Material Safety Data Sheet: BCA Protein Assay Reagent A, 500 ML

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Material Safety Data Sheet


Conforms to 93/112/EC and ISO 11014-1
Responsible Name Pierce Administration
Section 1. Chemical Product and Company Identification
Product name BCA Protein Assay Reagent A, 500 ml
Product No. 0023228
Supplier In USA:In Europe: Manufacturer Pierce Biotechnology
Pierce Perbio Science P.O. Box 117
P.O. Box 117
Industriezone III Rockford, IL 61105
Rockford, IL 61105
Industrielaan 27 USA
USA 9320 Erembodegem-Aalst 815.968.0747 or
815.968.0747 or
Belgium 1.800.874.3723
1.800.874.3723
Tel:+32 53 83 44 04
Fax:+32 53 83 76 38
In Case of Emergency CALL CHEMTREC: Print Date 11/12/2002
800.424.9300 Validation Date
OUTSIDE US:
11/4/2002
202.483.7616 MSDS# 3971

Synonyms Bicinchoninic Acid Protein Assay Reagent A

Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.

Section 2. Composition, Information on Ingredients


Substance/Preparation : Preparation

Ingredient Name CAS No. % EC Number Symbol R-Phrases


1) BCA Reagent A Ingredient 3 1-3 Not available. -- Not controlled under
dsd (Europe).
2) Sodium Carbonate, Anhydrous 497-19-8 1-3 207-838-8 Xi R36/37/38

Section 3. Hazards Identification


United States Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s)
for the proper handling of institutional materials/equipment associated with the use of this product.
Emergency Overview WARNING!
CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS:
MUCOUS MEMBRANES, RESPIRATORY TRACT, SKIN, EYES.

Routes of Entry Absorbed through skin. Eye contact. Ingestion.


Potential Acute Health Effects Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of inhalation.
Carcinogenic Effects Data CARCINOGENIC EFFECTS: Classified None. by NIOSH [Sodium Bicarbonate]. Classified 4 (No evidence.) by NTP,
None. by NIOSH [Sodium Tartrate Dihydrate]. Classified None. by NIOSH [Sodium Carbonate, Anhydrous].
MUTAGENIC EFFECTS: Not available.
TERATOGENIC EFFECTS: Not available.
Medical Conditions Aggravated Repeated or prolonged exposure is not known to aggravate medical condition.
by Overexposure:
Overexposure /Signs/Symptoms Not available.

Europe

Classification Not controlled under dsd (Europe).


Physical/chemical hazards Not applicable.
Human health hazards Not applicable.
Environmental hazards Not applicable.

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BCA Protein Assay Reagent A, 500 ml Page: 2/6

See Toxicological Information (section 11)

Section 4. First Aid Measures

Notice to Reader Get immediate medical attention.


Effects and symptoms
Inhalation Slightly hazardous in case of inhalation.
Ingestion Slightly hazardous in case of ingestion.
Skin Contact Sensitization of the product: Not available.
Slightly hazardous in case of skin contact (irritant). Skin inflammation is characterized by itching, scaling, reddening, or,
occasionally, blistering.
Eye Contact Slightly hazardous in case of eye contact (irritant).
Aggravating conditions Repeated or prolonged exposure is not known to aggravate medical condition.

First-Aid Measures
Inhalation If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical
attention if symptoms appear.
Ingestion Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious
person. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms appear.
Skin Contact Wash with soap and water. Cover the irritated skin with an emollient. Get medical attention if irritation develops. Cold water
may be used.
Eye Contact Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Cold water may be used. Get medical attention if irritation occurs.
Notes to Physician Not available.
Protection of first-aiders Not available.

Section 5. Fire Fighting Measures

Flammability of the Product Non-flammable.


Flash Points Not applicable.

Fire Hazards in Presence of Various Not applicable.


Substances

Fire Fighting Media Not applicable.


and Instructions
Protective Clothing (Fire) Not applicable.

Hazardous thermal (de)composition Not applicable.


products

Section 6. Accidental Release Measures

Personal precautions Safety glasses. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves.

Environmental Precautions and Absorb with an inert material and put the spilled material in an appropriate waste disposal. Finish cleaning by spreading
Clean-up Methods water on the contaminated surface and allow to evacuate through the sanitary system.
Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container.
Finish cleaning by spreading water on the contaminated surface and dispose of according to local and regional authority
requirements.

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BCA Protein Assay Reagent A, 500 ml Page: 3/6

Section 7. Handling and Storage

Handling Avoid breathing vapors or spray mists.

Storage Keep container tightly closed. Keep container in a cool, well-ventilated area.
Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.
Packaging materials
Suitable / Not suitable Use original container.

Section 8. Exposure Controls/Personal Protection

Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their
respective threshold limit value.

Exposure Limit Values


Ingredient Name Occupational Exposure Limits
United States
1) Sodium Carbonate, Anhydrous Not available.

Sweden
1) BCA Reagent A Ingredient 3 Not available.
2) Sodium Carbonate, Anhydrous Not available.

Denmark
1) BCA Reagent A Ingredient 3 Not available.
2) Sodium Carbonate, Anhydrous Not available.

Norway
1) BCA Reagent A Ingredient 3 Not available.
2) Sodium Carbonate, Anhydrous Not available.

France
1) BCA Reagent A Ingredient 3 Not available.
2) Sodium Carbonate, Anhydrous Not available.

Netherlands
1) BCA Reagent A Ingredient 3 Not available.
2) Sodium Carbonate, Anhydrous Not available.

Germany
1) BCA Reagent A Ingredient 3 Not available.
2) Sodium Carbonate, Anhydrous Not available.

Personal Protection
Eyes Splash goggles.
Body Lab coat.
Hands Gloves.
Respiratory Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits.
Protective Clothing
(Pictograms)

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BCA Protein Assay Reagent A, 500 ml Page: 4/6

Section 9. Physical and Chemical Properties

Physical State and Liquid. Odor Not available.


Appearance

Molecular Weight Not applicable. Taste Not available.

pH (1% Soln/Water) Neutral. Color Not available.

Boiling/Condensation Point The lowest known value is 100°C (212°F) (Milli-Q Water).
Melting/Freezing Point May start to solidify at -0.1°C (31.8°F) based on data for: Milli-Q Water.
Specific Gravity Weighted average: 1.01 (Water = 1)
Vapor Pressure The highest known value is 2.3 kPa (@ 20°C) (Milli-Q Water).
Vapor Density The highest known value is 0.62 (Air = 1) (Milli-Q Water).
Volatility 0% (w/w). ( Sodium Tartrate Dihydrate.)
Evaporation Rate 0.36 (Milli-Q Water) compared to Butyl acetate.
VOC -98 (%)
Dispersion Properties See solubility in water.

Solubility Easily soluble in cold water.


Soluble in hot water.

Section 10. Stability and Reactivity


Stability and Reactivity The product is stable.
Conditions to avoid Not available.
Materials to avoid Slightly reactive to reactive with metals, acids.

Hazardous Polymerization Will not occur.

Hazardous Decomposition Not applicable.


Products

Section 11. Toxicological Information

Toxicity to Animals Acute oral toxicity (LD50): 4090 mg/kg [Rat]. (Sodium Carbonate, Anhydrous).

Chronic Effects on Humans CARCINOGENIC EFFECTS: Classified None. by NIOSH [Sodium Bicarbonate]. Classified 4 (No evidence.) by NTP,
None. by NIOSH [Sodium Tartrate Dihydrate]. Classified None. by NIOSH [Sodium Carbonate, Anhydrous].
Other Toxic Effects on Humans Slightly hazardous in case of eye contact (irritant), of ingestion, of inhalation.

Special Remarks on Toxicity to Not available.


Animals
Special Remarks on Chronic To the best of our knowledge, the toxicological properties of this substance have not been thoroughly investigated.
Effects on Humans
Special Remarks on Other Toxic To the best of our knowledge, the toxicological properties of this product have not been thoroughly investigated.
Effects on Humans

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BCA Protein Assay Reagent A, 500 ml Page: 5/6

Section 12. Ecological Information

Mobility Not available.


Persistence/degradability Not available.
Bioaccumulative potential Not available.
Ecotoxicity Not available.
Germany water class VCI WGK: No products were found.

Section 13. Disposal Considerations


Waste Information Waste must be disposed of in accordance with federal, state and local environmental control regulations.
Waste Stream Not available.

Consult your local or regional authorities.

Section 14. Transport Information


Contact the supplier for all information regarding the proper transportation method for this material.

Section 15. Regulatory Information

Label Requirements
(Europe) This product is not classified according to the EU regulations.

HCS Classification Target organ effects.

U.S. Federal Regulations TSCA 8(b) inventory: Sodium Bicarbonate; BCA Reagent A Ingredient 3; Sodium Carbonate, Anhydrous; Milli-Q Water
TSCA 8(d) H and S data reporting: Sodium Bicarbonate
SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: Sodium Carbonate, Anhydrous
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Sodium Carbonate, Anhydrous: immediate health hazard, delayed
health hazard
SARA 313 toxic chemical notification and release reporting: No products were found.
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: No products were found.
Clean air act (CAA) 112 accidental release prevention: No products were found.
Clean air act (CAA) 112 regulated flammable substances: No products were found.
Clean air act (CAA) 112 regulated toxic substances: No products were found.

WHMIS (Canada) Not controlled under WHMIS (Canada).

CEPA DSL: Sodium Bicarbonate; BCA Reagent A Ingredient 3; Sodium Carbonate, Anhydrous; Milli-Q Water

International Regulations

EINECS Not available.

DSCL (EEC) This product is not classified according to the EU regulations.


International Lists

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BCA Protein Assay Reagent A, 500 ml Page: 6/6
Australia (NICNAS): Sodium Bicarbonate; Sodium Tartrate Dihydrate; Sodium Carbonate, Anhydrous; Milli-Q Water

Germany water class: Sodium Bicarbonate; Sodium Carbonate, Anhydrous

Korea (TCCL): Sodium Bicarbonate; Sodium Carbonate, Anhydrous; Milli-Q Water

Philippines (RA6969): Sodium Bicarbonate; Sodium Carbonate, Anhydrous; Milli-Q Water


State Regulations No products were found.
California prop. 65: No products were found.

Section 16. Other Information

Hazardous Material Health * 1 National Fire Protection


Fire Hazard
Information System
(U.S.A.) Fire Hazard 0
Association (U.S.A.) 0
Reactivity 0
Health 1 0 Reactivity

Specific Hazard
Personal Protection g

References Not available.


History of Document Changes Any information changes since last document version are marked with a triangle symbol.
Full text of R-Phrases Not applicable.
referenced under headings 2 and 3: Irritating to eyes, respiratory system and skin.

Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications.

Validated by Pierce Administration on 11/4/2002. Verified by Pierce Administration. Date of Previous Issue No Previous
Validation
Printed 11/12/2002. Version 0.01
Notice to Reader
To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes any
liability whatsoever for the accuracy or completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.
Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

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