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Assignment

Submitted To:

Dr Ishaq Shb

Submitted By:

M Atta Ur Rahim

Reg. No:

2018-UAM-835

Course Code:

Micro(301)

Topic:

Phylum of Verrucomicrobia & Fusobacteria

Muhammad Nawaz Sharif University of Agriculture, Multan.


Phylum of Verrucomicrobia
Scientific Classification:

Domain: Bacteria

Superphylum: PVC group

Phylum: Verrucomicrobia

 Verrucomicrobia is a phylum of bacteria.This phylum contains only a few described species


Verrucomicrobium spinosum
 The species identified have been isolated from fresh water, marine and soil environments
and human faeces.
 A number of as-yet uncultivated species have been identified in association with
eukaryotic hosts including extrusive explosive ectosymbionts of protists and
endosymbionts of nematodes residing in their gametes.
 While verrucae is another name for the warts often found on hands and feet, this phylum
is so called not because it is a causative agent thereof, but because of its wart-like
morphology.
 Evidence suggests that verrucomicrobia are abundant within the environment, and
important (especially to soil cultures). This phylum is considered to have two sister
phyla: Chlamydiae and Lentisphaerae within the PVC group.
 The Verrucomicrobia phylum can be distinguished from neighbouring phyla within
the PVC group by the presence of several conserved signature indels (CSIs).
 These CSIs represent unique, synapomorphic characteristics that suggest common
ancestry within Verrucomicrobia and an independent lineage amidst other bacteria .
 CSIs have also been found that are shared by Verrucomicrobia and Chlamydia e
exclusively of all other bacteria.
 These CSIs provide evidence that Chlamydiae is the closest relative to Verrucomicrobia ,
and that they are more closely related to one another than to the Planctomycetales.
 Cavalier-Smith has postulated that the Verrucomicrobia belong in
clade Planctobacteria in the larger clade Gracilicutes( controversial taxon in bacterial
taxonomy).
Phylum Fusobacteria:
 The phylum Fusobacteria is described in part on the basis of phylogenetic analyses of
the 16S rRNA gene sequences of its members.
 The phylum contains rod-shaped bacteria that stain Gram-negative.
 Described species are fermentative and produce a variety of organic acids when grown
on carbohydrates, amino acids or peptides.
 Some species are pathogenic to humans.
 After 48 hours’ incubation at 37°C colonies are raised, irregular and have a crenated
edge.
 Microscopic examination of Gram - stained organisms show very long slender rods,
wider at the centre and tapering towards the end.
 Pleomorphism is the rule with coccobacillary and long, straight, slender rods being
found.
 The fusobacteria are strict anaerobes sensitive to metronidazole and tolerant of bile and
gentian violet.
 They are non-spore-forming and are usually non-motile. Fusobacteria are sensitive to
vancomycin , kanamycin and penicillin.
 Leptotrichia buccalis may be isolated from human infection although its role is
controversial. It is a commensal organism found in the mouth.
 The Gram morphology is characteristic with Gram-negative rods up to 15 μm long. The
organisms are metronidazole sensitive and bile tolerant.
 Fusobacteria are found in mixed infections along with spirochaetes in the destructive
facial condition cancrum oris or Ludwig's angina.
 They also participate in mixed infection with Borellia vincentii to give severe mouth
ulceration known as Vincent's angina.
 They are also found in dental and periodontal abscesses, gingivitis, liver abscesses,
cerebral abscess, in anaerobic cellulitis, and necrotizing fasciitis.

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