Analytical Estimation
Analytical Estimation
Analytical Estimation
, 1992)
Principle
DPPH radical is scavenged by antioxidants through the donation of a proton
forming the reduced DPPH. The colour change from purple to yellow after reduction
can be quantified by its decrease in absorbance at wavelength 517 nm.
Reagents
1. 0.2 mM DPPH
2. 80% Methanol
3. Butylated Hydroxy Anisole
Procedure
Various concentrations of ethanol & aqueous extract of the sample (4.0 ml)
were mixed with 1.0 ml of methanolic solution containing DPPH radicals, resulting in
the final concentration of DPPH being 0.2 mM. The mixture were shaken vigorously
and left to stand for 30 min, and the absorbance was measured at 517 nm. BHA was
used as control. The percentage of DPPH decolorization of the sample was calculated
according to the equation:
% decolorization = [1-(ABS sample /ABS control)] x 100
IC 50 value (mg extract/ml) is the inhibitory concentration at which DPPH
radicals were scavenged by 50 %. Ascorbic acid and BHA were used for comparison.
principle