Scientific Method

Unit Objectives:
1. Describe the levels of biological organization.
2. Distinguish between inductive and deductive reasoning.
3. Design an experiment to test a given hypothesis using the scientific method.
4. Distinguish among: hypothesis, theory, independent variable, dependent variable, variable to be held constant,
control group, experimental group, and controlled experiment.
5. Describe the scientific method model of learning and distinguish it from experimentation.

Biochemistry & Macromolecules & Enzymes

1. List and discuss the elements that have special relevance to biological systems.
2. Distinguish among and describe the characteristics of the various types of chemical bonds.
3. Explain the significance of weak bonds to biology.
4. Describe how water contributes to the fitness of the environment to support life.
5. Describe a water molecule and explain what properties emerge as a result of this structure.
6. Explain the relationship between the polar nature of water and its ability to form hydrogen bonds.
7. Explain the basis for the pH scale.
8. Compare and contrast acids, bases and buffers, and discuss their significance to living things.
9. Use the pH scale in describing the hydrogen ion concentration in living systems.
10. Describe how carbon skeletons may vary, and explain how this variation contributes to the diversity and complexity of
organic molecules.
11. Discuss the significance of isomers to biology.
12. Identify the major functional groups present in organic compounds.
13. Compare and contrast the major groups of organic compounds — carbohydrates, proteins, lipids, and nucleic acids in
terms of structure and function.
14. Describe how covalent linkages are formed and broken in organic polymers.
15. Distinguish among monosaccharides, disaccharides, and polysaccharides, and discuss those of major importance in
living things.
16. Distinguish among neutral fats, phospholipids, and steroids, in terms of composition, characteristics, and biological
function.
17. Discuss the functions and chemical structures associated with proteins.
18. Describe the structure of nucleotides and nucleic acids, and discuss their importance.
19. Relate the molecular structure to function for each type of biologically important macromolecule.
20. Use chemical and physical tests to identify mono-, di-, and polysaccharides, neutral fats, proteins, and individual
amino acids
21. State the laws of thermodynamics, give examples of each, and apply the first and second laws to organisms and the
ecosphere.
22. Distinguish between exergonic and endergonic reactions.
23. Relate the chemical structure of ATP to its role in cellular metabolism.
24. Summarize the role of enzymes, describe how they work, and discuss factors that effect their action.
25. Use a chemical assay techniques to determine the rates of enzyme catalyzed reactions.
26. Use appropriate methods to design and implement experiments relating to enzyme function.

Practice Questions:

1. Which of the following functional groups d. KBr

characterizes RNA? e. C6H12O6
a. A PO3 group, deoxyribose, and uracil
b. A PO3 group, ribose, and uracil 3. Which of the following compounds would NOT
c. A PO3 group, ribose, and thymine be water-soluble?
d. A PO2 group, deoxyribose, and uracil a. Potassium chloride
e. A PO2 group, deoxyribose, and thymine b. Fatty acids
c. Fructose
2. Which of the following molecules would contain d. Cellulose
a polar covalent bond? e. Hydrogen bromide
a. Cl2
b. NaCl
c. H2O
 4. Three terms associated with the travel of water c. The second law of thermodynamics
from the roots up through the vascular tissues of d. Evolutionary theory
plants are e. The law of increased chaos
a. Adhesion, cohesion and translocation
b. Adhesion, cohesion, and transcription 13. Catalysts speed up chemical reactions by
c. Cohesion, hybridization, and a. Decreasing the free energy change of
transpiration the reaction
d. Cohesion, adhesion, and transpiration b. Increasing the free energy change of the
e. Transpiration, neutralization, and reaction
adhesion c. Degrading the competitive inhibitors in a
reaction
Questions 5-9: Match the appropriate term or concept d. Lowering the activation energy of the
with the phrase that best describes it. reaction
a. lipids e. Raising the activation energy of the
b. peptide bonds reaction
c. alpha helix
d. unsaturated fatty acid Questions 14-18: Match the appropriate term or concept
e. cellulose with the phrase that best describes it.
a. Allosteric inhibition
5. Contain one or more double bonds b. Feedback inhibition
6. one of the four major classes of biological c. Competitive inhibitor
molecules that are not polymers d. Noncompetitive inhibitor
7. linkages between the monomers of proteins e. Cooperativitity
8. a secondary structure of proteins
9. the storage form of carbohydrates in plants 14. Describes inhibition by an enzyme that is
capable of either activating or inhibiting a
10. The process by which protein conformation is metabolic pathway
lost or broken down is 15. A reversible inhibitor that looks similar to the
a. Decondensation normal substrate and competes for the active
b. Deconstruction site of the enzyme
c. Denaturation 16. The process by which the binding of the
d. Hydrolysis substrate to the enzyme triggers a favorable
e. Hybridization conformation change, which causes a similar
change in all of the proteins’ subunits
11. The above graph most accurately depicts the 17. The process by which a metabolic pathway is
energy changes that take place in which of the shut off by the product it produces
following types of reactions? 18. Binds to the enzyme at a site other than the
a. Hypothermic active site, causing the enzyme to change shape
b. Hyperthermic and be unable to bind to the substrate
c. Endergonic
d. Exergonic 19. A + B  AB + Energy Which of the following
e. Free range best characterizes the reaction represented
above?
a. Metabolism
b. Anabolism
c. Catabolism
d. Endergonic reaction
e. Exergonic reaction
20. When a water molecule loses an H+ in solution,
and another water molecules accepts the
proton, what is the water molecules that accepts
the proton called?
a. A hydroxide ion
12. Which of the following theories or laws states b. Crystal lattice structure
that every energy transfer increases the amount c. A solution not in equilibrium
of entropy in the universe? d. A hydronium ion
a. The free energy law e. A basic solution
b. The first law of thermodynamics

Sample Essay Questions:

1. Phospholipids are a critical component of the cell wall. The cell wall is selectively permeable, allowing only
certain substances in and out, and in certain amounts.
 a. Describe why you think phospholipids are good candidates to form the cell wall, based on their structure
and properties.
b. Explain why proteins are an important component of the cell wall, based on their structure and properties.

2. Proteins functioning as enzymes exhibit precise specifications.

Discuss the levels of structural organization within proteins which are responsible for
specific molecular interaction.

3. A class of biology students performed an experiment on the digestion of starch by salivary

amylase. Each student determined the length of time required for different dilutions of his saliva
to digest completely a standard concentration of starch. Iodine was used to test for the presence
of starch. The results obtained by some of the class are summarized in the table below.

TIME REQUIRED FOR THE DISAPPEARANCE OF STARCH WITH VARIOUS

SALIVA DILUTIONS
Dilutions (saliva: H2O)

Student 1:9 (10%) 1:19 (5%) 1:49 (2%) 1:99 (1%)

A 45 seconds 50 seconds 100 seconds 135 seconds
B (no end point) ----------------------------
C 90 seconds 100 seconds 200 seconds 270 seconds
D 260 seconds 300 seconds 600 seconds 800 seconds

a. Present the data for Student A in graphic form.

b. Carefully examine the data collected by the four students above and state
as many conclusions as you can that are supported by these data.
c. Assuming there have been no errors in techniques, form as many hypotheses
as you can to explain the differences observed.
d. Design one experiment to test the validity of one hypothesis.

Clearly state what data you would want to collect in this experiment to test your
hypothesis.

4. Discuss the biological importance of each of the following organic compounds in relation to
cellular structure and function in plants and animals.
a. Carbohydrates
b. Proteins
c. Lipids
d. Nucleic acids

5. The unique properties (characteristics) of water make life possible on Earth. Select three
properties of water and:

a) for each property, identify and define the property and explain it in terms of the
physical/chemical nature of water.

b) for each property, describe one example of how the property affects the functioning of
living organisms.
 Cells & Cell Transport
1. Justify why the cell is considered the basic unit of life.
2. Point out the basic needs of all living things and explain how a cell is able to meet its needs.
3. Differentiate between pro- and eukaryotic cells.
4. Evaluate the size relationships among different cells and cell structures.
5. Explain why there are both upper and lower limits to cell size.
6. Locate and describe the structures and relate the functions of principal organelles in plant and animal cells.
7. Describe the structure of the nucleus and relate this information to its function in the cell.
8. List the components of the endomembrane system, describe its structure and functions, and summarize the
relationships among these components.
9. Follow the fate of a protein synthesized on the endoplasmic reticulum as it is processed, modified, and sorted by the
Golgi complex.
10. Describe the functions of lysosomes and explain what happens when they leak.
11. Distinguish between chloroplasts and mitochondria in terms of structure and function and explain why both synthesize
ATP.
12. Describe the structures of the major types of fibers that make up the cytoskeleton. Explain the importance of the
cytoskeleton to the cell.
13. Describe the structure of intercellular junctions found in plant and animal cells and relate their structure to function.
14. Make a detailed sketch of the fluid mosaic model of cell membrane structure.
15. Relate the properties of the lipid bilayer to the physical properties of the cell membrane.
16. Explain how the various classes of membrane proteins associate with the lipid bilayer and discuss the different roles
that membrane proteins assume.
17. Solve simple problems involving osmosis and water potential.
18. Summarize the main ways that small hydrophilic molecules move across the membrane.
19. Differentiate between the processes of facilitated diffusion and active transport.
20. Compare and contrast desmosomes, tight junctions, gap junctions, and plasmodesmata.
21. Contrast the physical processes of diffusion and osmosis with the carrier-mediated physiological processes by which
materials are transported across cell membranes.

Practice Questions:
Multiple Choice:

1. A student looking through a light microscope would

not be able to see any of the following cell structures
EXCEPT
a. ribosomes
b. a Golgi apparatus
c. a nucleus
d. an endoplasmic reticulum
e. a peroxisome
2. Prokaryotic and eukaryotic cells have all of the Questions 3-7: Match the appropriate term or concept
following structures in common EXCEPT with the phrase that best describes it.
a. plasma membrane a. peroxisome
b. protein-complexed DNA b. Golgi apparatus
c. nucleoid region c. Lysosomes
d. cilia d. Endoplasmic reticulum
e. cytoplasm e. Mitochondria

3. An organelle that is characterized by extensive, folded

membranes, and is often associated with ribosomes.
4. An organelle with a cis and trans face, which acts as sugars). Which of the following accurately describes
the packaging and secreting center of the cell. what will take place next?
5. The sites of cell respiration. a. Glucose will diffuse from side A to side B.
6. Single-membrane structures I the cell that perform b. Sucrose will diffuse from side B to side A.
many metabolic functions and produce hydrogen c. No net movement of molecules will occur.
peroxide. d. Glucose will diffuse from side B to side A.
7. Large membrane-bound structures that contain e. There will be a net movement of water from
hydrolytic enzymes and that exist only in animal cells. side B to side A.

8. Which of the following molecules is a typical 10. Which of the following is an example of passive
component of the animal cell membrane? transport across the cell membrane?
a. starch a. The travel of an action potential down a
b. glucose muscle cell.
c. nucleic acid b. The uptake of nutrients by the microvilli of
d. carbohydrates cells lining the stomach.
e. Vitamin K c. The movement of insulin across the cell
membrane.
d. The movement of carbon dioxide across the
cell membrane.
e. The selective uptake of hormones across the
cell membrane.

Figure B 11. The figure B to the left illustrates the process of

a. cotransport
b. passive diffusion
c. receptor-mediated endocytosis
Figure A d. phagocytosis
e. pinocytosis

12. Large molecules are moved out of the cell by which

of the following processes?
a. pinocytosis
b. phagocytosis
9. The drawing, Figure A, above shows two solutions of c. receptor-mediated endocytosis
glucose and sucrose in a U-tube containing a semi- d. cytokinesis
permeable membrane (which allows the passage of e. exocytosis

Essay Questions:

1. Prokaryotic and eukaryotic cells are physiologically different in many ways, but both represent functional collections of
living matter.
a. It has been theorized that the organelles of eukaryotic cells evolved from prokaryotes living symbiotically inside
them. Compare and contrast the structure of the prokaryotic cell with eukaryotic cell organelles, and make an
argument for or against this theory.
b. Trace the path of a protein in a eukaryotic cell form its creation to its excretion as waste from the cell.
2. Describe the fluid-mosaic model of a plasma membrane. Discuss the role of the membrane in
the movement of materials through by each of the following processes.
a. Active Transport
b. Passive Transport
3. Describe the structure of a generalized eukaryotic plant cell. Indicate the ways in which a
nonphotosynthetic prokaryotic cell would differ in structure from this generalized eukaryotic plant
cell.

4. Discuss the process of cell division in animals. Include a description of mitosis and cytokinesis,
and of the other phases of the cell cycle. Do not include meiosis.
 Cellular Respiration & Photosynthesis
1. Write a general equation illustrating hydrogen and electron transfer from a substrate to a hydrogen acceptor such as
NAD. +
2. Write a summary reaction for cellular respiration, giving the origin and fate of each substance.
3. Give an overview of the four phases of cellular respiration, and indicate where the reactions of each phase take place in
the cell.
4. Summarize the events of glycolysis, the citric acid cycle, and the electron transport system.
5. Describe chemiosmotic phosphorylation.
6. Indicate how the products of protein and fat metabolism feed into the same metabolic pathways that oxidize glucose.
7. Contrast aerobic and anaerobic pathways used to extract energy from nutrients in terms of ATP, final hydrogen
acceptor, and end products.
8. Compare alcoholic and lactic acid fermentation in terms of final hydrogen acceptor and end products.
9. Trace the energy flow from the through the steps of the light and dark reactions, ending with sugar.
10. Describe the structure and function of various tissues of a leaf.
11. Correlate the various reactions of photosynthesis with the physical location in the chloroplast where each reaction
takes place.
12. Differentiate between C3, C4, and CAM reactions.

Practice Problems:
Multiple Choice:

1. The purpose of cell respiration in a eukaryotic cell is to b. 2

a. synthesize carbohydrates from CO2 c. 3
b. synthesize fats and proteins from CO2 d. 4
c. break down carbohydrates to provide energy e. 5
to provide energy for the cell in the form of ATP
d. break down carbohydrates to provide energy 5. The process that produces the largest amount of ATP
for the cell in the form of ADP during respiration is
e. provide oxygen to the cell. a. glycolysis
b. fermentation
2. 2K + Br2  2K+ + 2 Br- In the course of the above c. the Krebs cycle
reaction, potassium is d. the electron transport chain
a. neutralized e. chemiosmosis and oxidative phosphorylation
b. oxidized
c. reduced
d. subliminated Questions 6-10
e. recycled a. Chemiosmosis
b. Electron transport chain
3. The net result of glycolysis is c. The Krebs cycle
a. 4 ATP and 4 NADH d. Glycolysis
b. 4 ATP and 2 NADH e. Fermentation
c. 2 ATP and 4 NADH
d. 2 ATP and 2 NADH 1. The process by which glucose is split into
e. 4 ATP and 8 NADH pyruvate
2. The process by which a hydrogen gradient is
4. In the course of the Krebs cycle, how many molecules used to create ATP
of ATP are produced? 3. The process that makes a small amount of ATP
a. 1 and can produce lactic acid as a by product
 4. A series of membrane embedded electron d. do not require light in order to proceed
carriers that ultimately create the hydrogen ion e. take place only during the day
gradient to drive the synthesis of ATP
5. The process by which the breakdown of glucose 15. The major product of the Calvin cycle is
is completed and CO2 is produced a. rubisco
b. oxaloacetate
11. Muscle fatigue is caused when the process of c. ribulose bisphophate
fermentation in oxygen-depleted cells produces which of d. pryruvate
the following? e. glyceraldehydes-3-phaosphate
a. ADP
b. Ethanol
c. Lactate
d. Uric acid
e. Pyruvate 16. All of the following statements are false EXCEPT
a. C3 plants grow better under hot, arid
12. Groups of photosynthetic pigment molecules situated conditions than do C4 plants
in the thylakoid membrane are called: b. C4 plants grow better under cold, moist
a. photosystems conditions than do C3 plants
b. carotenoids c. C3 plants grow better under hot, arid
c. chlorophyll conditions than do CAM plants
d. granna d. CAM plants grow better under cold, moist
e. CAM plants conditions than do C3 plants
e. CAM plants and C4 plants both grow better
under hot, arid conditions than do C3 plants

17. All of the following statements about photosynthesis

13. The process in photosynthesis that bears the most are true EXCEPT
resemblance to chemiosmosis and oxidative a. the light reactions convert solar energy to
phosphorylation in cell respiration is called chemical energy in the form of ATP and NADPH
a. photolysis b. the Calvin cycle uses ATP and NADPH to
b. noncyclic photophosphorylation convert CO2 to sugar
c. ATP synthase coupling c. photosystem I contains P700 chlorophyll a
d. preemptive photphosphorylation molecules at the reaction center; photosystem II
e. dark reaction nphsophorylation contains P680 molecules.
d. in chemiosmosis, electron transport chains
14. The reactions of the Calvin cycle are also known as pump protons (H+) across a membrane from a
the dark reactions because these reactions region of high H+ concentration to a region of
a. occur in plants only at night low H+ concentration
b. occur in dark-staining cells of plant leaves e. the steps of the Calvin cycle are sometimes
c. must absorb black light from the spectrum in referred to as the dark reactions, because they
order to proceed do not require light in order to take place

Essay Questions:
1. Compare the intermediate steps in the fermentation of a molecule of sugar by yeast with
respiration in a muscle tissue cell. Include in your answer the role of ATP formed in each of these
two processes.

2. Explain how the molecular reactions of cellular respiration transform the chemical bond energy
of Krebs cycle substrates into the more readily available bond energy of ATP. Include in your
discussion the structure of the mitochondrion and show how it is important to the reactions of the
Krebs cycle and the electron transport chain.

3. Explain what occurs during the Krebs (citric acid) cycle and electron transport by describing
the following:
a. The location of the Krebs cycle and electron transport chain in the mitochondria.
b. The cyclic nature of the reactions in the Krebs cycle.
c. The production of ATP and reduced coenzymes during the cycle.
d. The chemiosmotic production of ATP during electron transport.

4. The results below are measurements of cumulative oxygen consumption by germinating and dry
seeds. Gas volume measurements were corrected for changes in temperature and pressure.

Cumulative Oxygen Consumed (mL)

______________________________________________
Time (minutes) 0 10 20 30 40
______________________________________________
22o C Germinating Seeds 0.0 8.8 16.0 23.7 32.0
Dry Seeds 0.0 0.2 0.1 0.0 0.1
______________________________________________
10o C Germinating Seeds 0.0 2.9 6.2 9.4 12.5
Dry Seeds 0.0 0.0 0.2 0.1 0.2
______________________________________________

a. Using the graph paper provided, plot the results for the germinating seeds at 22 o C and
0o C.
b. Calculate the rate of oxygen consumption for the germinating seeds at 22 o C, using the
time interval between 10 and 20 minutes.
c. Account for the differences in oxygen consumption observed between:
1) germinating seeds at 22o C and at 10o C;
2) germinating seeds and dry seeds.
d. Describe the essential features of an experimental apparatus that could be used to
measure oxygen consumption by a small organism. Explain why each of these features is
necessary.

5. The process of photosynthesis consists of two phases, the light reactions and the dark
reactions. Discuss each of these groups of reactions and their interrelationships.

6. Describe the light reactions of photosynthesis and, for both a C 3 and a C4 plant, trace the path
of a carbon dioxide molecule from the point at which it enters a plant to its incorporation into a
glucose molecule. Include leaf anatomy and biochemical pathways in your discussion of each type
of plant.

Cell Cycle, Mitosis & Meiosis, Genetics

1. Illustrate the structure of a duplicated chromosome, labeling sister chromatids, sister centromeres, and sister
kinetocores.
2. Identify the stages of the eukaryotic cell cycle; describe the principal events of each stage and point out ways in which
the cycle is controlled.
3. Diagram the process of mitosis.
4. Contrast the process of cell division in animal and plant cells.
5. Compare and contrast mitosis and meiosis in terms of events and results.
6. Use correctly the terms haploid, diploid, homologous chromosomes, allele, tetrad, synapsis, locus, genotype,
phenotype, dominant, recessive, homozygous, heterozygous, and test cross.
7. Apply Mendel's principles to solve problems involving the genetics of one and/or two traits at a time.
8. Discriminate between independent and mutually exclusive events; apply the product rule and sum rule appropriately in
predicting outcomes.
9. Solve problems involving incomplete dominance, epistasis, polygenes, multiple alleles, & X-linkage.
10. Explain some of the ways that genes interact to affect the appearance of a single trait.
11. Analyze data from a test cross to distinguish between independent assortment and linkage.
12. Relate segregation and independent assortment to specific events in meiosis.
13. Discuss the genetic determination of sex and the role of the Y chromosome in humans. Contrast the method of sex
determination in mammals with the method used in other animals and plants.
14. Explain why humans have been traditionally considered poor subjects for inheritance studies.
15. Distinguish between environmentally induced and inherited abnormalities.
16. Make and use human karyotypes to assess the genetic make-up of an individual.
17. Make a sketch showing how non-disjunction can occur during meiosis for Down's, Turner, and Klinefelter’s syndromes.
18. Relate the ABO blood typing system to the appropriate genotypes. Explain the genetic and physiological basis for Rh
incompatibility between mother and infant.

Practice Problems:

Multiple Choice:

Questions 1-5 d. ¼ + ¼
a. Telophase e. ½ + ½
b. Interphase
c. Cytokinesis 10. In llamas, coat color is controlled by a gene that
d. prometaphase exists in two allelic forms. If a homozygous yellow llama
e. anaphase si crossed with a homozygous brown llama, the offspring
have gray coats. If two of the gray-coated offspring were
1. Cytokinesis begins during the final stage of mitosis crossed, what percentage of their offspring would have
2. Division of the cytoplasm of the cell. brown coats?
3. Sister chromatids begin to separate a. 100%
4. The genetic material of the cell replicates to prepare b. 75%
for cell division c. 50%
5. Microtubules begin to attach to the centromeres of the d. 25%
sister chrmatids e. 0%

11. All of the following are true of the process of mitosis

EXCEPT
a. during prometaphse, spindle microtubles come into
contact with chromosomes
6. The figure at above shows a cell in what stage of b. the chromosome number in the newly formed cells is
mitosis? half that of the parent cell
a. prophase c. the chromosomes line up along the metaphase plate,
b. prometaphase or equator of the cell
c. metaphase d. the cytoplasm of the cell and all its organelles are
d. anaphase divided approximately in half
e. telophase e. in anaphase, the sister chromatids travel to opposite
ends of the cell
7. After which of the following checkpoints in the cell
cycle is the cell fated to divide?
a. G2 phase checkpoint
b. M phase checkpoint
c. Interphase checkpoint
d. G1 phase checkpoint
e. MPF checkpoint

8. A couple has 6 children, all daughters. If the woman 12. In rabbits, the trait for short hair (S) is dominant, and
has a seventh child, what is the probability that the the trait for long hair (s) is recessive. The trait for gree
seventh child will be a daughter? eyes is dominant (G) and the trait for blue eyes is
a. 6/7 recessive (g). A cross between two rabbits produces a
b. 1/7 litter of 6 short-haired rabbits with green eyes, and 2
c. 1/36 short-haired rabbits with blue eyes. What is the most
d. 1/49 likely genotype of the parent rabbits in this cross?
e. ½ a. ssgg X ssgg
b. SSGG X SSGG
9. If alleles R and S are linked, and (considering the c. SsGg X SsGg
effects of crossing over) the probability of gamete R d. SsGg X SSGg
segregating into a gamete is ¼, while the probability of e. ssGG x ssGG
allele S segregating into a gamete is ½, what is the
probability that both will segregate into the same 13. In most organisms, reproduction occurs sexually; two
gamete? parents are involved and genetic recombination occurs.
a. ¼ X ½ However, some organisms reproduce asexually, and
b. ¼ ÷ ½ only one parent contributes genetic material. Which of
c. ¼ + ½ the following organisms reproduce asexually?
a. Annelids e. O only
b. Arthropods 18. If the type O individual were to mate with a person
c. Hydra with type AB blood, which of the following is the best
d. Angiosperms calculation of the ratio and genotype of the offspring?
e. gymnosperms a. 3:1, IAi: IBi
b. 2:1, IAi: IBi
14. In humans, hemophilia is a sex-linked recessive trait. c. 1:1, Iai: IBi
If a man and a woman produce a son that is affected d. 1:2:1, IAi: IAIB: IBi
with hemophilia, which of the following is definitely true? e. 9:3:3:1, IAIB:IAi:IBi:O
a. the mother carries an allele for hemophilia 19. Two yellow mice with the genotype YyX Yy are
b. the father carries an allele for hemophilia mated. Two-thirds of their offspring are yellow, and 1/3
c. the father is affected with hemophilia of their offspring are not yellow. Mendelian genetics
d. neither parent carries an allele for hemophilia dictates that this cross should produce offspring that
e. the boy’s paternal grandfather is hemophiliac were ¼ YY, ½ Yy, and ¼ yy. What is the most likely
conclusion from this experiment?
15. Which of the following explains a significantly low a. The mice did not bear enough offspring for the ratio
rate of crossing over between two genes? calculation to be specific.
a. They are located far apart on the same chromosome b. Y is a lethal allele and caused death early in
b. They are located on separate but homologous development.
chromosomes c. Nondisjunction occurred.
c. The genes code for proteins that have similar d. A mutation masked the effects of the Y allele.
functions e. A mutation masked the effects of the y allele.
d. The genes code for proteins that have very different
functions
e. The genes are located very close together on the 20. In organisms that undergo alternation of generations,
same chromosome. the diploid stage is called the
a. gametophyte
b. ovum
c. sporophyte
d. hybrid
e. dicot

21. All of the following contribute to genetic

recombination EXCEPT
a. random fertilization
b. independent assortment
c. crossing over
16. In the pedigree above, which inheritance best d. gene linkage
describes the pedigree for this trait? e. random gene mutation
a. sex-linked recessive
b. sex-linked dominant 22. In cucumbers, warty (W) is dominant over dull (w),
c. autosomal recessive and green (G) is dominant over orange (g). A cucumber
d. autosomal dominant plant that is homozygous for wateriness and green color
e. codominant is crossed with one that is homozygous for dullness and
orange color. The F1 are then crossed to produce an F2
Questions 17-18 refer to an individual with blood type O, generation. If a total of 144 offspring are produced,
whose mother has blood type A. which of the following is the closest to the number of dull
17. The father must have which of the following blood green cucumbers expected?
types? a. 3
a. A, B, or O b. 10
b. AB or A c. 28
c. AB or B d. 80
d. AB only e. 161

Practice Essay Questions:

1. Genes are located on chromosomes and are the basic unit of heredity that are passed on from parent to child, through
generations.
a. Explain how a chromosome mutation could occur, and why mutations are detrimental to the organism in which
they take place.
b. Explain why it is that human males suffer from having just one copy of the X chromosome, while females have
two.
2. a. In corn, a gene for colored (C) kernels is dominant over one for colorless (c) kernels and a
gene for smooth (S) kernels is dominant over one for shrunken (s) kernels. Describe a controlled
genetic experiment to demonstrate that those genes are linked.
b. Genetic evidence indicates that these genes are linked and that their cross value is
approximately 4%. Describe the process of crossing over and explain how its percentage is
determined. (Labeled diagrams may be used as aids in explanation.)

3. Discuss three of the following phenomena in which sex chromosomes are involved with
particular reference to their significance or consequences in humans.
a. Sex determination
b. Sex-linked inheritance
c. Formation of Barr bodies (sex chromatin)
d. Variation in kinds and numbers of sex chromosomes

4. Discuss Mendel's laws of segregation and independent assortment. Explain how the events of
meiosis I account for the observations that led Mendel to formulate these laws.

Molecular Genetics
1. explain why protein was originally thought of as the genetic material.
2. summarize the experiments and conclusions of Griffith;, Hershey & Chase; and Chargaff.
3. describe the structure of a nucleotide and how nucleotides form a DNA molecules.
4. explain how Watson and Crick deduced the structure of DNA, and describe the evidence they used.
5. distinguish between conservative and semi-conservative replication of DNA and summarize the experiment used to
resolve this question.
6. summarize the process of DNA replication.
7. define anti-parallel, and explain why continuous synthesis of both DNA strands is not possible.
8. explain the role of DNA polymerase, ligase, and repair enzymes.
9. compare and contrast the processes of transcription and replication.
10. identify the features of tRNA that are important in decoding genetic information and translating it into protein
"language"
11. diagram the process of initiation, chain elongation, and chain termination in the translation phase of protein synthesis.
12. give examples of the different classes of mutations that affect the base sequence of
DNA and demonstrate the effects each has on the protein produced.
13. explain why the organization of genes into operons is advantageous to bacteria.
14. diagram the main components of an inducible operon and explain the functions of the operator and promoter regions.
15. differentiate between positive and negative control.
16. compare pro- & eukaryotic regulatory systems.

Practice Problems:

Multiple Choice:

1. Which of the following exists as DNA surrounded by a spiders when particular genes from a spider are inserted
protein coat? into the goat’s genome. Which of the following reasons
a. retrovirus describes why this is possible?
b. virus a. Goats and spiders share a common ancestor and thus
c. eukaryote produce similar protein excretions.
d. prokaryote b. The opposite is true, too—when genes from a goat
e. ampicillin are inserted into a spider’s genome, the spider produces
goats’ milk instead of silk.
2. A goat can be made to produce milk containing the c. The proteins in goats’ milk and spiders’ silk have the
same polymers present in the silk produced by the same amino acid sequence.
d. The processes of transcription and translation in the 19. All of the following nitrogenous bases are included in
cells of spiders and goats are fundamentally similar. DNA EXCEPT
e. The process of transcription and translation in the a. adenine
cells of spiders and goats produce exactly the same b. cytosine
proteins anyway. c. guanine
d. thymine
e. uracil

20. The actions of which of the following enzymes are

responsible for ensuring that chromosomes do not
decrease in length with every round of replication?
a. Telomerase
b. DNA ligase
Questions 3-7: c. DNA polymerase
a. transcription d. Helicase
b. translation e. Primase
c. transposon
d. DNA methylation
e. Histone acetylation

3. A mobile segment of DNA that travels from one

location on a chromosome to another, one element of
genetic change.
4. The addition of methyl groups to certain bases of DNA
after DNA synthesis, this is thought to be an important
control mechanism for gene expression.
5. The synthesis of polypeptides from the genetic
information coded in mRNA.
6. The synthesis of RNA from a DNA template
7. The attachment of acetyl groups to particular amino
acids of histone proteins, this is thought to be an
important control mechanism for gene expression.

21. After eukaryotic transcription takes place, mRNA

undergoes several modifications before leaving the
nucleus to take part in translation. One of these is the
cutting out of nonessential sections of mRNA, and the
subsequent splicing together of stretches of mRNA
necessary for the final functional molecule. Which of the
following sections of mRNA are spliced together into the
finished mRNA molecule?
a. introns
b. exons
c. genes
d. coding sequences
Questions 14-18 e. ribozymes
a. tRNA
b. mRNA 23. At the end of DNA replication, each of the daughter
c. poly-A tail molecules has one old strand, derived from the parent
d. RNA polymerase strand of DNA, and one strand that is newly synthesized.
e. rRNA This explains why DNA replication is described as
a. conservative
14. An example of a post-transcriptional modification b. largely conservative
15. Binds to the promoter on DNA to initiate transcription c. nonconservative
16. Along with proteins, comprise ribosomes d. semiconservative
17. Loosely binds to free amino acids in the cytoplasm e. unconservative
18. Travels out of the nucleus and into the cytoplasm to
participate in translation 24. The expression of genes can be controlled at all of
the following stages EXCEPT
a. initiation of transcription
b. RNA processing d. degradation of protein
c. DNA unpacking e. protein folding

Essay Questions:
1. Deoxyribonucleic acid or DNA has been described as the chemical basis of heredity. Discuss
present-day concepts regarding its:
a. chemical nature and physical structure
b. mode of duplication
c. relationship to protein synthesis

2. Proteins are composed of amino acid subunits which form stable three-dimensional structures.
a. Describe how the genetic instructions coded in DNA are translated into the
primary structure (sequence of amino acid subunits) of a protein molecule.
b. Explain how interactions among the individual amino acid subunits influence
the transformation of the molecule into its three-dimensional structure and
how they stabilize it.

3. Experiments by the following scientists provided critical information concerning DNA. Describe
each classical experiment and indicate how it provided evidence for the chemical nature of the
gene.
a. Hershey and Chase - bacteriophage replication
b. Griffith and Avery - bacterial transformation
c. Meselson and Stahl - DNA replication in bacteria