Science Investigatory Final
Science Investigatory Final
Presented to the
SCIENCE DEPARTMENT
BY:
Rodgie S. Avendaño
Gianna P. Gamo
S.I.P. Adviser
February, 2019
Acknowledgement
We, the researchers, would like to express our deepest and utmost gratitude to all the
people who had been involved in the journey of making this project possible; who had given their
full support and provision throughout the process of this investigatory project.
To Ms. Sheillemae Cresse Duey Delim, who had granted the researchers permission to use
the TLE laboratory; allowing the team to make use of the necessary equipment needed, and the
To our science teacher, Mr Russel V. Galera, who had supervised the making of each
chapter of this Science Investigatory Project. It is through him that this investigatory project had
All of the efforts of the people who have been involved are thoroughly appreciated. Once
again, we tip our hats to all the individuals that have supported the researchers all throughout, it is
- The Researchers
Abstract
Bones are organic material proven to be as strong as metal, yet as light as aluminum. The
investigatory project entitled “Bone Tiles as Viable Flooring”, was conducted to test whether the
alternative tile as flooring that will ensure durability. The notable decrease in the availability of
resources and lack of tile flooring in poverty-stricken has driven the researchers to choose this topic
for investigation.
The researchers gathered the necessary materials, and a total of 2 trials were held. The first
trial, with a total of 3 days of observation, direct heat was applied for the drying process by placing
the mixture inside an oven for 15 minutes. The resulting tile was of a clay-like consistency and the
odor was evident. In the second trial, with a total of 4 days of observation, the mixture was allowed
to air-dry, and the resulting tile was a lot more solid, but not enough to be an enduring tile. The
In conclusion, there was fault in the process of cleaning and a lack in the addition of the
binder (cornstarch), which had resulted into a tile that had not met the expectations.
i
TABLE OF CONTENTS
Abstract …………………….. i.
I. Chapter I: Introduction
1.3 Hypothesis 3
3.1 Methodology 12
3.1.1 Materials 12
3.1.2 Procedures 12
4.1 Results 19
5.1 Conclusion 22
5.2 Recommendations 23
Bibliography 24
CHAPTER I:
Introduction
The floors of a house take the brunt of our everyday activities, and are usually the first
surfaces to reflect wear and tear, as such it is important to invest in floors that will keep pace with
our daily life (Gray, 2011). Tiles are considered as a great flooring material in homes since it
requires less maintenance, having a protective layer that rests over the material, making them
impervious to water and stain penetration, unreceptive to dust mites, molds, germs and bacteria.
Tiles are also well-known for its durability, lasting for about 10-20 years if installed properly and
handled with care, which is why people choose to invest in getting tiles as flooring (Lewitin, 2017).
Our study focuses on bones being a good alternative to cement as flooring. We will be devising a
preservation process wherein the bone will be submerged in hydrogen peroxide (H2O2), which will
prevent the bone from decaying. We have chosen bones as the main compliant because it is a truly
incredible substance, it is as strong as steel but at as light as aluminum. Not only is it strong, but
also surprisingly flexible as well. Its components are calcium phosphate and collagen, which are
both extremely weak. But nature mixes them together at room temperature and without toxic
Bones have piqued the interest of the researchers because of its durability and composition
professor in MIT’s Department of Civil and Environmental Engineering (CEE), Markus Buehler, CEE
department head, along with their team are seeking to redesign concrete—the most widely used
human-made material in the world—by following nature's blueprints. As observed by the researches
1
through a comparative study on cement paste—concrete's binding ingredient—with the structure
and properties of natural materials such as bones, shells, and deep-sea sponges. As the researchers
observed, these biological materials are exceptionally strong and durable. Ultimately, the team
hopes to identify materials in nature that may be used as sustainable and longer-lasting alternatives
"If we can replace cement, partially or totally, with some other materials that may be readily
and amply available in nature, we can meet our objectives for sustainability," Buyukozturk says. –
“Researchers look to bones and shells as blueprints for stronger, more durable concrete” (Chu,
2016).
There is an inadequacy of tiles in poverty-stricken places, as they do not have the privilege to
afford installation of tiles due to financial situation. The rapid decrease in the availability of
create a tile that can endure the different weather conditions of the Philippines, throughout
identify what are the elements that make bones so durable, that may help in developing a
finer tile;
create organic tiles, using bones of various animals, like chicken and beef; and
2
1.3 Hypothesis
The firmness and enduring quality of bones makes it an efficient component in creating an
One of the targets is to create organic tiles from bones. The significance of this study for the
researchers is to be able to get more information with regards to organic tile making that can be
taught to those in our community, and can also be utilized as a livelihood project. Alongside this,
we are teaching these communities how to repurpose leftover bones from meals instead of burning
This may also be relevant to the large part of our country struggling with poverty, as well as
the victims of calamities. Instead of buying and using already scarce resources, one can easily
create their own tiles for the creation of houses and such, out of left-over bones. This is also
relevant to the lengthy time it takes for bones to decompose, as they can be recycled and used for
the benefits of thousands in a community and primarily our environment. The manufacturing of
cement tremendously consumes energy, to further solidify this point, during the 8th World Energy
System Conference (WESC) 2010, It stated that about 26% of the total electrical power is used in
grinding raw materials. During the grinding process, the energy obtained from the rotary burner is
consumed, (Adem Atmaca, 2010). So by using organic materials such as bones in making a type of
tile as an alternative to cement, we are promoting the conservation of energy wherein our
3
This study may also benefit the researchers in such ways as it being a plausible reference
for future experimentations, it being a good vantage point for others when having further studies
with regards of creating such tiles that may either be a good or finer alternative rather that of
regular tiles.
Our study mainly focuses on how bones can serve as viable alternative for cement as flooring,
despite it being the researchers’ aim is to conduct a study on 6why bones may or may not be a
good variable in manufacturing durable and long lasting tiles. Though bones do decay in due time,
we are devising a structured experimentation to ensure its resilience. This research aims to do all
necessary experimentations within the school premises and in specific time stamps, without
interfering with other subjects’ daily scheduled tasks. For the location, we may develop our tiles in
the science laboratory of our school, and if ever needed, in the high school open area.
We will base our materials on the resources available, and setting a certain budget. For the
bones, we are planning on using ones that are leftover from meals. If there are ever some
materials that we need to buy, we will make sure to keep it as cheap as we can to pertain to one of
4
CHAPTER II: Review of Related Literature
Tiles have been around as early as 700 B.C originally from Egypt; popularized because of it
being aesthetically pleasing to the eyes, and can endure various activities from our everyday lives
(Sydney Myer, 2011). It is widely known that the manufacturing of tiles can consume copious
amounts of energy. That after coal-powered electricity, cement manufacturing is the next biggest
In the creation of completely organic tiles, the variables in itself are completely natural, and
no artificial additives were augmented with it. These variables as mentioned in chapter one are
animal bones, and now added with starch as an organic binding agent.
Bones, they are rigid tissues consisting of cells embedded in an abundant hard intercellular
material. The two principal components of this material are: collagen and calcium phosphate, both
that are naturally weak on their own, but is mixed to become as strong as metal, but as light as
The animal bone to be used in the experimentation is of pigs and cows, for they are both
mammals and have similar bone structures. Though Mammal bones in particular are denser and
thicker than human bones, but they do have similar molecular composition (James Watson, 2016).
When we look closely at bones and muscles, they are similar across species. You’d need a
microscope to see this, but it would show the tiny living cells that make up animals and humans’
bones and muscles; you can say the reason the human structure looks different from an animal is
5
really about the different shapes and sizes of bones between species (John McClelland, 2016).
Bones are generally stronger than concrete, but bone structure is somewhat similar to
reinforced concrete. Protein strands make up the rebar of bone. Calcium and phosphorus mineral
crystals deposited around the protein strands are somewhat like the concrete poured around the
Although bones do decompose in time, research has shown that it is possible to prevent them
from decaying, like how skeletons are preserved in museums. Individuals usually use hydrogen
peroxide, to bleach the bone, but not enough to damage or cause the bone to brittle (Claire Lower,
2017).
Hydrogen peroxide (H2O2), it works by oxidizing compounds that are common in organic life.
It reacts particularly well when in the presence of catalase, an enzyme that occurs naturally in cells,
by releasing water and oxygen. When the water and oxygen are released, it causes the fizzing
process that you associate with hydrogen peroxide. It dissolves proteins by breaking apart and
releasing water and oxygen free radicals. Giving it the potential not only to disinfect but also oxidize
water having strong binding properties; corn starches are no different to these aspects. Chemical
derivatives have properties like esters of cellulose but are not stable against bacteria. Bacteria
usually are the cause of the decomposition of organic binders, so we therefore have to add an anti-
Amylase is the bacterium that breaks down starch molecules; Bacteria and fungi secrete
6
amylases for extracellular digestion. When they have broken down the insoluble starch, the soluble
end products such as (glucose or maltose) are absorbed into their cells (Vipin Kannan, 2011)
agent itself. When two of these constituents are mixed together, they modify the starch into an
oxidized starch, which helps prolong the longevity of the starch (Pasi Tolvane, 2015)
Oxidized starches have shorter chain lengths than native starches. It improves whiteness and
reduces microbiological content. Oxidized starches are the best thickener for applications requiring
gels of low rigidity. This therefore improves adhesion. This type of modified starch is also used in
the production of paper and serves as stabilizers in ice-cream and milk pudding production (Nadiya
Baal, 2017)
An article called: “Clandestine operation: Bones from Carcass Cold Meat to Tiles
and Animal Feed”, written by Nabinder Bommala was released mid 2018, the said article claims
that the usage of bones of dead animals (carcasses), which are dumped at Great Hyderabad
Municipal Corporation (GHMC) dump yard at Autonagar near LB Nagar (India), cam be clandestinely
turned into powder for use in manufacturing tiles and as animal feed. They pound the bones to
Many agencies, which have the technology to turn animal waste into ceramic ash, have
7
approached the civic body, but their bids have been thwarted. The GHMC officials states that; “For
the sake of the environment and people staying near the dump, animal waste should be disposed
of in a scientific manner.”
The main focus of this study is on the recycling of fish bone waste by undergoing a process
of calcination in order to obtain the fish bone ash, which is to be used in the production of
stoneware tiles. The never-ending call for new materials to replace traditional ceramic raw materials
makes use of wastes an important research focus. Any calcium-containing materials are potentially
suitable fluxing additives in the production of ceramic bodies. Fish bone ash resulting from heat
treatment fish waste is composed mainly of hydroxyapatite (HA) phase. This inexpensive material
could be useful as a biologically and environmentally compatible raw material media in the ceramic
industry.
The primary objective was to study the effects of partial replacement of traditional feldspar
by fish bone ash in the production of stoneware tiles and to measure changes in the tiles’
Similar to the objective of our study, an extensive research was conducted, pertaining to the
environmental awareness. The study was monitored by the Los Baños-cased Department of Science
and Technology-Philippine Council for Aquatic and Marine Research and Development (DOST-
PCAMRD). Currently headed by Executive Director Rafael D. Guerrero III, PCAMRD is DOST's
8
planning council for fisheries and aquatic resources that coordinates the national aquatic resources
research and development network (NARRDN) now composed of more than a hundred R&D
Ubando and Nones followed processing steps such as washing and cleaning the shells,
boiling to remove the impurities, air-drying, pounding and pulverizing, molding, drying, and impact
testing.
The tiles produced were left to dry for a time period of 15 to 25 days.
As reported by PCAMRD's Dr. Dolly DG Fernandez, the ground shells mixed with Styrofoam
resulted in grayish tiles similar to the granite tiles, has similar texture, and were stronger and
A piece of commercial tile costs P10 while the shell tile fetches a price of only P4.10 per
Findings of the NVSU study also showed that the shells are sources of calcium oxide (CaO),
determine its endurance to weight or pressure and should be properly glazed, Ubando and Nones
recommended.
Cement Content by Animal Bone powder in Normal Concrete Mix Production” wherein
bone samples accumulated from Seka Waste Disposal Site of Jimma town were the primary object
9
The aim of this research was to analyze the effects of varying dosage replacement of
cement by animal bone powder in the normal concrete strength properties. To achieve the major
to determine the properties of the animal bone powder and bone blended cement at
different percent dosage of replacement and to compare with the standard specifications.
to determine and analyse the workability, compressive strength, flexural strength and tensile
strength of the concrete by partial replacement of cement with animal bone powder at different
to determine the optimum percent dosage of animal bone powder as a partial replacement
Animal bone serves as an essential reservoir for calcium, which can be drawn upon when
required for special metabolic activities. 97% of the total calcium in body accumulates in the
skeleton. On the other hand, anticipated half of the Portland cement weight has calcium oxide
(CaO), the primary raw material in the production of cement. Therefore, this indicates that cement
For that reason, utilization of the bone as a sustainable material in concrete production
would help to preserve natural resources and maintain ecological balance. The waste production of
animal bone in Ethiopia accounted of 10% population of cattle's are slaughtered per year, and the
average weight of cow and ox's 300kg plus out of this mass from 20% to 30% are the weight of
bone, we can get the average of 400.5 million Kgs animal bone generates annually as waste.
A forum online called “Ask MetaFiller: Querying the Hive Mind” comes up with a
detailed analysis about the association of the elements of bones and limestone, a modernized type
10
”Bones are primarily calcium carbonate which is also what some kinds of limestone are
made out of which is what cement is made out of at least modern Portland cement. So if you
processed the bones the same way you process limestone you would likely get a similar product.”
Calcination
It is the process of subjecting a substance to the action of heat, but without fusion, for the
Concrete
material used extensively in buildings, bridges, roads and dams. Its uses range from structural
Extracellular Digestion
It is a form of digestion wherein the breaking down of materials into smaller, absorbable
Mammals
A mammal is any warm-blooded vertebrate having the skin more or less covered with hair.
The young are born alive except for the small subclass of monotremes and nourished with milk.
Tile
A tile is a thin object usually square or rectangular in shape. Tile is a manufactured piece of
hard-wearing material such as ceramic, stone, metal, baked clay, or even glass. Tiles are often used
to form wall and floor coverings, and can range from simple square tiles to complex design.
11
CHAPTER III
3.1 Methodology
Oven
3.1.2 Procedure
1. Begin by cleaning the bones, removing unnecessary tissues and fat, thoroughly wash it with
12
2. Leave the bones to dry for a few hours,
3. Then prepare to soak them in a hydrogen peroxide formula, about 240 ml of hydrogen
peroxide, 50% water and 50% hydrogen peroxide. Leave the bones soaked for at least
another couple of weeks, changing the formula when the water gets cloudy.
13
Figure 4: Leave the bones again
5. Pulverize the bones into a powdered state, you may use a hammer for this.
6. Then begin to create your mixture in your braiser pan, first pour 1 ½ cup of water, then 2
14
Figure 6: Mix all ingredients in the braiser pan
15
Figure 8: Put a few drop of hydrogen peroxide into the mixture
9. Prepare your tile molder, by placing aluminum foil over it; this is so that the tile won’t stick
to your molder.
16
10. Pour your formula into the mold and see to it that the surface is levelled. Leave it to dry for
The central point of observation in the experimentation conducted by the researchers are
the animal bones, which the researchers have chosen to use as the main component of a tile
17
constituting of organic materials locally available; which we have chosen to test and observe in the
creation of an organic tile whether or not it can be an essential component that will guarantee
As it was mentioned in Chapter 1, the study took place within the school premises, namely
the physics and TLE laboratory. The specific time stamp we had taken into consideration was during
dismissal, to not further plague other tasks for certain subjects. The variable was kept at the
average room temperature of 31.0° Celsius (87.8° Fahrenheit), leaving it at the TLE lab to dry, it
18
CHAPTER IV
4.1 Results
The following will consist of further observations and a thorough summary of each trial the
researchers has conducted. And therefore the researchers have decided to use a systematized way
of rating the progress of the durability and odor produced by the tile each day of observation; 10
On the first day of observation of the initial trial, in which the mixture was placed inside an
oven to expose to heat. The output was left to cool atop a table once the heating process was
In the succeeding days, the stench only seemed to worsen and the tile seemed to develop
in terms of solidification, but not sufficient enough; as it could easily be manipulated and molded.
After the second day, there happened to be no change in durability and the mixture remained the
same consistency; but the stench seemed to worsen as more days passed.
19
Table 1: Observation of the odor and durability of the tile from day 1 to 3; for trial 1.
stench)
Trial 2: Airdry
On the first day of the trial prior to the initial one, the mixture was left exposed to air to
allow the air-drying process to take place. The odor of the mixture was not as worse as the first
On the second day, the mixture seemed to further harden, although the stench seemed to
worsen. For the succeeding days, the stench persevered. Although the mixture seemed to harden
further; but on the fourth day of observation, the tile remained the same consistency as the third
day; which was not enough to be the ideal organic type which we had envisioned as enduring.
20
Table 2: Observation of the odor and durability of the tile from day 1 to 4; for trial 2.
For the first day of observation, the tile had not secreted any evident odor, but did in fact had a
faint stench only distinguishable upon smelling the tile up close, which continued for the following
days to come. In terms of durability, the increase in the added binding agent allowed the mixture to
solidify into a tile. After the second day, the consistency of the tile remained the same, as well as
for the consecutive days that followed. The resulting tile once again, did not meet the expectations
as the tile was of brittle structure although solidified. Compared to the pprevious trials though,
21
4.2 Analysis of Data
The tile seems to be semifluid and can be emulated to the sense of the characteristics of
clay, the approximate size of the tile is 7x7 in. (inches) and weights 1 kg (kilogram), given that it
isn’t as dense as expected; the surface also seems to be rough and rigid. The tile can sustain its
form, to a certain extent that it can be molded like clay, but with a little more labor and force. The
tile seemed to emanate odor after a period of time, and it seems to worsen as days pass by;
22
Chapter V
5.1 Conclusion
After the observation of results, the researchers have picked up certain errors within the
processes attempted. The researchers conclude that one of the causes for the unsuccessful output
of the experimentation conducted is the insufficient amount of binding agent (cornstarch) added in
the mixture for both trials —the component in charge of hardening and binding the tile into a solid
—therefore hindered the hardening of the mixture into a tile. Another factor was the improper
cleansing of the bones, which the researchers’ suspect as the reason for the emanating odor.
After conducting the first trial, wherein the mixture is exposed to direct heat, the
researchers’ have arrived at the realization that it was deemed nonessential for the hardening
process of the mixture. As the mixture had not fully solidify, the resulting output was feeble in
terms of durability. As for the odor, the stench emanated and seemed to reek further as days
passed. The air-drying technique seemed to be the best option, but as stated, the inadequacy of
For the last trial, the resulting tile was brittle in structure, although it did in fact solidify
better than the last trials conducted. The researchers suspect that the reason for the unsuccessful
trial was that the air-drying process did not allot the heat the mixture required for it to be a tile of
durable quality. On the other hand, the researchers have observed that the reason for the
unsuccessful output of the first trial was because the set temperature and amount of time the
mixture had been left in the oven was did not suffice.
23
In conclusion to the hypothesis, the durability and solid structure of the bones allowed it to
be a component in tile making, except the resiliency of the newly created tile had not been tested
due to certain errors that hindered the tile from full solidification. Therefore, a drop test could not
have been made possible because of the resulting tile’s unstable structure which would only
guarantee an unsuccessful result due to the erroneous processes attempted by the researchers.
This is the final output of the experimental process conducted by the researchers:
5.2 Recommendations
The researchers have observed that the main fault in the experiment is the inability to
remove the stench from the bones, eventually becoming unbearable as more time passed. Not
being able to thoroughly clean the bones became a constituent of the problem, the other being the
fact that the researchers weren’t given enough time to actually allow the tile to dry; a time
constraint.
The recommended action on this is by using the method “degreasing” before actually
submerging the bones in a hydrogen peroxide formula. The process includes leaving the bones
inside a basin filled with water and partly dishwashing soap to let the grease leave the bone.
24
Another error is the boiling of the bones; which actually causes fat to get trapped inside the bones,
Another recommended action is to decrease the amount of tap water in the mixture,
along with mixing the hydrogen peroxide with the binding agent beforehand, since its is
The researchers also recommend scheduling the trials and experimentations in an orderly
manner; a clear layout of a schedule for this experimentation surprisingly takes copious amounts of
time, especially the process of cleaning the bones and removing the odour.
The researchers recommend for those who will take interest in this study, to consider the
usage of egg whites as an alternative-binding agent. During the Spanish colonial period, egg whites
were combined with lime, sand, and water to create mortars for the construction of churches.
Mortars are used in masonry construction as a binder (Michelle Eusebio, 2009). But further research
must be done in order to ensure that the egg whites won’t spoil. It is also recommended to attempt
different durability tests, such as the “drop-test” to ensure its resiliency and durability.
25
Bibliography
Awaz, Abrham (2018) “Effects of Varying Dosage Replacement of Cement Content by Animal
http://pubs.sciepub.com/ajcea/6/4/1/index.html
Bommala, Nabinder (2018) “Clandestine operation: Bones from Carcass Cold Meat to Tiles and
operation-bones-from-carcass-cold-meat-for-shine-in-tiles/articleshow/63777195.cms
Chu (2016) “Bones & Seashells Inspire Sustainable Design for Concrete” Retrieved from
https://www.designnews.com/materials-assembly/bones-seashells-inspire-sustainable-design-
concrete/111478494146715
http://www.h2o2.com/products-and-services/us-peroxide
technologies.aspx?pid=112&name=Hydrogen-Peroxide
Cunnings, Herbert (2014) Re: Ask MetaFilter: Querying the Hive Mind, 2014 [Online forum]
Eusebio, Michelle (2009) “Did our Church Builders Use Egg Whites as Mortar Ingredients?: The
Biomolecular Reason Behind Collecting Mortars from Historical Sites” Retrieved from
http://www.academia.edu/3697301/Did_our_Church_Builders_Use_Egg_Whites_as_Mortar_Ingredi
ents_The_Biomolecular_Reason_Behind_Collecting_Mortars_from_Historical_Sites
26
Fernandez, Rudy (2015) “Recycled shells good for tile making — study” Retrieved from
https://www.philstar.com/business/agriculture/2005/03/27/271648/recycled-shells-good-tile-
making-151-study
https://www.thespruce.com/ceramic-flooring-pros-and-cons-1314687
https://www.sciencedirect.com/journal/energy/vol/42/issue/1
https://digitalfire.com/4sight/education/binders_for_ceramic_bodies_345.html
Lewitin (2017) “The Advantages of Using Tile Flooring for Your Home” Retrieved from
https://www.expressflooring.com/blog/the-advantages-of-using-tile-flooring-for-your-home/
Lower, Claire (2017) “How to Clean Animal Bones” Retrieved from https://lifehacker.com/how-
to-clean-animal-bones -1798191351
McClelland, John (2016) “Do Animals Have the same Type of Bones as Humans?, paragraph 6”
https://www.thevictorianemporium.com/publications/history/article/history_of_tiles
Naga, Salma (2014) “Recycling of Fish Bone Ash in the Preparation of Stoneware Tiles” Retrieved
from
https://www.researchgate.net/publication/260124945_Recycling_of_Fish_Bone_Ash_in_the_Prepar
ation_of_Stoneware_Tiles
27
Shrummer, Kenny (2013) “Coal-powered electricity?” Retrieved from
https://www.sciencedirect.com/journal/energy/vol/42/issue/1
https://www.ukessays.com/essays/biology/types-of-modified-starches-biology-essay.php
https://www.slideshare.net/VipinKannan1/biodegradation-of-starch
Watson, James (2016) “Do Animals Have the same Type of Bones as Humans?, paragraph 1”
28