Blood Wastage in A Tertiary Hospital Laboratory in Tacloban City
Blood Wastage in A Tertiary Hospital Laboratory in Tacloban City
Blood Wastage in A Tertiary Hospital Laboratory in Tacloban City
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A Research
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INTRODUCTION
Unnecessary tests, inefficient ordering practices, and collection of more blood than is
required for testing contribute to iatrogenic anemia in hospitalized patients. Blood is a valuable
resource and blood wastage in a low socio-economic country could impose a very serious impact
pediatric patient presenting with lethargy can get a series of blood tests including vitamin b12 ,
folate level & HbA1c to confirm megaloblastic anemia. Such tests are unnecessary to some
especially if there’s no appropriate questioning of the history of the patient. According to a survey,
of the 435 ER physicians asked about the tests they order for their patients, more than 85%
admitted that in general, they call for too many tests, even if they know the results won’t really
help them decide how to treat their patients (TIME, 2015) Imagine if hospitalized patients are
drawn with blood from time to time and knowing that these tests are unnecessary, this could
imposed something negative to the patients not only that it can cause iatrogenic anemia.
A relationship between the volume of blood drawn and iatrogenic anemia was first
described in 2005, when Thavendiranathan et al3 found that in adult patients on general medicine
floors, the volume of blood drawn strongly predicted decreased hemoglobin and hematocrit levels.
For every 100 mL of blood drawn, hemoglobin levels fell by an average of 0.7 g/dL, and 13.9%
of the patients in the study had iron studies and fecal occult blood tests performed to investigate
anemia. Kurniali et al reported that during an average admission, 65% of patients experienced a
drop in hemoglobin of 1.0 g/dL or more, and 49% developed anemia. Iatrogenic anemia from
blood draws is common, serious, and unnecessary. Salisbury et al, in 2011, studied 17,676 patients
with acute myocardial infarction across 57 centers and found a correlation between the volume of
blood taken and the development of anemia. On average, for every 50 mL of blood drawn, the risk
of moderate to severe iatrogenic anemia increased by 18%. They also found significant variation
in blood loss from testing in patients who developed moderate or severe anemia. The authors
believed this indicated that moderate to severe anemia was more frequent at centers with higher
than average diagnostic blood loss.5This relationship has also been described in patients in
intensive care, where it contributes to anemia of chronic disease. While anemia of critical illness
is multifactorial, phlebotomy contributes to anemia in both short- and long-term stays in the
In the laboratory setting, not all blood that is being drawn from the patients are
used. Only small amount of blood is needed for examinations especially that machines nowadays
have grown more sophisticated. These excess blood are not used anymore leading to wastage.
Research Objectives:
1. To determine the volume of blood used and the volume extracted in the desired
The findings which this study will reveal, may benefit certain groups and the benefits they
Future researchers. The results of this study will serve as an initial data for further studies on the
Clinical laboratories. With the results of this study, it may help hospitals and/or laboratories on
improving their policies, guidelines and protocols specifically on the area of blood extraction.
Medical Technologists. This study may help in giving knowledge or information to the medical
technologists about factors leading to wastage of blood samples and give them ideas on an
Patients. The results of the study may help minimize or lessen the incidences of unnecessary
excess blood extraction which will benefit patients in a way that they may be able to prevent
This study aims to confirm incidences regarding blood wastage in a hospital laboratory in
Tacloban City.
determine the factors that lead to sample wastage and the different ways excess samples are
disposed of. Printed questionnaires would be the basis for this study so as to have uniformity. The
researchers only obtain information from subjects who were able, willing, and qualified to
This research study is limited only within the school year 2018-2019.
Definition of Terms:
Clinical Laboratory -a laboratory where tests are usually done on specimens in order to obtain
information about the health of a patient as pertaining to the diagnosis, treatment and prevention
of disease.
Tertiary Hospital – a major hospital that usually has a full complement of services including
pediatrics, obstetrics, general medicine, gynecology, various branches of surgery and psychiatry.
Blood Wastage – refers to any blood component or product which is discarded rather than use
for testing
CHAPTER 2
This chapter presents readings on concepts and studies that serve as basis in the conduct of
the present study. Discussion focuses on resources that gave further insights into what have been
Related Literature
Blood is an essential component of every mortal. It has vital functions which are needed
for a person to subsist. This sticky fluid transports oxygen from the lungs to the cells of the body,
where it is needed for metabolism, and provides the cells with nutrients, transports hormones and
removes waste. Blood maintains homeostatic balance, it makes sure that the right body temperature
is maintained. It also protects blood vessel when damaged, certain parts of the blood clot together
very quickly and make sure that a scrape, for instance, stops bleeding. With all these significant
functions of the blood, it is then important to monitor and maintain its good condition by doing
often request laboratory test before concluding that a patient is suffering from a specific disease.
Effective use of basic laboratory tests at primary health care level significantly improves diagnosis
According to a study conducted by Carter et.al, Laboratory tests were ordered on 704
(62.1%) patients. Diagnosis and treatment were changed in 45% of tested patients who returned
with laboratory results (21% of all patients attending the clinics). 166 (23.5%) patients did not
return to the clinician for a final diagnosis and management decision after laboratory testing. Blood
slide examination for malaria parasites, wet preparations, urine microscopy and stool microscopy
resulted in most changes to diagnosis. There was no significant change in drug costs after
laboratory testing. The greatest changes in numbers of recorded diseases following laboratory
testing was for intestinal worms (53%) and malaria (21%). This study showed that laboratory test
The most commonly used sample in the laboratory is blood specifically serum. Commonly
requested blood test includes full blood count, renal profile, liver profile, lipid profile, blood
glucose, antibodies test, urines test, infectious disease screening, cancer test, heart test-cardiac
test,.( Door 2 Door Doctor, 2015). Full blood count contains complete blood count,one of the most
commonly ordered blood tests. It is the calculation of the cellular (formed elements) of blood.
These calculations are generally determined by special machines that analyze the different
components of blood in less than a minute. Vol of blood needed: 0.5 ml in EDTA, Vol of blood
extracted: 3 or 5 cc syringe is used. Renal profile give us a snapshot of your kidneys health. This
profile will include Blood Urea Nitrogen and Creatinine which is a by-product of protein
metabolism and is eliminated through the kidneys. This shows us how well your kidneys are
functioning and alerts us if there is any kidney failure. Among the electrolytes that are tested are
Sodium which is One of the major salts in the body fluid, sodium is important in the body’s water
balance and the electrical activity of nerves and muscles; Potassium helps to control the nerves
and muscles; and Chloride which helps to maintain the body’s electrolyte balance. Volume
extracted 90.5 ml, needed .3 ml. Serum is the preferred specimen. Liver Function Tests are one of
the more extensive screening profiles due to the vast number of functions that the liver performs.
These tests include: Protein is a measure of the state of nutrition in the body. Albumin in one of
the major proteins in the blood and a reflection of the general state of nutrition. Globulin is a major
group of proteins in the blood comprising the infection fighting antibodies. Bilirubin, chemical
involved with liver functions and is involved in digestion of fat. High concentrations may result in
jaundice which is a yellowish discolouration of skin and whites of the eyes. There are three major
liver enzymes which are Alkaline Phosphatase is a body protein important in diagnosing proper
bone and liver functions and can be raised if there is a obstruction of the billiary system; Aspartate
Aminotransferase (AST or SGOT)an enzyme found in skeletal and heart muscle, liver and other
organs. Abnormalities may represent liver disease; and Alanine Aminotransferase (ALT or SGPT)
an enzyme found primarily in the liver. Abnormalities may represent liver disease. Volume
extracted, 2 ml, minimum volume used 0.5 ml. Serum is the preferred specimen. Cholesterol Test
– Lipid Profile Test is a group of simple blood tests that reveal important information about the
types, amount and distribution of the various types of fats (lipids) in the bloodstream. Includes
Total Cholesterol, HDL (good) Cholesterol, LDL (bad) Cholesterol, Risk Ratio (good to total),
and Triglycerides. Too much cholesterol in the blood is a major cause of heart disease and blood
vessel disease. The high-density lipoprotein (HDL) cholesterol, which is referred to as the “good”
cholesterol because of its ability to take cholesterol and remove it from the arteries. The low-
density lipoprotein (LDL) cholesterol, or the “bad” cholesterol, which builds up and clogs the
arteries. Volume extracted, 1 ml, minimum volume used o.5 ml. Serum is the preferred specimen.
Blood Glucose Test , also known as A1c, HbA1c, Glycohemoglobin, or Glycated hemoglobin,
indicates how well you have controlled your diabetes over the last few months. Even though you
may have some very high or very low blood glucose values, Hemoglobin A1C will give you a
picture of the average amount of glucose in your blood over that time period. While the
Hemoglobin A1C is the standard tool to determine blood sugar control for patients with diabetes,
it is not a substitute for daily, routine blood glucose testing. Volume extracted, 1 ml, minimum
volume used 0.5 ml. Serum is the preferred specimen. Antibodies Test – Rheumatoid Factor is an
antibody that is measurable in the blood. Rheumatoid factor is actually an antibody that can bind
to other antibodies. Antibodies are normal proteins in our blood that are important parts of our
immune system. Rheumatoid factor is an antibody that is not usually present in the normal
individual. Most commonly, rheumatoid factor is used as a blood test for the diagnosis of
rheumatoid arthritis. Volume extracted, 4 ml, minimum volume used 1 ml. Whole blood is the
preferred specimen. Urine test Type of Blood Test - cancer test A routine urinalysis can tell your
doctor a great deal. Diabetes, urinary tract infections and kidney problems are among the many
medical conditions that can be detected by routine urinalysis results. Very often, a routine
urinalysis is part of a general physical exam. A routine urinalysis does not detect drugs of abuse.
A special drugs-of-abuse screening test is used to detect the presence of those substances. Volume
extracted, 4 ml, minimum volume used 1 ml. Serum is the preferred specimen. Infectious Disease
Screening Type of Blood Test - infectious diseases can be transmitted in a number of ways. If
you’ve been exposed, you want to know quickly, as diagnosis and treatment can help prevent
further transmission. Symptoms can indicate a disease, but lab tests for infection are the only way
to confirm a diagnosis Among Diseases that are commonly tested are HIV, Hepatitis, Influenza
and H-Pylori. Volume extracted, 1ml, minimum volume used 0.5 ml. Serum is the preferred
specimen. Cancer Test – Tumor markers detect the presence of certain types of cancer in the body,
and to monitor the progress of cancer treatment. Some examples of common tumor markers
include: CA15-3 is a sensitive tumor marker for breast cancer. CA19-9 antigen has been shown to
be elevated in the blood of some patients with gastrointestinal tumors. CA-125 is a useful marker
for monitoring disease progression in ovarian cancers. PSA is a useful marker for monitoring
disease progression in prostate cancers. CEA (carcinoembryonic antigen) is useful in monitoring
patients with various types of malignancies, such as gastrointestinal, pancreatic, breast, and lung
cancers. Volume extracted, 0.8ml, minimum volume used 0.3ml. Serum is the preferred specimen.
Heart Test – Cardiac Markers,elevated homo cysteine levels have a much greater risk of heart
attack or stroke than those with average levels. This can lead to heart attack, strokes, and blood
vessel blockages in any part of the body.Highly Selective C-Reactive Protein levels seem to be
correlated with levels of heart disease risk as well. Volume extracted, 1 ml, minimum volume used
0.5 ml. Serum is the preferred specimen. (Labcorp.com. Too much blood extraction can cause
an inpatient with normal Hgb on admission. While HAA is often multifactorial, phlebotomy losses,
especially in critically ill patients, is a major and potentially avoidable cause. As early as 1971,
Buckley‐Sharp showed that the hematocrit of hospitalized patients decreased proportionally to the
volume of blood collected for laboratory testing. Multiple studies report that HAA continues to be
a problem many decades later. For example, in a study including every adult admission at a large
academic center, 74% of patients developed HAA. When studying specific patient populations
such as those with renal impairment, in the intensive care unit (ICU), or hospitalized post‐trauma,
the incidence of HAA ranged from 20% to 70%. Salisbury et al. estimated that the risk of HAA
increased by 20% with each 50 mL of blood loss. In a recent editorial, Stefanini questioned the
need for the usual apparently excessive volume of blood requested for testing. It has also been
reported that laboratory testing and the corresponding blood volumes being collected are
outcomes. In one study, inappropriate laboratory testing was estimated to be as much as 50%.In
the Choosing Wisely campaign of the American Board of Internal Medicine (ABIM) Foundation,
several societies have included in their lists, the recommendation of avoiding laboratory tests at
regular intervals without a change in the patient's clinical status. At the same time, some authors
have challenged the notion that laboratory tests are a major cause of HAA. They propose that the
volume collected for tests is determined by the patient's severity of illness and ability to produce
RBCs to compensate for the lost blood. While a healthy person is expected to replace 1%, or about
50 mL of his/her total blood volume (TBV) per day, this capacity is impaired in hospitalized
individuals, lowering their threshold of tolerable blood loss. (Mann et.al, 2017)
CHAPTER 3
METHODOLOGY
This chapter presents the research design, description of the respondents, the research
instrument used, data gathering procedure, and the statistical treatment of the data gathered.
Research Design
This study utilized the descriptive – survey in order to achieve the purpose of this study-
which is to record sample wastage among clinical laboratories in Tacloban City area. It is
descriptive in a sense that the data were collected without changing the environment in which
nothing was manipulated. The information collected concerns about the occurrence of blood
wastage in clinical laboratories within hospitals and methods involved survey which describes the
Research Respondents
Medical Technologists are health professionals that analyze and test body fluids
(specifically blood) and tissues. They are knowledgeable about the tests and/or amount of blood
Research Instrument
The researchers used a survey questionnaire as their tool in this study, comprising two
parts.
Part I of the survey questionnaire provides the name of the Hospital (optional due to the
ethical issues), the type of the hospital which is either public or private and also the level of their
service provided whether it is a primary, secondary or a tertiary hospital. Followed by the different
Histopathology, Microbiology, Blood Banking or Serology .In part I the respondents will react by
checking the box of the appropriate answer beside each given option for each item.
Part II of the survey questionnaire elicited the different blood tests that are used in each lab
sections that are commonly achieved after obtaining the sample from the patient.There are 2
columns that the respondents need to answer, the volume extracted and volume used.
Part III of the survey requires the respondents to answer two the questions. First questions
is all about the factors that lead to blood wastage with six choices. Second question is all about the
ways of disposing blood with two choices. These two questions can be answered by checking the
choices.
Research Locale
This study will be conducted in Tacloban City for the following reasons; (1) there are
significant numbers of clinical laboratories in the city (2) there are different types/level of service
data are collected from all elements of the population. Total enumeration is used since intensive
investigation is needed and the number of members in the population is not large. This method is
expected to yield an accurate information since it measures the specific characteristics of the
respondents.
The researchers will make a letter for the approval to conduct the study in a Clinical
Laboratory within hospital. After which, the researchers will go to the clinical laboratoriy to
personally distribute the questionnaire to the medical practitioners who has the knowledge or
familiarity about the laboratory procedures. The questionnaire would help in determining if there
is blood wastage occurring in clinical laboratories. The questionnaire will be tallied and tabulated.
The data gathered from the questionnaire were analyzed according to their corresponding
quantitative equivalents. The responses were also categorized according to the nature of the
specified questions asked. Total enumeration was used for the profile of the respondents.
To compute for the mean for the responses of the respondents about the sample wastage
∑𝑥
𝑋̅ =
𝑛
Where: 𝑋̅ – Mean
CHAPTER 4
PRESENTATION, ANALYSIS AND INTERPRETATION OF DATA
This chapter presents the data gathered, the results of the statistical analysis done and
interpretation of findings. These are presented in tables and graphs following the sequence of
Tacloban City “.
Laboratory procedures that uses blood includes; haematology and coagulation, chemistry,
microbiology, transfusion and serologic tests. Haematology and coagulation tests have six specific
haematological tests and all are available in the hospital. Chemistry test have eight specific tests
but only seven are available . Microbiology and transfusion have four specific tests and all are
available. Serologic tests have eleven specific test but only three are available. A total of twelve
respondents answered the survey questionnaire and the mean response is showed in table 1 below.
Table 1.
(x)
A. Hematology
& Coagulation
CBC w/ or w/o 1.95 ml 0.5 ml 0.4 ml 1.55ml 79.5%
differential &
platelet count
Haematocrit or 1.6 ml 0.5 ml 0.2 ml 1.4 ml 88%
Hemoglobin or
both
1.8 ml 0.5ml 0.4 ml 1.4 ml 78%
WBC count w/
or w/o
differential 1.6 ml 0.5 ml 0.3 ml 1.3ml 81%
Platelet count
only 2.0 ml 1.8ml 0.5 ml 1.5ml 75%
PT and PTT, or
if ordered
singly
B. Chemistry
Tests
Individual 3 ml 0.6 ml 0.5 ml 2.5ml 83%
chemistry
analytes
Chem 10 panel 3 ml 0.6 ml 0.9 ml 2.1ml 70%
Met B panel 3 ml 0.6 ml 0.7 ml 2.3ml 77%
Met C panel 4 ml 1.2 ml 0.8 ml 3.2ml 80%
Lipid Profile 3 ml 0.6 ml 0.6 ml 2.4ml 80%
Chem 23 panel 4 ml 1.2 ml 3 ml 1 ml 25%
+ Lactate on
ice 4 ml 1.2 ml 0.8 ml 3.2ml 80%
Met C panel +
Lipid Panel +
CRP
C.
Microbiology
and 3.5 ml 1.0 ml 1.5 ml 2 ml 57%
Transfusion 3 ml 1.0 ml 1.3 ml 1.7ml 57%
Blood Culture
ABO Rh only 3 ml 1.5 ml 0.7 ml 2.3ml 77%
(Transfusion)
Type and 2.6 ml 0.5 ml 0.5 ml 2.1ml 81%
Screen (&ABO
Rh)
Blood Type
verification
D. Serologic
Tests
Syphilis 2.6 ml 1 ml 0.5 ml 2.1ml 81%
(Treponema
Pallidum)
Hepatitis B 2.6 ml 1 ml 0.3 ml 2.3ml 88%
Hepatitis C 2.3 ml 1 ml 0.4 ml 1.9ml 83%
This table presents the laboratory procedures, volume extracted, standard volume use,
dispensed blood, volume difference between the volume extracted and dispensed blood, and the
precent wastage.
Hematology and Coagulation. Significant percent wastage for all the tests can be
observed in the table. Haematocrit and Hemoglobin or both has the highest wastage with 88%
followed by platelet count only. CBC with or without differential and platelet count comes next
with 79.5% and then WBC count with or without differential at 78%. PT and APTT or if ordered
singly the least among them with 75% of wastage.
Chemistry. Chem panle 23 + Lactate on ice is the only tests where there is minimum
blood wastage compared to all the other test procedures with high percent wastage ranging from
70-80%.
Microbiology and Transfusion. Blood culture and ABO Rh and Transfusion has the
minimal blood wastage of 57%. Blood Type Verification and Type and Screen has a higher
percentage of blood wastage between 77-78%.
Serologic. There are three tests available in serology section. All test showed a highly
significant increase in blood wastage with minor differences form 81-88%.
Table 2.
Waste Imports 0 0%
Hemolyzed 4 33%
Total 12 100%
Blood Returned after 3o minutes and medically ordered but not used got the same
percent for about 17%.
Time expiry and Hemolysis got the highest responses for about 33%. This confirms that
the common factor that lead blood to wastage are due to time expiry and hemolysis.
Out of controlled temperature storage and wasted imports are not common factors that lead
blood sample to wastage.
Table 3: Ways of disposing blood
Treating with incineration is the most common way of disposing blood according the
mean responses for about 75%.
CHAPTER 5
Conclusion
From the data gathered, it was found out that sample wastage of blood happens in the
laboratory. The standard volume for extraction and the volume dispensed clearly showed a
significant difference resulting to an increase percent wastage for almost all the laboratory
procedures.
For hematology and coagulation, the volume for extraction ranges from 1 ml to 2 ml and
the dispensed from 00.2ml to 0.5 ml. CBC with of without differential & platelet count has the
Chemistry require a significant amount of blood so the volume of extraction is much higher
than the others. It ranges from 3 ml to 4ml and dispensed blood from 0.5ml to 3ml. Individual
chemistry anlaytes has the highest percent wastage of 83%. Chem 23+ panel+lactate on ice got
Microbiology and transfusion also require a significant amount of blood so a large volume
of sample is being drawn from a patient. The volume for extraction ranges from 2ml to 4 ml and
volume dispensed from 1ml to 2 ml. Compare to other laboratory procedures, microbio has lower
percent wastage. Blood type verification got the highest percent wastage of 81%.
Samples for serological testing are usually drawn together with chemistry examinations
making almost the same amount for volume of extraction. Sample wastage can be observed form
In general, hematology and serology laboratory procedures has the highest mean percent
wastage. Hematology at 80% and Serology at 84%. Sample wastage that can lead to more volume
of extraction may cause iatrogenic anemia in in-patients. As the study of Thavendiranathan et. al
suggest that the volume of blood draw strongly predicted decrease haemoglobin and haematocrit
levels. Before ordering blood request, physicians must always discern if it is necessary or not.
Blood wastage maybe due to many reasons. Time expiry and hemolysis are the common
reasons why blood sample is turned into waste according to the results that we have gathered.
Hemolysed and expired blood are not accepted in laboratory examinations because it causes false
results. Blood returned after 30 minutes and medically ordered but not used also contribute to
Blood waste must be properly disposed. Blood is an infectious fluid that can cause serious
illnesses. Many ways of properly disposing blood can be done by correct personnel. Treating with
incineration is the most common way according to the results that we have gathered followed by
If feasible, conduct the research in many hospital laboratories not just one.
Use standards specifically from our own country like from RITM or DOH.
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