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    DNA Experiment

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    Rico De Silva
    1. The student extracted DNA from human cheek epithelial cells and created a DNA bottle necklace. 2. The procedure followed a DNA extraction protocol which involved lysing the cells, adding protease to remove proteins, and precipitating the DNA with ethanol. 3. The student was successfully able to extract a small amount of visible DNA, though it was in low quantity likely due to excessive decantation during the procedure. The student then used decorations to create an aesthetically pleasing DNA bottle necklace.

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    DNA Experiment

    Uploaded by

    Rico De Silva
    250 views3 pages
    1. The student extracted DNA from human cheek epithelial cells and created a DNA bottle necklace. 2. The procedure followed a DNA extraction protocol which involved lysing the cells, adding protease to remove proteins, and precipitating the DNA with ethanol. 3. The student was successfully able to extract a small amount of visible DNA, though it was in low quantity likely due to excessive decantation during the procedure. The student then used decorations to create an aesthetically pleasing DNA bottle necklace.

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    DNA in a bottle

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    DNA experiment

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    1. The student extracted DNA from human cheek epithelial cells and created a DNA bottle necklace. 2. The procedure followed a DNA extraction protocol which involved lysing the cells, adding protease to remove proteins, and precipitating the DNA with ethanol. 3. The student was successfully able to extract a small amount of visible DNA, though it was in low quantity likely due to excessive decantation during the procedure. The student then used decorations to create an aesthetically pleasing DNA bottle necklace.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    250 views3 pages

    DNA Experiment

    Uploaded by

    Rico De Silva
    1. The student extracted DNA from human cheek epithelial cells and created a DNA bottle necklace. 2. The procedure followed a DNA extraction protocol which involved lysing the cells, adding protease to remove proteins, and precipitating the DNA with ethanol. 3. The student was successfully able to extract a small amount of visible DNA, though it was in low quantity likely due to excessive decantation during the procedure. The student then used decorations to create an aesthetically pleasing DNA bottle necklace.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    CN and Name: 3-De Silva Date Submitted: March 8, 2019

    Year and Section: 12A Lab Report #: 3

    DNA Extraction of Human Cheek Epithelial Cells and Creation of DNA in a


    Bottle necklace
    Background researchers for 30 minutes and to be spit back to
    DNA extraction is the process of the test tube. Afterwards, 2 mL of lysis buffer
    isolating DNA from a sample. DNA Extraction was obtained and was placed in the test tube.
    was first accomplished by Friedrich Miescher in
    1869. Through extracting DNA from
    lymphocytes, he was able to discover Nuclein
    (now called Nucleic acids). Today, DNA
    extraction has many applications like in routine
    diagnostic and theraputic decision-making. This
    is also used in discovering causes of diseases
    that involves genetics and also in developing
    new drugs (What is Biotechnology, 2019)

    The cells where the DNA will be


    extracted from would be the Human Cheek
    Epithelial Cells. These cells are found on the
    basal mucosa, a tissue that fixes the inside of the Figure I: Test tube with the Lysis Buffer and the
    mouth. These cells are known to secrete Mucin, Cheek Cells Sample
    an important part of mucous. These cells will be
    used because they were easy to extract as they The tube was to be inverted five times slowly.
    are always being removed from the body ( Afterwards, 5 mL of protease was placed inside
    Molecular Expressions, 2015) the test tube. The test tube was to be inverted
    few times. The test tube was then placed in the
    Objective/s water bath with a temperature of 50 degrees
    The investigation aims to extract DNA celsius for 10 minutes.
    from Human Cheek Epithelial Cells. There were
    no independent variables nor dependent
    variables because there was only one
    experimental group. What was kept controlled
    throughout the duration of the experiment would
    be the protocol used and the materials used in
    the experiment.

    Procedure
    The procedures followed the protocol
    given by Bio-Rad. The experiment started with
    the extraction and precipitation of DNA. A 15
    mL test tube was filled with 3 mL of drinkable
    water. The researchers were then required to
    mildly chew their cheeks. The water from the 15
    Figure II: Test tube with the Cheek Cells and the
    mL test tube was to be gargled by the Water Bath to be used
    It could be seen in Figure IV that the DNA
    was successfully extracted into the small bottle
    provided by the DNA extraction kit.

    Analysis and Conclusion


    The experiment involved addition of
    many different components to the sample.The
    function of lysis buffer breaks open the cell
    membrane of the cells as said by Brennan
    (2018). The addition of protease was a way to
    remove the remaining proteins, enzymes and
    nucleases in the sample (The Protein Man,
    2013). The last process was called Ethanol
    precipitation. The function of it was to make the
    Figure III: Sample Being Placed in a Water Bath for
    DNA less hydrophilic and to make the DNA
    10 Minutes
    more visible (Oswald, 2018).
    Afterwards, 10 mL of 95% ethanol was placed
    in the test tube. The DNA was to be left It could be concluded that this DNA
    undisturbed for five minutes. The test tube was extraction was successful. The DNA is visible in
    then inverted five times. Lastly, the DNA was the bottle. However, the DNA was only in small
    extracted using a pipette. amount. This is most likely caused by excessive
    decantation.
    Students afterwards were given
    materials to make their own DNA in a bottle. Bibliography
    Students were given the option to bring Brennan, J. (2018, May 21). Components of
    decorations in order to make the necklace look Lysis Buffers. Retrieved March 7, 2019,
    more aesthetically pleasing. from
    Data/Observations https://sciencing.com/components-lysis-
    buffers-8148370.html
    Molecular Expressions. (2015, November 13).
    Human Cheek Epithelial Cells.
    Retrieved March 2, 2019, from
    https://micro.magnet.fsu.edu/primer/tech
    niques/dic/dicgallery/cheekcellssmall.ht
    ml
    Oswald, N. (2018, June 27). Ethanol
    Precipitation of DNA and RNA: How it
    works. Retrieved March 7, 2019, from
    https://bitesizebio.com/253/the-basics-h
    ow-ethanol-precipitation-of-dna-and-rna
    -works/
    The Protein Man. (2013, April 17). A
    Discussion of Protein Research.
    Retrieved March 7, 2019, from
    https://info.gbiosciences.com/blog/bid/1
    77310/Understanding-the-Role-of-Prote
    Figure IV: End Result of the Laboratory Experiment. ases-in-DNA-Extraction
    What is Biotechnology. (2019). DNA extraction
    isolates DNA from biological material.
    Retrieved March 2, 2019, from
    http://www.whatisbiotechnology.org/ind
    ex.php/science/summary/extraction/dna-
    extraction-isolates-dna-from-biological-
    material

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