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Check Respiration in Plants Notes for NEET 2019 exam! In medical exams like NEET,
AIIMS, and JIPMER there are many questions asked from the Plant Physiology. This is one
of the important and Conceptual Unit. Here we are sharing detailed notes on Glycolysis,
Fermentation, Kerb’s Cycle etc. So, Let's begin with a brief introduction of Respiration
in Plants.
Respiration in Plants
Plant respiration is the biochemical process in which plant cells use sugars, the by-product
of photosynthesis, and oxygen to generate the energy required for growth and
development of the plant. Several enzymes catalyse the process and glycolysis is the first
step of respiration. The process of respiration can be explained by the following reaction:
The complete combustion of glucose generates carbon dioxide and water as the end
products and energy is released in the form of heat. Respiration can be either of aerobic
or anaerobic type. The difference between aerobic and anaerobic respiration is listed
below.
• Table showing the difference between aerobic and anaerobic respiration

is shown below:
Aerobic respiration Anaerobic respiration

Majority of organisms respire aerobically. It occurs in few organisms such as yeast.
Oxygen is used and generate CO2 always. Oxygen is not required and it may or may
not release CO2.
Glucose is fully oxidized and generates Glucose is not fully oxidized and thus
entire energy available in glucose. release only 5% of energy available in
glucose.
It involves five steps; glycolysis, pyruvate It involves only two steps; glycolysis and
oxidation, TCA (Tricarboxylic acid) cycle, incomplete breakdown of pyruvate.
ETS (electron transport system), and
chemiosmotic synthesis of ATP (adenosine
triphosphate).
It takes place in mitochondria and It takes place in cytoplasm only.
cytoplasm.
o In plants, respiration occurs in roots, stems, and leaves. Oxygen present in the
interspaces of soil gets diffused into the root cell through root hairs. Air gets
diffused through stomata in leaves and stem. In the case of the woody stem, the
gaseous exchange is carried out through lenticels.
Glycolysis
The Term glycolysis is derived from two Greek words; glycos meaning sugar and lysis
meaning splitting. Otto Mayerhof, J. Parsons, and Gustav gave the scheme of glycolysis.
It is a metabolic pathway in which glucose is converted into pyruvate and it occurs in the
cytoplasm.
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The several steps of glycolysis are illustrated in the figure. It consists of ten enzyme-
catalyzed reactions occurring in sequence. The metabolic pathway can be separated into
two phases:
1. Preparatory phase; at which point ATP is used
2. Pay off phase; at which point ATP is produced
Preparatory phase:
The first five steps of glycolysis are considered to be a preparatory phase. This phase
consumes energy to convert glucose into two three-carbon sugar phosphates.
• Glucose is phosphorylated to form glucose 6-phosphate (G6P) and the reaction
is catalyzed by enzymes called Hexokinases. This reaction consumes energy in
form of ATP.
• G6P is then acted upon by glucose phosphate isomerase and isomerized into
fructose 6-phosphate (F6P). It is a reversible reaction. Low F6P concentration
favours forward reaction and high F6P concentration favours reverse reaction.
• F6P is then converted to Fructose 1, 6-bisphosphate (F1-6-BP) and the
reaction is catalyzed by Phosphofructokinase. The reaction is coupled with
hydrolysis and is an irreversible reaction. This step is considered the key regulatory
point and also the rate-limiting step.
• F1-6-BP is then split into two triose sugars; GADP (Glyceraldehyde 3-
phosphate, an aldolase) and DHAP (Dihydroxyacetone phosphate, a ketose).
This reaction is catalyzed by fructose-bisphosphate aldolase.
• This step includes interconversion of DHAP with GADP, which then may proceed
further into glycolysis. This step simplifies the regulation as it directs DHAP down
the same pathway as GADP.
Pay off phase
There is a net gain of energy-rich molecules; NADH and ATP. The pay-off phase occurs
twice per glucose molecules as a glucose molecule results into two triose sugars in the
preparatory phase. Hence, it yields two NADH and two ATP molecules per glucose
from the glycolytic pathway.
• It involves oxidation of aldehyde groups of the triose sugars and there is an addition
of inorganic phosphate resulting into formation of 1,3-bisphosphoglycerate
(1,3BPG).
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• There is an enzymatic transfer of phosphate group from 1,3BPG to ADP to form 3-

phosphoglycerate (3PG) and ATP. It is catalysed by phosphoglycerate kinase. This
step is considered a substrate-level phosphorylation step.
• 3PG is then isomerized into 2-phosphoglycerate (2PG) by phosphoglycerate
mutase.
• 2PG is then converted into phosphoenolpyruvate (PEP) by enolase.
• PEP is then converted to a molecule of pyruvate and a molecule of ATP and the
reaction is catalysed by pyruvate kinase. This step is also considered as substrate-
level phosphorylation and also serves as an additional regulatory step.
The end product, Pyruvate, then enters into the matrix of mitochondria and undergoes
oxidative decarboxylation and yields acetyl CoA and NADH. Acetyl CoA then enters
into a cyclic pathway called the Tricarboxylic acid cycle or the Kreb’s cycle.
• Tricarboxylic acid Cycle: It is the second stage of cellular respiration. It involves
a series of chemical reactions where catabolism of organic fuel molecules such as
glucose and sugar takes place and stored energy is released in the form of ATP and
carbon dioxide.
• Electron Transport System and Oxidative phosphorylation: Electrons pass
from one carrier to another in ETS and occur in the inner mitochondrial membrane.
NADH and FADH2 from Kreb's cycle carry electrons to ETS and oxygen enters the
pathway as electron acceptor at the end of ETS.
Fermentation
Fermentation is the process in which there is incomplete oxidation of glucose.
o It takes place under anaerobic conditions.
o Fermentation, in the same manner as cellular respiration, start off with glycolysis.
o However, in the case of fermentation, pyruvate generated from glycolysis does not
undergo the consequent steps of respiration, namely oxidation, citric acid cycle,
and the electron transport chain.
o As fermentation does not include the electron transport chain, it is not considered
as a type of respiration.
There are two types of fermentation; Lactic acid fermentation and Alcohol fermentation.
i. Lactic acid fermentation: It occurs in fungi, bacteria, and muscle cells of the
animal. In the process, pyruvate molecules generated from glycolysis get reduced
to lactate and there is oxidation of NADH to NAD +. NAD+ is then replenished and
cycles back through glycolysis.
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ii. Alcohol fermentation: It occurs in yeast and bacteria. In this process, pyruvate gets
reduced to ethanol and along the process generates two molecules of carbon dioxide.
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• Table showing the difference between aerobic and anaerobic respiration

Aerobic respiration Anaerobic respiration

• There is an enzymatic transfer of phosphate group from 1,3BPG to ADP to form 3-

All about NEET examination: https://gradeup.co/medical-entrance-exams/neet

All the best!

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