SPECIMEN PREPARATION
Arevalo, Balbis, Beronical, G. Buenaventura,
Castillo, Gajisan, Labsan
A sample for medical testing
Crucial in clinical management decisions and
provide important prognostic data
Nodal in a patient’s illness; shapes the choice
of relevant medical and surgical therapies and
determining follow-up routines
To identify the criteria for rejection of
specimen
To identify the different materials used for
gross examination of specimens
To familiarize with the general concepts of
describing specimens
To identify the concepts and general
procedures of sectioning the most common
surgical specimens
To create a specimen worksheet
Unlabelled specimens
Mislabelled specimens
Incorrect container/ wrong preservative used
Insufficient specimen for procedure
Unsuitable specimen for procedure
Cutting tools/ Standard dissecting kits
Specimen container
Labelling materials
Tissue cassettes
Properly examine the specimen, the
container, and the label
It must have the following :
◦ Patient Name - First and last (no nicknames or
abbreviations
◦ Secondary Identifier - Medical record number, date
of birth, or other unique identifier
◦ Date and time of collection
◦ Initials of collector
Follow the criteria for rejection of a specimen
1. Prepare the specimen in a preferred
uncontaminated area
2. Measure the dimensions
3. Weigh the specimen (round off to the
nearest 0.1g)
4. Describe the appearance of the specimen
Measurements in :
Length
Width
Diameter
Weight
Appearance based on:
◦ Color
◦ Consistency
Dissect the specimen into 1.0 cm thick
sections
Specimens should be squared
◦ Place specimens into tissue cassettes . Must fit the
cassettes (not more than 0.3 cm in thickness)
◦ Cassettes must not bulge
Label the cassettes
Always wear PPE
Maintain cleanliness in the lab
Always clean a microtome blade before and
after use
Never throw the specimens and their
respective container until it is already cleared
for disposal
Always note the preservatives used and
observe proper maintenance (temp, pH, etc.)