This document contains 12 multiple choice questions about analytical chemistry techniques and concepts. The questions cover topics like determining organic acid basicity, detecting amino acids in thin layer chromatography, carrier gases for gas chromatography, separation mechanisms for gel chromatography and ion-exchange chromatography, distinguishing D and L isomers, sodium vapor lamp wavelengths, stationary phases for porphyrin separation, sephadex composition, sample size requirements for immersion refractometry, and classifications of solvents like glacial acetic acid.
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Pharm Analysis-II Model Answer
This document contains 12 multiple choice questions about analytical chemistry techniques and concepts. The questions cover topics like determining organic acid basicity, detecting amino acids in thin layer chromatography, carrier gases for gas chromatography, separation mechanisms for gel chromatography and ion-exchange chromatography, distinguishing D and L isomers, sodium vapor lamp wavelengths, stationary phases for porphyrin separation, sephadex composition, sample size requirements for immersion refractometry, and classifications of solvents like glacial acetic acid.
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1.
1 Basicity of organic acids can be determined by
(a) Conductometry (b) Refractometry (c) Non-aqueous titrations (d) Complexometry 1.2 For the detection of amino acids in Thin Layer Chromatography, the best regent is (a) ninhydrin (b) copper sulphate (c) iodine (d) potassium permanganate 1.3 Which of the following can not be used as carrier gas in gas chromatography (a) Hydrogen (b) Nitrogen (c) Helium (d) oxygen 1.4 Gel chromatography method separates different substances depending on their (a) molecular size (b) molecular weight (c) density (d) viscosity 1.5 To explain the column efficiency, two theories i.e., plate and rate theory has been proposed. They are related to (a) HPLC (b) Gel chromatography (c) Gas liquid chromatography (d) Paper chromatography 1.6 The D and L isomeric forms can be distinguished by (a) Polarimetry (b) Refractometry (c) Potentiometry (d) Conductometry 1.7 Sodium vapor lamp used in Polarimeter emit light of wavelength (in Angstrom) (a) 5890 & 5896 (b) 4368 & 4916 (c) 5770 & 5791 (d) 5461 & 4368 1.8 Porphyrins can be separated using which stationary phase (a) magnesium carbonate (b) alumina (c) aluminium silicate (d) silica gel 1.9 The composition of sephadex used as stationary phase in gel chromatography is (a) starch (b) polyacrylamide (c) cross linked dextran (d) polyvinyl acetate 1.10 The principle of separation in ion-exchange chromatography is (a) adsorption (b) Partition (c) reversible exchange of functional groups (d) chemical reaction 1.11 Immersion refractometer requires how much sample for determination of refractive index (a) 2-5 ml (b) 10-15 ml (c) 40-50 ml (d) more than 100 ml 1.12 Glacial acetic acid is an example of (a) protogenic solvent (b) protophillic solvent (c) amphiprotic solvent (d) Aprotic solvent