Samples Reagent Observation Inference Egg White Hydrochloric Acid

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3.

Concentrated Mineral Acids


Samples Reagent Observation INFERENCE
Egg White Hydrochloric
acid

Egg
Albumin

Casein

Gelatin

Table 3.1. Results of the Concentrated Mineral Acid; HCl to Egg white, Egg albumin, Casein,
and Gelatin.
Samples Reagent Observation INFERENCE
Egg White Sulfuric
acid

Egg
Albumin

Casein

Gelatin

Table 3.2. Results of the Concentrated Mineral Acid; Sulfuric acid to Egg white, Egg albumin,
Casein, and Gelatin.
Samples Reagent Observation INFERENCE
Egg White Nitric acid

Egg
Albumin

Casein

Gelatin

Table 3.3. Results of the Concentrated Mineral Acid; Nitric acid to Egg white, Egg albumin,
Casein, and Gelatin.
Principle: Concentrated acid like concentrated HCl, H2SO4, HNO3 and alkali alter the ionization
of the carboxyl and amino groups, disrupting the salt linkages and hydrogen bonds. Altered
ionization leads to a change in the isoelectric point of the protein.
Precipitation occurs after addition but redissolves upon shaking. Hydrolysis may occur which
could further degrade the protein.

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