Bioanalyse - Zone Table (Expanded)

Interpretive Standards (mm) Control Zone Diameters (mm)
Antimicrobial Agent Content

Code R I S E. coli S. aureus P. aeruginosa
*Microorganism Disk
(Resistant) (Intermediate) (Susceptible) ATCC 25922 ATCC 25923 ATCC 27853

We Bioanalyse® Limited hereby declare that our AST Discs are to be evaluated according to below given clinical and QC parameters:
Amikacin(a) AK-30 30 µg 19-26 20-26 18-26
*Enterobacteriaceae
*P. aeruginosa
<14 15-16 >17
*Acinetobacter
*Staphylococcus spp.
Amikacin(16) AK-10 10 µg 18-24 --- ---

*Enterobacteriaceae
*P. aeruginosa
<14 15-16 >17
*Acinetobacter
Aminosidin(7) (c) AN 60 µg
*Enterobacteriaceae
*Staphylococcus spp. <15 --- >17
Amoxicillin(7) AX-25 25 µg 22-26,5 --- ---
*Enterobacteriaceae <16 --- >21
*S. pneumoniae
*Anaérobies stricts <16 --- >23
Amoxicillin/
AMC-30 20/10 µg 18-24 28-36 ---
clavulanic acid
*Enterobacteriaceae > 13 14-17 >18
*Haemophilus spp. <19 --- >20
Ampicillin AM-10 10 µg 16-22 27-35 ---
*Enterobacteriaceae
and V. cholerae
<13 14-16 >17
*Staphylococci spp. <28 --- >29
*Enterococcus spp. <16 --- >17
*Haemophilus spp. <18 19-21 >22
*Streptococci
(non-S. pneumonia) <18 19-25 >26
*Streptococci
--- --- >24
(ß-hemolytic only)
Ampicillin/sulbactam SAM-20 10/10 µg 19-24 29-37 ---
*Enterobacteriaceae
*P. aeruginosa
*Acinetobacter <11 12-14 >15
and staphylococci
*Haemophilus spp. <19 --- >20

Apramycin(6) APR-15 15 µg 15-20 17-24 13-18
Azithromycin AZM-15 15 µg --- 21-26 ---
*Staphylococcus spp. <13 14-17 >18
*Haemophilus spp. --- --- >12
*S. pneumoniae and <13 14-17 >18

other streptococci
*Neisseria meningitidis --- --- >20

Azlocillin AZL-75 75 µg --- --- 24-30
*P. aeruginosa <17 --- >18
Aztreonam ATM-30 30 µg 28-36 --- 23-29

*Enterobacteriaceae <17 18-20 >21
*P. aeruginosa
Page 1 / 16
*Microorganism Disk

Bacitracin(5) B-10 10 U --- 12-22 ---

*E. coli
*P. aeruginosa <8 9-12 >13
*S. aureus
Carbenicillin PY-100 100 µg 23-29 --- 18-24
*Enterobacteriaceae
<19 20-22 >23
and Acinetobacter
*P. aeruginosa <13 14-16 >17

Cefaclor CEC-30 30 µg 23-27 27-31 ---
*Enterobacteriaceae
and Staphylococcus <14 15-17 >18
spp.
Cefadroxil(7) CFR-30 30 µg --- --- ---

Cefadroxil(18) CFR-30 30 µg 14-20 --- ---

Cefamandole MA-30 30 µg 26-32 26-34 ---
*Enterobacteriaceae
and Staphylococcus <14 15-17 >18
spp.
Cefazolin CZ-30 30 µg 21-27 29-35 ---
*Enterobacteriaceae
<19 20-22 >23
Cefdinir CD-5 5 µg 24-28 25-32 ---


Cefepime FEP-30 30 µg 31-37 23-29 24-30
*Enterobacteriaceae,
*P. aeruginosa,
<14 15-17 >18
*Acinetobacter
and staphylococci,

*N. gonorrhoeae. --- --- > 31
*Viridans streptococci
<21 22-23 >24
(non-S. pneumoniae)
*Streptococci
(non-S. pneumoniae, --- --- <24
ß Hemolytic only
Cefixime CFM-5 5 µg 23-27 --- ---

Cefodizime(14) CDZ-30 30 µg --- --- ---

*N. gonorrhoeae --- --- >31
Cefonicid CID-30 30 µg 25-29 22-28 ---

Page 2 / 16
*Microorganism Disk

Cefoperazone CEP-75 75 µg 28-34 24-33 23-29
*Enterobacteriaceae
*P. aeruginosa
and Staphylococcus
spp.
Cefoperazone/ (13) CES-105 75/30 µg --- --- ---

sulbactam
*Enterobacteriaceae
<14 15-19 >20
*Acinetobacter
Cefotaxime(18) CTX-5 5 µg 25-31 --- ---
Cefotaxime CTX-30 30 µg 29-35 25-31 18-22

*P. aeruginosa
*Acinetobacter and <14 15-22 >23
Staphylococcus spp.

*N. meningitidis --- --- >34

*Streptococci (non-S.
pneumoniae, --- --- >24
ß-Hemolytic only)
*Viridans Streptococci
(non-S. pneumoniae) <25 26-27 >28
Cefotaxime/ (e)
CTC-40 30/10 µg --- --- ---
clavulanic acid
Cefotetan CTT-30 30 µg 28-34 17-23 ---
*Enterobacteriaceae,
<12 13-15 >16
*N. gonorrhoeae <19 20-25 >26
Cefoxitin FOX-30 30 µg 23-29 23-29 ---

*Staphylococcus
<21 --- >22
aureus
Cefpirome(7) CPO-30 30 µg --- --- ---

P. aeruginosa <19 --- >19
*Acinetobacter <19 --- >21
Cefpodoxime CPD-10 10 µg 23-28 19-25 ---
*Enterobacteriaceae
<17 18-20 >21
Cefprozil CPR-30 30 µg 21-27 27-33 ---
and Staphylococcus
Page 3 / 16
*Microorganism Disk

Cefquinome CEQ-30 30 µg 28-36 25-33 ---
Cefsulodin(17) CFS-30 30 µg --- --- ---

(18) CAZ-10 10 µg 23-29 --- ---
Ceftazidime
Ceftazidime CAZ-30 30 µg 25-32 16-20 22-29

*P. aeruginosa
*Staphylococcus spp. < 14 15-17 > 18
and Acinetobacter
*Enterobacteriaceae < 17 18-20 >21
*B. cepacia < 17 18-20 >21
Ceftazidime/(e) CZC-40 30/10 --- --- ---
clavulanic acid µg
Ceftibuten CBT-30 30 µg 27-35 --- ---
(3) FUR-30 30 µg 26-31 27-31 14-18
Ceftiofur
*E. coli
*S. aureus <17 18-20 >21
*Streptococcus spp.
Ceftizoxime ZOX-30 30 µg 30-36 27-35 12-17
Ceftriaxone CRO-30 30 µg 29-35 22-28 17-23
*P. aeruginosa
<13 14-20 >21
*Acinetobacter and
Staphylococci
*N. gonorrhoeae -- --- >35
*Viridans Streptococci < 24 25-26 >27
(non-S. pneumoniae)

*Streptococci
(non-S. pneumoniae, --- --- >24
β-Hemolytic only)
Cefuroxime (sodium) CXM-30 30 µg 20-26 27-35 ---
*Enterobacteriaceae
and staphylococci <14 15-17 >18
(parenteral)

*N. gonorrhoeae <25 26-30 > 31
Cefuroxime (axetil) CXA-30 30 µg 20-26 27-35 ---
*Enterobacteriaceae
<14 15-22 >23
and staphylococci (oral)
Page 4 / 16
*Microorganism Disk

Cephalexin(10) CL-30 30 µg --- --- ---

Cephalexin(18) CL-30 30 µg 15-21
Cephalothin KF-30 30 µg 15-21 29-37 ---

*Enterobacteriaceae
and staphylococcus <14 15-17 >18
spp.
Cephradine(17) CE-30 30 µg --- --- ---
*Streptococcus spp. <12 --- >18
Chloramphenicol
C-30 30 µg 21-27 19-26 ---
(According to CLSI)
*Enterobacteriaceae
* P. aeruginosa
*Acinetobacter
*Staphylococci
<12 13-17 >18
* V. cholerae
*Enterococci
*Haemophilus spp. < 25 26-28 >29
*N. meningitidis <19 20-25 >26
*S. pneumonia <20 --- >21
*Streptococcus spp.
(non-S. pneumoniae)
<17 18-20 >21
Chloramphenicol (7)
C-30 30 µg --- --- ---
(According to SFM)
*Enterobacteriaceae
*P. aeruginosa
*Acinetobacter
<19 --- >23
Cinoxacin CIN-100 100 µg 26-32 --- ---
Ciprofloxacin CIP-5 5 µg 30-40 22-30 25-33
*Enterobacteriaceae
(except for Salmonella
spp.)
*P. aeruginosa
<15 16-20 >21
*Acinetobacter
*Enterococcus spp.
*Enterobacteriaceae
(with Salmonella spp.)
< 20 21-30 >31
*N. meningitidis < 32 33-34 >35
*N. gonorrhoeae < 27 28-40 >41
Clarithromycin CLR-15 15 µg --- 26-32 ---
*S. pneumoniae and

other streptococci
<16 17-20 > 21
Clindamycin DA-2 2 µg --- 24-30 ---
*S. pneumoniae and
other streptococci
<15 16-18 >19
Cloxacillin(10) CX-5 5 µg --- 26-32 ---
*S. aureus
<10 11-12 >13
Page 5 / 16
*Microorganism Disk

Colistin CT-10 10 µg 11-17 --- 11-17
*P. aeruginosa < 10 --- >11
Colistin(7) CT-50 50 µg --- --- 17-22

*Enterobacteriaceae
*Acinetobacter
Danofloxacin DAN-5 5 µg 29-36 24-31 18-25
*Pasteurella multocida
--- --- >22
*M. haemolytica
Dirithromycin DTM-15 15 µg --- 18-26 ---
*S. pneumonia
*Streptococcus spp. <13 14-17 > 18
(β-hemolytic group)
Dibekacin(17) DBK-10 10 µg --- --- ---
*Enterococcus spp.
*Staphylococcus spp. ---
(S. pneumoniae is
<14 >16
excepted)
Doripenem DOR-10 10 µg 27-35 33-42 28-35

*P. aeruginosa <15 16-18 >19
Doxycycline DO-30 30 µg 18-24 23-29 ---
*P. aeruginosa
<12 13-15 >16
and Enterococci
<24 25-27 >28

*S. pneumonia
Enoxacin EN-10 10 µg 28-36 22-28 22-28
*Enterobacteriaceae
and Staphylococcus
<14 15-17 >18
Enrofloxacin(2) ENR-5 5 µg 32-40 27-31 15-19
*Mannheimia
haemolytica
*Pasteurella multocida <16 17-19 >20
*Histophilus somni
Ertapenem ETP-10 10 µg 29-36 24-31 13-21

Erythromycin E-15 15 µg --- 22-30 ---
and enterococci
<13 14-22 >23
*S. pneumoniae and
other Streptococcus <15 16-20 >21
spp.
Flucloxacillin(f) FL-5 5 µg --- --- ---

Flumequine(7) FLM-30 30 µg --- --- ---
*Enterobacteriaceae < 21 --- > 25
Fosfomycin FF-200 200 µg 22-30 25-33 ---
*E. coli and E. faecalis <12 13-15 >16
Page 6 / 16
*Microorganism Disk

Framycetin(7)(c) FM-100 100 µg --- --- ---

*Enterobacteriaceae
Staphylococcus spp.
<15 --- <17
Furazolidone(10) FX-50 50 µg --- --- ---
<14 15-18 >17
Fusidic Acid(10) FA-10 10 µg

Fusidic Acid
FA-10 10 µg --- 24-32 ---
(According to CLSI)
Florfenicol (3) FFC-30 30 µg 22-28 22-29 ---

*Mannheimia
haemolytica
*Pasteurella multocida <14 15-18 >19
*Histophilus somni
*B. bronchiseptica
*S. suis
*Salmonella
<18 19-21 >22
choleraesuis
Garenoxacin GAN-5 5 µg 28-35 30-36 19-25
Gatifloxacin GAT-5 5 µg 30-37 27-33 20-28

*Streptococcus spp. <19 20-22 >23
*P. aeruginosa
<14 15-17 >18
*Acinetobacter
*Enterococcus spp.
<14 15-17 >18
*H. influenzae and H.

--- --- >18
parainfluenzae
*N. gonorrhoeae <33 34-37 > 38
*S. pneumoniae and
<17 18-20 >21
other streptococci
Gentamicin(g) CN-120 120 µg --- --- ---
*Testing enterococci
(for high level <6 7-9 >10
resistance)
Gentamicin CN-10 10 µg 19-26 19-27 17-23
*Enterobacteriaceae
*P. aeruginosa
*Staphylococci
<12 13-14 >15
*Acinetobacter
Imipenem IPM-10 10 µg 26-32 --- 20-28
*Acinetobacter
<13 14-15 >16
*P. aeruginosa <15 16-18 >19
Isepamicin(17) ISP-30 30 µg --- --- ---
*Enterobacteriaceae
*Acinetobacter spp. <15 --- >17
*P. aeruginosa
Kanamycin (a) K-30 30 µg 17-25 19-26 ---
*Enterobacteriaceae
<13 14-17 >18
Kanamycin(7) K-30 10 IU --- --- ---
*Enterobacteriaceae
<15 --- >17
Page 7 / 16
*Microorganism Disk

Levofloxacin LEV-5 5 µg 29-37 25-30 19-26

*Enterobacteriaceae
*P. aeruginosa <13 14-16 >17
*Acinetobacter
*Enterococci
*Staphylococci <15 16-18 >19
*S. pneumoniae and
other streptococci
(non-S. pneumoniae, <13 14-16 >17
ß-Hemolytic only)
Lomefloxacin LOM-10 10 µg 27-33 23-29 22-28
*Enterobacteriaceae
*P. aeruginosa
<18 19-21 >22
*Acinetobacter
Linezolid LNZ-30 30 µg --- 25-32 ---
---
*Staphylococcus spp. < 20 >21
*S. pneumoniae
and other streptococci
--- --- >21
*Enterococcus spp. < 20 21-22 >23
Loracarbef LOR-30 30 µg 23-29 23-31 ---
*Enterobacteriaceae
<14 15-17 >18
Marbofloxacin MAR-5 5 µg 29-37 24-30 20-25
*Enterobacteriaceae
<14 15-19 >20
Mecillinam(7) MEC-10 10 µg --- --- ---
Mecillinam(18) MEC-10 10 µg 24-30 --- ---
Meropenem MEM-10 10 µg 28-34 29-37 27-33

*P. aeruginosa <15 16-18 >19
Methicillin ME-5 5 µg --- 17-22 ---
*Staphylococcus
< 10 11-12 >13
aureus
Mezlocillin MEZ-75 75 µg 23-29 --- 19-25
* Acinetobacter <17 18-20 >21
Minocycline MI-30 30 µg 19-25 25-30 ---

*P. aeruginosa
*Enterococci
*Acinetobacter
<12 13-15 >16
*Enterobacteriaceae
Moxalactam MOX-30 30 µg 28-35 18-24 17-25
<14 15-22 >23
Page 8 / 16
*Microorganism Disk

Moxifloxacin MXF-5 5 µg 28-35 28-35 17-25

*H. influenzae
--- --- >18
*H. parainfluenzae
*S. pneumoniae <14 15-17 >18
Mupirocin(7) MUP-5 5 µg --- --- ---
* Staphylococcus spp.
*S. saprophyticus
*S. xylosus --- --- >19
*S. cohnii
(intrinsic resistance)
Nafcillin NAF-1 1 µg --- 16-22 ---
*Staphylococcus
<10 11-12 >13
aureus
Nalidixic Acid NA-30 30 µg 22-28 --- ---
*Enterobacteriaceae < 13 14-18 >19

Netilmicin(18) NET-10 10 µg 18-24 --- 15-21
Netilmicin NET-30 30 µg 22-30 22-31 17-23

*Enterobacteriaceae
*P. aeruginosa <12 13-14 >15
Neomycin(3) (a) N-30 30 µg 17-23 18-26 ---
*Enterobacteriaceae
<12 13-16 >17
(5) NV-30 30 µg --- 22-31 ---
Novobiocin
(Muller Hinton agar with
sheep blood for <14 15-16 >17
veterinary) in use
Nitrofurantoin(18) F-100 100 µg 17-23 --- ---
Nitrofurantoin F-300 300 µg 20-25 18-22 ---

*Enterobacteriaceae
*Enterococcus spp.
Norfloxacin NOR-10 10 µg 28-35 17-28 22-29
*Enterobacteriaceae
*P. aeruginosa
*Enterococcus spp.
Ofloxacin OFX-5 5 µg 29-33 24-28 17-21
*Enterobacteriaceae
*P. aeruginosa <12 13-15 >16
*Acinetobacter and
*S. pneumoniae and

other streptococci
<12 13-15 >16
(non-S. pneumoniae,
ß-Hemolytic)
Page 9 / 16
*Microorganism Disk

Oleandomycin(11) OL-15 15 µg --- --- ---
<11 12-16 >17
Oxacillin OX-1 1 µg --- 18-24 ---

*S. aureus <10 11-12 >13
<17 --- >18
(coagulase-negative)
*S. pneumonia
--- --- >20
(for penicillin G
susceptibility)
Oxolinic acid (5) OA-2 2 µg 20-24 10-13 ---
Oxolinic acid (a) OA-2 2 µg ---
*Aeromonas
<24 25-29 >30
salmonicida
Oxytetracycline T-30 30 µg --- --- ---
*Streptococcus spp.
<14 15-18 >19
*Enterobacteriaceae
*Streptococcus
pneumonia <18 19-22 >23
*Streptococcus spp.
*Aeromonas
< 21 22-27 >28
salmonicida
Penicillin P-10 10 --- 26-37 ---

*Staphylococcus spp. < 28 --- >29
*Enterococcus spp. <14 --- >15
*Streptococci
<19 20-27 >28
(non-S. pneumoniae)
*Streptococcus spp. --- --- >24
*S. pneumoniae --- --- >20
Piperacillin/
TPZ-36 36 µg 21-27 --- 23-29
tazobactam (18)
Piperacillin/ 100
TPZ-100/10 24-30 27-36 25-33
tazobactam /10
*Enterobacteriaceae
<17 18-20 >21
*Acinetobacter spp.
>21
*P. aeruginosa <14 15-20
*H. influenza
--- --- >21
*H. parainfluenzae
Piperacillin PRL-30 30 µg 21-27 --- ---
Piperacillin PRL-100 100 µg 24-30 --- 25-33

*Enterobacteriaceae
<17 18-20 >21
*Acinetobacter spp.
*P. aeruginosa <14 15-20 >21
Pefloxacin(7) PEF-5 5 µg 29-35.5 25.5-29.5 ---
*P. aeruginosa
Pipemidic acid (2) PI-20 20 µg --- --- ---
*Enterobacteriaceae <10 11-13 > 14
Pirlimycin(1) (3) PIR-2 2 µg --- 15-19 ---
*S. aureus
≤ 12 --- ≥ 13
*Streptococcus spp.
Polymixin B (5) PB-300 300 U 12-16 --- ---
*P. aeruginosa
<8 9-11 ≥12
*Acinetobacter
Page 10 / 16
*Microorganism Disk

CLSI 13-19 14-18

*P. aeruginosa
<11 ≥12
Pristinamycin(7) PT-15 15 µg --- 26.5-32 ---

*Enterococcus spp.
<19 --- ≥ 22
*Streptococcus
pneumoniae
Quinupristin/ 4.5/10.
SYN-15 --- 21-28 ---
dalfopristin 5 µg
*Enterococcus spp.
*Streptococcus spp. <15 16-18 >19
*S. pneumoniae
Rifampin RA-5 5 µg 8-10 26-34 ---

<16 17-19 >20
*Enterococcus spp.
*S. pneumonia <16 17-18 >19
Roxithromycin(15) ROX-15 15 µg --- --- ---
*Enterococci <9 10-20 >21
Sparfloxacin SPX-5 5 µg 30-38 27-33 21-29
*S. pneumoniae <15 16-18 >19
Spectinomycin SPT-100 100 µg --- --- ---
Streptomycin S-10 10 µg 12-20 14-22 ---
Streptomycin (g) S-300 300 µg --- --- ---
Testing enterococci
<6 7-9 >10
for high level resistance
Spiramycin(7) SP-100 100 --- --- ---
µg
* S. pneumonia <19 --- >24
*Streptococcus spp.
and anaerobes
Sisomycin(7) SIS-10 10 µg --- --- ---
*Enterococcus spp.
<14 --- >6
*Streptococcus spp.
Sulfisoxazole ST-300 300 µg 15-23 24-34 ---
*Enterobacteriaceae
*P. aeruginosa
*Staphylococci
*V. cholerae
Sulfamethoxazole(10) SMZ-50 50 µg --- --- ---
<11 12-25 >16
Teicoplanin TEC-30 30 µg --- 15-21 ---
<10 11-13 >14
*Enterococcus spp.
Telithromycin TEL-15 15 µg --- 24-30 ---
*S. pneumoniae <15 16-18 >19
Page 11 / 16
*Microorganism Disk

Tetracycline TE-30 30 µg 18-25 24-30 ---

*P. aeruginosa,
*Enterococci and V. <14 15-18 >19
Cholerae
*Acinetobacter
<11 12-14 >15
*Enterobacteriaceae
*S. pneumoniae and
<18 19-22 >23
Streptococcus spp.
Ticarcillin TIC-75 75 µg 24-30 --- 21-27
*Enterobacteriaceae
<14 15-19 >20
*Acinetobacter
*P. aeruginosa <15 16-23 >24
Tigecycline(9) TGC-15 15 µg 20-27 20-25 9-13
*S. aureus --- --- >19
*Streptococcus spp.
--- --- >19
* S. pneumoniae
* Enterococcus faecalis
(Vancomycin- --- --- >19
susceptible)
Thiamphenicol(7)(b) TP-30 30 µg
(According to SFM)
*Enterobacteriaceae
*P. aeruginosa
*Streptococcus spp.
Tilmicosin(3) TIL-15 15 µg --- 17-21 ---

*Actinobacillus
pleuropneumoniae <10 --- >11
*Pasteurella multocida
*M. haemolytica <10 11-13 >14

Ticarcillin/ 75/10
TIM-85 24-30 29-37 20-28
clavulanic acid µg
*Enterobacteriaceae
<14 15-19 >20
and Acinetobacter
*P. aeruginosa <15 16-23 >24
Tobramycin TOB-10 10 µg 18-26 19-29 20-26
*Enterobacteriaceae
*P. aeruginosa
<12 13-14 >15
*Acinetobacter and
Staphylococcus spp.
Trimethoprim/ 1.25 /
SXT-25 23-29 24-32 ---
sulfamethoxazole 23.75
*Enterobacteriaceae
*P. aeruginosa
*V. cholerae
*S. pneumoniae <15 16-18 >19
Trimethoprim TMP-5 5 µg 21-28 19-26 ---
*Enterobacteriaceae
<10 11-15 >16
Page 12 / 16
*Microorganism Disk

Triple Sulphonamides S3-300 300 µg --- --- ---
*Enterobacteriaceae
<12 13-16 >17
Tulathromycin TUL-30 30 µg --- 18-24 ---
*M. haemolytica
*P. multocida <14 15-17 >18
*Histophilus somni
Tylosin(10)(6) TY-30 30 µg --- 18-24 ---
<16 --- >20
Vancomycin VA-30 30 µg --- 17-21 ---
*Staphylococcus spp. --- --- > 15
*Enterococcus spp. <14 15-16 >17
*S. pneumoniae and
other Streptococcus --- --- >17
spp.
Metronidazole(8) MET-5 5 µg
*for anaerobic (H. pylori) ---
< 16 >21
organisms
Page 13 / 16

Interpretive Standards (mm) Control Zone Diameter Limits (mm)

Content
Antifungal Agent Code
Disk C. albicans C. parapsilosis C. tropicalis
R I S
ATCC 90028 ATCC 22019 ATCC 750
Clotrimazole (10) CLT-50 50 µg 20-31 26-35 30-39

Candida spp. <10 11-19 >20
Econazole(10) ECO-10 10 µg 22-28
Candida spp. <19 --- >20
Fluconazole
FLU-25 25 µg 28-39 22-33 26-37
(According to CLSI)
Candida spp. <14 15-18 >19
Miconazole(10) MCZ-10 10 µg 21-29
Candida spp. <16 --- ---
Voriconazole
VOR-1 1 µg 31-42 28-37 ---
(According to CLSI)
Candida spp. <13 14-16 >17
Amphotericin B AMB-20 20 µg 19-24 21-27
(According to CLSI)
Candida spp.
Amphotericin B(10) AMB-20 20 µg
Candida spp. < 14 --- > 15
Caspofungin
CAS-5 5 µg 18-27 14-23 20-27
(According to CLSI)
Interpretive Standards (mm) Control Zone Diameter Limits (mm)

Content
Antifungal Agent Code
Disk C. albicans C. parapsilosis C. krusei
R I S
ATCC 90028 ATCC 22019 ATCC 6258
Clotrimazole (10) CLT-50 50 µg 20-31 26-35 28-36

Candida spp. <10 11-19 >20
Ketoconazole(10) KTC-10 10 µg 29-39 41-49 20-25
Candida spp. <19 --- >20
Nystatine(10) NY-100 100 U 18-26 19-26 14-21
Candida spp. < 17 --- >18
Itraconazole(10) ITR-50 50µg 11-22 8-15 8-15
Candida spp. < 10 11-19 > 20
Page 14 / 16

Hints :
(a) FDA- approved zone size recommendations from drug manufacturers not included in NCCLS M100-S13(M2-A8)
(b) According to COMITE DE L’ANTIBIOGRAMME DE LA SOCIETE FRANCAISE DE MICROBIOLOGIE (SFM) REPORT 2003 the interpretive
range for Chloramphenicol is also valid for Thiamphenicol.

(c) It is given by SFM that the interpretation for Kanamycin is valid for Neomycin, Framycetin, Aminosidin (Paromomycin)
(d) According to SFM the interpretation for Colistin is valid for Polymixin B.
(e) Find in reference 13. Screening breakpoints are: Ceftazidime (<22 mm), Cefotaxime (<27 mm). Quality Control recommendations are:
E. coli ATCC 25922 (as given at the table), K. pneumoniae ATCC 700603 Ceftazidime (10-18 mm), Cefotaxime (17-25 mm).The use of more
than one antimicrobial agent for screening improves the sensitivity of detection. Phenotypic conformity testing requires the use of both
Ceftazidime and Cefotaxime alone and in combination with Clavulanic acid. Quality control recommendations are: negative strain E. coli ATCC
25922 which produces a “<2 mm” increase in zone diameter for antimicrobial agent tested alone versus its zone diameter when tested in
combination clavulanic acid; positive strain for K. pneumoniae ATCC 700603, which produces a “>3 mm” increase in Cefotaxime zone diameter
and “>5 mm” in Ceftazidime zone diameter.
(See ‘Limitations of the procedure’ for details of procedure.)
(f) According to CLSI, Oxacillin is the preferred agent when a Penicillinase-stable Penicillin is tested and results can be applied to the other
Penicillin-stable Penicillins, Cloxacillin, Dicloxacillin, Flucloxacillin.

(g) According to CLSI, for control limits of gentamicin 120 mcg and streptomycin 300 mcg disks, use E.faecalis ATCC 29212 (gentamicin ; 16-23
mm; streptomycin: 14-20 mm).

References
(1) NADA # 141-036, Approved by FDA
Pharmacia & Upjohn Company
Kalamazoo, Michigan 49001, USA
Revised January 2003
(2) Department of Microbiology and Infectious Diseases, Veterinary Faculty University of Zagreb, Zagreb, Croatia
In Vitro Antimicrobial Susceptibility of 183 Pseudomonas aeruginosa Strains Isolated from Dogs to Selected Antipseudomonal
Agents
B. S EOL, T. NAGLIC , J. MADIC and M. BEDEKOVIC
Pg: 2/5
(3) The Veterinary Antimicrobial Decision Support System
Mike Apley, DVM, PhD Department of Clinical Sciences

Kansas State University
Pgs: 11-12-13-14/22
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(M100-S23 Vol. 33 No. 1)

(5) BD BBLTM Sensi-DiskTM Antimicrobial Susceptibility Test Discs
(6) Performance Standards for Antimicrobial Susceptibility Testing from Bacteria Isolated from Animals; Approved Standard
Third Edition
(M31-A13 Volume.28 No.8 Replaces M31-A2 Volume.22 No.6)
(7) COMITE DE L’ANTIBIOGRAMME DE LA SOCIETE FRANCAISE DE MICROBIOLOGIE RECOMMANDATIONS 2013 (SFM)
(Chairholder: Pr C.J. SOUSSY Centre Hospitalier Universitaire Henri Mondor 94010 Créteil Cedex)
(8) Assessment of Metronidazole Susceptibility in Helicobacter Pylori: Statistical Validation and Error Rate Analysis of Breakpoints
Determined by the Disk Diffusion Test (Journal of Clinical Microbiology)

(January, 1999)
(9) PRODUCT MONOGRAPH
‘TYGACIL’ Tigecycline for Injection Sterile, lyophilized powder for Intravenous use 50 mg/ Vial Tetracycline Antibiotic
(glycylcycline derivative)
Montreal, Canada
Date of Revision:
August 15, 2007
Submission Control Number: 114369
Pgs: 33-34/63
(10) ORGENICS
ANTIBIOTIC DISC
Disc of Absorbent paper impregnated with Antibiotic and utilized for susceptibility testing
(Kirby Bauer Method).
Bauer, A.W., Kirby W.M.M., Sherris, K.R. and Truck, M.(1996) Amor, J.Clin.Path.45,
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NCCLS “Performance Standards for Antimicrobial Disk Susceptibility Tests;
Approved Standard – Seventh Edition, January 2000
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Eleventh Informational Supplement, January 2001.
WHO (1977) Tech.Rep.Ser.,No.810
FDA (1978) Codes of Fed.Rebs.21 Pag.460
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A CENTENARY PERSPECTIVE
Edited by: Wolfgang K. Joklik, Lars G. Ljunkgdahl, Alison D. O’Brian, Alexander von Graevenitz, Charles Yanofsky
With a Foreword by Joshua Lederberg
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(13) Comparison of Disk Susceptibility Test of Gram-Negative Rods with MIC for Cefoperazone-Sulbactam Combination.
Fatima Noman, Uzma Usman, Altaf Ahmed. Department of Microbiology, Liaquat National Hospital, Karachi.
Jan - Mar 2007.5
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Mary S. Barrett, Ronald N. Jones and Meridith E. Erwin

Department of Pathology, Anti-Infectives Research Center, University of Iowa Collage of Medicine, Iowa City, Iowa USA
Received 13 April 1993; accepted 8 June 1993. Available online 14 November 2002.
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of Pathogenic E. coli Isolated from Children Age Range 1-11 in Metro Manila. Phil J. Microbiol Infect Dis 1984; 13(2):74-87.
(17) COMITE DE L’ANTIBIOGRAMME DE LA SOCIETE FRANCAISE DE MICROBIOLOGIE RECOMMANDATIONS 2003 (SFM)
(Chairholder: Pr C.J. SOUSSY Centre Hospitalier Universitaire Henri Mondor 94010 Créteil Cedex)
(18) European Society of Clinical Microbiology and Infectious Diseases Version 3.1, valid form 2013-02-11 (EUCAST)
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Bioanalyse - Zone Table (Expanded)

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Interpretive Standards (mm) Control Zone Diameters (mm)

Antimicrobial Agent Content

Amikacin(a) AK-30 30 µg 19-26 20-26 18-26

Amikacin(16) AK-10 10 µg 18-24 --- ---

*Haemophilus spp. <19 --- >20

*S. pneumoniae and <13 14-17 >18

*Neisseria meningitidis --- --- >20

*P. aeruginosa <17 --- >18

Aztreonam ATM-30 30 µg 28-36 --- 23-29

Bacitracin(5) B-10 10 U --- 12-22 ---

*P. aeruginosa <13 14-16 >17

*Haemophilus spp. <16 17-19 >20

Cefadroxil(7) CFR-30 30 µg --- --- ---

*Staphylococcus spp. <14 15-17 >18

Cefdinir CD-5 5 µg 24-28 25-32 ---

*Haemophilus spp. --- --- >20

*Haemophilus spp. --- --- >26

*Enterobacteriaceae <15 16-18 >19

*Haemophilus spp. <16 17-19 >20

*Staphylococcus spp. <14 15-17 >18

Cefoperazone CEP-75 75 µg 28-34 24-33 23-29

Cefoperazone/ (13) CES-105 75/30 µg --- --- ---

Cefotaxime(18) CTX-5 5 µg 25-31 --- ---

Cefotaxime CTX-30 30 µg 29-35 25-31 18-22

*Enterobacteriaceae <22 23-25 >26

*N. meningitidis --- --- >34

*N. gonorrhoeae --- --- >31

Cefoxitin FOX-30 30 µg 23-29 23-29 ---

*Enterobacteriaceae <17 --- >24

Cefquinome CEQ-30 30 µg 28-36 25-33 ---

Cefsulodin(17) CFS-30 30 µg --- --- ---

Ceftazidime CAZ-30 30 µg 25-32 16-20 22-29

*Haemophilus spp. <16 17-19 >20

Cephalexin(10) CL-30 30 µg --- --- ---

Cephalexin(18) CL-30 30 µg 15-21

Cephalothin KF-30 30 µg 15-21 29-37 ---

*S. pneumoniae and

Colistin CT-10 10 µg 11-17 --- 11-17

*P. aeruginosa < 10 --- >11

Colistin(7) CT-50 50 µg --- --- 17-22

*Enterobacteriaceae <19 20-22 >23

*Acinetobacter <9 10-12 >13

<24 25-27 >28

Ertapenem ETP-10 10 µg 29-36 24-31 13-21

Flucloxacillin(f) FL-5 5 µg --- --- ---

Framycetin(7)(c) FM-100 100 µg --- --- ---

<14 15-18 >17

Fusidic Acid(10) FA-10 10 µg

Florfenicol (3) FFC-30 30 µg 22-28 22-29 ---

Gatifloxacin GAT-5 5 µg 30-37 27-33 20-28

*H. influenzae and H.

Levofloxacin LEV-5 5 µg 29-37 25-30 19-26

Meropenem MEM-10 10 µg 28-34 29-37 27-33

* Acinetobacter <17 18-20 >21

Minocycline MI-30 30 µg 19-25 25-30 ---

Moxifloxacin MXF-5 5 µg 28-35 28-35 17-25

*S. pneumoniae <14 15-17 >18

Mupirocin(7) MUP-5 5 µg --- --- ---

Nafcillin NAF-1 1 µg --- 16-22 ---