User Manual

(HDCE-30C Digital Camera)

2008.09
 Contents

1. Introduction .......................................................................................................................................... 2
Specification....................................................................................................................................... 2
Packaging Box Contents..................................................................................................................... 2
2. Installation Instruction ........................................................................................................................ 4
2.1 Minimum System Requirements .................................................................................................. 4
2.2 Common sense about hardware and software .............................................................................. 4
2.3 Install Introduction for Windows XP............................................................................................ 5
2.4 Attention in Windows Vista and Windows 2000 .......................................................................... 7
2.5 Check whether the device is rightly installed ............................................................................... 9
2.6 Uninstall ..................................................................................................................................... 10
3. How to update the driver ................................................................................................................... 10
4. Camera Using Guide .......................................................................................................................... 16
4.1 Attention.................................................................................................................................... 17
4.2 Introduce of DirectShow Technique ........................................................................................... 17
4.3 Introduce of Twain Capture Technique....................................................................................... 18
5. Use the Camera in ScopeImage Plus................................................................................................. 18
5.1 An overview of ScopeImage Plus............................................................................................... 18
5.2 Quick learn to use ScopeImage Plus .......................................................................................... 19
5.3 Video Preview and Capture ........................................................................................................ 21
5.3.1 Use Directshow to Preview ............................................................................................ 21
(a) Start your Camera ....................................................................................................... 21
(b) View the Video in Full Screen Format ........................................................................ 22
(c) Set the Preview size .................................................................................................... 22
(d) perform Auto White Balance (AWB) and Auto Exposure (AE) ................................. 23
5.3.2 Use Twain to Preview:.................................................................................................... 25
5.4 Layer and Draw .......................................................................................................................... 26
5.4.1 Use the Image Processing Tool Box (Layer and Draw) ................................................. 26
5.4.2 Software Power Setup (Calibration)............................................................................... 34
5.4.3 Video Watermark ............................................................................................................ 41
5.5 Image Processing........................................................................................................................ 44
5.6 Image Browser............................................................................................................................ 44
5.7 Help Contents ............................................................................................................................. 45
6. Use the Camera in ScopeImage Advanced ....................................................................................... 47
6.1 An overview of the ScopeImage Advanced................................................................................ 47
6.2 Use Directshow to Preview in ScopeImage Advanced............................................................... 47
(a) Open the Directshow Preview Window ............................................................................. 47
(b) Adjust the Color. ................................................................................................................ 48
(c) Do the Calibrating in ScopeImage Advanced. ................................................................... 49
6.3 Use Twain to Preview in ScopeImage Advanced ....................................................................... 51
7. Trouble shooting................................................................................................................................. 52
Never disassembly the instruments yourself! ................................................................................... 52
USB Device conflict......................................................................................................................... 52
Image Morphing ............................................................................................................................... 52
Application fails to start ................................................................................................................... 53

1
Thank you for purchasing our microscope product. To avoid any misuse and
damage, please read the operation instruction carefully before operation.
This is a color USB2.0 digital image system that captures microscope images and
displays real-time live video on your PC screen. It comes with a high-resolution
digital camera, built-in reduction lens, user-friendly software, and adapters for all
sizes of microscopes.

1. Introduction
Specification
Parameter Typical Value
Optical format 1/2-inch (4:3)
Active imager size 6.55mm(H) × 4.92mm(V) 8.19 (Diagonal)
Active pixels 2048H × 1536V
Pixel size 3.2μm × 3.2μm
Global reset release (GRR), electronic rolling
Shutter type
shutter (ERS)
Image Format Frame rate 6 fps @ 2048×1536
Sensitivity >1.0 V/lux-sec (550nm)
Dynamic range 61dB
SNR 43dB
Operating temperature 0°C ~ 60°C
Image Output USB2.0, Directshow and Twain
Operating System Windows 2000/XP/Vista
·With High Resolution chip(3.0M),Providing High Quality Image and Widely Used in Academic and
Medical Field for High Precision and Resolution Image Capturing and Processing;
·Very convenient to Use, just insert it into Eyepiece Tube or Top Tube of Trinocular Head;
·By USB2.0, getting Real-time and Non-compressing Video Data and Capturing Image Directly;

Packaging Box Contents

The HDCE-30C camera packaging box includes:
1. The HDCE-30C Camera device with a High-Quality IR Cutoff Filter inside.
2. High Quality USB2.0 Cable
2. 30mm adaptor (Optional: 30.5mm adaptor)
3. NDPL-10: 0.45X medium adapter
4. CD: Software includes the ‘ScopeImage Plus’ and ‘ScopeImage Advanced’ software. The Driver
contains hardware driver, TWAIN DS and DirectShow Source Filter. Additional information
and manuals/documents can be found on the CD.

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Optional (choose to buy): 0.01mm or 0.1mm Calibration Slide.

Please assemble the accessories as follows:

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2. Installation Instruction

2.1 Minimum System Requirements

System Requirements
- Operating System Windows 2000, XP or Windows Vista
- DirectX 9.0 or higher (for Windows 2000)
- Video adapter supports 24bit color or more and 1280*1024 or 1024*768 resolution
- CPU with 2.0GHz or more
- System Memory 256MB or more, Display Memory 128MB or more
- USB2.0 interface
- Hard Disk Space 1GB for installation plus additional space for captured images
Since video processing is hardware intensive, a faster computer with a fast
hard disk drive and extra memory will yield better results.

2.2 Common sense about hardware and software

Here introduce the basic sense of digital camera. Generally speaking, it includes two sections:
one is the hardware driver; the other is the software application.
The driver is combining with the hardware (the chip of the digital camera) closely. The
computer can operate the hardware, only when it has installed the right driver. Say in brief, it’s
the question of can and can not.
The application, as we know the ScopeImage Plus, ScopeImage Advanced and so on.
These applications perform the task that the users need; it’s the
question of how to operate.

Connect the Camera to the Computer

Connect USB devices to a computer while the computer is turned
on. This “hot plug” capability is a convenient feature of USB
products. To connect the camera to a computer, do as follows:
Plug one end of the USB cable to an open USB port on your
computer, and the other end to the camera. When you connect it
rightly, the computer’s Operating System should recognize it, and
pops up a found new hardware dialog. The camera’s RED LED
should on. Please follow the clue to install the hardware and
software of the camera device.

Attention:
Please use the same USB2.0 port; otherwise you need to update your driver again to
make the camera work normally. And refer to the chapter 3 to update the driver.

Before installation
Follow the CD to setup the hardware and software of the Camera, and the image processing
software ‘ScopeImage Plus’. Please link the digital microscope to the computer with the
USB2.0 cable before you run the setup.

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There’re a few differences among the camera installation in Windows XP, Vista and 2000, here
shows the introduction in Windows XP for an example.

2.3 Install Introduction for Windows XP

1. The system finds new hardware, and comes out New Hardware Wizard dialog. Please click
“Cancel”.

2. Double click the ‘HDCE-30C Setup (En).exe’ in the CD. The wizard will lead you to install both
the hardware and software, after the installation, you can use the camera. Please follow the clue
to install it.

3. Click ‘next’.

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 4. Click Install

5. Software Installation. Just click ‘Continue Anyway’, Windows will continue the installation.

6. Hardware Installation. When your camera is linked, there will come out the dialog as above, just click
‘Continue Anyway’ to continue the installation.

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7. Attention: If you have not attached your camera to your computer before the installation,
there will come out a dialog telling you to attach your device. Click ‘OK’

8. Click ‘Finish’, installation has been completed.

If there comes out the error dialog when you launch the application

Please Install the under the folder ‘ActiveX’.

You’d better restart your computer to make the new device work rightly.

2.4 Attention in Windows Vista and Windows 2000

In the Windows Vista System, there will come out a warring dialog telling ‘An unidentified

program wants access to your computer’, please clicks ‘Allow’. And at the windows security dialog,
please click ‘Install this driver software Anyway’.

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In the Windows 2000 System, you need to install DirectX9.0 to obtain better picture

quality. Double click the document dxsetup.exe in the folder “DirectX9.0”, run the DirectX
installation

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You’d better restart your computer to make the new device work rightly. And you can check it bellow
the “Imaging Devices” in “Device Manager / Hardware”. Then you can use the camera to
preview the images.

2.5 Check whether the device is rightly installed

(1). After the installation, there will be a quick launch icon on the desktop, as follows:

(2). Check the Start->ScopeImage Plus as Fig 2.4.1

Fig 2.4.1
(3). Check the hardware driver, whether there an “Imaging Devices” Camera-H3C in
“Device Manager / Hardware”, as Fig 2.4.2 (Make sure the camera is linked with the
computer, and the camera’s red LED is on, when you check the hardware driver.)

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 Fig 2.4.2

Note: If there’s no imaging device in the device manager, or there’s a yellow warning
symbol before the imaging device “Camera-H3C”, it means you have not installed the
hardware device rightly, and you need to update the driver, please refer to the chapter “3
How to update the driver” to reinstall the driver.

2.6 Uninstall
Click Start->Program->Camera-H3C->Uninstall.exe, and follow the clue to uninstall it.

Click “yes”, the hardware and software will both be uninstalled.

3. How to update the driver

If the camera does not work, or it conflict with other hardware of your computer, you need
to update the driver. (Make sure you have run the setup before.) Or you can also uninstall it
first, and then reinstall it again.
There are two ways to update the driver, please do as follows:
1, Plug and unplug the camera, or try another USB2.0 port, then the system will
comes out found new hardware wizard.
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2, Go to Control Panel, as follows:

(1) Access Windows Control Panel. Double click ‘System’

(2) Select Device Manager.

(3) Click ‘Imaging devices’

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 (4) Right click ‘USB Device’, click ‘Update Driver…’, comes out found new hardware
wizard.

1. Choose “this time only”, click next.

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There are two ways for you to update your driver, you can choose ‘Install the software
automatically’, and then click next; or you can also choose ‘Install from a list or
specific location (Advanced), then click next. Generally, we advise you to choose the
first choice.
1) If you choose ‘Install the software automatically’, click next.

2) The system will find the right driver for the camera by itself, if find, there will come out a warning
dialog, please choose ‘Continue Anyway’.

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3) Click ‘Finish’.

Hardware install has been finished; you can see the “Camera-H3C” bellow “Imaging Devices” in
“Device Manager / Hardware”.

Sometimes the system can not find the driver by itself and the driver update is failure;
you can redo it by choosing the second choice ‘Install from a list or specific location
(Advanced)’, then click next.

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2. Choose ‘Install from a list or specific location (Advanced)’, click next.

3. Choose ‘Don’t search. I will choose the driver to install.’

4. Choose ‘Camera-H3C’, Click ‘Next’.

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5. Hardware Installation, click ‘Continue Anyway’.

1. Click ‘Finish’. Hardware installation has been finished.

You’d better restart your computer to make the new device work rightly. And you can check it bellow
the “Imaging Devices” in “Device Manager / Hardware”. Then you can use the camera to
preview the images.

In Windows Vista system, you need to uninstall the driver clearly, and

then install the recent version driver and software.

4. Camera Using Guide

Our camera includes two powerful software ScopeImage Plus and ScopeImage
Advanced, it is extremely convenient to do the basic and advanced image processing and
measurement to the images captured. The simple operation ensures you doing all these
processing works at great ease.

Here we list the points for your special attention before using the camera.

Our camera supports both directshow and twain interface, and we still make a simple
introduction of the directshow technique and twain technique, and shows you the sample
use of the camera in ScopeImage Plus and ScopeImage Advanced. You can refer to the
‘help’ menu of the ScopeImage Plus and the ‘User Manual.pdf’ of ScopeImage Advanced
16
to get more information about how to use the software.

4.1 Attention
★The manufacturing and assembling of the device need to be in the dust-free
environment. Please do not attempt to open the camera case. There are no
user serviceable parts inside. If you have any concerns about the device
quality or the operation, please contact the dealer with detailed
information.
★The camera comes with two different software applications; You are not
able to use both of the applications at the same time since they are using
the same device (For example Camera-H1A). You need to close one
application to open anther one, otherwise they won’t work properly. For the
same reason, you also can not use the directshow and twain (two different
driver for the camera, see the instruction for details) at the same time.
★Please use the same USB2.0 port every time. Using on a different USB2.0
port may require to updating your driver again to make the camera work.
Refer to the chapter 3 to update the driver.
★USB2.0 is a mandatory, not optional. This camera cannot work on the
computers with USB1.1 ports.
★ScopeImage Advanced, one of the two included applications, is locked for
unauthorized use. A special USB jump drive (also called USB Dog) is
supplied to unlock this application. To use ScopeImage Advanced, you
only need to plug the USB jump drive (USB Dog) into any of your USB2.0
ports.
★Please unplug the USB cable from the computer immediately after use. It
will shorten the lifetime of the camera, sometimes may cause repair service
if the camera is plugged in the computer all the time and you do not shut
down the computer for a long time.

4.2 Introduce of DirectShow Technique

DirectShow is designed to address each of these challenges. Its main design goal is to
simplify the task of creating multimedia applications on the Windows® platform by isolating
applications from the complexities of data transports, hardware differences, and
synchronization issues.
To achieve the throughput necessary for streaming video and audio, DirectShow uses
DirectDraw® and DirectSound® to render data efficiently to the system's sound and
graphics cards. Synchronization is achieved by encapsulating the multimedia data in
time-stamped media samples. To handle the variety of sources, formats, and hardware
devices, DirectShow uses a modular architecture in which operating system components
called filters can be mixed and matched to provide support for many different scenarios.
DirectShow includes filters that support for codec written for the Audio Compression
Manager (ACM) and Video Compression Manager (VCM) interfaces.
DirectShow enables applications to play files and streams from various sources,
including local files and remote files on a network. DirectShow can compress and
decompress for some file formats, and many third-party hardware and software decoders
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are compatible with DirectShow. In addition, DirectShow supports legacy VFW codec
based on the Video Compression Manager (VCM) and Audio Compression Manager (ACM)
interfaces. Playback makes full use of DirectDraw hardware acceleration and DirectSound
capabilities when the hardware supports it.

4.3 Introduce of Twain Capture Technique

TWAIN Interface is a cross-platform interface for acquiring images captured by certain
scanners, digital cameras, and frame grabbers. The manufacturer of the TWAIN device
must provide a Source Manager and TWAIN Data source for your device to work with
ScopeImage Plus and ScopeImage Advanced.
You must install the TWAIN device and its software, and restart your computer, before
you can use it to import images into ScopeImage Plus and ScopeImage Advanced.
If more than one TWAIN device is installed in your system and you want to switch
devices, use the TWAIN Select Device command

(Note: When you run the installation, the twain device and software, the twain has been
installed with the software already.)

5. Use the Camera in ScopeImage Plus

5.1 An overview of ScopeImage Plus
ScopeImage Plus is the image-processing software professionally designed for
HDCE Series digital camera. It allows you to view, capture, edit, record, count,
zoom, file, and manage still or moving microscope images. Specific features include
the following:
1) Image format: supports many file formats, especially the native format (*.sft)
preserve the most complete information for images acquired by ScopeImage Plus,
including sampling interval and magnification settings, annotations and so on.
2) Image preview: support DirectShow, VFW (Video for Window) and Twain
interface
3) Basic image operations like other similar software are included in the Image
menu. You can adjust the image at ease.
4) Image cut & paste from image to image, image to file.
5) ScopeImage Plus uses the layer technique to record the extra information
attached with the images, and the different results can be put on different layers,
Image layer operation specially designed for image processing and measurement.
6) Image processing filters for noise reduction and enhancement (Image
enhancement filters, edge enhancement filters, morphology operation filters. Also
user defined kernel filter, includes convolution and morphological filters).
7) Image arithmetic operation between image and image, image and digital
number (Including AND, OR, XOR, NAND, NOR, NOT, Add, Subtract, Multiply,
Divide, Absolute, Min, Max, Average, Invert, Logarithm, Exponential, Square Root,
Square, X to the power of Y operation)
8) Image pseudo color, range, segmentation operations are also include in the
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application
9) Image zooming operations and panning operations
10) Image browser, can let you efficiently find and organize your images.

5.2 Quick learn to use ScopeImage Plus

Quick Index
This is a quick index of ScopeImage Plus application. This index is designed for those who
wish to master the ScopeImage Plus operations in a short time.

1.To get the video from Twain Acquire (its shortcuts

are and on Image Window Toolbar ).

How to display the
2. To use DirectShow Capture Technique. Its shortcut is
video from the
.
video devices?
3. File->Import Image->DirectShow Capture....

4. File->Import Image->Twain Acquire....

1. Display the video using DirectShow Capture Technique

How to define the first.

Software Power? 2. According to Software Power Setup to define your own

Software Power step by step.

1. Define your own Software Power according to Software

Power Setup, otherwise, the unit will be Pixel only for the
How to perform the captured image.
measurement
2.Select the right Software Power from list
operation (we use
on DirectShow Capture Window Toolbar and let this Software
term Draw in the
Power equal to your real microscope objective lens power you
application)?
are using to capture the image .

3. Capture an image using button on the DirectShow

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 Capture Window Toolbar.

3. Make a new layer on the Background layer using

Draw->New menu or Tool Box Layer Page. The Draw Toolbar and
Draw menu will become active.

4. Select the draw icons on the Draw Toolbar or Draw menu to

do the draw (measurement) operations.

5. You can view and change the object Appearance and

Coordinates from Tool Box Draw Page by selecting one object .
You can also find the multiple objects parameters on the
layer using Draw Manager.... You can also print these
parameters with the image together. See Options->Preference...,
Click Print to set the print operation.

1. The only format to save the draw results is to use the

file extension *.sft. See Save As for detail.

How to save the 2. You can let the application always "Remind me when save
Draw results? file with draw items not in *.sft format" by check the term "Remind
me when save file with draw items not in *.sft format" in
Options->Preference...Misc dialog.

1.You can get the video from Twain Acquire (Its shortcuts

How to perform are and on Image Window Toolbar ).

Twain Acquire?
2. Choose File->Import Image->Twain Acquire...to display the Twain
video and then capture it.

There are many units for the length parameter. See

How to add the
Options->Draw... ->Unit->Length menu to select the right
length unit?
length unit.

How to define the The angle can be Radian, Degree and PI. See
angle unit? Options->Draw...->Unit->Angle dialog box to select the right

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 angle unit.

How to select the 1. Menu method: File->Image Import...->Software Power.

Software Power in
2. Icon method: Select the right Software Power from
the DirectShow
list on DirectShow Capture Window Toolbar .
Capture Window?

5.3 Video Preview and Capture

5.3.1 Use Directshow to Preview

(a) Start your Camera

1. Make sure the camera is linked with the computer with the USB2.0 wire, and your computer
is USB2.0 port, otherwise, the device will not run normally.
2. Run the ScopeImage Plus software you have installed.
3. Click ‘Camera-H3C’ button on the mainframe toolbar, or click the device on the start page
to start your camera, shows below:

You can also do it by the menu. Please select File->Import Image->Live

Capture->Camera-H3C.

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(b) View the Video in Full Screen Format

Three ways to view the video in full screen format:

(1) Double click your left mouse to go to full screen format, and double
click to exit
(2) Right click the right mouse, select ‘Full Screen’

(3) Press ESC will also enter the full screen preview operation, and press
ESC again to exit.

(c) Set the Preview size

To set the size of preview window, please select the menu ’Setup->Video
Stream Format…’ and the stream format property is as follows:

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Note:
The higher resolution, the lower frame rate will be. If you want to increase frame
rates to make the preview video see more fluently, you need to decrease the
resolution, such as 1024x768 or 800x600.

(d) perform Auto White Balance (AWB) and Auto Exposure (AE)

About white balance:

White balance basically means color balance. It is a function which gives the camera a
reference to "true white" — it tells the camera what the color white looks like, so the camera will
record it correctly. Since white light is the sum of all other colors, the camera will then display all
colors correctly.

Incorrect white balance shows up as pictures with orange or blue tints, as demonstrated by the
following examples:

Correct color balance Color balance too blue Color balance too red

Another very important parameter that affects the color of the images is the value of the exposure. Our
camera support both auto exposure and manual exposure.

About auto exposure:

23
It means the camera will adjust the exposure value by itself to make the images display in normal. When
the light source is too bright, the camera will decrease the exposure value, and when the light source is
too dim, the camera will increase the exposure value. It just adjusts the exposure value by itself.

Select Setup->Video Source Property… on the menu to open the Video Image Properties dialog.

Before auto white balance, please make sure to move your sample to
blank area (no sample pattern in the field).

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5.3.2 Use Twain to Preview:
1). Select the twain source

Click the button on the tools bar, , choose

Camera-H3C

2).Open the Twain source

Click the button on the tools bar.

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With this twain interface, you can also open the camera in any software that support this interface, such
as ACDSee, PhotoShop and so on.
Note: If you use just one type camera, you can skip step 1 (Select Twain Source) after you use the
camera for the first time.

5.4 Layer and Draw

5.4.1 Use the Image Processing Tool Box (Layer and Draw)

(a) Create a layer, and enable the draw tools bar

You may find the measurement tools are dim and you cannot click on them after you
capture a frame. The reason is that you are at the “background layer” and you
cannot do those measurements on the “background layer”. So you need to create a
new layer (still the same image). Here are the steps to activate the measurement
tools:
• Capture a frame

• Click “Tool Box” under “View” menu, or click button on the tools bar
• Click “Layer Manager”
• Right click the mouse on the white grid pane, then left click on “new”
• Enter a layer name and click “OK”
Now you are working on a new layer and all the measurement tools are enabled.
See the pictures below.

Open the Tool Box Create a new layer

26
 Name the new layer and the label color

A new layer created and the draw tool bar will be enabled

27
(b) Do angle measurement for an example

Here shows how to do the angle measurement for example:

Click the angle icon on the tools bar and do angle measurement.

(c) Change the draw object appearance (Single object)

28
 Modify angle appearance (include the line color, width and the line type)

Now angle appearance has been changed.

(d) Change the angle unit

Now the angle unit is radian; we can change the angle unit to ‘degree’ or ‘pi’; Click
the menu ‘Options->Draw…’ to open the draw property dialog.

29
 Before (Radian) After (Degree)

The angle unit has been changed from radian to degree.

The measurement result displays with the draw at the same time.
30
(e) Open the Draw Manager

Click the menu ‘View->Draw Manager’ to open the draw manager.

Show the draw object information

(f) Print the image with the draw and measurement result

Click the menu ‘Options->Preferences…’ to open the Printing Setup Dialog.

31
Print preference: choose the page usage to ‘Auto’ or ‘Don’t use another paper’.

Click the menu ‘File->Print Preview…’ to preview the images you want to print.

Print preview

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(g) Save the complete information with the image together (*.sft)

ScopeImage Plus supply the native format (*.sft) to preserve the complete information for
images, including sampling interval and magnification settings, annotations and so on.
Save the image files in ‘.sft’ format, click File->Save as… on the menu.

Select format (.sft format) to keep the draw information on the image.

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(h) Export the draw result

Export data to image (unrecoverable), save the image with the draw.

Export the draw result, three ways: to Clipboard, to Html File or to Excel

5.4.2 Software Power Setup (Calibration)

(Remember to set them up in the DirectShow )

If you have a microscope with 4X, 10X, 40X, 100X objective lens, we can say this
microscope have four different objective powers.

However, if this microscope is combined with different digital cameras when

capturing the images, the magnification of the images taken by the software is no longer
the same as the magnification of the objective lens. Instead, it is represented by the
resolution, which is determined by the Software Power of the whole system (microscope
plus digital camera).

For the consistency, we tried to build up a menu, called Software Power with some
submenus named 4X, 10X, 40X, 100X to denote the resolution. Therefore, these Software
powers are actually the system powers, corresponding to the image resolution.

When the Software Powers are calibrated, user can easily select the Software Powers
(in the software) to be the same as the microscope objective power, then after the image is
captured, the resolution can be saved with the image data together. If user wish to measure
the object dimensions on the image, he need not to care about the detailed resolution or in
34
which objective power the image is captured, the software will calculate the object
dimensions according to the resolution, the selected unit, automatically for further analysis.

So the first step is to set correct Software Power. Different microscope and digital
camera combination need to set their Software Powers specifically.

Now we begin to set the Software Power for the digital microscope (Note: we need to
have micro ruler with minimum increment 0.01mm or 10um, its overall length is 1mm. It
is recommended that the stage micrometer or scale used in the calibration should be
traceable to the National Institute of Standards and Technology (NIST) or a similar
organization.

Fig.1 The micro ruler for the calibration of the digital microscope

1. Plug in the electric eyepiece (or digital camera for microscope) into the trinocular,
and plug in the USB connector into the computer USB port (For USB2.0 camera, be sure
to plug it in to the right one, some computer only has one USB2.0 port), run ScopeImage
Plus

2. Select File-> Import Image->DirectShow Capture...(Or directly click the video icon

on the Image Window Toolbar , a video

window will be displayed, select Devices->Video Device Name (depend on the devices
installed in your computer). Click drop-down arrow button on the unit combo box

on the video window DirectShow Capture Window Toolbar to show

the Software Powers, because no power is added, the default term is Pixel, select
"Pixel" as the video unit, the window should look like:

35
 Fig.2 Video window of the ScopeImage Plus program

3. Switch your microscope' object lens to 4X (or 10X, 40X,100X ), focus the micro
ruler to get the clear image, move the ruler to the video center. Click the drop-down arrow

of the comb box to get the drop down

menu , select: Define Software

Power-> Horizontal Line. The user can also select Vertical Line or Any Line, the
precondition is that you must know the dimension between the clicked start point and end
point. When your video window is not selected in the maximum resolution mode, the
application will suggest you to set the Video Stream Format to the maximum resolution as
shown in Fig.3 . Select Setup->Video Stream Format... to set the video window resolution
to the maximum one. If the video resolution is in the device's maximum resolution, then
there should display a big cross and a small cross according to the setup of

36
the Measurement Setting menu (the drop-down menu of ).
The cursor position will also be shown near the small cross position.

Fig.3 The maximum Video Stream Format resolution prompt dialog

4. Put the cursor at the star point (any vertical line of the micro ruler ),click the left
mouse button ,the point will be marked with a small cross ,its coordinate will also be
displayed near the marked point. Move the mouse to the end point you want to mark, click
the left mouse button again and the end point will be marked, the final screen looks like
Fig. 4.

Fig.5 Use the horizontal line to

Fig.4 The marked start and end point for 4X
define the Software Power of the
objective power
4X objective lens

5. When the left mouse button is released in step 4, a Define Software Power dialog
box will be shown like Fig.5, here, Index 1 shows the start point coordinates and Index 2
shows the end point coordinates. the length between these two points will be displayed in
the length disabled edit box, the user should fill the Actual Length in edit box with the
right unit (here, it is 1000 micrometer). When the Actual Length is filled in, the OK
button will be enabled. If everything is OK, press or click OK button.

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 6. A dialog box called Software Power will be pop up, click Add...button, there should
pop up Add Software Power dialog box shown in Fig.7, Fill 4 (10,40,100) into the
Software Power edit box (means the current practical objective lens is 4x, 10X, 40X,
100X) .Click OK button.

Fig. 6 Software Power list view Fig.7 Add Software Power dialog box

7. The program will return to Software Power dialog box just the same as in Fig.
6 but 4X and its resolution equal to 35900 item is added in the list view as shown
in Fig.8.

Fig. 8 Software Power list view with 4X Fig.9 Software Power list view with
item added 4X,10X,40X,100X items added

8. Repeat step 3, 4, 5, 6, 7,In step 6, change the objective power in Fig.7 to the
practical power you switch to such as 10X,40X,100X.When the above operations are
finished ,The Software Power dialog box will be shown like Fig 9. Here user can Add,
Delete ( After selected ,click the Delete button in Fig.9 ) and change the order of the list of
the powers to what ever you want( Select the one you wish to change ,click Up or Down
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button ). Up to now, User can select the objective power from comb box
drop-down button on DirectShow Capture Window

Toolbar (The program will

add all of the Software Powers listed in Fig.9 to comb box after OK

button is clicked, and click drop-down button, the new comb box list

should look like this for user to select. At the same time, In File->Import

Image->Software Power submenu, the objective powers the microscope have will also
be added as shown in Fig.10. The user can select his current Software Power either

from this menu or from the comb box on the .DirectShow Capture

Window Toolbar .

Fig.10. The Software Power submenu after the Software Powers setup is finished

9. Video measurement: if your current microscope's objective power is 40X, then

select your Software Power from the comb box to 40X

and click drop-down button, select Length from the drop-down

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menu , the should be a big and a small alignment mark shown
in Fig, 11 (User can set their characteristic in Measurement setting... on

).Click the start point, move the mouse and click at the second
point (end point), after the mouse button is released, a dialog box list the object properties
will automatically pop up as shown in Fig,12

Fig.11 The measurement results under 40X Fig.12 The video measurement
objective power result

10. The user can measure the object angle, perform batch measurement in the video
window. The batch measurement is shown in Fig.13,it includes Length Measurement,
Angle Measurement and View Result...

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 Fig.13. The measurement submenu on the DirectShow Capture Window Toolbar

11. Except this, according to the practical object power, the user can select Software

Power power in comb box or In File->Import Image->Software

Power submenu shown in Fig10, click to capture image, define the layer in

the Tool Box Layer Page and perform the other complex draw operations.

12. In the previous sections we have discussed how to use the Horizontal Line,
Vertical Line and Any Line to define the Software Power, to increase the accuracy, we also
provide Define Software Power by

Average in drop-down

menu ,its principle is the same, it only need the user to draw the Horizontal Line, Vertical
Line and Any Line multiple times and allocates it to one objective power to get the average
resolution to delete or decrease the alignment error.

Please refer to the help content in the software help menu to find more information
about the power setup (calibration) operation.

5.4.3 Video Watermark

Fig.1 shows the video window image, it's actually a micro ruler. You can try to
extract the dark lines as the mark and overlay it with video in DirecShow Capture
Window:

1. Click to capture the image as shown in Fig.1.

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Fig.1 Image captured from the DirectShow
Fig.2 Image after binarized
Capture Window

2.Choose Process->Binary... to binarize the image, the result is shown in Fig.2.

3.Inverse the image using command Image->Adjust->Invert. Change the image to 24 bit
using Image->Color Quantize... The result is shown in Fig.3

Fig.3 Inverted binarized image Fig.4 Video Watermark setup dialog

4.Save the image in 24 bit BMP format.

5. Choose File->Image Import->Video Watermark..., there should pop up a Video

Watermark dialog as shown in Fig.4. Click button to find the image directory saved in
step 4. Set the Threshold in color, select Transparent (%) index to what ever you want,
Check or uncheck Use the marker color. Finally, select the Waterark Color. If everything
is Ok, click OK button, the final Video Watermark should look like Fig.5.

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 Fig.5 Video window with Video Watermark overlaid

6. User can press or down and using arrow keys to move or rotate the marker.
Please refer to DirectShow Capture Window Toolbar for detailed information.

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 Fig.6 Video Watermark moved and rotated

5.5 Image Processing

See the help contents in the software.

5.6 Image Browser

An Image Browser lets you efficiently find and organize your images.
To open the image browser, please select the menu ‘View->Browse’.

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The Browser Window Toolbar
ScopeImage Plus's Browser Window toolbar is shown as below:

Open an image file using file open dialog.

Save image file. Disabled in the browser window

Cut the selected image files
Copy the current selected image files
Paste image files from clipboard
Display /hide the browser folder.
Delete selected image file.
Execute this button to reload images.
Select thumbnail mode.
Arrange images by their name, type,size or by alphabet order.
Stop the icon loading of the image under current directory.

5.7 Help Contents

Click ‘Help->Help Contents’ or press ‘F1’ to find more information about how to use
the ScopeImage Plus software.

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Please refer to the index on the left side of the web page to get the information
you want.

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6. Use the Camera in ScopeImage Advanced
6.1 An overview of the ScopeImage Advanced
ScopeImage Advanced is a 24bits real color microscopy image
processing software based years of experience in the field of digital image
processing and analysis, which provided a large number of image
processing and measurement tools, suitable for all kinds of microscopy
image processing and measurement tasks. High reliability and robust
performance, easy to use, makes it a powerful tool to the user in scientific
and research fields.
Note:
Before using the ScopeImage Advanced, please insert the encryption lock
to your computer’s USB2.0 port, otherwise, the software will not be
activated.

6.2 Use Directshow to Preview in ScopeImage Advanced

(a) Open the Directshow Preview Window

1. Select video source. Click the menu ‘video ->Select video source’, there will come out a dialog for
you to choose video source, please select ‘Camera-H3C’, and remember to click the button “OK”.

2. Open the preview window. Click the menu ‘Video->Preview->Preview’. Or you can click the icon
on the toolbar.

or

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(b) Adjust the Color.
Please click the menu ‘Video->Preview->Video filter’ to open the video property window.

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(c) Do the Calibrating in ScopeImage Advanced.
Do the calibration first:
z Begin direct show preview
z Put the micro ruler in the video window
z Adjust the direction of the micro ruler to let it parallel to the horizontal mark
z Take a picture
z Open the Dimension calibration dialog, as follows:

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Here we use the 100um micro ruler, move to adjust the cross line, set the ‘X Size’ and ‘Y Size’,
be sure to make the value of X um/pixel and Y um/pixel is the same.
Now you can do the measurement.

You can see the result in the measurement data bar.

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6.3 Use Twain to Preview in ScopeImage Advanced
1. Select a scanner; open the file menu->Select scanner, there will come out a dialog for you to choose
the device, please choose ‘Camera-H3C’, as follows:

2. Second, open File menu-> Scan or you can click the icon on the toolbar.

or
3. Now comes out the video preview window, you can preview and operate.

Please refer to the ‘ScopeImage Advanced User Manual’ to get more

information about how to use the ScopeImage Advanced software.

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7. Trouble shooting

Never disassembly the instruments yourself!

If you have any problems with the instrument quality or the operation,
please contact with us or the distributor where you bought the instrument.
Please never disassembly the instrument yourself because this may cause
other problems or damage.

USB Device conflict

When your computer’s main board connects with more than two USB
devices, some unknowable errors will be happen; the errors contain the
deficient USB bandwidth and the insufficient USB power supply and so on.
Please plug out these USB device not use currently, and restart your
computer, it may repair these errors. Sometimes you need to uninstall
your device and reinstall the driver again.

Image Morphing
It is because the computer’s display resolution is mismatch with the screen
resolution. So please reset the screen resolution to avoid the image morphing. Please do
as follows:
（1） Right click the mouse on the desktop, and click ‘Properties’;
（2） A ‘Display Properties’ dialog will pop up, click the ‘Settings’ properties page:

（3） Check the Screen resolution, whether it is suitable with your monitor screen. If not,
please change it.

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Application fails to start
If there comes out the error dialog when you launch the application ‘ScopeImage
Plus’

Please Install the under the CD folder ‘ActiveX’.

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