A Qualitative Research

Name: Aldrin John E.
Paragat Section: STEM F
Literature Review
PLOT/ABSTRACT
In the past century, the recombinant DNA technology was just an imagination that
desirable characteristics can be improved in the living bodies by controlling the expressions of
target genes. However, in recent era, this field has demonstrated unique impacts in bringing
advancement in human life. By virtue of this technology, crucial proteins required for health
problems and dietary purposes can be produced safely, affordably, and sufficiently. This
technology has multidisciplinary applications and potential to deal with important aspects of
life, for instance, improving health, enhancing food resources, and resistance to divergent
adverse environmental effects. Particularly in agriculture, the genetically modified plants have
augmented resistance to harmful agents, enhanced product yield, and shown increased
adaptability for better survival. Moreover, recombinant pharmaceuticals are now being used
confidently and rapidly attaining commercial approvals. Techniques of recombinant DNA
technology, gene therapy, and genetic modifications are also widely used for the purpose of
bioremediation and treating serious diseases. Due to tremendous advancement and broad
range of application in the field of recombinant DNA technology, this review article mainly
focuses on its importance and the possible applications in daily life.
REFLECTION
Human life is greatly affected by three factors: deficiency of food, health problems, and
environmental issues. Food and health are basic human requirements beside a clean and safe
environment. With increasing world’s population at a greater rate, human requirements for
food are rapidly increasing. Humans require safe-food at reasonable price. Several human
related health issues across the globe cause large number of deaths. Approximately 36 million
people die each year from non communicable and communicable diseases, such as
cardiovascular diseases, cancer, diabetes, AIDS/HIV, tuberculosis, malaria, and several others
according to http://GlobalIssues.org/. Despite extensive efforts being made, the current world
food production is much lower than human requirements, and health facilities are even below
standard in the third-world countries. Rapid increase in industrialization has soared up the
environmental pollution and industrial wastes are directly allowed to mix with water, which has
affected aquatic marines and, indirectly, human-beings. Therefore, these issues urge to be
addressed through modern technologies. Unlike tradition approaches to overcome agriculture,
health, and environmental issues through breeding, traditional medicines, and pollutants
degradation through conventional techniques respectively, the genetic engineering utilizes
modern tools and approaches, such as molecular cloning and transformation, which are less
time consuming and yield more reliable products. For example, compared to conventional
breeding that transfers a large number of both specific and nonspecific genes to the recipient,
genetic engineering only transfers a small block of desired genes to the target through various
approaches, such as biotitic.
By the time I start reading the article, I’ve probably got a good idea of what Recombinant DNA is, how
rDNA is made, how rDNA works, and some general places where rDNA is currently important. I should
also be able to see that Recombinant DNA is going to have a large impact on the future. And like a lot of
new science technology, Recombinant DNA has the possibility to be used for "good" and "bad"
purposes. Recombinant DNA technology is an important development in science that has made
the human life much easier. In recent years, it has advanced strategies for biomedical
applications such as cancer treatment, genetic diseases, diabetes, and several plants disorders
especially viral and fungal resistance. The role of recombinant DNA technology in making
environment cleans (phytoremediation and microbial remediation) and enhanced resistance of
plants to different adverse acting factors (drought, pests, and salt) has been recognized widely.
The improvements it brought not only in humans but also in plants and microorganisms are
very significant. The challenges in improving the products at gene level sometimes face serious
difficulties which are needed to be dealt for the betterment of the recombinant DNA
technology future. In pharmaceuticals, especially, there are serious issues to produce good
quality products as the change brought into a gene is not accepted by the body. Moreover, in
case of increasing product it is not always positive because different factors may interfere to
prevent it from being successful. Considering health issues, the recombinant technology is
helping in treating several diseases which cannot be treated in normal conditions, although the
immune responses hinder achieving good results.
Several difficulties are encountered by the genetic engineering strategies which needed to be
overcome by more specific gene enhancement according to the organism’s genome. The
integration of incoming single-stranded DNA into the bacterial chromosome would be carried
out by a RecA-dependent process. This requires sequence homology between entities, the
bacterial chromosome and incoming DNA. Stable maintenance and reconstitution of plasmid
could be made easy. The introduction of genetic material from one source into the other is a
disaster for safety and biodiversity. There are several concerns over development of genetically
engineered plants and other products. For example, it is obvious that genetically engineered
plants can cross-breed with wild plants, thus spreading their “engineered” genes into the
environment, contaminating our biodiversity. Further, concerns exist that genetic engineering
has dangerous health implications. Thus, further extensive research is required in this field to
overcome such issues and resolve the concerns of common people.

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Name: Aldrin John E.

Paragat Section: STEM F

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