Samples Reagent Observation Inference Egg White Hydrochloric Acid
Samples Reagent Observation Inference Egg White Hydrochloric Acid
Samples Reagent Observation Inference Egg White Hydrochloric Acid
Egg
Albumin
Casein
Gelatin
Table 3.1. Results of the Concentrated Mineral Acid; HCl to Egg white, Egg albumin, Casein,
and Gelatin.
Samples Reagent Observation INFERENCE
Egg White Sulfuric
acid
Egg
Albumin
Casein
Gelatin
Table 3.2. Results of the Concentrated Mineral Acid; Sulfuric acid to Egg white, Egg albumin,
Casein, and Gelatin.
Samples Reagent Observation INFERENCE
Egg White Nitric acid
Egg
Albumin
Casein
Gelatin
Table 3.3. Results of the Concentrated Mineral Acid; Nitric acid to Egg white, Egg albumin,
Casein, and Gelatin.
Principle: Concentrated acid like concentrated HCl, H2SO4, HNO3 and alkali alter the ionization
of the carboxyl and amino groups, disrupting the salt linkages and hydrogen bonds. Altered
ionization leads to a change in the isoelectric point of the protein.
Precipitation occurs after addition but redissolves upon shaking. Hydrolysis may occur which
could further degrade the protein.