Scribd Logo
Upload

Welcome to Scribd!

  • 49 views

    Alcian Blue Staining Kit (Ablue) : Product Description

    Uploaded by

    Bergita Paulino
    This document provides product information and instructions for the Alcian Blue Staining Kit, which is used to detect cell chondrogenesis by staining sulfated proteoglycans in cartilage tissue blue. The kit contains ready-to-use Alcian Blue and Nuclear Fast Red solutions, as well as other reagents. Instructions are provided for staining formaldehyde-fixed tissue sections using the kit. An example shows Alcian Blue staining of cartilage in cells cultured in chondrogenic medium, but not in cells cultured in regular growth medium.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Alcian Blue Staining Kit (Ablue) : Product Description

    Uploaded by

    Bergita Paulino
    49 views2 pages
    This document provides product information and instructions for the Alcian Blue Staining Kit, which is used to detect cell chondrogenesis by staining sulfated proteoglycans in cartilage tissue blue. The kit contains ready-to-use Alcian Blue and Nuclear Fast Red solutions, as well as other reagents. Instructions are provided for staining formaldehyde-fixed tissue sections using the kit. An example shows Alcian Blue staining of cartilage in cells cultured in chondrogenic medium, but not in cells cultured in regular growth medium.

    Original Description:

    alcian blue

    Original Title

    Ab Staining

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides product information and instructions for the Alcian Blue Staining Kit, which is used to detect cell chondrogenesis by staining sulfated proteoglycans in cartilage tissue blue. The kit contains ready-to-use Alcian Blue and Nuclear Fast Red solutions, as well as other reagents. Instructions are provided for staining formaldehyde-fixed tissue sections using the kit. An example shows Alcian Blue staining of cartilage in cells cultured in chondrogenic medium, but not in cells cultured in regular growth medium.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    49 views2 pages

    Alcian Blue Staining Kit (Ablue) : Product Description

    Uploaded by

    Bergita Paulino
    This document provides product information and instructions for the Alcian Blue Staining Kit, which is used to detect cell chondrogenesis by staining sulfated proteoglycans in cartilage tissue blue. The kit contains ready-to-use Alcian Blue and Nuclear Fast Red solutions, as well as other reagents. Instructions are provided for staining formaldehyde-fixed tissue sections using the kit. An example shows Alcian Blue staining of cartilage in cells cultured in chondrogenic medium, but not in cells cultured in regular growth medium.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Alcian Blue Staining Kit

    (ABlue)
    Catalog #8378

    Product Description
    Alcian Blue is a dye used to detect cell chondrogenesis and i t stains the sulfated proteoglycan in cartilage
    tissue. The Alcian Blue Staining Kit contains 1% Alcian Blue Solution, 0.1% Nuclear Fast Red in convenient,
    ready-to-use solutions. At pH 2.5, Alcian Blue stains acid mucopolysaccharides and shows a blue color, while
    Nuclear Fast Red stains nuclei pink to red and the cytoplasm pale pink [1].

    Kit Components

    Cat. No. # of vials Name Quantity Storage


    8378a 1 1% Alcian Blue Solution 100 mL Room temperature
    8378b 1 0.1% Nuclear Fast Red 100 mL Room temperature
    8378c 1 3% Acetic Acid 100 mL Room temperature
    8378d 1 Xylene Substitute 100 mL Room temperature

    Materials Supplied by User


    Formaldehyde-fixed and paraffin-embedded tissue sections
    Ethanol (100%, 95%, 70%, 50%)
    Deionized H2O (diH2O)

    Product use
    ABlue is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro
    diagnostic procedures.

    Shipping
    Room temperature.

    References
    [1] Steedman, H.F. (1950) Alcian blue 8GS: A new stain for mucin. Quarterly Journal of Microscopic Science,
    Vol 91, p477-479.

    Procedures
    1. Deparaffinize and hydrate slides:
    1) Deparaffinize the tissue sections in Xylene Substitute (Cat. #8378d), 3 changes of 5 minutes per change.
    2) Hydrate in 100% ethanol, 2 changes of 2 minutes per change.
    3) Hydrate in 95% ethanol, 2 changes of 2 minutes per change.
    4) Hydrate in 70% ethanol for 2 minutes.
    5) Hydrate in 50% ethanol for 2 minutes.

    1
    6) Rinse in diH2O for 5 minutes.

    2. Incubate in 3% Acetic Acid (Cat. #8378c) for 3 minutes.

    2. Stain in 1% Alcian Blue Solution, pH 2.5 (Cat. #8378a) for 30-60 minutes.

    3. Wash in running tap water for 2 minutes and rinse in diH2O.

    4. Stain in 0.1% Nuclear Fast Red (Cat. #8378b) for 10-20 minutes.

    5. Wash in running tap water for 1 minute and rinse in diH2O for 2 minutes.

    6. Dehydrate and clear slides:


    1) Dehydrate in 95% ethanol, 2 changes of 2 minutes per change.
    2) Dehydrate in 100% ethanol, 2 changes of 2 minutes per change.
    3) Clear the tissue sections in Xylene Substitute (Cat. #8378d), 2 changes of 2 minutes per change.

    7. Mount the tissue sections and observe under microscope.

    (a) (b)

    Figure 1. (a) HDF-f were cultured as pellets in complete MSC Chondrogenic Differentiation Medium
    (MCDM, Cat. # 7551) for 50 days. The pellets were fixed in 4% paraformaldehyde and sectioned.
    Alcian Blue staining demonstrated the presence of cartilage in cells (Magnification: 10X).

    (b) Human Dermal Fibroblasts-fetal (HDF-f, Cat. #2300) were cultured as pellets in growth medium,
    complete Fibroblast Medium (FM, Cat. #2301) for 50 days. The pellets were fixed in 4%
    paraformaldehyde and sectioned. Alcian Blue staining was not detected (Magnification: 10X).

    You might also like