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Exercises12 PDF

This document contains exercises about spectrophotometry and electromagnetic radiation. It addresses topics like: - How frequency and wavelength relate to energy for electromagnetic radiation. - Molecular processes corresponding to different radiation energies. - The differences between transmittance, absorbance, and molar absorptivity and their relationships to concentration and wavelength. - Components of single-beam UV-VIS spectrophotometers like monochromators and photomultiplier tube detectors. - How spectrophotometers determine absorbance by measuring the intensity of radiation passing through a sample.

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Tran Thai
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44 views2 pages

Exercises12 PDF

This document contains exercises about spectrophotometry and electromagnetic radiation. It addresses topics like: - How frequency and wavelength relate to energy for electromagnetic radiation. - Molecular processes corresponding to different radiation energies. - The differences between transmittance, absorbance, and molar absorptivity and their relationships to concentration and wavelength. - Components of single-beam UV-VIS spectrophotometers like monochromators and photomultiplier tube detectors. - How spectrophotometers determine absorbance by measuring the intensity of radiation passing through a sample.

Uploaded by

Tran Thai
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Exercises for Spectrophotometry

1. Fill in the blanks for the following statements about electromagnetic radiation.

a) If you double the frequency, you ____________ the energy.

b) If you double the wavelength, you ____________ the energy.

2. Which molecular processes correspond to the energies of microwave, infrared,


visible and ultraviolet photons?

3. Explain the difference between transmittance, absorbance and molar


absorptivity. Which one(s) is/are proportional to concentration? Which one(s)
is/are dependent on wavelength?

4. What is an absorption spectrum?

5. Why does the iron-phenanthroline complex which strongly absorbs from 400-550
nm appear as red-orange?

6. What value of absorbance corresponds to 45.0 percent transmittance?

7. If a 0.0100 M solution has a percent transmittance of 45.0% at some wavelength,


what will be the percent transmittance for a 0.0200 M solution of this substance?

8. Identify two limitations of Beer’s law.

9. Dilution of a 4.95 x 10-3 M standard solution of a compound is made by taking


2.00 mL of this solution and diluting with solvent to 25.00 mL. The diluted
standard had an absorbance of 0.624 at 238 nm in a 1.00 cm cuvet. A blank
solution containing only solvent had an absorbance of 0.029 at 238 nm. The
absorbance of an unknown sample of this compound in the same solvent and
cuvet had an absorbance of 0.377. What is the concentration of the compound
in this sample? What is the molar absorptivity of this compound at 238 nm?
Exercises for Spectrophotometry

10. Identify the basic components of a single-beam UV-VIS spectrophotometer.

11. How does a monochromator allow only a narrow band of radiation to pass
through the sample in a single-beam spectrophotometer?

12. What are the major differences between a single-beam and a diode array
spectrophotometer?

13. Briefly describe how a photomultiplier tube detector converts radiation into an
electrical signal.

14. Describe how absorbance is determined by a spectrophotometer.

Solutions for Spectrophotometry Exercises

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