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    A51a0f PDF

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    Komal
    This document contains 20 questions posed by Dr. Ajay Shankar of the Department of Chemistry at IGNTU. The questions cover topics related to spectrophotometry, including measuring absorbance, isobestic points, deconvolution of spectra, sources of false peaks, calculating transmittance and absorbance from photometer readings, properties of deuterium lamps and photomultiplier tubes, and applications of diffraction gratings. The questions are intended for chemistry students at both the undergraduate and graduate level.

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    A51a0f PDF

    Uploaded by

    Komal
    100 views3 pages
    This document contains 20 questions posed by Dr. Ajay Shankar of the Department of Chemistry at IGNTU. The questions cover topics related to spectrophotometry, including measuring absorbance, isobestic points, deconvolution of spectra, sources of false peaks, calculating transmittance and absorbance from photometer readings, properties of deuterium lamps and photomultiplier tubes, and applications of diffraction gratings. The questions are intended for chemistry students at both the undergraduate and graduate level.

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    This document contains 20 questions posed by Dr. Ajay Shankar of the Department of Chemistry at IGNTU. The questions cover topics related to spectrophotometry, including measuring absorbance, isobestic points, deconvolution of spectra, sources of false peaks, calculating transmittance and absorbance from photometer readings, properties of deuterium lamps and photomultiplier tubes, and applications of diffraction gratings. The questions are intended for chemistry students at both the undergraduate and graduate level.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    100 views3 pages

    A51a0f PDF

    Uploaded by

    Komal
    This document contains 20 questions posed by Dr. Ajay Shankar of the Department of Chemistry at IGNTU. The questions cover topics related to spectrophotometry, including measuring absorbance, isobestic points, deconvolution of spectra, sources of false peaks, calculating transmittance and absorbance from photometer readings, properties of deuterium lamps and photomultiplier tubes, and applications of diffraction gratings. The questions are intended for chemistry students at both the undergraduate and graduate level.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Collection of questions

    by
    Dr. Ajay Shankar
    Department of Chemistry, IGNTU

    1. The diagram shows how the absorbance is measured in a spectrophotometer. Will A1 and
    A2 be equal? Subscripts 1 and 2 are for different samples, and other notations have their
    usual meanings. Give the reason for your answer.

    2. What is the isobestic point?

    3. What do you mean by deconvolution of spectra?

    4. What is the source of false peak generally observed in UV-Vis spectroscopy?

    5. A spectrum of Ce(IV) obtained with a spectrophotometer having a glass optics (A) and
    quartz optics (B). Is peak (A) characteristic of the recorded sample in glass or something
    else?

    6. A portable photometer with a linear response to radiation registered 63.8 A with the
    solvent in the light path. The photometer was set to zero with no light striking the detector.
    Replacement of the solvent with an absorbing solution yielded a response of 41.6 A.
    Calculate
    (a) %T of sample solution.
    (b) absorbance of solution
    (c) transmittance to be expected for a solution in which the concentration of the absorber
    is one third that of the original solution.
    (d) transmittance to be expected for a solution that has twice the concentration of the
    sample solution.
    BSc- 1, 5, 6, 7, 8, 9, 10, 15, 17, 18, 19.
    MSc-All questions
    Collection of questions
    by
    Dr. Ajay Shankar
    Department of Chemistry, IGNTU

    7. A photometer with a linear response to radiation gave a reading of 498 mV with the solvent
    in the light path and 256 mV when the solvent was replaced by an absorbing solution. The
    photometer was set to zero with no light striking the detector. Calculate
    (a) the %T and absorbance of the absorbing solution.
    (b) the expected transmittance if the concentration of absorber is one half that of the
    original solution.
    (c) the transmittance to be expected if the light path through the original solution is doubled.

    8. Why does a deuterium lamp produce a continuum spectrum rather than a line spectrum in
    the UV region?

    9. Why can photomultiplier tubes not be used with IR radiation?

    10. Why is iodine sometimes introduced into a tungsten lamp?

    11. Why do quantitative and qualitative analyses often require different monochromator slit
    widths?

    12. Which part of the prism monochromator is responsible for chromatic aberrations? How it
    arises?

    13. Blazed gratings are used in monochromators which are different from normal gratings.
    Comment.

    14. What is blaze angle? Can it help in intensifying first order diffraction in grating
    monochromators? Are zero order radiations of any use in grating monochromators?

    15. Draw the cross-section of a diffraction grating showing incident angle, reflection angle,
    blaze angle, grating normal, grove normal, groove angle and grating spacing.

    16. Out of Ebert mounting and Czerny-Turner mounting, which one uses two mirrors in
    monochromators?

    17. A 20 ppm solution of a DNA molecule isolated from E. coli was found to give an
    absorbance of 0.80 in a 2 cm cell. Calculate the absorptivity of the DNA molecule.

    18. A compound of formula weight 180 has an absorptivity of 286 cm-1 g-1 L. What is its molar
    absorptivity?

    19. Calculate the missing information in the following table.


    BSc- 1, 5, 6, 7, 8, 9, 10, 15, 17, 18, 19.
    MSc-All questions
    Collection of questions
    by
    Dr. Ajay Shankar
    Department of Chemistry, IGNTU

    Grating Grooves/mm Wavelength


    A 600 nm
    B 1300
    C 1.2 m

    20. A solution with a true absorbance of 2.10 was placed in a spectrophotometer with a stray
    light percentage of 0.75. What absorbance would be measured?

    BSc- 1, 5, 6, 7, 8, 9, 10, 15, 17, 18, 19.


    MSc-All questions

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