Ink Chromatography
Ink Chromatography
Ink Chromatography
Erilla, Kcnorren C., Evangelista, Joven, Manalastas, Clain S., Palahang, Gadiel Elidave G.
Key words
1. INTRODUCTION
chemicals from which they are made. It can be used to separate mixtures like ink, blood,
gasoline, and lipstick. In ink chromatography, you are separating the colored pigments
that make up the color of the pen. Even though a pen will only write in one color, the ink
you put a small dot of ink to be separated at one end of a strip of filter paper. This end of
the paper strip is placed in a solvent. The solvent moves up the paper strip and, as it travels
upward, it dissolves the mixture of chemicals and pulls them up the paper. The chemicals
that dissolve best in the solvent will move up the paper strip further than chemicals that
do not dissolve as well. What is produced from this method is a chromatogram. Forensic
scientists can use ink chromatography to solve crimes by matching documents or stains
found at a crime scene to the marker or pen that belongs to a suspect. Forensic scientists
analyze the unknown ink and compare it with writing utensils collected from possible
suspects.
distance
a. speed = time
b. Rf (Top)
c. Rf (Bottom)
d. Rf (Center)
dTop+d Bottom
d center ( )
2
e. Rf of the solvent = -
d2 d2
To use paper chromatography to identify whether certain colored inks are pure
Figure 1
Figure 2
3. METHODOLOGY
This chapter clearly defines the research methods and procedures that will be used
in conducting the study. This discusses how the necessary data and information to address
the research objectives and questions will be collected, presented and analyzed.
Blue, Ink)
Procedure
from the longer edges of the paper. Measure the distance between
the two lines. This will be the distance traveled by the solvent front.
Note: label your filter paper using pencils, since the ink from your
2. Make a spot from the ink of each color pen onto a designated spot
on the filter paper, as shown in the figure below. Repeat this 3-5
times, to the same spot, to concentrate the dye on each spot. Allow
drying time between each application. Make sure that the spots will
remain small.
beaker. Staple the ends and the middle together to stabilize the
cylinder as shown in the figure below. Make sure that the flaps do
not overlap.
5. Slowly and carefully place the paper cylinder into the beaker making
sure that the bottom touches the ethanol solution uniformly. Make
sure that the rolled-up filter paper does not touch the walls of the
pulling along the components of the dye. When the solvent front
reaches the top line, remove the wet filter paper from the beaker.
10 cm
1.5 cm
Figure 3
(Prepared paper chromatography)