Good Morning/Good Afternoon...

, I am ________________,

I am ________________

And I am……….

The researchers would like to propose a research investigation entitled Phytochemical Screening, Nutrient
Analysis and Antimicrobial Assay of Rambutan (Nephelium iappaceum) Exocarp, Leaves, Fruit and Seed.

(Brettnia): Will explain why we chose rambutan as the subject of study to be conducted.

(Jhon Dave): Will introduce the Gap and Contradictions (Which is the difference and similarities of our study
from the others). Explain the first Author Shihabudeen, M., Priscilla, H., and Thirumurugan, D. (2010).
And also sa study nila Umaru, I., Badruddin, F. and Umaru H. (2019)

Text (Kindly summarize ang iingon sa board members):

The researchers, Shihabudeen, M., Priscilla, H., and Thirumurugan, D. (2010) carried out an
investigation entitled “Antimicrobial activity and phytochemical analysis of selected Indian folk medicinal
plants.” The study undertaken has addressed several aspects similar to the current study particularly the
methods that were used which are the Disc diffusion method, Minimum Inhibitory Concentration (MIC) method,
Spirit Collection of Herbs, Floral Dissection and Description, and Swabbing of Inoculum on Mueller Hinton
Plate. In addition, both studies used the same statistical tool, One-way ANOVA. Conversely, the researchers
have also identified a demographical gap in between the prior research and the current study. It has been
shown that their study was conducted in South India, however the current study will be conducted and limited
only in the City of Malaybalay, Bukidnon.

The study conducted by Umaru, I., Badruddin, F. and Umaru H. (2019) with the title, “Phytochemical
Screening of Essential Oils and Antibacterial Activity and Antioxidant Properties of Barringtonia asiatica Leaf
Extract,” has presented numerous features similar to the current study, mainly, the preparation of Test
Sample, which they used the Disc Diffusion method. Moreover, both studies are analogous since they observed
the potential of the crude extract of the Variable to be used against Gram-negative as well as Gram-
positive bacteria and also in the Plate Inoculation. Yet, the recent study have also shown various differences
in the methods utilized, comprising DPPH (2, 2-Diphenyl-1-picrylhydrazyl) Free-Radical Scavenging Assay
from the Crude Extract, Preparation of Bacterial Broth, and the Statistical Analysis considering that the recent
study used Statistical analysis for antibacterial activities was performed using SPSS programme.

(Jansen): You will be the next person to state the Gap and Contradictions sa study nila Ebana, B., Asamudo,
N., Etok, C., Edet, U., and Onyebuisi, C. (2016). And also ila Bhardwaj, A., Sharma1, D., Jadon, N.
and Agrawal1, P. (2015)

Text (Kindly summarize ang iingon sa board members):

“Phytochemical Screening, Nutrient Analysis and Antimicrobial Activity of the Leaves of Lasianthera
africana and Dennettia tripetala on Clinical Isolates,” carried out by Ebana, B., Asamudo, N., Etok, C., Edet,
U., and Onyebuisi, C. (2016), has shown factors parallel to the current study in terms of what it aims to
identify, specifically the Nutrient Analysis and Phytochemical Characteristics of their selected variable.
However, the researchers identified an apparent theoretical gap in the prior research concerning its
theoretical basis. The previous theory tends to focus primarily on the information gathered in their locality.

Multiple studies had been done in relation to the current research study, including the research study
investigated by Bhardwaj, A., Sharma1, D., Jadon, N. and Agrawal1, P. (2015) entitled “Antimicrobial and
Phytochemical Screening of Endophytic Fungi Isolated from Spikes of Pinus roxburghii.” This study has sighted
common features to the current study of the researchers. Both Studies brought the collected samples to the
laboratory in sterile bags and processed within a few hours after sampling to reduce the chance of
contamination. Furthermore, both studies also aims to identify the bioactive components present in the
variable and its potential to fight against pathogenic microorganisms.
(Brettnia): Will be the last one to state the Gap and Contradictions sa study nila Kelmanson, J., Jager, A., and
Staden, J. (2000).

Text (Kindly summarize ang iingon sa board members):

“Zulu medicinal plants with antibacterial activity,” mainly conducted by Kelmanson, J., Jager, A., and
Staden, J. (2000), showed multiple similarities to the current study. Mutually, both studies have the same
method of the screening of the antimicrobial activity of the variable specifically the disc-diffusion assay. They
are also similar in getting the MIC (Minimal inhibitory concentrations) through the method, Agar Diffusion
Method. Nevertheless, the studies have also differences and has shown a gap between their subjects or the
plant material to be studied; the extracting of plant material and how the results would be gathered and
tabulated.

(Brettnia): Will be the One to state the researcher’s theological basis of the study to be conducted.

Text (Kindly summarize ang iingon sa board members):

According to the World Health Organization (2018), antimicrobial resistance (AMR) and antibiotic
resistance are serious threats to global health, security, development, and prevention and treatment of an
ever-increasing range of infections caused by bacteria, viruses, fungi, and parasites. AMR is a serious threat
to global public health, making it one of the world’s most pressing public health problems, and needs to be
averted with the help of all government sectors and society. Another public health problem is the naturally
occurring antibiotic resistance, which accelerates the process when antibiotics are misused in both humans and
animals. Both of these are lethal since they are becoming harder to treat as the antimicrobials and antibiotics
used on them for treatment are becoming less effective. This gave the researchers the idea to make
antimicrobials and antibiotics from alternative sources to help prevent infections from spreading and how
these source materials are capable of being used as medicine to cure diseases.
A collaboration between the Department of Health (DOH), World Health Organization (WHO),
Department of Agriculture (DA), World Organization for Animal Health (OIE), and Food and Agriculture
Organization (FAO) helped raise awareness on the threat of antimicrobial resistance (AMR) and encourage
all government sectors to address this problem. The effort to address the threat of AMR worldwide is
fortified. With the statement given by the Department of Health, the researchers were given the idea to help
control the spreading of diseases and regulate the usage of antibiotics.

(Jhon Dave): Will be the one to explain the Rationale:

Text (Kindly summarize ang iingon sa board members):

As stated by NBCI (National Center for Biotechnology Information) 2019, enough studies and
advancement in the field of medicine should be carried out because the resistant bacteria emergence is
occurring worldwide. The antibiotics which have transformed medicine is now critically endangered and the
spate of resistance to conventional antibiotics is becoming worrisome.
Infectious diseases caused by resistant microorganisms are associated with prolonged hospitalization,
increase cost and greater side for morbidity and mortality. On the current data by AMR (Action Plan on
Antimicrobial Resistance), 22 countries primarily investigated for pathogens isolated from blood, urine, stool
cervical and urethral specimens and this showed tremendous range in the proportion of bacteria that we are
not susceptible to one or more of the antibiotics commonly used to treat them.

(Jansen): Will be the one to explain the aim of the researchers:

In Solution to this emerging problem, plants are investigated for bioactive components with
antimicrobial activity. The researchers want to conduct this study to seek for a possible alternative source of
antibiotics.
ADDITIONAL INFO PARA SA MGA QUESTIONS SA BOARD SA ATO:

Kindly study the flow kung unsa atong buhaton

THAT WOULD BE ALL! THANK YOUU MGA PHILOSOPHERSSS!