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Negative Positive: Test. API 10 S

The document provides instructions for performing biochemical tests using an API 10 kit to identify bacteria. Key steps include inoculating colonies into water or saline, filling test tubes, reading results the next day except for three tests requiring additional reagents, and interpreting the color changes using a reference chart to determine the bacteria identification number.

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463 views

Negative Positive: Test. API 10 S

The document provides instructions for performing biochemical tests using an API 10 kit to identify bacteria. Key steps include inoculating colonies into water or saline, filling test tubes, reading results the next day except for three tests requiring additional reagents, and interpreting the color changes using a reference chart to determine the bacteria identification number.

Uploaded by

dr
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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API 10 TEST

Perform Oxidase test and note result.


Put 3 ml Distilled Water into tray.
Put single colony into 5 ml of D/water or Normal saline.
Fill the tubes.
Next Day read all results except TDA, Indole, NO2.
In TDA add one drop of TDA reagent.
In Indole add one drop of IND reagent.
In Glucose add one drop of NIT 1 and one drop of NIT 2.
Test. API 10 S Negative Positive
ONPG Colorless Yellow/ Pale Yellow
Glucose Blue/Blue Green Yellow/Yellow-grey
ARA Blue/Blue Green Yellow
LDC Oil Yellow Red/Orange
ODC Oil Yellow Red/Orange
CITRATE Full Pale Green/Yellow Blue Green/Blue in
upper part
Black deposit / Thin
H2 S Oil Colorless/grayish
Line
Urea Oil Yellow Red/Orange
TDA Yellow Reddish Brown
immediate
Colorless /Pale Green /
INDOLE Yellow Pink Immediate

OXIDASE Colorless Purple Blue


In Glucose add 1 drop Nit 1 + 1 drop Nit 2 ….see after 2-5 minutes
NO2 Yellow Red

ONPG Glu ARA LDC ODC CIT H2S Urea TDA IND OX NO2

1 2 4 1 2 4 1 2 4 1 2 4

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