
(Fig. 11.8III, IV). Taking the materials that exist on the epicuticle into consider-
ation, differences in conidial orientation are probably due to components that can
have prohibiting or stimulating functions on conidia (Zhai and Huang 1997;
St. Leger et al. 1994). Before penetrating into P. xylostella, the germ tubes were
2.2–6.6 μm in length in Bb-100 and Bb-62. In Bb-71, germ tube length was around
11.2 μm. Extensive amounts of errant-growing hyphae were rare to see, but limited
amounts of errant growth followed by eventual penetration over the surface were
seen (Fig. 11.8VI, VII for strain Bb-100 on P. xylostella). At early stage about 24 h,
few big vacuoles filled the whole conidium; the bud of the germ tube formed at
random, into which the vacuole expanded (Fig. 11.8VIII for strain Bb-100). After
48 h, nuclear division was finished, materials in the conidium were transmitted
toward the germ tube, a big vacuole was formed consequently, and the top of germ
tubes sometimes tended to be thin (Fig. 11.8IX for strain Bb-100). In late stages, the
septum was formed between the conidium and germ tube, and daughter nuclei
moved into the germ tube (Fig. 11.8X) (Wang 1999).
244 L. Wang et al.
Fig. 11.8 (I ) Superficial view of the thorax of P. xylostella infected with Bb-100 after 24 h under
SEM, scale bar ¼ 1 μm; (II) fine structure of the head of P. xylostella contaminated with Bb-100
after 24 h under SEM, germinating conidia (arrow), scale bar ¼ 1 μm; (III) abdomen of
P. xylostella contaminated by Bb-71 after over 36 h under SEM, hyphae penetrating into epicuticle
11.3.1.3 Appressoria
The second instar of P. xylostella was contaminated by four strains and provided a
rich form of appressoria and appressoria-like structures. In Figs. 11.8IV, VII and
11.9I, II, a penetration peg for host penetration was produced, but the penetration
peg was formed at a position near to germ tube in Fig. 11.9II, while it came into
being at the tip of germ tube as in Figs. 11.8VII and 11.9I, II. Nearly all penetration
pegs breached the cuticle. Enzyme activity was presented at the tip of the penetra-
tion peg as in Fig. 11.9II. Appressoria of B. bassiana were usually formed at the end
of a germ tube of variable lengths and often terminated in a bulb from which a
narrow hypha (penetration peg) emerged. Appressoria were occasionally formed
apically on short germ tubes or directly from conidia (Fig. 11.9I, II). Evidence
showed that no penetration peg or appressoria could still penetrate into the cuticle
(Fig. 11.9III with Bb-71). Meanwhile, other appressoria-like structures were found
(Fig. 11.8VI with Bb-100, Fig. 11.9IV with Bb-38); at the top of the germ tube, a
penetration peg-like structure was produced (Wang 1999).
Under TEM, photographs of the internal structures of appressoria or appressoria-
like structure were taken (Fig. 11.10I–III, appressoria-like structure, IV–IX appres-
soria). TEM photos presented that the pre-GT stood out, to which materials were
moved from conidium (Fig. 11.10IV). There was a short stage of vigorous nuclear
activity where the daughter nucleus spread inside a second time frame
(Fig. 11.10V). The formation of septum was followed by the migration of the
nuclei into the appressoria (Fig. 11.10IX). Inside the appressoria-like structure
(Fig. 11.10I–III), conidial materials were transmitted from the conidia to the top
part. The most frequent and biggest vacuoles could be found within the conidium
and along the germ tube. Inside, nuclear division had been finished, and daughter
nuclei could be seen in the germ tube. In late stage about 48 h, inside nuclei division
had been finished; one daughter nuclei still remained in the conidium, while another
moved through the germ tube and entered the forming appressorium. A septum
usually was formed across the end of the germ tube, beginning to separate germ
tube (appressoria) from conidia (Fig. 11.10IX). Beforehand, karyon division was
⁄
Fig. 11.8 (continued) (arrow), scale bar ¼ 1 μm; (IV) superficial view of the abdomen of
P. xylostella with Bb-71 after over 36 h under SEM, hyphae penetrating into the epicuticle
(arrow), scale bar ¼ 1 μm; (V ) superficial view of the abdomen of P. xylostella infected with
Bb-100 under SEM, conidia (arrow), scale bar ¼ 1 μm; (VI) appressoria-like structure in Bb-100
ready to invade the epicuticle of P. xylostella after over 36 h under SEM, invading appressoria-like
structure (arrow), scale bar ¼ 1 μm; (VII) appressoria (Bb-38) penetrating the epicuticle of
P. xylostella after over 36 h under SEM, a terminal swollen invading appressorium (arrow),
scale bar ¼ 1 μm; (VIII) cross section of conidia under TEM, bud of the germ tube (arrow), scale
bar ¼ 1 μm; (IX) germinated conidia under TEM, scale bar ¼ 1,500 nm; (X) thin section of
appressorium under TEM, scale bar ¼ 1 μm; conidium (C), germ tube (GT), nodule structure
(NS), hairs (HS), appressorium (AP), penetration peg (PP), contaminated conidia (CC), vacuole
(V), nuclei (N), mucilage (M), appressoria-like structure (ALS), hyphae (H) (cf. Wang 1999; You
et al. 2004)
246 L. Wang et al.
Fig. 11.9 Appressorium on the cuticle of P. xylostella contaminated by B. bassiana under SEM,
conidia (C), germ tube (GT), hairs (HS), appressorium (AP), penetration peg (PP), fusiform
appressorium (FA), mucilage (M); (I) and (II) contaminated by Bb-100; (III) contaminated by
Bb-71, melanized tip of appressorium (arrow) during invasion; (IV) contaminated by Bb-38; scale
bar = 1 μm (cf. Wang 1999; You et al. 2004)
processed prior to septum formation, but it is difficult to find. Clearly, the formation
of septum symbolizes the finished and mature stage of appressoria. Sometimes
mucus appeared at the top of appressoria (Fig. 11.10X–XII), but not in all. In
Fig. 11.9III, the top of appressoria externally turned dark in color before penetra-
tion. Appressorial wall thickness was the same as conidia, but in some appressoria,
a part of the top wall of appressorium was thickening (Fig. 11.10IX). Compared
with appressoria, the germ tube wall was thinner than the conidia (Fig. 11.10X–
XII). Inside, a great deal of vesicles could be found in maternal conidia, but in
appressoria, vesicles were rare, especially at the top (Fig. 11.10IX, X) (Wang
1999).
11.3.1.4 Penetration
Penetration is another dominant step in the course of infection in entomopathogenic

fungi. These experiments focused on the behavior of conidia of B. bassiana on the
cuticle of the second instar of P. xylostella. The penetration was observed over 36 h
contamination of host and could happen almost at any region with hole at the point
of penetration of the cuticle, including the thorax (Fig. 11.8I), head (Fig. 11.8II),
and abdomen (Fig. 11.8III–V). There were more penetrations at the rare hair region
than regions with heavy hair. There was no errant growth of hyphae without
Fig. 11.10 Germ tube and appressoria under TEM (I ) detailed germ tube, scale bar ¼ 500 nm; (II)
thin section of appressoria-like structure, scale bar ¼ 500 nm; (III) appressoria-like structure,
birdhead-like tip (arrow), scale bar ¼ 500 nm; (IV) initial appressorial structure (arrow), scale
bar ¼ 200 nm; (V ) early appressorial structure (arrow), scale bar ¼ 1 μm; (VI–VIII) appressoria-
248 L. Wang et al.
penetration observed. Penetration peg was formed at the tip or near the tip position
of appressoria (Fig. 11.8III), which was succeeding in the cuticle. In both Bb-100
and Bb-62, most germ tubes were short and thin at the top part on P. xylostella when
penetrating. It was also noted that terminal swelling on the end of the germ tube
turned dark when breaking down the cuticle of P. xylostella (Fig. 11.9III with strain
Bb-71). The invading hypha was quite simple, without any change at the top. No
such phenomenon was found in three others (strain Ba-100, Ba-38, and Ba-62). A
cross section of the cuticle of both P. xylostella (Fig. 11.10) was made to record the
differences between them at the ultrastructural level. There is a lamellate structure
under the epicuticle of the abdomen, seeing no nuance between them. Nevertheless,
the gold-coloidal technique (gold-chitinase complexes) revealed some differences.
Particles could be found in main parts of the cuticle in both pests. In early stages of
penetration, mucilage was found at the edge of the top of the germ tube contacting
with the cuticle of P. xylostella before breaching it (Fig. 11.7a); epicuticle defor-
mation was also observed. A detailed micrography revealed that mucilage was
double layer (Fig. 11.11I). Meanwhile, mucilage also appeared at no contacting part
(at the edge of the top of the germ tube) (Fig. 11.11I–III). Under the conidium, there
was a great reduction in thickness of the epicuticle of the host or even a complete
absence of cuticle material (Fig. 11.11III). The deformation of epicuticle appeared
under the conidium otherwise (Fig. 11.12I). These results suggest that (1) within the
top part of germ tube (or appressoria) would be the most active site of enzyme
metabolism and (2) results confirm the hypothesis that pathogen invasion is a
combination of enzyme and mechanical pressure (Zhai and Huang 1995). On
P. xylostella, birdhead-like appressoria were observed, but were rarely seen
(Fig. 11.9I, II). Few appressoria were presented on P. xylostella, but their shape
was the same as those generated by Bb-100. When breaching the cuticle of
P. xylostella, mucus still covered at the top of the germ tube externally
(Fig. 11.12I). After breaking down, materials inside the conidium migrated into
the germ tube, which made the germ tube fully expand and the conidium was partly
emptied. At the same time, daughter nuclei also moved into the germ tube
(Fig. 11.12II) on P. xylostella with Bb-100; mucus sealed around cleavage could
be found (Fig. 11.12III, IV). Without touching with any substance, mucilage could
be secreted by appressoria (Fig. 11.10IX); meanwhile, septum was not found. It is
proposed that mucilage material secreted by B. bassiana would assist fungi’s
infection. Near the germ tube, hyphal bodies were formed, which were full inside;
nuclei were clearly seen without any vesicles (Fig. 11.12III). In later stages (after
over 5–7 days on P. xylostella), hyphal bodies and blastospores fully filled the host
body (Fig. 11.13I under TEM) and cuticle was destroyed (Fig. 11.13II under TEM).
Fig. 11.10 (continued) like structure, scale bar ¼ 200 nm; (IX) appressoria in middle stage, scale
bar ¼ 1 μm; (X–XII) detailed appressorial structure, scale bar ¼ 1 μm; conidia (C), conidia wall
(CW), germ tube (GT), nodule structure (NS), hairs (HS), appressorium (AP), penetration peg
(PP), contaminated conidia (CC), vacuole (V), nuclei (N), fusiform appressoria (FA), mucilage
(M), septum (S) (cf. Wang 1999; You et al. 2004)
Fig. 11.11 Mucilage and appressorial structure under TEM (I ) double mucilage cover on the tip
of the germ tube (arrow), scale bar ¼ 100 nm; (II) cross section of appressorium on epicuticle of
P. xylostella infected by strain Bb-100, disappeared epicuticle part (dark arrow), scale
bar ¼ 500 nm; (III) appressorium on epicuticle of P. xylostella infected by strain Bb-62,
disappeared epicuticle part (arrow), scale bar ¼ 500 nm; conidia (C), germ tube (GT), appresso-
rium (AP), vacuole (V), mucilage (M), epicuticle (E) (cf. Wang 1999; You et al. 2004)
A study of mummified larvae demonstrated that hyphae exit first at the abdomen
region, but eventually appeared over the entire body (Wang 1999).
11.3.2 Enzyme Activities
11.3.2.1 Superficial Chitinase Profiles Under SEM
Bb-100 applied on the head of P. xylostella demonstrated an enzyme activity

(arrow) (Figs. 11.8II and 11.9II on P. xylostella), as breaking down cuticle. Find-
ings under SEM proposed that (1) releasing of chitinase on host depends on
substrate contacted, being in agreement with reports (Coudron et al. 1984;
St. Leger et al. 1996) and strain, and (2) some kinds of chitinases must be activated
by proteinase before expressing activity because of their existing form of zymogen
(Gooday et al. 1986). As consideration, there are some kind of substances that can
induce the release of chitinase on elytra, and (3) chitinase is of potential in
facilitating invasion, regardless of strain’s pathogenicity.
11.3.2.2 Chitinase and Chitin Labeling Under TEM
According to the report, chitinases from different pathogens shared 66 % identity

among them (St. Leger et al. 1996). For this reason, an antibody-ConA complex
was applied to detect chitinase activity during invasion. Unfortunately, poor results
250 L. Wang et al.
Fig. 11.12 Invasion of the cuticle of P. xylostella infected by Bb-100 under TEM (I ) initial stage
of invasion by the germ tube on the epicuticle after over 36 h, deformed epicuticle part due to
mechanical pressure by the conidium (arrow), invading hyphae (white arrow), scale bar ¼ 500 nm
(II–IV) late stages of invasion of the cuticle after over 48 h, leakage of the cuticle (arrow), (II)
scale bar ¼ 200 nm, (III) scale bar ¼ 200 nm, (IV) scale bar ¼ 100 nm; conidia (C), appressorium
(AP), vacuole (V), mucilage (M), epicuticle (E), nuclei (N), hyphal body (HB), penetrating hyphae
(PH), lamellate structure (LS) (cf. Wang 1999; You et al. 2004)
were obtained. Another method was tried instead of primer with good results.
Results under TEM indicated that chitinase activity could be detected through
infection stage on the second instar cuticle of both P. xylostella, but was rare to
see in later stages after penetration. Figure 11.14I–III provides a superficial show of
chitinase activity before breaching P. xylostella. Before germination (24 h), gold
particles are almost only distributed near conidia, and only a few particles were
observed in conidia (Fig. 11.14III). This probably gives evidence concerning the
level of chitinase activity. When germination occurred (over 36 h), large amounts
of gold particles were found within the outer layer and near the germ tube.
However, a few gold particles were found on the germ tube surface (Fig. 11.14II).
In later penetration stage, chitinase was not labeled because hyphae had penetrated
into the cuticle fully at this stage, and very little chitinase was required. Therefore,
Fig. 11.13 (I ) Rapid growth of hyphae inside the host after over 6 days under TEM, hemocyte
(arrow), scale bar ¼ 2 μm, (II) detailed view of the destroyed cuticle of P. xylostella after over
6 days under TEM, destroyed cuticle (arrow), scale bar ¼ 1 μm; hyphae body (HB), hyphae (H),
blastospore (B), epicuticle (E) (cf. Wang 1999; You et al. 2004)
Fig. 11.14 Germinated conidia under the epicuticle of P. xylostella under TEM (I ) infected by
strain Bb-100, 10 nm gold particles mainly distributed around conidia, a lot of particles on the
surface of appressoria (II) infected by strain Bb-62, 10 nm gold particles mainly distributed around
conidia, a lot of particles on the wall of conidia (III) detailed view of the cross section of the
labeled cuticle of P. xylostella, 30 nm gold particles distributed at random on the cuticle; conidia
(C), appressoria (AP), gold particle (GP), epicuticle (E), lamellate structure (LS); scale
bar ¼ 200 nm (cf. Wang 1999; You et al. 2004)
it is hard to detect the lowest enzyme activity within conidia and germ tubes
(Fig. 11.12II with strain Bb-100 on P. xylostella).
Chitin is an unbranched polysaccharide, composed primarily of β-1,4-linked N-
acetylglucosamine (NAG) residue, with an occasional glucosamine residue
(Brimacomge and Webber 1964). The best documented pathway for the degrada-
tion of chitin involves the sequential action of two separate hydrolases:
(1) endochitinase [poly-β-1,4-(2 acetamido-2-deoxy)-D-glucoside glycanhydrolase
(EC-3.2.1.14)], which produces low-molecular-weight soluble multimers of NAG,
252 L. Wang et al.
the titer from N,N-diacetyl chitobiose being predominant, and (2) chitobiase or
exochitinase [chitobiase acetylaminodeoxyglucohydrolase (EC 3.2.1.29)], which
hydrolyses the intermediates to NAG (Coudron et al. 1984). Conidia, both Bb-100
and Bb-62 germinated to form germ tubes on the surface of the third instar of
P. xylostella and penetrated cuticles within 40 h. The thin section of the cuticle at
16, 24, 36, 48, and 60 h post-inoculation was labeled with antibody to chitinase and
gold-polysaccharide complexes (dehydroxy chitin), respectively. In spite of their
66 % identity in sequence, there were still sequence differences between chitinase
in B. bassiana and Serratia marcescens, whereas in experiments, just a few gold
particle were found around conidia (Wang 1999). The failure to detect chitinase via
antibody probably is a good explanation to this menace. Firstly, gold-
polysaccharide complexes were used to show distribution of chitinase; no such
thing has been reported before. The polysaccharide applied contains a variety of
collection including titer of NAG, multimer of NAG, and glycol chitin, which can
be taken as substrate by whatever endochitinase to degrade into NAG. Therefore, it
is possible to label endochitinase in fungi by using such complexes. In addition,
gold-chitinase was employed to examine chitin distribution both in fungi (Bb100,
Bb62) and cuticle, but only later was labeled on P. xylostella (Fig. 11.14III). The
failure to label chitin in hypha hints that it is just an evaluated protecting strategy
for fear that fungi would be destroyed by chitinase released by themselves.
11.4 Metabolites
Numerous factors (slow action, potentially negative interactions with commonly

used fungicides, limited shelf life, and dependence on favorable environmental
conditions) continue to impede the commercial development and/or application of
this fungus (Wang et al. 2007). B. bassiana requires high humidity for germination,
for establishment of infection, and for sporulation and consequent epizootics, which
commonly facilitates epizootics of plant disease. These factors became bottlenecks
in the application of B. bassiana in DBM control. B. bassiana produces secondary
metabolites with insecticidal properties during the colonization of the host tissue,
which may play an important role in host mortality. Recently, the toxic substances
extracted from B. bassiana have been found to be potent against DBM in the
laboratory and at field levels. Gao et al. (2012) found that the fermentation filtrate
of strain Bb-2 had bioactive and virulent properties against the larvae of
P. xylostella with the corrected mortality of 26.7 % and 35.6 % 24 h and 48 h
after treatment with fermentation filtrate.
11.5 Conclusion and Future Prospects
Future studies should be directed toward the definition of recognition factors which
are involved in (1) the attachment; (2) chemical recognition between the emerging
hyphal tip of germination conidia and surface of epicuticle, resulting in the hyphal
tip taxis toward cuticle and penetration; (3) enzyme activity; and (4) appressoria
formation. In conclusion, the application of toxic secondary metabolites from
B. bassiana has broken through the bottlenecks in the application of B. bassiana
for the control of diamondback moth.
References
Bidochka MJ, Miranpuri GS, Khachatourians GG (1993) Pathogenicity of Beauveria bassiana

(Balsamo) Vuillemin toward lygus bug (Hem., Miridae). J Appl Entomol 115:313–317
Brimacomge JS, Webber JM (1964) Mucopolysaccharides: chemical structure, distribution and
isolation. Elsevier, Amsterdam, pp 18–42
Butt TM, Jackson C, Magan C (2001) Fungi as biocontrol agents: progress, problems and
potential. CABI Publishing, UK
Coudron TA, Kroha MJ, Ignoffo CM (1984) Levels of chitinolytic activity during development of
tree entomopathogenic fungi. Comp Biochem Physiol 79B:339–348
Fan M, Li Z (1994) Impact of nutrients and culture conditions on appressorium formation of
entomogenous fungi. J Aanhui Agric Univ 21:123–130
Gao P, Hu Q, Wang Y (2012) Virulence of spores and fermentation filtrates of Beauveria bassiana
Bb-2. Hubei Agric Sci 51:3237–3239
Gooday GW, Humphreyas M, Mcintoshw H (1986) Roles of chitinases in fungal growth.
In: Muzzarelli CJ, Gooday GW (eds) Chitin in nature and technology. Plenum, New York,
pp 83–91
Lacey LA, Goettel M (1995) Current development in microbial control of insect pests and
prospects for the early 21st century. Entomophaga 40:3–27
Large JP, Monsigny M, Prevost MC (1988) Visualization of exocellular lectins in the
entomopathogenic fungus Conidiobolus obscurus. J Histochem Cytochem 36:1419–1424
Masuda T (1998) Microbial control of diamond back moth, Plutella xylostella, by
entomopathogenic fungus, Beauveria bassiana. In: Meeting Program and Abstracts VII
Inter. Coll. Invertebr. Path. & Microb. Contr. IVth Inter. Confer. on Bacillus thuringiensis,
Sapporo, 23–28 August 1998
St. Leger RJ, Butt TM, Mark S et al (1989) Production in vitro of appressoria by the
entomopathogenic fungus Metarhizium anisopliae. Exp Mycol 13:274–288
St. Leger RJ, Roberts DW, Staples RC (1991) A model to explain differentiation of appressoria by
germlings of Metarhizium anisopliae. J Invertebr Pathol 57:299–310
St. Leger RJ, Bidochka MJ, Donald WR (1994) Germination triggers of Metarhizium anisopliae
conidia are related to host species. Microbial 140:1651–1660
St. Leger RJ, Joshi L, Gidochka MJ, Nancy W et al (1996) Characterization and ultrastructure
localization of chitinases from Metarhizium anisopliae, M. flavoviride, and Beauveria bassiana
during fungal invasion of host (Manduca sexta) cuticle. Appl Environ Microb 62(3):907–912
Wang H (1999) Ultrastructural studies on infection behaviors of Beauveria bassiana to Plutella
xylostella. Ph.D. Thesis, Fujian Agricultural University, Fuzhou
254 L. Wang et al.
Wang L, Huang J, You M, Guan X, Liu B (2007) Toxicity and feeding deterrence of crude toxin
extracts of Lecanicillium (Verticillium) lecanii (Hyphomycetes) against sweet potato whitefly
(Bemisia tabaci; Hom., Aleyrodidae). Pest Manag Sci 63:381–387
Wang L, You M, Huang J, Zhou R (2010) Diversity of entomopathogenic fungi and their
application on biological control. Acta Agric Univ Jiangxiensis 32:920–927
You M, Hou Y, Yang G (2004) Control of the population system of Diamond back moth Plutella
xylostella. Fujian Science and Technological Press, Fuzhou
Zhai J, Huang X (1995) Review on pathogenicity mechanism of entomopathogenic fungi,
Beauveria bassiana. Mycology 22:45–48
Zhai J, Huang X (1997) Studies on behavior of germination of conidia from Beauveria bassiana on
the cuticle of Heliothis zea. Acta Microbiol Sin 37:154–158
Chapter 12
Entomopathogens for Cotton Defoliators
Management
K. Sahayaraj
12.1 Introduction
Cotton plants, Gossypium hirsutum L. (Malvaceae), are one of the most important
crops cultivated in more than 80 countries (Bottrell and Adkisson 1977). China,
India, the USA, Africa, Pakistan, Brazil, Uzbekistan, Australia, Turkey, Turkmen-
istan, Greece, Argentina, Burkina and Mexico are arguably the most important
cotton-producing countries in the world (Table 12.1). It is a major world agricul-
tural crop cultivated for the harvest of lint fibres utilised extensively in the manu-
facture of apparel, household and industrial goods. Although linked biologically to
the production of cotton fibres, cottonseeds are more than a mere by-product of the
cotton harvest. Seeds ginned from the lint fibres are processed commercially for use
in animal feed, food for human consumption, and concoctions are used in the
preparation of these foods as well as numerous other home and industrial products.
During the years immediately following World War II, cotton pest control in the
world was dominated by the organochlorine insecticides. The persistence and high
toxicity of these chemicals made them effective killers of the cotton defoliators. As
a result, normally only one or two treatments per season were needed to control pest
satisfactory at a relatively low cost.
12.2 Bio-Limiting Factor of Cotton Production
One of the limitations of cotton production in various parts of the world is the insect
pest infestation. Cotton is more attractive to pests and pathogens than practically
any other plant. Worldwide entomologists have reported in excess of 1,300 species
K. Sahayaraj (*)
Crop Protection Research Centre, St. Xavier’s College, Palayamkottai 627002, India

DOI 10.1007/978-3-319-14499-3_12
256 K. Sahayaraj
Table 12.1 Cotton production (million, 480 lb. bales): selected country report
2009/ 2010/ 2011/ 2012/ 2013/ 2014/
Country name 2010 2011 2012 2013 2014 2015
China 32.0 32.5 34.0 35.0 32.0 29.5
India 24.5 27.2 29.0 28.5 29.5 28.5
United States 12.2 18.1 15.6 17.3 12.9 14.5
Pakistan 9.2 8.6 10.6 9.3 9.5 9.5
Brazil 5.5 9.0 8.7 6.0 7.5 8.3
Uzbekistan 3.9 4.1 4.2 4.5 4.2 4.2
Australia 1.8 4.2 5.5 4.6 4.1 3.1
Turkey 1.8 2.1 3.4 2.7 2.3 2.9
Turkmenistan 1.5 1.8 1.4 1.6 1.5 1.5
Greece 0.9 0.9 1.3 1.2 1.4 1.4
Argentina 1.0 1.4 1.0 0.8 1.2 1.2
Burkina 0.7 0.7 0.8 1.2 1.2 1.1
Mexico 0.5 0.7 1.2 1.0 0.9 1.1
Rest of the world 7.4 7.7 9.9 9.3 9.0 8.7
African franc 2.1 2.1 3.0 3.9 4.1 3.9
zone
EU-27 1.1 1.2 1.6 1.5 1.6 1.7
World 102.8 117.0 126.6 123.0 117.1 115.5
Source: http://www.cottoninc.com/
of insects and mites inhabiting cotton, only a handful causing economic loss. In
India and Pakistan, more than 130 and 145 species, respectively, of arthropod pests
are known to attack cotton; the most prevalent species have been listed in
Table 12.2. Any microbe or animal whose activity leads to defoliation is called as
defoliator. Defoliators population is often suppressed by a complex of parasitoids,
predators and pathogens under natural condition. However, their outbreaks can be
triggered by application of insecticides that deplete or remove the natural enemy
complex. Severe defoliator outbreaks in cotton have occurred in various parts of the
world, but the factors involved in those outbreaks are poorly understood. The high
volume usage of pesticides is often a negative aspect of cotton cultivation
worldwide.
12.3 Pesticides in Cotton Production
In cotton-producing developing countries, cotton pesticides constitute the major

part of use of agricultural chemicals (Turkey, 36 %; India, 45 %; and Egypt, 50 % of
all pesticides used in agriculture) (https://www.icac.org). Some important insecti-
cides utilised for defoliator management are aldicarb, acephate, alpha-
cypermethrin, beta-cyfluthrin, chlorpyriphos, demeton, dimethoate, endosulfan,
fenvalerate, phosphamidon, phosalone, malathion, methamidophos, thiram and
12 Entomopathogens for Cotton Defoliators Management 257
Table 12.2 List of cotton defoliators reported from different parts of the world and their
symptoms
Pest name Order/family Symptoms Country
Helicoverpa zea (Boddie) Lepidoptera: Feeding on foliage or leaves USA
(foliage feeder) Noctuidae
Spodoptera litura Boisd Lepidoptera: Foliage feeder, young larvae India, Mexico
Noctuidae in groups skeletonise leaves
and older larvae voraciously
defoliate leaves
Spodoptera exigua Lepidoptera: Leaf feeder USA
(Hübner) Noctuidae
Spodoptera littoralis Lepidoptera: Triangles cut present only at Africa
Noctuidae the front or rear of the body
Anomis flava Fab. Lepidoptera: Foliage feeder called cotton India, Brazil,
(Semilooper or surveyor Noctuidae semilooper causes signifi- Africa
caterpillar) cant loss of leaf area to
young plants; larvae with
looping action are seen on
plant parts causing circular
perforations measuring 1–
3 cm in diameter in the
leaves
Alabama argillacea Lepidoptera: Leaf feeder USA
(Hübner) (cotton leafworm) Pyraustidae
Sylepta derogata Fab. (leaf Lepidoptera: Foliage roller and feeder, India, Africa
roller or phyllophagous Pyraustidae marginal portion of leaves
caterpillars) eaten away, leaves are folded
and larvae are seen in groups
amidst faecal materials,
commonly seen on leaves at
the bottom of crop canopy at
low infestation levels, severe
infestation defoliates the
whole plant
Acontia graellsi (Feist.) Lepidoptera/ Foliage feeding India, China,
Noctuidae Africa
Agrotis ipsilon (Hufnagel) Lepidoptera/ Cut and feed leaves Cosmopolitan
(black cutworm) Noctuidae
Melanoplus spp. Orthoptera/ Cut and feed leaves India
Acridoidea
Cyrtacanthacris tatarica Orthoptera/ Defoliation of leaves, partial India
Acrididae or full
Cyrtocanthacris ranacea Orthoptera/ Feeds on leaves
Acrididae
Euproctis fraterna Lepidoptera/ Initially larvae caused India
Noctuidae skeletonisation, later
defoliate
Tarache nitidula F., Trombidiformes Feed on the underside of India,
Tetranychus urticae, leaves with a necrosed South Africa
T. neocaledonicus, and appearance
T. falcaratus. (red spider
mites)
(continued)
258 K. Sahayaraj
Table 12.2 (continued)

Pest name Order/family Symptoms Country
Polyphagotarsonemus latus Trombidiformes The leaves look chapped and Africa
torn as if cut by a knife
Myllocerus subfasciatus Coleoptera/ Marginal notching, off of India
(grey weevil) Curculionoidea, leaves
Curculionidae
Gonioctena olivacea (leaf Coleoptera Leaf feeder Pakistan
beetle)
Nisotra dilecta, Nisotra Coleoptera Make lots of holes in the Africa
uniformis, Podagrica leaves of young glandless
decolorata (flea beetles) cotton plants
Frankliniella occidentalis Thrips Feed on the abaxial surface Australia
(Pergande) of cotton cotyledons
quinalphos (Clay 2004; Ferrigno et al. 2005). Almost all of these are considered
toxic enough to be classified as hazardous by the World Health Organisation
(WHO).
Integrated pest management (IPM) strategies have been proposed for cotton pest
management (Kogan 1988, 1998; Frisbie et al. 1989) particularly in Mexico
(Williams et al. 2013) where biological control agents play an important role.
Biological control is defined as the use of living organisms or their products to
combat other organisms that are considered harmful. The living organisms typically
used are predators, parasites, parasitoids and entomopathogens. In this chapter, we
mainly focus on entomopathogens (EPs) (Butt et al. 2001) for defoliator
management.
12.4 Defoliation and Its Consequences
Herbivorous arthropods, mainly insect larvae, occasionally mites and grasshoppers,

represent a major challenge for plants in their natural environment. Almost all
herbivores, in particular chewing insects, cause substantial injury to the site of their
attack. Such mechanical wounding of plant tissues is an inevitable consequence of
herbivory, although the intensity and extent of damage is different and may vary
with the mode of feeding, e.g. sucking or chewing. It causes physical stress
(e.g. reduction in growth) and metabolic stress (e.g. decrease in alkaloid content)
which leads to loss of the leaves, indirectly reducing the yield of the crop. It varied
from variety to variety despite the fact that larvae preferred feeding on specific
leaves. Often, loss of either above- or below-ground tissue alters the commitment of
the plant to the other. Loss of leaf material caused by herbivores above ground
results in reduced root mass, while root grazing by a variety of nematodes and
insect larvae leads to lower leaf mass above ground (Geiger and Servaites 1991;
Mooney et al. 1991). Cotton has the capacity to sustain life during drought as well
as in heavy flood. This has an impact on insect feeding. Another important feature is
the production of compounds such as gossypol and tannins which are highly toxic to
pests (Ted Wilson and Carter 1991).
12.4.1 Examples of Cotton Defoliators
The beet armyworm, Spodoptera exigua (Hübner), has been an occasional pest of
cotton in the USA since the early 1900s, whereas Spodoptera litura (Boisd) is a
major pest in many Asian countries causing damage primarily as a defoliator.
Spodoptera spp. are considered predominately as leaf feeders. A tremendous
amount of research has taken place into the biology and control of this pest. The
beet armyworm, S. exigua, is a widely distributed polyphagous pest of numerous
cultivated crops, including cotton. In the past decade, several novel insecticides
have shown good activity against the beet armyworm. A partial list of these
includes chlorfenapyr, tebufenozide, emamectin benzoate, indoxacarb, spinosad,
etc. Another important defoliator from this genus is Egyptian cotton leafworm,
Spodoptera littoralis (Boisd.) (El-Guindy et al. 1982).
Grasshoppers (Orthoptera: Acrididae and Romaleidae) inflict serious damage to
plants throughout their development. They represent a major group of insect pests
on cotton (Ribeiro et al. 2013). In India, Chrotogonus spp. are considered as
important defoliators of cotton, whereas in the USA, Patrick (2004) reported
different species in cotton field. Mites are of minor importance on cotton, and
they seldom cause heavy damage. Red spider mite, Tetranychus cinnabarinus
(Tetranychidae: Acarina); wolly mite, Aceria gossypii; and yellow mite/broad
mite, Polyphagotarsonemus latus (Tarsonemidae: Acarina) are few important
mites on cotton.
12.5 Entomopathogens: An Overview
The control of insect pests with EPs is unique. Naturally occurring EPs are
important regulatory factors of insect populations. Many species are employed as
biocontrol agents of insect pests of many economically important crops worldwide.
EP is an organism [generally a bacterium, virus, protozoan, fungus, microsporidium
and nematode (Vega and Kaya 2012)] causing disease in insects:
1. Entomopathogenic fungi belong to the order Hypocreales of the Ascomycota
(Beauveria, Metarhizium, Nomuraea, Paecilomyces (¼ Isaria), Hirsutella,
Cordyceps); others include Entomophthora, Zoophthora, Pandora and
260 K. Sahayaraj
Entomophaga belonging to the order Entomophthorales of the Zygomycota.

Entomopathogenic fungi are a poorly exploited source of insecticidal proteins,
which may be used for the development of new natural insecticides and as
alternative molecules for transgenic deployment. In contrast to bacteria and
viruses, fungal biocontrol agents have a unique mode of infection. They do not
need to be ingested and can invade their host directly through the cuticle.
2. Entomopathogenic bacteria include Bacillus thuringiensis, Clostridium
bifermentans, Brevibacillus laterosporus, Chromobacterium subtsugae,
Yersinia entomophaga, species of Pseudomonas, bacteria associated with nem-
atodes of the genus Steinernema, and Heterorhabditis, i.e., Xenorhabdus and
Photorhabdus (Ruiu et al. 2013).
3. The entomopathogenic viruses include baculoviruses (nuclear polyhedrosis
virus (NPV) and the granulovirus (GV), a large group of double-stranded
DNA viruses), the majority of which have been isolated from a few insect
orders: Lepidoptera, Diptera, Hymenoptera and Coleoptera.
4. Nematodes, which are capable of killing, sterilising or seriously hampering the
development of insects and completing at least one stage of their life cycle in the
host, are called entomopathogenic nematodes (EPNs). Poinar (1979) listed nine
families of EPNs (Allontone-matidae, Diplogasteridae, Heterorhabditidae,
Mermithidae, Neotylenchidae, Rhabditidae, Sphaerularidae, Steinernematidae
and Tetradonematidae) which attack insects and kill them, sterilise or alter host
development. Out of these, only steinernematids (Steinernematidae: Rhabditida)
and heterorhabditids (Heterorhabditidae: Rhabditida) have been found to be
effective due to their special qualities like quick action, wide host range and
wide distribution.
5. Entomopathogenic protozoans include Mattesia grandis, Anthonomus grandis,
Braconidae (Nesema), etc.
12.6 Entomopathogens and Cotton Pest Management
12.6.1 Alabama argillacea
Cotton crop injured by the A. argillacea (Hubner, 1818) (Lepidoptera: Noctuidae)

considerably reduces the yield (Bourne 1921). The cotton leafworm, A. argillacea,
is considered to be one of the key pests in herbaceous cotton (G. hirsutum L. r.
latifolium, Hutch) cropping, with constant occurrence in all cotton-growing states
of Brazil (Nascimento et al. 2011). In 2000, da Silva identified entomopathogenic
viruses, bacteria, fungi and protozoa from cotton fields of Brazil. Infestation of
Bacillus cereus was reported from Colombo (Agudelo and Falcon 1977). Several
insecticides and 2 formulations of B. thuringiensis were evaluated against the
cotton leafworm, A. argillacea, at 0.024 and 0.032 kg a.i./ha as wettable powder,
and mineral oil formulations did not give a good control of the larvae, but the
reduction of the pupae was significantly 6 days after the treatment, indicating a
secondary effect on the larvae before pupation (Yamamoto et al. 1990).
In Brazil, application of B. thuringiensis (14–21 g a.i./ha) was as good as the
standard methyl parathion [parathion-methyl] (187 g a.i./ha) (Bleicher et al. 1990).
Later, César Filho et al. (2002) used Metarhizium anisopliae (Metsch.) and
Beauveria bassiana (Bals.) isolates for managing this pest. Results revealed that
the isolate 645 of B. bassiana caused the highest mortality at the highest concen-
tration, followed by isolates 634, 604 and IPA 198. The lowest lethal time for
B. bassiana and M. anisopliae was achieved by the isolates 483 (4.1 days) and
1,189 (2.0 days), respectively. The isolates 1,189, 1,022 and 866 of M. anisopliae
and 483, IPA198 and 604 of B. bassiana, at 108 and 109 conidia/ml are promising
for use in the integrated control of A. argillacea larvae, but M. anisopliae seems
more effective.
12.6.2 Anomis flava
Cotton looper A. flava is recorded from India, Australia, Spain, South Asian
countries, Africa (Algeria, Cameroon, DR Congo, Eritrea, Ethiopia, Gabon, Gam-
bia, Ghana, Kenya, La Reunion, Liberia, Madagascar, Morocco, Sierra Leone,
Somalia, South Africa, Tanzania, Tunisia, Uganda, Zimbabwe), the Philippines,
etc. Larvae feed mainly on leaves and occasionally on squares and boll surfaces.
They prefer older leaves, and therefore, their damage will progress upwards on the
plant. Cotton yield can be reduced if leaf tissue loss is excessive. Generally, plants
are more sensitive earlier and increasingly less sensitive later. As a rough guide,
leaf area loss of greater than 10–15 % could result in yield loss if it occurred before
crop cut-out. After cut-out, losses of up to 15–20 % could be tolerated with low risk.
According to Deutscher et al. (1999), unsprayed cotton fields in Australia have as
much as 80 % of defoliation under infestation of A. flava.
In India, Umesh Chandra and Regupathy (2007) conducted an experiment to
investigate the Cry 1Ac toxin expression and its manifestation in A. flava larval
susceptibility, by excised leaf technique. Results revealed that toxin caused cent per
cent mortality to third instar, A. flava. B. thuringiensis (Bt) products are very
effective against this pest (http://www.aciar.gov.au/files/mn-157/imp11.html).
12.6.3 Helicoverpa zea
Corn earworm H. zea (Boddie) is a well-known pest of corn and cotton particularly
in the Southern United States (Swenson et al. 2013). It attacks both non-Bt and Bt
cotton. However, occurrence of larvae and ear damage on Bt corn was significantly
lower than on non-Bt plants, and there were no significant differences between pure
stands of Bt and ‘RIB’ plantings across all trials (Yang et al. 2014). B. thuringiensis
262 K. Sahayaraj
(Bt) toxin has reduced the use of synthetic insecticide on transgenic crops to target
Helicoverpa spp., the major insect pest of cotton in Australia (Mensah and Austin
2012). This pest has been utilised for the mass production of entomopathogenic
viruses (Reid et al. 2013), because it has susceptibility against Bt (Bailey
et al. 1998).
Fungal metabolite sclerotia produced by Aspergillus spp. have proven to be a
rich source of novel anti-insect compounds with activity against H. zea. Similarly,
the hexane and chloroform extracts of Eupenicillium crustaceum displayed signif-
icant anti-insect activity in assays against this pest. Further, in dietary assay,
reduction in weight gain and reduction in feeding rate were also observed in
H. zea (Wang et al. 1995). Champlin and Grula (1979) reported that beauvericin
was not toxic to H. zea and that bassianolide caused temporary atony.
For the first time, Pekrul and Grula (1979) reported the mechanism of action of
fungi on this pest. Entomopathogenic fungus Beauveria bassiana (Balsamo)
Vuillemin depicted direct penetration through the integument of a corn earworm,
H. zea, without appressorial formation.
Steinernema riobravis was isolated from soil samples in corn fields near Wes-
laco, Texas (Cabanillas et al. 1994). Since then, successful results have been
obtained with S. riobravis for the control of corn earworm (Cabanillas and Raulston
1994). Cabanillas and Raulston (1994) observed that prepupae of H. zea (Boddie)
exhibited susceptibility against EPN. Later, two EPNs, S. riobravis and
S. carpocapsae, were compared for their ability to parasitise corn earworm,
H. zea (Boddie) prepupae and pupae, in corn plots at the Lower Rio Grande Valley
of Texas (Cabanillas and Raulston 1996). Parasitism was higher when S. riobravis
was applied at 200,000 IJ/m2 through furrow irrigation (97 %) or post-irrigation
(95 %) than when nematodes were sprayed onto the soil before irrigation (82 %).
Parasitism of corn earworm prepupae by S. riobravis persisted up to 36 days after
application and was higher in the treated plots (80 %) than the natural parasitism of
the control plots (14 %). These results show that at high field soil temperatures,
S. riobravis is more effective against corn earworm than S. carpocapsae.
12.6.4 Spodoptera spp.
12.6.4.1 S. littoralis (Boisd.)
Cotton leafworm or Egyptian leafworm, S. littoralis (Boisd.), a polyphagous insect

herbivore, consumes large amounts of plant material in a short time (says Anderson
and Alborn 1999; Shao et al. 2014). B. bassiana isolates have proven to be
pathogenic to fall armyworm, S. frugiperda (J. E. Smith) (Rodrigues and Pratissoli
1989; Franca et al. 1989; Faria et al. 1992). Nuclear polyhedrosis virus (NPV) of
S. littoralis (1.2 106–8 polyhydra/larva), fed to third or fifth instar larvae, caused
mortality of 54–100 % or 40–73 % of these larvae, respectively. It also reduced
pupal weight, increased adult deformities and reduced fecundity (Abul-Nasr
et al. 1979). Later in Egypt, a locally produced wettable powder formulation of an

NPV [5 1011, 1 1012 and 5 1012 polyhedral inclusion bodies (p.i.b.)/ha] was
applied to control the Egyptian cotton leafworm, S. littoralis, using a knapsack
sprayer fitted with a cotton tail boom. An application rate of 1 1012 p.i.b./ha
reduced the level of this pest (Jones et al. 1994).
The crude soluble protein of M. anisopliae, B. bassiana, B. brongniartii and
Scopulariopsis brevicaulis was screened against S. littoralis larvae. The extracts
from two M. anisopliae and two B. bassiana isolates gave significant mortalities
when either applied on leaf discs or incorporated into artificial diet (Quesada-
Moraga et al. 2006). Further, they recorded in leaf disc assays that this extract
exhibited strong dose-related toxic and antifeedant activity against the larvae. Not
only the antifeeding index was dose related, but it significantly increased over time
in a dose-related manner. The crude extract when exposed to higher temperature or
protease treatment lost toxicity, indicating that toxicity was protein mediated.
Along with the above said impact, a progressive bleeding of the midgut epithelium
into the gut lumen was observed along with the lysis of the epithelium and
deterioration of the microvilli. Recently, Asi et al. (2013) critically evaluated the
potential of entomopathogenic fungi, Isaria fumosorosea (¼ Paecilomyces
fumosoroseus (Wize) Brown and Smith), B. bassiana, M. anisopliae Sorokin and
Lecanicillium lecanii (Zimmerman) (¼ Verticillium lecanii) for the biocontrol of
various life stages of S. litura on cotton.
Chitinolytic bacterial strains induced S. littoralis larval mortality when com-
bined with B. thuringiensis (Sneh et al. 1983) or individual Bt strains (Kalfon and
De Barjac 1985; Sneh et al. 1991). This shows that we can use either bacteria or
virus or their combination to reduce this pest on cotton. Previously, Moore and
Navon (1973) observed that ten different laboratory strains of B. thuringiensis
caused moderate mortality when compared to control. Later, Keller et al. (1996)
explained how B. thuringiensis δ-endotoxins in crystalline and noncrystalline forms
kill S. littoralis. But later, the pest developed resistance against Bt (Muller-Cohn
et al. 1996). As a result, many synthetic biopesticides (e.g. methoxyfenozide,
tebufenozide, spinosad, limonoids) have been on use for management. However,
other entomopathogens have been utilised for this pest management. For instance,
laboratory strains of M. anisopliae, M. flaviviridae, P. farinosis, B. bassiana, and
B. brongniartii (Amer et al. 2008), B. subtilis strain NRC313 (BS NRC313) and
B. thuringiensis strain NRC335 (BT NRC335) (Abd El-Salam et al. 2011), new
strain CCM 8367 of I. fumosorosea (Hussein et al. 2013), Bt var. kurstaki (Btk2,
Btk3 and Btk66) and Bt var. mexicanensis (Btm27), in addition to two reference
strains (4D20 and 4AC1) (Alfazairy et al. 2013), at Egypt, were tested against this
pest and showed insecticidal activity. In Saudi Arabia, using bacterial
transconjugant technology, AlOtaibi (2013) showed combined effects of crystals
+ endospores of B. thuringiensis serovar kurstaki, B. subtilis and four of their
transconjugants against this pest which showed higher mortality, and this factor
was important to be considered in designing resistance management strategies.
Similarly, nematodes also have been in use for this management. In 2006, Razek
recorded infectivity of nematodes against this pest. In laboratory studies,
264 K. Sahayaraj
demonstrated that five native EPN species/isolates caused 100 % mortality of

S. cilium larvae, a soil surface-feeding pest of turfgrass. At 25 infective juveniles/
cm2 applied to sod, two selected Turkish species, Steinernema carpocapsae and
Heterorhabditis bacteriophora (Sarigerme isolate), showed 77 % and 29 % average
larval mortality, respectively (Gulcu et al. 2014).
12.6.4.2 S. litura (Fab.)
Endophytic fungi are a group of microbial plant symbionts that occur in living
tissues of plants without causing visible disease symptoms. S. litura (F.) fed on
leaves of Nigrospora oryzae- and Cladosporium uredinicola-infected plants
exhibited abnormalities such as change in shape, extensive vacuolisation and
necrosis in significantly higher percentage of haemocytes (Thakur et al. 2014).
Entomopathogenic fungi B. bassiana at four different concentrations (2.4 107,
2.4 106, 2.4 105, 2.4 104 conidia/ml) were tested against S. litura larvae.
Results revealed that the fungi reduced pupation (43.33 %) at 2.4 107 conidia/
ml, caused mortality, adult malformation and completely arrested fecundity
(Malarvannan et al. 2010).
B. thuringiensis (Bt) is a microbial pesticide widely used to control crop pests.
Its strains have good biocontrol activity against crop insect pest and, however, lack
some desirable characteristics that are found in B. subtilis. Revathi et al. (2013),
using protoplast fusion technique, fused B. thuringiensis with a strain of B. subtilis,
and the fusants produced almost 95 % mortality in first instar larvae. Baculovirus
infection not only disturbs moulting but also affects digestive physiology
(Subrahmanyam and Ramakrishnan 1981). Similarly in another study, a transgenic
B. bassiana strain (BbV28) expressing Vip3Aa1 (a Vip3A toxin) was created to
infect the larvae of S. litura through conidial ingestion and cuticle adhesion and
tested against this pest. Feeding reduced the LC50 of the transformant by 17.2- and
1.3-fold on days 3 and 7, respectively. Median lethal times (LT50s) of BbV28 were
shortened by 23–35 %, declining with conidial concentrations. The larvae infected
by ingestion of BbV28 conidia showed typical symptoms of Vip3A action,
i.e. shrinkage and palsy. However, neither LC50 nor LT50 trends differed between
BbV28 and its parental strain if the infection occurred through the cuticle only. Our
findings indicate that fungal conidia can be used as vectors for spreading the highly
insecticidal Vip3A protein for the control of foliage feeders such as S. litura (Qin
et al. 2010).
Though NPV alone has more impact on S. litura (Sahayaraj and Joseph 2003),
Senthil-Nathan and Kalaivani (2005, 2006) studied the combined effect of NPV and
azadirachtin and suggested this combination for this pest management. Similar
concept was also proposed by Gopalakrishnan et al. (2013). They reported that
extracts of Annona, Chrysanthemum, Datura, Jatropha, Neem, Parthenium,
Pongamia, Tridax and Vitex; and plant growth-promoting (PGP) bacteria, viz.,
B. subtilis (BCB-19), B. megaterium (SB-9), Serratia marcescens (HIB-28) and
Pseudomonas spp. (SB-21), and fungus (M. anisopliae), were evaluated for their
efficacy against S. litura and showed high mortality along with weight reduction of
the larvae (73 % and 91 %). It was therefore concluded that the aforementioned six
botanicals and five entomopathogens have great potential in the management of
S. litura. Further, it was proposed that botanicals and microorganisms have the
capability to synthesise biologically active secondary metabolites such as antibi-
otics, herbicides and pesticides.
12.6.5 Acontia graellsii
Cotton semilooper (A. graellsii ¼ Acontia draellsii, Xanthodes graellsii, Xanthodes

fimbriata, Xanthodes innocens) has been recorded from various parts of India and
Africa (Equatorial Guinea, Ethiopia, Gambia, Ghana, La Reunion, Madagascar,
Malawi, Mauritania, Nigeria, Sierra Leone, Somalia, South Africa, Sudan, Zambia,
Zimbabwe). However, not much work has been carried out in Africa for this pest
management. Initially, occurrence of the entomopathogenic fungi Nomuraea rileyi
(Farlow) Samson on A. graellsii F. (Noctuidae: Lepidoptera) (Gopalakrishnan and
Narayanan 1988) was reported.
12.6.6 Sylepta derogata
Cotton leaf roller, S. derogata (Fabricius) (Lepidoptera: Crambidae), is distributed

in India, Nigeria and China. In India, chlorpyriphos, dichlorvos, fenitrothion,
monocrotophos, quinalphos, phenthoate, phosalone, tetrachlorvinphos, dicrotophos
and endosulfan are recommended for this pest management. In China, along with
few insecticides (flufenoxuron, dichlorvos, cypermethrin and abamectin),
B. thuringiensis has been utilised for this control and showed good results
(Wu et al. 2008). In India, P. farinosus was shown to infect larvae of S. derogata
(Kuruvilla and Jacob 1980). Later, Jiji et al. (2005) identified B. bassiana as one of
the best fungi for this pest control.
12.6.7 Agrotis ipsilon
Black cutworm is distributed in Europe, China, India, Canada and North America.
It has a wide host range. Nearly all vegetables can be consumed, and this species
also feeds on alfalfa, clover, cotton, rice, sorghum, strawberry, sugar beet, tobacco,
and sometimes grains and grasses. Larvae can consume over 400 cm2 of foliage
during their development, but over 80 % occurs during the terminal instar and about
10 % in the instar immediately preceding the last. Kunkel et al. (2004) reported that
the black cutworm, A. ipsilon, is less susceptible to the EPN, S. carpocapsae, when
266 K. Sahayaraj
it consumes the endophytic grass. Two entomopathogenic fungi, M. anisopliae

and B. bassiana were tested against this insect. Results show that the damage was
higher in the earlier stages of seedling growth, whereas in later stages they gave
good protection to the crop (Viji and Bhagat 2001). Tripathi et al. (2003) utilised
B. thuringiensis (Dipel 8L, subsp. kurstaki) against A. ipsilon for 2 years in Garhwal
Himalayan region, Uttar Pradesh, India, and they succeeded to manage this pest
under field conditions.
12.6.8 Mites
Cotton in the early stage as well as at the late stage is attacked by mites. They are
generally found on the undersurface of leaves wire fine webbings. During heavy
infestation, they may be found all over the leaf surface. Mites puncture the leaf
tissue and the oozing plant sap is sucked. Removal of plant sap with chlorophyll and
other plant pigments results in characteristic blocking with reddish bronze discol-
oration of leaves. Severe infestation leads to premature defoliation of leaves.
Application of dicofol or wettable sulphur reduces the mite incidence and further
infestation.
In China, aerial conidia of isolates of B. bassiana (Bb734 and Bb2860) and
M. anisopliae (Ma456 and Ma759) was mixed with an emulsifiable oil and sprayed
in block-randomised triple plots of two irrigated cotton fields for the control of
summer populations of cotton spider mites, mainly Tetranychus truncates and
T. turkestani. Results revealed that overall means of relative efficacies during the
periods of both trials were 85.8 % (77.9–94.9 %) and 88.0 % (82.4–94.0 %) for
Ma456 and 77.9 % (68.6–89.6 %) and 85.7 % (77.8–87.7 %) for Bb734 (Shi
et al. 2008). Very recently, Bt has been utilised to manage cotton mites at field
level in Brazil (Agostini et al. 2014).
12.6.9 Grasshoppers
Grasshoppers are considered as occasional as well as seasonal pests of cotton.

Grasshoppers feed on foliage, most often on the edges of fields near pasture areas
or roadsides. They seldom cause economically significant injury. Since grasshop-
pers are highly mobile and migratory pests, no specific entomopathogens have been
recommended so far. However, in China, a new species of Sporothrix was isolated
from cotton-dwelled grasshoppers (Huang and Zhen 1997).
12.7 Conclusion and Recommendations
The demand for safe and ‘biologically’ healthy foods has stimulated an increase of
research on biological control of pests. Pest control through these microorganisms,
naturally found in soil, is an important ecosystem service that maintains the stability
of agricultural systems and has the potential to mitigate costs for control of pests.
Considering the above-mentioned available literature, the following recommenda-
tions are put forth for cotton defoliator management worldwide:
1. Since not much work has been available on A. graellsii in Africa, an important pest
of cotton, available entomopathogens can be utilised for this pest management.
2. A. argillacea is an important pest of cotton in Brazil. Hence, we recommend to
utilise as many as entomopathogens for the management.
3. Many protozoans and nematodes can be utilised in cotton defoliators control.
4. Physical, chemical and molecular mechanism involved in the action of EPs on
cotton defoliators should be studied to utilise and popularise the microbial
insecticides among farmers.
5. Specificity of EPs against the cotton pest should be known.
6. Compatibility with other pest management components can be known for cotton
defoliators.
7. Agro-climate-based EPs should be identified to manage the defoliators at dif-
ferent locations.
8. Mite infestation can be considered seriously and can be reduced using the
proposed option.
Acknowledgement The author is grateful to the management, St. Xavier’s College,
Palayamkottai, for the laboratory support and encouragement.
References
Abd El-Salam AME, Nemat AM, Magdy A (2011) Potency of Bacillus thuringiensis and Bacillus
subtilis against the cotton leafworm, Spodoptera littoralis (Bosid.) Larvae. Arch Phytopathol
Plant Protect 44:204–215
Abul-Nasr SE, Ammar ED, Abul-Ela SM (1979) Effects of nuclear polyhedrosis virus on various
developmental stages of the cotton leafworm Spodoptera littoralis (Boisd.). Z Angew Entomol
88:181–187. doi:10.1111/j.1439-0418.1979.tb02494.x
Agostini LT, Duarte RT, Volpe HXL, Agostini TT, de Carvalho GA, Abrahão YP, Polanczyk RA
(2014) Compatibility among insecticides, acaricides, and Bacillus thuringiensis used to control
Tetranychus urticae (Acari: Tetranychidae) and Heliothis virescens (Lepidoptera: Noctuidae)
in cotton fields. Afr J Agric Res 9:941–949
Agudelo F, Falcon LA (1977) Some naturally occurring insect pathogens in Colombia. Turrialba
27:423–424
Alfazairy AA, El-Ahwan AM, Mohamed EA, Zaghloul HA, El-Helow ER (2013) Microbial
control of the cotton leafworm Spodoptera littoralis (Boisd.) by Egyptian Bacillus
thuringiensis isolates. Folia Microbiol 58:155–162
268 K. Sahayaraj
AlOtaibi SA (2013) Mortality responses of Spodoptera litura following feeding on BT-sprayed

plants. J Basic Appl Sci 9:195–215
Amer MM, El-Sayed TI, Bakheit HK, Moustafa SA, El-Sayed YA (2008) Pathogenicity and
genetic variability of five entomopathogenic fungi against Spodoptera littoralis. Res J Agric
Biol Sci 4:354–367
Anderson P, Alborn H (1999) Effects on oviposition behaviour and larval development of
Spodoptera littoralis by herbivore-induced changes in cotton plants. Entomol Exp Appl
92:45–51
Asi MR, Bashir MH, Afzal M, Zia K, Akram M (2013) Potential of entomopathogenic fungi for
biocontrol of Spodoptera litura Fab. (Lepidoptera: Noctuidae). J Animal Plant Sci 23:913–918
Bailey WD, Zhao G, Carter LM, Gould F, Kennedy GG, Roe RM (1998) Feeding disruption
bioassay for species and Bacillus thuringiensis resistance diagnosis for Heliothis virescens and
Helicoverpa zea in cotton (Lepidoptera: Noctuidae). Crop Prot 17:591–598
Bleicher E, de Jesus FMM, de Sousa SL (1990) Use of selective insecticides to control the cotton
leaf worm. Pesq Agrop Brasileira 25:277–280
Bottrell DG, Adkisson PL (1977) Cotton insect pest management. Annu Rev Entomol 22:451–481
Bourne BA (1921) Report of the assistant director of agriculture on the entomological and
mycological work carried out during the season under review. Report, 1919–1920. Department
of Agriculture, Barbados, pp 10–31
Butt TM, Jackson C, Magan N (2001) Fungi as biocontrol agents: progress, problems and
potential. CAB International, Wallingford
Cabanillas HE, Raulston JR (1994) Pathogenicity of Steinernema riobravis against corn earworm,
Helicoverpa zea (Boddie). Fundam Appl Nematol 17:219–223
Cabanillas HE, Raulston JR (1996) Evaluation of Steinernema riobravis, S. carpocapsae, and
irrigation timing for the control of corn earworm, Helicoverpa zea. J Nematol 28:75
Cabanillas HE, Poinar GO Jr, Raulston JR (1994) Steinernema riotrravis n. sp. (Rhabditida:
Steinernematidae) from Texas. Fundam Appl Nematol 17:123–131
César Filho E, Marques EJ, Barros R (2002) Selection of Metarhizium anisopliae (metsch.) and
Beauveria bassiana (bals.) isolates to control Alabama argillacea (huebner) caterpillars. Sci
Agric 59:457–462
Champlin FR, Grula EA (1979) Noninvolvement of beauvericin in the entomopathogenicity of
Beauveria bassiana. Appl Environ Microbiol 37:1122–1125
Clay J (2004) World agriculture and the environment: a commodity-by-commodity guide to
impacts and practices. Island Press, Washington, DC
da Silva CAD (2000) Entomopathogenic microorganisms associated with insects and mites of
cotton. Documentos-Embrapa Algodão 77:45
Deutscher SL, Wilson S, Whiteside ST, Mansfield M, Rossiter WL (1999) Pest and beneficial
guide. Australian Cotton Cooperative Research Centre, Narrabri, p 218
El-Guindy MA, Madi SM, Keddis ME, Issan YH, Abdel-Satto MM (1982) Development of
resistance to pyrethroids in field populations of the Egyptian cotton leafworm, Spodoptera
littoralis Boisd. Int Pest Control 24:6–11
Faria LLF, de Oliveia JV, Barros R (1992) Patogenicidade do fungo Beauveria bassiana (Bals.)
Vuill., em lagartas de Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) sob
condições de laboratorio. Caderno Ômega 4:207–217
Ferrigno S, Ratter SG, Ton P, Vodouhe DS, Williamson S, Wilson J (2005) Organic cotton: a new
development path for African Smallholders? vol 120, Gatekeeper series. International Institute
for Environment and Development, London
Franca MM, Tigano MS, Carvalho RS (1989) Suscetibilidade de Spodoptera frugiperda aos
fungos entomopatogênicos Beauveria bassiana e Nomuraea rileyi. In: Congresso Brasileiro
de Entomolgia, vol 12. Belo Horizonte, Resumos. SEB, Belo Horizonte, p 254
Frisbie RE, El Zik K, Wilson LT (1989) Integrated pest management systems and cotton produc-
tion. Wiley, New York, p 437
Geiger DR, Servaites JC (1991) Carbon allocation and response to stress. In: Mooney HA, Winner
WW (eds) Response to stress. Academic, New York, pp 103–127
Gopalakrishnan M, Narayanan K (1988) Occurrence of the entomopathogenic fungi Nomuraea
rileyi (Farlow) Samson on Acontia graellsii F. (Noctuidae: Lepidoptera) and Beauveria
bassiana (Balsamo) Vuill. on Myllocerus subfaciatus G. (Curcolionidae: Coleoptera). J Biol
Control 2:58–59
Gopalakrishnan S, Rao GR, Humayun P, Rao VR, Alekhya G, Jacob S, Rupela O (2013) Efficacy
of botanical extracts and entomopathogens on control of Helicoverpa armigera and
Spodoptera litura. Afr J Biotechnol 10:16667–16673
Gulcu B, Ulug D, Hazir C, Karagoz M, Hazir S (2014) Biological control potential of native
entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) against Spodoptera
cilium (Lepidoptera: Noctuidae) in turfgrass. Biocontrol Sci Technol 24(8):965–970
Huang BL, Zhen MZL (1997) Sporothrix chondracris, a new entomopathogenic fungus on cotton.
Hycosena 16:88–90
Hussein HM, Zemek R, Habuštová SO, Prenerová E, Adel MM (2013) Laboratory evaluation of a
new strain CCM 8367 of Isaria fumosorosea (syn. Paecilomyces fumosoroseus) on Spodoptera
littoralis (Boisd.). Arch Phytopathol Plant Protect 46:1307–1319
Jiji T, Praveena R, Naseema A, Anitha N (2005) Beauveria bassiana (Bals) Vuill. on Sylepta
derogata Fab. and its cross infectivity on other major pests of vegetables. Insect Environ
11:144
Jones KA, Irving NS, Grzywacz D, Moawad GM, Hussein AH, Fargahly A (1994) Application
rate trials with a nuclear polyhedrosis virus to control Spodoptera littoralis (Boisd.) on cotton
in Egypt. Crop Prot 13:337–340
Kalfon AR, De Barjac H (1985) Screening of the insecticidal activity of Bacillus thuringiensis
strains against the egyptian cotton leaf worm Spodoptera littoralis. Entomophaga 30:177–186
Keller M, Sneh B, Strizhov N, Prudovsky E, Regev A, Koncz C, Zilberstein A (1996) Digestion of
δ-endotoxin by gut proteases may explain reduced sensitivity of advanced instar larvae of
Spodoptera littoralis to CryIC. Insect Biochem Mol Biol 26:365–373
Kogan M (1988) Ecological theory and integrated management – theory and practice. Wiley,
New York, p 362
Kogan M (1998) Integrated pest management: historical perspectives and contemporary develop-
ments. Annu Rev Entomol 43:243–270
Kunkel BA, Grewal PS, Quigley MF (2004) A mechanism of acquired resistance against an
entomopathogenic nematode by Agrotis ipsilon feeding on perennial ryegrass harboring a
fungal endophyte. Biol Control 29:100–108
Kuruvilla S, Jacob A (1980) Pathogenicity of the entomogenous fungus Paecilomyces farinosus
(Dickson ex Fries) to several insect pests. Entomon 5:175–176
Malarvannan S, Murali PD, Shanthakumar SP, Prabavathy VR, Nair S (2010) Laboratory evalu-
ation of the entomopathogenic fungi, Beauveria bassiana against the Tobacco caterpillar,
Spodoptera litura Fabricius (Noctuidae: Lepidoptera). J Biopestic 3:126–131
Mensah RK, Austin L (2012) Microbial control of cotton pests. Part I: use of the naturally
occurring entomopathogenic fungus Aspergillus sp. (BC 639) in the management of
Creontiades dilutus (Stal) (Hemiptera: Miridae) and beneficial insects on transgenic cotton
crops. Biocontrol Sci Technol 22:567–582
Mooney HA, Winner WE, Pell EJ (eds) (1991) Response of plants to multiple stresses. Academic,
New York, p 422
Moore I, Navon A (1973) Studies of the susceptibility of the cotton leafworm, Spodoptera
littoralis (Boisduval), to various strains of Bacillus thuringiensis. Phytoparasitica 1:23–32
Muller-Cohn J, Chaufaux J, Buisson C, Gilois N, Sanchis V, Lereclus D (1996) Spodoptera
littoralis (Lepidoptera: Noctuidae) resistance to CryIC and cross-resistance to other Bacillus
thuringiensis crystal toxins. J Econ Entomol 89:791–797
270 K. Sahayaraj
Nascimento AR, Ramalho FS, Azeredo TL, Fernandes FS, Júnior JN, Silva CAD, Malaquias JB
(2011) Feeding and life history of Alabama argillacea (Lepidoptera: Noctuidae) on cotton
cultivars producing colored fibers. Ann Entomol Soc Am 104:613–619
Patrick CD (2004) Grasshoppers and their control. http://www.hdl.handle.net/1969.1/87825
Pekrul S, Grula EA (1979) Mode of infection of the corn earworm (Heliothus zea) by Beauveria
bassiana as revealed by scanning electron microscopy. J Invertebr Pathol 34:238–247
Poinar GO Jr (1979) Steinernematids and heterorhabditis have been observed to infect over
200 species of insects from several orders. In: Swan JL, Kaya HK (eds) A few other arthropods
are also susceptible to EPNs: a handbook of techniques, CRC Press, Inc., p 277
Qin Y, Ying SH, Chen Y, Shen ZC, Feng MG (2010) Integration of insecticidal protein Vip3Aa1
into Beauveria bassiana enhances fungal virulence to Spodoptera litura larvae by cuticle and
per os infection. Appl Environ Microbiol 76:4611–4618
Quesada-Moraga E, Carrasco-Dı́az JA, Santiago-Álvarez C (2006) Insecticidal and antifeedant
activities of proteins secreted by entomopathogenic fungi against Spodoptera littoralis (Lep.,
Noctuidae). J Appl Entomol 130:442–452. doi:10.1111/j.1439-0418.2006.01079.x
Razek AS (2006) Infectivity prospects of both nematodes and bacterial symbionts against cotton
leafworm, Spodoptera littoralis (Biosduval) (Lepidoptera: Noctuidae). J Pest Sci 79:11–15
Reid S, Chan L, van Oers MM (2013) Production of entomopathogenic viruses. In: Morales-
Ramos JA, Rojas MG, Shapiro-Ilan DI (eds) Mass production of beneficial organisms:
invertebrates and entomopathogens. Academic, London, pp 437–482
Revathi K, Chandrasekaran R, Thanigaivel A, Arunachalam Kirubakaran S, Senthil-Nathan S
(2013) Biocontrol efficacy of protoplast fusants between Bacillus thuringiensis and Bacillus
subtilis against Spodoptera litura Fabr. Arch Phytopathol Plant Protect 47(11):1365–1375
Ribeiro RC, Lemos WDP, Poderoso JCM, Pikart TG, Zanuncio JC (2013) New record of
grasshopper (Orthoptera: Acrididae & Romaleidae) defoliators and population dynamics of
insects on crops of Heliconia spp in the Amazon. Florida Entomol 96:225–228
ogenos Beauveria
Rodrigues C, Pratissoli D (1989) Avaliação de patogenicidade dos fungos entom
bassiana e Metarhizium anisopliae sobre Spodoptera frugiperda (lagarta do cartucho). In:
Congresso Brasileiro De Entomologia, vol 12. Belo Horizonte, Resumos. SEB, Belo
Horizonte, p 223
Ruiu L, Satta A, Floris I (2013) Emerging entomopathogenic bacteria for insect pest management.
Sahayaraj K, Joseph M (2003) Impact of (S) NPV on Spodoptera litura (Fab.) mortality and flora.
Nat Conserv 15:43–50
Senthil-Nathan S, Kalaivani K (2005) Efficacy of nucleopolyhedrovirus (NPV) and azadirachtin
on Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Biol Control 34:93–98
Senthil-Nathan S, Kalaivani K (2006) Combined effects of azadirachtin and nucleopolyhedrovirus
(SpltNPV) on Spodoptera litura Fabricius (Lepidoptera: Noctuidae) larvae. Biol Control
36:94–104
Shao Y, Arias-Cordero E, Guo H, Bartram S, Boland W (2014) In vivo pyro-SIP assessing active
gut microbiota of the cotton leafworm, Spodoptera littoralis. PLoS One 9:e85948
Shi WB, Zhang LL, Feng MG (2008) Field trials of four formulations of Beauveria bassiana and
Metarhizium anisopliae for control of cotton spider mites (Acari: Tetranychidae) in the Tarim
Basin of China. Biol control 45:48–55
Sneh B, Schuster S, Gross S (1983) Improvement of the insecticidal activity of Bacillus
thuringiensis var. entomocidus on larvae of Spodoptera littoralis (Lepidoptera, Noctuidae)
by addition of chitinolytic bacteria, a phagostimulant and a UV-protectant. Z Angew Entomol
96:77–83. doi:10.1111/j.1439-0418.1983.tb03644.x
Sneh B, Schuster S, Broza M (1991) Insecticidal activity of Bacillus thuringiensis strains against
the Egyptian cotton leafworm, Spodoptera littoralis Boisd. (Lep. Noctuidae). Entomophaga
26:179–190
Subrahmanyam B, Ramakrishnan N (1981) Influence of a baculovirus infection on molting and
food consumption by Spodoptera litura. J Invertebr Pathol 38:161–168
Swenson SJ, Prischmann-Voldseth DA, Musser FR (2013) Corn earworms (Lepidoptera:

Noctuidae) as pests of soybean. J Integr Pest Manag 4:D1–D8
Ted Wilson L, Carter F (1991) Leaf feeding insects and mites. Phys Today 2:1–8
Thakur A, Singh V, Kaur A, Kaur S (2014) Suppression of cellular immune response in
Spodoptera litura (Lepidoptera: Noctuidae) larvae by endophytic fungi Nigrospora oryzae
and Cladosporium uredinicola. Ann Entomol Soc Am 107:674–679
Tripathi DM, Bisht RS, Mishra PN (2003) Bio-efficacy of some synthetic insecticides and
bio-pesticides against black cutworm, Agrotis ipsilon infesting potato (Solanum tuberosum)
in Garhwal Himalaya. Indian J Entomol 65:468–473
Umesh Chandra S, Regupathy A (2007) Exploring the Bt cotton potential for the management of
semilooper complex, Anomis flava Fab. and Tarache nitidula Fab. In: World cotton research
conference-4, 10–14 September 2007. International Cotton Advisory Committee (ICAC),
Lubbock
Vega FE, Kaya HK (2012) Insect pathology, 2nd edn. Elsevier, London
Viji CP, Bhagat RM (2001) Bioefficacy of some plant products, synthetic insecticides and
entomopathogenic fungi against black cutworm, Agrotis ipsilon larvae on maize. Indian J
Entomol 63:26–32
Wang H, Gloer JB, Wicklow DT, Dowb PF (1995) Aflvinines and other antiinesctan metabolites
from the ascostroma of Eupenicillium crustaceum and related species. Appl Environ Microbiol
61:4429–4435
Williams T, Arredondo-Bernal HC, Rodrı́guez-del-Bosque LA (2013) Biological pest control in
Mexico. Annu Rev Entomol 58:119–140
Wu J-H, Huang Z, Ren S-X, Zhou H-P (2008) Control of different insecticides in field on Sylepta
derogata population. J South China Agric Univ 02:6
Yamamoto PT, Benetoli I, Fernandes OD, Gravena S (1990) Effect of Bacillus thuringiensis and
various insecticides on the cotton leafworm (Alabama argillacea) and its predators.
Ecossistema Faculdade de Agronomia e Zootecnia Manoel Carlos Goncalves, Brazil 15:36–44
Yang F, Kerns DL, Head GP, Leonard BR, Niu Y, Huang F (2014) Occurrence, distribution, and
ear damage of Helicoverpa zea (Lepidoptera: Noctuidae) in mixed plantings of non-Bt and Bt
corn containing Genuity SmartStax™ traits. Crop Prot 55:127–132
Chapter 13
Entomopathogenic Nematodes and Their
Bacterial Symbionts as Lethal Bioagents
of Lepidopteran Pests
Sharad Mohan
13.1 Introduction
Entomopathogenic nematodes (EPN) are soil-dwelling parasites of a wide range of

insects. Their ubiquity and insecticidal specificity makes them ideally suited to
form part of integrated pest management programmes. Found widely in natural and
agricultural ecosystems across the world, they are relatively specific to target pests
and non-toxic to humans and the environment. Although EPN have been described
from 23 nematode families (Koppenhöfer 2007), those belonging to the families
Steinernematidae (Chitwood and Chitwood 1937) and Heterorhabditidae (Poinar
1976) are the ones most commonly studied due to their significant biocontrol
potential. The nematode genera Steinernema and Heterorhabditis are taxonomi-
cally, biologically and commercially the most important ones, as they carry the
lethal bacteria Xenorhabdus spp. and Photorhabdus spp., respectively, which are
responsible for killing insects. The symbiotic nematode–bacteria pairs exhibit a
wide spectrum of insecticidal activity across several economically important orders
of the class Insecta. Considerable success has been achieved under field conditions
in the biocontrol of several pests belonging to the orders Lepidoptera, Coleoptera
and Diptera on high-value commercial crops.
Lepidoptera is second only to Coleoptera in its range of insect diversity, which
includes pests destructive to agricultural, ornamental, landscape and forest vege-
tation. As Steinernema and Heterorhabditis are soil-borne, they attack a wide range
of insect pests, which spend at least a part of their life cycle, particularly the feeding
stages, in the soil. Many lepidopteran species overwinter as the last instar larvae or
pupae in the soil or under fallen leaves and other debris on the soil surface.
Generally, in cryptic habitats, chemical pesticides and biological control agents
other than EPN are unsuccessful in reaching the overwintering stages of larvae or
S. Mohan (*)
Division of Nematology, Indian Agricultural Research Institute, New Delhi 110012, India

DOI 10.1007/978-3-319-14499-3_13
274 S. Mohan
pupae effectively. The foraging strategies of EPN are ideally suited for hunting out
these lepidopteran pests, in their various stages, and their success against
some commercially important species of pests in cryptic habitats has been quite
encouraging.
Glaser’s (1932) path-breaking report on the infection of the Japanese beetle by
Steinernema carpocapsae gave new directions to the control of insect pests. With
the inception of several new species of EPN pathogenic to insects and other
invertebrates, biocontrol of insect pests using nematodes gained credence. The
ease in rearing and handling of lepidopteran pests, such as Galleria, Corcyra,
Bombyx, Plutella, Helicoverpa, Spodoptera, Pieris, Agrotis, etc., has made them
the perfect hosts in laboratory investigations involving biocontrol by nematodes.
These insects have served to effectively illustrate a range of EPN biocontrol
attributes such as their biology, reproduction, in vivo mass production, patho-
genicity and insect immunity.
Notwithstanding the overwhelming efficacy of EPN in laboratory bioassays in
achieving mortality against almost all major lepidopteran pests, the results have not
been replicated at the field level with similar success. For example, the EPN
biocontrol of cutworms—Agrotis, Amathes, Noctua, Peridroma and Prodenia
spp. (Lepidoptera: Noctuidae)—which are voracious feeders on leaf, bud, stem
and roots of several crops and turf has not been successfully implemented on a large
scale in field conditions (Shapiro-Ilan et al. 2002; Ebssa and Koppenhöfer 2011).
However, there have been a few instances of field-level success too as detailed in
Table 13.1.
13.2 Bacterial Symbionts of EPN
Phylogenetically, the two families Steinernematidae and Heterorhabditidae are

dissimilar, but they share a similar life cycle through convergent evolution (Poinar
1993). These nematodes are characterized by their ability to carry specific patho-
genic symbiotic bacteria, Photorhabdus (Boemare et al. 1993) with
Heterorhabditidae and Xenorhabdus (Thomas and Poinar 1979) with
Steinernematidae. The two bacteria belonging to the family Enterobacteriaceae
are medium to long motile rods with peritrichous flagella and are gram-negative
facultative anaerobes. They do not have an environmentally resistant stage and
have been found to exist only as nematode vectors or insect hosts. Xenorhabdus
occurs naturally in a special intestinal vesicle of the Steinernema infective juveniles
(IJ) (Bird and Akhurst 1983), while Photorhabdus is located mainly in the anterior
part of the Heterorhabditis IJ gut (Ciche and Ensign 2003). Both the bacteria are
vectored by their respective nematode hosts into the haemocoel of the insect and
released within 5 h of invasion. They suppress the insect immune response by
producing an array of virulence factors, which eventually kill the host. The bacteria
further contribute to the symbiotic relationship by secreting several exoenzymes
that stimulate macromolecular degradation, the products of which together with the
13 Entomopathogenic Nematodes and Their Bacterial Symbionts as Lethal. . . 275
Table 13.1 Current use of Steinernema and Heterorhabditis nematodes as biological control
organisms (modified from Shapiro-Ilan and Gaugler 2010)
Pest common Efficacious
Crops name Pest scientific name nematodes
Artichokes Artichoke plume Platyptilia carduidactyla Sc
moth
Vegetables Armyworm Lepidoptera: Noctuidae Sc, Sf, Sr
Ornamentals Banana moth Opogona sacchari Hb, Sc
Turf, vegetables Black cutworm Agrotis ipsilon Sc
Fruit trees, Borer Synanthedon spp. and other Hb, Sc, Sf
ornamentals sesiids
Pome fruit Codling moth Cydia pomonella Sc, Sf
Vegetables Corn earworm Helicoverpa zea Sc, Sf, Sr
Cranberries Cranberry girdler Chrysoteuchia topiaria Sc
Grapes Grape root borer Vitacea polistiformis Hz, Hb
Iris Iris borer Macronoctua onusta Hb, Sc
Nut and fruit trees Navel orangeworm Amyelois transitella Sc
1. Nematodes listed provided at least 75 % suppression of these pests in field or greenhouse
experiments
2. Nematode species are abbreviated as follows: Hb H. bacteriophora, Hz H. zealandica, Sc
S. carpocapsae, Sf S. feltiae and Sr S. riobrave
3. Efficacy against various pest species within this group varies among nematode species
bacteria themselves are thought to provide a nutrient-rich base to their nematode

partners (Waterfield et al. 2001; Cowles and Goodrich-Blair 2005; Clarke 2008).
The bacteria proliferate exponentially and produce diverse antimicrobial com-
pounds that inhibit the growth of a wide range of organisms and thus protect the
cadavers from purification (Akhurst 1982; Furgani et al. 2008). The nematodes feed
and reproduce inside the insect cadavers until their growing numbers and
diminishing nutrition compel the IJ to re-associate with the bacteria and emerge
into the soil to look for new hosts (Martens et al. 2003; Snyder et al. 2007).
13.3 Foraging Strategy
The third-stage juvenile or the dauer larva is the infective stage for both
Steinernema and Heterorhabditis. The IJ occur naturally as free-living forms in
the soil and are resistant to the environmental conditions (Poinar and Georgis
1990). They contain carbohydrate energy reserves which help them to survive for
long periods in the soil under unfavourable temperature, moisture and aeration
conditions. The ambient conditions required for survival, infection and reproduc-
tion vary with the nematode species and their natural habitat.
IJ have a unique ability to locate their hosts in both soil and cryptic habitats. An
understanding of this ability is fundamental to arriving at precise EPN–pest
276 S. Mohan
matches and thus enabling effective biocontrol. IJ adopt two modes, ambushing and
cruising (Gaugler et al. 1989), to locate and infect an insect larva. An ambushing
nematode stands upright on its tail, raising more than 95 % of its body off the
substrate; this stance is known as nictation. The nictating nematode attaches to an
insect host passing by it (Campbell et al. 1996). Ambushers target highly mobile
lepidopteran pests active at the soil surface, such as cutworms and armyworms.
Cruisers, as the name implies, are EPN that move through the soil in search of an
insect host. They are most effective against sedentary and slow-moving insect pests
found at various soil depths, such as white grubs and root weevils. Cruisers seek out
their prey by sensing the carbon dioxide or other volatiles released by it:
S. carpocapsae and S. scapterisci are ambushers, and Heterorhabditis
bacteriophora, H. megidis, S. kraussei and S. glaseri are cruisers, while
S. riobrave and S. feltiae are both (Campbell and Gaugler 1997).
13.4 Efficacy of EPN Against Lepidopteran Pests

in Field Conditions
In general, under field conditions, steinernematids have performed better against

lepidopteran pests as compared to heterorhabditids. The ambusher nematode,
S. carpocapsae, has been relatively more successful followed by S. glaseri,
S. feltiae and S. riobrave against several lepidopteran pests causing substantial
damage to orchard trees and field crops. Campbell and Gaugler (1993) reported that
S. carpocapsae was up to 43 times more effective at finding a mobile insect host as
compared to H. bacteriophora. It also tended to search the prey more effectively
along a surface than through a matrix. This section pertains to the successful field
applications of EPN against some significant lepidopteran pests.
13.4.1 Orchard Trees
The control of lepidopteran pests in orchard trees is a major challenge throughout

the world, due to the tendency of the final stage larvae of several species to moult
into cocoon and overwinter in cryptic habitats such as under loose bark and fallen
leaves, in piles of litter at the base of a tree, in woodpiles or even in fruit bins. By
virtue of their foraging strategies and their symbiotic bacterial toxicity, EPN are
ideally equipped to reach, infect and kill those stages of the pests that are concealed
in the cryptic habitat, while chemical pesticides, and fungal, bacterial, or viral
biocontrol agents have a limited reach and lethal capacity.
13.4.1.1 Apple and Pear
Apple and pear trees are attacked by a broad spectrum of lepidopteran pests that
feed on the fruits, leaves and vascular tissues of the trees. The damage caused can
range from huge economic losses incurred due to reduced fruit quality to a total
destruction of the tree.
Apples are one of the most valuable fruit crops in the USA, and the codling
moth, Cydia pomonella, is considered a major constraint, in not only apple orchards
but also in pear, walnut and other fruit trees. The neonates bore into the fruit and
spend their entire feeding time inside it until the last stage larvae leave the fruit in
search of concealed spaces, beneath the bark or under the fallen leaves to pupate.
S. carpocapsae has provided an excellent control of this pest at a dose of 1–
2 106 IJ/tree and the surrounding areas, provided the treated areas are kept moist
for 8 h or more (Lacey and Unruh 1998; Lacey and Chauvin 1999; Unruh and Lacey
2001). Inasmuch as it has been observed that a dry environment is detrimental to the
survival and persistence of the IJ, the active nematodes effectively kill the insect in
its various stages: the final instar larvae after they exit the fruit, the cocooned
prepupae and the cocoon stages that overwinter in the cryptic habitats.
A 4-year field trial was conducted in the apple and pear orchards in eastern
Washington State Field with S. carpocapsae and S. feltiae to determine the effects
of seasonal temperatures, adjuvants, post-application irrigation and the methods of
application in controlling the cocooned codling moth larvae. EPN were applied to
apple trees (Golden Delicious) with a backpack sprayer at the rate of 106 IJ/tree,
supplemented with a wetting agent to aid the survival of the IJ. In September 1999,
a high mortality of 94–95 % was observed in the treatments of both the species.
However, the efficacy of S. carpocapsae reduced to 58 % as against 90 % in
S. feltiae in October, owing to the low temperature. In March, cool windy condi-
tions reduced the efficacy of S. carpocapsae and S. feltiae to 26 % and 65 %,
respectively. Warmer weather in April stepped up the efficacy of S. carpocapsae
and S. feltiae to 71 % and 86 %, respectively. In further tests on the same location,
in mid-October 2001, S. feltiae when combined with a wetting agent (Silwet L77)
or a humectant (STOCKOSORB) (106 IJ/tree) was found to be the most effective
control of the sentinel codling moth larvae, cocooned on cardboard strips (80 %
mortality) and logs (34–47 %). Also posttreatment, the trees were misted (wetted)
for 4 h. The misting of trees, both before and after the application of S. carpocapsae,
has been recommended by Unruh and Lacey (2001) to facilitate the IJ to enter the
cryptic microhabitat of the host and penetrate the cocoon.
Cydia pomonella granulovirus (CpGV) is considered a highly effective and
environmentally benign control agent for the codling moth in organic apple pro-
duction. However, in Germany, this pest reportedly developed resistance against
CpGV leading to an investigation of the potential of EPN in controlling the
populations of the codling moth in organic fruit orchards. In two separate field
tests, treatments of S. glaseri (cruiser) and S. carpocapsae (ambusher) resulted in a
90 % reduction in adult emergence from the treated stems. On-farm trials, under
278 S. Mohan
favourable weather conditions, showed about 50–60 % control of infestation by the

codling moth in the year following the application (Kienzle et al. 2008).
In northern China, the larvae of the peach fruit borer, Carposina niponensis, is a
major pest in apple causing monetary loss of up to 1.7 billion dollars per annum in
1 million ha of orchards. Bedding (1990) reported more than 90 % mortality in the
pest by the application of S. carpocapsae.
13.4.1.2 Nuts
The navel orangeworm, Amyelois transitella Walker, is a major pest of commer-

cially grown almonds, pistachios, figs and walnuts. In California, it poses a signi-
ficant threat to the cultivation of almonds and pistachios, crops with high and
increasing value. The moths oviposit during summer, and the larvae spend the
winter feeding on the nuts left behind on the trees and the ground, post harvest.
Thereafter, they infect the new crop of nuts the following year. Growth regulators
and pyrethroid insecticides have been seen to have limited effect in controlling the
overwintering larvae (Zalom et al. 1984; Bentley et al. 2008). S. carpocapsae was
able to kill >72 % of the navel orangeworm larvae, when applied at a rate of
105 IJ m2 by the manual spraying method (Siegel et al. 2004).
In another application in Ballico, California, S. feltiae Mexican strain was
applied with a backpack Echo duster–mister or a pick-up mounted handgun sprayer
at the rate of 3 106 IJ in 5 l of water per almond tree infected with the navel
orangeworm. Up to 78 % mortality was recorded, and the application schedule was
recommended for small-scale nematode control on almonds (Lindegren
et al. 1987). The IJ were found to remain viable and infective inside the almond
hulls for at least 8–10 days or more.
The filbertworm, Cydia latiferreana, is considered an economically important
insect pest of hazelnuts in North America. S. carpocapsae strain All was used to
manage the overwintering worms on the hazelnut orchard floor. The filbertworm
larvae were treated with the nematode at 40–150 IJ/cm2 applied in 75 or 190 ml/m2
water, in plots with either bare soil or litter (leaves, twigs, husks and blank nuts).
This resulted in a 2–11 % reduction in their population in October 2007 and at
50–78 % in May 2008 (Chambers et al. 2010).
13.4.1.3 Peach
The lesser peach tree borer, Synanthedon pictipes Grote and Robinson, a pest of
commercially grown Prunus spp., is indigenous to eastern North America, espe-
cially in the southeastern peach orchards. The larvae treated with four strains of
S. carpocapsae (All, DD136, Sal and Hybrid2) resulted in <20 % survival, whereas
larval survival was always >50 % when treated with three strains of S. riobrave
(3-8b, 7-12 and 355) or H. spp. Due to the simultaneous occurrence of overlapping
generations of S. pictipes larvae in the orchards, the susceptibility of the larvae
could also be mapped versus their size: large and medium larvae were more
susceptible than the small ones. The above-ground application of these nematodes
was more efficacious due to the moisture-retaining covers placed over S. pictipes-
infested wounds on the peach limbs (Cottrell et al. 2011).
In China, the peach fruit moth, Carposina niponensis, which pupates in the soil,
has been effectively controlled by S. carpocapsae (Wang 1993).
13.4.1.4 Strawberry
The S. carpocapsae strain Agriotos and the H. bacteriophora strain Oswego were
extremely virulent to the last instar larvae of the strawberry crown moth,
Synanthedon bibionipennis, even in the protected environment inside the straw-
berry crown, causing 96 % and 94 % mortality, respectively, in the laboratory.
However, field applications in late fall (October) were less effective with
S. carpocapsae and H. bacteriophora, resulting in 51 % and 33 % infection,
respectively (Bruck et al. 2008). It was recommended that to improve field-level
control, nematode applications should be made in late summer to early fall when
larvae are present in the soil, and the soil temperatures are more favourable for
nematode infection.
13.4.1.5 Grape
The grape root borer, Vitacea polistiformis Harris, is the most important insect pest
of grapes in Florida. It is a difficult pest to detect and control because it spends the
majority of its life cycle underground. The larvae sequester the grape roots, and thus
the damage is not readily visible. The exposed roots of an infested vine show
tunnels just under the cambium filled with a reddish frass and trunk girdling. An
application of 5 billion/ha of H. zealandica achieved 70 % control of the borers.
In another study, an application of 60,000 H. zealandica IJ/plant caused 96 %
mortality, as the IJ could reach the borer even in the root pieces (Pollock 2002).
Saunders and All (1985) found that S. carpocapsae burrowed down to the primary
feeding sites of the borer in the roots and effectively killed the first larval instars.
Gray and Johnson (1983) also found S. carpocapsae to be most effective in
suppressing the borer activity at 29 C, and their survival was directly correlated
to the soil temperatures (30 C) and soil moisture (>79.5 % RH) at which IJ lived
for up to 1.5–2 years.
13.4.1.6 Litchi
S. carpocapsae was found to be highly lethal to the litchi stem borer, Indarbela dea,
causing over 86 % mortality by spraying 1,000 IJ around the borer hole, where the
larvae are usually active during night (Xu and Yang 1992).
280 S. Mohan
13.4.1.7 Alder and Sycamore
The larvae of the clear-wing moth borer, Synanthedon spp., bore into plant tissues
and feed in the galleries made in the barks and roots of trees, resulting in wood
defects or structural weakness that may eventually destroy the tree. S. culiciformis,
which bores into the heartwood of alder trees, was sprayed with S. feltiae with a
Hudson sprayer at the rate of 6.5 or 11.5 106 IJ/tree or with a 1-pint squirt bottle at
the rate of 18,000 or 36,000 IJ/gallery, providing 17–84 % and 86–93 % borer
control, respectively, in Davis California. S. resplendens, a bark borer of sycamore
trees, treated with 11.3 106 S. feltiae IJ/tree or 8.6 106 S. bibionis IJ/tree in
Riverside, California, resulted in 61 % and 13 % population reduction, respectively
(Kaya and Brown 1986). As S. culiciformis occurs in the moist heartwood, it makes
a large gallery opening (an average of 0.28 in.), thus allowing nematodes to enter or
be sprayed directly into the gallery. Therefore, the moist galleries enhanced the
nematode survival and host-finding ability in alders. A comparatively poor mortal-
ity of borers in sycamore was attributed primarily to the drier galleries in the bark
and the smaller size of the gallery openings (average of 0.05 in. in October and
0.17 in. in April).
13.4.2 Vegetables
In 1996 and 1999, two studies were carried out, at the experimental farm of
Agriculture and Agri-Food Canada at L’Acadie, on the foliar application of
S. carpocapsae. In 1996, S. carpocapsae, applied at 4 billion/ha, provided 35.3 %
control of Artogeia rapae on Brussels sprouts and 33.0 % on broccoli, while the
application of the same in 1999 resulted in 24.9 %, 19.4 % and 14.9 % control on
Brussels sprouts, broccoli and cauliflower, respectively. Based on these field
results, it was concluded that foliar applications of S. carpocapsae did not provide
an acceptable level of Artogeia rapae control under Quebec’s environmental
conditions (Bélair et al. 2003).
Field inundation of S. feltiae strain All, S. bacteriophora strain Hp88 and
H. heliothidis strain NC to control the larvae of the banana moth, Opogona sacchari
Bojer, infesting potato and bamboo palms in Florida resulted in successful esta-
blishment of the nematodes and a 58–100 % reduction in the pest population.
The residual of H. heliothidis strain NC reduced the new infestations more effec-
tively than the residual of S. feltiae strain All, as the latter had a lower survival rate
(Pena et al. 1990).
In India, field trials conducted between 2002 and 2004 to control diamondback
moth, Plutella xylostella, infestation of cabbage through the foliar application of
S. thermophilum caused up to 46 % mortality in the larvae. EPN treatment reduced
crop damage up to 43.1 % as compared to 49.5 % in treating with the insecticide
Lambda-cyhalothrin (Somvanshi et al. 2006).
13.4.3 Corn
S. riobrave was found to be highly effective as inundative control against the

prepupae and pupae of the corn earworm, Helicoverpa zea, in the cornfields of
Texas, under conditions of high soil temperature, optimal irrigation and appropriate
time of application (Cabanillas and Raulston 1995, 1996). It was also successful
against this pest in the Arkansas cornfields (Feaster and Steinkraus 1996).
13.4.4 Cotton
Cotton, the most important natural fibre crop in the world, harbours a large number
of insect pests that are susceptible to EPN. An application of S. riobrave at
2.5 billion IJ/ha in cotton fields in Arizona heavily infested with the pink bollworm,
Pectinophora gossypiella, showed a reduction in infested bolls and a 19 % increase
in the yield as compared to the untreated fields. The nematodes persisted in large
numbers for 19 days and were recovered up to 75 days after application (Gouge
et al. 1996), while similar results were obtained with S. riobrave and S. carpocapsae
in the cotton fields in Texas (Gouge et al. 1997).
13.4.5 Turf
The black cutworm, Agrotis ipsilon, a polyphagous pest, is a perennial problem of

golf course greens. The larvae spend their life cycle in the soil and are susceptible to
a number of EPN. Georgis and Poinar (1994) reported 95 % control by
S. carpocapsae. Buhler and Gibb (1994) observed that while both S. carpocapsae
and S. glaseri significantly reduced the cutworm larvae in creeping bentgrass,
S. carpocapsae provided a slightly higher level of control than S. glaseri. However,
the persistence of nematodes was lost 8 days after their application in the field.
13.4.6 Management of Pests in Fruit Bins
The propagation of the codling moth larvae, Cydia pomonella, as a diapausing stage
in fruit bins, is a potential source of reinfestation of orchards. The immersion of the
infested bins in suspensions of commercially produced nematodes ranging from
10 to 50 IJ/ml water, along with a wetting agent and a humectant, resulted in 45–
87 % and 56–85 % mortality in the cocooned codling moth larvae for S. feltiae and
S. carpocapsae, respectively (Lacey et al. 2005). The wetting agent, Silwet L77,
282 S. Mohan
was used to assist the penetration of EPN into the codling moth hibernacula and the
humectant to prevent EPN desiccation.
In another study, the diapausing cocooned oriental fruit moth, Grapholita
molesta Busck, larvae in fruit bins were found to be susceptible to the IJ of
S. feltiae. The treatment of bins with suspensions of 10 or 25 S. feltiae IJ/ml
water along with the wetting agent, Silwet L77, resulted in 33.3–59 % and 77.7–
81.6 % mortality in the corner supports and cardboard strips, respectively (Riga
et al. 2006).
13.4.7 EPN in Combination with Bt
To improve the efficiency and efficacy of EPN, several attempts were made to
combine them with other bioagents. The application of Bacillus thuringiensis (Bt)
and S. carpocapsae, both at half rate, to control P. xylostella on watercress resulted
in 58 % control, which was significantly higher than that of the individual appli-
cation of each at full rate (Baur et al. 1998). However, the use of additives to
improve EPN persistence and efficacy could not increase the feasibility of foliar
applications against P. xylostella on watercress (Baur et al. 1997). The combination
of S. carpocapsae and Bt with a polymer, by Schroer and Ehlers (2005) and Schroer
et al. (2005), provided similar results. The application of 0.5 million/m2 of
S. carpocapsae, along with a surfactant–polymer formulation achieved a significant
reduction of P. xylostella per plant with >50 % control after 7 days and 45 %
control after 14 days in cabbage cultivated in east Java and Indonesia. Weekly
applications of Bt (TUREX®) or its alternate application with the nematodes
achieved >80 % control. The application of both biological agents together every
second week reached insignificant lower efficacy (70 %). The success of the
nematodes in these trials could mainly be attributed to the high humidity in the
experimental area.
The moist microhabitat and moderate temperatures during the artichoke-
growing season in the fog belt region of the central coast of California were
found to be ideal for the management of the artichoke plume moth, Platyptilia
carduidactyla, which typically tunnels young vegetative shoots. Field application
of Neoaplectana carpocapsae (¼S. feltiae) Weiser at 2,000 IJ/ml gave 100 %
control of the third and fourth instars, 15 days after treatment. Its residual effect,
evaluated 24 days after treatment, was greater than that of a commonly used
insecticide, methidathion. Bt var. kurstaki at 19.6 billion international units/ha
gave significant larval control; its efficacy was not significantly lower than that of
methidathion. A combination of the nematode and Bt var. kurstaki did not result in
significantly greater control than that achieved by the nematode used alone at
1,000 IJ/ml (Barp and Kaya 1984).
13.4.7.1 Incorporation of EPN in Bt Refuge
The evolution of resistance by pests can reduce the efficacy of Bt transgenic crops.
In conjunction with refuges of non-Bt host plants, fitness costs can delay the
evolution of resistance and thereby prolong the efficacy of Bt crops. Baur
et al. (1998) had suggested that EPN could serve as components of integrated
pest management of P. xylostella and could help manage resistance to Bt.
Gassmann et al. (2006) reiterated that EPN could increase the fitness costs of
resistance to Bt toxin. Later, Gassmann et al. (2008) tested the effects of
S. riobrave on insect mortality and fitness costs of resistance to Bt toxin Cry1Ac
in the pink bollworm Pectinophora gossypiella Saunders, a major pest of cotton in
the southwestern United States. The results indicated that EPN could act synergis-
tically with Bt crops by killing the pests in non-Bt refuges and could delay
resistance by pests to Bt crops. However, no effect on fitness costs was detected
for the nematode H. bacteriophora. Hannon et al. (2010) concluded that EPN could
bolster insect resistance management, but the success of this approach would
depend on the selection of the appropriate species of nematode and the
environment.
13.5 Symbiotic Bacteria as Biopesticide
Both Xenorhabdus and Photorhabdus can be grown as free-living organisms under

standard laboratory conditions on growth media. They are known to secrete several
extracellular products, including lipase, protease and lipopolysaccharides, and
many broad spectrum antibiotics in the culture medium (Akhurst 1982; Forst
et al. 1997). Bacterial proteins that are effective insecticides include crystal proteins
(δ endotoxins) of Bt and Bacillus sphaericus, the Vip toxins of Bt and cholesterol
oxidase of Streptomyces spp., all of which lyse the midgut epithelium of the insect
host (Bowen et al. 1998). Similar insecticidal properties have been found in
Photorhabdus luminescens and Xenorhabdus nematophilus. Most of the bacterial
strains have been found to be highly toxic when injected, and a few display lethal
oral toxicity as well. Recently, a 51.8 kDa protease, purified from P. luminescens
strain 0805-P5G, showed high insecticidal activity in G. mellonella when injected,
and it also showed high oral toxicity in the P. xylostella of a Taiwan field-collected
strain, but low toxicity in an American strain (Chang et al. 2013).
The insecticidal toxin proteins secreted by Photorhabdus, during its growth, into
the culture medium, have been purified (Bowen and Ensign 1998) and their genes
cloned (Bowen et al. 1998). Such reports have influenced the utilization of these
bacteria as biopesticides independent of their nematode hosts. A patent for the use
of Xenorhabdus nematophilus against fire ants has been issued (Dudney 1997).
Direct toxicity of P. luminescens to insects under natural conditions was demon-
strated by the author for the first time. Within 24 h of foliar application of
284 S. Mohan
P. luminescens, 100 % of the cabbage white butterfly Pieris brassicae larvae

naturally infesting the Nasturtium fields were killed (Mohan et al. 2003). Foliar
application of P. luminescens and X. nematophilus resulted in 60 % and 40 %
mortality to the pupae of P. xylostella, respectively (Razek 2003).
Actively growing cells of P. luminescens (2.5 107/bead) encapsulated in
sodium alginate and mixed with sterilized soil, when exposed to Spodoptera litura
larvae, resulted in 100 % mortality in 48 h, while the use of alginate-encapsulated
H. indica effected 40 % mortality after 72 h. The LC50 of Photorhabdus cells was
estimated at 1,010 cells per larva for killing the S. litura 6th instar larvae in 48 h
(Rajagopal et al. 2006).
In the wake of such advances, P. luminescens and its formulations are being
explored for inclusion in pest management strategies. However, their wide-ranging
entomopathogenicity gives rise to doubts regarding their ecological safety against
beneficial/nontarget fauna in general and insects in particular. The ecological
compatibility of P. luminescens cells or their secreted toxins against the hatching
of two species, Trichogramma chilonis Ishii and T. japonicum Ashmead, of a
commercially recommended biological control agent on the hyperparasitized eggs
of the stored grain moth Corcyra cephalonica was studied in vitro. In 65 % of the
eggs exposed to P. luminescens cells alone or their toxins, the Corcyra eggshells
became flaccid, and there was up to 84 % reduction in the emergence of the
Trichogramma adults (Mohan and Sabir 2005). Such results point to the
bioecological hazards of indiscriminate use of P. luminescens as a biopesticide.
Thus, due to its wide host range, the inclusion of P. luminescens in any integrated
pest management programme would be suspected, until proven safe for natural
enemies and nontarget organisms.
13.6 Conclusion
Despite the successes achieved in field trials, a more promising future for the EPN
would be to optimize their efficacy under location-specific sites. It is crucial to
identify and select more pathogenic native nematode species and strains, which can
adapt to the ecology and biology of the lepidopteran host insect. An optimal
combination of a nematode species and its dosage vis-a-vis a specific insect pest
is imperative for field-level success of EPN. A fundamental understanding of the
interaction between indigenous EPN species and isolates with the susceptible stages
of the pest is important.
The contention of the biosafety of the symbiotic bacteria to beneficial organisms
needs to be examined more critically as these organisms are ecologically obligate to
EPN, with specific mechanisms of pathogenicity, and their existence in free form in
nature is believed to last a very short while due to photo- and thermal sensitivity.
References
Akhurst RJ (1982) Antibiotic activity of Xenorhabdus spp., bacteria symbiotically associated with
insect pathogenic nematodes of the families Heterorhabditidae and Steinernematidae. J Gen
Microbiol 128:3061–3065
Barp MA, Kaya HK (1984) Evaluation of the entomogenous nematode Neoaplectana carpocapsae
(¼S. feltiae) Weiser (Rhabditida: S.tidae) and the bacterium Bacillus thuringiensis Berliner
var. kurstaki for suppression of the Artichoke Plume Moth (Lepidoptera: Pterophoridae).
J Econ Entomol 77:225–229
Baur ME, Kaya HK, Gaugler R, Tabashnik B (1997) Effects of adjuvants on entomopathogenic
nematode persistence and efficacy against Plutella xylostella. Biocontrol Sci Technol 7:
513–525
Baur ME, Kaya HK, Tabashnik BE, Chilcutt CF (1998) Suppression of diamondback moth
(Lepidoptera: Plutellidae) with an entomopathogenic nematode (Rhabditida: Steinernematidae)
and Bacillus thuringiensis Berliner. J Econ Entomol 91:1089–1095
Bedding RA (1990) Logistic and strategies for introducing entomopathogenic nematode technol-
ogy into developing countries. In: Gaugler R, Kaya HK (eds) Entomopathogenic nematodes in
biological control. CRC Press, Boca Raton, pp 233–246
Bélair G, Fournier Y, Dauphinais N (2003) Efficacy of steinernematid nematodes against three
insect pests of crucifers in Quebec1. J Nematol 35:259–265
Bentley W, Siegel JP, Holtz BA, Daane KM (2008) Navel orangeworm (Amyelois transitella)
(Walker) and obliquebanded leafroller (Choristoneura rosaceana) (Harris) as pests of pista-
chio. In: Ferguson LF, Beede RH, Haviland DH, Holtz BA, Kallsen CE, Sanden BL (eds)
Pistachio production manual. University of California, Davis, pp 179–191
Bird AF, Akhurst RJ (1983) The nature of the intestinal vesicle in nematodes of the family
Steinernematidae. Int J Parasitol 13:599–606
Boemare NE, Akhurst RJ, Mourant RG (1993) DNA relatedness between Xenorhabdus species, a
symbiotic bacteria from entomopathogenic nematodes, and a proposal to transfer Xenorhabdus
luminescens to a new genus Photorhabdus gen. nov. Int J Syst Bacteriol 43:249–255
Bowen DJ, Ensign JC (1998) Purification and characterization of a high-molecular-weight insecti-
cidal protein complex produced by the entomopathogenic bacterium Photorhabdus luminescens.
Bowen DJ, Blackburn RM, Andreev O, Golubeva E, Bhartia R, ffrench-Constant RH (1998)
Insecticidal toxins from bacterium Photorhabdus luminescens. Science 280:2129–2132
Bruck DJ, Edwards DL, Donahue KM (2008) Susceptibility of the strawberry crown moth
(Lepidoptera: Sesiidae) to entomopathogenic nematodes. J Econ Entomol 101:251–255
Buhler WG, Gibb TJ (1994) Persistence of S. carpocapsae and S. glaseri as measured by their
control of black cutworm larvae in bentgrass. J Econ Entomol 87:638–642
Cabanillas HE, Raulston JR (1995) Impact of S. riobravis (Rhabditida: Steinernematidae) on the
control of Helicoverpa zea (Lepidoptera: Noctuidae) in corn. J Econ Entomol 88:58–64
Cabanillas HE, Raulston JR (1996) Evaluation of S. riobravis, S. carpocapsae, and irrigation
timing for the control of corn earworm, Helicoverpa zea. J Nematol 28:75–82
Campbell JF, Gaugler R (1993) Nictation behaviour and its ecological implications in the host
search strategies of entomopathogenic nematodes. Behaviour 126:155–169
Campbell JF, Gaugler R (1997) Inter-specific variation in entomopathogenic nematode foraging
strategy: dichotomy or variation along a continuum? Fund Appl Nematol 20:393–398
Campbell J, Lewis E, Yoder F, Gaugler R (1996) Entomopathogenic nematode spatial distribution
in turfgrass. Parasitology 113:473–482
Chambers U, Bruck DJ, Olsen J, Walton VM (2010) Control of overwintering filbertworm
(Lepidoptera: Tortricidae) larvae with S. carpocapsae. J Econ Entomol 103:416–422
Chang YT, Hsieh C, Wu LC, Chang HC, Kao SS, Meng M, Hsieh FC (2013) Purification and
properties of an insecticidal metalloprotease produced by Photorhabdus luminescens strain
0805-P5G, the entomopathogenic nematode symbiont. Int J Mol Sci 14:308–321
286 S. Mohan
Ciche TA, Ensign JC (2003) For the insect pathogen Photorhabdus luminescens, which end of a
nematode is out? Appl Environ Microbiol 69:1890–1897
Clarke DJ (2008) Photorhabdus: a model for the analysis of pathogenicity and mutualism.
Cell Microbiol 10:2159–2167
Cottrell TE, Shapiro-Ilan DI, Horton DL, Mizell RF (2011) Laboratory virulence and orchard
efficacy of entomopathogenic nematodes against the lesser peachtree borer (Lepidoptera:
Sesiidae). J Econ Entomol 104:47–53
Cowles KN, Goodrich-Blair H (2005) Expression and activity of a Xenorhabdus nematophila
haemolysin required for full virulence towards Manduca sexta insects. Cell Microbiol 7:
209–219
Dudney RA (1997) Use of Xenorhabdus nematophilus Im/1 and 19061/1 for fire ant control.
US Patent US 5,616,318 970, 401. Application Information US 95-488,820 950,609
Ebssa L, Koppenhöfer AM (2011) Efficacy and persistence of entomopathogenic nematodes for
black cutworm control in turfgrass. Biocontrol Sci Technol 21:779–796
Feaster MA, Steinkraus DC (1996) Inundative biological control of Helicoverpa zea (Lepidoptera:
Noctuidae) with the entomopathogenic nematode S. riobravis (Rhabditida: Steinernematidae).
Forst S, Dowds B, Boemare N, Stackebrandt E (1997) Xenorhabdus and Photorhabdus spp.:
bugs that kill bugs. Annu Rev Microbiol 51:47–72
Furgani G, Boszormenyi E, Fodor A, Mathe-Fodor A, Forst S (2008) Xenorhabdus antibiotics: a
comparative analysis and potential utility for controlling mastitis caused by bacteria. J Appl
Gassmann AJ, Stock SP, Carrière Y, Tabashnik BE (2006) Effect of entomopathogenic nematodes
on the fitness cost of resistance to Bt toxin Cry1Ac in pink bollworm (Lepidoptera:
Gelechiidae). J Econ Entomol 99:920–926
Gassmann AJ, Stock SP, Sisterson MS, Carrière Y, Tabashnik BE (2008) Synergism between
entomopathogenic nematodes and Bacillus thuringiensis crops: integrating biological control
and resistance management. J Appl Ecol 45:957–966
Gaugler R, Campbell J, McGuire T (1989) Selection for host finding in S. feltiae. J Invertebr Pathol
54:363–372
Georgis R, Poinar GO Jr (1994) Nematodes as bioinsecticides in turf and ornamentals.
In: Leslie AR (ed) Integrated pest management of turfgrass and ornamentals. Lewis Publishers,
Boca Raton, pp 477–489
Glaser RW (1932) Studies on Neoaplectana glaseri, nematode parasite of the Japanese beetle
(Popillia japonica). N J Dept Agric 211:34
Gouge DH, Reaves LL, Stoltman MM, Van Berkum JR, Burke RA, Forlow Jech LJ, Henneberry
TJ (1996) Control of Pink boll-worm Pectiniphora gossipiella (Saunders) larvae in Arizona
and Texas cotton fields using entomopathogenic nematodes S. riobravis. In: Ritcher DA,
Armour J (eds) Proceedings of the Beltwide cotton production research conference.
National Cotton Council of America, Memphis, pp 1078–1082
Gouge DH, Smith KA, Payne C, Lee LL, Van Berkum JR, Ortega D, Henneberry, TJ (1997)
Control of Pink boll-worm Pectiniphora gossipiella (Saunders) with biocontrol and biorational
agents. In: Dugger P, Ritcher DA (eds) Proceedings of the Beltwide cotton production research
conference. National Cotton Council of America, Memphis, pp 1066–1072
Gray PA, Johnson DT (1983) Survival of the nematode Neoaplectana carpocapsae in relation to
soil temperature, moisture, and time. J Georgia Entomol Soc 18:454–460
Hannon ER, Sisterson MS, Stock SP, Carrière Y, Tabashnik BE, Gassmann AJ (2010) Effects of
four nematode species on fitness costs of pink bollworm resistance to Bacillus thuringiensis
toxin Cry1Ac. J Econ Entomol 103:1821–1831
Kaya HK, Brown LR (1986) Field application of entomogenous nematodes for biological control
of clear-wing moth borers in alders and sycamore trees. J Arboric 12:150–154
Kienzle J, Zimmer J, Volk F, Zebitz CPW (2008) Experiences with entomopathogenic nematodes
for the control of overwintering codling moth larvae in Germany. Archived at http://orgprints.
org/13712
Koppenhöfer AM (2007) Nematodes. In: Lacey LA, Kaya HK (eds) Field manual of techniques in
invertebrate pathology: application and evaluation of pathogens for control of insects and other
invertebrate pests, 2nd edn. Springer, Dordrecht, pp 249–264
Lacey LA, Chauvin RL (1999) Entomopathogenic nematodes for control of codling moth in
fruit bins. J Econ Entomol 92:104–109
Lacey LA, Unruh TR (1998) Entomopathogenic nematodes for control of codling moth, Cydia
pomonella (Lepidoptera: Tortricidae): effect of nematode species, concentration, temperature,
and humidity. Biol Control 13:190–197
Lacey LA, Neven LG, Headrick HL, Fritts R Jr (2005) Factors affecting entomopathogenic
nematodes (Steinernematidae) for control of overwintering codling moth (Lepidoptera:
Tortricidae) in fruit bins. J Econ Entomol 98:1863–1869
Lindegren JE, Agudelo-Silva F, Valero KA, Curtis CE (1987) Comparative small scale field
application of S. feltiae for navel orangeworm control. J Nematol 19:503–504
Martens EC, Heungens K, Goodrich-Blair H (2003) Early colonization events in the mutualistic
association between S. carpocapsae nematodes and Xenorhabdus nematophila bacteria.
J Bacteriol 185:3147–3154
Mohan S, Sabir N (2005) Biosafety concerns on the use of Photorhabdus luminescens as
biopesticide: experimental evidence of mortality in egg parasitoid Trichogramma spp.
Curr Sci 89:1268–1272
Mohan S, Raman R, Gaur HS (2003) Foliar application of Photorhabdus luminescens, symbiotic
bacteria from entomopathogenic nematode H. indica, to kill cabbage butterfly Pieris brassicae.
Curr Sci 84:1397
Pena JE, Schroeder WJ, Osborne LS (1990) Use of entomogenous nematodes of the families
Heterorhabditidae and Steinernematidae to control banana moth (Opogona sachari).
Nematropica 20:51–55
Poinar GO Jr (1993) Taxonomy and biology of Steinernematidae and Heterorhabditidae. In:
Gaugler R, Kaya HK (eds) Entomopathogenic nematodes in biological control. Marcel Dekker,
Poinar GO Jr, Georgis R (1990) Characterization and field application of H. bacteriophora strain
HP88. Revue-de-Nematologie 13:387–393
Pollock C (2002) Nematodes effective against grape pests. http://fusion.ag.ohio state.edu/news/
story.asp. Accessed 18 Feb 2003
Rajagopal R, Mohan S, Bhatnagar RK (2006) Direct infection of Spodoptera litura by
Photorhabdus luminescens encapsulated in alginate beads. J Invertebr Pathol 93:50–53
Razek AAS (2003) Pathogenic effects of Xenorhabdus nematophilus and Photorhabdus
luminescens against pupae of the Diamondback moth, Plutella xylostella. J Pest Sci 76:
108–111
Riga E, Lacey LA, Guerra N, Headrick HL (2006) Control of the oriental fruit moth, Grapholita
molesta, using entomopathogenic nematodes in laboratory and fruit bin assays. J Nematol 38:
168–171
Saunders MC, All JN (1985) Association of entomophilic rhabditoid nematode populations with
natural control of first-instar larvae of the grape root borer, Vitacea polistiformis, in Concord
grape vineyards. J Invertebr Pathol 45:147–151
Schroer S, Ehlers RU (2005) Foliar application of the entomopathogenic nematode S. carpocapsae
for biological control of diamondback moth larvae (Plutella xylostella). Biol Control 33:81–86
Schroer S, Sulistydanto D, Ehlers RU (2005) Control of Plutella xylostella using polymer-
formulated S. carpocapsae and Bacillus thuringiensis in cabbage fields. J Appl Entomol 129:
128–204
288 S. Mohan
Shapiro-Ilan DI, Gaugler R (2010) Nematodes: Rhabditida: Steinernematidae & Heterorhabditidae.

In: Shelton A (ed) Biological control: a guide to natural enemies in North America.
Cornell University. http://www.biocontrol.entomology.cornell.edu/pathogens/nematodes.htm
Shapiro-Ilan DI, Gouge DH, Koppenho¨fer AM (2002) Factors affecting commercial success:
case studies in cotton, turf and citrus. In: Gaugler R (ed) Entomopathogenic nematology.
CABI Publishing, Wallingford, pp 333–355
Siegel J, Lacey LA, Fritts R, Higbee BS, Noble P (2004) Use of Steinernematid nematodes for post
harvest control of navel orangeworm (Lepidoptera: Pyralidae, Amyelois transitella) in fallen
pistachios. Biol Cont 30:410–417
Snyder H, Stock SP, Kim SK, Flores-Lara Y, Forst S (2007) New insights into the colonization and
release processes of Xenorhabdus nematophila and the morphology and ultrastructure of the
bacterial receptacle of its nematode host, S. carpocapsae. Appl Environ Microbiol 73:
5338–5346
Somvanshi VS, Ganguly S, Paul AVN (2006) Field efficacy of the entomopathogenic nematode
S. thermophilum Ganguly and Singh (Rhabditida: Steinernematidae) against diamondback
moth (Plutella xylostella L.) infesting cabbage. Biol Control 37:9–15
Thomas GM, Poinar GO Jr (1979) Amended description of the genus Xenorhabdus Thomas and
Poinar. Int J Syst Bacteriol 33:878–879
Unruh TR, Lacey LA (2001) Control of codling moth, Cydia pomonella (Lepidoptera: Tortricidae)
with S. carpocapsae: effects of supplemental wetting and pupation site on infection rate.
Wang J (1993) Control of the peach fruit moth, Carposina niponensis, using entomopathogenic
nematodes. In: Bedding R, Akhurst R, Kaya H (eds) Nematodes and the biological control of
insect pests. CSIRO Publications, East Melbourne, pp 59–65
Waterfield N, Bowen DJ, Fetherston JD, Perry RD, ffrench-Constant RH (2001) The toxin
complex genes of Photorhabdus: a growing gene family. Trends Microbiol 9:185–191
Xu JL, Yang P (1992) The application of the codling moth nematode against the litchi stemborer.
Acta Phytophylacica Sinica 19:217–222
Zalom FG, Barnett WW, Weakley CV (1984) Efficacy of winter sanitation for managing the navel
orangeworm, Paramyelois transitella (Walker), in California almond orchards. Prot Ecol 7:
37–41
Chapter 14
The Management of Helicoverpa Species
by Entomopathogenic Nematodes
M. Abid Hussain and Wasim Ahmad
14.1 Introduction
Helicoverpa spp. (Lepidoptera: Noctuidae) are known by many common names

such as American bollworm, cotton bollworm, corn earworm, tobacco budworm,
old world bollworm, legume/gram pod borer, etc. It is widely distributed in Asia,
Africa, Australia, the Mediterranean Europe and the semiarid tropical regions of the
world. It is a polyphagous pest of many agricultural, horticultural and ornamental
crops (Reed and Pawar 1982; Zalucki et al. 1986; Fitt 1989; Sharma 2001). The
geographic distribution of the Heliothine lepidopteran species complex
(Helicoverpa virescens, H. armigera, H. punctigera and H. zea) is listed in
Table 14.1. The status of Helicoverpa as a serious pest is due to its high mobility,
polyphagy, facultative diapause as pupae, rapid generation turnover, fecundity and
predilection for harvestable parts of high-value crops such as cotton, tomato, pulses
and cereals (Fitt 1989). All of these life-history features contribute to make
Helicoverpa spp. one of the world’s worst pest.
The estimates of yield losses due to this pest vary from 5 to 10 % in the
temperate regions and 50–100 % in the tropics (van Emden et al. 1988). The
avoidable losses in food legumes at production levels of 60.45 million tonnes are
predicted to be nearly 18.14 million tonnes (at an average loss of 30 %), valued at
nearly US$10 billion (Sharma et al. 2008). Barwale et al. (2004) estimated about
Rs. 1,200 crore (equivalent to US$273 million at exchange rate in 2004) worth of
pesticides used in India to control the bollworm complex of cotton, whereas
Helicoverpa causes an estimated loss of US$927 million in chickpea and pigeon
M.A. Hussain
Department of Plant Protection, Hamelmalo Agricultural College, Keren, Eritrea
W. Ahmad (*)
Section of Nematology, Department of Zoology, Aligarh Muslim University, Aligarh 202002,
India

DOI 10.1007/978-3-319-14499-3_14
290 M.A. Hussain and W. Ahmad
Table 14.1 Geographic distribution of the Helicoverpa species complex (Deguine et al. 2008)
Species Geographic distribution Main host plants
Helicoverpa Africa, Central and Southeastern Asia, Cotton, groundnut, maize, pulses,
armigera Australia, Southern Europe, India, rapeseed, safflower, sorghum, soy-
New Zealand and many eastern Pacific bean, sunflower, tobacco, tomato, etc.
Islands, Brazila
Helicoverpa North and South America Cotton, maize, sorghum, soybean,
zea sunflower, tomato
Helicoverpa Australia Chickpea, cotton, lucerne, safflower,
punctigera soybean, sunflower
Helicoverpa North and South America Cotton, soybean, sunflower, tobacco,
virescens sunflower
a
Tay et al. (2013)
pea, and more than US$5 billion on different crops worldwide (Sharma 2005).
Several factors, including increasing levels of resistance to pesticides and rise in
cropping intensity, have contributed to greater importance of this pest (Shanower
et al. 1998). Agronomic factors, such as high-yielding varieties, increased use of
irrigation and fertilisers and large-scale production and planting of alternate crop
hosts, contribute towards greater prevalence and increased severity (Reed and
Pawar 1982; Fitt 1989).
The fundamental of effective integrated pest management (IPM) programmes is
the development of appropriate pest management strategies and tactics that best
interface with the cropping system and the pest situations. The potential of some of
the control tactics to reduce the population density of Helicoverpa spp. in different
cropping systems is evaluated by several researchers. Attempts have been made to
develop integrated management approach for Helicoverpa spp. using host–plant
resistance (Mihm 1997; Sharma et al. 2005; Sharma 2007; Kumari et al. 2010;
Naseri et al. 2010; Soleimannejad et al. 2010) including transgenic Bt crops
(Mendelsohn et al. 2003; Gopala Swamy et al. 2009; Sanahuja et al. 2011; James
2012; Acharjee and Sarmah 2013), biological control (predators and parasitoids)
(King and Coleman 1989; Sharma 2001), interference methods including sex
pheromones for population monitoring or mating disruptions (Reddy and
Manjunatha 2000), biopesticides (especially commercial formulations of Bacillus
thuringiensis) (Navon 2000; Liao et al. 2002; Sanahuja et al. 2011), cultural
practices (including appropriate crop rotations, trap crops, planting date and habitat
complexity) (Jallow et al. 2004) and chemical control (COPR 1983; Zalucki
et al. 1986; Matthews 1989; Jackson 2014). Another IPM tool is push-pull strategy
which can also be deployed to reduce pesticide input (Cook et al. 2007). Neverthe-
less, the pest become serious with regular outbreaks and developed resistance to
almost all conventional insecticides including synthetic pyrethroids (Armes
et al. 1996; Kranthi et al. 2002; Whalon et al. 2007). As a result, chemical control
through the use of synthetic insecticides could not become panacea in the protection
of agriculturally important crops. There are recent claims of field-evolved resis-
tance to proteins in transgenic toxin Bt crops. For example, a survey during 2010
14 The Management of Helicoverpa Species by Entomopathogenic Nematodes 291
revealed field-evolved resistance in H. armigera to Cry1Ac protein in northern

China (Zhang et al. 2011). Yet in another study in Pakistan, the development of
580-fold resistance ratio to Bt toxin Cry1Ac has been reported and compared with
several conventional insecticides (Alvi et al. 2012). Five-year data set of Downes
et al. (2010) shows a significant exponential increase in the frequency of alleles
conferring Cry2Ab Bt toxin resistance in Australian field populations of
H. punctigera.
The use of microbial pesticides has been considered a more sustainable and
environmentally benign option to control Helicoverpa (King and Coleman 1989).
Unfortunately, previous control attempts using entomopathogens were not effective
to a great extent, and substantial reduction in degree of losses caused by this
dreaded pest could not be achieved (Sachan and Lal 1997). The deactivation by
UV light, insufficient titres ingested by larvae and lack of virulence could be the
practical problems. Environmentally safe techniques such as the release of
Trichogramma egg parasitoids, the use of B. thuringiensis (Bt) sprays,
H. armigera nucleopolyhedrovirus (HearSNPV) and sex pheromones are not yet
readily available in rural areas or are relatively less effective than the synthetic
insecticides and, as a result, have not been widely adopted by the farmers (Kumari
et al. 2010). Another potential soil-dwelling biocontrol agents are
entomopathogenic nematodes (EPNs), which can be used as an important tool for
Helicoverpa control (Richter and Fuxa 1990; Cabanillas and Raulston 1996a, b;
Feaster and Steinkraus 1996; Raulston et al. 2001; Hussain et al. 2014).
For any successful IPM programme, one must have complete understanding of
the factors that regulate the pest population. Every effort should be made to take
advantage of weak points in the biology of the pest to devise the control measures.
Therefore, a brief account of Helicoverpa spp. (particularly H. armigera and
H. zea) as well as the potential of EPNs and its limitations is discussed in this
chapter to correlate a better suite of control tactics utilising this bio-agent.
14.2 Biology of Helicoverpa spp.
Morphology and biology of various life stages of Helicoverpa spp. have been
described by various workers (Hardwick 1965; Armes 1989; Matthews 1999,
etc.). Sharma (2001) gave a comprehensive account of the biology of
H. armigera which is typical of the noctuid insect. Life cycle consists of four
stages, viz., egg, larva, pupa and adult. A female lays 500–3,000 eggs singly on its
host plants. Oviposition period lasts for 5–24 days, and the duration of the egg
incubation period varies between 2 and 5 days. The newly emerged larvae eat
empty eggshell and then move around for some distance, with occasional feeding
on the surface before settling down at a preferred site (flower bud or flower in cotton
and pigeon pea, young leaves in chickpea, corn silks and the soft grain or young
whorled leaves of cereals). Older larvae prefer flower buds and young cotton bolls,
legume pods or cereal grain, although leaves are also eaten when plants are small or
only a few fruiting bodies are present. Encounters between older (>third instars)
larvae usually result in cannibalism, often resulting in only one large larva per
flowering bud, boll, pod, whorled leaves or a panicle (Sharma 2001). The average
larval period of 21 days is recorded on cotton flower buds at 21–27 C (Reed 1965),
whereas Singh and Singh (1975) reported 8–12 days on tomato and 18 days on
cotton and maize.
On completion of larval development, the larvae drop or crawl to the ground and
enter the soil for pupation. The depth at which the pupal cell is formed varies
considerably at a depth of 2.5–17.5 cm (Jayaraj 1982) depending on hardness and
wetness of the soil, presence of cracks and crevices and surface litter. Occasionally,
pupation may also take place inside a tunnel in a maize cob (Reed 1965) or on the
soil or leaf surface. The prepupal stage lasts for 1–4 days.
The adults of Helicoverpa spp. are stout-bodied moths. The males are uniformly
pale cream and forewings generally tinged with green, whereas females are darker,
and the forewings are without a green tinge. The longevity of adults depends upon
the availability of food such as nectar, pupal weight, temperature and adult behav-
iour such as flight activity. In the absence of suitable food source, depletion of the
fat bodies is rapid and death occurs in a few days (Armes 1989). In captivity,
longevity varies from 1 to 23 days for males and 5 to 28 days for females. The
importance of Helicoverpa is largely due to its well-developed survival strategies,
diapause and dispersal abilities, which enable it to exploit food sources, separated
by unfavourable times and distance, and it responds largely to local environmental
cues and undertakes short- or long-distance flight in the direction largely governed
by prevailing weather systems (Fitt 1989).
14.3 Helicoverpa spp. Control Methods
Pest management strategies for Helicoverpa spp. require integration of different

control tactics to implement a threshold based on the relationship between popula-
tion density and the economic loss.
14.3.1 ETL and Population Monitoring Through Pheromone
Adult moths are monitored with light and pheromone traps. Field monitoring of
pest populations is necessary to determine whether the threshold has been
exceeded, and control measures should be undertaken. Location-based economic
threshold level (ETL) for H. armigera in cotton is reported by Simwat (1994) as
25 adults/trap/night or 1 larva/10 plants or 20 balls at Bapatla and Coimbatore, 10 %
incidence in reproductive parts at Bhatinda and 2–7 larval unit/10 plants at Surat
(India).
14.3.2 Cultural Control
Early or timely planting of crops can help avoid periods of peak abundance of
H. armigera. The planting times are often decided by the rainfall pattern and
availability of soil moisture. Late planted crops often suffer greater pest damage
because of the build-up of pest populations over the cropping season. Short-season
cultivars are used to minimise bollworm damage in cotton in the USA and plant
growth regulators to shorten the crop maturity (Bradley et al. 1986). Early termi-
nation of flowering and fruiting can also check the population carry-over from one
season to another or reduce the number of generations. Intercropping chickpea with
wheat, mustard or safflower; pigeon pea with cowpea and sorghum; groundnut and
coriander in chickpea; and tomato with radish result in reduced damage by
H. armigera. Intercropping can also be used as a means of encouraging the activity
of natural enemies. Trap crops and diversionary hosts have been widely used in the
past to reduce the damage by H. armigera, but have seldom been successful (Fitt
1989). Sunflower, marigold, sesame and carrot are used as trap crop for H. armigera
control. Ploughing destroys pupae of H. armigera in soil, and flooding with water
affects Helicoverpa pupal survival and moth emergence (Fitt and Cotter 2005).
Irrigation or flooding of cotton fields at the time of pupation reduces pupal survival
and leads to decreased population densities in the following generation or season.
However, chickpea grown under rainfed conditions is not amenable to flooding due
to water scarcity (Sharma et al. 2008).
14.3.3 Host–Plant Resistance
The development of crop cultivars resistant or tolerant to H. armigera has a major

potential for IPM (Lal et al. 1986; Fitt 1989; Sharma et al. 1999), particularly under
subsistence farming in the developing countries. The development of crop varieties
that are resistant or tolerant to H. armigera has received the major attention
particularly for cotton, pigeon pea and chickpea. Many crop species possess some
genetic potential, which can be exploited by breeders to produce varieties that are
less susceptible to H. armigera damage (Sharma 2001). Cultivars with low to
moderate level of resistance to insect pests have been identified in pigeon pea,
chickpea, cowpea, black gram, green gram and field pea (Sharma et al. 2008).
14.3.4 Chemical Control
There is substantial literature on comparative efficacy of different insecticides

against Helicoverpa on cotton, pulses, vegetables and other crops. Information on
chemical control has been reviewed extensively by Zalucki et al. (1986) and
Matthews (1989). The early history of chemical control of corn earworms is given
by Hardwick (1965), while COPR (1983) includes a list of 29 insecticides effective
at the recommended rates. Jackson (2014) describes newer molecules of insecti-
cides for the control of Helicoverpa infesting cotton, soybeans, corn, sorghum and
peanuts. Insecticide resistance management strategies have been developed in
several countries to prevent the development of resistance or to contain it (Sawicki
and Denholm 1987; Whalon et al. 2007).
14.3.5 Biological Control
Information on natural enemies and biological control of Helicoverpa spp. is

reviewed by several workers (King and Coleman 1989; Romeis and Shanower
1996). In India, 75 arthropod parasitoids and 33 predators are reported to occur on
H. armigera (Manjunath et al. 1989). Sharma (2001) published the recent update on
arthropod natural enemies of H. armigera in India including spiders, birds and
microorganisms. In most areas, species of Telenomus, Trichogramma and
Trichogrammatoidea are important egg parasitoids, whereas at least one species
each of Ichneumonidae (e.g. Campoletis chlorideae) and Tachinidae (e.g. Carcelia
illota) is larval parasitoids. The most common predators of Helicoverpa include
species of Chrysopa, Chrysoperla, Nabis, Geocoris, Orius and Polistes and species
belonging to Pentatomidae, Reduviidae, Coccinellidae, Carabidae, Formicidae and
Araneidae (Zalucki et al. 1986; Romeis and Shanower 1996; Sharma 2001).
Although effective in large numbers, high cost of large-scale production precludes
its economic use in biocontrol of H. armigera (King et al. 1986).
There has been considerable interest in the inundative biological control using
Bt and HearSNPV including entomopathogenic fungi and EPNs. H. armigera is
highly susceptible to HearSNPV (Rabindra and Jayaraj 1988) and can be success-
fully controlled if the application coincides with the occurrence of early stages of
the larvae; however, late-stage larvae are more tolerant to the virus, although to
date, these tactics have not provided a viable alternative to insecticides. Granular
formulations of Bt (based on wheat meal and yeast extract as phagostimulant,
protect Bt against environmental degradation and formulation) have been found
to be more effective than Bt sprays (Navon et al. 1997). The entomopathogenic
fungus Nomuraea rileyi at a concentration of 1.0 106 spores/ml resulted in 90–
100 % larval mortality, while Beauveria bassiana at a concentration of
2.7 107 spores/ml resulted in 6 % damage on chickpea compared to 16 % damage
in untreated plots (Saxena and Ahmad 1997).
14.3.6 IPM Strategies
In view of the need to exploit the existing spectra of natural enemies and to reduce
excessive dependence on chemical control, particularly where there is resistance to
insecticides, various IPM programmes have been suggested in which different
control tactics are combined to suppress pest numbers below threshold level
(Sharma 2001, 2005; Deguine et al. 2008; Sharma et al. 2008; Fathipour and
Sedaratian 2013). These vary from judicious use of insecticides, based on economic
thresholds and regular scouting to ascertain pest population levels, to sophisticated
population models to assess the need, optimum timing and selection of insecticides
for sprays. Traps (light, pheromone, suction or wind traps) can be used to monitor
pest populations to develop pest-forecasting models. The model, HEAPS, incorpo-
rates modules based on adult movement, oviposition, development, survival and
host phenology (Dillon and Fitt 1990). Decision support systems for managing
insect pests in cotton in Australia have been updated as SIRATAC and
CottonLOGIC (Hearn and Bange 2002).
Genetic transformation with the Bt genes has been developed; however, the
deployment of transgenic crops for pest management is raising concerns and may
take time to be fully integrated in cultivation. Therefore, host–plant resistance,
cultural practices, biological control with natural enemies and microbial pathogens
remain the backbone of pest management systems favourable to most
agroecosystems (Sharma 2005).
14.4 EPNs as Biological Control Agent of Helicoverpa spp.
EPNs along with their symbiotic bacteria are lethal obligatory parasites of insects
(Gaugler and Kaya 1990; Kaya and Gaugler 1993; Dillman et al. 2012; Poinar and
Grewal 2012; Stock and Goodrich-Blair 2012) (refer to Chap. 13 of this volume for
the detailed account of bacterial symbiosis with EPNs). These beneficial nematodes
can be considered good candidates for IPM and sustainable agriculture due to a
variety of attributes such as rapid insect kill, host-finding ability, recycling persis-
tence in the environment and safety for all vertebrates and nontarget invertebrates
(Lacey and Georgis 2012). However, they exhibit differences in host range, infec-
tivity, environmental tolerance and suitability for commercial production and
formulation. These nematodes have been tested against a large number of insect
pest species with results varying from no effect to excellent control (Begley 1990;
Bedding et al. 1993; Grewal et al. 2005; Georgis et al. 2006; Kaya et al. 2006). The
target insects include those from foliar, soil surface, cryptic and subterranean
habitats (Arthurs et al. 2004; Georgis et al. 2006; Lacey and Georgis 2012).
From the producer’s point of view, marketing EPNs is a success; however, from
the global point of view, the revenues and the market size are limited. According to
CPL Business Consultants report in 2008 and BBC Research report in 2009,
revenue generated from chemical pesticide at the distributor level was US$37,315
million (97.92 %) compared with biopesticides in total (US$814.2 million, 2.08 %)
(Lacey and Georgis 2012). Within the market share occupied by biopesticides, the
revenue from essential/industrial oil, plant extract and others was maximum
(66.2 %) followed by Bt (18.9 %), B. subtilis (7.8 %), Trichoderma spp. (2.8 %)
and Beauveria spp. (2.7 %), whereas EPN’s share was minimum (1.7 %). Factors
such as cost, shelf life, handling, mixing, coverage, new caution signal-based
pesticides, compatibility and profit margins to manufacturers and distributors
have prevented nematode-based biopesticides to gain significant market share
(Lacey and Georgis 2012).
14.4.1 Susceptibility of Helicoverpa spp. at Different Life

Stages
Of the four different life stages of Helicoverpa spp., larvae and pupae are most
susceptible to EPNs (Cabanillas and Raulston 1994; Raulston et al. 2001; Hussain
and Ahmad 2011). A literature search on the infectivity, bioefficacy or susceptibility
of Helicoverpa spp. to EPNs retrieves numerous references; describing all of them
seems unnecessary and even not required; therefore, only some of the relevant works
are discussed to highlight the issue. The pathogenicity of EPNs to Helicoverpa
species has been demonstrated previously (Tanada and Reiner 1962; Samsook and
Sikora 1981; Bong and Sikorowski 1983). Several other authors carried out the
susceptibility studies and reported Steinernema abbasi, S. carpocapsae, S. feltiae,
S. glaseri, S. masoodi (Species inquirenda by Nguyen and Hunt 2007), S. riobrave,
S. siamkayai, Heterorhabditis bacteriophora and H. indica as effective in killing
Helicoverpa larvae under laboratory conditions (Glazer and Navon 1989; Karunakar
et al. 1999; Navon et al. 2002; Jothi and Mehta 2006; Shoeb et al. 2006; Hussain and
Ahmad 2011).
The dose-mortality response indicates that H. armigera is highly susceptible to
infective juveniles (IJs) with respect to different larval stages. Laboratory bioassays
showed 100 % mortality of fourth instar larvae of H. armigera at 72 h post-exposure
of 75 IJs of H. indica (Meerut strain) per larva (Fig. 14.1). In filter paper bioassay,
mean larval mortality of different stages of H. armigera ranged from 43 to 75 %
after 72 h post-exposure of various concentrations ranging from 25 to 150 IJs of
S. masoodi per larva (Fig. 14.2). Third and fourth instar larvae (74 and 75 %
mortality, respectively) were more susceptible than fifth (66 %) and second
(43 %) instars. At the dose of 100 IJs of S. masoodi/larva, 100 % mortality of
fourth instar larvae was recorded at 72 h post-exposure, whereas lowest mortality
(23 %) of second-stage larva was obtained at nematode concentration of 25 IJs
(Hussain and Ahmad 2011). The variation in virulence of nematodes to different
life stages of insect is reported by several workers as well (Glazer and Navon 1989;
Karunakar et al. 1999; Banu et al. 2007; Stock and Goodrich-Blair 2012). Jothi and
24h 48h 72h a a aa aa aa

b ba
100 c
cd cd
cd
d d d
80
Percent mortality e
e
60 ef
f
40 f
g gh
hi
20 i
j j j
0
0 10 25 50 75 100 200 300 400 500
Nematode concentrations (infective juveniles per larva)
Fig. 14.1 The percent mortality of fourth instar larvae of Helicoverpa armigera observed at
24, 48 and 72 h post-exposure of various concentrations of Heterorhabditis indica (Meerut strain)
infective juveniles at 30 1 C and 92 % RH. Bars (means, arcsine transformed values) indicated
with the same letter are not significantly different according to LSD test at P < 0.05. Error
bar ¼ standard error of means (reproduced from Prasad et al. 2012)
Mehta (2006) obtained 83 % mortality of H. armigera (irrespective of larval stages)

at 72 h after inoculation of S. glaseri, and 100 % mortality of third to fifth instar
larvae was obtained at a dose of 40 IJs. Shoeb et al. (2006) recorded 93 % mortality
of fourth instar larvae of H. armigera by S. abbasi and H. bacteriophora at 72 h
post-exposure. One hundred percent mortality of last instar larvae of H. armigera
was achieved with 200 IJs of S. feltiae (All strain) (Glazer and Navon 1989) and by
40 IJs of S. glaseri or S. feltiae (Karunakar et al. 1999). Mortality of 100 % fourth
instar larvae of H. armigera was caused by feeding them on 1,000 IJ/g of
S. carpocapsae (All strain) in the calcium alginate gel for 24 h, whereas mortality
of larvae exposed to 500 IJ/g of gel ranged from 45–55 % at 4 h to 90–95 % at 48 h
(Fig. 14.3).
The developmental stage of the insect plays an important role in susceptibility to
the nematode. There exists a negative relationship between larval age and suscep-
tibility to the nematodes (Samsook and Sikora 1981; Glazer and Navon 1989).
There are many reasons that could explain the differences, including the size,
immune response and host behaviour of the host insect. The portals of entry for
nematodes may be smaller in the younger instars (Jackson and Brooks 1995), and
smaller instars may be less attractive in terms of host cues such as CO2 or
kairomones (Kaya 1985). However, in a grown-up insect larva, nematodes may
get crushed by insect’s mandibles (Gaugler and Molloy 1981), or frequent defeca-
tion may expel nematodes entering through anus (Dowds and Peters 2002). After
invasion, older larvae may also become less susceptible if nematodes fail evasion of
host defences and are not able to overcome the insect’s immune system (Simões
and Rosa 1996).
The susceptibility of heliothinid prepupae and pupae to nematodes is reported by
several authors, for example, H. armigera and H. zea prepupae and/or pupae of
H. indica, S. carpocapsae, S. glaseri, S. riobrave and S. masoodi (Kaya and Hara
2nd instar 3rd instar 4rd instar 5rd instar

100 a
ab ab
abc abc abc abc
cd
Mean % larval mortality
80 cd bc
(Abbott corrected)
de e de e
e e
60 f
f f
40 g
20
h h h h
0
0 25 50 75 100 150
Nematode concentrations (IJs)
Fig. 14.2 The percent mortality of second to fifth instars of Helicoverpa armigera after 72 h
exposure to various concentrations of Steinernema masoodi infective juveniles at 28 C and 92 %
RH. Error bars indicate the standard errors of the means. Bars (means of three replicates where
each replicate comprised of 12 H. armigera larvae) indicated with the same letter are not
significantly different according to Tukey’s HSD test at P < 0.05 (arcsine transformed values)
(reproduced from Hussain and Ahmad 2011)
100
AB A a A
H. armigera B
a
90 S. littoralis
ab
80 b
C C
Larval mortality (%)
70 c
60 c
50
40
30
20
10
0
0 4 8 12 24 32 48
Exposure time (h)
Fig. 14.3 Effect of larval exposure time to nematodes on the mortality of fourth instar larvae of
Helicoverpa armigera and fifth instar Spodoptera littoralis feeding for 24 h on the nematode
S. carpocapsae (All strain) in an alginate gel at 500 IJ/g. Means denoted by the same lower-case
letter for H. armigera and capital letter for S. littoralis larvae are not significantly different
(P < 0.05). Two-way ANOVA followed by a Student–Newman–Keuls (SNK) test (reproduced
from Navon et al. 2002)
1981; Cabanillas and Raulston 1994; Raulston et al. 1992, 2001; Banu et al. 2007;
Hussain and Ahmad 2011). The median lethal concentration of 13 IJs of S. riobrave
per prepupa of H. zea was reported by Cabanillas and Raulston (1994), whereas
Saravanapriya and Subramanian (2007) recorded LC50 values of 104 and 122 IJs of
H. indica and S. glaseri, respectively, for H. armigera pupa. One hundred percent
mortality of H. zea prepupae was achieved with the exposure to 100 IJs of
S. riobrave (Cabanillas and Raulston 1994). However, lesser infectivity (59 %) to
prepupae was obtained by introduction of 1,000 IJs of S. masoodi per earthen pot
containing 500 g soil (Hussain and Ahmad 2011). The variation in percent kill
could be due to the differences in nematode species, host insect species and/or
testing method employed. In cage condition studies, conducted twice, Bell (1995)
reported 57 and 66 % reduction in H. virescens adult emergence from soil under
cotton plants treated with S. riobrave at dose 2.4 105 IJs/m2. In a survey, the
scouting of prepupae and pupae of H. zea over a period of 5 years from fruiting
maize fields in Texas (USA) and northern Tamaulipas (Mexico) showed the natural
parasitism of 34 % by S. riobrave (Raulston et al. 1992). These results strongly
indicate the control potential of these nematodes in managing soil-dwelling stages
of Helicoverpa species.
14.4.2 Field Efficacy of Nematodes for Helicoverpa spp.
Extensive research over the past three decades has demonstrated successes and
failures of EPNs for the control of insect pests of crops, ornamental plants, trees,
lawn and turf including soil insect pests (Georgis et al. 2006; Kaya et al. 2006;
Lacey and Georgis 2012). Nonetheless, research highlights the potential of such
bio-agents against above-ground pests under certain circumstances. Arthurs
et al. (2004) analysed data from published research articles of field trials in which
these nematodes were applied for control of insect pests in above-ground habitats.
The highest efficacy was found for cryptic habitats compared with exposed foliage
habitat.
14.4.2.1 Foliar Application
The status of the foliar application of nematodes has been reviewed to control insect
pests feeding on above-ground parts (Arthurs et al. 2004; Georgis et al. 2006;
Shapiro-Ilan et al. 2006; Lacey and Georgis 2012). Poor to moderate levels of
suppression were achieved when nematodes were applied to foliage to control
H. armigera and H. zea (Bong and Sikorowski 1983; Glazer and Navon 1989;
Richter and Fuxa 1990; Vyas et al. 2003; Prabhuraj et al. 2008; Hussain and Ahmad
2011).
The limited viability of nematodes on chickpea foliage is reported by Ahmad
et al. (2009) and Prabhuraj et al. (2005). When S. masoodi-sprayed chickpea leaves
and pods were offered to H. armigera larvae soon after spraying, 85 % larval
mortality was recorded after 72 h, whereas larval mortality declined gradually from
95 to 75 %, 55 % and 35 % when nematode-treated foliage at 1, 2 and 3 h post-spray
was offered, respectively (Fig. 14.4). No larval mortality was recorded when leaves
and pods (nematode sprayed at 16 and 24 h post-sprays) were fed to larvae. The
a a
% Helicoverpa armigera larval mortality

100
b 24 48 72
bc
80 bcd
bcdef
bcde
defg defgh
60
efghi
fghij
40 ij
20
k k k
0
0 1 2 3 16 and 24
Hours after Steinernema masoodi foliar application
Fig. 14.4 Efficacy of Steinernema masoodi in killing Helicoverpa armigera fed on S. masoodi-
sprayed chickpea foliage at dose 3 109 IJs/ha. Legends (24, 48 and 72) show the hours after
S. masoodi-treated chickpea twigs fed to H. armigera larvae. Bars (n ¼ 6) indicated with the same
letters are not significantly different according to LSD test at P < 0.05. Error bars indicate the
standard errors of the means (reproduced from Hussain and Ahmad 2011)
results indicate that S. masoodi treatments were effective up to initial 3 h only.

Thereafter, nematodes desiccated over the foliage hampering its viability, mobility
and effectiveness in killing the target insect (Hussain and Ahmad 2011). In contrary
to above results, Patel and Vyas (1995) obtained 24.6 % mortality of H. armigera
6 days after S. glaseri application at the rate of 200 IJs/ml on chickpea in pots,
whereas Vyas et al. (2003) recorded 59 and 71 % larval mortality on the 4th and
6th day after application of Heterorhabditis spp. @ 2,000 IJs/pot, respectively, and
reported increased yield of chickpea. Glazer et al. (1992) reported low (22 %)
effectiveness of S. carpocapsae against H. armigera on cotton at a dose of
5,000 IJs/ml in distilled water; however, higher mortality of 85–95 % was observed
when antidesiccants like Biosys 627, natural wax and Folicote were added in spray
suspension.
In field trial conducted at Kanpur (India), significant results were obtained with
respect to percent pod damage and grain yield. The lowest pod damage (12 %) was
recorded by the foliar application of S. carpocapsae (Kanpur isolate) at a dose of
3 109 IJs/ha + antidesiccant + UV retardant, and chickpea yield of 26.85 q/ha was
obtained resulting in 42 % increased yield over the untreated control (Hussain and
Ahmad 2011). Yet in another field trial carried out at Meerut (India) during 2007–
2008 by Hussain and Ahmad (2011), least pod damage (11 %) was achieved by the
application of 5 109 IJs of H. indica (Meerut strain)/ha + glycerine + Teepol, and
the highest yield of 21.5 q/ha was obtained compared with the control plots (14.6 q/
ha; pod damage 29 %). Hussain and Ahmad (2011) did not obtain greater level of
control as reported by Vyas et al. (2002) and Prabhuraj et al. (2008). In pigeon pea
field trial, Vyas et al. (2002) reported reduced larval population of H. armigera by
17 and 28.5 % over the initial population when Heterorhabditis sp. was sprayed
alone (at a dose of 1 109 IJs/ha) and with adjuvants (5 % starch + gum arabic),
respectively. Similarly, by the foliar spray of H. indica RCR isolate at a dose of

3 105 IJs/12 m2 plot (equivalent to 0.25 109 IJs/ha), Prabhuraj et al. (2008)
recorded chickpea var. A-1 pod damage up to 15.4 and 16.2 % during rabi 2003–
2004 and 2004–2005, respectively, and obtained seed yield of 1.48 kg/plot (equiv-
alent to 12.3 q/ha) and 18.1 q/ha, respectively. This level of control is not accept-
able by end users and still a higher level of control is desirable. Overall, these data
show that EPNs are not really suitable for foliar application to control H. armigera
if we calculate cost-benefit ratio. Newer technologies and/or novel molecules are
warranted to improve the viability of EPNs in order to control insect pests feeding
on above-ground habitat.
The use of EPNs to manage insect pests feeding on aerial parts poses a consid-
erable challenge as above-ground conditions are detrimental to nematodes (Arthurs
et al. 2004). Infective juveniles (IJs) get inactivated quickly and are sensitive to
extremes of physical environment particularly rapid desiccation (Womersley
1990), high temperature (Glazer 2002; Grewal et al. 1994), lethal UV radiation
(Gaugler et al. 1992; Nickle and Shapiro 1992) and difficulty in establishing
attraction gradients (Glazer 1992). To prolong nematode survival on the leaf,
several adjuvants have been evaluated (Glazer 1992; Baur et al. 1997; Mason
et al. 1998), but further improvements are needed to increase the feasibility of
nematode foliar application (Baur et al. 1997; Schroer et al. 2005; Lacey and
Georgis 2012). Besides the infective nematodes itself, other additives and adjuncts
are needed to be formulated into the compositions of nematodes such as humec-
tants, phagostimulants, UV protestants, inert fillers and dispersants, etc. to increase
nematode’s viability and infectivity (Raulston et al. 2001).
14.4.2.2 EPNs Application in Epigeal (Soil Surface) Habitat
The young larvae of Helicoverpa feed on tender foliage, but later instars switch
over to feed on harvestable part of the plant. When EPN is applied on foliage to
control these larvae, abiotic factors reduce the viability of nematodes to few hours
only (Begley 1990; Glazer 1992; Prabhuraj et al. 2005; Ahmad et al. 2009). As a
consequence, Helicoverpa larvae escape nematode’s pathogenic attack and con-
tinue damaging fruiting bodies of the crop. The other possibility to control this
dreaded pest is in the upper soil profile, the very own habitat of EPNs, while last
instar larvae crawl to the ground and enter crevices or loose soil for pupation. There
is likelihood that pupating larvae may encounter infective juveniles present in soil
and infect them prior to insect metamorphosis into the next developmental stage
(Hussain et al. 2014).
The potential of two species of EPNs for control of Helicoverpa spp. needs
mention here. S. riobrave was isolated from soil samples taken from corn plots after
harvest at Lower Rio Grande Valley of Texas, USA (Cabanillas et al. 1994),
whereas S. masoodi was isolated from sandy soil of pigeon pea field when temper-
ature was soaring high (40–45 C) at Kanpur, India (Ali et al. 2005). The thermal
tolerance of both the nematodes suggests that they could play a potential role at
Table 14.2 Percent suppression of Helicoverpa armigera adult emergence by Steinernema

masoodi at prepupal stage in soil under laboratory conditions
Nematode concentration (IJs/60 g soil/ Percent suppression of adult emergencea
container) (SE)
Experiment 1
0 0.8 (0) b
500 80.4 (8.8) a
1,000 76.3 (12.9) a
2,000 89.2 (0) a
3,000 89.2 (0) a
5,000 89.2 (0) a
Experiment 2
0 0.6 (0) c
50 29.9 (3.3) bc
100 45.0 (11.8) ab
150 45.0 (5.8) ab
200 56.8 (0) ab
250 53.8 (3.0) ab
300 63.4 (0) a
350 57.1 (6.3) ab
400 50.9 (5.9) ab
450 64.2 (7.4) a
500 60.1 (3.3) ab
a
Within a column, means (Abbott corrected and arcsine transformed values) followed by the same
lower-case letter are not significantly different, ANOVA, Tukey’s HSD test at P < 0.05. Exper-
iments 1 and 2 were analysed separately. Means of three replicates where each replicate comprised
of ten last instar larvae of H. armigera (reproduced from Hussain et al. 2014)
high temperature regimes in the management of pupating H. armigera (Gouge

et al. 1999; Ali et al. 2007). Both the nematode species were evaluated for the
suppression of H. armigera and H. zea at prepupal stage while undergoing pupation
in soil. In filter paper bioassay, exposure of 10, 20, 40, 80 and 100 IJs of S. riobrave
per prepupa resulted in mortalities of 40, 55, 85, 90 and 100 %, respectively
(Cabanillas and Raulston 1994), whereas in laboratory, suppression of 76–89 %
adult emergence was obtained with no significant differences at nematode concen-
trations 500, 1,000, 2,000, 3,000 and 5,000 IJs of S. masoodi/60 g soil/last instar
larva (Hussain et al. 2014). However, at lower concentrations (0–500 IJs/60 g soil/
larva with increment of 50 IJs in the treatments), varied percent suppression was
recorded, highest (64 %) being at 450 IJs/60 g soil/larva and lowest (30 %) at 50 IJs/
60 g soil/larva (Table 14.2).
Cabanillas et al. (2005) describe many factors that play crucial roles in the
successful use of nematode in corn earworm, H. zea control. First, nematode
applications should be matched with the most susceptible stage of the maize
earworm. Cabanillas and Raulston (1995) obtained insect mortalities of 100 and
95 % in maize fields by applying S. riobrave to the soil when 50 % of the larvae
were late instars (still in the maize ears) and when 10 % of the larvae had left the
ears to pupate, respectively (Fig. 14.5). Second, irrigation method and timing, and
nematode concentration should be optimum. In chickpea micro-plot, the suppres-
sion of 70 % adult emergence was obtained at the dose of 600,000 IJs/m2
(Fig. 14.6), whereas S. riobrave, at the most effective nematode concentration of
200,000 nematodes/m2, caused higher insect mortalities when it was applied via
in-furrow irrigation (95 %) than when it was applied after irrigation (84 %) or
before irrigation (56 %) (Fig. 14.7). Third, the nematode species and the application
method should be matched with the target ecosystem. S. riobrave (TX strain), at the
most effective concentration of 200,000 nematodes/m2, caused 95 % maize
earworm prepupae and pupae mortality, while S. carpocapsae (All strain) did not
cause any insect mortality in maize fields (Cabanillas and Raulston 1996b). The
superiority of S. riobrave was attributed to its greater tolerance of warm soil
temperatures (>38 C) compared with S. carpocapsae (Grewal et al. 1994;
Gouge et al. 1999). Cabanillas and Raulston (1996a) found that subsurface nema-
tode incorporation produced higher insect infections than soil surface applications
in the fields that received nematodes before or after irrigation. Subsurface applica-
tion provided greater nematode protection against desiccation and sunlight than soil
surface application (Gaugler 1988). Similarly, Feaster and Steinkraus (1996)
achieved excellent results, by applying S. riobrave to the soil in Arkansas maize
to control maize earworm. Mean mortalities from S. riobrave infections were
79 and 91 % at nematode levels of 3.7 106 and 12.0 106 nematodes/m2 of
soil, respectively. Although similar results were obtained in irrigated and
nonirrigated plots, higher infection occurred in the plots receiving flood irrigation.
Fig. 14.5 Effect of Steinernema riobrave concentration and timing of soil application on para-
sitism of maize earworm Helicoverpa zea prepupae and pupae in maize (reproduced from
Cabanillas and Raulston 1995)
100
% Suppression of adult emergence

80 a
(Abbott corrected)
a
60
b
40
20
c
0
0 32,000 64,000 96,000
Nematode applications (infective juveniles/microplot)
Fig. 14.6 Percent suppression of Helicoverpa armigera adult emergence by Steinernema masoodi
at prepupal stage in chickpea micro-plot (40 cm 40 cm). Infective juveniles were applied to soil
surface, and H. armigera last instar larvae were released in netted micro-plot, and adult emergence
was monitored up to 1 month. Bars (means, Abbott corrected and arcsine transformed values, of
four replicates where each replicate comprised of 20 H. armigera larvae) indicated with the same
letter are not significantly different according to Tukey’s HSD test at P < 0.05. Thin bars are
standard errors of means (reproduced from Hussain et al. 2014)
Fig. 14.7 Effects of irrigation timing and concentration of Steinernema riobrave on mortality of
maize earworm Helicoverpa zea prepupae buried within 6 days after nematode application in soil
in a maize field (reproduced from Cabanillas and Raulston 1996a)
They demonstrated that this nematode has potential as an inundative biocontrol

agent for this pest.
In semiarid tropics, chickpea is grown under rainfed cool-weather crop or
dry-climate crop and on residual moisture (Smithson et al. 1985), but no irrigation
is needed to raise the crop. If desired, only one-time irrigation is given during
flowering and/or fruiting period, which is the most appropriate time of nematode
application as high incidence of H. armigera is expected during this period. Thus,
control option at soil-dwelling stages could play a critical role in the management of
H. armigera at the time when larva is finding a hiding place in cracks, crevices or
loose soil in the field to undergo pupation (Hussain et al. 2014). These results
substantiate a better approach to suppress the population of forthcoming genera-
tions of Helicoverpa spp., thus preventing adult emergence and subsequent migra-
tion and causing damage to cotton, corn, chickpea, pigeon pea or any other host
crops. However, the apprehension of Bergvinson (2005) is that this approach may
have application for commercial crop protection, but the larvae must complete their
development before being controlled, thereby only reducing insect pest pressure for
the next cropping cycle—an approach that is unlikely to be economically feasible
for farmers.
14.4.3 Factors Affecting Success/Failure of Nematodes
In the laboratory, most EPN species infect a variety of insects where host contact is
certain, environmental conditions are optimal and no ecological or behavioural
barriers to infection exist. Foliage-feeding lepidopteran larvae are highly suscepti-
ble to infection in petri dishes, but are seldom impacted in the field, where
nematodes tend to be quickly inactivated by the environmental factors
(i.e. desiccation, radiation, temperature) characteristics of exposed foliage (Kaya
and Gaugler 1993). However, in the field, EPNs attack a significantly narrower host
range than in the laboratory (Georgis et al. 2006). Several factors related to the
nematode’s biology are critical for successful application; foremost is the matching
of appropriate nematode species with the target pest. Proper match of the nematode
to the host includes virulence, host finding and environmental tolerance (Shapiro-
Ilan et al. 2006). If a nematode does not possess a high level of virulence towards
the target pest, there is little hope of success.
Matching the appropriate nematode host-seeking strategy with the pest is also
essential. These nematodes employ different foraging strategies to locate an insect
host, which range from one extreme of sit-and-wait (ambush) to the other of widely
foraging strategy (cruise) (Lewis 2002). Fenton et al. (2001) emphasised that
different species and strains of EPNs are not the same. For instance, S. glaseri
and H. bacteriophora exhibit strong dispersal (cruising) tendencies and are
characterised by high motility and are distributed throughout the soil profile.
Cruisers orient to volatile host cues and switch to a localised search after host
contact and are well adapted to infect deep soil-dwelling sedentary hosts such as
scarab and lepidopteran prepupae and pupae, whereas the sedentary nematodes,
e.g. S. carpocapsae adopt sit-and-wait strategies or ambushers and are characterised
by low motility and a tendency to stay near the soil surface. Ambushers tend not to
respond to volatile and contact host cues unless presented in an appropriate
sequence and are more effective against surface-dwelling pests such as the codling
moth, cutworms and mole crickets near the soil surface (Georgis and Gaugler 1991;
Lewis et al. 1993; Campbell et al. 1995).
Fenton et al. (2001) analyses suggest that pre-emptive application may be the
optimum strategy and applying before pest invasion can result in greater control
than applying afterwards. Simulations showed that, to prevent any damage occur-
ring, it may be preferable to apply nematodes before pest invasion, possibly during
the time window between adult arrival and larval emergence. However, the success
of this approach relies on low levels of nematode mortality. A number of field trials
have shown that survival can be enhanced if plots are heavily irrigated both before
and after application, thereby increasing the period for which pre-emptive control is
viable (Georgis and Gaugler 1991; Downing 1994). The results of trials carried out
by Feaster and Steinkraus (1996) on the use of S. riobrave as a control agent of the
corn earworm H. zea agree with Fenton et al. (2001) analyses; nematodes applied
24 h before pest invasion resulted in greater suppression than a larger dose of
nematodes applied 24 h after pest invasion. Additionally, irrigated plots produced
greater rates of suppression than nonirrigated, through enhanced nematode survival.
14.5 Future Perspective of Helicoverpa spp. Control

with Nematodes
Since the release of first transgenic crops in 1996, the option for the management of
Helicoverpa spp. by EPNs was taken a back seat and given the least priority as it is a
costly business compared with the deployment of Bt crops. The major concern of Bt
cotton has been widespread development of insect resistance to the Bt toxins, but
this has so far proven to be minimal. The future of transgenic crops appears stable
for some time to come (James 2012). Shapiro-IIan et al. (2002) support the current
management tactics through the use of transgenic crop that is far more economical
than nematodes and, unlike nematodes, will provide control of several major cotton
pests simultaneously, including pink bollworm and tobacco budworm. For exam-
ple, the seed cost of transgenic cotton is US$116.54 per ha in the USA (Finger
et al. 2011), whereas nematode costs US$1,895 at the recommended dose of
2.5 109 infective nematodes per ha in a single application.
The bollworm appears to be a prime example of an insect that can be managed
using EPNs, but for practical reasons, nematodes will remain unused in conven-
tional cotton (Shapiro-IIan et al. 2002). That’s the reason why we don’t see
literature after 1996 on large-scale field trials with nematodes for the control of
Helicoverpa spp. in cotton, corn, soybeans, tomato or any other high-value cash
crops wherever Bt transgenic crops are cultivated. However, the interest of using
nematodes in refuge is renewed as to delay the development of resistance in insects.
The experimental and modelling results suggest that these nematodes could slow
the evolution of pest resistance to Bt crops, but only under some conditions
(Gassmann et al. 2008, 2012). In combination with the high dose-refuge strategy,
other strategies such as stacking two or more insecticidal genes in the same plant;
tissue-, time- or signal-dependent expression of transgene; rotation of insecticidal
toxins with different modes of action; and deploying different toxins in different
crops in a production system can be used to avoid or delay the development of
resistance in insects to transgenic plants (Gopala Swamy et al. 2009). For example,
transgenic Arabidopsis thaliana plant expressing the 283-kDa protein, toxin A,
encoded by tcdA gene of EPN’s symbiotic bacteria (Photorhabdus luminescens)
conferred resistance to corn rootworm (Diabrotica undecimpunctata) and had a
strong growth-inhibitory effect on the insect (Liu et al. 2003).
The phenomenon of anhydrobiosis inherited in several groups of plant parasitic
nematodes [such as stem and bulb nematode (Ditylenchus dipsaci), seed gall
nematode (Anguina tritici) and white tip nematode (Aphelenchoides besseyi)
remain viable for several years in quiescent stage (Duncan and Moens 2006; Wright
and Perry 2006)] is always baffling us why these nematodes show true
anhydrobiotic behaviour whereas EPNs do not. EPNs exhibit only quiescent
anhydrobiosis (Womersley 1990). Is there anything that can be done in this
direction of research to bring the trait of anhydrobiosis in EPNs by utilising
molecular and biotechnological means? Grewal et al. (2006) and Perry
et al. (2012) reviewed the research work on the physiology, genetics and molecular
biology of anhydrobiosis in the infective juveniles. If this is really possible, then
this innovation will undoubtedly contribute to the expansion of EPNs as biocontrol
agent of most of the susceptible insect pests encompassing above- as well as below-
ground habitats. The advantages of incorporated trait of anhydrobiosis will keep the
EPNs and their symbiotic bacteria in inactivated form not only in formulation and
prolonged storage but also on foliage after above-ground application and resume
their activity upon getting favourable condition. If this is not possible, then there
must be something else such as novel molecules in nature or may be invented in
near future, which can be admixed to encapsulate these nematodes and safeguard
them from detrimental environmental conditions upon application, but
recommence their activities soon after getting entered actively or passively by a
susceptible host insect.
14.6 Conclusions
The crop production has been severely threatened by increasing difficulties in

controlling Helicoverpa spp. as it has developed high levels of resistance to
commonly used insecticides. Use of bio-intensive IPM strategies can reduce
existing overdependence on insecticides and their negative effects on the environ-
ment. Manipulation of cultural practices, host–plant resistance including the
deployment of transgenic crops and biological control could play a crucial role in
reducing the ravages caused by this pest. Among microbial control agents, EPNs
(Steinernema and Heterorhabditis) have been used to control insect pests. A
number of studies support the effectiveness of these nematodes in controlling

H. armigera in laboratory as well as field conditions. However, the outcome of
foliar applications indicates that nematode alone is not effective on foliage; as a
consequence, Helicoverpa larvae escape nematode’s pathogenic attack and con-
tinue damaging fruiting bodies. However, charging nematode in irrigation water
could substantiate an approach to suppress the forthcoming generations of
Helicoverpa spp., thus preventing adult emergence, subsequent migration and
damage to succeeding host crops.
Acknowledgements We are grateful to anonymous colleagues for reviewing the manuscript and
for providing useful comments and suggestions.
References
Acharjee S, Sarmah BK (2013) Transgenic Bacillus thuringiensis (Bt) chickpea: India’s most
wanted genetically modified (GM) pulse crop. Afr J Biotechnol 12:5709–5713
Ahmad R, Hussain MA, Ali SS, Pervez R (2009) Survival of Steinernema masoodi and
S. carpocapsae (Rhabditida: Steinernematidae) on pigeonpea and chickpea after foliar appli-
cation. Arch Phytopathol Plant Protect 42:112–117
Ali SS, Shaheen A, Pervez R, Hussain MA (2005) Steinernema masoodi sp. n. and S. seemae
sp. n. (Nematoda: Rhabditida: Steinernematidae) from India. Int J Nematol 15:89–99
Ali SS, Pervez R, Hussain MA, Ahmad R (2007) Effect of temperature on survival of Steinernema
seemae, S. masoodi and S. carpocapsae (Rhabditida: Steinernematidae) and their subsequent
infectivity to prepupa of Helicoverpa armigera (Hübner). Arch Phytopathol Plant Protect
40:183–187
Alvi AHK, Sayyed AH, Naeem M, Ali M (2012) Field evolved resistance in Helicoverpa armigera
(Lepidoptera: Noctuidae) to Bacillus thuringiensis Toxin Cry1Ac in Pakistan. PLoS One 7
(10):e47309
Armes NJ (1989) Summary of life-table experiments on Heliothis armigera (Hübner) (Lepidop-
tera: Noctuidae). Natural Resources Institute, Chetham, p 55
Armes NJ, Jadhav DR, DeSouza KR (1996) A survey of insecticide resistance in Helicoverpa
armigera in the Indian subcontinent. Bull Entomol Res 86:499–514
Arthurs S, Heinz KM, Prasifka JR (2004) An analysis of using entomopathogenic nematodes
against above-ground pests. Bull Entomol Res 94:297–306
Banu JG, Jothi BD, Narkhedkar NG (2007) Susceptibility of different stages of cotton bollworm,
Helicoverpa armigera (Lepidoptera: Noctuidae) to entomopathogenic nematodes. Int J
Nematol 17:41–45
Barwale RB, Godwal VR, Usha Z, Zehr B (2004) Prospects for Bt cotton technology in India.
AgBioForum 7:23–26
Baur ME, Kaya HK, Gaugler R, Tabashnik B (1997) Effects of adjuvants on entomopathogenic
nematode persistence and efficacy against Plutella xylostella. Biocontrol Sci Technol
7:513–525
Bedding R, Akhurst R, Kaya HK (eds) (1993) Nematodes and the biological control of insects.
CSRIO, East Melbourne
Begley JW (1990) Efficacy against insects in habitats other than soil. In: Gaugler R, Kaya HK
(eds) Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, pp 215–231
Bell MR (1995) Effects of an entomopathogenic nematode and a nuclear polyhedrosis virus on
emergence of Heliothis virescens (Lepidoptera: Noctuidae). J Entomol Sci 30:243–250
Bergvinson DJ (2005) Heliothis/Helicoverpa problem in the Americas: biology and management.

In: Sharma HC (ed) Heliothis/Helicoverpa management. Oxford and IBH Publishing,
New Delhi, pp 7–37
Bong CFJ, Sikorowski PP (1983) Use of the DD 136 strain of Neoaplectana carpocapsae Weiser
(Rhabditida: Steinernematidae) for control of corn earworm (Lepidoptera: Noctuidae). J Econ
Entomol 76:590–593
Bradley JR, Herzog GA, Roach SA, Stinner RE, Terry LI (1986) Cultural control in southeastern
US cropping systems. In: Johnson SJ, King EG, Bradley JR (eds) Theory and tactics of
Heliothis population management: 1. cultural and biological control, vol 316. Southern Coop-
erative Series Bull, pp 22–27
Cabanillas HE, Raulston JR (1994) Pathogenicity of Steinernema riobravis against corn earworm,
Helicoverpa zea (Boddie). Fundam Appl Nematol 17:219–223
Cabanillas HE, Raulston JR (1995) Impact of Steinernema riobravis (Rhabditida:
Steinernematidae) on the control of Helicoverpa zea (Lepidoptera: Noctuidae) in corn. J
Econ Entomol 88:58–64
Cabanillas HE, Raulston JR (1996a) Effects of furrow irrigation on the distribution and infectivity
of Steinernema riobravis against corn earworm in corn. Fundam Appl Nematol 19:273–281
Cabanillas HE, Raulston JR (1996b) Evaluation of Steinernema riobravis, S. carpocapsae, and
irrigation timing for the control of corn earworm, Helicoverpa zea. J Nematol 28:75–82
Cabanillas HE, Poinar GO Jr, Raulston JR (1994) Steinernema riobravis sp. nov. (Rhabditida:
Steinernematidae) from Texas. Fundam Appl Nematol 17:123–131
Cabanillas HE, Wright RJ, Vyas RV (2005) Cereal, fibre, oilseed and medicinal crop applications.
In: Grewal PS, Ehlers R-U, Shapiro-Ilan DI (eds) Nematodes as biocontrol agents. CABI
Campbell JF, Lewis E, Yoder F, Gaugler R (1995) Entomopathogenic nematode
(Heterorhabditidae and Steinernematidae) seasonal population dynamics and impact on insect
populations in turfgrass. Biol Control 5:598–606
Cook SM, Khan ZR, Pickett JA (2007) The use of push-pull strategies in integrated pest
management. Annu Rev Entomol 52:375–400
COPR (1983) Pest control in tropical tomatoes. COPR, London
Deguine JP, Ferron P, Russell D (2008) Sustainable pest management for cotton production: a
review. Agron Sustain Dev 28:113–137
Dillman AR, Chaston JM, Adams BJ, Ciche TA, Goodrich-Blair H, Stock SP, Sternberg PW
(2012) An entomopathogenic nematode by any other name. PLoS Pathog 8:e1002527
Dillon ML, Fitt GP (1990) HEAPS: a regional model of Heliothis population dynamics. In:
Proceedings of the 5th Australian cotton conference, 8–9 August 1990, Broadbeach, Queens-
land. Australian Cotton Growers’ Research Association, Brisbane, Queensland, Australia, pp
337–344
Dowds BCA, Peters A (2002) Virulence mechanisms. In: Gaugler R (ed) Entomopathogenic
nematology. CABI Publishing, Wallingford, pp 79–98
Downes S, Parker T, Mahon R (2010) Incipient resistance of Helicoverpa punctigera to the
Cry2Ab Bt toxin in Bollgard II® cotton. PLoS One 5:e12567
Downing AS (1994) Effect of irrigation and spray volume on efficacy of entomopathogenic
nematodes (Rhabditida: Heterorhabditidae) against white grubs (Coleoptera: Scarabaeidae). J
Econ Entomol 87:643–646
Duncan LW, Moens M (2006) Migratory endoparasitic nematodes. In: Perry RN, Moens M (eds)
Plant nematology. CAB International, Wallingford, pp 123–152
Fathipour Y, Sedaratian A (2013) Integrated management of Helicoverpa armigera in soybean
cropping systems. In: El-Shemy HA (ed) Soybean – pest resistance. InTech, Rijeka, pp
231–280. http://dx.doi.org/10.5772/54522
Feaster MA, Steinkraus DC (1996) Inundative biological control of Helicoverpa zea (Lepidoptera:
Noctuidae) with the entomopathogenic nematode Steinernema riobravis (Rhabditida:
Steinernematidae). Biol Control 7:38–43
Fenton A, Norman R, Fairbairn JP, Hudson PJ (2001) Evaluating the efficacy of entomopathogenic
nematodes for the biological control of crop pests: a nonequilibrium approach. Am Nat
158:408–425
Finger R, Benni NE, Kaphengst T, Evans C, Herbert S, Lehmann B, Morse S, Stupak N (2011) A
meta analysis on farm-level costs and benefits of GM crops. Sustainability 3:743–762
Fitt GP (1989) The ecology of Heliothis species in relation to agroecosystems. Annu Rev Entomol
34:17–52
Fitt GP, Cotter SC (2005) The Helicoverpa problem in Australia: biology and management. In:
Sharma HC (ed) Heliothis/Helicoverpa management: emerging trends and strategies for future
research. Oxford and IBH Publishing, New Delhi, pp 45–61
Gassmann AJ, Stock SP, Sisterson MS, Carrière Y, Tabashnik BE (2008) Synergism between
entomopathogenic nematodes and Bacillus thuringiensis crops: integrating biological control
and resistance management. J Appl Ecol 45:957–966
Gassmann AJ, Hannon ER, Sisterson MS, Stock SP, Carrière Y, Tabashnik BE (2012) Effects of
entomopathogenic nematodes on evolution of pink bollworm resistance to Bacillus
thuringiensis toxin Cry1Ac. J Econ Entomol 105:994–1005
Gaugler R (1988) Ecological considerations in the biological control of soil-inhabiting insects with
entomopathogenic nematodes. Agric Ecosyst Environ 24:351–360
Gaugler R, Kaya HK (eds) (1990) Entomopathogenic nematodes in biological control. CRC Press,
Boca Raton, p 365
Gaugler R, Molloy D (1981) Instar susceptibility to Simulium vittatum (Diptera: Simuliidae) to the
entomogenous nematode Neoaplectana carpocapsae. J Nematol 13:1–5
Gaugler R, Bednarek A, Campbell JF (1992) Ultraviolet inactivation of heterorhabditid and
steinernematid nematodes. J Invertebr Pathol 59:155–160
Georgis R, Gaugler R (1991) Predictability in biological control using entomopathogenic nema-
todes. J Econ Entomol 84:713–720
Georgis R, Koppenhöfer AM, Lacey LA, Bélair G, Duncan LW, Grewal PS, Samish M, Tan L,
Torr P, van Tol RWHM (2006) Successes and failures in the use of parasitic nematodes for pest
control. Biol Control 38:103–123
Glazer I (1992) Survival and efficacy of Steinernema carpocapsae in an exposed environment.
Glazer I (2002) Survival biology. In: Gaugler R (ed) Entomopathogenic nematology. CABI
Glazer I, Navon A (1989) Activity and persistence of entomoparasitic nematodes tested against
Heliothis armigera (Lepidoptera, Noctuidae). J Econ Entomol 83:1795–1800
Glazer I, Klein M, Novan A, Nakache Y (1992) Comparison of efficacy of entomopathogenic
nematodes combined with antidesiccants applied by canopy sprays against three cotton pests
(Lepidoptera: Noctuidae). J Econ Entomol 85:1636–1641
Gopala Swamy SVS, Prasad NVVSD, Rao NHP (2009) Transgenic Bt crops: a major component
of integrated pest management – an overview. Indian J Crop Sci 4:1–10
Gouge DH, Lee LL, Henneberry TJ (1999) Effect of temperature and lepidopteran host species on
entomopathogenic nematode (Nematoda: Steinernematidae, Heterorhabditidae) infection.
Environ Entomol 28:876–883
Grewal PS, Selvan S, Gaugler R (1994) Thermal adaptation of entomopathogenic nematodes:
niche breadth for infection, establishment, and reproduction. J Thermal Biol 19:245–253
Grewal PS, Ehlers R-U, Shapiro-Ilan DI (eds) (2005) Nematodes as biocontrol agents. CABI
Publishing, Wallingford, p 505
Grewal PS, Bornstein-Forst S, Burnell AM, Glazer I, Jagdale GB (2006) Physiological, genetic,
and molecular mechanisms of chemoreception, thermobiosis, and anhydrobiosis in
entomopathogenic nematodes. Biol Control 38:54–65
Hardwick DF (1965) The corn earworm complex. Memoirs Entomol Soc Canada 40:1–247
Hearn AB, Bange MP (2002) SIRATAC and CottonLOGIC: persevering with DSSs in the
Australian cotton industry. Agric Syst 74:27–56
Hussain MA, Ahmad W (2011) Management of Helicoverpa armigera by entomopathogenic

nematodes. Lambert Academic Publishing GmbH & Co. KG, Saarbrücken, p 168
Hussain MA, Ahmad R, Ahmad W (2014) Evaluation of Steinernema masoodi (Rhabditida:
Steinernematidae) against soil-dwelling life stage of Helicoverpa armigera (Lepidoptera:
Noctuidae) in laboratory and microplot study. Can J Plant Protect 2:4–8
Jackson GB (2014) Insect control guide for agronomic crops. Mississippi State University
Extension Service, Publication No. 2471, p 104
Jackson JJ, Brooks MA (1995) Parasitism of western corn rootworm larvae and pupae by
Steinernema carpocapsae. J Nematol 27:15–20
Jallow MFA, Cunningham JP, Zalucki MP (2004) Intra-specific variation for host plant use in
Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae): implications for management. Crop
Prot 23:955–964
James C (2012) Global status of commercialized biotech/GM crops: 2012. ISAAA Brief No. 44.
ISAAA, Ithaca
Jayaraj S (1982) Biological and ecological studies of Heliothis. In: Reed W, Kumble V (eds)
Proceedings of the international workshop on Heliothis management, 15–20 November 1981.
ICRISAT, Patancheru, pp 17–28
Jothi BD, Mehta UK (2006) Pathogenicity of three species of EPN against cotton bollworm
Helicoverpa armigera Hüb. Entomon 31:259–266
Karunakar G, Easwaramoorthy S, David H (1999) Susceptibility of nine lepidopteran insects to
Steinernema glaseri, S. feltiae and Heterorhabditis indicus infection. Int J Nematol 9:68–71
Kaya HK (1985) Susceptibility of early larval stages of Pseudaletia unipuncta and Spodoptera
exigua (Lepidoptera: Noctuidae) to the entomogenous nematode Steinernema feltiae
(Rhabditida: Steinernematidae). J Invertebr Pathol 46:58–62
Kaya HK, Gaugler R (1993) Entomopathogenic nematodes. Annu Rev Entomol 38:181–206
Kaya HK, Hara AH (1981) Susceptibility of various species of lepidopterous pupae to the
entomogenous nematode Neoaplectana carpocapsae. J Nematol 13:291–294
Kaya HK, Aguillera MM, Alumai A, Choo HY, de la Torre M, Fodor A, Ganguly S, Hazir S,
Lakatos T, Pye A, Wilson M, Yamanaka S, Yang H, Ehlers R-U (2006) Status of
entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions
of the world. Biol Control 38:134–155
King EG, Coleman RJ (1989) Potential for biological control of Heliothis species. Annu Rev
Entomol 34:53–75
King EG, Baumhover A, Bouse LF, Greany P, Hartstack AW, Hooper KR, Knipling EF, Morrison
RK, Nettles WC, Powell JE (1986) Augmentation of entomophagous arthropods. In: Johnson
SJ, King EG, Bradley JR (eds) Theory and tactics of Heliothis. Population management.
1. Cultural and biological control, vol 316. Southern Cooperative Series Bull, pp 116–131
Kranthi KR, Jadhav DR, Kranthi S, Wanjari RR, Ali SS, Russel DA (2002) Insecticide resistance
in five major insect pests of cotton in India. Crop Prot 21:449–460
Kumari A, Reddy DJ, Sharma HC (2010) Stability of resistance to pod borer, Helicoverpa
armigera in pigeonpea. Indian J Plant Protect 38:6–12
Lal SS, Yadava CP, Sachan JN (1986) Strategies for the development of an integrated approach to
control grain pod borer, Heliothis armigera infesting chickpea. Pesticides 20:39–51
Lewis EE (2002) Behavioural ecology. In: Gaugler R (ed) Entomopathogenic nematology. CABI
Lewis EE, Gaugler R, Harrison R (1993) Response of cruiser and ambusher entomopathogenic
nematodes (Steinernematidae) to host volatile cues. Can J Zool 71:765–769
Liao C, Heckel DG, Akhursta R (2002) Toxicity of Bacillus thuringiensis insecticidal proteins for
Helicoverpa armigera and Helicoverpa punctigera (Lepidoptera: Noctuidae), major pests of
cotton. J Invertebr Pathol 80:55–63
Liu D, Burton S, Glancy T, Li Z-S, Hampton R, Meade T, Merlo DJ (2003) Insect resistance
conferred by 283-kDa Photorhabdus luminescens protein TcdA in Arabidopsis thaliana. Nat
Manjunath TM, Bhatnagar VS, Pawar CS, Sithanantham S (1989) Economic importance of
Heliothis spp. in India and an assessment of their natural enemies and host plants. In: King
EG, Jackson RD (eds) Proceedings of the workshop on biological control of Heliothis:
increasing the effectiveness of natural enemies, 15–20 November 1985. Far Eastern Regional
Research Office, USDA, New Delhi, pp 197–228
Mason JM, Matthews GA, Wright DJ (1998) Screening and selection of adjuvants for the spray
application of entomopathogenic nematodes against a foliar pest. Crop Prot 17:463–470
Matthews GA (1989) Cotton insect pests and their management. Longmans, Harlow, p 199
Matthews M (1999) Heliothine moths of Australia: a guide to pest bollworms and related noctuid
groups, vol 7, Monograph on Australian Lepidoptera. CSIRO Publishing, Callingford, p 320
Mendelsohn M, Kough J, Vaituzis Z, Matthews K (2003) Are Bt crops safe? Nat Biotechnol
21:1003–1009
Mihm JA (ed) (1997) Insect resistant maize: recent advances and utilization. In: Proceedings of an
international symposium held at the International Maize and Wheat Improvement Center
(CIMMYT), 27 November–3 December, 1994. CIMMYT, Mexico, p 304
Naseri B, Fathipour Y, Moharramipour S, Hosseininaveh V, Gatehouse AM (2010) Digestive
proteolytic and amylolytic activities of Helicoverpa armigera in response to feeding on
different soybean cultivars. Pest Manag Sci 66:1316–1323
Navon A (2000) Bacillus thuringiensis insecticides in crop protection – reality and prospects. Crop
Prot 19:669–676
Navon A, Keren S, Levski S, Grinstein A, Riven Y (1997) Granular feeding baits based on Bacillus
thuringiensis products for the control of lepidopterous pests. Phytoparasitica 25:101–110
Navon A, Nagalakshmi VK, Levski S, Salame L, Glazer I (2002) Effectiveness of
entomopathogenic nematodes in an alginate gel formulation against lepidopterous pests.
Nguyen KB, Hunt DJ (eds) (2007) Entomopathogenic nematodes: systematics, phylogeny and
bacterial symbionts, vol V. Brill, Leiden-Boston, p 816
Nickle WR, Shapiro M (1992) Use of a stilbene brightener, Tinopal LPW, as a radiation protectant
for Steinernema carpocapsae. J Nematol 24:371–373
Patel MC, Vyas RV (1995) Efficacy of Steinernema glaseri against Helicoverpa armigera on
chickpea in pots. Int Chickpea Pigeonpea Newsl 2:39–40
Perry RN, Ehlers R-U, Glazer I (2012) A realistic appraisal of methods to enhance desiccation
tolerance of entomopathogenic nematodes. J Nematol 44:185–190
Poinar GO Jr, Grewal PS (2012) History of entomopathogenic nematology. J Nematol 44:153–161
Prabhuraj A, Girish KS, Shivaleela (2005) Persistence of Heterorhabditis indica on chickpea
foliage. Indian J Nematol 35:24–27
Prabhuraj A, Girish KS, Shivaleela PBV (2008) Integration of Heterorhabditis indica with other
biorationals for managing chickpea pod borer, Helicoverpa armigera (Hüb.). J Biol Control
22:433–448
Prasad CS, Hussain MA, Pal R, Prasad M (2012) Virulence of nematode Heterorhabditis indica
(Meerut strain) against lepidopteran and coleopteran pests. Vegetos 25:343–351
Rabindra RJ, Jayaraj S (1988) Efficacy of NPV with adjuvants as high volume and ultra low
volume application against Heliothis armigera (Hbn.) on chickpea. Trop Pest Manag
34:441–444
Raulston JR, Pair SD, Loera J, Cabanillas HE (1992) Prepupal and pupal parasitism of
Helicoverpa zea and Spodoptera frugiperda (Lepidoptera: Noctuidae) by Steinernema sp. in
cornfields in the Lower Rio Grande Valley. J Econ Entomol 85:1666–1670
Raulston JR, Pair SD, Enrique C (2001) Steinernema sp. nematode for suppression of Helicoverpa
zea and Spodoptera frugiperda. US Patent US 6184434 B1
Reddy GVP, Manjunatha M (2000) Laboratory and field studies on the integrated pest manage-
ment of Helicoverpa armigera (Hübner) in cotton, based on pheromone trap catch threshold
level. J Appl Entomol 124:213–221
Reed W (1965) Heliothis armigera (Hub.) (Noctuidae) in Western Tanganyika. Biology with
special reference to pupal stage. Bull Entomol Res 56:117–125
Reed W, Pawar CS (1982) Heliothis: a global problem. In: Reed W, Kumble V (eds) Proceedings
of the international workshop on Heliothis management, 15–20 November 1981. International
Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Center, Patancheru, pp 9–14
Richter AR, Fuxa JR (1990) Effect of Steinernema feltiae on Spodoptera frugiperda and Heliothis
zea (Lepidoptera: Noctuidae) in corn. J Econ Entomol 83:1286–1291
Romeis J, Shanower TG (1996) Arthropod natural enemies of Helicoverpa armigera (Hübner)
(Lepidoptera: Noctuidae) in India. Biocontrol Sci Technol 6:481–508
Sachan JN, Lal SS (1997) Integrated pest management of pod borer complex of chickpea and
pigeonpea in India. In: Asthana AN, Ali M (eds) Recent advances in pulses research. Indian
Society of Pulses Research and Development, Indian Institute of Pulses Research, Kanpur, pp
349–376
Samsook V, Sikora RA (1981) Influence of parasitic density, host developmental stage and leaf-
wetness duration on Neoaplectana carpocapsae parasitism of Heliothis virescens. Meded Fac
Landbouww Gent 46:685–693
Sanahuja G, Banakar R, Twyman RM, Capell T, Christou P (2011) Bacillus thuringiensis: a
century of research, development and commercial applications. Plant Biotechnol J 9:283–300
Saravanapriya B, Subramanian S (2007) Pathogenicity of EPN to certain foliar insect pests. Ann
Plant Prot Sci 15:219–222
Sawicki RM, Denholm I (1987) Management of resistance to pesticides in cotton. Trop Pest
Manag 33:262–272
Saxena H, Ahmad R (1997) Field evaluation of Beauveria bassiana (Balsamo) Vuillemin against
Helicoverpa armigera (Hübner) infecting chickpea. J Biol Control 11:93–96
Schroer S, Ziermann D, Ehlers R-U (2005) Mode of action of a surfactant-polymer formulation to
support performance of the entomopathogenic nematode Steinernema carpocapsae for control
of diamondback moth larvae (Plutella xylostella). Biocontrol Sci Technol 15:601–613
Shanower TG, Kelley TG, Cowgill SE (1998) Development of effective and environmentally
sound strategies to control Helicoverpa armigera in pigeonpea and chickpea production
systems. In: Saini RK (ed) Proceeding of the 3rd international conference on tropical ento-
mology. International Center for Insect Physiology and Ecology Science Press, Nairobi, pp
239–260
Shapiro-IIan DI, Gouge DH, Koppenhöfer AM (2002) Factors affecting commercial success: case
studies in cotton, turf and citrus. In: Gaugler R (ed) Entomopathogenic nematology. CABI
Shapiro-Ilan DI, Gouge DH, Piggott SJ, Fife JP (2006) Application technology and environmental
considerations for use of entomopathogenic nematodes in biological control. Biol Control
38:124–133
Sharma HC (2001) Cotton bollworm/legume pod borer, Helicoverpa armigera (Hübner)
(Noctuidae: Lepidoptera): biology and management. Crop Protection Compendium, CABI,
Wallingford, p 72
Sharma HC (ed) (2005) Heliothis/Helicoverpa management. Oxford and IBH Publishing,
New Delhi, p 469
Sharma HC (2007) Host plant resistance to insects: modern approaches and limitations. Indian J
Plant Prot 35:170–184
Sharma HC, Singh BU, Hariprasad KV, Bramel-Cox PJ (1999) Host-plant resistance to insects in
integrated pest management for a safer environment. Proc Acad Environ Biol 8:113–136
Sharma HC, Ahmad R, Ujagir R, Yadav RP, Singh R, Ridsdill-Smith TJ (2005) Host plant
resistance to cotton bollworm/legume pod borer, Heliothis/Helicoverpa. In: Sharma HC
(ed) Heliothis/Helicoverpa management: emerging trends and strategies for future research.
Oxford and IBH Publishers, New Delhi, pp 167–208
Sharma HC, Clement SL, Ridsdill-Smith TJ, Ranga Rao GV, El Bouhssini M, Ujagir R, Srivastava
CP, Miles M (2008) Insect pest management in food legumes: the future strategies. In:
Kharkwal MC (ed) Food legumes for nutritional security and sustainable agriculture. Pro-
ceedings of the IVth international food legumes research conference, vol 1. Indian Society of
Genetics and Plant Breeding, New Delhi, pp 522–544
Shoeb MA, Atalla FA, Matar AM (2006) Pathogenicity of the entomopathogenic nematodes,
Steinernema abbasi and Heterorhabditis bacteriophora to certain economic insect pests. Egypt
J Biol Pest Control 16:99–102
Simões N, Rosa JS (1996) Pathogenicity and host specificity of entomopathogenic nematodes.
Simwat GS (1994) Modern concepts in insect pest management in cotton. In: Dhaliwal GS, Arora
R (eds) Trends in agricultural insect pest management. Commonwealth Publishers, New Delhi,
pp 186–237
Singh H, Singh G (1975) Biological studies of Heliothis armigera in Punjab. Indian J Entomol
37:154–164
Smithson JB, Thompson JA, Summerfield RJ (1985) Chickpea (Cicer arietinum L.). In:
Summerfield RJ, Roberts EH (eds) Grain legume crops. Collins, London, pp 312–390
Soleimannejad S, Fathipour Y, Moharramipour S, Zalucki MP (2010) Evaluation of potential
resistance in seeds of different soybean cultivars to Helicoverpa armigera (Lepidoptera:
Noctuidae) using demographic parameters and nutritional indices. J Econ Entomol
103:1420–1430
Stock SP, Goodrich-Blair H (2012) Nematode parasites, pathogens and associates of insects and
invertebrates of economic importance. In: Lacey LA (ed) Manual of techniques in invertebrate
pathology, 2nd edn. Academic, San Diego, pp 373–426
Tanada Y, Reiner C (1962) The use of pathogens in the control of the corn earworm, Heliothis zea
(Boddie). J Insect Pathol 4:139–154
Tay WT, Soria MF, Walsh T, Thomazoni D, Silvie P, Behere GT, Craig Anderson C, Downes S
(2013) A brave new world for an old world pest: Helicoverpa armigera (Lepidoptera:
Noctuidae) in Brazil. PLoS One 8:e80134
van Emden HF, Ball SL, Rao MT (1988) Pest and disease problems in pea, lentil, faba bean, and
chickpea. In: Summerfield RJ (ed) World crops: cool season food legumes. Kluwer Academic
Publishers, Dordrecht, pp 519–534
Vyas RV, Patel NB, Patel P, Patel DJ (2002) Efficacy of entomopathogenic nematode against
Helicoverpa armigera on pigeonpea. Int Chickpea Pigeonpea Newsl 9:43–44
Vyas RV, Patel NB, Yadav P, Ghelani YH, Patel DJ (2003) Performance of entomopathogenic
nematodes for management of gram pod borer, Helicoverpa armigera. Ann Plant Prot Sci
11:107–109
Whalon ME, Mota-Sanchez D, Hollingsworth RM, Duynslayer L (2007) IRAC arthropod pesti-
cide resistance database. Michigan State University, East Lansing, MI. http://www.
pesticideresistance.org/
Womersley CZ (1990) Dehydration survival and anhydrobiotic potential. In: Gaugler R, Kaya HK
(eds) Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, pp 117–137
Wright DJ, Perry RN (2006) Reproduction, physiology and biochemistry. In: Perry RN, Moens M
(eds) Plant nematology. CAB International, Wallingford, pp 187–209
Zalucki MP, Dalglish G, Firempong S, Twine P (1986) The biology and ecology of Heliothis
armigera (Hübner) and H. punctigera Wallengren (Lepidoptera: Noctuidae) in Australia: what
do we know? Aust J Ecol 34:779–814
Zhang H, Yin W, Zhao J, Jin L, Yang Y, Wu S, Tabashnik BE, Wu Y (2011) Early warning of
cotton bollworm resistance associated with intensive planting of Bt cotton in China. PLoS One
6(8):e22874
Chapter 15
Sustainability of Entomopathogenic
Nematodes Against Crop Pests
S. Sivaramakrishnan and M. Razia
15.1 Introduction
Entomopathogenic nematodes (EPNs) are represented as new arsenal for manage-

ment of crop pests above and below ground and are growing steadily at the expense
of conventional pesticides in many countries. Use of biopesticides is expanding,
drawing on developments in biotechnology. In general, EPNs are beneficial to plant
community by regulating the root-invading pests. Few nematode species,
tetradomatids and mermithids, have been used for minor pest control, but they
are host specific, poorly suited to exploit their pest control properties, and difficult
to mass produce. Later in the 1970s, there had been tremendous research and
commercial interest in insect-parasitic nematodes. This interest was sparked due
to the lack of adequate tools to control soil-inhabiting insect pests in an effective,
environmentally acceptable manner and the possibility to produce these nematodes
monoxenically in vitro which was followed by a scale-up of commercial production
levels. More than 100 laboratories and around 70 countries are working on EPNs.
EPNs are small roundworms, about 1 mm long, soft bodied, and non-segmented.
They are obligate or sometimes facultative parasites of insects. Recognition of new
strains and novel species has increased rapidly with the development and improve-
ment of isolation and characterization techniques (Kanga et al. 2012). As of early
2012, 78 valid EPN species have been assigned to Steinernematidae and 15
Heterorhabditidae.
S. Sivaramakrishnan (*)
Department of Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu,
India
M. Razia
Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal 624102,
Tamil Nadu, India

DOI 10.1007/978-3-319-14499-3_15
316 S. Sivaramakrishnan and M. Razia
15.2 Life Cycle of EPNs
In general, the life cycle of nematode includes the egg, four larval stages and the
adult. Nematodes are infective to insects only in their third juvenile stage and size
range from 0.4 to 1.5 mm in length and observed under microscope; they do not
require food and can survive without host in the soil. The infective juveniles
actively locate attack and infect target pests. They enter the host through openings
on the insect body such as mouth and anus. Once inside the insect host, nematodes
release bacteria from their gut. The bacteria multiply and release insecticidal toxins,
which kills the insect within 24–48 h. These bacteria break down the host tissues
which are then taken up by the nematodes as a food. After the host dies, the
nematodes feed on insect tissues and bacteria and reproduced within host. EPN
come out only third stage of juveniles to seek out new hosts. Development of
nematode under optimal conditions (soil temperatures 77–82 F) takes approximately
3–7 days for one life cycle inside a host from egg to egg.
15.2.1 Steinernematids
In the nematode Steinernema, mode of reproduction is amphimictic. The third stage

of IJs matures to become either a male or a female. The life cycle of Steinernema
consists of an egg stage, four juvenile stages, and an adult stage (male and female).
The cycle proceeds from IJs (third-stage infective juveniles) to IJs. Inside the host
the third-stage nematodes, the IJs, develop to adult (males and females) of the first
generation if the nutrients are sufficient. Adult female hatches the eggs and the
juveniles develop to become adult males or females of the second generation. The
completion of life cycle takes 8–10 days. Another route of reproduction exists when
the nutrient supply is insufficient inside the host due to overcrowding of nematodes.
In this case, the IJs develop into adults (males and females) of the first generation,
and eggs produced by the females develop directly into IJs. This cycle completes in
6–7 days. S. scapterisci are less tolerant to lower temperatures and more tolerant to
higher temperatures when compared with other species of the same genus. Sex ratio
in nematodes is influenced by temperature. At 15 and 24 C, females constitute
54 and 60 % of the population, respectively, but at 30 C females constitute 47 % of
the population (Nguyen and Smart 1990).
15.2.2 Heterorhabditids
During the life cycle of Heterorhabditis, the IJs mature to give first-generation
hermaphrodite females, and the females give rise to a second generation of
amphimictic females and IJs. The males and females of Heterorhabditis are
15 Sustainability of Entomopathogenic Nematodes Against Crop Pests 317
environmentally determined. Under in vivo conditions, three adult generations

were produced. Heterorhabditis hermaphrodites lay 1,000 eggs which develop
into second-generation males and females, and the first-generation hermaphrodites
hold about 500 eggs which develop into IJs. Second-generation females lay 6–10
eggs which develop into another generation of adults, and the retained 30 eggs
within the nematode body develop into IJs. Both first and second generations
produced IJs through endotokia matricida. The third-generation females do not
oviposit and their eggs develop into IJs. These nonfeeding IJs come out from insect
cadaver and present in the soil (Wang and Bedding 1996).
15.2.3 Symbiotic Bacteria
Xenorhabdus (Thomas and Poinar 1979) and Photorhabdus (Boemare et al. 1993),
members of the family Enterobacteriaceae, engage in a mutualistic association
with Steinernema and Heterorhabditis spp., respectively. In Steinernema spp. the
bacterial symbionts are carried monoxenically in a special vesicle in the intestine of
IJs, whereas in Heterorhabditis spp. they are present in the entire intestine and
pharynx of IJs. Nematodes provide protection and transport facility to their bacte-
rial symbionts. The bacteria are carried by IJs as a vector and infect the insect host
by penetrating through natural openings such as the mouth, anus, or spiracles. These
bacteria are released from the nematode into the insect’s hemocoel which kill the
host within 48 h. The nematodes consequently reproduce for several generations in
the hemocoel feeding on both the bacteria and the nutrients derived from insect
sources. With the depletion of nutrient supplies, the nematodes develop into IJ stage
acquiring its bacterial partner from the hemocoel before emerging from the insect
cadaver into the soil in order to search for new insect hosts (Poinar 1990; Endo and
Nickle 1991).
Symbiotic bacteria tend to produce two colony forms, a primary form and a
secondary form. The unstable primary form is preferentially taken up by the IJs and
is often converted into the secondary form when cultured in vitro. The mechanism
by which these variants arise is still unidentified. Several genes coding for different
characteristics are switched on and off spontaneously at the same time (Gerritsen
et al. 1992).
This complex life cycle that involves mutualistic as well as pathogenic interac-
tions makes Photorhabdus and Xenorhabdus spp. ideal models to study symbiosis
and pathogenesis. During the life cycle, the bacteria not only have to kill the insect
host using several protein toxins but also have to defeat several other microbes that
are direct food competitors. Saprophytic microbes from the soil as well as bacteria
adhering to the insect gut or cuticle of the nematode represent other potential
sources of competitors that can grow within the insect cadaver.
15.3 EPNs as Biocontrol Agents
EPNs occur naturally in soil environments and locate their host in response to
carbon dioxide, vibrations, and volatile compounds released from insect-infected
plant roots (Lewis et al. 2006). EPNs are robust components of integrated pest
management because they are nontoxic to humans and are relatively specific to
their target pests.
15.3.1 Distribution of EPNs
EPNs have global distribution and numerous surveys have documented their occur-
rence in Australia, Europe, America, South Africa, Sri Lanka, Malaysia, India,
China, and Japan. EPNs are more frequently detected in reduced tillage regimes
(Hummel et al. 2002). In addition, many countries are concerned about the intro-
duction of exotic EPNs, since they might have a harmful impact on nontarget
organisms. Surveys have been conducted in many parts of the world providing a
clue of which species are indigenous for a given area (Kaya et al. 2006).
EPNs applied for insect control are dependent on the motility and persistence of
the applied IJs. Active IJ dispersal is rather limited with up to 90 cm in 30 days in
both horizontal and vertical dispersals to seek suitable host. On the other hand,
inactive nematode dispersal by water, wind, infected hosts, human activity, etc. can
cover much greater distances and increases the chance of prevalent distribution.
Many species and strains of EPNs illustrate a variety of adaptations to extremes in
soil and plant environments. Developmental dormancy and diapause are important
for seasonal survival and long-term longevity. EPNs undergo temporary quiescence
in response to environmental stress and enter into anhydrobiosis or other extreme
states which allow long-term survival in strangely stressful environments. EPNs
infect over 200 insect hosts (Shapiro-Ilan et al. 2002). In agroecosystems, habitat
complexity can be created through the planting of diverse carpet of vegetation
within or adjacent to crop areas that remain undisturbed during the field season.
Abiotic and biotic factors that influence the persistence, infectivity, and motility
of individual IJs also influence nematode recycling. Some of these factors may even
be more crucial for recycling than for persistence and infection.
15.3.2 Behavior of EPNs
Based on the search strategies used, EPNs could be ambushers or cruisers.

Ambushers such as S. carpocapsae have an energy-conserving approach and sit
and wait to attack mobile insects like codling moth, cutworms, and mole crickets in
the upper soil. Cruisers like S. glaseri and H. bacteriophora are highly active and
generally subterranean, moving significant distances using volatile cues to find their
host which are less mobile such as white grubs in the underground soil. A few
nematode species such as S. feltiae and S. riobrave use an intermediate foraging
strategy (combination of ambusher and cruiser type) to find their hosts such as
prepupal insects, fungus gnats, or weevil larvae.
15.3.3 Factors Influencing EPNs
Steinernematidae and Heterorhabditidae are obligate pathogens in nature. They

have been recovered from soils throughout the world and their distribution may be
limited by the availability of susceptible hosts. Habitat density may affect soil biota
positively or negatively due to plant type and diversity (John et al. 2006). The
environmental factors control EPN’s potential in terms of infectivity, motility, and
distribution in the soil environment, the most common being temperature, moisture,
aeration, soil type (texture), and biotic factors (soil fauna) (Shapiro-Ilan et al. 2012).
15.3.3.1 Soil and Moisture
Soil physical properties, such as those characteristic of sandy soils (porous and
aerated), facilitate nematodes when compared to denser soils, such as clay. EPN
species were recovered from rainforest habitats with sandy or sandy loam soil types
and a pH that ranged from slightly acidic to slightly alkaline (Uribe-Lorio
et al. 2005).
Moisture is the most important factor for nematode performance. In soil, IJs
move through the water film that coats the interstitial spaces. In dry soil, the film
becomes too thin and in saturated soil, the interspaces are completely filled with
water; in both cases the nematode movement is restricted. IJs can survive
low-moisture conditions by lowering their rate of metabolism. Gradual water
removal from the IJs gives them time to adapt to the desiccating conditions
(Koppenhfer et al. 1995).
15.3.3.2 Temperature
Temperature is one of the key factors affecting the infectivity of nematodes;

however, the effect of temperature on nematode survival and infectivity varies
with nematode species and strains and their habitats. EPN populations indicate the
temperature and moisture changes due to seasonal variations (Puza and Mracek
2007). Heterorhabditis species are able to cause infection at temperature as low as
5–7 C. Heat tolerance of the IJs under field conditions is one of the important
factors restricting their application on exposed surfaces like foliage and warm
ecosystems. The species S. feltiae can be infective from 2 to 30 C, whereas
some Heterorhabditis can infect host insects from 7 to 35 C, and S. carpocapsae is

nearly inactive at 10 C. More information is required on the relationship between
abiotic factors and nematodes’ persistence and infectivity. A lack of genetic
diversity in the lab-reared populations might be overcome by surviving natural
populations for desired traits. Native isolates have the ability to tolerate the extreme
temperature.
15.3.3.3 pH
Soil pH in most agroecosystems, having a range of 4–8, is not likely to have any
significant effect on EPNs, but a pH of 10 or higher is likely to be detrimental (Kung
et al. 1990). Heterorhabditis is well distributed in coastal sandy soils and is capable
of tolerating a wide range of pH and salinity. The relationship between nematode
populations and soil pH suggests that EPNs may not be as effective on pests in
acidic soils as they are in soils of near-neutral pH. Recommendation on addition of
lime to acidic soils may raise the pH, thus enhancing the effectiveness of nematodes
in farms.
15.3.3.4 Ultra Violet
Emissions produced by UV source corresponding to the specific wavelengths in

solar radiation (290–320 nm) were responsible for inactivation of nematodes.
Consequently, even with adequate moisture, 60 min of direct exposure to sunlight
turned most IJs of Steinernema uninfective. The virulence of nematodes was
unaffected because of trehalose accumulation which may protect the biological
and physiological functions of EPNs against UV stress (Gaugler et al. 1992).
Heterorhabditis might retain significant pathogenicity up to 30 min of exposure
to UV stress (Gaugler and Boush 1978). EPN appears to be the most vulnerable of
all biological insecticides to UV irradiation and also to the influence of sunlight on
persistence and virulence of IJs in exposed habitats (Grewal et al. 2002).
When exposed to natural sunlight, UVA and UVB were responsible for total
inactivation of IJs. In order to protect EPNs from UV irradiation, nematodes can be
encapsulated using starch or other flours.
15.3.3.5 Insect Host
Biotic factors affect nematode survival. Among the natural enemies of nematodes,
nematophagous fungi play a major role, for example, Hirsutella rhossiliensis.
Further natural enemies of infective juveniles include collembolans, mites, tardi-
grades, and predatory nematodes, but their impact under field conditions is not well
understood. Nematodes are unable to attack some insects due to the insect’s
morphology, i.e., structure of insect mouth, for example, IJs are blocked by oral
filters of wireworms, the presence of sucking or piercing or chewing mouthparts,

the anus constricted by muscles, or the spiracles covered with septa or sieve plates
as in scarab grubs (Eidt and Thurston 1995).
15.3.3.6 Tillage
EPN populations vary with respect to cropping and tillage practices in an

agroecosystem. No-till and conservation tillage practices potentially conserve and
thereby enhance native nematodes (Millar and Barbercheck 2002). The nematode
survival and pathogenicity is affected by tillage, EPN population can be preserved
in agroecosystems by recommending reduced tillage practices.
15.3.4 Pathogenicity: Mode of Action of EPN-Associated

Symbiotic Bacteria
Gupta (2002) stated that in insect cellular and humoral immune responses, hemo-
cytes are involved in phagocytosis of microbes and microaggregation and encap-
sulation of large pathogens. Antimicrobial peptides, such as cecropins and
lysozyme, hemolymph clotting system, and activation of the prophenoloxidase
cascade leading to melanization are among the other host responses to the attack
of organisms like bacteria, fungi, or nematodes.
EPN–bacterium complexes are able to kill a wide range of insect species by
overcoming and evading the defense mechanisms of insect host that involves
interactions with the humoral and cellular factors as reported under laboratory
conditions (Gupta 1991). Exo- and endotoxins are produced by EPN-symbiotic
bacteria that are toxic to the hemocytes of Galleria mellonella (Ffrench-Constant
and Bowen 2000). The purified insecticidal toxin from symbiotic bacteria when
given through feeding method killed the model insect Manduca sexta.
The genomes of X. nematophila and X. bovienii have been sequenced and
revealed several biosynthesis gene clusters involved in the biosynthesis of second-
ary metabolites, namely, benzylidene acetone, iodinine, phenethylamides, indole
derivatives, and complex compounds like xenocoumacins (XCNs) derived from
hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) sys-
tems, xenorhabdins, and xenorxides. Xenematide and xenortide insecticidal com-
pounds are produced by X. nematophila (Lang et al. 2008).
The genome of P. luminescens sp. laumondii strain TT01 has been completely
sequenced. Photorhabdus spp. produce various metabolites having antibacterial,
antifungal, cytotoxic, and nematicidal properties, which are apparently of mixed
peptide–polyketide origin. P. luminescens TT01 was analyzed to assign the func-
tional relationship with PKS. Many hypothetical proteins which are presented in
this organism have shown homology to PKS family. The mechanism involved in
biosynthesis and regulation of potential metabolites with insecticidal properties is a

vital process in nematode–bacteria (Razia et al. 2010).
Use of Photorhabdus and Xenorhabdus genes encoding entomotoxins to create
transgenic plants for crop protection was proposed by Ffrench-Constant and Bowen
(1999). Members of both of these genera produce insecticidal proteins that are toxic
to a wide variety of lepidopteran insects. These toxic proteins provide new insights
for production of transgenic crop plants like that of transgenic Bt crops. As with
other bacterial toxins, the mutation rate of bacteria in a population may result in the
variation of the sequence of toxin genes. Preferably, the toxins are active against
Lepidoptera, Coleoptera, and Diptera (Hofte and Whiteley 1989).
15.3.5 Efficacy of EPNs as Biopesticides
Out of the one million known species of insects, about 15,000 of them are
considered as pests. Some of the lepidopteran, coleopteran, dipteran, orthopteran,
and homopteran species have become serious pests and are currently a major threat
to several crops. Due to the unavailability of successful biological control agents to
manage these pests, farmers mainly depend on chemical pesticides. However, the
most effective and persistent insecticides used to control soil dwelling insects are
banned. Exploration and field testing of the biological control agents of these pests
is one of the major thrust areas of research in agriculture, which can lead to the
development of the commercial biological control agents needed at present.
The use of nematodes for biological pest control began in the 1930s. Nematode
appears promising because it is effective against a large number of insects, includ-
ing Japanese beetles, root and vine weevils, fire ants, mole crickets, cutworms, and
potato beetles. Unfortunately, field evaluations have not been very effective so far
due to the lack of understanding of the conditions required which facilitate infes-
tation, including abiotic factors such as soil moisture, temperature, and solar
radiation, as well as biotic factors such as nematode strain, host stage and its
defenses, and insect’s behavioral challenge events. The proper identification and
characterization of nematode–bacteria are fundamental importance in an
EPN-based biocontrol perspective (Sicard et al. 2003).
EPNs are applied with substrates that are regularly treated with many other
agents, including chemical or biorational pesticides, soil amendments, and fertil-
izers. Depending on the agents, application timing, and physicochemical characters,
the nematodes may or may not interact with these other agents. EPNs appear to be
compatible with many, but not all, herbicides, fungicides, acaricides, insecticides,
nematicides, azadirachtin, Bacillus thuringiensis products, and pesticidal soaps.
Inorganic fertilizer may be compatible with nematodes for short-term inundative
pest control. Heterorhabditis tend to be more sensitive to physical challenges,
including pesticides, than Steinernema (Grewal et al. 2001). Various studies were
carried out where plant-boring insects have been controlled by injecting nematode
suspensions into the borer holes with sponges soaked with nematode suspensions
(Yang et al. 1993). For the control of banana weevil, a nematode suspension can be
placed into insect-attracting cuts in residual rhizomes of bananas.
15.4 Mass Production of Biopesticides
For the past few decades, agriculture is dependent on synthetic and/or organic
insecticides for crop protection. Today awareness has been directed toward natural
enemies such as predators, parasites, and pathogens. Yet none of the predators or
parasites can be mass produced and stored for long periods of time, like that of
synthetic insecticides because it must be produced under in vivo conditions.
Technology is being developed to achieve high rates of production, to increase
the efficacy of a biological agent making it highly toxic to the target organism, to
enhance their shelf life, and to deliver it to the farmer in an effective way.
Entomopathogens like fungi, viruses, and bacteria have been suggested as control-
ling agents of insect pests for over a century. More attention needs to be given to the
selection of broad-spectrum biopesticides and improvement in their production,
formulation, and application technologies.
15.4.1 Methods of Production of EPNs
EPNs are currently produced by in vivo or in vitro (solid and liquid culture)
techniques. Each method has its own advantages and disadvantages relative to
production cost, technical know-how required, economy of scale, and product
quality. In vivo EPN production is the appropriate method for field testing and
laboratory use at a small scale. It requires small amounts of resources like labor,
insect culture, and technical expertise. For commercial use in vitro liquid culture is
usually considered relative to other production methods, and it demands greater
capital investment and a higher level of technical expertise.
15.4.1.1 In Vitro Production of EPNs
Method to produce EPNs initiates axenic nematode eggs placed on a pure culture of
the symbionts. Fermenters like airlift fermenter are used for liquid culture into
which symbiotic bacteria are first introduced followed by the nematodes. Various
ingredients for liquid culture media have been used, viz., soy flour, yeast extract,
canola oil, corn oil, thistle oil, egg yolk, casein peptone, milk powder, liver extract,
and cholesterol. Incubation time varies with the media and species being used and
may be as long as three weeks, although many species can reach maximum IJ
production in two weeks or less. Factors affecting yield of in vitro liquid cultures in
case of both Steinernematids and Heterorhabditids include the lack of nutritional
factors, aeration, CO2, lipid content, and temperature. The maximum yield of
nematodes in liquid culture fluctuates based on their life cycle and reproductive
biology.
15.4.1.2 In Vivo Production of EPNs
The general approach to in vivo culture is a two-dimensional system that relies on

production in trays and shelves (Ehlers and Shapiro-Ilan 2005). In vivo production
yields vary greatly among insect hosts and nematode species. The wax moth,
G. mellonella, is the insect of choice for in vivo production. It is produced in
many parts of the world as source of fish bait and bird food (Costa et al. 2007). The
silkworm is highly susceptible to EPNs and is a potential host for multiplication of
nematodes (Goldsmith et al. 2004). The most common insect hosts used for
laboratory and commercial EPN culture are Wax Moth (G. mellonella) and the yellow
mealworm (Tenebrio molitor), whereas other hosts which have been studied include
the navel orangeworm (Amyelois transitella), tobacco budworm (Heliothis virescens),
cabbage looper (Trichoplusia ni), pink bollworm (Pectinophora gossypiella), beet
armyworm (Spodoptera exigua), corn earworm (Helicoverpa zea), gypsy moth
(Lymantria dispar), house cricket (Acheta domesticus), etc. In vivo production
yields vary greatly among insect hosts and nematode species used, and the most
efficient and cost-effective host would be promoted for use by farmers.
Factors affecting yield of in vivo culture especially are nematode dosage and
host density. Crowding of hosts can lead to oxygen deficiency or increase of
ammonia. Thus, optimization of host density and inoculation rate of IJs for max-
imum yield is recommended. Infection efficiency and yield of IJs can be affected by
inoculation method. Approximately 25–200 IJs per insect are sufficient (depending
on nematode species and method of inoculation), whereas higher rates are generally
needed for the yellow mealworm, T. molitor (100–600 IJs per insect). The methods
of inoculation of nematodes include pipetting or spraying of nematodes onto a
substrate, immersion of insects in a nematode suspension, or applying the nema-
todes to the insect’s food. Environmental factors including temperature, aeration,
and moisture can affect the in vivo yield.
15.4.2 Application
Nematodes can be stored and formulated in different ways including the use of
polyurethane sponges, water-dispersible granules, vermiculite, alginate gels, and
baits. Formulated EPNs can be stored for 2–5 months depending on the nematode
species and storage media and conditions. Unlike other microbial control agents
(fungi, bacteria, and viruses), EPNs do not have a fully dormant resting stage, and
they use their limited energy during storage. The quality of the nematode product
can be determined by nematode virulence and viability assays, age, and the ratio of
viable to nonviable nematodes.
EPNs can be applied using nearly all agronomic or horticultural ground equip-
ment including pressurized sprayers, mist blowers, and electrostatic sprayers or
aerial sprayers (Shapiro-Ilan et al. 2006). The application equipment used depends
on the cropping system, and in each case there are a variety of handling consider-
ations including volume, agitation, nozzle type, and pressure. It is important to
ensure adequate agitation during application. For small-plot applications, water
cans or backpack sprayers can be used. When nematodes are applied to larger plots,
a suitable spraying apparatus such as a boom sprayer should be considered.
Conceivably, other methods such as through Microjet Irrigation Systems, subsur-
face injection, or baits could also be used. Enhanced efficacy of EPN applications
can be facilitated through cadaver application. Bait formulations and insect host
cadavers can enhance EPN persistence and reduce the quantity of nematodes
required when compared to liquid medium (Shapiro-Ilan et al. 2012). An overview
of the commercial use of EPNs is tabulated in Table 15.1.
Direct exposure to sunlight (UV light) can be minimized by applying infective
juveniles early in the morning or in the evening. EPNs should be prepared for field
application no earlier than 1 h ahead of application time. If nematodes are in a liquid
suspension, shake them well before use; if they are on a sponge, soak the sponge in
water; and if nematodes are in vermiculite, add the vermiculite–nematode mixture
directly to water allowing their dispersion. Mass production of these nematodes in
liquid media has become a major challenge for commercialization. A better under-
standing of both the nematode and its bacterial symbiont in liquid culture might
increase the chance of success.
Table 15.1 Commercial use of entomopathogenic nematodes (EPNs) Steinernema and

Heterorhabditis and insect pest
EPN species Major insect pest
S. glaseri White grubs (scarabs, Japanese beetle, Popillia sp.)
S. kraussei Black vine weevil, Otiorhynchus sulcatus
S. carpocapsae Cutworms, armyworms, black vine weevil, peach tree borer
S. feltiae Fungus gnats(Bradysia spp.), western flower thrips
S. scapterisci Mole crickets
S. riobrave Citrus root weevils
H. bacteriophora White grubs, cutworms, black vine weevil, flea beetles, corn rootworm
H. marelatus White grubs (scarabs), cutworms, black vine weevil
H. indica Fungus gnats, root mealybug, grubs
H. megidis Weevils
15.5 Conclusion
Research and development in the area of production of biological insecticides

magnetize extremely modest financial support compared that of chemical pesti-
cides. In addition to scientific studies, regulatory policies of the government should
aim at supporting further introduction of EPN-based products into biological
control practices, and the government should take measures to make the farmers
aware of EPNs as natural insect killers. EPNs are well adapted to their native habitat
and hosts through natural selection; however, in the near future, additional useful
traits could be established in their genome to make them even more competent
against insect hosts in different environments. With advancements, EPNs might
serve to reduce chemical insecticide inputs and add to environmentally sustainable
increase of crop yields.
References
Boemare NE, Akhurst RI, Mourant KG (1993) DNA relatedness between Xenorhabdus spp.
(Enterobacteriaceae), symbiotic bacteria of entomopathogenic nematodes, and a proposal to
transfer Xenorhabdus luminescens to a new genus, Photorhabdus gen. nov. Int J Syst Bacteriol
43:249–255
Costa JR, Dias RJP, Morenze MJF (2007) Determining the adaptation potential of multiplication
of a Heterorhabditis sp and Steinernema carpocapsae (Rhabditidae::Heterorhabditidae and
Steinernematidae) in larvae of Alphitobius diaperinus (Coleoptera: Tenebrionidae) and Gal-
leria mellonella (Lepidoptera: Pyralidae). J Parasitol 102:139–144
Eidt DC, Thurston GS (1995) Physical deterrents to infection by entomopathogenic nematodes in
wire worms (Coleoptera: Elateridae) and other soil insects. Can Entomol 127:423–429
Endo BY, Nickle WR (1991) Ultrastructure of the intestinal epithelium, lumen and associated
bacteria in Heterorhabditis bacteriophora. Proc Helminthol Soc Wash 58:202–212
Ehlers RU, Shapiro-Ilan DI (2005) Mass production. In: Grewal PS, Ehlers RU, Shapiro-Ilan DI
(eds) Mass production. CABI Publishing, Wallingford, pp 65–78
Ffrench-Constant RH, Bowen D (1999) Photorhabdus toxins: novel biological insecticides.
Microbiology 2:284–288
Ffrench-Constant RH, Bowen DJ (2000) Insecticidal toxin complex proteins from Xenorhabdus
nematophilus: structure and pore formation. Cell Mol Life Sci 57:828–833
Gaugler R, Boush GM (1978) Effects of ultraviolet radiation and sunlight on the nematode
Neoaplectanu carpocapsae. J Invertebr Pathol 32:291–296
Gaugler R, Bednarek A, Campbell JF (1992) Ultraviolet inactivation of Heterorhabditid and
Steinernematid nematodes. J Invertebr Pathol 59:155–160
Gerritsen LJM, DeRaay G, Smits PH (1992) Characterization of form variants of Xenorhabdus
Grewal PS, Nardo DE, Marineide ABE, Aguillera M (2001) Entomopathogenic nematodes:
potential for exploration and use in South America. Neotrop Entomol 30:191–195
Grewal PS, Wang X, Taylor RAJ (2002) Dauer juvenile longevity and stress tolerance in natural
populations of entomopathogenic nematodes: is there a relationship? Int J Parasitol
32:717–725
Goldsmith M, Toru S, Hiroaki A (2004) The genetics and genomes of silkworm Bombyx mori.
Gupta AP (1991) Insect immunocytes and other hemocytes: roles in cellular and humoral immu-
nity. In: Gupta AP (ed) Immunology of insects and other arthropods. CRC, Boca Raton, pp
19–18
Gupta AP (2002) Immunology of invertebrates: cellular. In: Encyclopedia of live sciences. Wiley/
Nature Publishing Group, London, p 9
Hofte H, Whiteley HR (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol
Rev 53:242–255
Hummel RL, Walgenbach JF, Barbercheck ME, Kennedy GG, Hoyt GD, Arellano C (2002)
Effects of production practices on soil-borne entomopathogens in Western North Carolina
vegetable systems. Environ Entomol 31:84–91
John MG, Wall DH, Behan-Pelletier VM (2006) Does plant species co-occurrence influence soil
mite diversity? Ecology 87:625–633
Kanga FN, Waeyenberge L, Hauser S, Moens M (2012) Distribution of entomopathogenic
nematodes in Southern Cameroon. J Invertebr Pathol 109:41–51
Kaya HK, Aguiller MM, Alumai A, Choo HY, De la Torre M, Fodor A, Ganguly S, Hazır S,
Lakatos T, Pye A, Wilson M, Yamanaka S, Yang H, Ehlers R-U (2006) Status of
entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions
of the world. Biol Control 38:134–155
Koppenhfer AM, Kaya HK, Taormino S (1995) Infectivity of entomopathogenic nematodes
(Rhabditida: Steinernematidae) at different soil depths and moistures. J Invertebr Pathol
65:193–199
Kung S, Gaugler R, Kaya HK (1990) Influence of soil, pH and oxygen on persistence of
Steinernema spp. J Nematol 22:440–445
Lang G, Kalvelage T, Peters A, Wiese J, Imhoff JF (2008) Linear and cyclic peptides from the
entomopathogenic bacterium Xenorhabdus nematophilus. J Nat Prod 71:1074–1077
Lewis EE, Campbell J, Griffin C, Kaya H, Peters A (2006) Behavioral ecology of
entomopathogenic nematodes. Biol Control 38:66–79
Millar LC, Barbercheck ME (2002) Effects of tillage practices on entomopathogenic nematodes in
a corn agroecosystem. Biol Control 25:1–11
Nguyen KB, Smart GC (1990) Steinernema scapterisci n. sp. (Steinernematidae: Nematoda). J
Nematol 22:187–199
Poinar GO (1990) Taxonomy and biology of Steinernematidae and Heterorhabditidae. In:
Gaugler R, Kaya H (eds) Entomopathogenic nematodes in biological control. CRC, Boca
Raton, pp 23–61
Puza V, Mracek Z (2007) Natural population dynamics of entomopathogenic nematode
Steinernema affine (Steinernematidae) under dry conditions: possible nematode persistence
within host cadavers? J Invertebr Pathol 96:89–92
Razia M, Raja KR, Padmanaban K, Sivaramakrishnan S, Chellapandi P (2010) A phylogenetic
approach for assigning function of hypothetical proteins in Photorhabdus luminescens subsp.
laumondii TT01 genome. J Comp Sci Syst Biol 3:21–29
Sicard M, Le Brun N, Pages S, Godelle B, Boemare N, Moulia C (2003) Effect of native
Xenorhabdus on the fitness of their Steinernema host: contrasting types of interactions.
Parasitol Res 91:520–524
Shapiro-Ilan DI, Bruck DJ, Lacey LA (2012) Principles of epizootiology and microbial control. In:
Vega FE, Kaya HK (eds) Insect pathology, 2nd edn. Academic, San Diego, pp 29–72
Shapiro-Ilan DI, Gouge DH, Piggott SJ, Patterson FJ (2006) Application technology and environ-
mental considerations for use of entomopathogenic nematodes in biological control. Biol
Control 38:124–133
Shapiro-Ilan DI, Gouge D, Koppenhofer A (2002) Factors affecting commercial success: case
studies in cotton, turf and citrus. In: Gaugler R (ed) Entomopathogenic nematology. CBI
Publishing, Oxon, pp 333–356
Thomas GM, Poinar GO (1979) Xenorhabdus gen. nov., a genus of entomopathogenic
nematophilic bacteria of the family Enterobacteriaceae. Int J Syst Bacteriol 29:352–360
Uribe-Lorio L, Mora M, Stock SP (2005) First record of entomopathogenic nematodes

(Steinernematidae and Heterorhabditidae) in Costa Rica. J Invertebr Pathol 88:226–231
Wang J, Bedding RA (1996) Population development of Heterorhabditis bacteriophora and
Steinernema carpocapsae in the larvae of Galleria mellonella. Fund Appl Nematol
19:363–367
Yang H, Zhang G, Zhang S, Jian H (1993) Biological control of tree borers (Lepidoptera:
Cossidae) in China with the nematode Steinernema carpocapsae. In: Bedding R, Akhurst R,
Kaya H (eds) Nematodes and the biological control of insect pests. CSIRO Publications, East
Melbourne, pp 33–40
Chapter 16
An Overview of Some Culture Collections
of Entomopathogenic Microorganisms
in the World
16.1 Introduction
Pure cultures of microorganisms are becoming increasingly important in diverse

fields such as agriculture, medicine and industrial biotechnology. Culture collection
centres play an important role in making these pure cultures available for scientific
and industrial use. One of the functions of the culture collection facility is to act as a
repository. Receipt, verification, preservation, maintenance and accession of cul-
tures are the main activities undertaken by such a facility. Standard protocols are
followed to conserve microbial strains under optimal conditions. Most commonly
microbial stock cultures are preserved in glycerol, mineral oil, sterile distilled water
or in lyophilised form. In addition, cryopreservation of authentic strains is also
carried out in liquid nitrogen. Information about the cultures maintained at the
culture collection centre is maintained in the form of inventories and as online
databases which could be of use also to the ones who seek services from them.
Apart from these activities, the collection centres also provide various services to
the academic and industrial communities. These include morphological and molec-
ular identification of microbial cultures, deposition and accession of microbial
cultures and supply of authentic microbial strains to academia, research institutions
and industries. The culture collection facilities are also centres of active research. A
few of the culture collection centres for microbes in general and specifically
entomopathogenic microorganisms have been detailed below. The main aim of
the present chapter is to introduce a few of the entomopathogen culture collection
centres around the world to the scientists who want to start with research on
entomopathogens, enabling them to acquire pure and authentic cultures of
entomopathogens.
K.S. Sree (*) • A. Varma


DOI 10.1007/978-3-319-14499-3_16
16.2 American Type Culture Collection
American Type Culture Collection (ATCC) is a non-profit biological resource

centre (BRC) and is a private research organisation. The centre aims at the
acquisition, authentication, production, preservation, development and distribution
of reference standards for microorganisms, cell lines and other research materials in
the field of life sciences. A group of scientists realised the necessity of a collection
of microorganisms with a purpose to serve the scientists worldwide, and this
resulted in the establishment of ATCC in 1925.
ATCC possesses a broad collection of biological research materials, which
includes microorganisms, cell lines, molecular genomics tools and bioproducts.
More than 3,400 human, animal and plant cell lines are available at ATCC. The
collection of molecular genomics includes eight million cloned genes from human,
mouse, soybean, rat, monkey, zebrafish and several disease vectors. In addition,
collections of protozoans, yeasts and fungi having more than 49,000 yeast and
fungal strains and 2,000 strains of protists are maintained at ATCC. The collection
of microorganisms includes over 18,000 strains of bacteria, 2,000 different animal
viruses and 1,000 plant viruses. Entomopathogenic viruses, bacteria and fungi form
a part of the collection of microorganisms at ATCC (cf. ATCC website).
16.3 World Federation for Culture Collections
The World Federation for Culture Collections (WFCC) is a multidisciplinary

commission of the International Union of Biological Sciences (IUBS) and a feder-
ation within the International Union of Microbiological Societies (IUMS). The
centre is involved in the collection, authentication, maintenance and distribution
of microbial and cell cultures. It aims at promoting and supporting the setting up of
new culture collections and their related services, providing liaison and establishing
an information network between the collection centres and their users, organising
workshops and conferences, bringing out publications and newsletters and also
ensuring the long-term growth of important collection centres.
The WFCC is a pioneer in the development of a database, WFCC World Data
Centre for Microorganisms (WDCM), on culture collection centres around the
world for international use which is an important resource for information on all
microbiological activities. WDCM also acts as a centre for data activities among the
members of WFCC. The National Institute of Genetics (NIG) at Japan maintains
this database and has close to 476 culture collections from 62 countries as records.
The data on the organisation, management, services and scientific interests of the
collections are stored in this database.
The Culture Collections Information Worldwide which includes CCINFO and
STRAIN is a database management system for culture collections in the world.
STRAIN is a database which is composed of the list of holdings present in the
16 An Overview of Some Culture Collections of Entomopathogenic Microorganisms. . . 331
culture collection centres around the world, and CCINFO maintains a directory of
all the culture collections of the world which are registered. The information on
671 culture collections located in 70 countries and regions is provided in the
database, including 2,421,998 microorganisms (bacteria, 1,034,647; fungi,
706,083; virus, 37,666) (cf. WFCC website).
16.4 Microbial Type Culture Collection
The Microbial Type Culture Collection (MTCC) and Gene Bank is a national
facility at the Institute of Microbial Technology (IMTECH) located in Chandigarh,
India. It was established in 1986 as a joint effort of the Department of Biotechnol-
ogy and the Council of Scientific and Industrial Research, Government of India. It
is a registered member of the WFCC and WDCM. Similar to other culture collec-
tion centres, it aims at serving as a depository, at supplying authentic microbial
strains and at delivering appropriate services to the scientific community affiliated
to universities, research institutions and industries.
The MTCC, on 4 October 2002, was recognised as an International Depositary
Authority (IDA) by the World Intellectual Property Organization (WIPO) located at
Geneva, Switzerland. It is the first IDA established in India. Under the Budapest
Treaty, the deposit of microorganisms is recognised in 55 member countries so as to
fulfil the requirements of patent procedures in all these countries.
Currently, the MTCC houses five sections. These sections include the cultures of
actinomycetes, bacteria, fungi, yeasts and plasmids. Collectively they hold more
than 9,000 cultures. In general, the cultures are preserved under liquid nitrogen and
by freeze-drying. The fungal strains are also preserved in mineral oil. In order that
the cultures continue to represent the strains which were originally deposited,
viability of the strain and some other key characteristics of the organisms are
assessed periodically, although some specific properties like secondary metabolite
production, degradation of specific compounds and so on are not assessed on a
normal basis. It also maintains a database which provides relevant information on
the microbial strains available at the MTCC (cf. MTCC website).
16.5 National Fungal Culture Collection of India
India hosts a vast diversity of fungal resources. Moreover, India has one of the
largest fungal biodiversity gene pools in the world. Since decades, mycologists
have been researching on fungi and have reported and described new and interest-
ing fungi from India. Long-term preservation of fungal strains in the form of pure
cultures allows the availability of these fungal strains for scientific and industrial
research.
The Agharkar Research Institute at Pune maintains the National Fungal Culture
Collection of India (NFCCI) which is a unique national facility established by the
Department of Science and Technology, Government of India. It is also a registered
with the WFCC and the WDCM. This facility aims at conserving fungal strains as
genetic resource pools for use in future scientific research and to provide services to
academia and industry. Over 2,800 fungal strains belonging to different groups are
included in the NFCCI repository (cf. NFCCI website).
16.6 Agricultural Research Station Collection

of Entomopathogenic Fungal Cultures
The Agricultural Research Station Collection of Entomopathogenic Fungal Cul-

tures (ARSEF), established in the early 1970s, is one of the culture collections
worldwide which is concentrated mainly on entomopathogens. This culture collec-
tion centre aims at providing fundamental support for research on the fungal
pathogens of invertebrates in both basic and applied fields.
The ARSEF culture collection centre is run by the Biological Integrated Pest
Management Research Unit of the USDA Agricultural Research Service. ARSEF is
one of the largest germplasm collections of fungal pathogens of invertebrates
globally. ARSEF is also registered with the WFCC and WDCM. The ARSEF
cultures are preserved as cryogenic stocks in liquid nitrogen. Lyophilised forms
of the cultures which can tolerate lyophilisation are also available.
The culture collection holds fungal strains which are isolated from insects, other
arthropods and nematodes. This facility renders general research resources for the
isolation, collection, preservation and distribution of these fungal strains. The
collections served to distribute the fungal strains to researchers worldwide on
request. It also seeks to acquire fungal strains. Fungal systematics, fungal cytology,
methodologies for long-term preservation of fungal germplasm and pathobiology
are the areas of basic research which are directly associated with this culture
collection. The slide collection and the collection of herbarium specimens at
ARSEF extend support to researchers in the field of fungal taxonomy and also aid
in the diagnoses and identification of fungal pathogens associated with inverte-
brates. ARSEF also provides free-of-charge services for the identification of fungal
cultures and follows up-to-date fungal taxonomic classifications, and the curator,
Richard A. Humber, of the culture collection centre is also keen at receiving any
information about nomenclatural or taxonomic changes or possible misidentifica-
tions involving any ARSEF strains. All these efforts are made with an intention to
provide accurately identified and pure fungal cultures to the researchers in this field
(cf. Humber, ARSEF website).
16 An Overview of Some Culture Collections of Entomopathogenic Microorganisms. . . 333
16.7 University of California Davis Nematode Collection
The University of California Davis Nematode Collection (UCDNC) is one of the

largest collections of nematodes worldwide. It is a very comprehensive collection
of various types of nematodes including freshwater, marine, free-living soil and
plant-parasitic nematodes. The collection includes samples in the form of slides and
wet specimens from more than 90 countries including 47 of the USA, representing a
wide geographical area. The nematode collection largely conserves original spec-
imens of the newly described species, research-associated voucher specimens and
specimens which were collected from different parts of the world. The slide
collection of the type specimens, ranging over 11,000 specimens, includes 3,454
primary types representing 930 species and 14 of the 18 recognised orders. More
than 53,000 specimens representing 2,221 species belonging to 16 orders are
included in the general slide collection. These specimens are of utmost use to
researchers in the field of comparative morphology, taxonomy, ecology, evolution,
biological control and agriculture. The UCDNC, having a huge reference source of
identified specimens, is extremely useful for the precise identification of nematode
specimens, in turn helping the nematology-related research. The huge catalogued
wet collection of the UCDNC is highly appreciable. This includes 5,717 samples
from 96 localities, out of which 926 are from the USA and 4,791 are from the other
parts of the world. In collaboration with the laboratory of Dr. Harry Kaya, UCDNC
maintains live cultures of entomopathogenic nematodes. This collection is currently
represented by 21 species and 30 isolates from the USA and other locations
worldwide (cf. UCDNC website).
With the ever-increasing number of microbes being identified and with the
advancements in molecular and genetic tools, the role of culture collection centres
in maintaining, identification and supply of microbial cultures for academic and
industrial use is gaining importance. Establishment of more specific culture collec-
tion centres with the financial support of the government or non-profit private
organisations is being looked forward to.
Acknowledgements KSS is grateful to the Science and Engineering Research Board, Govern-
ment of India, for support through the fast-track Young Scientist project.
References
ARSEF website: http://www.ars.usda.gov/News/docs.htm?docid¼12125. Date last accessed:

12.01.2015
ATCC website: http://www.atcc.org/en/Products/Collections/Microbiology_Collections.aspx.

Date last accessed: 12.01.2015
Humber RA: http://vivo.cornell.edu/display/individual10325. Date last accessed: 12.01.2015
MTCC website: http://mtcc.imtech.res.in/. Date last accessed: 12.01.2015
NFCCI website: http://nfcci.aripune.org/. Date last accessed: 12.01.2015
UCDNC website: http://nematology.ucdavis.edu/about/facility/collection.php. Date last accessed:
12.01.2015
WFCC website: http://www.wfcc.info/. Date last accessed: 12.01.2015
Index
A development, transgenic crops (see

Acid phosphatase, 211, 215–216 Transgenic crops development, Bt
Acontia graellsii, 265 toxins)
AgMNPV. See Anticarsia gemmatalis immunohistochemical detection, toxin
nucleopolyhedrovirus (AgMNPV) receptors, 75
Agricultural Research Station Collection of kurstaki strain HD1, 54
Entomopathogenic Fungal Cultures life cycle, 71
(ARSEF), 332 mechanism of toxicity, 71
Agrotis ipsilon, 265–266 Mediterranean flour moth, 54
Alabama argillacea, 260–261 mode of action, 58–59
Alkaline phosphatase, 211–212, 215–216 receptors expression, cell lines, 76
American Type Culture Collection resistance management strategies, 66–67
(ATCC), 330 RNAi, 76–77
Anomis flava, 261 sotto disease, 54
Anticarsia gemmatalis nucleopolyhedrovirus strains and target insects, 55
(AgMNPV), 16–17 structural domains, 71
ATCC. See American Type Culture Collection structure, Bt δ-endotoxins, 57–58
(ATCC) toxin-affinity chromatography, 74–75
toxins
activity, 53–54
B as bioinsecticide spray, 60–61
Bacillus thuringiensis (Bt), 139 genes, 55–57
binding blot overlay mosquitoes control, 61
iterative nonlinear curve-fitting transcriptome
programs, 72–73 Bt var. israelensis (Bti), 80
one-dimensional electrophoresis, 73 cadherin and aminopeptidase N
procedures, 73 receptors, 79
radioligand binding assay, 72 chemical insecticides, 78–79
samples, 73 Colorado potato beetle (Leptinotarsa
two-dimensional gel electrophoresis, 74 decemlineata), 80
Western blot, 73 diamondback moth (Plutella xylostella),
biological control agent, 53 79–80
CADP, 71–72 enzyme ALP, 79
Cry proteins, 71 massive sequencing, 78
Cry toxins, 54, 72, 81 mechanism of action, Cry toxins, 79

DOI 10.1007/978-3-319-14499-3
336 Index
Bacillus thuringiensis (Bt) (cont.) structure, 174, 176

microarray experiments, Tenebrio virulence factor, 226
molitor, 79 Bassianolone, 227
M. sexta midgut, 80 BCAs. See Biological control agents (BCAs)
programs, 78 Beauveria bassiana (B. bassiana), 140–141
rice borer worm (Chilo suppressalis), 80 biopesticide, 220
RNA-Seq analysis, 77–78 bioremediation, heavy metals, 223
types, 53 Cordyceps, 219
VIP, 53 entomopathogenic vs. endophytic nature, 221
Bacteria formulation types
Bt cotton, 5 conidia mixing, metabolites, 222
B. thuringiensis, 4 endophytic, 222–223
cyt toxins, 4 extracted protein, 222
Photorhabdus, 5 host ranges, 220
Vip toxins, 4 internal machinery, fungal bioformulation
Baculovirus G-protein signaling (BbRGS1), 230–231
bio-pesticide development, 12 hydrophobins, 228–229
chemical control, 11, 13 mannitol metabolism and multistress
classifications tolerance, 229–230
double-stranded DNA (dsDNA), 13 MAP kinase activity, 230
EM observation, 13, 14 superoxide dismutase, 227–228
GVs (see Granuloviruses (GVs)) secondary metabolites
NPVs (see Nucleopolyhedroviruses bassiacridin, 224
(NPVs)) bassianolide, 226–227
occlusion/inclusion bodies (OBs), 13 bassianolone, 227
POBs, 15 beauvericin, 225–226
viral forms during postinfection, 14–15 multifarious roles, 223, 224
commercial based products, pest strain improvement, 231–232
management, 16–17 Beauvericin(s)
components, 13 acetyl coenzyme A, 178
farmers in Asia, 12 analogues, 174, 177
insect pests control, agriculture and animal tissues detection, 178
forestry, 12 antibacterial activity, 225
IPM, 11–12 antifungal activity, 226
isolation and characterization antitumor activity, 225
biological, 19–20 antiviral activity, 226
chemicals effect, 26–28 besides, 225
molecular-level identification and insecticidal effects, 177
evaluation, 28–31 mycelial growth, 177
morphological, 18–19 peptide binds, 225
NPV efficacy, different storage structure, 174, 176, 177
conditions, 24–26 Beauveriolides
plant protection, 17 analogues, 178, 182
viral proteins, 20–24 structure, 178, 182
microbial pathogens, 12 Beauverolides
quantities, 13 analogues, 178, 180–181
storage, 13 structure, 178, 179
synthetic insecticides, 11 Biological control agents (BCAs)
Bassiacridin, 224 agro-ecosystem play, 2
Bassianolides Beauveria bassiana, 2
analogues, 174, 176 integrated pest management (IPM)
bona fide virulence factor, 226 strategies, 2–3
drastic reduction, 226 lepidopteran larvae, 2
Index 337
Biopesticides, mass production biosynthesis precursors, 215

application, 324–325 impressive changes, 207–208
EPNs materials and methods
in vitro, 323–324 biochemical profile, 211–212
in vivo, 324 biosynthesis precursors, 211
chemicals, 208
fungal strains, 208–209
C RSM, optimization, 209, 210
Cadherin-like protein (CADP), 71–72 statistical analysis and modeling, 209
Chitinase and chitin labeling, TEM in vitro production and recovery, dtx A
chitinase activity, 250 and E, 209–211
germinated conidia, 250, 251 M-19 strain on supplemented medium,
hydrolases, 251 215–216
and NAG, 252 optimal conditions
Cicadapeptins, 170 ANOVA, 213, 214
Conoideocrellides and paecilodepsipeptides CCD experiments, 212
analogues, 179, 183 surface plots, 212–214
structure, 179, 182 optimization, media components, 208
Cordyceps bassiana, 219 predominant forms, 207
Cordycommunin, 179, 184 RSM, 216
Cordyheptapeptide tetanic paralysis, 207
analogues, 194, 197 Cyclopeptides
antimalarial activity, 194 cordyheptapeptide, 194–195, 197
cytotoxicity, 195 cyclosporines, 193–196
structure, 194, 197 Cyclosporines
Cotton defoliators management analogues, 193, 196
bio-limiting factor mosquito larvae, 194
factors, 256 structure, 193, 195
world and symptoms, 256, 257–258 Cydia pomonella granulovirus (CpGV), 277–278
entomopathogens (see Entomopathogens)
grasshoppers, 259
mechanical wounding, 258 D
natural environment, 258 Destruxins
pesticides, 256–258 analogues, 182, 185–186
production, country report, 255, 256 chemical structure, 182, 184
strategy, 267 cyclohexadepsipeptidic mycotoxins, 179
CpGV. See Cydia pomonella granulovirus hemocoel injection, 183
(CpGV) morphology and function, 184
Culicinins, 171, 172
Cyclodepsipeptides
bassianolides, 174, 176 E
beauvericins, 174, 176–178 Efrapeptins
beauverolides and beauveriolides, 178–179 analogues, 171, 173
conoideocrellides and breast cancer cells, 172
paecilodepsipeptides, 179, 182, 183 cytotoxicity, 172
cordycommunin, 179, 184 insecticidal activity, 171
destruxins, 179, 182–186 structure, 171, 172
enniatins, 184, 186–187 Egyptian cotton leafworm, 124
hirsutellides and hirsutides, 187, 190 Endophytic beauveria, 222–223
isariins, isaridins and isarolides, 187, Enniatins
190–192 analogues, 184, 188–189
serinocyclins, 192, 193 molecular mechanism, 186–187
verticilide, 192–194 proline residue, 186
Cyclodepsipeptidic destruxin recovery spruce budworm larvae, 186
amino acids, 207 structure, 184, 186
338 Index
Entomogenous fungal NRPs bacteria (see Bacteria)

as biocontrol, lepidopteran pests, 195, 197 BCAs, 2–7
as immunosuppressant insecticides, 198 biological control, 2–3
molecular mechanism, 197–198 chemical insecticides, 2
pathogenic factor, 195 fungi, 5–6
Entomogenous fungi. See Non-ribosomal MTCC, 331
peptide synthetase (NRPS) nematodes, 6–7
Entomopathogenic fungi NFCCI, 331–332
Beauveria, 140–141 pathogenic microbes, 1
biological control standard protocols, 329
augmentation, 130 UCDNC, 333
classical, 129 viruses, 3–4
conservation, 130 WFCC, 330–331
insect pests, 127 Entomopathogenic nematodes (EPNs)
lepidopteran pests, 146 bacterial symbionts, 274–275
M. anisopliae, 140 as biocontrol agents
“white holo,” V. lecanii, 140 behavior, 318–319
biology and ecology, 132 distribution, 318
caterpillar, 146 insect host, 320–321
chitin fibrils, insect cuticle, 133 pH, 320
classification/systematic position, 128 soil and its moisture, 319
compatibility, 135–136 temperature, 319–320
disease-causing, 127 tillage, 321
dispersal/transmission, 128–129 UV source, 320
efforts, 146 as biological control agent
epizootics, 128 commercial production formulation, 295
Fusarium, 146 epigeal (soil surface) habitat, 301–305
genera, 127 foliar application, 299–301
genetic manipulation, 131 IPM and sustainable agriculture, 295
Hypocreales, 131 lethal obligatory parasites, insects, 295
infection process, 134 marketing, 295–296
insect immune responses, 134–135 nematode host-seeking strategy, 305
invasion, 133–134 success/failure of nematodes, 305–306
Isaria, 144–145 susceptibility, 296–299
isolation as biopesticides, 322–323
from insects, 132–133 in combination with Bt, 282–283
from soil, 133 efficacy against lepidopteran pest
“law of natural balance”, 140 corn, 281
Lecanicillium, 145 cotton, 281
life cycle of, 131 orchard trees, 276–280
M. anisopliae, 128 pests management in fruit bins, 281–282
Metarhizium, 141–143 turf, 281
microbial control, 127 vegetables, 280
modern exploration, 127 foraging strategy, 275–276
mycoinsecticides, 127, 128 Helicoverpa spp. control management
mycopathogens, 140 (see Helicoverpa spp.)
Nomuraea, 143–144 life cycle
secretion, 133 heterorhabditids, 316–317
spectacular epizootics, 140 steinernematids, 316
spores and transmission, 134 symbiotic bacteria, 317
toxicity, 133 Metarhizium (see Metarhizium)
Entomopathogenic microorganisms pathogenicity, 321–322
abiotic and biotic factors, 1 symbiotic nematode–bacteria pairs, 273
ARSEF, 332 ubiquity and insecticidal specificity, 273
ATCC, 330 Entomopathogens
Index 339
causing disease, 259–260 RTX-like alkaline protease, 108

and cotton pest management RVA technique, 108
Acontia graellsii, 265 toxin complexe (Tc), 106–107
Agrotis ipsilon, 265–266 TTSS, Photorhabdus and
Alabama argillacea, 260–261 Xenorhabdus, 106
Anomis flava, 261 toxins production, 92
grasshoppers, 266 Granuloviruses (GVs)
Helicoverpa zea, 261–262 based products, 16
mites, 266 bio-pesticides, 16
Spodoptera spp., 262–265 commercial insecticides, 16
Sylepta derogata, 265 detection, human pathogens, 26
EPN. See Entomopathogenic nematodes Epinotia aporema, 42
(EPNs) Erinnyis ello (cassava hornworm), 16
Erinnyis ello (cassava hornworm), 16 granulin, 42
Esterase, 212 host range, 17
isolation, 18
Lepidoptera order, 13
F microbial pesticides, 36
Fungi morphological characteristics, 13, 14
insect pest control, 5 occlusion bodies, 15
insect species, 5 Pieris rapae, 16
mechanical injury, 6 Plutella xylostella, 16
Fusarium, 146 products, 16
protection, apple and pear orchards, 16
small OBs, 13
G species, 17
Geotrichum candidum, 172 spraying cassava crops, 16
Gram-negative bacteria viral forms, 14–15
B. thuringiensis, 92 Grasshoppers, 266
control, pest species, 92 Green muscardine disease, 161
crop protection, 92 GVs. See Granuloviruses (GVs)
cytotoxin, digestive and neurotoxins, 92
enterobacteriaceae species, 92
insect virulence factors H
Photorhabdus (see Photorhabdus) Helicoverpa spp.
Pseudomonas (see Pseudomonas) common names, 289
Xenorhabdus (see Xenorhabdus) control
Yersinia, 104–105 biological, 294
Lepidoptera, 92 chemical, 293–294
pathogens, invertebrates cultural, 293
anti-invertebrate and anti-vertebrate ETL and population monitoring,
virulence factors, 108–109 pheromone, 292
antimycotic activity, 107 host-plant resistance, 293
apoptotic binary toxin Xax, 107 with nematodes, 306–307
“core genome” and “flexible genome”, crop production, 307
105–106 effectiveness, 307–308
genome data analysis, 105 geographic distribution, 289–290
mcf (makes caterpillar floppy) gene, IPM
107–108 programmes, 290
microarrays and software tools, 108 strategies, 295, 307
P. luminescens and P. asymbiotica microbial pesticides, 291
strains, 108 morphology and biology, 291–292
PVCs, 108 pest management strategies, 290
regions of genome plasticity (RGPs), 106 prevention, 308
340 Index
Helicoverpa spp. (cont.) M

protein resistance, transgenic toxin Bt MAP kinase activity (MAPK), 230
crops, 290–291 Metarhizium
soil-dwelling biocontrol agents, 291 characterization, 161
Trichogramma egg parasitoids, 291 description, 161
UV light, 291 distribution, 162
Helicoverpa zea, 261–262 egg hatching, 142
Heterorhabditids, 316–317 generalist vs. specialist
Hirsutellides, 187, 190 destruxin (dtx), 163–164
Hirsutides, 187, 190 genome and transcriptome, 162
Hydrophobins, 228–229 G-protein-coupled receptors, 163
host specificity, 162
M. acridum, 162
I M. anisopliae, 162
IDA. See International Depositary Authority mechanical kill of pest, 163
(IDA) plant fungal pathogens/endophytes, 162
Insecticidal toxin proteins, 283–284 proteases, 162–163
International Depositary Authority (IDA), 331 green muscardine disease, 161
Isaria farinosa and Isaria fumosorosea, 144–145 green muscardine fungus, 141
Isaridins M. acridum, 165
analogues, 187, 191 manipulation, host range, 164
structure, 187, 191 M. anisopliae, 141–142, 161–162
Isariins penetration, 142
analogues, 187, 191 susceptibility, 142–143
structure, 187, 190 Metarhizium anisopliae
Isarolides acid phosphatase, 211
analogues, 190, 192 alkaline phosphatase, 212
structure, 190, 191 esterase, 212
Microbial Type Culture Collection
(MTCC), 331
L Mites, 266
Lecanicillium, 145
Lepidoptera, 273–274
Lepidopteran pests N
A. thaliana, 110 National Fungal Culture Collection of India
biocontrol, 195, 197 (NFCCI), 331–332
Bt-derived proteins, 109 Neoefrapeptins
EPN efficacy analogues, 172, 175
corn, 281 Geotrichum candidum, 172
cotton, 281 structure, 172, 174
orchard trees, 276–280 NFCCI. See National Fungal Culture
pests management in fruit bins, 281–282 Collection of India (NFCCI)
turf, 281 Nomuraea (N. rileyi)
vegetables, 280 geographical isolation, 143–144
genetically modified (GM) crops, 109 S. frugiperda, 143
hemolymph-based toxicity, Mcf, 110 S. litura and H. armigera, 143
insecticidal protein, 111 in soybean, 143
oral and hemolymph-based toxic tomato, groundnut, cotton, black and red
activity, 110 gram, 143
patent applications, 109 Non-Bt soil microbe-derived insecticidal proteins
P. entomophila, 110–111 bacteria, 91
Photorhabdus and Xenorhabdus, 110 biotechnology, 89
PirAB binary toxins, 110 B. thuringiensis (Bt), 90–91
P. xylostella, 110 chemical insecticides, 89
soil-dwelling EPNs, 109 control, 112
Index 341
EPN, 90–91 safety and quality control, 32

genome analysis, 112 S. litura NPV, 34–35
gram-negative bacteria (see Gram-negative viral recovery procedures, 32
bacteria) NPVs, production technologies
Lepidopteran (see Lepidopteran pests) susceptible insect host, 31–32
microbial entomopathogens, 89 in vitro, 37–40
pest control programs, 89 in vivo, 32–36
transgenic crops protection, IPM, 90 NRPS. See Non-ribosomal peptide synthetase
Non-ribosomal peptide synthetase (NRPS) (NRPS)
chain peptides Nucleopolyhedroviruses (NPVs), 139
cicadapeptins, 170 A. californica and A. falcifera, 17
culicinins, 171, 172 AgMNPV, 16–17
efrapeptins and neoefrapeptins, Autographa californica and Anagrapha
171–174 falcifera, 16
cyclodepsipeptides (see biological characterization
Cyclodepsipeptides) HaNPV, 19, 20
cyclopeptides, 169, 193–195 HzNPV, 20
entomogenous fungal, 195, 197–198 LC50 values, 20–22
natural population, 169 MNPVs isolation, ICRISAT
NPVs. See Nucleopolyhedroviruses (NPVs) farms, 19, 20
NPVs, in vitro production characteristics, MNPVs, 18–19
bioreactors, 37 chemicals, effect of
commercial, 40 acetone, 28
fetal calf serum, 37 Bacillus cereus, 26
HaSNPV OBs, 38–39 bacterial contaminants, 26, 28
H. zea and H. armigera viruses, 38, 39 HaNPV, 26, 27
insect cell cultures, 37–38 L. dispar, 26
issues, quality control, 37 outcomes, 28
limitations, 39–40 screening, 26
lipid-supplemented environment, 37 S. littoralis, 26
low-cost culture media, 37 in China, 16
nuclear polyhedrosis infections, 38 diagnostic and quality control tests
process, 39 DAC, 41
Spodoptera litura, 39 detection and quantification, 42
NPVs, in vivo mass production effectiveness, viral insecticides, 40
advantages, 32 ELISA and Western blotting assays, 41–42
AmalNPV, 33 mass production, 40, 41
commercialization, products, 35–36 performance, viral biocontrol agents, 40
earlier and late instars larvae, 33–34 tools, 40
field collection, 32, 33 differentiation, 15–16
formulation, 32 efficacy, storage conditions
HaNPV, 32, 33 ICRISAT, 24
H. armigera, 34 period of time, 24
HzNPV, 34 variations, HaNPV storage, 24–26
ICRISAT, 33 Helicoverpa armigera, 15
in India, 35 host range, 17
initial rearing system, 32 ICRISAT farms, 18
inoculation, 33 infected larvae, 18
inoculum-imbibing method, 34 integrated pest management (IPM), 11
insect pest control, 32 L. dispar MNPV formulations, 17
laboratory-colonized insects, 32–33 Lepidoptera, 13
large-scale rearing of insects, 33 Mamestra brassicae MNPV, 17
LC50 values, 34 molecular characterization
optimal virus, 32 AcMNPV and BmNPV, 30
polyhedra, 34 AmalNPV, 29
342 Index
Nucleopolyhedroviruses (NPVs) (cont.) P

commercial insecticide preparations, 29 Paecilodepsipeptides
detection and identification, 30 analogues, 179, 183
DNA examination, 29 structure, 179, 183
geographical isolation, 31 Pesticides
HaMNPV and MbMNPV, 30 agricultural chemicals, 256
HaNPV and SlNPV polyhedrin, 29 cotton pest management, 258
ICRISAT, 29 defoliator management, 256
intrinsic differences, 28 Photorhabdus
M. brassicae, 29 gram-negative, bioluminescent and motile
microscopic and serological tools, 28 bacteria, 93
nucleocapsids, 29 hemolysins/hemagglutinin-related
PCR–RFLP analysis, 30 proteins, 99
phylogenetic status, 28 Heterorhabditis nematodes, 93
polyhedrin/granulin, 29–30 human pathogen, P. asymbiotica, 93
restriction endonuclease (REN) infective juvenile (IJ) nematode, 93
analysis, 29, 30 insecticidal toxin (Pit), 98
similarity and variability, 31 insect-related binary toxins, PirA/B, 96–97
strain identification, 29 makes caterpillars floppy (Mcf1 and Mcf2)
morphological characteristics, 13, 14 toxins, 97
plant protection, 17 Phe tRNA, 94
POBs, 18, 19 P. luminescens, 93–94
polyhedra, 15 P. temperata, 93
postinfection, 14–15 toxin complexe (Tc), 94–96
production technologies (see NPVs, Txp40 toxin, 97–98
Production technologies) virulence cassettes (PVCs), 98–99
proteins analysis Plutella xylostella, biocontrol with Beauveria
GAU sample, 23 bassiana
ICRISAT, 22 appressoria
lepidopteran NPVs, 22 appressorium, cuticle, 245, 246
polyhedrin/granulin, 20, 22 and germ tube, 245, 247
polypeptides, SNPVs, 23–24 host penetration, 245
replication, 22 attachment
SDS-PAGE, 22–23 conidium, 240, 242
stable, 22 cross section, cuticle, 240, 241
structure, 22–23 superficial epicuticle, 240
regulations, poor products, 43 TEM, 240–242
research, 42 deleterious effects, 237
SEM images, OBSs, 13, 14 enzyme activities
size and shape, occlusion bodies, 15–16 chitinase and chitin labeling, TEM,
SNPV and MNPV, 13 249–252
SPOD-X™, 16 superficial chitinase profiles, SEM, 249
germination
conidial and appressorial formation,
O 242, 243
Occlusion-derived virions (ODVs), 3–4 SEM, 243, 244
Orchard trees, EPN efficacy against metabolites, 252
lepidopteran pest penetration
alder and sycamore, 280 entomopathogenic fungi, 246
apple and pear trees, 277–278 invasion, cuticle, 248, 250
grape, 279 mucilage and appressorial structure,
litchi, 279 248, 249
nuts, 278 rapid growth, 248, 251
peach, 278–279 reveal environmental effect, 239
strawberry, 279 virulence assay, 238, 239
Index 343
Poly-occlusion bodies (POBs), 15 predators and parasitoids, 138–139

Predators and parasitoids, 138–139 prophylactic, 136–137
Pseudomonas pests, subtropical and tropical agricultural
crop protection, 102 crops, 124
distribution, 102 S. exempta, 124
hemocoel-based toxicity, P. fluorescens, S. exigua, 125
102, 103 S. frugiperda, 125
P. entomophila, 102, 103 S. littoralis, 125
P. syringae, 102 S. littoralis (Boisd.), 262–264
root-associated bacteria, 102 S. litura (Fab.), 264–265
versatile gram-negative bacteria, 102 S. litura (Fabricius) (see Spodoptera litura
(Fabricius))
Steinernema and Heterorhabditis nematodes,
S 274–275
Serinocyclins Steinernematids, 316
analogues, 192, 193 Superoxide dismutase, 227–228
structure, 192, 193 Sylepta derogata, 265
Serratia spp. Symbiotic bacteria, 317
amber disease-associated plasmid, 104 as biopesticide, 283–284
antifungal activity, endophytic pathogenicity, 321–322
rhizobacteria, 103
intestine, S. plymuthica, 103
isolation, grassland soils, 103 T
lepidopteran hosts, S. marcescens, 103 Transgenic crops development, Bt toxins
S. entomophila and S. proteamaculans, 104 advantages, 61
sep gene orthologues, 104 Bt var. tenebrionis, 62
S. litura (Fabricius), 124 chemical pesticides, 62
Spodoptera litura (Fabricius) codon modifications, 62
description, 125 commercial crops against insect pests, 63
developmental stages, 126–127 corn hybrids, cry1Ab gene, 62
dispersion and oviposition, host plants, 125 cotton crop, USA, 64–65
distribution, 125 cotton varieties, 62
egg masses, 126 cry3A gene, Monsanto’s new leaf potato
fecundity, 126 variety, 62
larvae of S. littoralis, 125 cry1F expression, 64
subtropical and tropical range, 125 cultivation area, 64, 65
tobacco cutworm, 124 European corn borer, 65–66
Spodopteran pests management field trials approval, 64
biological control, 123 gene construction, 62
chemical pesticides, 123–124 generation, GM crops, 62–63
fauna and flora, 123 genetic engineering companies, 63
fungal diseases, 123 Herculex corn variety, 63
fungi (see Entomopathogenic fungi) insect-resistant tomato line, 63
herbivores action, 123 lepidopteran insects control, 65
IPM strategies, 123 modification, sequences of genes, 63
microbial control, 123 Monsanto’s Bollgard II cotton, 64
Spodoptera spp (see Spodoptera spp.) rice, 66
synthetic origin, insecticides, 123 tomato and tobacco, cry1A, 61–62
Spodoptera spp.
control measures
B. thuringiensis, 139 U
chemical insecticidal, 137 UCDNC. See University of California Davis
entomopathogenic nematodes, 139 Nematode Collection (UCDNC)
plant products/biopesticides, 138 University of California Davis Nematode
polyhedrosis virus, 139 Collection (UCDNC), 333
344 Index
V hemolymph ingestion, 99
Vegetative insecticidal proteins (VIP), 53 insect’s defense systems, 100
Verticilide life cycle, 99
analogues, 192, 194 liquid cultures, 100
structure, 192, 193 nematode-bacterial association, 99, 100
VIP. See Vegetative insecticidal proteins (VIP) pathogenicity island (PAI), 100
Viruses protein
baculoviruses, 3, 4 GroEL, 102
ODVs, 3–4 insecticidal pilin, 101
rod-shaped enveloped virion, 3 species, 99
toxin
complexes (Tc), 100–101
W complex genes, 100
World Data Centre for Microorganisms Txp40, 101
(WDCM), 330 Xax A and Xax B, 101
World Federation for Culture Collections X. nematophila infection, 100
(WFCC), 330 Xenorhabdus alpha-xenorhabdolysin (Xax)
toxin, 101
X
Xenorhabdus Y
gram-negative bacteria, 99 Yersinia spp., 104–105
hemocytes, 100

10.1007 - 978 3 319 14499 3

Uploaded by

Copyright:

Available Formats

10.1007 - 978 3 319 14499 3

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

10.1007 - 978 3 319 14499 3

Uploaded by

Copyright:

Available Formats

What is the book about?

What is the book about?

What are some of the major insect pests discussed in the book?

What are some of the major insect pests discussed in the book?

Soil Biology

ISSN 1613-3382 ISSN 2196-4831 (electronic)

Library of Congress Control Number: 2015933012

Springer Cham Heidelberg New York Dordrecht London

Printed on acid-free paper

Springer International Publishing AG Switzerland is part of Springer Science+Business Media

February 2015 M.V. Rajam

Lepidopteran pest infestation of economically important plants is a menace to

We are thankful to Dr. Ashok K. Chauhan, Founder President of the Ritnand

Noida, India K. Sowjanya Sree

1 An Introduction to Entomopathogenic Microorganisms . . . . . . . . . 1

10 Beauveria bassiana: Biocontrol Beyond Lepidopteran Pests . . . . . . 219

K. Sowjanya Sree and Ajit Varma

The quality and quantity of the agricultural produce is influenced by a variety of

K.S. Sree (*) • A. Varma

© Springer International Publishing Switzerland 2015 1

1.2 Biological Control

1.3 Entomopathogenic Soil Microorganisms

1.3.1 Entomopathogenic Viruses

1.3.2 Entomopathogenic Bacteria

1.3.3 Entomopathogenic Fungi

1.3.4 Entomopathogenic Nematodes

Entomopathogenic nematodes are obligate parasites of insect species and are

nematodes sprayed on the foliage (Begley 1990). More elaborate details on

1.4 Conclusions and Future Prospects

The insect order Lepidoptera comprises of a large number of economically impor-

Anand R, Tiwary BN (2009) Pathogenicity of entomopathogenic fungi to eggs and larvae of

Caballero P, Aldebis HK, Vargas-Osuna E, Santiago-Alvarez C (1992) Epizootics caused by a

G.V.R. Rao (*) • Ch.S. Kumar • K. Sireesha

© Springer International Publishing Switzerland 2015 11

insecticides, the application of “bio-pesticides” as an eco-friendly measure for pest

2.2 Classification of Baculoviruses

Baculoviruses are occluded, double-stranded DNA (dsDNA) viruses and charac-

Fig. 2.1 Morphological characteristics of nucleopolyhedroviruses (NPVs) and granuloviruses

membranous envelope. Two morphologically distinct but genetically identical viral

environment (Bergold 1982). The infectious, rod-shaped virions are randomly

2.3 Examples of Some Commercial Baculovirus-Based

Over 20 species of baculoviruses have been developed or registered as commer-

2.4 Isolation and Characterization

2.4.1 Morphological Characterization

During natural epizootic conditions, baculoviruses were isolated from larvae of

Fig. 2.4 NPV-infected larvae of H. armigera and A. albistriga: NPV-infected larvae of

2.4.2 Biological Characterization

Biological assessment studies of the MNPVs isolated at ICRISAT farms from

2.4.3 Comparative Analysis of Viral Proteins of Different

2.4.4 Efficacy of NPV Under Different Storage Conditions

Treatment Storage Interaction

2.4.5 Effect of Chemicals on the Shelf Life of NPV

Information on screening of different chemicals or disinfectants on the shelf life of

Treatment Storage Interaction

Table 2.5 Effect of chemicals on the bacterial contaminants in storage

2.4.6 Molecular Characterization

Molecular-level identification, characterization, and evaluation of phylogenetic

2.5 Production Technologies

Historically, several entomopathogenic viruses have been produced in susceptible