The document outlines the methodology for propagating and cultivating Bacillus thuringiensis aizawai, including 8 steps: 1) preparing propagation media, 2) performing wet cultivation in erlenmeyer flasks with various nutrients, 3) dry cultivation using onggok and tofu liquid waste, 4) testing the pH of samples, 5) testing optical density, 6) measuring dry biomass by centrifuging and drying samples, 7) performing a viable spore count test by heating samples and culturing on agar plates.
The document outlines the methodology for propagating and cultivating Bacillus thuringiensis aizawai, including 8 steps: 1) preparing propagation media, 2) performing wet cultivation in erlenmeyer flasks with various nutrients, 3) dry cultivation using onggok and tofu liquid waste, 4) testing the pH of samples, 5) testing optical density, 6) measuring dry biomass by centrifuging and drying samples, 7) performing a viable spore count test by heating samples and culturing on agar plates.
The document outlines the methodology for propagating and cultivating Bacillus thuringiensis aizawai, including 8 steps: 1) preparing propagation media, 2) performing wet cultivation in erlenmeyer flasks with various nutrients, 3) dry cultivation using onggok and tofu liquid waste, 4) testing the pH of samples, 5) testing optical density, 6) measuring dry biomass by centrifuging and drying samples, 7) performing a viable spore count test by heating samples and culturing on agar plates.
The document outlines the methodology for propagating and cultivating Bacillus thuringiensis aizawai, including 8 steps: 1) preparing propagation media, 2) performing wet cultivation in erlenmeyer flasks with various nutrients, 3) dry cultivation using onggok and tofu liquid waste, 4) testing the pH of samples, 5) testing optical density, 6) measuring dry biomass by centrifuging and drying samples, 7) performing a viable spore count test by heating samples and culturing on agar plates.
