Activity 3 Structure of A Plant Cell
Activity 3 Structure of A Plant Cell
Objectives
*To identify the basic parts of a plant cell
*To compare the shape, structure and function of some
plant cells
Specimens
Onion (Allium cepa), digman (Hydrilla or Elodea), tomato
(Solanum lycopersicum), squash stem (Cucurbita maxima)
http://teachline.ls.huji.ac.il/72346/PlantCell
Materials
Compound microscope, cover glass, glass slides, water, iodine solution
PROCEDURE
1. Onion
a. Take an onion bulb and peel off a small piece of epidermis with forceps and scissors.
b. Put the epidermis specimen on the glass slide and flood with a drop of iodine solution.
c. Put a coverslip and examine under LPO and HPO.
2. Tomato
a. Prepare a temporary mount of thin epidermal covering of a ripe tomato fruit.
b. Put a small piece of the epidermis on the glass slide with a drop of iodine solution,
then cover with a cover slip
c. Examine under LPO and HPO.
3. Digman
a. Pull out a single leaf from a young shoot of hydrilla plant that has been exposed to
sunlight.
b. Examine under LPO and HPO. You might notice the streaming movement of the
cytoplasm due to the movement of chloroplasts.
4. Squash stem
a. Mount at least three (3) trichomes from a squash stem.
b. Put a drop of iodine solution into the glass slide
c. Examine closely under LPO and HPO.
RESULTS
A. Onion
Draw 4 cells under LPO and 1 cell under HPO. Label the visible parts.
________________________________ ________________________________
B. Tomato
Draw 4 cells under LPO and 1 cell under HPO. Label the visible parts.
________________________________ ________________________________
C. Digman
Draw 4 cells under LPO and 1 cell under HPO. Label the visible parts.
D. Squash Trichomes
________________________________ ________________________________
D. Squash Trichomes
Draw 4 cells under LPO and 1 cell under HPO. Label the visible parts.
E. Squash Trichomes
________________________________ ________________________________
QUESTIONS
1. How do cells of the onion specimen appear under the microscope?
____________________________________________________________________________
____________________________________________________________________________
2. Are the cell walls thick and are the nuclei invisible in the onion epidermal cells?
____________________________________________________________________________
____________________________________________________________________________
3. What shape do the epidermal cells of tomatoes exhibit? What is the color of the dominant
pigments here?
____________________________________________________________________________
____________________________________________________________________________
4. Did you notice the movement of chloroplastids? Describe what you have observed.
____________________________________________________________________________
____________________________________________________________________________
5. What is the shape of the apical and marginal cells of Hydrilla?
____________________________________________________________________________
____________________________________________________________________________
6. In plant trichomes, did you see a prominent nucleus? Is the trichome septate?
____________________________________________________________________________
____________________________________________________________________________
7. What are trichomes for?
____________________________________________________________________________
____________________________________________________________________________
*Adapted from Laboratory Worktext in General Botany by Evangelista E. and Luisito Evangelista. 2009