pBR322

© 2013 Elsevier Inc. All rights reserved.

This article is reproduced from the previous edition, volume 3, p 1428, © 2001, Elsevier Inc., with revisions made by the Editor.

pBR322 is a double-stranded, circular, 4361 base pair plasmid See also: Compatibility Group; Plasmids.
cloning vector (Figure 1). pBR322 was the first artificial plasmid,
containing the ampR gene (encoding ampicillin resistance)
derived from plasmid RSF2124, the tetR gene (encoding tetracy­ Further Reading
cline resistance) derived from plasmid pSC101, and the origin of
replication from pMB1 (a plasmid in the ColE1 compatibility Balbás P and Bolívar F (2004) Back to basics: pBR322 and protein expression systems in
group). pBR322 also encodes the rop gene product, which reg­ E. coli. Methods in Molecular Biology 267: 77–90.
ulates plasmid replication by stabilizing the interaction between Balbas P, Soberon X, Bolivar F, and Rodriguez RL (1988) The plasmid, pBR322.
Biotechnology 10: 5–41.
RNAI and RNAII transcripts, and thereby maintains the plasmid
at ∼20 copies per bacterium. To facilitate its use as a cloning
vector, pBR322 was constructed to have unique sites for a variety
of useful restriction endonucleases. pBR322 was created by Relevant Websites
Bolivar and Rodriguez in 1977, where p stands for plasmid, B
for Bolivar, and R for Rodriguez. pBR322 was one of the most http://ecoliwiki.net – EcoliWiki; pBR322.

widely used plasmid cloning vectors. The complete DNA http://www.neb.com – New England BioLabs Inc.; pBR322 Vector.

sequence is available via GenBank (Accession number: J01749). http://genome-www.stanford.edu/vectordb/vector_descrip/PBR322.html – pBR322.

Cla I - BspD I 23
EcoR I - Apo I 4359 Hind III 29
Aat II 4284 EcoR V 185
BclV I 4209
BsrB I 4205 Nhe I 229
Ssp I 4168 BamH I 375
Ear I 4155 SgrA I 409
Eco57 I 4048 Ban II 471
Ban II 485
Hine II 3905 Xmn I 3961 Btg I 528
Sca I 3844 Sph I 562
Bts I 3787 EcoN I 622
Bts I 3759 Sal I 651
pυu I 3733 Hine II 651
pst I 3607 Acc I 651
BsrD I 3602
Tc
Ap

PshA I 712
Ase I 3537

Bsa I 3433 Eag I 939

BsrD I 3420 Nru I 972
Ahd I 3361 BstAP I 1045
BspM I 1063

PfIM I 1315
Eco57 I 3000 Bsm I 1353
Sty I 1369
or

PflM I 1364
i

AlωN I 2884 Aυa I-BsoB I 1425

PpuM I 1438
BclV I 2682 Msc I 1444
Drd I 2575 Btg I 1447
rop PpuM I 1480
Afl III-Pci I 2473
BsrB I 2404 Bpu10 I 1580
Ear I 2351 Xmn I 2029 Bsg I 1650
Sap I 2350 Pυu II 2064 BspE I 1664
Nde I 2295 BsmB I 2122 BsaB I 1668
BstAP I 2291 Drd I 2162
BstZ17 I - Acc I 2244
BsaA I 2225 PfIFI - Tth111 I 2217

Figure 1 A physical map of plasmid pBR322 (via New England Biolabs).

Brenner’s Encyclopedia of Genetics, 2nd edition, Volume 5 doi:10.1016/B978-0-12-374984-0.01129-3 249