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The document summarizes optimization work for preparing a graphene/carbon cloth anode for microbial fuel cells. Various characterization techniques were used to determine that electrophoretic deposition of graphene oxide for 10 minutes led to fully coated graphene on the carbon fiber surface, exhibiting optimal electrochemical performance. The graphene/carbon cloth anode showed higher power generation and mediated microbial metabolism compared to a plain carbon cloth anode.

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Shreyansh Garhewal
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51 views9 pages

Adams and Adams

The document summarizes optimization work for preparing a graphene/carbon cloth anode for microbial fuel cells. Various characterization techniques were used to determine that electrophoretic deposition of graphene oxide for 10 minutes led to fully coated graphene on the carbon fiber surface, exhibiting optimal electrochemical performance. The graphene/carbon cloth anode showed higher power generation and mediated microbial metabolism compared to a plain carbon cloth anode.

Uploaded by

Shreyansh Garhewal
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Supporting Information Available

Graphene/carbon cloth anode for high-performance

mediatorless microbial fuel cells

Jing Liu, Yan Qiao, Chun Xian Guo, Sierin Lim, Hao Song, Chang Ming Li*

Optimization work of graphene/carbon cloth anode preparation

Electrophoretic deposition of GO on the carbon cloth (E-TEK, B1D, plain, with

geometric area of 2.0 cm2) was conducted in 0.2 mg/ml GO solution by using 0.3

mA/cm2 anodic current in a two-electrode system with Pt sheet as the counter

electrode followed by a 1.2 mA/cm 2 cathodic current. The electrophoretic deposition

time was optimized by using 1, 2, 4, 6, 8, 10, 12, 14, 16 min. CVs and EIS

measurements of so-prepared graphene/carbon cloth anodes were tested in potassium

hexacyanoferrate (10 mM K3[Fe(CN)6] + 1M KCl) and the results are shown in Fig.

S1. As electrodeposition time increased, the peak separation of CVs (Fig. S1a) and

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charge transfer resistance (Fig. S1b) decreased until deposition time reached 10 min

and then they remained almost stable, indicating that at this time point the graphene is

fully coated on the surface carbon fiber.

  

Figure S1. CVs (a) and EIS (b) measurements of graphene/carbon cloth anodes 

prepared by different electrodeposition time, the inset of Fig. S1b is charge transfer

resistance under different electrodeposition time.

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Figure  S2.  Raman  spectra (a), and FESEM  images  of plain carbon cloth  (b) and

graphene/carbon cloth (c).

Figure  S3.  Discharge   performance   of   one   graphene/carbon   electrode   in   two

consecutively­setup MFCs. When the output current density of the first MFC (MFC­

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A) dropped sharply, the graphene/carbon electrode was collected and used as anode in

a   newly­setup   MFC   (MFC­B),   in   which   the   graphene/carbon   electrode   could   still

deliver the current density as high as that in the first MFC. This result indicates that

the stability of graphene/carbon electrode is very good, and can be repeatedly used.

Figure S4. Control CV experiments with carbon cloth electrode in glucose-free

medium without bacteria cells.

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Figure  S5.  FESEM images of  P. aeruginosa­adhered  plain carbon cloth anode (a),

and graphene/carbon cloth anode (b).

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Figure S6. UV­Vis spectra of anodic supernatant for carbon cloth anode based MFC

(MFC/cloth) and graphene/carbon cloth anode based MFC (MFC/graphene)

FTIR spectrum analysis

In FTIR spectrum (Fig. S2a) the strong band at 1600 cm-1 indicates the stretch

vibration of the C=C and C=N in the polycyclic aromatic ring. The broad strong peak

at 3400 cm-1 should be assigned to –OH stretching. There is no C=O bond contained

in the compound as no stretch vibration peak showed around 1700 cm -1. The

characteristic bands locate over the fingerprint range of 1400-400 cm -1. For

comparison, the FTIR spectrum of pure PYO (Sigma) treated with culture medium

was recorded and shows similar bands with that of the anodic supernatant (Fig. S2b).

Figure S7. FTIR transmittance spectra of anodic electrolyte (a) and PYO (b).

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Standard curve of PYO

PYO powder (Sigma) was dissolved in DI water to a series concentration (from 0-

10 μg/ml) and subjected to UV-Vis spectrum analysis. The standard curve was draw

according to the absorption at 311 nm, at which wavelength the PYO shows a strong

absorption peak.

Figure S8. UV-Vis spectra of PYO, the inset is standard curve of PYO according to

absorbance at 311 nm.

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Figure S9. Proliferation curves of bacteria in anodic chamber of MFC during

discharge.

Glucose assay

Glucose concentration of anodic supernatant was measured using glucose assay kit

(Sigma). A series of standard glucose solution was prepared (20μg/ml, 40μg/ml,

60μg/ml, 80μg/ml), both standard solution and test solution was processed according

to the protocol. The absorbance at 540 nm and the standard curve is shown below, and

the glucose concentration in the anodic chamber was calculated (Table S1).

Table S1. Absorbance of reactant at 540nm.

Sample S0 S1 S2 S3 S4 MFC/cloth MFC/graphene


Concentration 0 20 40 60 80 0.56 0.56

(μg/ml)
Absorbance 0.154 0.302 0.445 0.601 0.006 0.006

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Figure S10. Standard curve of glucose concentration.

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